scholarly journals Marine plant mediated green synthesis of silver nanoparticles using mangrove Rhizophora stylosa: Effect of variable process and their antibacterial activity

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 768
Author(s):  
Nancy Willian ◽  
Syukri Syukri ◽  
Zulhadjri Zulhadjri ◽  
Syukri Arief
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Narrow Particle Size Distribution ◽  
Metallic Silver ◽  
Face Centered Cubic ◽  
Rhizophora Stylosa ◽  
Vis Spectroscopy

Background: Most natural plants used in the synthesis of silver nanoparticles are limited to marine plants. To carry out applications, colloidal silver nanoparticles (AgNps) should have appropriate properties such as homogeneous shapes, small and narrow particle size distribution, and long time stability. This study aims to determine the effects of a variable process of AgNps mediated mangrove Rhizophora stylosa (RS) leaf extract, and antibacterial activity.  Methods: Synthesis of AgNps was carried out by stirring silver nitrate solution with aqueous extract. The characterization of AgNps was carried out using UV-Vis spectrophotometry, X-ray diffraction (XRD), Dynamic Light Scattering (DLS) zetasizer and Transmission Electron Microscopy (TEM). Evaluation of antibacterial activity was carried out on Escherichia coli and Staphylococcus aureus. Reaction conditions such as the concentration of metal ions (0.001 M, 0.005 M, and 0.01 M), extracts (1%, 3%, and 5% v/v), and the reaction time on the size and stability of nanoparticles were also explored. Results: The UV-Vis spectroscopy showed an absorption of colloidal AgNps in a wavelength range of 403–443 nm.  TEM analysis showed that as-synthesized AgNps were spherical in shape with a size range of 5–87 nm. The use of 0.001 M and 0.005 M of Ag+ resulted in a smaller diameter than the synthesized AgNps, using 0.01 M Ag+, in the same extract concentration. The range of zeta potential was -24.9 mV to -27.7 mV. The as-synthesized AgNps were stable for more than one month. The XRD analysis showed four peaks, which were attributed to the face centered cubic crystal structure of metallic silver. The results of the silver nanoparticles synthesis showed good activity on Escherichia coli and Staphylococcus aureus, with an inhibition zone between 4.1–7.2 mm. Conclusions: The AgNps synthesized with RS leaf extract, which is a reducing agent, showed good potential as an antibacterial component.

scholarly journals Highly Antibacterial Activity Against Methicillin-resistant Staphylococcus aureus of Silver Nanoparticles Synthesized by Citrus maxima Peel Extract

2021 ◽  
Author(s):  
Thu Ha Bui ◽  
Ngoc Dai Nghia Tran ◽  
Phung Anh Nguyen ◽  
Nhat Linh Duong ◽  
Van Minh Nguyen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Average Diameter ◽  
Reducing Agent ◽  
Face Centered Cubic ◽  
Methicillin Resistant ◽  
Vis Spectroscopy ◽  
Citrus Maxima

Abstract A cost-effective and green technique was performed for the synthesis of silver nanoparticles (AgNPs) from a plant resource using Citrus maxima peel (CMP) extract as a reducing agent. The formation of AgNPs was confirmed by UV-Vis Spectroscopy at the wavelength range of 400−500 nm. The optimized conditions for the AgNPs synthesis using CMP extract as a reducing agent were determined. At these conditions, the X-ray diffraction (XRD) and the high-resolution transmission electron microscopy (HRTEM) results revealed the face-centered cubic structure of AgNPs had a highly crystalline with the particle size in a range of 10−20 nm. The Fourier transform infrared spectroscopy (FT-IR) demonstrated the presence of flavonoid, terpenoid, phenolic, and glycosides in phytochemical compositions of CMP extract which can act as the reducing agents for AgNPs formation. The antibacterial effect of the AgNPs was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA) by implementing the minimum inhibitory concentration (MIC), minimum batericidal concentration (MBC), and the zone of inhibition tests. The AgNPs exhibited effective antibacterial activity against bacteria with an average diameter of inhibition zones of 11.7 mm, the MIC of 8.27 µg/mL, and the MBC of the 16.54 µg/mL.

scholarly journals Green Synthesis of Silver Nanoparticles Using Moringa Oleifera Leaf Extract, Tamarindus Indica Fruit Extract and Assessement of Antibacterial Activity Against Staphylococcus Aureus

2021 ◽  
Author(s):  
Shirisha A ◽  
ANUMOLU VIJAYA KUMAR ◽  
Laxman Chatlod R ◽  
Shashi Kumar M ◽  
Krishnaiah N ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Diffusion Method ◽  
Tamarindus Indica ◽  
Fruit Extract

