Inhibitory Effects of Cinnamon, Clove and Lemongrass Essential Oils against Biofilm Formation by Food Poisoning Bacteria

2021 ◽  
Vol 36 (5) ◽  
pp. 430-439
Author(s):  
Hyeong-Eun Kim ◽  
◽  
Yong-Suk Kim
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Food Poisoning ◽  
Inhibitory Effects

scholarly journals Inhibition of Biofilm Formation in Bacteria by Essential Oils: تثبيط تكون البيوفيلم في البكتيريا بواسطة الزيوت العطرية

2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Mashaeal Saud Alshilawi, Howard Foster
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Research Question ◽  
In Vitro Test ◽  
Test Results ◽  
Inhibitory Effects ◽  
Anova Analysis ◽  
Black Seed ◽  
Vitro Test

The formation of S. epidermidis and P. aeruginosa biofilms were successfully inhibited in the presence of sub-inhibitory concentrations of S. aromaticum (clove) and L. angustifolia (lavender) essential oils. These substances achieved good in-vitro test results. On the other hand, pure and organic types of N. sativa (black seed) essential oil did not exhibit any inhibitory effects on the biofilm formed by the tested bacteria. Although the tested essential oils may share a similar mechanism of action, ANOVA analysis showed strong statistical differences between all essential oils at their sub-MIC levels, and also identified two different trends, biofilm inhibitors, represented by S. aromaticum (clove) and L. angustifolia (lavender) oils, and biofilm stimulators, represented by both types of N. sativa (black seed) oils. A more thorough perspective on the research question could be achieved by considering the various elements that have essentially contributed to the processes and the findings within this study.

Inhibitory effects of the essential oils α-longipinene and linalool on biofilm formation and hyphal growth of Candida albicans

Biofouling ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 143-155 ◽  
Author(s):  
Ranjith Kumar Manoharan ◽  
Jin-Hyung Lee ◽  
Yong-Guy Kim ◽  
Soon-Il Kim ◽  
Jintae Lee
Keyword(s):  
Candida Albicans ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Hyphal Growth ◽  
Inhibitory Effects

Essential oils from Artemisia species inhibit biofilm formation and the virulence of Escherichia coli EPEC 2348/69

Biofouling ◽  
2021 ◽  
pp. 1-11
Author(s):  
Ahmed Mathlouthi ◽  
Nabil Saadaoui ◽  
Eugenia Pennacchietti ◽  
Daniela De Biase ◽  
Mossadok Ben-Attia
Keyword(s):  
Escherichia Coli ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Artemisia Species

scholarly journals Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves and Cloves

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1406
Author(s):  
Rita Cava-Roda ◽  
Amaury Taboada-Rodríguez ◽  
Antonio López-Gómez ◽  
Ginés Benito Martínez-Hernández ◽  
Fulgencio Marín-Iniesta
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Food Packaging ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effects ◽  
Cinnamon Bark ◽  
E Coli ◽  
Pure Compounds ◽  
Main Component

Plant bioactive compounds have antimicrobial and antioxidant activities that allow them to be used as a substitute for synthetic chemical additives in both food and food packaging. To improve its sensory and bactericidal effects, its use in the form of effective combinations has emerged as an interesting possibility in the food industry. In this study, the antimicrobial activities of essential oils (EOs) of cinnamon bark, cinnamon leaves, and clove and the pure compounds vanillin, eugenol, and cinnamaldehyde were investigated individually and in combination against Listeria monocytogenes and Escherichia coli O157:H7. The possible interactions of combinations of pure compounds and EOs were performed by the two-dimensional checkerboard assay and isobologram methods. Vanillin exhibited the lowest antimicrobial activity (MIC of 3002 ppm against L. monocytogenes and 2795 ppm against E. coli O157:H7), while clove and cinnamon bark EOs exhibited the highest antimicrobial activity (402–404 against L. monocytogenes and 778–721 against E. coli O157:H7). For L. monocytogenes, pure compound eugenol, the main component of cinnamon leaves and clove, showed lower antimicrobial activity than EOs, which was attributed to the influence of the minor components of the EOs. The same was observed with cinnamaldehyde, the main component of cinnamon bark EO. The combinations of vanillin/clove EO and vanillin/cinnamon bark EO showed the most synergistic antimicrobial effect. The combination of the EOs of cinnamon bark/clove and cinnamon bark/cinnamon leaves showed additive effect against L. monocytogenes but indifferent effect against E. coli O157:H7. For L. monocytogenes, the best inhibitory effects were achieved by cinnamon bark EO (85 ppm)/vanillin (910 ppm) and clove EO (121 ppm)/vanillin (691 ppm) combinations. For E. coli, the inhibitory effects of clove EO (104 ppm)/vanillin (1006 ppm) and cinnamon leaves EO (118 ppm)/vanillin (979 ppm) combinations were noteworthy. Some of the tested combinations increased the antimicrobial effect and would allow the effective doses to be reduced, thereby offering possible new applications for food and active food packaging.

