scholarly journals In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

2020 ◽  
Vol 13 (8) ◽  
pp. 1549-1554
Author(s):  
Zein Ahmad Baihaqi ◽  
Irkham Widiyono ◽  
Wisnu Nurcahyo
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Sem Analysis ◽  
The Body ◽  
Paraserianthes Falcataria ◽  
Petri Dishes ◽  
Ethanol Extracts

Aim: This study was conducted to determine the anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark against Haemonchus contortus. Materials and Methods: Ethanol extract of bark (E.E.B.) waste and aqueous extract of bark (A.E.B.) waste of P. falcataria (at concentrations 0, 0.2, 0.4, 0.8, 1, 2.5, and 5%) and albendazole (2 mg/ml) as the positive control were placed in separate Petri dishes (50 mm). Twenty H. contortus worms were placed in Petri dishes and incubated at 37°C for 0.5, 1, 2, 3, 4, 5, 6, and 12 h. Mortality of each worm was ensured by pressing the body of the worm with a pair of tweezers and keeping it in lukewarm water for 5 min before declaring it dead. Mortality is defined as amount of death individuals and time of mortality of each worm was recorded. The parasites were then observed using scanning electron microscopy (SEM) at an accelerating voltage of 15 Kv. Statistical analysis was performed using SPSS 21.0 software, two-way ANOVA followed by Tukey's test to detect significant differences (p<0.05). The result was expressed as the mean ± standard deviation. Results: The E.E.B. and A.E.B. of P. falcataria contained active compounds, such as tannin, alkaloid, flavonoid, saponin, steroid, and triterpenoid. E.E.B. had a higher content of phenol, while A.E.B. had a higher content of flavonoid. In this study, P. falcataria showed a significant effect (p=0.00) on H. contortus in vitro. E.E.B. (0.8%) was able to exterminate H. contortus completely after 6 h, more effective than A.E.B. (5%) while the positive control requires (2 mg/ml) after 2 h. SEM analysis of the worm treated with E.E.B. and A.E.B. showed damaged cuticle structure. Conclusion: The aqueous and ethanol extracts of P. falcataria bark waste demonstrated anthelmintic activity against H. contortus.

scholarly journals EFEK EKSTRAK ETANOL BIJI LABU KUNING (CUCURBITA MOSCHATA DUCHESNE) SEBAGAI ANTELMINTIK PADA CACING GELANG (ASCARIDIA GALLI)

2020 ◽  
Vol 7 (1) ◽  
pp. 11-18
Author(s):  
Noni Zakiah ◽  
Vonna Aulianshah ◽  
T. Maulana Hidayatullah ◽  
Faridah Hanum
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
The Body ◽  
Whole Body ◽  
In Vitro Test ◽  
Cucurbita Moschata ◽  
Ascaridia Galli ◽  
Group I ◽  
Pumpkin Seeds

