scholarly journals Antimicrobial and Anti-inflammatory properties of Juglans Regia Leaves

2019 ◽  
Vol 35 (6) ◽  
pp. 1756-1759
Author(s):  
Asma Eswayah ◽  
Nahla Labyad ◽  
Fadilah Aleanizy ◽  
Amal Belaid ◽  
Fulwah Alqahtani ◽  
...  
Keyword(s):  
Extraction Method ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Juglans Regia ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Membrane Stabilization ◽  
Soxhlet Apparatus ◽  
Agar Diffusion Method ◽  
Anti Inflammatory

Antibacterial and anti-inflammatory activities of methanolic extract of Juglans regia L. leaves (Walnut) grown in Libya were investiagted. Hot extraction method by methanol using Soxhlet apparatus was utilized.The antimicrobial effect was studied using agar diffusion method. Human red blood cell membrane stabilization (HRBC) method was used to estimate the anti-inflammatory properties of the leave extract. The extract of leaves exhibited antimicrobial activity against Staphylococcus aureus, and Candida albicans with a minimum inhibitory concentration (MIC) of 25 and 50 mg/ml, respectively. In addition, the extract concentrations of 20, 40, and 100 μg/mL inhibited lysis of HRBC with values of 25.8%, 35.5%, and 70%, respectively. The results showed that the methanolic extract of Juglans regia leaves exhibited antibacterial activity against S. aureus, and antifungal activity against C. albican, and produced anti-inflammatory activity.

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals Determination of antibacterial activity and metabolite profile of Ruta graveolens against Streptococcus mutans and Streptococcus sobrinus

2018 ◽  
Vol 10 (03) ◽  
pp. 320-325 ◽  
Author(s):  
Hamzah Abdulrahman Salman ◽  
Sankarasetty Venkatesh ◽  
Ramasamy Senthilkumar ◽  
B. S. Gnanesh Kumar ◽  
Aamer Mousa Ali
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Plant Material ◽  
Methanolic Extract ◽  
Metabolite Profile ◽  
Diffusion Method ◽  
Ruta Graveolens ◽  
Streptococcus Sobrinus ◽  
Soxhlet Apparatus ◽  
Pure Compounds

ABSTRACT BACKGROUND: Ruta graveolens is one of the most used phytomedicines. To date, there is no report of determining the bioactivity of R. graveolens against cariogenic causing bacteria (Streptococcus mutans and Streptococcus sobrinus). OBJECTIVE: The objective of the present study was to determine the antibacterial activity and metabolite profile of R. graveolens against S. mutans and S. sobrinus. MATERIAL AND METHODS: R. graveolens plant material was collected and processed in the month of February. The plant material was extracted by Soxhlet apparatus using methanol solvent. Two strains of S. mutans and two strains of S. sobrinus were isolated from dental caries-active participants and cultured on mitis salivarius-bacitracin agar. The antibacterial susceptibility testing of methanolic extract of R. graveolens was performed by disc diffusion method. The metabolite profile of the plant extract was determined using electrospray ionization-tandem mass spectrometry. RESULTS: The methanolic extract of R. graveolens showed a promising antibacterial activity against S. mutans and S. sobrinus. Two compounds named γ-fagarine and kokusaginine were identified from the methanolic extract of R. graveolens. CONCLUSIONS: The study concluded that R. graveolens contains significant antibacterial activity. However, further investigations are suggested to understand the anticaries properties of these pure compounds.

scholarly journals A scientific validation of In vitro anti-inflammatory activity of Punica granatum L. by human red blood cell membrane stabilization

2018 ◽  
Vol 6 (7) ◽  
pp. 2430
Author(s):  
Karunakar Kota ◽  
Sandhya Sharma ◽  
Jameela Tahashildar
Keyword(s):  
Methanolic Extract ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Fruit Peel ◽  
Standard Drug ◽  
Membrane Lysis ◽  
Anti Inflammatory ◽  
Punica Granatum L

Background: In recent years there has been growing interest in therapeutic use of natural products, especially those derived from plants. P. granatum is very common dietary ingredient in many parts of India and has remarkable biological and medicinal properties.Methods: In the present study, the methanolic extract of fruit peels of Punica granatum Linn. (MEPG) were investigated for anti-inflammatory activity by simple, reliable, less toxic and less time consuming HRBC membrane stabilization method. The presentation of hypo tonicity induced HRBC membrane lysis was taken a measure of anti-inflammatory activity. Their activities were compared with standard drug diclofenac.Results: The results of the study demonstrated that P. granatum contains active constituents, which possess anti-inflammatory activity which is probably related to the inhibition of prostaglandin synthesis.Conclusions: It is concluded that methanolic extract of P. granatum fruit peel possesses significant anti-inflammatory activity and this is a possible rationale for its folkloric use as an anti-inflammatory agent.

scholarly journals Antioxidant, anti-inflammatory and antimicrobial activities of aqueous and methanolic extract of Rosmarinus eriocalyx Jord. & Fourr.

