scholarly journals Histopathological observation and health status of the zebra-snout seahorse Hippocampus barbouri Jordan & Richardson, 1908 in captivity

2021 ◽  
Vol 19 (3) ◽  
pp. 305-315
Author(s):  
Thatpon Kamnurdnin ◽  
◽  
Sinlapachai Senarat ◽  
Jes Kettratad ◽  
Supanut Pairohakul ◽  
...  
Keyword(s):  
Health Status ◽  
Hepatic Tissue ◽  
Renal Tubules ◽  
Adult Fish ◽  
Histological Techniques ◽  
Histopathological Alterations ◽  
In Captivity ◽  
Liver And Kidney ◽  
Improved Methods ◽  
Trematode Parasites

The health status of the zebra-snout seahorse, Hippocampus barbouri in captivity has been required for approval for aquaculture. In this study, we investigated the histopathological appearance of three vital organs including gill, kidney and liver in captive H. barbouri during its juvenile and adult stages, by using histological techniques. In juveniles from stage 14-days (100% prevalence) towards stage 30-days adults (100% prevalence), the gills exhibited intraepithelial edema and necrosis while hepatic tissue showed evidence of intracytoplasmic vacuoles. In addition, histological alteration to renal tissues was observed the degeneration of renal tubules, the presence of melanomacrophage, and the infection of trematode parasites. The parasites were found in stage 30-days adult fish in the kidney (33.3 % prevalence). Taken together, this study highlights the issue of health in captive rearing of H. barbouri, in particular histopathological alterations in gill, liver and kidney tissues, suggesting that aquaculture of this seahorse species requires improved methods and protocols for maintenance and preventing infection.

scholarly journals Investigation on culture and health status of Mrigal Cirrhinus cirrhosus from the farming system of Mymensingh region

2014 ◽  
Vol 11 (2) ◽  
pp. 391-397
Author(s):  
GU Ahmed ◽  
K Konica ◽  
MF Ali ◽  
T Khatun
Keyword(s):  
Health Status ◽  
Pathological Condition ◽  
Farming System ◽  
Health Condition ◽  
Fish Farms ◽  
Histological Techniques ◽  
Liver And Kidney ◽  
Gill Filaments ◽  
Culture And Health ◽  
Histopathological Studies

Indian major carp, Cirrhinus cirrhosus was considered to monitor culture and health status in farm condition from Bhaluka and Muktagacha of Mymensingh region of Bangladesh for a period of seven months starting from September, 2010 to March, 2011. Monthly sampling was carried out for growth and health condition. Samples of skin and muscle, gill, liver and kidney were observed by clinical and histological techniques. Water temperature was at unfavorable levels but, dissolved oxygen, pH, alkalinity, ammonia and nitrite of water were within the favorable range for fish culture during the months of December and January. Clinically weak body, rough skin, scale loss, thick gill filaments, ulcer and gray brownish color were observed. Among the affected fish organs like skin, muscle and gill were found to be more affected than those of the internal organs, liver and kidney. Major pathologies in the skin and muscle were loss of epidermis and dermis, presence of fungal granuloma, fungal hyphae, necrosis, hemorrhages, vacuums and melanocytes. Protozoan cysts, clubbing, hemorrhages, necrosis and hypertrophy were found in the gill. Necrosis, hemorrhage, pyknosis and vacuums were observed in liver. Common pathological signs like necrosis, hemorrhage, pyknosis, vacuums and degenerated kidney tubules were noticed in kidney. Overall, in the clinical and histopathological studies fishes were found to be more affected during the colder months i.e., December and January. Whereas, in the month of February the pathological condition of fish gradually started to heal up. In histopathological observation, fishes of Bhaluka farms were more affected than that of Muktagacha fish farms. Epizootic ulcerative syndrome (EUS) and protozoan diseases were noticed in Bhaluka fish farms. DOI: http://dx.doi.org/10.3329/jbau.v11i2.19947 J. Bangladesh Agril. Univ. 11(2): 391-397, 2013

scholarly journals Assessment of the Protective Effect of Lepidium sativum against Aluminum-Induced Liver and Kidney Effects in Albino Rat

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Maha Jameal Balgoon
Keyword(s):  
Structural Changes ◽  
Lepidium Sativum ◽  
Albino Rats ◽  
Renal Tubules ◽  
Albino Rat ◽  
Antioxidant Power ◽  
Superior Effect ◽  
Ferric Reducing Antioxidant Power ◽  
Liver And Kidney ◽  
Induced Changes