Kegunaan labu kuning di Indonesia masih sebatas daging buah yang dapat diolah menjadi panganan seperti kue basah, kolak dan sayur berkuah. Secara empiris, biji labu kuning telah digunakan untuk mengatasi cacingan. Penelitian ini dilakukan untuk mengetahui mortalitas cacing gelang (Ascaridia galli) dalam ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne). Penelitian ini menggunakan 25 ekor Ascaridia galli yang dibagi menjadi 5 kelompok, kelompok I kontrol negatif menggunakan larutan NaCl fisiologis, kelompok II kontrol positif menggunakan larutan pirantel pamoat 0,5 %, kelompok III, IV dan V berturut-turut menggunakan 25 mg/ml, 50 mg/ml dan 100 mg/ml ekstrak etanol biji labu kuning. Parameter penelitian ini ditentukan dengan melihat persentase nilai skor pasca inkubasi 12 jam, 24 jam, dan 36 jam. Skor 3 diberikan apabila seluruh tubuh Ascaridia galli bergerak, skor 2 diberikan jika hanya sebagian tubuh Ascaridia galli bergerak, skor 1 jika Ascaridia galli diam tetapi masih hidup, dan skor 0 apabila Ascaridia galli mati. Hasil uji in vitro dengan perlakuan 25 mg/ml ekstrak etanol biji labu kuning menyebabkan kematian 3 ekor Ascaridia galli  atau 60% pasca inkubasi 36 jam, sedangkan ekstrak etanol biji labu kuning dengan perlakuan 50 mg/ml, 100 mg/ml dan kelompok kontrol positif mengakibatkan kematian 4 ekor Ascaridia galli atau 80% pasca inkubasi 36 jam. Dari hasil penelitian disimpulkan bahwa ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne) dosis 25 mg/ml, 50 mg/ml, dan 100 mg/ml secara in vitro dalam waktu 36 jam mampu mengakibatkan mortalitas Ascaridia galli. The use of yellow pumpkin in Indonesia is still limited to fruit meat that can be processed into snacks such as soggy cakes, porridge and vegetable soup. This research was conducted to determine the mortality of Ascaridia galli in ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne). This study used 25 Ascaridia galli which were divided into 5 groups, group I was negative control using physiological NaCl solution, group II was positive control using 0.5% pirantel pamoate solution, group III, IV and V respectively used 25 mg / ml, 50 mg/ml and 100 mg/ml ethanol extract of yellow pumpkin seeds. The parameters of this study were determined by looking at the percentage of post-incubation scores 12 hours, 24 hours, and 36 hours. A score of 3 is given if the whole body of Ascaridia galli moves, a score of 2 is given if only part of the body of Ascaridia galli moves, a score of 1 if Ascaridia galli is still but still alive, and a score of 0 if Ascaridia galli dies. In vitro test results with 25 mg/ml ethanol extract of pumpkin seeds caused 3 deaths of Ascaridia galli or 60% after incubation for 36 hours, while ethanol extract of yellow pumpkin seeds treated with 50 mg / ml, 100 mg/ml and positive control group resulting in the death of 4 Ascaridia galli or 80% after 36 hours incubation. From the results of the study concluded that the ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne) doses of 25 mg / ml, 50 mg / ml, and 100 mg / ml in vitro within 36 hours can lead to Ascaridia galli mortality.

In vitro evaluation of antisickling activity of herbal combination of three medicinal plants

2020 ◽  
Vol 23 ◽  
pp. 54-62
Author(s):  
O.O. Amujoyegbe ◽  
M. Idu ◽  
J.M. Agbedahunsi ◽  
G.N. Bazuaye
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Cymbopogon Citratus ◽  
Piper Guineense ◽  
Standard Drug ◽  
African People ◽  
Ethanol Extracts ◽  
Gongronema Latifolium ◽  
Cell Disorder

Sickle cell disorder is a genetic ailment with enormous social and economic burden for patients and caregivers. The most promising management apart from being expensive particularly for poor African people, faces some major incompatibility problems. The patients consequently rely on herbal therapy which could be prepared in single or combination forms to manage the painful episodes and its complications. This present study aimed to formulate polyherbal combination and evaluate three purposively selected plants previously reported for their antisickling activities. The polyherbal products were formulated using both aqueous and 70% ethanol extracts into different combinational ratio with the best in 1:1:1 and evaluated for its antisickling activity. The antisickling activity involved both the inhibitory and reversal effects at varying concentrations from 1.0 mg/ml to 6.0 mg/ml using ciklavit as the positive control. The best inhibitory activity was found in ethanol extract of Piper guineense, Gongronema latifolium and Cymbopogon citratus (PGC) with 70.09 ± 0.67% when compared with the positive control (59.25 ± 0.05%) at 4.0 mg/mg while the reversal ability was 67.87 ± 1.23%. The aqueous extracts of the combinations had activity above 50% with the exception of PGC (2:3:1) which is a little less than 50% (46.67 ± 0.98%) while the highest was 60.02 ± 0.87%. The polyherbal ethanol extract had better effects than the aqueous extract and the standard drug used in this study.

In vitro anthelmintic activity of crude extracts of selected medicinal plants against Haemonchus contortus from sheep

2012 ◽  
Vol 87 (2) ◽  
pp. 174-179 ◽  
Author(s):  
M. Ahmed ◽  
M.D. Laing ◽  
I.V. Nsahlai
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Allium Sativum ◽  
Anthelmintic Activity ◽  
Ananas Comosus ◽  
Lespedeza Cuneata ◽  
Aloe Ferox ◽  
Ethanol Extracts

AbstractEthanol extracts of 25 plant species were screened for anthelmintic effects against Haemonchus contortus. Ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°C with control cultures which were treated with ethanol for 48 h. Five plants with high efficacies (Ananas comosus, Aloe ferox, Allium sativum, Lespedeza cuneata and Warburgia salutaris) were selected from the first screening for further investigation using ethanol, dichloromethane and water extracts at four concentrations (2.5, 5, 10 and 20%). Ethanol was the most effective solvent. Larval counts decreased with increasing extract concentrations, of which 10 and 20% had similar effects. Lespedeza cuneata caused more than 70% mortality at all concentrations. However, there remains a need to assess in vivo efficacy of these plants.

In vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla (Oxalidaceae) hydroalcoholic extract against Haemonchus contortus in lambs

2017 ◽  
Vol 92 (3) ◽  
pp. 309-316 ◽  
Author(s):  
B.J. González-Cruz ◽  
M. Rodríguez-Labastida ◽  
M. González-Cortázar ◽  
A. Zamilpa ◽  
M.E. López-Arellano ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Larval Mortality ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Plate Method ◽  
Hydroalcoholic Extract ◽  
Stage 1 ◽  
Two Stages

AbstractThe in vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla hydroalcoholic extract (HE), against Haemonchus contortus in experimentally infected lambs, were assessed. We used a microtitration plate method, comprising the following two stages. Stage 1: 20 μl of water containing 200 sheathed H. contortus infective larvae (ShHcl) were deposited in every well of three series; then, the series 2 and 3 wells were treated with 80 μl 1% ivermectin and O. tetraphylla HE at 20 mg/ml, respectively. Stage 2: the same procedure was performed replacing the ShHcl with exsheathed larvae (ExShHcl). Evaluations were performed after 24 and 48 h. The total numbers of dead and live larvae were counted. A second experiment evaluated the reduction in nematode egg populations in the faeces of lambs treated orally with the O. tetraphylla HE. The 27 lambs used were divided into Groups 1, 2 and 3 (n = 9), which were administered water (positive control), levamisole 1 m (7.5 mg/kg body weight (BW), as a unique dose) and O. tetraphylla HE (20 mg/kg BW), respectively. The plant HE was administered daily for 8 days. The in vitro assay showed 80.9% and 86.5% larval mortality of ShHcl after 24 and 48 h, respectively, while the corresponding mortality values for ExShHcl were 97 and 99%, respectively. The in vivo assay showed variability in the eggs/gram of faeces (epg) values; however, at the end of the trial, the average reduction in the epg values of the O. tetraphylla HE group was 45.6% (P < 0.05). Oxalis tetraphylla HE contains compounds that belong to the flavonol group with anthelmintic activity.

Evaluation of Anthelmintic properties of Sesbania grandiflora Pers. (Kathurumurunga) against larvae of Toxocara canis and Haemonchus contortus – In Vitro Study

The Healer ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 42-47
Author(s):  
ERHSS Ediriweera
Keyword(s):  
Sri Lanka ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Toxocara Canis ◽  
Positive Control ◽  
Migration Inhibition ◽  
Larval Migration ◽  
Sesbania Grandiflora

Sesbania grandiflora is a slender tree. It is a common garden plant in Sri Lanka, which grows well in mid and low country. In Sri Lanka, Sesbania grandiflora is used as a home remedy, in treatment of worm infections in humans. Toxocara canis is a helminth parasite infecting dogs and other canids. It also causes toxocariasis in humans. Haemonchus contortus is a nematode that infects goats and causes anaemia, marked reduction in growth and reproduction, and even death. Using this information, in vitro larvae migratory inhibition assay was carried out on Toxocara canis and Haemonchus contortus larve. This study revealed 98.1% and 94.3% larvae migration inhibition with larvae of Toxocara canis and Haemonchus contortus respectively. Least number of migrated larvae was observed in the positive control Levamisole and all the larvae were dead after migration. In decoction of S.grandiflora, all the migrated Toxocara larvae were dead and Haemonchus larvae were dead or in Grade1 (inactive but occasional movement can be observed) condition. Inhibition of Toxocara larval migration and Haemonchus larval migration with decoction of Sesbania grandiflora and Levamisole are statically significant (p < 0.05). Since mean of LMI (larval migration inhibition) of Levamisole is greater than mean of LMI of Sesbania grandiflora with both larvae, Levamisole is more effective than Sesbania grandiflora. Based on these findings, the aqueous extract of leaves of Sesbania grandiflora is shows a statistically significant anthelmintic activity in in-vitro model.

scholarly journals Anti-Inflammatory, Antioxidant, and Anthelmintic Activities of Ocimum basilicum (Sweet Basil) Fruits

