scholarly journals Metabolomics in psoriatic disease: pilot study reveals metabolite differences in psoriasis and psoriatic arthritis

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 248 ◽  
Author(s):  
April W. Armstrong ◽  
Julie Wu ◽  
Mary Ann Johnson ◽  
Dmitry Grapov ◽  
Baktazh Azizi ◽  
...  
Keyword(s):  
Psoriatic Arthritis ◽  
Skin Diseases ◽  
Healthy Controls ◽  
Serum Samples ◽  
Inflammatory Skin Diseases ◽  
Flight Mass Spectrometry ◽  
Therapeutic Development ◽  
And Gender ◽  
The University ◽  
Study Participants

Importance: While “omics” studies have advanced our understanding of inflammatory skin diseases, metabolomics is mostly an unexplored field in dermatology.Objective: We sought to elucidate the pathogenesis of psoriatic diseases by determining the differences in metabolomic profiles among psoriasis patients with or without psoriatic arthritis and healthy controls.Design: We employed a global metabolomics approach to compare circulating metabolites from patients with psoriasis, psoriasis and psoriatic arthritis, and healthy controls.Setting: Study participants were recruited from the general community and from the Psoriasis Clinic at the University of California Davis in United States.Participants: We examined metabolomic profiles using blood serum samples from 30 patients age and gender matched into three groups: 10 patients with psoriasis, 10 patients with psoriasis and psoriatic arthritis and 10 control participants.Main outcome(s) and measures(s): Metabolite levels were measured calculating the mean peak intensities from gas chromatography time-of-flight mass spectrometry.Results: Multivariate analyses of metabolomics profiles revealed altered serum metabolites among the study population. Compared to control patients, psoriasis patients had a higher level of alpha ketoglutaric acid (Pso: 288 ± 88; Control: 209 ± 69; p=0.03), a lower level of asparagine (Pso: 5460 ± 980; Control: 7260 ± 2100; p=0.02), and a lower level of glutamine (Pso: 86000 ± 20000; Control: 111000 ± 27000; p=0.02). Compared to control patients, patients with psoriasis and psoriatic arthritis had increased levels of glucuronic acid (Pso + PsA: 638 ± 250; Control: 347 ± 61; p=0.001). Compared to patients with psoriasis alone, patients with both psoriasis and psoriatic arthritis had a decreased level of alpha ketoglutaric acid (Pso + PsA: 186 ± 80; Pso: 288 ± 88; p=0.02) and an increased level of lignoceric acid (Pso + PsA: 442 ± 280; Pso: 214 ± 64; p=0.02).Conclusions and relevance: The metabolite differences help elucidate the pathogenesis of psoriasis and psoriatic arthritis and they may provide insights for therapeutic development.

scholarly journals Apolipoprotein M Serum Levels Correlate with IgA Vasculitis and IgA Vasculitis Nephritis

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Jiali Wu ◽  
Lagu He ◽  
Le Bai ◽  
Li Tan ◽  
Min Hu
Keyword(s):  
Protective Factor ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Iga Vasculitis ◽  
Independent Predictive Factor ◽  
Apolipoprotein M ◽  
Biochemical Analyzer ◽  
And Gender ◽  
Study Participants

Objective. IgA vasculitis (lgAV) is the most frequent vessel vasculitis in children, and the prognosis is related to the children’s age and degree of nephritis. This study is aimed at investigating serum apolipoprotein M (apoM) levels in patients with lgAV patients and at evaluating the association between apoM and disease severity. Methods. A total of 109 lgAV patients and 76 age- and sex-matched healthy controls were included. The age and gender of the study participants were matched. ApoM levels were measured by an enzyme-linked immunosorbent assay. Additionally, the serum levels of lipids, apolipoproteins, kidney biochemical profiles, immunoglobulins (IgA, IgG, IgM, and IgE), and the complements (C3 and C4) were assessed using an automatic biochemical analyzer. Results. ApoM was increased significantly in lgAV patients compared to healthy controls. ApoM, meanwhile, was lower in patients with nephritis than in those without nephritis. The apoM levels were higher in classes I and II IgA vasculitis nephritis (lgAVN) patients than in classes III and IV. Besides, the apoM serum level<24.81 mg/L was an independent predictive factor for lgAVN and can be independently associated with the presence of nephritis in lgAV patients. Meanwhile, the serum apoM concentration negatively correlated with the ISKDC grading score in lgAVN patients. Conclusions. Serum apoM was elevated in lgAV patients and decreased gradually with the ISKDC grading score. ApoM (OR=0.32, 95%CI=0.12‐0.85, p=0.023) was identified as a protective factor for nephritis in all lgAV patients.