2020 ◽  
Vol 14 (1) ◽  
pp. 254-262
Author(s):  
Nouioua Wafa ◽  
Gaamoune Sofiane
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory

Rosmarinus eriocalyx Jord. & Fourr is a well-known aromatic and medicinal plant whose consumption serves to remedy a number of disorders, evergreen bush endemic to Algeria. The present study aimed it investigating the in-vitro anti-inflammatory and antimicrobial for two extracts of Rosmarinus eriocalyx. The antioxidant activity was evaluated by DPPH and the reducing power essay, anti-inflammatory activity with the Human Red Blood Cell (HRBC) membrane stabilization method. However, the antimicrobial activity was tested with three bacterial strain Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC25923 and Bacillus subtilis ATCC6633. The results show an excellent antioxidant and an interesting inflammatory activities but a weak power against the used strains therefore. © 2020 International Formulae Group. All rights reserved. Keywords: Rosmarinus eriocalyx Jord. & Fourr, DPPH, reducing power, HRBC, antimicrobial

scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

scholarly journals In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Puneet Singh ◽  
Yogesh Sharma ◽  
Ashutosh Sharma
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Local Market ◽  
Plant Materials ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Convolvulus Pluricaulis

Aim: In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy. Material & Methods- The whole plant parts of Convolvulus pluricaulis Choisy were purchased from the local market. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % methanol by soxhlet apparatus for 72 h. Qualitative chemical tests of methanolic extracts were subjected to various chemical tests to detect various phytoconstituents. Solvent systems ethyl acetate: methanol: water (77:13:10) were found to be most satisfactory solvent system. After development of plates, they were air-dried and number of spots, color and Rf values were recorded. The % heamolysis was calculated by assuming the heamolysis produced by the control group as 100 %. Results: The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 µg/ml, the extract produced 71.59% protection of RBC haemolysis as compared with 72.73% produced by prednisolone.  The methanolic extract of selected plant showed 39.70% inhibition. The Diclofenac sodium showed 55.88 % inhibition against denaturation of protein. Conclusion: In conclusion, it can be stated that the methanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model. Keywords: In-Vitro, Anti-inflammatory Activity, Methanolic Extract, Convolvulus pluricaulis Choisy, Protein Denaturation Method

Phytochemical and pharmacological evaluation of Tragia cannabina for anti-inflammatory activity

2012 ◽  
Vol 1 (8) ◽  
pp. 213 ◽  
Author(s):  
Raju Venkategowda Hosahally ◽  
Ganapaty Seru ◽  
Prasanna Shankarrao Sutar ◽  
Vijaya Gopalachar Joshi ◽  
Kishori Prasanna Sutar ◽  
...  
Keyword(s):  
Body Weight ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Inflammatory Activity ◽  
Reference Standard ◽  
Paw Edema ◽  
Pharmacological Evaluation ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory

<p>The present study investigates the anti-inflammatory activity of methanolic and chloroform extracts of<strong><em> </em></strong><em>Tragia cannabina</em>. The whole plant of <em>Tragia cannabina</em> was extracted with methanol and chloroform by using soxhlet apparatus. The effect of both extracts of <em>Tragia cannabina</em> was studied on carrageenan induced paw edema. The methanolic extract decreased the edema induced in hind paw. The percentage inhibition of paw edema was maximum with methanolic and chloroform extracts of <em>Tragia cannabina</em> at 300mg/kg body weight and has showed significant anti-inflammatory activity. It has been concluded that both the methanolic and chloroform extracts of <em>Tragia cannabina</em> showed significant anti-inflammatory activity comparable to that of reference standard Ibuprofen.</p><p>DOI: <a href="http://dx.doi.org/10.3329/icpj.v1i8.10857">http://dx.doi.org/10.3329/icpj.v1i8.10857</a></p> <p>International Current Pharmaceutical Journal 2012, 1(8): 213-216</p><p> </p>

scholarly journals PHYTOCHEMICAL ANALYSIS AND SUPPRESSION OF INFLAMMATORY TARGETS BY ADATHODA VASICA

2018 ◽  
Vol 11 (5) ◽  
pp. 162
Author(s):  
Amala Reddy ◽  
Sujatha Sundaresan
Keyword(s):  
Methanolic Extract ◽  
Maximum Yield ◽  
Foot Ulcer ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Anti Inflammatory

 Objectives: A persistent and chronic inflammation may act as an underlying definition for many of the long-term illness such as diabetic foot ulcer, Alzheimer’s disease and rheumatoid arthritis. Folklore medicinal plants provide immense health benefits with new remedies. Active compounds from plants containing alkaloids and phenolic compounds (flavonoids and tannins) possess antioxidant and anti-inflammatory activity. The leaves of Adhatoda vasica are most known for its effectiveness in treating respiratory diseases such as asthma, chronic bronchitis, and other respiratory conditions. In this report, we investigated the possible action of A. vasica against inflammation.Methods: Solvents with varying polarity (Hexane, dichloromethane, ethyl acetate, and methanol) were used for the sequential extraction of leaves of A. vasica Linn (Family: Acanthaceae). The anti‐inflammatory activity was assessed using simple in vitro methods. Phenols, flavonoids, phytosterols, saponins, alkaloids, and tannins were analyzed qualitatively using standard protocols. Anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity. Aspirin (diclofenac) was used as a standard drug for the study of anti-inflammatory activity.Results: Our results substantiated that methanolic extract of A. vasica gave a maximum yield of 12.5% and apparently all bioactive phytochemical constituents were at a higher concentration in the methanolic extract. Methanolic extract of A. vasica showed a significant inhibition of the heat-induced albumin denaturation, red blood cells membrane stabilization, and proteinase inhibitory effects with 86±1.46, 88±1.24, and 96±0.93% for 1000 μg/ml, respectively.Conclusion: The above results delineate that the bioactive components were extracted using high polar solvent are mostly flavonoids and related polyphenols. These compounds may be the active constituents to render the anti-inflammatory activity to A. vasica.