Background and Objectives. Environmental pollution with the different Aluminum (Al) containing compounds has been increased. Liver and kidney are two vital organs targeted by Al accumulation. The aim of this study was to assess the possible protective and curative effects of Lepidium sativum Linn (LS) against Al-induced impairment of liver and kidney in albino rat and to explore the mechanism behind this effect. Materials and Methods. This experimental animal-based study included fifty albino rats divided into five groups, the control, LS-treated (20 mg/kg), AlCl3-treated (10 mg/kg), AlCl3 then LS, and AlCl3 plus LS-treated, simultaneously for 8 weeks. At the end of the experiment, hepatic and renal functions as well as the biomarkers of antioxidants activities were assessed in the serum. Both liver and kidney were dissected out and histopathologically examined. Results. This study showed that administration of AlCl3 caused a significant (p<0.05) reduction in rats body weight. It significantly increased serum AST, ALT, ALP, bilirubin, urea, and creatinine levels and decreased total protein and albumin. AlCl3 significantly reduced enzymatic (catalase), nonenzymatic (reduced glutathione), and ferric reducing antioxidant power (FRAP) in the serum. Histopathologically, it induced necrosis and degeneration of hepatocytes, glomeruli, and renal tubules. Administration of LS after or along with AlCl3 significantly restored the serum biomarkers of liver and kidney functions to their near-normal levels and had the ability to overcome Al-induced oxidative stress and preserved, to some extent, the normal hepatic and renal structure. The coadministration of LS had a superior effect in alleviating Al-induced changes. Conclusion. Exposure to AlCl3 induced a set of functional and structural changes in the liver and kidney of rats evident through both biochemical and histopathological assessment. The antioxidant activity of LS seeds mediated a protective and curative effect of LS against such changes. Further study through a rigorous clinical trial to prove LS activity on human is recommended.

scholarly journals Agave americana (Ketuke) toxicity in the kidney of Clarias batrachus

2018 ◽  
Vol 30 ◽  
pp. 244-247
Author(s):  
Archana Prasad ◽  
Rukmini Puri
Keyword(s):  
Behavioral Change ◽  
The Other ◽  
Clarias Batrachus ◽  
Epithelial Tissue ◽  
Renal Tubules ◽  
Histopathological Alterations ◽  
Agave Americana ◽  
Excessive Secretion ◽  
Loss Of Appetite ◽  
Different Parts

Different parts of plant have been used since many decades by humans for catching fishes. In this investigation, ‘ketuke ‘leaf effect was observed on behavioral change and histological alterations in the kidney of Clarias batrachus. Few fishes were kept as control i.e. not exposed to plant piscicide. The other fishes were exposed to 3gm per 30 liters of water for 24 and 48 hrs. In exposed fish’s restlessness, vigorous movement, excessive secretion of slime and loss of appetite was seen. Histologically, glomerular contraction, clumping of melanomacrophages, degeneration of epithelial tissue along with the degeneration of renal tubules and others were seen. Increase in the Bowman’s space was one of the noticeable changes. Extract accumulation was seen in the tissues. Exposure to sub lethal concentrations of Ketuke (Agave americana), caused duration dependent histopathological alterations in the kidney of Clarias batrachus which is discussed in the paper.

Ferroportin expression in haem oxygenase 1-deficient mice

2012 ◽  
Vol 449 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Rafał R. Starzyński ◽  
François Canonne-Hergaux ◽  
Małgorzata Lenartowicz ◽  
Wojciech Krzeptowski ◽  
Alexandra Willemetz ◽  
...  
Keyword(s):  
Culture Medium ◽  
Basolateral Membrane ◽  
Renal Tubules ◽  
Leukaemia Virus ◽  
Hepcidin Expression ◽  
Systemic Factors ◽  
Liver And Kidney ◽  
Feline Leukaemia Virus ◽  
Haem Oxygenase ◽  
Deficient Mice

HO1 (haem oxygenase 1) and Fpn (ferroportin) are key proteins for iron recycling from senescent red blood cells and therefore play a major role in controlling the bioavailability of iron for erythropoiesis. Although important aspects of iron metabolism in HO1-deficient (Hmox1−/−) mice have already been revealed, little is known about the regulation of Fpn expression and its role in HO1 deficiency. In the present study, we characterize the cellular and systemic factors influencing Fpn expression in Hmox1−/− bone marrow-derived macrophages and in the liver and kidney of Hmox1−/− mice. In Hmox1−/− macrophages, Fpn protein was relatively highly expressed under high levels of hepcidin in culture medium. Similarly, despite high hepatic hepcidin expression, Fpn is still detected in Kupffer cells and is also markedly enhanced at the basolateral membrane of the renal tubules of Hmox1−/− mice. Through the activity of highly expressed Fpn, epithelial cells of the renal tubules probably take over the function of impaired system of tissue macrophages in recycling iron accumulated in the kidney. Moreover, although we have found increased expression of FLVCR (feline leukaemia virus subgroup C receptor), a haem exporter, in the kidneys of Hmox1−/− mice, haem level was increased in these organs. Furthermore, we show that iron/haem-mediated toxicity are responsible for renal injury documented in the kidneys of Hmox1−/− mice.

