REPRODUCTIVE ASPECTS OF THE TROPICAL ABALONE, HALIOTIS ASININA, FROM SOUTHERN LOMBOK WATERS, INDONESIA

The main objective of this study was to determine reproductive strategy including major spawning season of tropical abalone (Haliotis asinina) from southern Lombok waters, Indonesia. Gonad bulk index, maturity stages and oocyte size frequency distribution were applied to document gonadal development and major spawning season. The results of this study show that H. asinina in southern Lombok waters displayed year-round spawning with a high proportion occurring in August - November, when gonad index dropped to a lower level and high proportion of partly spawned and/or spent ovaries occurred in the population. Gonad development and spawning in males and females occurred synchronously. The correlation between gonadal development and environmental factors are described and discussed.

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On the reproduction of the Mediterranean keystone limpet Patella rustica: histological overview

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315414000976 ◽

2014 ◽

Vol 94 (8) ◽

pp. 1651-1660 ◽

Cited By ~ 4

Author(s):

Ivana Prusina ◽

Daria Ezgeta-Balić ◽

Stijepo Ljubimir ◽

Tatjana Dobroslavić ◽

Branko Glamuzina

Keyword(s):

Reproductive Cycle ◽

Shell Length ◽

Histological Analysis ◽

Developmental Stages ◽

Gonad Development ◽

Gonad Index ◽

Keystone Species ◽

Males And Females ◽

Sexually Mature ◽

High Shore

The reproductive cycle of the high shore limpet Patella rustica is described based on histological analysis as the primary method of staging gonad development. Sex-ratios, shell length at sexual maturity, gonad developmental stages, mean gonad index and oocyte size were investigated. Males and females were found to differ in size distribution, with females becoming more prevalent from ~28 mm onwards. The estimated shell length at which 50% of males were sexually mature was 13.1 mm. Patella rustica has only one reproductive cycle per year with a spawning peak between November and December for both sexes, and gonad redevelopment from January. First data on the size–frequency analysis of oocytes for this species are also presented, concurring with the qualitative analysis of the gonad developmental stages. This study presents updated information on the reproductive cycle of this keystone species and provides the first account of the reproductive biology of P. rustica in the Adriatic Sea.

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Oocyte development and fecundity type of the Brazilian Snapper Lutjanus alexandrei Moura & Lindeman, 2007 (Perciformes: Lutjanidae)

Brazilian Journal of Biology ◽

10.1590/1519-6984.14714 ◽

2016 ◽

Vol 76 (1) ◽

pp. 126-135

Author(s):

C. A. F. Fernandes ◽

P. G. V. Oliveira ◽

C. H. B. Oliveira ◽

F. H. V. Hazin ◽

P. Travassos

Keyword(s):

Frequency Distribution ◽

Spawning Season ◽

Oocyte Development ◽

Growth Stages ◽

Oocyte Size ◽

Oocyte Growth ◽

Size Frequency Distribution ◽

Spawning Period ◽

Size Frequency ◽

Oocyte Stage

Abstract Lutjanid species exhibit multiple spawning behaviour during an extended spawning season in warm months, asynchronous oocyte development and indeterminate fecundity. Although early studies have contributed to knowledge of the reproductive cycle of many species within the group, they have not considered aspects about the number of cortical alveoli oocyte stage throughout maturity phases along spawning season. The latter aspect is also considered very important to confirm indeterminate fecundity hypothesis. In the present study, were analyzed 154 Brazilian snapper Lutjanus alexandrei female gonads obtained from artisanal fisheries in Pernambuco State (Brazil) between October 2010 and March 2011. Were measured oocyte size frequency distribution for maturity phases (developing, spawning capable and actively spawning), and oocyte development stage (unyolked oocytes, cortical alveoli, primary, secondary and tertiary vitellogenic oocytes and hydrated oocytes), and also the oocyte stage frequency during spawning season. The frequency of cortical alveoli oocyte stage was constantly found in the spawning period (>37%), showing slight variation throughout maturity phases. The absence of gap in the oocyte size frequency distribution between primary and secondary oocyte growth stages during spawning season is a strong indicator of continuous oocyte recruitment from reserve stocks. In addition, co-occurrence of tertiary vitellogenic oocytes, hydrated oocytes, post-ovulatory follicles and yellow-brown bodies in the histological sections of ovaries reinforce indeterminate fecundity hypothesis.