Abstract The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this study for synthesis of silver nanoparticles different ratios of 1mM silver nitrate and Moringa oleifera leaf extract i.e, 95:5, 90:10 and 85: 15 was taken in conical flask and kept for one 1 hr at 25 0 c on magnetic stirrer, out of which 90:10 ratio was selected for further study based on highest peak, good size and stability. Tamarindus indica fruit extract was added to silver nitrate solution till the colour of the solution changes from light brown to chocolate brownish colour. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy, Zeta potential, size distribution by intensity. The absorption spectrum of the silver nano solution prepared by using Moringa oleifera and Tamarindus indica fruit extract showed a surface plasmon absorption band with maximum of 420 nm and 430 nm respectively indicating the presence of silver nanoparticles. The zeta value of silver nanoparticles synthesized from Moringa oleifera and Tamarindus indica fruit extract was -12.5 mV and -15.5 mV, size of 110.2 nm and 130.2 nm respectively. The antibacterial efficacy of nanosilver was checked by agar well diffusion method, and the silver nanoparticles showed effective antibacterial activity against Staphylococcus aureus.

scholarly journals Synthesis, Characterization, and Evaluation of the Antibacterial Activity ofAllophylus serratusLeaf and Leaf Derived Callus Extracts Mediated Silver Nanoparticles

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Kero Jemal ◽  
B. V. Sandeep ◽  
Sudhakar Pola
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Eds Analysis ◽  
Vis Spectroscopy ◽  
Absorbance Peak ◽  
Face Centered ◽  
Spectroscopy Studies

Allophylus serratusmediated silver nanoparticles biosynthesis, characterization, and antimicrobial activity were described. The synthesis of silver nanoparticles was confirmed by visual observation: UV-Vis spectrum, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), and Fourier Transform Infra-Red (FTIR). UV-Vis spectroscopy studies showed that the absorption spectra of synthesized silver nanoparticles from leaf and callus extracts had absorbance peak range of 440 nm and 445 nm, respectively. The X-RD pattern revealed the presence of crystalline, dominantly spherical silver nanoparticles in the sample having size ranging from 42 to 50 nm. The XRD peaks 38.2°, 44.1°, 64.1°, and 77.0° for leaf extract and 38.1°, 44.3°, 64.5°, 77.5°, and 81.33° for callus extract can be assigned the plane of silver crystals (111), (200), (220), and (311), respectively, and indicate that the silver nanoparticles are face-centered, cubic, and crystalline in nature. SEM and EDS analysis also confirmed the presence of silver nanoparticles. The FTIR results showed the presence of some biomolecules in extracts that act as reducing and capping agent for silver nanoparticles biosynthesis. The synthesized silver nanoparticles showed significant antibacterial activity againstKlebsiella pneumoniaeandPseudomonas aeruginosa.

scholarly journals Investigation of Nanoparticle Metallic Core Antibacterial Activity: Gold and Silver Nanoparticles against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 22 (4) ◽  
pp. 1905
Author(s):  
Jimmy Gouyau ◽  
Raphaël E. Duval ◽  
Ariane Boudier ◽  
Emmanuel Lamouroux
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metallic Nanoparticles ◽  
Catalytic Reduction ◽  
Gold And Silver Nanoparticles ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Broth Microdilution Method

Multidrug-resistant (MDR) bacteria constitute a global health issue. Over the past ten years, interest in nanoparticles, particularly metallic ones, has grown as potential antibacterial candidates. However, as there is no consensus about the procedure to characterize the metallic nanoparticles (MNPs; i.e., metallic aggregates) and evaluate their antibacterial activity, it is impossible to conclude about their real effectiveness as a new antibacterial agent. To give part of the answer to this question, 12 nm gold and silver nanoparticles have been prepared by a chemical approach. After their characterization by transmission electronic microscopy (TEM), Dynamic Light Scattering (DLS), and UltraViolet-visible (UV-vis) spectroscopy, their surface accessibility was tested through the catalytic reduction of the 4-nitrophenol, and their stability in bacterial culture medium was studied. Finally, the antibacterial activities of 12 nm gold and silver nanoparticles facing Staphylococcus aureus and Escherichia coli have been evaluated using the broth microdilution method. The results show that gold nanoparticles have a weak antibacterial activity (i.e., slight inhibition of bacterial growth) against the two bacteria tested. In contrast, silver nanoparticles have no activity on S. aureus but demonstrate a high antibacterial activity against Escherichia coli, with a minimum inhibitory concentration of 128 µmol/L. This high antibacterial activity is also maintained against two MDR-E. coli strains.