Histopathological alterations in gill, liver and kidney of common carp exposed to chlorpyrifos

2012 ◽  
Vol 47 (3) ◽  
pp. 180-195 ◽  
Author(s):  
Sandipan Pal ◽  
Emiko Kokushi ◽  
Jiro Koyama ◽  
Seiichi Uno ◽  
Apurba R. Ghosh
Keyword(s):  
Common Carp ◽  
Histopathological Alterations ◽  
Liver And Kidney

PROPERTIES AND CLASSIFICATION OF THE SOLUBLE ESTERASES OF HUMAN KIDNEY

1964 ◽  
Vol 42 (2) ◽  
pp. 277-286 ◽  
Author(s):  
D. J. Ecobichon ◽  
W. Kalow
Keyword(s):  
Human Kidney ◽  
Lactic Dehydrogenase ◽  
Renal Tissue ◽  
Water Soluble ◽  
Serum Cholinesterase ◽  
Hepatic Tissue ◽  
Liver And Kidney ◽  
Starch Gel ◽  
Zone Characteristic

Water-soluble proteins and enzymes of human kidney were separated by vertical zone electrophoresis in starch gel and compared with those of human serum and liver. In most individuals 11 bands of proteins were detected with the aid of amido black B; some individuals had one additional band. Various substrates and inhibitors were used in efforts to identify enzymes. Five zones of esterase activity were found. One zone, characteristic of serum cholinesterase, was believed to be due to serum contained in the tissue. A zone of isozymic esterases was found to be common to both human liver and kidney and reacted like acetylesterase. Another zone, migrating at a rate approximating that of serum albumin, reacted like an aliesterase. Three small esterase bands, showing a marked hydrolysis of α-naphthyl butyrate, were found to be characteristic of renal tissue on comparison with hepatic tissue and serum. Observations on alkaline phosphatase, acid phosphatase, leucine aminopeptidase, lactic dehydrogenase, and catalase were recorded for comparison with the data on esterases.

scholarly journals Haematological Parameters and Histomorphological Effect of Varied Parts of Citrullus Lanatus Juice Fed to Adult Female Mice

2021 ◽  
Vol 24 (11) ◽  
pp. 1955-1961
Author(s):  
OA Iwalaye ◽  
VO Ekundina ◽  
A Oni
Keyword(s):  
Negative Impact ◽  
Citrullus Lanatus ◽  
Antioxidant Properties ◽  
Kidney Tissue ◽  
Control Group ◽  
Renal Tubules ◽  
Water Control ◽  
Female Mice ◽  
Liver And Kidney ◽  
Histopathological Effects

Plants basically will continue to be the starting materials in making modern drugs. As a result of this, many believe in using herbal products than convectional drugs because of their easy accessibility, low side effects and affordability. Citrulluslanatus (popularly known as watermelon) is a citrus fruit known to contain among others, citrulline and lycopene with hepatoprotective actions on organs such as liver and kidney because of it bioactive and antioxidant properties. This study investigates the haemathological parameters and histopathological effects of crude C. lanatus juice on the liver and kidney of mice. Twenty five female mice weighing between 72 - 99g were randomly divided into five groups (A - E) of five mice each. Group A, were given water (control); group B, were given 2ml of watermelon flesh juice; group C, were given 2ml of watermelon flesh and seed juice, group D, were given 2ml of watermelon flesh and rind juice; and group E were given 2ml ofwatermelon flesh, seed and rind juice once daily for four weeks. At the end of experiment, animals were sacrificed and dissected. Blood sample were taken through cardiac puncture, liver and kidney tissue were excised also to determine the haematological and histopathological effects using routine diagnostic techniques. Highest blood and differential counts except WBC were obtained in groups exposed to crude C. lanatus juice when compared with the control.The liver of animals in all groups appears normal with no remarkable differences from the control. Also, no remarkable differences were recorded in the kidney of animals in all groups except group E which showed interstitial edema with some renal tubules within the cortex undergoing degeneration. From this study and at the dose and duration of study, every part of C. lanatushad no negative impact on the histopathology of the liver and kidney and also had the potential to boost the immune system. Keywords: Citrullus lanatus, haematology, histopathology, kidney, liver, mice

scholarly journals Acetaminophen Induced Oxidative and Histopathological Alterations in Hepatic Tissue: Protective Effects of Alstonia Scholaris Leaf Extracts