Identification of an antifungal peptide from Trapa natans fruits with inhibitory effects on Candida tropicalis biofilm formation

Peptides ◽  
2011 ◽  
Vol 32 (8) ◽  
pp. 1741-1747 ◽  
Author(s):  
Santi M. Mandal ◽  
Ludovico Migliolo ◽  
Octavio L. Franco ◽  
Ananta K. Ghosh
Keyword(s):  
Candida Tropicalis ◽  
Biofilm Formation ◽  
Antifungal Peptide ◽  
Inhibitory Effects ◽  
Trapa Natans

scholarly journals Effect of Essential Oils on Growth Inhibition, Biofilm Formation and Membrane Integrity of Escherichia coli and Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1474
Author(s):  
Andrés Martínez ◽  
Marcela Manrique-Moreno ◽  
Maria C. Klaiss-Luna ◽  
Elena Stashenko ◽  
German Zafra ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Biofilm Formation ◽  
Method Development ◽  
Membrane Integrity ◽  
Thymus Vulgaris ◽  
Hydrophobic Core ◽  
Synthetic Membranes ◽  
E Coli ◽  
Microdilution Method

Biofilm as a cellular conformation confers survival properties to microbial populations and favors microbial resistance. Here, we investigated the antimicrobial, antibiofilm, antimotility, antihemolytic activity, and the interaction with synthetic membranes of 15 essential oils (EOs) on E. coli ATCC 25922 and S. aureus ATCC 29213. Antimicrobial activity of EOs was determined through microdilution method; development of the biofilm was assessed using the crystal violet assay and SEM microscopy. Results indicate that Lippia origanoides thymol–carvacrol II chemotype (LTC II) and Thymus vulgaris (TV) exhibited a significant antibacterial activity, with MIC values of 0.45 and 0.75 mg/mL, respectively. The percentage of biofilm formation inhibition was greater than 70% at subinhibitory concentrations (MIC50) for LTC II EO. The results demonstrate that these two oils had significantly reduced the hemolytic effect of S. aureus by 54% and 32%, respectively, and the mobility capacity by swimming in E. coli with percentages of decrease of 55% and 47%, respectively. The results show that LTC II and TV EOs can interact with the hydrophobic core of lipid bilayers and alter the physicochemical properties of membranes. The findings suggest that LTC II and TV oils may potentially be used to aid in the treatment of S. aureus and E. coli infections.

scholarly journals Antimicrobial and antibiofilm properties of essential oils from Piper marginatum Jacq.

2021 ◽  
Vol 10 (11) ◽  
pp. e514101119967
Author(s):  
Ana Lúcia Mendes dos Santos ◽  
Filipe Augusto Matos Araújo ◽  
Érika da Silva Matisui ◽  
Luiz Antonio Mendonça Alves da Costa ◽  
Alexandre José Macêdo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Folk Medicine ◽  
Gram Negative Bacteria ◽  
Biofilm Inhibition ◽  
Future Developments ◽  
Phytochemical Constituents ◽  
Amazonian Region

A low shrub growing in the Amazonian region, Piper marginatum Jacq. has been related to the treatment of a disease variety in folk medicine, however, still lacking scientific support. This study aimed to describe the composition of essential oils obtained from leaves (EOL) and branches (EOB) of P. marginatum and their antimicrobial effects on six relevant pathogenic bacteria. A combination of GC-FID and GC-MS was used to identify the phytochemical constituents. As antimicrobial assays, the oils were screened at the minimum inhibitory concentration (MIC) of 3 µg/ml for planktonic and biofilm inhibition. EOL revealed the presence of trans–nerolidol, o–cymene, spathulenol, elemicin, and α–copaene, while EOB composition was mainly of myristicin, trans-caryophyllene, trans-nerolidol, caryophyllene oxide, α–copaene, γ–muurolene and spathulenol. The strongest inhibition of planktonic growth was achieved against Pseudomonas aeruginosa (EOB) and Escherichia coli (EOB). Overall, Gram negative bacteria were more sensitive to both EOB/EOL showing less ability of growth and biofilm formation. The Gram-positive strains seemed to react to the essential oils by massive adhesion. Our results corroborate the relevance of Piperaceae and indicate the possible use of P. marginatum in future developments of antimicrobials.

scholarly journals Novel Method for The Valorization of Essential Oils From Orange By-Product

2021 ◽  
Author(s):  
Roudaina Abdel Samad ◽  
Karim Raafat ◽  
Alissar Al Khatib ◽  
Hadi Abou Chacra ◽  
nada EL DARRA
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Bacterial Load ◽  
Minimum Energy ◽  
Bacterial Biofilm ◽  
Diffusion Method ◽  
Oil Composition ◽  
By Products ◽  
Orange Essential Oil

Abstract Background: A huge amount of citrus by-products are being wasted every year; these by-products can be used in the extraction of essential oils which can be used in different fields. Many methods have been used in the extraction of orange essential oil (E.O). This study focuses on the extraction of essential oil from orange by-product, using a newly developed extraction technique. This technique is a prototype combining a conventional method, namely, the hydrodistillation with the ultrasound-assisted extraction. Indeed, the traditional extraction methods of essential oils are time-consuming and expensive, moreover many organic compounds may decompose at high retained temperatures.Methods: a combination of traditional method with sonication improves efficiency and quality of the extraction, since ultrasonic extraction with precise and controlled operating parameters leads to higher extraction yields with minimum energy consumption. Therefore, the orange essential oil obtained using the prototype was compared to two commercial oils (CO1 and CO2) to create a valid comparison. Quality and safety tests have been performed, as well as bacterial load, antibacterial activities using the disc diffusion method, minimum inhibitory concentration (MIC), and prevention of bacterial biofilm formation.Results: As a result of extraction, the prototype method has shorten the extraction time, gave a higher quantity, and improved the extraction of essential oil from citrus peels without affecting oil composition when comparing it to hydrodistillation method. Conclusion: Orange E.O can be used as natural antibacterial agents to minimize bacterial growth, and inhibit biofilm formation by Staphylococcus aureus, Listeria monocytogenes and E.coli.

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