scholarly journals Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 487 ◽  
Author(s):  
Abdalrahim Alahmad ◽  
Armin Feldhoff ◽  
Nadja C. Bigall ◽  
Pascal Rusch ◽  
Thomas Scheper ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Hypericum Perforatum ◽  
Nitrate Solution ◽  
A549 Cells ◽  
Metallic Silver ◽  
Synthesis Methods ◽  
Face Centered Cubic ◽  
Sem Images ◽  
Hypericum Perforatum L

This contribution focuses on the green synthesis of silver nanoparticles (AgNPs) with a size < 100 nm for potential medical applications by using silver nitrate solution and Hypericum Perforatum L. (St John’s wort) aqueous extracts. Various synthesis methods were used and compared with regard to their yield and quality of obtained AgNPs. Monodisperse spherical nanoparticles were generated with a size of approximately 20 to 50 nm as elucidated by different techniques (SEM, TEM). XRD measurements showed that metallic silver was formed and the particles possess a face-centered cubic structure (fcc). SEM images and FTIR spectra revealed that the AgNPs are covered by a protective surface layer composed of organic components originating from the plant extract. Ultraviolet-visible spectroscopy, dynamic light scattering, and zeta potential were also measured for biologically synthesized AgNPs. A potential mechanism of reducing silver ions to silver metal and protecting it in the nanoscale form has been proposed based on the obtained results. Moreover, the AgNPs prepared in the present study have been shown to exhibit a high antioxidant activity for 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation, and super oxide anion radical and 2,2-diphenyl-1-picrylhydrazyl. Synthesized AgNPs showed high cytotoxicity by inhibiting cell viability for Hela, Hep G2, and A549 cells.

scholarly journals Marine plant mediated green synthesis of silver nanoparticles using mangrove Rhizophora stylosa: Effect of variable process and their antibacterial activity

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 768
Author(s):  
Nancy Willian ◽  
Syukri Syukri ◽  
Zulhadjri Zulhadjri ◽  
Syukri Arief
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Narrow Particle Size Distribution ◽  
Metallic Silver ◽  
Face Centered Cubic ◽  
Rhizophora Stylosa ◽  
Vis Spectroscopy

Background: Most natural plants used in the synthesis of silver nanoparticles are limited to marine plants. To carry out applications, colloidal silver nanoparticles (AgNps) should have appropriate properties such as homogeneous shapes, small and narrow particle size distribution, and long time stability. This study aims to determine the effects of a variable process of AgNps mediated mangrove Rhizophora stylosa (RS) leaf extract, and antibacterial activity.  Methods: Synthesis of AgNps was carried out by stirring silver nitrate solution with aqueous extract. The characterization of AgNps was carried out using UV-Vis spectrophotometry, X-ray diffraction (XRD), Dynamic Light Scattering (DLS) zetasizer and Transmission Electron Microscopy (TEM). Evaluation of antibacterial activity was carried out on Escherichia coli and Staphylococcus aureus. Reaction conditions such as the concentration of metal ions (0.001 M, 0.005 M, and 0.01 M), extracts (1%, 3%, and 5% v/v), and the reaction time on the size and stability of nanoparticles were also explored. Results: The UV-Vis spectroscopy showed an absorption of colloidal AgNps in a wavelength range of 403–443 nm.  TEM analysis showed that as-synthesized AgNps were spherical in shape with a size range of 5–87 nm. The use of 0.001 M and 0.005 M of Ag+ resulted in a smaller diameter than the synthesized AgNps, using 0.01 M Ag+, in the same extract concentration. The range of zeta potential was -24.9 mV to -27.7 mV. The as-synthesized AgNps were stable for more than one month. The XRD analysis showed four peaks, which were attributed to the face centered cubic crystal structure of metallic silver. The results of the silver nanoparticles synthesis showed good activity on Escherichia coli and Staphylococcus aureus, with an inhibition zone between 4.1–7.2 mm. Conclusions: The AgNps synthesized with RS leaf extract, which is a reducing agent, showed good potential as an antibacterial component.