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Reproductive seasonal periodicity of the endemic Bermuda killifish Fundulus bermudae in an anchialine pond

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315407053982 ◽

2007 ◽

Vol 87 (3) ◽

pp. 797-800

Author(s):

Mark Outerbridge ◽

John Davenport ◽

Anne F. Glasspool

Keyword(s):

Gonad Development ◽

Laboratory Analysis ◽

Spawning Season ◽

Early Winter ◽

Seasonal Periodicity ◽

Steady Rate ◽

Males And Females ◽

Reproductive Cycles

A total of 245 individuals from a population of the endemic Bermuda killifish Fundulus bermudae in an isolated anchialine pond (Mangrove Lake) were trapped between November 2004 and November 2005. Laboratory analysis of gonad development allowed determination of the seasonal reproductive cycles of both females and males. A distinctive annual pattern was evident, with female and male gonadal cycles synchronous throughout the study period. The results indicate that the F. bermudae males and females began their spawning season in February, but reached primary peaks in May and June respectively. Gonadal indices abruptly fell after June and continued to fall at a steady rate until September, marking the end of the spawning season. Gonad recrudescence, as indicated by basal gonad indices, occurred in September and lasted throughout the autumn and early winter months.

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The dual nature of obesity in metabolic programming: quantity versus quality of adipose tissue

Clinical Science ◽

10.1042/cs20201028 ◽

2020 ◽

Vol 134 (18) ◽

pp. 2447-2451

Author(s):

Anissa Viveiros ◽

Gavin Y. Oudit

Keyword(s):

Adipose Tissue ◽

Environmental Factors ◽

Maternal Obesity ◽

Metabolic Programming ◽

Dual Nature ◽

Prevalence Of Obesity ◽

Adipose Tissue Volume ◽

Males And Females ◽

Metabolically Healthy

Abstract The global prevalence of obesity has been rising at an alarming rate, accompanied by an increase in both childhood and maternal obesity. The concept of metabolic programming is highly topical, and in this context, describes a predisposition of offspring of obese mothers to the development of obesity independent of environmental factors. Research published in this issue of Clinical Science conducted by Litzenburger and colleagues (Clin. Sci. (Lond.) (2020) 134, 921–939) have identified sex-dependent differences in metabolic programming and identify putative signaling pathways involved in the differential phenotype of adipose tissue between males and females. Delineating the distinction between metabolically healthy and unhealthy obesity is a topic of emerging interest, and the precise nature of adipocytes are key to pathogenesis, independent of adipose tissue volume.

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IMPLANTATION OF LHRH-a HORMONE TO STIMULATION OF GONAD DEVELOPMENT OF HUMPBACK GROUPER SECOND GENERATION ( F-2 )

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v5i2.7561 ◽

2013 ◽

Vol 5 (2) ◽

Author(s):

Tridjoko Tridjoko

Keyword(s):

Body Weight ◽

Second Generation ◽

Gonad Development ◽

Gonadal Development ◽

Oocyte Diameter ◽

2Nd Generation ◽

Length Range ◽

Concrete Tank ◽

Implant Treatment ◽

Stimulation Of

This study aimed to determine the effect of LHRH-a hormone implantation on gonadal development of humpback grouper, Cromileptes altivelis 2nd generation (F-2). Individuals used were 20 female grouper fish with weight range of 600-800 gram/fish and total length range of 28.0-30.0 cm. The treatment used 5 fish each with tagging. Grouper fishes were stocked into the 75 m3 circular concrete tank. Hormone of LHRH-a with dosage of 50 μg/kg body weight was used as implant treatment of (A) 1 time,, (B) 2 times, (C) 3 times, and (D) without implant. The results showed that implantation of LHRH-a hormone were significantly stimulate gonad development of humpback grouper second generation (F-2). Treatment of 3 times implant every month showed the best results produced oocyte diameter up to 480 μm, while the control of oocyte diameter only reached <400 μm. Keywords: LHRH-a hormone, implantation, Humpback grouper, gonad development

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Cuttlebone morphometrics and sex identification of Sepia bertheloti (d’Orbigny, 1835) from the central-east Atlantic

Helgoland Marine Research ◽

10.1186/s10152-019-0531-0 ◽

2019 ◽

Vol 73 (1) ◽

Author(s):

Airam Guerra-Marrero ◽

David Jiménez-Alvarado ◽

Vicente Hernández-García ◽

Leticia Curbelo-Muñoz ◽

José Juan Castro-Hernández

Keyword(s):

Morphometric Analysis ◽

Growth Pattern ◽

Gonadal Development ◽

Sex Identification ◽

East Atlantic ◽

Subsequent Increase ◽

Northwest Africa ◽

Males And Females

Abstract Analysis of 322 cuttlebones of Sepia bertheloti caught in the waters of the Northwest Africa showed significant differences in growth between males and females. Morphometric analysis revealed a relatively different cuttlebone growth pattern between sexes, with males presenting faster growth in length to reach larger sizes and females displaying greater growth in width. This difference in cuttlebone growth is related to female gonadal development and the subsequent increase of the paleal cavity, providing more support and space to accommodate a higher number of oocytes.

