scholarly journals Gibberellin induced transcription factor bZIP53 regulates CesA1 expression in maize kernels

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0244591
Author(s):  
Huayang Lv ◽  
Xiao Li ◽  
Hui Li ◽  
Yufeng Hu ◽  
Hanmei Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Cellulose Synthase ◽  
Cellulose Synthesis ◽  
Cellulose Content ◽  
Hormone Regulation ◽  
Grain Development ◽  
Cellulose Synthase Gene ◽  
Maize Grain ◽  
Maize Kernels

Proper development of the maize kernel is of great significance for high and stable maize yield to ensure national food security. Gibberellin (GA), one of the hormones regulating plant growth, is involved in modulating the development of maize kernels. Cellulose, one of the main components of plant cells, is also regulated by gibberellin. The mechanism of hormone regulation during maize grain development is highly complicated, and reports on GA-mediated modulation of cellulose synthesis during maize grain development are rare. Our study revealed that during grain growth and development, the grain length and bulk density of GA-treated corn kernels improved significantly, and the cellulose content of grains increased, while seed coat thickness decreased. The transcription factor basic region/leucine zipper motif 53 (bZIP53), which is strongly correlated with cellulose synthase gene 1 (CesA1) expression, was screened by transcriptome sequencing and the expression of the cellulose synthase gene in maize grain development after GA treatment was determined. It was found that bZIP53 expression significantly promoted the expression of CesA1. Further, analysis of the transcription factor bZIP53 determined that the gene-encoded protein was localized in the cell and nuclear membranes, but the transcription factor bZIP53 itself showed no transcriptional activation. Further studies are required to explore the interaction of bZIP53 with CesA1.

scholarly journals Disruption of Very-Long-Chain-Fatty Acid Synthesis Has an Impact on the Dynamics of Cellulose Synthase in Arabidopsis thaliana

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1599
Author(s):  
Xiaoyu Zhu ◽  
Frédérique Tellier ◽  
Ying Gu ◽  
Shundai Li
Keyword(s):  
Fatty Acid ◽  
Cell Biology ◽  
Higher Plants ◽  
Threshold Level ◽  
Cellulose Synthase ◽  
Chain Fatty Acid ◽  
Cellulose Synthesis ◽  
Cellulose Content ◽  
Long Chain Fatty Acid ◽  
Long Chain

In higher plants, cellulose is synthesized by membrane-spanning large protein complexes named cellulose synthase complexes (CSCs). In this study, the Arabidopsis PASTICCINO2 (PAS2) was identified as an interacting partner of cellulose synthases. PAS2 was previously characterized as the plant 3-hydroxy-acyl-CoA dehydratase, an ER membrane-localized dehydratase that is essential for very-long-chain-fatty acid (VLCFA) elongation. The pas2-1 mutants show defective cell elongation and reduction in cellulose content in both etiolated hypocotyls and light-grown roots. Although disruption of VLCFA synthesis by a genetic alteration had a reduction in VLCFA in both etiolated hypocotyls and light-grown roots, it had a differential effect on cellulose content in the two systems, suggesting the threshold level of VLCFA for efficient cellulose synthesis may be different in the two biological systems. pas2-1 had a reduction in both CSC delivery rate and CSC velocity at the PM in etiolated hypocotyls. Interestingly, Golgi but not post-Golgi endomembrane structures exhibited a severe defect in motility. Experiments using pharmacological perturbation of VLCFA content in etiolated hypocotyls strongly indicate a novel function of PAS2 in the regulation of CSC and Golgi motility. Through a combination of genetic, biochemical and cell biology studies, our study demonstrated that PAS2 as a multifunction protein has an important role in the regulation of cellulose biosynthesis in Arabidopsis hypocotyl.

scholarly journals TRANVIA (TVA) facilitates cellulose synthase trafficking and delivery to the plasma membrane

2021 ◽  
Vol 118 (30) ◽  
pp. e2021790118
Author(s):  
Tamara Vellosillo ◽  
José R. Dinneny ◽  
Chris R. Somerville ◽  
David W. Ehrhardt
Keyword(s):  
Plasma Membrane ◽  
Molecular Mechanisms ◽  
Cellulose Synthase ◽  
Specific Protein ◽  
Primary Cell Wall ◽  
Cellulose Synthesis ◽  
Cellulose Content ◽  
Enhanced Sensitivity ◽  
Intracellular Compartments ◽  
Secretory System

