scholarly journals ASSESSMENT OF MINERAL NUTRIENT IMPACT ON METABOLITES ACCUMULATION IN KALE (BRASSICA OLERACEA VAR. SABELLICA)

2021 ◽  
Vol 13 (3) ◽  
pp. 208-224
Author(s):  
Joyce D. Anteh ◽  
Olga A. Timofeeva ◽  
Antonina A. Mostyakova
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Brassica Oleracea ◽  
Phenolic Compound ◽  
Soluble Sugar ◽  
Mineral Nutrients ◽  
Mineral Nutrient ◽  
Health Promoting ◽  
Scientific Attention ◽  
The Impact

Green leafy kale (Brassica oleracea var. sabellica) has huge scientific attention because of its health-promoting functionality. In the present study the impact of NPK, energen, biostim and humate on flavonoid, phenolic compounds, vitamin C, carotenoids, malondialdehyde (MDA), protein, proline and soluble sugar in kale was investigated. The mineral nutrients mostly increased but in some cases maintained the studied metabolites. The stimulatory effect of applied mineral nutrients on the phytochemicals analyzed varied with the different combinations of macro and microelements. Lipid peroxidation was minimized in leaves treated with mineral nutrients hence a reduction in MDA levels. Contrary to the correlation between nitrogen deficiency and increase in polyphenol and vitamin C content in plants, NPK and biostim did not reduce phenolic compound levels. The results of this study showed that NPK maximized the synthesis of vitamin C and proline; energen - phenolic compounds, carotenoids and sugar; biostim – phenolic compounds, proteins and sugar; humate – flavonoids and sugar in curly kale. Therefore, the type of macronutrient and micronutrients combination increases phytochemicals in differently. To enhance the synthesis of phenolic compounds and vitamins, the most promising additives are those containing humic acids (humate and energen), and biostim proved to be more effective for the synthesis of proteins. Background. The understanding of how diet affects the incidence or treatment of disease has led to a rise in consumer’s demand for functional foods as well as created the market for natural sources of health benefitting compounds rather than the synthetic sources. Curly kale has gained scientific attention as a functional food because it contains higher levels of phytochemicals than most vegetables. These phytochemicals have shown antioxidant, antimutagenic, cytotoxic, antifungal, and antiviral activities. However, the content levels of these metabolites are influenced by not only genetic but environmental factors. It was of interest to evaluate how various mineral nutrients can elicit the accumulation of these compounds that minimize the risk of chronic diseases or aid in their treatment. Purpose. Evaluate how the mineral nutrients, NPK, energen, biostim and humate affect the content of metabolites (proteins, sugars, flavonoids, phenolic compounds, vitamin C, carotenoids, MDA and proline) in curly kale (Brassica oleracea var. sabellica). Materials and methods. Sprouts from kale seed kept wet in a Petri dish for 7 days were transferred to the field. At 6 weeks old four mineral nutrients (NPK, energen, humate and biostim) were added to the soil. Control variants were treated with water. A week later, the leaves were harvested after which, the phenolic compound, flavonoid, protein, sugar, vitamin C, carotenoid, MDA and proline contents were determined using spectrophotometric methods. Results. It was shown that humate fertilizer elicited the highest accumulation of flavonoids. Kale plants fertilized with energen were observed to have the highest phenolic compound content. NPK, energen and humate caused a similarly positive effect on vitamin C content in leaves, unlike biostim whose effect did not significantly differ from control plants. Energen treated kale had the highest increment of carotenoids. A varied reduction of MDA levels in plants treated with all four mineral nutrients was observed in kale leaves. Plants fertilized with biostim accrued the highest protein content in leaves. Proline content increased under the influence of all fertilizers studied. Sugar levels for all kale plants treated with the studied mineral nutrients were enhanced equally Conclusion. Macro and microelements supplied by mineral nutrients differentially boost the biosynthesis of health-promoting metabolites in curly kale, thereby enhancing its quality.

scholarly journals Effects of humate and neodymium on phytochemical levels in kale at different ontogenetic stages

2021 ◽  
Vol 12 (4) ◽  
pp. 740-744
Author(s):  
J. D. Anteh ◽  
O. A. Timofeeva ◽  
A. A. Mostyakova
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Brassica Oleracea ◽  
Chronic Diseases ◽  
Mineral Nutrients ◽  
Spectrophotometric Analysis ◽  
Mineral Nutrient ◽  
Growth Stages ◽  
Potassium Humate ◽  
Phytochemical Content