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REPRODUCTIVE ASPECTS OF SCALLOPED PERCHLET, Ambassis nalua (HAMILTON, 1822) IN PABEAN BAY INDRAMAYU, WEST JAVA

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.28930/jitkt.v9i1.17921 ◽

2017 ◽

Vol 9 (1) ◽

pp. 113

Author(s):

Nisha Desfi Arianti ◽

M.F. Rahardjo ◽

Ahmad Zahid

Keyword(s):

Sex Ratio ◽

Sampling Period ◽

Spawning Season ◽

West Java ◽

Fish Samples ◽

Length Range ◽

Males And Females ◽

Mature Fish ◽

First Maturity ◽

Somatic Index

ABSTRACTScalloped perchlet, Ambassis nalua, is one of fishes in Pabean Bay, Indramayu. This research aims to determine the reproductive aspects of Ambassis nalua in Pabean Bay Indramayu including sex ratio, spawning season, first maturity, fecundity and spawning pattern. Fish samples were collected by trap net and trammel net at three sites in Pabean Bay from April to October 2015. A total of 424 of A. nalua were caught, consist of 114 males and 310 females; with total length ranged from 38.04 to 112.63 mm and total weight ranged from 0.37 to 25.44 g. Sex ratio of mature fish was 1:1.9. The mature males and females were found in every month of sampling period. The gonado-somatic index (GSI) ranged from 0.40 to 0.83 and 2.36 to 4.54 for male and female, respectively. The peak of spawning season was found in September. The first maturity (Lm50) for male and female were 79.17 mm and 91.25 mm, respectively. The fecundity varied from 3,451–32,465 eggs. Egg diameter distribution shows that spawning pattern of A. nalua was batch spawner. Keywords: Ambassis nalua, spawning season, sex ratio, reproduction

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Effects of gonadotropin-releasing hormone analog (GnRHa) immunization on the gonadal transcriptome and proteome of tilapia (Oreochromis niloticus)

10.21203/rs.3.rs-18227/v1 ◽

2020 ◽

Author(s):

Rui Wang ◽

Luting Wen ◽

Huawei Ma ◽

Huizan Yang ◽

Min Lv ◽

...

Keyword(s):

Differentially Expressed Genes ◽

Expression Patterns ◽

Gonad Development ◽

Gonadal Development ◽

Gonadotropin Releasing Hormone ◽

Differentially Expressed ◽

Receptor Interaction ◽

Releasing Hormone ◽

Data Independent Acquisition ◽

Single Organism

Abstract Background: Gonadotropin releasing hormone (GnRH) plays an important role in the regulation of vertebrate reproduction. Studies have shown that immunization against GnRHa can induce sexually sterile tilapia. To explore the mechanism behind this, in this study, RNA-seq and data-independent acquisition (DIA) techniques were used to study the transcriptome and proteome of the gonad of tilapia immunized with GnRHa. Results : 644 differentially expressed genes (80 upregulated and 564 downregulated) and 1150 differentially expressed proteins (351 upregulated and 799 downregulated) were identified. There were 209 genes with consistent differential expression patterns in the transcriptomic and proteomic analyses, of which 9 were upregulated and 200 downregulated, indicating that the gonad gene expression was inhibited by GnRHa immunization. The downregulated genes were particularly involved in the functions of single-organism process, binding, cellular process, metabolic process and catalytic activity, and associated with the pathways including ECM–receptor interaction, focal adhesion, cardiac muscle contraction and oxidative phosphorylation. The expression of six differentially expressed genes involved in the GnRH signaling pathway was all downregulated. In addition, several important functional genes related to gonadal development after GnRHa immunization were screened. Conclusions: This study confirmed the expression of corresponding genes was affected by GnRHa on the gonad development in tilapia at the molecular level, and laid a foundation for elucidating the mechanism of GnRHa immunization.