Cellulose is synthesized at the plasma membrane by cellulose synthase (CESA) complexes (CSCs), which are assembled in the Golgi and secreted to the plasma membrane through the trans-Golgi network (TGN) compartment. However, the molecular mechanisms that guide CSCs through the secretory system and deliver them to the plasma membrane are poorly understood. Here, we identified an uncharacterized gene, TRANVIA (TVA), that is transcriptionally coregulated with the CESA genes required for primary cell wall synthesis. The tva mutant exhibits enhanced sensitivity to cellulose synthesis inhibitors; reduced cellulose content; and defective dynamics, density, and secretion of CSCs to the plasma membrane as compared to wild type. TVA is a plant-specific protein of unknown function that is detected in at least two different intracellular compartments: organelles labeled by markers for the TGN and smaller compartments that deliver CSCs to the plasma membrane. Together, our data suggest that TVA promotes trafficking of CSCs to the plasma membrane by facilitating exit from the TGN and/or interaction of CSC secretory vesicles with the plasma membrane.

scholarly journals Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shuangtao Li ◽  
Lei Zhang ◽  
Ying Wang ◽  
Fengfeng Xu ◽  
Mengyun Liu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Leaf Anatomy ◽  
Cellulose Synthase ◽  
Cellulose Content ◽  
Cellulose Synthase Gene

The expression of a rice secondary wall-specific cellulose synthase gene, OsCesA7, is directly regulated by a rice transcription factor, OsMYB58/63

Planta ◽  
2015 ◽  
Vol 242 (3) ◽  
pp. 589-600 ◽  
Author(s):  
Soichiro Noda ◽  
Taichi Koshiba ◽  
Takefumi Hattori ◽  
Masatoshi Yamaguchi ◽  
Shiro Suzuki ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Secondary Wall ◽  
Cellulose Synthase ◽  
Cellulose Synthase Gene

scholarly journals Cross-species PCR and field studies on Paulownia elongata: A potential bioenergy crop

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Chhandak Basu ◽  
Nirmal Joshee ◽  
Tigran Gezalian ◽  
Brajesh Nanda Vaidya ◽  
Asada Satidkit ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Plant Cell Wall ◽  
Cellulose Synthase ◽  
Cellulose Synthesis ◽  
Acid Extraction ◽  
Dot Blot ◽  
Carbon Sequestration Potential ◽  
Sequestration Potential ◽  
Cellulose Synthase Gene ◽  
Paulownia Elongata

AbstractPaulownia elongata is a short-rotation fast growing tree and is known for high biomass accumulation and carbon sequestration potential. Optimization of protocols for nucleic acid extraction, PCR, RT-PCR, and other molecular biology techniques are required for better understanding of cellulose synthesis and to assess the potential of Paulownia as a biofuel tree. The main objective of this work was to study a putative cellulose synthase amplicon expression under various environmental conditions and evaluate the potentials of Paulownia as a biofuel tree. Using cross-species PCR an amplicon representative of a putative cellulose synthase gene from Paulownia was identified. This 177-bp long DNA sequence was 46% similar with cellulose synthase genes from Arabidopsis as expected. Gene specific primers for this particular Paulownia cellulose synthase gene were designed and reverse transcription PCR was performed to confirm its transcription. We report an inexpensive cDNA dot-blot method to study expression of this gene under various environmental conditions. We observed that cold and, to a lesser extent, heat stress downregulated its expression. This information will help to understand cellulose deposition in plant cell wall under stressful conditions. To the best of our knowledge this is the first characterization of a cDNA sequence from Paulownia elongata.

Analysis of the moisture content of maize kernels in over-ripe plants

2006 ◽  
Vol 54 (4) ◽  
pp. 425-430
Author(s):  
T. Árendás ◽  
L. C. Marton ◽  
P. Bónis ◽  
Z. Berzsenyi
Keyword(s):  
Linear Regression ◽  
Moisture Content ◽  
Grain Yield ◽  
Equilibrium Moisture Content ◽  
Weather Conditions ◽  
Positive Correlation ◽  
Maize Grain Yield ◽  
Maize Grain ◽  
Maize Kernels

The effect of varying weather conditions on the moisture content of the maize grain yield was investigated in Martonvásár, Hungary from late August to late September, and from the 3rd third of September to the 1st third of Novemberbetween 1999 and 2002. In every year a close positive correlation (P=0.1%) could be observed between the moisture content in late September and the rate of drying down in October. Linear regression was used each year to determine the equilibrium moisture content, to which the moisture content of kernels returned if they contained less than this quantity of water in late September and harvesting was delayed. In the experimental years this value ranged from 15.24-19.01%.

