scholarly journals Synthesis and cytotoxicity assay using Brine Shrimp Lethality Test of Cinchonidine Isobutyrate Ester

2017 ◽  
Vol 19 (1) ◽  
pp. 29-35
Author(s):  
Mario Mario ◽  
Puspa Dewi Lotulung ◽  
Gian Primahana ◽  
Sylvia Rizky Prima ◽  
Muhammad Hanafi
Keyword(s):  
Fatty Acid ◽  
Brine Shrimp ◽  
Anticancer Agent ◽  
Isobutyric Acid ◽  
Artemia Salina ◽  
Cytotoxicity Assay ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ft Ir ◽  
Pass Through

This research was aimed to synthesize cinchonidine isobutyrate ester and conduct a preliminary assay for anticancer agent using cytotoxicity assay to Artemia salina Leach larva, or also known as brine shrimp lethality test (BSLT). Cinchonidine, a compound that has quinoline rings and quiniclidine ring, is a quinine analogue and stereoisomer of cinchonine. Cinchonidine is predicted to have anticancer activity. Synthesized ester was aimed to gain higher lipophilicity. Higher lipophilicity makes it easier for the compund to pass through cell membrane. The esterification process used DMAP as a catalyst, DCC as an activator, and isobutyric acid as a carboxilyc acid. Isobutyric acid is a type of short chained fatty acid that usually acts as an anticancer prodrugs. The product is identified by ESI-MS, FT-IR, 1H-NMR, dan 13C-NMR. Ester cinchonidine isobutyrate is gelatinous and colourless with yield of 21,77%. BSLT result showed that cinchonidine isobutyrate ester had LC50 value of 75.16 ppm which was more toxic than cinchonidine that had LC50 value of 99.2 ppm. It was proved that higher lipophilicity could increase pharmacology activity

scholarly journals Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Elkawnie ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 67
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca ◽  
Fajar Indah Puspita Sari
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Positive Control ◽  
Acetone Extract ◽  
Antidiabetic Activity ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Ft Ir

Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.

scholarly journals Uji Toksisitas Ekstrak Batang Pinang Yaki (Areca vestiaria) pada Artemia salina Leach.

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Windy Astuti Tampungan
Keyword(s):  
Medicinal Plant ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Toxicity Level ◽  
Supernatant Solution

AbstrakEfek toksisitas pinang yaki (Areca vestaria) sebagai tumbuhan obat potensial perlu diuji untuk mengetahui ambang batas penggunaannya. Penelitian ini bertujuan untuk menguji tingkat toksisitas ekstrak batang pinang yaki terhadap larva Artemia salina Leach. dengan metode Brine Shrimp Lethality Test. Ekstrak diencerkan dengan konsentrasi 200, 400, 600 dan 800 ppm masing-masing untuk supernatan rendaman pertama (larutan A),supernatan rendaman ke dua (larutan B) dan supernatan rendaman ke tiga (larutan C) . Hasil penelitian menunjukkan bahwa ekstrak batang pinang yaki mempunyai efek toksisitas dengan nilai LC50 sebesar 398,28 ppm untuk larutan A, 390,84 ppm untuk larutan B dan 438,53 ppm untuk larutan C.Kata kunci: batang Areca vestaria,LC50, uji toksisitasAbstractToxicity effect of pinang yaki (Areca vestiaria) as potential medicinal plant should be evaluated to know its application threshold. This research aimed to evaluate the toxicity level of pinang yaki trunk extract on larve of Artemia salina Leach. using the method of Brine Shrimp Lethality Test. Extract was diluted to concentration of 200, 400, 600 and 800 ppm each for solution A (first soaking supernatant), solution B (second soaking supernatant)and solution C (third soaking supernatant). The result of this research showed that extract of pinang yaki trunk had a toxic characteristic, with LC50 value was 398,28, 390,84, and 438,53 ppm respectively for solution A, B and C.Keywords: LC50 , toxicity test, trunk of Areca vestaria

scholarly journals UJI TOKSISITAS AKUT INFUSA DAUN SIRSAK (Annona muricata) PADA LARVA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2020 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Arinta Mayang ◽  
Bilal S A Santoso
Keyword(s):  
Acute Toxicity ◽  
Medicinal Plant ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Annona Muricata ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Chemical Contents