Exploring the potential effect of Methanolic extract of Salvia officinalis against UV exposed skin aging: In vivo and In vitro model

2019 ◽  
Vol 12 ◽  
Author(s):  
Ruchi Khare ◽  
Neeraj Upmanyu ◽  
Megha Jha
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Treated Group ◽  
Salvia Officinalis ◽  
Antioxidant Potential ◽  
Meoh Extract ◽  
Soxhlet Apparatus

Context: The medicinal plants have enormous pharmacological properties and having fewer side effects. Today there is increasing demand of medicinal plants as an anti-aging and anti-wrinkle agent. Objective: The aim of this study is to evaluate antioxidant, anti-aging and anti-wrinkle potential of Salvia officinalis. Materials and Methods: Salvia officinalis (Lamiaceae) is folk medicine of Asia and Latin America. Powdered crude drug 100 g were successively extracted in a soxhlet apparatus with petroleum ether (60-80ºC), chloroform and methanol. After successive solvents extraction methanolic extract was used for testing of antioxidant potential using DPPH assay. Further, antiaging potential of extract was investigated by inhibitory effect of various enzymatic estimations i.e. Col-I, Ela-I and Hya-I inhibitory assays on early aging human skin fibroblasts. Antiwrinkle potential of plant Salvia officinalis was done by using UV light induced photo aging model. Results: Phytochemical analysis showed the presence of glycosides, alkaloids flavonoids, and triterpenoids, saponins and Phenolic compounds in high level. Extract showed inhibitory concentration (IC50:24.65) and ascorbic acid the standard antioxidant showed inhibitory concentration (IC50:20.10). In enzymatic estimations assay, the Col-I, Ela-I and Hya-I of extract were assessed showing inhibitory concentration as Col-I (IC50:21.36), Ela-I (IC50:35.05) and Hya-I (IC50:23.44) respectively. Thus, MeOH extract of Salvia officinalis able to inhibit 50% of the activity of aging related enzymes Col-I, Ela-I and Hya-I. The wrinkle score of negative control i.e. UV treated group was 2.83±0.408 and MeOH extract of Salvia officinalis treated group is 1.83 ±0.753. Conclusion: This study concluded that MeOH extract of Salvia officinalis has confirmed the high antioxidant potential and In vitro and In vivo inhibitory potential of antiaging enzymes assessed, thus they could be used for further development of cosmetic products and nutraceuticals.

scholarly journals Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

2014 ◽  
Vol 25 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Flaviana Bombarda de Andrade ◽  
Jair Caetano de Oliveira ◽  
Marjorie Takei Yoshie ◽  
Bruno Martini Guimarães ◽  
Rafael Braga Gonçalves ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Inhibitory Effects ◽  
Broth Macrodilution ◽  
Agar Surface ◽  
In Vitro Effects ◽  
Carious Process ◽  
Microbial Inocula

The present study evaluated the antimicrobial in vitro effects of the salivary proteins lactoferrin and lysozyme on microorganisms involved in the carious process, obtaining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Streptococcus mutans (ATCC 25175) and Lactobacillus casei (ATCC 7469) were submitted to broth macrodilution of lysozyme at 80 mg/mL and lactoferrin at 200 mg/mL. The tubes were read in a spectrophotometer after they had been incubated at 37 °C for 18 h, in a carbon dioxide chamber, in order to read the MIC. A new subculture was carried on agar plates to obtain the MBC. The agar diffusion method was also tested, using BHI agar with 100 µL of the standardized microbial inocula. Filter-paper disks soaked in 10 µL of the solutions lactoferrin (200 µg/mL) and lysozyme (80 µg/mL) were placed on the agar surface. Inhibition halos were not observed on the plates, showing the absence of the antimicrobial effects of these proteins in this method. The bactericidal and bacteriostatic effects of lysozyme on L. casei were 50.3 mg/mL and 43.1 mg/mL respectively. The bactericidal and bacteriostatic effects on S. mutans were 68.5 mg/mL and 58.7 mg/mL. Lactoferrin did not induce any inhibitory effects on any microorganism, even in the concentration of 200 mg/mL. There was not a synergic antimicrobial effect of proteins, when they were tested together, even in the concentration of 42.8 mg/mL of lysozyme and 114 mg/mL of lactoferrin (the highest values evaluated). S. mutans and L. casei were only inhibited by lysozyme, not affected by lactoferrin and by the synergic use of both proteins.

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