Persistent deficiency of mucosal-associated invariant T cells during dermatomyositis

Rheumatology ◽  
2019 ◽  
Vol 59 (9) ◽  
pp. 2282-2286 ◽  
Author(s):  
Charles Cassius ◽  
Mylene Branchtein ◽  
Maxime Battistella ◽  
Reyhan Amode ◽  
Clémence Lepelletier ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Skin Diseases ◽  
Cytotoxic T Lymphocyte ◽  
Low Frequency ◽  
Inactive Disease ◽  
Peripheral Blood Flow ◽  
Healthy Controls ◽  
Inflammatory Skin Diseases ◽  
Mait Cells ◽  
Mait Cell

Abstract Objectives Mucosal-associated invariant T (MAIT) cells are innate-like lymphocytes that are important for antibacterial immunity and may have regulatory roles. MAIT cells are decreased during SLE. However, their frequencies and phenotype have not been investigated in DM. We studied MAIT cell frequencies and phenotype in DM patients with active and inactive disease (after treatment). Methods Peripheral blood flow cytometry analysis of MAIT cells was compared between DM (n = 22), SLE (n = 10), psoriasis (n = 7) and atopic dermatitis (n = 5) patients, and healthy controls (n = 19). Results A dramatic decrease of circulating MAIT cell frequency was observed in active DM and SLE patients compared with healthy controls and other inflammatory skin diseases [active DM: median = 0.25% (interquartile range 0.19–0.6%), P &lt; 0.0001; active SLE: median = 0.61 (0.55–0.77), P &lt; 0.0001 vs healthy controls: 2.32% (1.18–4.45%)]. MAIT cells from active DM patients had an abnormal phenotype including increased expression of CD25 and cytotoxic T-lymphocyte-associated protein 4 that correlated with their low frequency in the blood. Conclusion In DM, peripheral blood MAIT cells are dramatically reduced and have an activated/exhausted phenotype that may be linked to increased activation-induced cell death.

scholarly journals Energy Intake and Satiety Responses of Eggs for Breakfast in Overweight and Obese Adults—A Crossover Study

2020 ◽  
Vol 17 (15) ◽  
pp. 5583 ◽  
Author(s):  
Jennifer B Keogh ◽  
Peter M Clifton
Keyword(s):  
Energy Intake ◽  
Dietary Advice ◽  
Obese Adults ◽  
Breakfast Meal ◽  
Time Interaction ◽  
Different Populations ◽  
And Gender ◽  
The University ◽  
Study Participants ◽  
Food Consumed

The type of food eaten for breakfast may determine the amount of food consumed at the next meal. This may be important when considering dietary advice for overweight and obese individuals who are trying to lose weight. The aim of the study was to investigate the energy intake and subjective sensations of hunger using a visual analogue scale (VAS) of a breakfast meal of eggs compared with a breakfast meal of cereal in overweight Australian adults. In a cross-over study, participants attended the University of South Australia’s Clinical Trial Facility on two separate days, one week apart. On each day participants consumed one of two isoenergetic breakfasts (1800 kJ), either eggs and toast or cereal with milk and orange juice. Fifty overweight or obese participants, 44 ± 21 years, 86 ± 14 kg, with a body mass index (BMI) of 31 ± 4 kg/m2 completed both study visits. Energy intake following the egg breakfast was significantly reduced compared with the cereal breakfast (4518 vs. 5283 kJ, p = 0.001). BMI and gender were unrelated to these effects. The sensation of hunger was less after the egg breakfast (p = 0.028 for diet by time interaction) and returned more quickly after the cereal breakfast. There were no effects of gender or age. Energy intake was reduced at an ad libitum lunch meal 4 hours after a breakfast meal containing eggs. The findings suggest that satiety responses of overweight and obese are not different to non-obese participants as our study confirms findings from studies conducted in different populations. Determining which foods may help overweight and obese individuals manage their food intake is important for diet planning.

scholarly journals B-26 Predictors of Cognitive Decline Over a 2-year Follow up: Olfaction and Scans Without Evidence of Dopaminergic Deficit Status (SWEDD)

2019 ◽  
Vol 34 (6) ◽  
pp. 972-972
Author(s):  
F Lopez ◽  
B Rohl ◽  
A Wagle Shukla ◽  
D Bowers
Keyword(s):  
Cognitive Decline ◽  
Total Sample ◽  
University Of Pennsylvania ◽  
Dopamine Depletion ◽  
High Discriminatory Power ◽  
Progression Markers ◽  
And Gender ◽  
The University ◽  
Study Participants