Green Synthesis of Silver Nanoparticles by Using Simarouba amara Aubl. Fruit Extract and their Antioxidant and Antibacterial Activities

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Guru Kumar Dugganaboyana ◽  
Chaitra Kyasandra Eranna
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Therapeutic Potential ◽  
Average Particle Size ◽  
Butylated Hydroxytoluene ◽  
Commercial Application ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Fruit Extract ◽  
Sem Images

The green synthesis of nanoparticles has emerged as a cost-effective and environmentally benign technique for therapeutic applications. Nanomedicine utilizes biocompatible nanomaterials for diagnostic and therapeutic potential for various biomedical applications. Different biological methods are gaining recognition over the physical and chemical methods of synthesis for the production of silver nanoparticles (AgNPs) due to their multiple applications. The present study describes the synthesis of AgNPs using the fruit extract of Simaroubaamara (S. amara)followed by characterization of AgNPswas done using different methods, which include; ultraviolet-visible spectroscopy (UV-Vis) wherein it shows absorption peak at 410 nm confirming the AgNPs, from dynamic light scattering (DLS) the average particle size is 80nm with crystalline structure confirmed by scanning electron microscope (SEM) images and zeta potential analysis shows the positive polarity of the particle favoring the drug targeting. The powder X-ray diffraction study (PXRD) revealed crystalline nature with a face-centered cubic (fcc) structure of AgNPs. The synthesized AgNPs were also tested for antioxidant therein the particles could scavenge the stable free radical 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) of about 80% to that of positive control butylated hydroxytoluene (BHT) and antimicrobial studies indicated its microbicidal efficacy against both Gram positive and negative clinical pathogens. It could be concluded that Simaroubaamara fruit extract can be used efficiently in the production of potential antioxidant and antimicrobial AgNPs for commercial application.

scholarly journals Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies

2020 ◽  
Vol 9 (1) ◽  
pp. 283-293
Author(s):  
Milad Torabfam ◽  
Meral Yüce
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Chlorella Vulgaris ◽  
Metallic Nanoparticles ◽  
Nitrate Solution ◽  
Spherical Shape ◽  
Silver Nitrate Solution ◽  
Spectroscopy Analysis ◽  
Bacterial Diseases ◽  
Serovar Typhimurium

AbstractGreen synthesis of metallic nanoparticles (NPs) is acquiring considerable attention due to its environmental and economic superiorities over other methods. This study describes the practical synthesis of silver nanoparticles (AgNPs) through the reduction of silver nitrate solution using an algal source, Chlorella vulgaris, as the reducing as well as the stabilizing agent. The energy required for this synthesis was supplied by microwave radiation. The ultraviolet-visible spectroscopy exhibited a single peak related to the surface plasmon absorbance of AgNPs at 431 nm. The AgNPs with high stability (a zeta potential of −17 mV), hydrodynamic size distribution of 1–50 nm, and mostly spherical shape were obtained through a 10 min process. Fourier transform infrared spectroscopy analysis revealed that several functional groups, including carbonyl groups of C. vulgaris, play a significant role in the formation of functional NPs. Antibacterial features of the produced AgNPs were verified against those of Salmonella enterica subsp. enterica serovar typhimurium and Staphylococcus aureus, demonstrating a considerable growth inhibition at increasing concentrations of the NPs. As a result, the formed AgNPs can be used as a promising agent against bacterial diseases.

