scholarly journals Vitamin E effect on osmotic fragility in β thalassemia major

2014 ◽  
Vol 54 (5) ◽  
pp. 280
Author(s):  
Agus Fitrianto ◽  
Moedrik Tamam ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Vitamin E ◽  
Osmotic Fragility ◽  
Thalassemia Major ◽  
Post Treatment ◽  
Control Group ◽  
Erythrocyte Osmotic Fragility ◽  
Group I ◽  
Group Ii ◽  
Pre Treatment ◽  
Vitamin E Supplementation

Background Blood transfusion remains the main therapy for anemia in β thalassemia major patients. However, frequent transfusions can cause oxidative stress in response to iron overload. Vitamin E is considered to be the best lipid-soluble exogenous antioxidant in humans. It can protect phospholipid membrane from peroxidarion. Erythrocyte osmotic fragility is a useful test to assess for the improvement of red blood cells in thalassemia patients after vitamin E supplementation.Objective To investigate the effect of vitamin E for improving erythrocyte osmotic fragility in β thalassemia major and for decreasing the need for frequent transfusions.Methods T his was a double blind placebo controlled randomized clinical trial on children aged 2-14 years with thalassemia major who received frequent blood transfusions. Fifty subjects were divided into 2 groups: group I with vitamin E supplementation and group II with placebo, as a control group, for a period of 1 month. Pre- and post-treatment data on erythrocyte osmotic fragility and hemoglobin level were analyzed with non-paired T-test.Results Improved erythrocyte osmotic fragility was found: in group I, pre-treatment 31.59 (SD 6.342)% to post-treatment 38.08 (SD 7.165)%, compared to the control group pre-treatment 34.40 (SD 6.985)% to post-treatment 29.26 (SD 9.011)% (P=0.0001). Comparison of the mean delta Hb level in group  I was 0.94 (SD 0.605) gr% and that of group II was - 0.23 (SD 1.199) gr% (P=0.0001).Conclusion Vitamin E supplementation improves erythrocyte fragility and Hb level in β thalassemia major pediatric patients.

scholarly journals Acute Toxicity and Erythrocyte Osmotic Fragility Studies of Methanol Leaf Extract of Asystasia vogeliana in Rats

2020 ◽  
pp. 18-28
Author(s):  
Hillary Emeka Ugwuanyi ◽  
Patrick Emeka Aba ◽  
Samuel Chukwuneke Udem ◽  
Innocent Ifeanyi Madubuinyi
Keyword(s):  
Body Weight ◽  
Osmotic Fragility ◽  
Group Iv ◽  
The Body ◽  
Total Phenolic ◽  
Control Group ◽  
Acute Administration ◽  
Erythrocyte Osmotic Fragility ◽  
Group I ◽  
Group Ii

Aim: To investigate the phytoconstituents of methanol and petroleum leaf extracts of Asystasia vogeliana (MLEAV and PLEAV), the median lethal dose (LD50) and the effects of MLEAV on body weight, organosomatic indices in vital organs and erythrocyte membrane of Albino Wistar rats during sub-acute administration. Place and Duration of Study: Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, in 2017. Methodology: The crude extracts of MLEAV and PLEAV were used in determining the qualitative and quantitative analyses. The rats were assigned into four groups and dosed orally with distilled water (0.5 ml/100 g) as Group I, 62.5 mg/kg MLEAV (Group II), 125 mg/kg MLEAV (Group III) and 250 mg/kg MLEAV (Group IV) once daily for 28 days. Blood samples were collected from all the rats via the medial canthus into EDTA bottles for erythrocyte osmotic fragility (EOF) study on day 29. Relative organ-body weight indices of vital organs (spleen, heart, liver and kidney) were also evaluated. Results: MLEAV and PLEAV showed the presence of saponins, flavonoids, phenols, alkaloids, anthraquinones and steroids. The total phenolic contents of MLEAV (3704.30 ± 44.00) significantly increase (P = 0.000) when compared with PLEAV (1349.46 ± 35.25). The LD50 of MLEAV is above 5000 mg/kg. There were significant (P < 0.05) decreases in the body weights of rats in Groups III and IV from 3rd to 4th week when compared with their baseline weights. There were significant (P < 0.05) increases in the relative spleen-body weight in Group IV when compared with other groups. There was no significant change (P > 0.05) in the hemolysis of rats in Group II when compared with the control group at 0.9% NaCl concentration. Conclusions: The findings reveal that MLEAV showed better antioxidant capacity than PLEAV, and that 62.5 mg/kg of MLEAV is safe during the sub-acute administration.