Abstract Objective Given the high discriminatory power of olfaction assessments in movement disorders, the current study sought to determine whether olfaction dysfunction differentially predicted cognitive decline in patients who have scans without evidence of dopaminergic deficit (SWEDD). Dopamine depletion is a major neuropathological feature of Parkinson’s disease (PD); however, 15% of patients with a clinical PD diagnosis have neuroimaging evidence of intact dopaminergic function. Recent work has suggested that patients with SWEDD are at a greater risk of cognitive impairment relative to those with PD. Methods Archival data were obtained from the Parkinson’s Progression Markers Initiative database. The total sample included three groups: PD (n = 401), SWEDD (n = 51), and HC (n = 175). Participants were categorized into non-impaired (-) or impaired (+) olfaction groups based on performance on the University of Pennsylvania Smell Identification Test, corrected for age and gender. Participants were administered the Montreal Cognitive Assessment twice and change scores were calculated to examine changes in cognition over time (baseline – two-year follow-up). Results A bootstrapped analysis of variance (ANOVA) revealed that the SWEDD+ group had lower MoCA scores than PD+ and HC+ groups at baseline (ps < .05). A bootstrapped ANOVA indicated that group differences were not significant at follow-up; rather, impaired baseline olfaction predicted cognitive decline across all study participants (p < .05), regardless of diagnosis. Conclusions Future studies are needed to assess whether the profile of motor and non-motor symptoms in SWEDD patients, including olfaction, is deserving of its own syndrome, or whether individual patients may fit better under alternative, existing diagnoses.

scholarly journals Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection

2021 ◽  
Vol 11 ◽  
Author(s):  
Johannes J. Kovarik ◽  
Anna K. Kämpf ◽  
Fabian Gasser ◽  
Anna N. Herdina ◽  
Monika Breuer ◽  
...  
Keyword(s):  
Immune Activation ◽  
Early Onset ◽  
Healthy Controls ◽  
Serum Samples ◽  
Cross Sectional ◽  
Activation Markers ◽  
Luminex Technology ◽  
Patient Groups ◽  
And Gender ◽  
Medical University

This study aimed to determine the specific cytokine profile in peripheral blood during the early onset of COVID-19 infection. This was a cross-sectional exploratory, single center study. A total of 55 plasma samples were studied. Serum samples of adults showing symptoms of COVID-19 infection who were tested positive for SARS-CoV-2 infection (CoV+, n=18) at the COVID-19 outpatient clinic of the Medical University of Vienna were screened for immune activation markers by Luminex technology. Additionally, age and gender-matched serum samples of patients displaying COVID-19 associated symptoms, but tested negative for SARS-CoV-2 (CoV-, n=16) as well as healthy controls (HC, n=21) were analyzed. COVID-19 positive (CoV+) patients showed a specific upregulation of BLC (141; 74-189 pg/mL), SCD30 (273; 207-576 pg/mL), MCP-2 (18; 12-30 pg/mL) and IP-10 (37; 23-96 pg/mL), compared to patients with COVID19-like symptoms but negative PCR test (CoV-), BLC (61; 22-100 pg/mL), sCD30L (161; 120-210 pg/mL), MCP-2 (8; 5-12 pg/mL) and IP-10 (9; 6-12 pg/mL) and healthy controls (HC) (BLC 22; 11-36 pg/mL, sCD30 74; 39-108 pg/mL, MCP-2 6; 3-9. pg/mL, IP-10 = 8; 5-13). The markers APRIL, sIL-2R, IL7, MIF, MIP-1b, SCF, SDF-1a, sTNF-RII were elevated in both CoV+ and CoV- patient groups compared to healthy controls. HGF, MDC and VEGF-A were elevated in CoV- but not CoV+ compared to healthy controls. BLC, sCD30, MCP-2 and IP-10 are specifically induced during early stages of COVID-19 infection and might constitute attractive targets for early diagnosis and treatment of this disease.

scholarly journals Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maja-Lisa Clausen ◽  
S. Kezic ◽  
C. M. Olesen ◽  
T. Agner
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Skin Diseases ◽  
Sampling Strategy ◽  
Tape Stripping ◽  
Healthy Controls ◽  
Inflammatory Skin Diseases ◽  
Lesional Skin ◽  
Cytokine Milieu ◽  
Healthy Control