scholarly journals Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 487 ◽  
Author(s):  
Abdalrahim Alahmad ◽  
Armin Feldhoff ◽  
Nadja C. Bigall ◽  
Pascal Rusch ◽  
Thomas Scheper ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Hypericum Perforatum ◽  
Nitrate Solution ◽  
A549 Cells ◽  
Metallic Silver ◽  
Synthesis Methods ◽  
Face Centered Cubic ◽  
Sem Images ◽  
Hypericum Perforatum L

This contribution focuses on the green synthesis of silver nanoparticles (AgNPs) with a size < 100 nm for potential medical applications by using silver nitrate solution and Hypericum Perforatum L. (St John’s wort) aqueous extracts. Various synthesis methods were used and compared with regard to their yield and quality of obtained AgNPs. Monodisperse spherical nanoparticles were generated with a size of approximately 20 to 50 nm as elucidated by different techniques (SEM, TEM). XRD measurements showed that metallic silver was formed and the particles possess a face-centered cubic structure (fcc). SEM images and FTIR spectra revealed that the AgNPs are covered by a protective surface layer composed of organic components originating from the plant extract. Ultraviolet-visible spectroscopy, dynamic light scattering, and zeta potential were also measured for biologically synthesized AgNPs. A potential mechanism of reducing silver ions to silver metal and protecting it in the nanoscale form has been proposed based on the obtained results. Moreover, the AgNPs prepared in the present study have been shown to exhibit a high antioxidant activity for 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation, and super oxide anion radical and 2,2-diphenyl-1-picrylhydrazyl. Synthesized AgNPs showed high cytotoxicity by inhibiting cell viability for Hela, Hep G2, and A549 cells.

Green Synthesis and Characterization of Silver Nanoparticles by Using Aloe Vera Leaf Extract to Study the Effects of Synthesis Parameters

2016 ◽  
Vol 860 ◽  
pp. 179-184
Author(s):  
Asif Rahman ◽  
A.K.M. Bazlur Rashid ◽  
Md Abdul Aziz Antor ◽  
Md Ashif Anwar ◽  
Roisul Hasan Galib
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Aloe Vera ◽  
Bath Temperature ◽  
Heating Time ◽  
Water Bath ◽  
Metallic Silver ◽  
Nanoparticle Suspension

In this research silver nanoparticles were synthesized by reducing silver nitrate solution using aloe vera leaf extract as reducing agent. The synthesized nanoparticles had been characterized and the effects of different parameters of synthesis had been evaluated. Silver nanoparticles began to form just after reaction and the whole reduction reaction was completed within 3 hours. The color changed from transparent to dark brown of the aqueous salt solution. Later the nanoparticles were separated out from the mixture by ultra-centrifugation. Here the effects of water bath temperature, the effects of heating time at that certain temperature and the effects of changing concentration of silver nitrate on the size of the synthesized nanoparticles was studied. The particle size and morphology of the synthesized silver nanoparticles were identified by SEM analysis. It was found to be 12-200 nm in different parameters (12-25 nm at the best condition) and spherical in shape. It was also found that the size of silver nanoparticles increased with increasing water bath temperature, increasing heating time and increasing silver nitrate concentration. Energy dispersive X-ray spectroscopy was used to confirm that the nanoparticle suspension contains nothing but metallic silver. It was found that 70% of elemental silver nanoparticles were present.

scholarly journals BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Eli Rohaeti ◽  
Endang W Laksono ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

scholarly journals Evaluation of anti bacteria effects of citrate-reduced silver nanoparticles in Staphylococcus aureus and Escherichia coli

2019 ◽  
Vol 10 (4) ◽  
pp. 3636-3643
Author(s):  
Hor Jia Wei ◽  
Mohd. Syafiq Awang ◽  
Nor Dyana ◽  
Daruliza Kernain ◽  
Yazmin Bustami
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Reduction Method ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Vis Spectroscopy ◽  
Mic Value