ABSTRACTAnnona muricata is a family of Annonaceae that has been known as a medicinal plant. Sirsakleaves contain alkaloids, tannins, and several other chemical contents including acetogenin.The aim of this study was to determine the acute toxicity of the infusion of Sirsak leaves onthe Artemia salina larvae by the Brine Shirmp Lethality Test (BSLT) method. This experimentalstudy used 5 treatment concentrations (10 mg/L, 50 mg/L, 100 mg/L, 500 mg/L, 1000mg/L) and 1 negative control. All treatments were repeated 3 times. The number of Artemiasalina larvae used for each concentration was 10 larvae. The number of dead larvae wascounted after 24 hours of treatment. Based on probit analysis, the LC50 value of Sirsak leafinfusion was 38,73 mg/L. The conclusion of this research is the infusion of Sirsak leaves istoxic.Keywords: Sirsak leaves, toxicity, BSLTABSTRAKSirsak (Annona muricata) adalah keluarga Annonaceae yang telah dikenal sebagai tanamanobat. Daun Sirsak mengandung alkaloid, tanin, dan beberapa kandungan kimia lainnya termasukacetogenin. Tujuan dari penelitian ini adalah untuk menentukan toksisitas akut dari infusdaun Sirsak pada larva Artemia salina dengan metode Brine Shirmp Lethality Test (BSLT).Studi eksperimental ini menggunakan 5 konsentrasi perlakuan (10 mg/L, 50 mg/L, 100 mg/L,500 mg/L, 1000 mg/L) dan 1 kontrol negatif. Semua perawatan diulang 3 kali. Jumlah larvaArtemia salina yang digunakan untuk setiap konsentrasi adalah 10 larva. Jumlah larva matidihitung setelah 24 jam perlakuan. Berdasarkan analisis probit, nilai LC50 infus daun Sirsakadalah 38,73 mg/L. Kesimpulan penelitian ini adalah infus daun Sirsak bersifat toksik.Kata kunci: daun sirsak, toksisitas, BSLT

scholarly journals ANALISIS FITOKIMIA DAN PENENTUAN NILAI LC50 EKSTRAK METANOL DAUN LIWAS

2014 ◽  
Vol 14 (2) ◽  
pp. 125
Author(s):  
Gerry Sumihe ◽  
Max R. J. Runtuwene ◽  
Johnly A. Rorong
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRAK Penentuan nilai LC50 dari ekstrak metanol daun liwas menggunakan metode Brine Shrimp Lethality Test (BSLT) terhadap hewan indikator udang Artemia salina menunjukkan nilai LC50 dari  A. salina bersifat sangat toksik.Nilai LC50 yang didapatkan sebesar 15,696 mg/L. Nilai LC50 ditentukan dengan menggunakan metode SPSS 20.0.Ekstrak metanol daun liwas mengandung kandungan senyawa metabolit sekunder seperti flavonoid, tanin dan saponin sebagai hasil uji fitokimia. Kata kunci: BSLT, Daun Liwas, Metabolit Sekunder,LC50.   PHYTOCHEMICAL ANALYSIS AND DETERMINING LC50 VALUE OF LIWAS LEAVE METHANOL EXTRACT ABSTRACT Determination of LC50 of methanol extract of leaves liwas using Brine Shrimp Lethality Test (BSLT) against animal indicator shrimp Artemia salina showed LC50 values of  A. salina is highly toxic. LC50 values were obtained at 15.696 mg / L. LC50 values determined using SPSS 20.0. Liwas leaf methanol extract contains compounds secondary metabolites such as flavonoids, tannins and saponins as phytochemical test results. Keywords: BSLT, Leaves Liwas, Secondary Metabolites, LC50.

scholarly journals Isolasi dan Identifikasi Steroid dari Ekstrak N-Heksana Daun Afrika (Vernonia amygdalina Del.)

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 18-22
Author(s):  
Evi Mashunah ◽  
Erwin ◽  
Saibun Sitorus
Keyword(s):  
Data Analysis ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Test Results ◽  
Separation And Purification ◽  
Vernonia Amygdalina ◽  
Isolation And Identification ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ft Ir

Isolation and identification of steroids from the n-hexane fraction of Vernonia amygdalina Del. was carried out. Separation and purification were performed using flash chromatographic and gravity chromatography. Based on the results of phytochemical tests supported by UV and FT-IR data analysis, the isolate obtained was a steroid (sterol type). Toxicity test results using the Brine Shrimp Lethality Test (BSLT) were known that the steroid has moderate toxicity with an LC50 value of 48.39 ppm. Keywords: Vernonia amygdalina Del., Toxicity, BSLT, Sterol, Steroid

BRINE SHRIMP LETHALITY ASSAY AND LARVICIDAL ACTIVITY OF N-HEPTADECYL-N-(8-OCTA-1, 3-DIENE-6-YNYL)AMINE (C25H44N) ISOLATED FROM COMBRETUM MOLLE R.