The increased global mortality caused by the rise in chronic diseases can be reduced by the consumption of antioxidant-rich foods. Leafy green kale (Brassica oleracea var. sabellica) has high nutritional value due to its high antioxidant content and its consumption is highly correlated with a reduced risk of developing some chronic diseases. The paper focuses on examining the effects of two different mineral nutrients – neodymium and potassium humate – on the accumulation of flavonoids, vitamin C, phenolic compounds, carotenoids and malondialdehyde (MDA) content in field-grown leafy green kale. Leaves from treated plants were collected at three stages of maturity –14 weeks, 18 weeks, and 22 weeks and subjected to spectrophotometric analysis. The results showed that the stimulatory effect of both mineral nutrients on the phytochemicals varied at the different growth stages - the highest significant effect of neodymium has been mostly observed at week 18 with high flavonoid, vitamin C, and carotenoid levels. Phenolic compounds for this variant are the same as in the control samples, while the level of malondialdehyde was reduced by 21.8%, signifying increased antioxidant activity. The rare earth element primarily maintained some phytochemical content at weeks 14 and 22. Following soil treatment with potassium humate, the levels of studied phytochemical compounds were either maintained or elevated at weeks 14, 18, and 22. Humic acid exerted the greatest decrease in malondialdehyde content in kale at week 14, indicating a reduction in the lipid peroxidation process in leaves. Accordingly, the harvest date of leafy green kale (Brassica oleracea var. sabellica) should depend on the type of mineral nutrient applied. The obtained results provide information that may be relevant to the production of functional varieties and enhance the nutritional and possibly the economic value of kale.

scholarly journals Effect of potassium humate and growth stage on phenolic compound and vitamin C accumulation in kale (Brassica oleracea var. sabellica)

2021 ◽  
Vol 941 (1) ◽  
pp. 012032
Author(s):  
J D Anteh ◽  
O A Timofeeva ◽  
A A Mostyakova
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Brassica Oleracea ◽  
Phenolic Compound ◽  
Antioxidant Properties ◽  
Growth Stages ◽  
Potassium Humate ◽  
Antioxidant Profile ◽  
Brassica Crops ◽  
Different Growth Stages

Abstract Kale is one of the top economically valuable crops in the world because of its high antioxidant content. Research shows that the antioxidant profile of Brassica crops varies with growth stages due to soil fertility, temperature, light and other agronomic factors. This study aimed to analyze the effect of potassium humate on phenolic compound content, the greatest contributor to the antioxidant properties of the highest-ranking superfood kale (Brassica oleracea var. sabellica). Our results showed that potassium humate at different growth stages elicited phenolic compounds in the studied samples. Leaves of 22 weeks old plants accumulated phenolic compounds about two times higher than those of 7 weeks old plants. Vitamin C content was increased by potassium humate treatment at 7 weeks. At 22 weeks levels in controls and treated kale leaves did not significantly differ.

scholarly journals Effect of mineral fertilizers on the variability of phytochemicals in kale

2021 ◽  
Vol 1 (43) ◽  
pp. 8-8
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Antioxidant Activity ◽  
Trace Elements ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Mineral Nutrients ◽  
Mineral Fertilizers ◽  
Maturity Stage ◽  
Biochemical Compounds ◽  
Brassica Vegetables ◽  
Total Antioxidant

The effect of five mineral nutrients on the variability of the content of biochemical compounds, such as flavonoids, phenolic compounds, vitamin C, carotenoids and the antioxidant activity of kale (Brassica Oleracea var. sabellica) collected at the edible maturity stage. Manipulation of the phytochemical composition of the kale cabbage using mineral fertilizers was evident in the results achieved. The various mineral nutrients used had different effects on the content of the studied phytochemicals. The content of flavonoids and carotenoids did not decrease with any of the various mineral nutrients used. Contrary to recent research suggesting that rare earth elements are good stimulators of secondary metabolite accumulation, our results showed a decrease in the content of phenolic compounds in plants fertilized with neodymium. The total antioxidant activity increased under the influence of NPK and humate, which correlated with the highest content of soluble phenolic compounds and flavonoids in kale leaves. Low doses of N have a positive effect on these indicators. Mineral supplements of trace elements also had a stimulating effect on the accumulation of the studied substances. However, in addition to trace elements, a significant role is played by the presence of humic acids (in humate), which together with trace elements have the maximum stimulating effect on the content of the substances under study. Keywords: MINERAL FERTILIZERS, ANTIOXIDANT PHYTOCHEMICALS, PHENOLIC COMPOUNDS, CAROTENOID, ANTIOXIDATIVE ACTIVITY, VITAMIN C, FLAVONOIDS, BRASSICA VEGETABLES

scholarly journals Main Health-Promoting Compounds Response to Long-Term Freezer Storage and Different Thawing Methods in Frozen Broccoli Florets

Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 375
Author(s):  
Miao ◽  
Lin ◽  
Zeng ◽  
Wang ◽  
Yao ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Total Phenols ◽  
Freezer Storage ◽  
Health Promoting ◽  
Nutritional Compounds ◽  
Better Than

The effects of long-term freezer storage and different defrosting methods on the retention of glucosinolates, vitamin C, and total phenols in frozen broccoli florets were investigated in the present study. Frozen broccoli florets were stored in a freezer at −20 °C for 165 days or subjected to defrosting by three different house-hold thawing methods (water, air, and refrigerator defrosting). Results showed that all glucosinolates were well preserved, while vitamin C and total phenols were reduced by less than 12% and 19% of the control, respectively, during the storage. Besides, refrigerator and air defrosting were better than water defrosting in glucosinolates retention, and refrigerator defrosting was the best in vitamin C preservation. No difference was observed in reserving phenolic compounds among the three methods. In conclusion, long-term freezer storage is an excellent way to preserve broccoli florets, and refrigerator defrosting is the best way to maintain the nutritional compounds in frozen broccoli florets.

scholarly journals Daytime, Not Nighttime, Elevated Atmospheric Carbon Dioxide Exposure Improves Plant Growth and Leaf Quality of Mulberry (Morus alba L.) Seedlings

2021 ◽  
Vol 11 ◽  
Author(s):  
Songmei Shi ◽  
Yuling Qiu ◽  
Miao Wen ◽  
Xiao Xu ◽  
Xingshui Dong ◽  
...  
Keyword(s):  
Plant Growth ◽  
Atmospheric Carbon Dioxide ◽  
Plant Biomass ◽  
Soluble Sugar ◽  
Morus Alba ◽  
Mineral Nutrient ◽  
Leaf Quality ◽  
Leaf Yield ◽  
The Impact

Almost all elevated atmospheric CO2 concentrations (eCO2) studies have not addressed the potential responses of plant growth to different CO2 in daytime and nighttime. The present study was to determine the impact of daytime and/or nighttime eCO2 on growth and quality of mulberry (Morus alba L.), a perennial multipurpose cash plant. Six-month-old mulberry seedlings were hence grown in environmentally auto-controlled growth chambers under four CO2 concentrations: (1) ambient CO2 (ACO2, 410 μmol mol–1 daytime/460 μmol mol–1 nighttime), (2) sole daytime elevated CO2 (DeCO2, 710 μmol mol–1/460 μmol mol–1), (3) sole nighttime elevated CO2 (NeCO2, 410 μmol mol–1/760 μmol mol–1), and (4) continuous daytime and nighttime elevated CO2 (D + NeCO2, 710 μmol mol–1/760 μmol mol–1). Plant growth characteristics, nutrient uptake, and leaf quality were then examined after 120 days of CO2 exposure. Compared to control, DeCO2 and (D + N)eCO2 increased plant biomass production and thus the harvest of nutrients and accumulation of leaf carbohydrates (starch, soluble sugar, and fatty acid) and N-containing compounds (free amino acid and protein), though there were some decreases in the concentration of leaf N, P, Mg, Fe, and Zn. NeCO2 had no significant effects on leaf yield but an extent positive effect on leaf nutritional quality due to their concentration increase in leaf B, Cu, starch, and soluble sugar. Meanwhile, (D + N)eCO2 decreased mulberry leaf yield and harvest of nutritious compounds for silkworm when compared with DeCO2. The reason may be associated to N, P, Mg, Fe, and Zn that are closely related to leaf pigment and N metabolism. Therefore, the rational application of mineral nutrient (especially N, P, Fe, Mg, and Zn) fertilizers is important for a sustainable mulberry production under future atmosphere CO2 concentrations.

scholarly journals The Impact of Domestic Cooking Methods on Myrosinase Stability, Glucosinolates and Their Hydrolysis Products in Different Cabbage (Brassica oleracea) Accessions