Silver nanoparticles (AgNPs) has long known for its inhibitory and bactericidal effects. However, due to its’ attractive antibacterial property, on-going research with various synthesis strategies actively been conducted. In this study, the synthesis of AgNPs was reported, using a simple chemical reduction method with citrate as the reducing agent TEM was used to characterize the obtained AgNPs. Then, Staphylococcus aureus and Escherichia coli were used to identify the antibacterial activity of AgNPs. The inhibition effects of AgNPs against these two bacteria were observed via disc diffusion, and MIC assays and the effects of AgNPs mode of action on both bacteria were further observed under TEM. The formation of AgNPs at ̴ 400 nm, which is the surface plasmon resonance peak was observed using Uv-Vis spectroscopy. The size of AgNPs mostly in the range of 1-10 nm and their morphology appeared to be spherical. Based on the MIC assay, Escherichia coli exhibit low MIC value with 0.049 mg/ml as compared to Staphylococcus aureus with0.391 mg/ml MIC value; correspond to the effective antibacterial activity by the citrate-reduced AgNPs. Further observation on the bacterial surface structure can be seen with cross-sectional TEM image, and it provides an insight into the AgNPs mechanistic aspects of AgNPs against Staphylococcus aureus and Escherichia coli. Silver nanoparticles have been successfully synthesised using the citrate reduction method. Results obtained in this study thus elucidating promising findings to employed AgNPs as an antibacterial agent, and this composition needs to be further study and develop into an antibacterial agent.

scholarly journals Synthesis and Characterization of Silver Nanoparticles from Ashyranthus aspera Extract for Antimicrobial Activity Studies

2020 ◽  
Vol 24 (7) ◽  
pp. 1161-1167
Author(s):  
V. Swetha ◽  
S Lavanya ◽  
G. Sabeena ◽  
E. Pushpalaksmi ◽  
Samraj J. Jenson ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Exothermic Reaction ◽  
Visual Observation ◽  
Metallic Silver ◽  
Face Centered Cubic ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Potential Applications

Development of biologically inspired experimental processes for the synthesis of nanoparticles is evolving into an important branch of  nanotechnology. Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we were synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Ashyranthus aspera medicinal plants as bio-reducing agents. UV-Vis spectrometer used to monitor the reduction of Ag ions and the formation of AgNPs in the medium. UV-Vis spectra and visual observation showed that the color of the fresh leaf extracts of Ashyranthus aspera turned into grayish-brown respectively, after treatment with Ag precursors. XRD and SEM have been used to investigate the morphology of prepared AgNPs. The peaks in the XRD pattern are associated with that of the Face-Centered-Cubic (FCC) form of metallic silver. TGA/DTA results associated with weight loss and exothermic reaction due to the desorption of chemisorbed water. FTIR was performed to identify the functional groups which form a layer covering AgNPs and stabilize the AgNPs in the medium. Moreover, silver nanoparticles using aqueous leaf extracts of Ashyranthus aspera were separately tested for their antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Enterobacter). The results showed that the bacterial growth was inhibited by the extracts containing AgNPs Nanoparticles. The biosynthesized nanoparticle was prepared from Ashyranthus aspera leaf extracts exhibits potential applications as broad-spectrum antimicrobial agents Keywords: Ashyranthus aspera, Silver Nanoparticles, Plant extracts, Bacteria, Antibacterial activity.

scholarly journals Biological Activities and Characterization of Silver Nanoparticles from Prunus Prescia (L.) Batsch

2021 ◽  
Vol 10 (3) ◽  
pp. 2466-2482
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Leaf Extract ◽  
Prunus Persica ◽  
Color Change ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Face Centered Cubic ◽  
Plasmon Band ◽  
Vis Spectroscopy

In this paper, Prunus persica L. Batsch (wild and variety) plants have been used for the bioreduction of silver ions to silver nanoparticles. Aqueous leaf extract of plants was treated with silver nitrate. The mixture's color changed from pale yellow to dark brown Prunus persica (variety) and dark yellow to greyish brown Prunus persica (wild). The color change was the first indication of silver nanoparticle synthesis, further confirmed by UV-Vis spectroscopy. The surface Plasmon band exhibited absorption peaks for Prunus persica wild and variety leaf extract at 468 nm, 492 nm, 462 nm. The silver nanoparticles were further characterized using X-Ray Diffraction that carried out the crystallographic nature by exhibiting 2θ value from 10˚ to 90˚ corresponding diffraction planes of Face Centered Cubic structure. Fourier Transform Infrared spectroscopy demonstrated organic compounds in plant material that mainly involved in reduction. Prunus persica L. Batsch (wild and variety) mediated silver nanoparticles exhibited excellent antibacterial activity against a human bacterial pathogen. The antioxidant activity was also studied that showed effective results on synthesized silver nanoparticles using the 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The result showed that Prunus persica L. Batsch proved to be a useful silver nanoparticle.

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