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Clement Osei Akoto ◽  
Akwasi Acheampong ◽  
Yaw Duah Boakye ◽  
Abdulai A. Naazo ◽  
Derrick H. Adomah
Keyword(s):  
Antioxidant Activities ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
High Concentration ◽  
Hexane Extracts ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Dpph Scavenging

Basil (Ocimum basilicum Linn.) is a plant that has found use in traditional medicine and household remedy against human ailments. The present study aimed at investigating hexane and ethanol fruit extracts of Ocimum basilicum Linn. for their phytoconstituents and anti-inflammatory, antioxidant, and anthelmintic activities. Phytochemical screenings were performed using standard protocols. Anti-inflammatory activities were assessed using the egg albumin denaturation method, while the antioxidant activities of the extracts were determined using DPPH scavenging, total antioxidant capacity (TAC), and hydrogen peroxide (H2O2) assays. In vitro anthelmintic activity of both extracts was investigated against Eudrilus eugeniae (Earthworms). Column chromatography was employed to separate the least polar fraction A, using hexane as the mobile phase, which was analyzed using FTIR analysis. The preliminary phytochemical screening revealed the presence of alkaloids, phenols, saponins, glycosides, steroids, tannins, terpenoids, and flavonoids. The hexane and ethanol extracts exhibited high concentration-dependent anti-inflammatory activities, with their percentage inhibitions being 34.0 ± 0.01 and 17.6 ± 0.04 μg/mL, respectively. The hexane extract showed a higher concentration-dependent antioxidant activity (IC50 of 14.17 ± 1.15 μg/mL) compared to the ethanol extract (IC50 > 100.0 μg/mL) for the DPPH assay. The IC50 values of ethanol and hexane extracts were 623.5 ± 0.27 and >1000.0 μg/mL, respectively, for the H2O2 scavenging assay. TAC was determined to be 37.48 ± 0.09 and 18.81 ± 0.12 gAAE/100 g for ethanol and hexane extracts, respectively. Both extracts showed a higher concentration-dependent anthelmintic activity. Increasing the concentration increased the helmintic potency of the extracts. The results indicate that both hexane and ethanol extracts of Ocimum basilicum Linn. exhibit anti-inflammatory, antioxidant, and anthelmintic activities and support the traditional usage of this plant.

scholarly journals Studies on medicinal plants against gastroinstestinal nematodes of goats

1970 ◽  
Vol 6 (2) ◽  
pp. 179-183 ◽  
Author(s):  
MA Sujon ◽  
M Mostofa ◽  
MS Jahan ◽  
AR Das ◽  
S Rob
Keyword(s):  
Medicinal Plants ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Gastrointestinal Nematodes ◽  
Variable Degree ◽  
Indigenous Plants ◽  
Significant Efficacy ◽  
Ethanol Extracts

A detailed investigation was performed with the aim to find out the indigenous medicinal plants having anthelminthic action. Ten (10) indigenous medicinal plants were primarily selected and the ethanol extracts were prepared for anthelminthic trial and determination of anthelminthic properties in vitro and in vivo against the gastro-instestinal nematodes in goat during the period from July 2006 to December 2006. Screening of ethanol extracts of selected plants showed the anthelmintic activity against gastrointestinal nematodes at lower concentration (50 mg/ml). In vivo screening (by oral administration) of four plant extracts (ethanol) showed variable degree of efficacy in experimentally infected goats, as measured by faecal egg count reduction test. A relatively higher efficacy was recorded in ethanol extract of neem treated animals in comparison to other plants extracts. Ethanol extracts of korolla also showed significant efficacy. The results obtained in this study showed that ethanol extract of Labanga, Neem, Karolla and Pineapple at the dose of 100mg/kg showed a significant and potent antinematodal effect. These findings indicate that the adult gastrointestinal nematodes are more vulnerable to selected indigenous plants. Within these ten (10) plants 4 showed more than 70% efficacy at a concentration of 100mg/mkg. Key words: Medicinal plants, anthelmintics, nematodes, fecal egg count, goat doi: 10.3329/bjvm.v6i2.2333 Bangl. J. Vet. Med. (2008). 6 (2): 179-183

scholarly journals In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
X. De Jesús-Martínez ◽  
A. Olmedo-Juárez ◽  
J. Olivares-Pérez ◽  
A. Zamilpa ◽  
P. Mendoza de Gives ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Lethal Effect ◽  
Positive Control ◽  
Probit Analysis ◽  
Egg Hatching ◽  
Infective Larvae