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2908
Author(s):  
Omobolanle O. Oloyede ◽  
Carol Wagstaff ◽  
Lisa Methven
Keyword(s):  
Brassica Oleracea ◽  
Residual Activity ◽  
Cooking Methods ◽  
Hydrolysis Products ◽  
Brassica Vegetables ◽  
Glucosinolate Concentration ◽  
Health Promoting ◽  
The Impact ◽  
Myrosinase Activity

Glucosinolate hydrolysis products are responsible for the health-promoting properties of Brassica vegetables. The impact of domestic cooking on the myrosinase stability, glucosinolates and hydrolysis products in 18 cabbage accession was investigated. Cabbages were steamed, microwaved, and stir-fried before analysis. Cooking significantly affected myrosinase stability and glucosinolate concentrations within and between cabbage morphotypes. Myrosinase was most stable after stir-frying, with up to 65% residual activity. Steaming and microwaving resulted in over 90% loss of myrosinase activity in some accessions. Stir-frying resulted in the greatest decrease in glucosinolate concentration, resulting in up to 70% loss. Steamed cabbages retained the highest glucosinolates after cooking (up to 97%). The profile and abundance of glucosinolate hydrolysis products detected varied across all cooking methods studied. Cooking reduced the amounts of nitriles and epithionitriles formed compared to raw samples. Steaming led to a significant increase in the concentration of beneficial isothiocyanates present in the cabbage and a significantly lower level of nitriles compared to other samples. Microwaving led to a reduction in the concentrations of both nitriles and isothiocyanates when compared to other cooking methods and raw cabbage. The results obtained help provide information on the optimal cooking methods for cabbage, suggesting that steaming may be the best approach to maximising beneficial isothiocyanate production.

scholarly journals Transient Nutrient Deficiencies in Pea: Consequences on Nutrient Uptake, Remobilization, and Seed Quality

2021 ◽  
Vol 12 ◽  
Author(s):  
Cécile Jacques ◽  
Marion Forest ◽  
Vincent Durey ◽  
Christophe Salon ◽  
Alain Ourry ◽  
...  
Keyword(s):  
Seed Quality ◽  
Plant Biomass ◽  
Negative Impact ◽  
Nutrient Deficiency ◽  
Dry Weight ◽  
Nutrient Content ◽  
Mineral Nutrients ◽  
Mineral Nutrient ◽  
Nutrient Deficiencies ◽  
The Impact

Legume plants, such as peas, are of significant nutritional interest for both humans and animals. However, plant nutrition and thus, seed composition, depends on soil mineral nutrient availability. Understanding the impact of their deprivation on the plant mineral nutrient content, net uptake, and remobilization is of key importance but remains complex as the elements of the plant ionome are linked in intricate networks, one element deprivation impacting uptake and remobilization of other nutrients. To get a better insight into pea mineral nutrition, the transitory deprivations of 13 mineral nutrients were imposed during the vegetative growth phase. Thereafter, plants were grown under optimal mineral conditions until physiological maturity. Plant nutritional status and seed quality impacts caused by the deprivations were characterized using measurement of mineral nutrient concentration and plant biomass allocation. Our results highlight: (i) the preferential allocation of dry weight and elements to shoots at the expense of the roots under non-limiting conditions, and more particularly to the tendrils in comparison to the other shoot organs, (ii) the positive and/or negative impact of one mineral nutrient deprivation on other elements of the ionome, (iii) four different remobilization strategies for eight mineral nutrients, and (iv) possible strategies to improve seed quality via fine control of fertilization during a period of mineral nutrient deficiency.

scholarly journals Developing an Olive Biorefinery in Slovenia: Analysis of Phenolic Compounds Found in Olive Mill Pomace and Wastewater

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 7
Author(s):  
Ana Miklavčič Višnjevec ◽  
Paul Baker ◽  
Adam Charlton ◽  
Dave Preskett ◽  
Kelly Peeters ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Phenolic Content ◽  
Seasonal Effects ◽  
Total Phenolic ◽  
Array Detector ◽  
Economic Modelling ◽  
Olive Pomace ◽  
The Impact ◽  
Olive Mill