2016 ◽  
Vol 8 (4) ◽  
pp. 385-391 ◽  
Author(s):  
Pawan Kumar Verma ◽  
Rajinder Raina ◽  
Mudasir Sultana ◽  
Maninder Singh ◽  
Pawan Kumar
Keyword(s):  
Hepatic Tissue ◽  
Protective Effects ◽  
Leaf Extracts ◽  
Histopathological Alterations ◽  
Alstonia Scholaris

scholarly journals The multiple organs insult and compensation mechanism in mice exposed to hypobaric hypoxia

2020 ◽  
Vol 25 (5) ◽  
pp. 779-791 ◽  
Author(s):  
Ning Li ◽  
Qiuyue Li ◽  
Jinrong Bai ◽  
Ke Chen ◽  
Hailing Yang ◽  
...  
Keyword(s):  
Hypoxia Inducible Factor ◽  
Inflammatory Cells ◽  
Feed Consumption ◽  
Renal Tubules ◽  
Compensation Mechanism ◽  
Basal Cells ◽  
Multiple Organs ◽  
Liver And Kidney ◽  
Endothelial Growth Factor ◽  
The Brain

Abstract This study was first and systematically conducted to evaluate the hypoxia response of the brain, heart, lung, liver, and kidney of mice exposed to an animal hypobaric chamber. First, we examined the pathological damage of the above tissues by Hematoxylin & eosin (H&E) staining. Secondly, biochemical assays were used to detect oxidative stress indicators such as superoxide dismutase (SOD), malondialdehyde (MDA), reduced glutathione (GSH), and oxidized glutathione (GSSG). Finally, the hypoxia compensation mechanism of tissues was evaluated by expression levels of hypoxia-inducible factor 1 alpha (HIF-1α), erythropoietin (EPO), and vascular endothelial growth factor (VEGF). During the experiment, the mice lost weight gradually on the first 3 days, and then, the weight loss tended to remain stable, and feed consumption showed the inverse trend. H&E staining results showed that there were sparse and atrophic neurons and dissolved chromatin in the hypoxia group. And hyperemia occurred in the myocardium, lung, liver, and kidney. Meanwhile, hypoxia stimulated the enlargement of myocardial space, the infiltration of inflammatory cells in lung tissue, the swelling of epithelial cells in hepatic lobules and renal tubules, and the separation of basal cells. Moreover, hypoxia markedly inhibited the activity of SOD and GSH and exacerbated the levels of MDA and GSSG in the serum and five organs. In addition, hypoxia induced the expression of HIF-1α, EPO, and VEGF in five organs. These results suggest hypoxia leads to oxidative damage and compensation mechanism of the brain, heart, lung, liver, and kidney in varying degrees of mice.

Adverse effects of lactational exposure to chlorpyrifos in suckling rats

2009 ◽  
Vol 29 (2) ◽  
pp. 77-92 ◽  
Author(s):  
SA Mansour ◽  
AH Mossa
Keyword(s):  
Body Weight ◽  
Adverse Effects ◽  
Body Weight Gain ◽  
Lethal Dose ◽  
Daily Intake ◽  
Oral Route ◽  
The Body ◽  
Lactational Exposure ◽  
Histopathological Alterations ◽  
Liver And Kidney

The present study was undertaken to evaluate the oxidative damage, biochemical and histopathological alterations in sucking rats whose mothers were exposed to the insecticide chlorpyrifos (CPF). Dams were administered CPF, via oral route. Doses equalled 0.01 mg kg—1 body weight (b.wt.; acceptable daily intake, ADI), 1.00 mg kg—1 b.wt. (no observed adverse effects level, NOAEL) and 1.35 mg kg—1 b.wt. (1/100 lethal dose [LD50]) from postnatal day 1 until day 20 after delivery. At two high doses of CPF, the body weight gain and relative liver and kidney weight of suckling pups were significantly decreased. Exposure of the mothers to CPF caused increase in lipid peroxidation (LPO) and decrease in superoxide dismutase (SOD) and glutathione-s-transferase (GST) in lactating pups. CPF altered the level of the marker parameters related to the liver and kidneys. Consistent histological changes were found in the liver and kidneys of the subjected pups, especially at the higher doses. The results suggested that the transfer of CPF intoxication through the mother’s milk has resulted in oxidative stress and biochemical and histopathological alterations in the suckling pups. The data of this study may be considered as a contribution to the problem of lactational transfer of the relatively less persistent OP pesticides, such as CPF.

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