AbstractTape stripping is a promising technique for assessment of epidermal biomarkers in inflammatory skin diseases. However, to facilitate its implementation in the clinical practice, a thorough validation regarding sampling strategy is needed. Knowledge of biomarkers variation in concentration across stratum corneum is scarce. Therefore, this study aimed to assess the variability of cytokines across stratum corneum using tape stripping technique by consecutive application of 21 adhesive tapes (D-squame) to lesional and non-lesional skin from 15 patients with atopic dermatitis (AD) and 16 healthy controls. Concentration of cytokines (IL-1α, IL-1b, IL-5, IL-18, IFN-γ, CCL17, CCL22, CCL27, CXCL8, CXCL10, TNF-α, TSLP, VEGFA) was determined in five different depths, using multiplex immunoassay. Comparing tape 4 with tape 21, no cytokine changed significantly in concentration in AD lesional skin. In AD non-lesional skin a small decrease was found for CCL17, CXCL8 and CXCL10. For healthy controls, a decrease was found for IL-1a, IL-1b, VEGFA and an increase for IL-18. Differences were found between AD skin and healthy control skin. Concentration of cytokines was stable across stratum corneum, indicating that sampling of only one tape from the stratum corneum is reliable in reflecting the overall cytokine milieu. Differences between AD and healthy skin confirm robustness of tape stripping for measuring cytokine levels.

scholarly journals 1H NMR Based Metabolomics in Human Sepsis and Healthy Serum

Metabolites ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 70 ◽  
Author(s):  
Henna Jaurila ◽  
Vesa Koivukangas ◽  
Marjo Koskela ◽  
Fiia Gäddnäs ◽  
Sami Myllymaa ◽  
...  
Keyword(s):  
1H Nmr ◽  
Serum Levels ◽  
Serum Lactate ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Healthy Controls ◽  
Serum Samples ◽  
Human Sepsis ◽  
Metabolite Concentrations ◽  
And Gender

Early diagnosis is essential but challenging in severe sepsis. Quantifying and comparing metabolite concentrations in serum has been suggested as a new diagnostic tool. Here we used proton nuclear magnetic resonance spectroscopy (1H NMR) based metabolomics to analyze the possible differences in metabolite concentrations between sera taken from septic patients and healthy controls, as well as between sera of surviving and non-surviving sepsis patients. We took serum samples from 44 sepsis patients when the first sepsis induced organ dysfunction was found. Serum samples were also collected from 14 age and gender matched healthy controls. The samples were analyzed by quantitative 1H NMR spectroscopy for non-lipid metabolites. We found that the serum levels of glucose, glycine, 3-hydroxybutyrate, creatinine and glycoprotein acetyls (mostly alpha-1-acid glycoprotein, AGP) were significantly (p < 0.05) higher in sepsis compared to healthy sera, whereas citrate and histidine were significantly (p < 0.05) lower in sepsis patients compared to healthy controls. We found statistically significantly higher serum lactate and citrate concentrations in non-survivors compared to 30-day survivors. According to our study, 3-hydroxybutyrate, citrate, glycine, histidine, and AGP are candidates for further studies to enable identification of phenotype association in the early stages of sepsis.

scholarly journals Serum metabolic fingerprinting of psoriasis and psoriatic arthritis patients using solid-phase microextraction—liquid chromatography—high-resolution mass spectrometry

Metabolomics ◽  
2021 ◽  
Vol 17 (7) ◽  
Author(s):  
Nikita Looby ◽  
Anna Roszkowska ◽  
Nathaly Reyes-Garcés ◽  
Miao Yu ◽  
Tomasz Bączek ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Psoriatic Arthritis ◽  
Solid Phase Microextraction ◽  
High Resolution Mass Spectrometry ◽  
Solid Phase ◽  
Healthy Controls ◽  
Serum Samples ◽  
Resolution Mass