The valorization of olive pomace through the extraction of phenolic compounds at an industrial scale is influenced by several factors that can have a significant impact on the feasibility of this approach. These include the types and levels of phenolic compounds that are present, the impact that seasonal variation and cultivar type have on the phenolic compound content in both olive pomace and mill effluents and the technological approach used to process the olive crop. Chemical analysis of phenolic compounds was performed using an HPLC-diode-array detector (DAD)-qTOF system, resulting in the identification of 45 compounds in olive mill wastewater and pomace, where secoiridoids comprised 50–60% of the total phenolic content. This study examined three different factors that could impact the phenolic compound content of these processing streams, including cultivar types typically grown on local farms in Slovenia, the type of downstream processing used and seasonality effects. Olive crop varieties sourced from local farms showed high variability, and the highest phenolic content was associated with the local variety “Istrska Belica”. During processing, the phenolic content was on average approximately 50% higher during two-phase decanting compared to three-phase decanting and the type of compound present significantly different. An investigation into the seasonal effects revealed that the phenolic content was 20% higher during the 2019 growing season compared to 2018. A larger sample size over additional growing seasons is required to fully understand the annual variation in phenolic compound content. The methods and results used in this study provide a basis for further analysis of phenolic compounds present in the European Union’s olive crop processing residues and will inform techno-economic modelling for the development of olive biorefineries in Slovenia.

scholarly journals Phenolics and Mineral Elements Composition in Underutilized Apple Varieties

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 40
Author(s):  
Alessandra Francini ◽  
Carmen Fidalgo-Illesca ◽  
Andrea Raffaelli ◽  
Luca Sebastiani
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Mineral Elements ◽  
Mineral Nutrients ◽  
Apple Varieties ◽  
Commercial Cultivars ◽  
Golden Delicious ◽  
Phenolic Phytochemicals ◽  
Excellent Source

(1) Background: The aim of this research is to study the importance of improving knowledge of old variety apples in terms of phytochemicals, antioxidant capacity and mineral elements. (2) Methods: Fifteen phenolic compounds (UHPLC-MS/MS techniques), eight mineral elements (Ca, K, Cu, Mn, Fe, Zn, Na and Mg), antioxidant capacity (DPPH%) and vitamin C content in four ancient apple varieties of Tuscany (‘Mora’, ‘Nesta’, ‘Panaia’ and ‘Ruggina’), with the ‘Golden Delicious’ cultivar as reference, were analyzed. (3) Results: Ancient cultivars exhibited a superior antioxidant capacity compared to commercial cultivars. ECTC and CGA were the polyphenols that mostly characterized the ancient apples. The contents of the elements of concern, Zn and Mn, were higher in the ancient cultivars ‘Panaia’ and ‘Ruggina’, while Na showed significant low concentration in ‘Nesta’, ‘Panaia’ and ‘Ruggina’ compared to ‘Golden Delicious’. The vitamin C content also indicated that ‘Mora’, ‘Panaia’ and ‘Ruggina’ old variety had an AsA content around 10 times higher than the ‘Golden Delicious’ apple. (4) Conclusions: Underutilized varieties could be an excellent source of bioactive phenolic phytochemicals, mineral nutrients and vitamins that may offer special nutraceutical benefits compared to other fruits.

scholarly journals Bioactive compounds and antioxidant activity in tomatoes (Lycopersicon esculentum L.) cultivars in natura and after thermal processing

2020 ◽  
Vol 9 (11) ◽  
pp. e44991110192
Author(s):  
Angela Vacaro de Souza ◽  
Jéssica Marques de Mello ◽  
Vitória Ferreira da Silva Fávaro ◽  
Victória Farias da Silva ◽  
Diogo de Lucca Sartori ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Lycopersicon Esculentum ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Thermal Processing ◽  
Antioxidant Activities ◽  
The Impact ◽  
Β Carotene

The aim of the study was to evaluate the impact of processing by cooking in natura fruits on the content of bioactive compounds - vitamin C and ascorbic acid, phenolic compounds, flavonoids, carotenoids and anthocyanins, lycopene and β-carotene - and on antioxidant activity - DPPH and FRAP - of 9 varieties of commercial and non-commercial tomatoes. The fruits were harvested when they reached the point of physiological maturation, selected and evaluated in natura, and after grinding and cooking for 30 minutes. At the end of the experiment it was found that all bioactive compounds analyzed showed quantitative reductions when the fruits were processed, with cherry cultivars - 7, 8 and 9 - those that showed superior results compared to different bioactive compounds evaluated, proving to be interesting to be better explored. Regarding the antioxidant activities, the processed fruits of these cultivars showed less losses showing potential to be submitted to processing.

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