Controlling the Surface Plasmon Absorption of Silver Nanoparticles via Green Synthesis Using Pennisetum purpureum Leaf Extract

2018 ◽  
Vol 772 ◽  
pp. 73-77
Author(s):  
Ruelson S. Solidum ◽  
Arnold C. Alguno ◽  
Rey Capangpangan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Surface Plasmon ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Absorption Peak ◽  
Plasmon Absorption ◽  
Surface Plasmon Absorption

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.

scholarly journals Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1777 ◽  
Author(s):  
Md. Mahiuddin ◽  
Prianka Saha ◽  
Bungo Ochiai
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ftir Spectroscopy ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

Green Synthesis of Silver Nanostructures Using Aqueous Extract of Dracocephalum kotschyi and Evaluation of Antioxidant Properties of Herbal Extracts and Antibacterial Feature of Green- Synthesized Nanostructures

2019 ◽  
Vol 13 (3) ◽  
pp. 223-231
Author(s):  
Zahra Goli ◽  
Cobra Izanloo
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Size Distribution ◽  
Aqueous Extract ◽  
Nitrate Solution ◽  
Antioxidant Properties ◽  
Herbal Extract ◽  
Silver Nanostructures ◽  
Dracocephalum Kotschyi ◽  
Vis Spectroscopy

Background: Silver nanoparticles have a profound role in the field of high sensitivity biomolecular detection, catalysis, biosensors and medicine. In the present study, aqueous extract of Dracocephalum kotschyi has been used for the synthesis of silver nanoparticles. Objective: In this study, we evaluated the antioxidant features and the possibility of biosynthesis of AgNPs using an aqueous extract of Dracocephalum kotschyi and also evaluated the antibacterial activities of the synthesized nanoparticles. Methods: An eco-friendly and cost-effective protocol for the synthesis of Ag nanoparticles by utilizing a renewable natural resource, aqueous solution of Dracocephalum kotschyi, was proposed. Synthesized nanoparticles were characterized by UV–Vis spectroscopy, SEM, EDS, and XRD pattern. Results: At first, the extract of Dracocephalum kotschyi was assessed to determine and confirm the presence of an antioxidant feature. Resuscitation of one mM silver nitrate solution was carried out by the herbal extract. The solution containing AgNPs obtained from green synthesis had a maximum optical density at 225 nm. In addition, the presence of AgNPs was approved by energy-dispersive X-ray spectroscopy (EDS). Images of the scanning electron microscope demonstrated that the synthesized AgNPs had the shape of rods and the size distribution of 48-51 nm. One of the benefits of this method is a uniform size distribution. Moreover, the effects of reaction time and concentration of the herbal extract were assessed by ultraviolet-visible (UV-Vis) spectroscopy. In the end, we assessed the antibacterial impact of the synthesized AgNPs against some pathogenic bacterial strains. According to the results, the produced nanostructures had a proper impact on two bacteria of Escherichia coli and Staphylococcus aureus. Conclusion: According to the results of the present study, Dracocephalum kotschyi can be a suitable compound for the synthesis of nanostructures due to its indigenous cultivation and great medicinal properties.

scholarly journals Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Margarita I. Skiba ◽  
Victoria I. Vorobyova ◽  
Alexander Pivovarov ◽  
Natalya P. Makarshenko
Keyword(s):  
Silver Nanoparticles ◽  
Sodium Alginate ◽  
Xrd Analysis ◽  
Face Centered Cubic ◽  
Colloidal Solutions ◽  
Ag Nps ◽  
Sem Images ◽  
Spherical Powder ◽  
Stabilizing Agent ◽  
Vis Spectroscopy

The process of obtaining aqueous solutions of silver nanoparticles with the use of a low-temperature nonequilibrium contact plasma and stabilizing agent—polysaccharide (sodium alginate)—has been examined. The synthesized Ag NPs were characterized by using UV-Vis spectroscopy, dynamic light scattering (DLS), scanning electron microscope (SEM), and XRD analysis. The effect of concentration of Ag+, sodium alginate, duration of processing by plasma discharge, and pH of liquid on the production of silver nanoparticles has been studied. The results demonstrated that synthesis provides the formation of silver nanoparticles for investigated concentrations of Ag+ (0.3-3.0 mmol/l) and 5.0 g/l Na-Alg (pH=7–10) within 1–5 minutes. From the SEM images, the silver nanoparticles are found to be almost spherical. Powder XRD results reveal that Ag nanoparticles have a face-centered cubic crystal structure. Zeta potential of plasma-chemically obtained colloidal solutions at various concentrations of Ag+ ions and stabilizing agent varies from −32.8 to −39.3 mV, indicating the moderate stability of synthesized nanoparticles.