2021 ◽  
Vol 5 (2) ◽  
pp. 166-172
Author(s):  
Abdu Zakari ◽  
Adoum Umar Al-Mubarak ◽  
Sani Aliyu
Keyword(s):  
Larvicidal Activity ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Disease Vector ◽  
Activity Test ◽  
Combretum Molle ◽  
Brine Shrimp Lethality Assay

The extracts of leaves of Combretum molle were tested against the larvae of Artemia salina, i.e. Brine Shrimp Lethality Test (BST) and Culexquinquefasciatus Say (filaria disease vector) i.e. Larvicidal Test, using crude ethanol, n-hexane, chloroform, ethyl acetate, and methanol extracts. The methanolic extract proved to be the most effective in inducing complete lethality at minimum doses both in the BST and the Larvicidal activity test. The LC50­ values obtained are 24.85 µg/ml and 0.4µg/ml respectively. The Bioactivity result was used to guide the column chromatography which afforded the pure compound ACM 2 which was not active in the BST with LC50 value of 1259 µg/ml but was active in the Larvicidal activity test with LC50 value 15.14 µg/ml.  ACM 2 was proposed to be N-heptadecyl-N-(8-octa-1, 3-diene-6-ynyl)amine (C25H44N).

UJI TOKSISITAS AKUT EKSTRAK ETANOL AKAR KB (Coptosapelta tomentosa Valeton ex K.Heyne) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 161
Author(s):  
Risa Supriningrum ◽  
Sapri Sapri ◽  
Vici Ali Pranamala
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

The roots of KB plants (Captosapelta tomentisa) is used empiricallyby  Dayak Kenyah community as a drug leukorrhea. Leukorrhea is one of the early symptoms of cervical cancer. Early methods to determine the LC50 value or toxicity of anticancer KB roots have not been reported. The aim of research is to determine the value of the toxicity of KB root. Research was conducted by an experimental study. Research stages include sample processing, extraction and acute toxicity test by Brine Shrimp Lethality Test (BSLT). BSLT testing to determine LC50 values with a concentration of 250 ppm, 300 ppm, 400 ppm, 450 ppm and a negative control. Analysis of the data used are probit analysis method. The results showed  the presence of flavonoids, alkaloids and saponins in of the ethanol extract of KB root . The test results obtained acute toxicity LC50 value is 299.226 mg / ml, so that the ethanol extract of roots KB potentially toxic to the larvae of Artemia salina Leach.

scholarly journals THE CYTOTOXIC ACTIVITY OF N-HEXANE EXTRACT OF KERSEN(muntingia calabura linn.) LEAVES USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD

2016 ◽  
Vol 16 (2) ◽  
pp. 37-44
Author(s):  
Irma Sari ◽  
Titania Miranda ◽  
Sadli Sadli
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Hexane Extract ◽  
Water Soluble ◽  
Extractive Content ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Muntingia Calabura

The cytotoxic activity experiment of n-hexane extract of kersen (Muntingia calabura Linn.) leaves has been carried out using the Brine Shrimp Lethality Test (BSLT) method. The purpose of this research is to identify the secondary metabolites, extract characterize, and determine the LC50 value of the extract against larvae shrimp Artemia salina Leach. Screening result showed that n-hexane extract of kersen leaves contain triterpenoid. Extract characterization showed the results water content of 2.59±0.18 %w/w, water soluble extractive content of 1.9%±0.19 %w/w, the ethanol soluble extractive content of 17.41±1,87 %w/w and total ash value 0.25% w/w. Extract showed LC50 value is 278,72 ppm were calculated by probit analysis. The LC50 value indicated that the n-hexane extract of kersen leaves potentially has cytotoxic activity.

scholarly journals TOKSISITAS AKUT KOMBUCHA DAUN TIN (Ficus carica) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