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A Comparative Study of Palmar Dermatoglyphics in Thakurs and Lodhis of Bakshi Ka Talab Lucknow, Uttar Pradesh

SRI JNPG COLLEGE REVELATION A JOURNAL OF POPULAR SCIENCE ◽

10.29320/sjnpgrj.v2i01.11029 ◽

2018 ◽

Vol 2 (01) ◽

Author(s):

Preeti Gupta ◽

Udai Pratap Singh

Keyword(s):

Environmental Factors ◽

Comparative Study ◽

Uttar Pradesh ◽

White Paper ◽

The Other ◽

Main Line ◽

Males And Females ◽

Index Value ◽

Two Populations ◽

Dermatoglyphic Traits

Dermatoglyphic characters vary widely and are unaffected by age, gender and least by environment. Unlike configurations and their distinctiveness with every individual, it also shows bilateral, bisexual and population variations. Palm prints once created are unchangeable throughout life and are unaffected by environmental factors. This paper is based upon the palmar dermatoglyphic. The study reports on bisexual and bilateral palm prints among Lodhis and Thakurs of Rudahi village of Bakshi Ka Talab, Lucknow District. The palmar dermatoglyphic traits have been analyzed among the two populations which are Main line Formula, Endings of main lines D and A, Position of Axial triradius, Angle atd, Main line Index, Hypothenar, Thenar/ Ist Interdigital area, IInd Interdigital area, IIIrd Interdigital area, IVth Interdigital area, and Palmar Ridge Counts. The prints have been obtained by using the duplicating ink method on white paper. On the perusal of Principal Main line Formula among Lodhis and Thakurs, it has been noticed that the frequency is more in Lodhi males than females in formula 11.9.7. On the other hand, the frequency of Thakur males is more than Thakur females in formula 9.7.5. The Ending of Main Line D in position 11 as clearly indicated in Lodhis is higher in both the genders in comparison to Thakurs, while the position 9 indicates highest frequency in Thakur females. The Ending of main line A at position 5’ has higher frequency in Lodhi males. The Main line index value 16 is observed higher in Lodhis in comparison to Thakurs. Higher frequency has been observed in Lodhis than Thakurs in Axial triradius in modal type‘t’. The range of Angle atd is observed to have higher frequency in 36º- 40º among the Lodhis as compared to Thakurs. The palmar ridge counts mean, is found higher in Lodhi females and Thakur males. Thus this paper shows the similarity and dissimilarity among the males and females of Thakurs and Lodhis in Palmar dermatoglyphics.

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Mouse Gonad Development in the Absence of the Pro-Ovary Factor WNT4 and the Pro-Testis Factor SOX9

Cells ◽

10.3390/cells9051103 ◽

2020 ◽

Vol 9 (5) ◽

pp. 1103

Author(s):

Furong Tang ◽

Nainoa Richardson ◽

Audrey Albina ◽

Marie-Christine Chaboissier ◽

Aitana Perea-Gomez

Keyword(s):

Granulosa Cells ◽

Gonad Development ◽

Supporting Cell ◽

Gonadal Development ◽

Ovary Development ◽

Double Knockout ◽

Cell Identity ◽

Knockout Mouse Model ◽

Steroidogenic Cell ◽

Male Specific

The transcription factors SRY and SOX9 and RSPO1/WNT4/β-Catenin signaling act as antagonistic pathways to drive testis and ovary development respectively, from a common gonadal primordium in mouse embryos. In this work, we took advantage of a double knockout mouse model to study gonadal development when Sox9 and Wnt4 are both mutated. We show that the XX gonad mutant for Wnt4 or for both Wnt4 and Sox9 develop as ovotestes, demonstrating that ectopic SOX9 function is not required for the partial female-to-male sex reversal caused by a Wnt4 mutation. Sox9 deletion in XY gonads leads to ovarian development accompanied by ectopic WNT/β-catenin signaling. In XY Sox9 mutant gonads, SRY-positive supporting precursors adopt a female-like identity and develop as pre-granulosa-like cells. This phenotype cannot be fully prevented by the deletion of Wnt4 or Rspo1, indicating that SOX9 is required for the early determination of the male supporting cell identity independently of repressing RSPO1/WNT4/β-Catenin signaling. However, in XY Sox9 Wnt4 double mutant gonads, pre-granulosa cells are not maintained, as they prematurely differentiate as mature granulosa cells and then trans-differentiate into Sertoli-like cells. Together, our results reveal the dynamics of the specific and independent actions of SOX9 and WNT4 during gonadal differentiation: SOX9 is essential in the testis for early specification of male-supporting cells whereas WNT4 functions in the ovary to maintain female-supporting cell identity and inhibit male-specific vascular and steroidogenic cell differentiation.

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