Synthesis of Silver Nanoparticles and their Biomedical Applications - A Comprehensive Review

2019 ◽  
Vol 25 (24) ◽  
pp. 2650-2660 ◽  
Author(s):  
Rajasree Shanmuganathan ◽  
Indira Karuppusamy ◽  
Muthupandian Saravanan ◽  
Harshiny Muthukumar ◽  
Kumar Ponnuchamy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biomedical Applications ◽  
Antibacterial Agents ◽  
Cost Effective ◽  
Metallic Silver ◽  
Synthesis Methods ◽  
Burn Wound ◽  
Chemical Methods ◽  
Biological Sources ◽  
Physical And Chemical

Generally, silver is considered as a noble metal used for treating burn wound infections, open wounds and cuts. However, the emerging nanotechnology has made a remarkable impact by converting metallic silver into silver nanoparticles (AgNPs) for better applications. The advancement in technology has improved the synthesis of NPs using biological method instead of physical and chemical methods. Nonetheless, synthesizing AgNPs using biological sources is ecofriendly and cost effective. Till date, AgNPs are widely used as antibacterial agents; therefore, a novel idea is needed for the successful use of AgNPs as therapeutic agents to uncertain diseases and infections. In biomedicine, AgNPs possess significant advantages due to their physical and chemical versatility. Indeed, the toxicity concerns regarding AgNPs have created the need for non-toxic and ecofriendly approaches to produce AgNPs. The applications of AgNPs in nanogels, nanosolutions, silver based dressings and coating over medical devices are under progress. Still, an improvised version of AgNPs for extended applications in an ecofriendly manner is the need of the hour. Therefore, the present review emphasizes the synthesis methods, modes of action under dissipative conditions and the various biomedical applications of AgNPs in detail.

scholarly journals SYNTHESIS OF SILVER NANOPARTICLES FROM SILVER NITRATE SOLUTION USING GREEN TEA EXTRACT (CAMELIA SINENSIS) AS BIOREDUCTOR

2017 ◽  
Vol 6 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Wara Dyah Pita Rengga ◽  
Arie Yufitasari ◽  
Wismoyo Adi
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Green Tea ◽  
Nitrate Solution ◽  
Green Tea Extract ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Tea Leaves ◽  
Green Tea Leaves ◽  
Tea Extract

The synthesis of silver nanoparticles with micro size is highly required in antibacterial fields. The biorefinery material is highly potential as a bioreductor which is applied in the synthesis of nanoparticles. The bioreductor is made from green tea leaves extraction using aquadest to extract its active substance, the catechin which is derived from polyphenol. The polyphenol can reduce the synthesis of silver nanoparticles naturally. The result of FTIR analysis from green tea leaves extract containing polyphenol shown in the uptake functional groups is -OH group located in 3425 cm-1, C=O group located in 1635 cm-1, C=C group located in 1527, and 1442 cm-1 , and C-O group located in 1234 cm-1. The precursors of AgNO3 was used as the main synthetic material. The synthetic condition was resulted from the reaction between the extraction of green tea extract and AgNO3 as the precursors in the variation of synthetizing time. The heating process during synthesizing is done in 50 ?C along with stirring to foster the creation of silver nanoparticles. The analysis result of XRD shows that silver nanoparticles has the diffraction peaks in the angle of 2 theta that are 44.08, 64.40, and 77.51. The types of silver nanoparticles is Ag0 nanoparticles with face-centered cubic crystal structure. Based on TEM analysis, the size and particle size distribution can be determined using image J. The distribution shows that the longer synthesizing time, the bigger nanoparticles produced. With synthesizing times at 24 hours, 6 hours, 3 hours, and 2 hours produce average particle size of 26.4 nm; 9.2 nm; 8.4 nm; and 7.4 nm respectively.

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