The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows: 6.15, 3.12, 1.56, and 0.78 mg/mL to eggs and 150, 100, 75, and 50 mg/mL to larvae, respectively. Ivermectin (5 mg/mL) was used as positive control and 4% methanol and distilled water were used as negative controls. The data of ovicidal and larvicidal effect were analyzed with a completely randomized design through ANOVA analysis using the general linear model (GLM) and lethal concentrations (LC50 and LC90) were estimated through a Probit analysis using the SAS program. A clear ME increased concentration dependence effect was observed in the EHI and mortality tests. The highest activity of the methanolic extract was observed at the highest concentration (P < 0.05) to obtain a similar effect to the positive control (ivermectin), with LC50 = 78.38 and 0.00064 mg/mL and LC90 =235.63 and 0.024 mg/mL, respectively, for larvae and eggs. The results indicate that the C. coriaria fruit ME possesses in vitro ovicidal and larvicidal properties (gallotannins: methyl gallate) against H. contortus that needs to be investigated more in vivo for the control of gastroenteric nematodes in ruminants.

scholarly journals Uji Aktivitas Antelmintik Ekstrak Etanol Daun Ekor Naga (Rhaphidophora pinnata (L.) Schott) Secara In Vitro

2018 ◽  
Vol 1 (3) ◽  
pp. 090-094
Author(s):  
Masfria Masfria ◽  
Syaiful Amri Lubis ◽  
Lenny Lenny
Keyword(s):  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Screening ◽  
Pyrantel Pamoate ◽  
Parasitic Worm ◽  
Significant Difference ◽  
Post Hoc

Kecacingan merupakan permasalahan kesehatan di dunia. Munculnya strain cacing parasit yang resisten terhadap antelmintik menyebabkan pengobatan kecacingan menjadi sulit. Oleh karena itu perlu dilakukan pengujian sumber antelmintik baru. Pengujian ini dilakukan untuk mengetahui skrining simplisia dan ekstrak serta aktivitas antelmintik ekstrak etanol daun ekor naga (Rhaphidophora pinnata(L.)Schoot).Ekstrak didapatkan dengan mengekstraksi serbuk simplisia daun ekor naga (Rhaphidophora pinnata (L.) Schoot) dengan etanol 80% secara maserasi. Uji aktivitas antelmintik menggunakan cacing Pheretima hupiensis. Pirantel pamoat dengan konsentrasi 20 mg/mL digunakan sebagai kontrol positif. Aktivitas antelmintik ekstrak etanol daun ekor naga ditentukan berdasarkan waktu paralisis dan lisis Pheretima hupiensis. Hasil pengujian serbuk simplisia dan ekstrak etanol daun ekor naga mengandung senyawa alkaloid, flavonoid, glikosida, saponin, tanin, dan steroid/triterpenoid. Ekstrak etanol daun ekor naga (Rhaphidophora pinnata(L.) Schoot) memiliki aktivitas antelmintik terhadap cacing Pheretima hupiensis pada konsentrasi 30, 20, 15, 10, 5 mg/mL mampu membunuh cacing dengan waktu berturut-turut adalah 29,22; 46,80; 63,69; 82,66; 131,28 menit. Kelompok kontrol positif (pirantel pamoat) memiliki waktu kematian 107,64 menit sedangkan control negatif memberikan hasil negatif. Analisis statistika waktu kematian cacing dengan uji Tukey menunjukkan perbedaan secara signifikan dengan nilai p<0,05. Ekstrak etanol daun ekor naga (Rhaphidophora pinnata (L.) Schoot) mempunyai daya antelmintik terhadap cacing Pheretima hupiensis. Aktivitas antelmintik meningkat seiring dengan peningkatan konsentrasi ekstrak ethanol daun ekor naga.   Worms are a health problem in the world. The emergence of parasitic worm strains that are resistant to anthelmintics makes worm treatment difficult. Therefore it is necessary to find new anthelmintic agent. This study was carried out to determine the phytochemical screening pf dried powder materialand extract as well as the antelmintic activity of ethanol extract of ekor naga leaves (Rhaphidophora pinnata (L.) Schoot). The extract was obtained by maceration of dried powder of Ekor Naga (Rhaphidophora pinnata (L.) Schoot) leaves using ethanol 80%. The anthelmintic activity was evaluated on Pheretima Hupiensis. Pyrantel pamoate with a concentration of 20 mg/mL was used as a positive control. The anthelmintic activity of Ekor Naga leaves ethanol extract was performed based on time of paralysis and lyse of Pheretima Hupiensis. The phytochemical screening of dried powder material and extract of Ekor naga leaves ethanol extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, and steroid/triterpenoid. The ethanol extract of Ekor naga leaves (Rhaphidophora pinnata (L.) Schoot) displayed the anthelmintic activity on Pheretima Hupiensis with concentrations of 30, 20, 15, 10, 5 mg/mL that were able to destroy worms within 29,22; 46,80; 63,69; 82,66; 131,28 minutes, respectively. Positive control (pyrantel pamoate) induced worm mortality in 107,64 minutes meanwhile negative control did not induce mortality. Statistical analysis of worm mortality time by post hoc Tukey showed that there was a significant difference with p<0,05. Ethanol extract of Ekor naga (Rhaphidophora pinnata (L.) Schoot) leaves demonstrated anthelmintic activity on Pheretima Hupiensis. Anthelmintic activity increased with increasing concentrations of ethanol extract of Ekor naga leaves