Subnuclear compartmentalization of sequence-specific transcription factors and regulation of eukaryotic gene expression

2005 ◽  
Vol 83 (4) ◽  
pp. 535-547 ◽  
Author(s):  
Gareth N Corry ◽  
D Alan Underhill
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Transcriptional Activation ◽  
Current Knowledge ◽  
Nuclear Architecture ◽  
Subnuclear Localization ◽  
Modular Structures

To date, the majority of the research regarding eukaryotic transcription factors has focused on characterizing their function primarily through in vitro methods. These studies have revealed that transcription factors are essentially modular structures, containing separate regions that participate in such activities as DNA binding, protein–protein interaction, and transcriptional activation or repression. To fully comprehend the behavior of a given transcription factor, however, these domains must be analyzed in the context of the entire protein, and in certain cases the context of a multiprotein complex. Furthermore, it must be appreciated that transcription factors function in the nucleus, where they must contend with a variety of factors, including the nuclear architecture, chromatin domains, chromosome territories, and cell-cycle-associated processes. Recent examinations of transcription factors in the nucleus have clarified the behavior of these proteins in vivo and have increased our understanding of how gene expression is regulated in eukaryotes. Here, we review the current knowledge regarding sequence-specific transcription factor compartmentalization within the nucleus and discuss its impact on the regulation of such processes as activation or repression of gene expression and interaction with coregulatory factors.Key words: transcription, subnuclear localization, chromatin, gene expression, nuclear architecture.

scholarly journals Transcriptional Activation in Yeast Cells Lacking Transcription Factor IIA

Genetics ◽  
1999 ◽  
Vol 153 (4) ◽  
pp. 1573-1581 ◽  
Author(s):  
Susanna Chou ◽  
Sukalyan Chatterjee ◽  
Mark Lee ◽  
Kevin Struhl
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Large Subunit ◽  
Yeast Cells ◽  
Specific Cell ◽  
Wild Type ◽  
Activation Domains ◽  
Cycle Arrest ◽  
General Transcription Factor

Abstract The general transcription factor IIA (TFIIA) forms a complex with TFIID at the TATA promoter element, and it inhibits the function of several negative regulators of the TATA-binding protein (TBP) subunit of TFIID. Biochemical experiments suggest that TFIIA is important in the response to transcriptional activators because activation domains can interact with TFIIA, increase recruitment of TFIID and TFIIA to the promoter, and promote isomerization of the TFIID-TFIIA-TATA complex. Here, we describe a double-shut-off approach to deplete yeast cells of Toa1, the large subunit of TFIIA, to <1% of the wild-type level. Interestingly, such TFIIA-depleted cells are essentially unaffected for activation by heat shock factor, Ace1, and Gal4-VP16. However, depletion of TFIIA causes a general two- to threefold decrease of transcription from most yeast promoters and a specific cell-cycle arrest at the G2-M boundary. These results indicate that transcriptional activation in vivo can occur in the absence of TFIIA.

scholarly journals NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

2021 ◽  
Vol 22 (13) ◽  
pp. 6952
Author(s):  
Mingxin Yu ◽  
Junling Liu ◽  
Bingshuai Du ◽  
Mengjuan Zhang ◽  
Aibin Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stress Response ◽  
Transcriptional Activation ◽  
Dominant Role ◽  
State Level ◽  
Energy Conversion Efficiency ◽  
Nac Transcription Factor ◽  
Wild Plants ◽  
Transgenic Lines

NAC (NAM, ATAF1/2, and CUC2) transcription factors are ubiquitously distributed in eukaryotes and play significant roles in stress response. However, the functional verifications of NACs in Picea (P.) wilsonii remain largely uncharacterized. Here, we identified the NAC transcription factor PwNAC11 as a mediator of drought stress, which was significantly upregulated in P. wilsonii under drought and abscisic acid (ABA) treatments. Yeast two-hybrid assays showed that both the full length and C-terminal of PwNAC11 had transcriptional activation activity and PwNAC11 protein cannot form a homodimer by itself. Subcellular observation demonstrated that PwNAC11 protein was located in nucleus. The overexpression of PwNAC11 in Arabidopsis obviously improved the tolerance to drought stress but delayed flowering time under nonstress conditions. The steady-state level of antioxidant enzymes’ activities and light energy conversion efficiency were significantly increased in PwNAC11 transgenic lines under dehydration compared to wild plants. PwNAC11 transgenic lines showed hypersensitivity to ABA and PwNAC11 activated the expression of the downstream gene ERD1 by binding to ABA-responsive elements (ABREs) instead of drought-responsive elements (DREs). Genetic evidence demonstrated that PwNAC11 physically interacted with an ABA-induced protein—ABRE Binding Factor3 (ABF3)—and promoted the activation of ERD1 promoter, which implied an ABA-dependent signaling cascade controlled by PwNAC11. In addition, qRT-PCR and yeast assays showed that an ABA-independent gene—DREB2A—was also probably involved in PwNAC11-mediated drought stress response. Taken together, our results provide the evidence that PwNAC11 plays a dominant role in plants positively responding to early drought stress and ABF3 and DREB2A synergistically regulate the expression of ERD1.

Sign in / Sign up

Export Citation Format

Share Document