Abstract Introduction Psoriatic arthritis (PsA), an inflammatory arthritis that develops in individuals with psoriasis, is associated with reduced quality of life. Identifying biomarkers associated with development of PsA as well as with PsA disease activity may help management of psoriatic disease. Objectives To use metabolomic fingerprinting to determine potential candidate markers of disease conversion (psoriasis to PsA) and/or PsA activity. Methods A novel sample preparation protocol based on solid-phase microextraction (SPME) was used to prepare serum samples obtained from: (1) individuals with psoriasis, some of whom develop psoriatic arthritis (n = 20); (2) individuals with varying PsA activity (mild, moderate, severe; n = 10 each) and (3) healthy controls (n = 10). Metabolomic fingerprinting of the obtained extracts was performed using reversed-phase liquid chromatography coupled to high resolution mass spectrometry. Results Psoriasis patients who developed PsA had similar metabolomic profiles to patients with mild PsA and were also indistinguishable from patients with psoriasis who did not develop PsA. Elevated levels of selected long-chain fatty acids (e.g., 3-hydroxytetradecanedioic acid) that are associated with dysregulation of fatty acid metabolism, were observed in patients with severe PsA. In addition, 1,11-undecanedicarboxylic acid—an unusual fatty acid associated with peroxisomal disorders—was also identified as a classifier in PsA patients vs. healthy individuals. Furthermore, a number of different eicosanoids with either pro- or anti-inflammatory properties were detected solely in serum samples of patients with moderate and severe PsA. Conclusion A global metabolomics approach was employed to analyze the serum metabolome of patients with psoriasis, PsA, and healthy controls in order to examine potential differences in the biochemical profiles at a metabolite level. A closer examination of circulating metabolites may potentially provide markers of PsA activity.

scholarly journals Treatment of Atopic Dermatitis Using a Full-Body Blue Light Device (AD-Blue): Protocol of a Randomized Controlled Trial (Preprint)

2018 ◽  
Author(s):  
Christian Kromer ◽  
Viktoria P Nühnen ◽  
Wolfgang Pfützner ◽  
Sebastian Pfeiffer ◽  
Hans-Joachim Laubach ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Atopic Dermatitis ◽  
Blue Light ◽  
Skin Diseases ◽  
Controlled Trial ◽  
Ethics Committee ◽  
Inflammatory Skin Diseases ◽  
Randomized Controlled ◽  
The University ◽  
Full Body

BACKGROUND Irradiation with visible blue light (wavelength 400-495 nm) is a promising, effective, and safe new treatment option for chronic inflammatory skin diseases such as psoriasis and atopic dermatitis. OBJECTIVE We will perform a multicenter, placebo-controlled, double-blinded, 3-armed, prospective, randomized controlled trial to investigate the efficacy and safety of full-body blue light devices (wavelengths: 415 nm and 450 nm) compared with that of placebo irradiation for the treatment of atopic dermatitis. METHODS We are planning to enroll a total of 150 patients at the University hospitals in Göttingen (Germany), Marburg (Germany), and Geneva (Switzerland). RESULTS The trial was approved by the lead ethics committee of the medical faculty of the University of Göttingen (21/11/16). Further approvals were obtained from local and federal authorities (ethics committee Marburg, Cantonal Commission for Research Ethics Geneva, Suisse Medic, and Bundesinstitut für Arzneimittel und Medizinprodukte). CONCLUSIONS We will disseminate the results in a peer-reviewed journal. CLINICALTRIAL ClinicalTrials.gov NCT03085303; https://clinicaltrials.gov/ct2/show/NCT03085303 (Archived by WebCite at http://www.webcitation.org/73ucqkkA1) INTERNATIONAL REGISTERED REPOR PRR1-10.2196/11911

Study the Relationship Between Estrogen Hormone and Calcium in Normal Females Before and After Menopause in Baghdad / Iraq

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Sameerah Mustafa ◽  
Asal Tawfeeq ◽  
Hadeel Hasan
Keyword(s):  
Oral Contraceptive ◽  
Healthy Controls ◽  
Serum Samples ◽  
Oral Contraceptive Pills ◽  
Contraceptive Pills ◽  
Menopausal Women ◽  
Before And After ◽  
Group A ◽  
Group B ◽  
Age Range

This study involved the collection of (90) samples of women serum which included (30) serum samples collected from women before menopause (reproductive women) in the age range of (22-43) years and were considered as (group A- control). While, (group B) included (30) serum samples collected from women using oral contraceptive pills between the ages of (22-43) years old. Whereas, another (30) serum samples were collected from women after menopause between the ages of (43-54) years and were considered as (group C). All of the collected serum samples were subjected to a number of serological and chemical tests for the measurement of (E2, HDL, LDL and Ca). Then, the obtained data were statistical analyzed and results showed a significant decrease (p˂ 0.05) in (E2 ,Ca and HDL) levels in menopausal women compared to that of the normal healthy controls. While, there were non-significant decrease (p> 0.05) in (E2, Ca and HDL) levels in women taking oral contraceptive when compared to the normal healthy controls. On the other hand, a significant increase (p˂ 0.05) was recorded in LDL level in menopausal women compared to that of the normal healthy controls whereas, no-significant increase (p˃ 0.05) in the LDL level in women taking oral contraceptives when compared to the control women.

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