scholarly journals UJI AKTIVITAS ANTELMINTIK EKSTRAK ETANOL DAUN SIRIH (Piper betle L.) TERHADAP CACING GELANG (Ascaris lumbricoides) SECARA IN VITRO

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 873
Author(s):  
Priskila Feicy Sumual ◽  
Widdhi Bodhi ◽  
Julianri Sari Lebang
Keyword(s):  
Ascaris Lumbricoides ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Piper Betle ◽  
Group Design ◽  
Significant Difference ◽  
Post Test

ABSTRACTGreen betel leaves are one of the native plants in Indonesia which are widespread in Manado, North Sulawesi. In green betel leaves plants, there are tannin compounds that can inhibit enzymes and interfere with the digestive metabolic processes of worms which can cause the death of worms. This study aims to determine the effect of green betel leaves ethanol extract on Ascaris lumbricoides worms. This research is a laboratory experiment with The post-test only with controlled group design. The tests used the betel leaves ethanol extract with concentrations of 5%, 10%, and 15% respectively. Worms was observed for 12 hours with intervals of 3 hours. The number of worm deaths was recorded every 3 hours and further, it was analysed with using the Kruskal Wallis test and it was continued with using the Mann Whitney test. Result showed that extract at concentration of 5% the number of worm deaths was 4 worms, a concentration of 10% was 7 worms, and at a concentration of 15% 9 worms. The statistical results showed that there was no significant difference between the number of worm deaths in the treatment group and negative control at p <0.05. The concentrations of 10 and 15 showed no significant difference with the positive control. It can be concluded that concentrations of 10% and 15% have the same anthelmintic activity but the best concentration is at a concentration of 10%. Keywords: Anthelmintic, Piper betle L., Ascaris lumbricoides   ABSTRAKDaun sirih hijau merupakan salah satu tanaman asli di Indonesia yang tersebar luas di kota Manado, Sulawesi Utara. Pada tumbuhan daun sirih hijau terdapat senyawa tanin yang mampu menghambat kerja enzim dan mengganggu proses metabolisme pencernaan pada cacing yang dapat menyebabkan kematian cacing. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun sirih hijau terhadap cacing Ascaris lumbricoides. Penelitian ini ialah eksperimental laboratorium dengan rancangan penelitian The post-test only with controlled group design. Pengujian dilakukan menggunakan ekstrak etanol daun sirih dengan konsentrasi 5%, 10%, dan 15%. Aktivitas cacing diamati selama 12 jam dengan interval waktu 3 jam. Jumlah cacing yang mati dicatat setiap 3 jam dan selanjutnya dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Whitney. Hasil pengujian menunjukkan pada konsentrasi 5% jumlah kematian cacing sebanyak 4 ekor, konsentrasi 10% sebanyak 7 ekor dan pada konsentrasi 15% sebanyak 9 ekor. Hasil statistik menunjukkan tidak ada perbedaan signifikan antara jumlah kematian cacing pada kelompok perlakuan dengan kontrol negatif pada p<0.05. Konsentrasi 10 dan 15 menunjukkan tidak ada perbedaan signifikan dengan kontrol positif. Dapat disimpulkan bahwa pada konsentrasi 10% dan 15% memiliki aktivitas antelmintik yang sama namun konsentrasi yang paling baik terdapat pada konsentrasi 10%. Kata kunci: Antelmintik, Piper betle L., Ascaris lumbricoides

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