2020 ◽  
Vol 3 (1) ◽  
pp. 11
Author(s):  
Uswatun Uswatun ◽  
Ernanin Dyah Wijayanti
Keyword(s):  
Secondary Metabolites ◽  
Acute Toxicity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Ficus Carica ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRACTFig leaves (Ficus carica) contain secondary metabolites such as flavonoids, tannins, alkaloids,saponins and triterpenoids, which potentially toxic in a certain amount. Fermentation of figleaves using kombucha produce healthy drink with various bioactivities. The aim of this researchwas to observe acute toxicity of fig leaves kombucha using Brine Shrimp LethalityTest (BSLT) method. Toxixity test againts Artemia salina Leach larvae using 7 variations infig leaves kombucha concentration from 100 ppm to 30000 ppm. The results showed that figleaves kombucha LC50 value of 139,99 ppm, so that fig leaves kombucha is potentially toxic.Key words: acute toxicity, BSLT, fermentation, fig leavesABSTRAKDaun tin (Ficus carica) mengandung metabolit sekunder antara lain flavonoid, tanin, alkaloid,saponin dan triterpenoid yang berpotensi toksik dalam jumlah tertentu. Fermentasi dauntin oleh kultur kombucha menghasilkan minuman kesehatan dengan berbagai bioaktivitas.Penelitian ini bertujuan untuk mengetahui toksisitas akut kombucha daun tin menggunakanmetode Brine Shrimp Lethality Test (BSLT). Pengujian toksisitas terhadap larva Artemia salinaLeach menggunakan 7 variasi konsentrasi kombucha daun tin antara 100 ppm sampai30000 ppm. Hasil penelitian menunjukkan nilai LC50 kombucha daun tin sebesar 139,99ppm, sehingga disimpulkan bahwa kombucha daun tin berpotensi toksik.Kata kunci: BSLT, daun tin, fermentasi, toksisitas akut

scholarly journals Uji Toksisitas Akut Kulit Kentang (Solanum tuberosum L.) Terhadap Larva Artemia salina Leach Dengan Metode BSLT (Brine Shrimp Lethality Test)

2019 ◽  
Vol 2 (2) ◽  
pp. 10
Author(s):  
Baterun Kunsah
Keyword(s):  
Brine Shrimp ◽  
Solanum Tuberosum L ◽  
Artemia Salina ◽  
The Body ◽  
Control Group ◽  
Brine Shrimp Lethality ◽  
Advantages And Disadvantages ◽  
Lc50 Value ◽  
Body Tissues ◽  
Potato Skin

Tanggal Submit:28 Januari 2019 Tanggal Review:30 April 2019 Tanggal Publish Online:17 Mei 2019 Potatoes are one of the vegetables that contain substances that are important for the formation of body tissues, such as protein and fat. Potatoes also form red blood cells or hemoglobin (Ca, P and Fe). In Indonesia tend to process potatoes using only meat. The skin is removed, even though the nutritional content of the skin is five times greater than the meat. Potato peels that supply querestin, antioxidants and flavonoids that act as free radical acceptors. (Free radicals are reactive molecules that cause damage to the body that can lead to diseases such as heart disease and cancer) in the skin of potatoes found also effective antioxidants, chlorogenic acid. Potato skin is also proven to increase hemoglobin levels (Khomsan, A, 2009). However, that does not mean potato skin does not have adverse side effects, if the use is not appropriate. In order to use it optimally, you need to know enough information about the advantages and disadvantages and the possibility of abuse. However, the level of toxicity from potato skin is still unknown. Brine Shrimp Lethality Test (BSLT) is one of the oxicity test methods that is widely used in tracing bioactive compounds that are toxic from natural materials. This research is an experimental research with post test-only control group design approach. Treatment with giving potato skin juice to Artemia salina Leach larvae with the aim to determine the potential of plant biological activity based on the toxicity of secondary metabolites contained in it, and at the same time as the initial screening test of anticancer activity of chemical compounds in potato skin juice. The results showed the LC50 value was at a concentration of 0.5% to 1% and based on the results of SPSS 21 analysis with probit analysis obtained LC50 price data of 0.746 gr / ml (7460 μgr / ml), based on the results of research and data analysis it can be concluded that potato skin has an LC50 value at a concentration of 0.746 gr / ml (7460 µgr / ml). With an LC50 price of more than 1000 µg / ml according to the BST method the potato skin does not have the potential for toxicity so the skin of the potato has the potential as an alternative to increasing hemoglobin levels.Keywords: potato skin, toxicity, BSlT

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