scholarly journals COMPARATIVE STUDY BETWEEN THE EFFECT OF HISTAMINE RECEPTOR ANTAGONISTS OF TYPE II (FAMOTIDINE) AND PROTON PUMP INHIBITORS (OMEPRAZOLE) ON THE EFFICACY OF CALCIUM CARBONATE AS PHOSPHATE BINDER IN HAEMODIALYSIS PATIENT

2017 ◽  
Vol 9 (8) ◽  
pp. 10
Author(s):  
Hossam Aboelyazeed ◽  
Sahar El-haggar ◽  
Kamal Okasha
Keyword(s):  
Calcium Carbonate ◽  
Serum Calcium ◽  
Phosphate Binder ◽  
Renal Dialysis ◽  
University Hospital ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Pre Treatment

Objective: The purpose of this study was to compare the effect of famotidine versus omeprazole on the efficacy of calcium carbonate as a phosphate binder in the hemodialysis patient.Methods: From February 2014 to June 2014 a total number of 64 patients of both sexes were recruited from the department of renal dialysis, Tanta University Hospital, Egypt. Patients categorized into 3 groups. Group I (control group) consisted of 20 Patients (10) females and (10) males take calcium carbonate (caco3) (2.5–4 g/d) only, Group II consisted of 21 Patients (13) females and (8) males take the same dose of caco3 with famotidine 10 mg/d and Group III consisted of 23 Patients (8) females and (15) male take the same dose caco3 with omeprazole 20 mg/d.Results: All data are expressed as the mean±SD. Group II showed a significant increase (p<0.05) in serum phosphorus at 3rd mo with significant decreased (p<0.05) in serum calcium comparing with pre-treatment. Group III showed no significant change (p>0.05) in serum calcium, phosphorus and parathyroid hormone (PTH) comparing with pre-treatment. Both groups (II and III) showed a significant decrease in alkaline phosphatase (ALP) (p<0.05).Conclusion: Co-administration of famotidine with calcium carbonate aggravates hyperphosphatemia and this may increase the incidence of complications. The efficacy of calcium carbonate as a phosphate binder was not affected by co-administration of omeprazole.

Efficacy of the new Direct Acting Antiviral drugs in the treatment of Thalassemic HCV patients

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Sherif A Megahed ◽  
Maha A El Touny ◽  
Iman A Ragab ◽  
Ossama A Ahmed ◽  
Shereen A Ibrahim ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Side Effects ◽  
Thalassemia Major ◽  
Post Treatment ◽  
Hcv Infection ◽  
Combination Regimen ◽  
Group I ◽  
Transfusion Requirements ◽  
Group Ii ◽  
Cessation Of Treatment

Abstract Background and Objectives B-thalassemia major patients are susceptible to Hepatitis C Virus (HCV) infection owing to life-long dependency for blood-transfusion. Moreover, this patient population is at risk of progression of liver fibrosis or development of cirrhosis as a consequence of both iron overload and HCV infection. However, patients with haemoglobinopathies and CHC have been excluded from the major clinical trials that led to the approval of DAAs, Hence, at present, limited experience is available regarding the safety and efficacy of DAAs in this population which is traditionally considered difficult to treat. Hence, this study was carried out to evaluate efficacy and safety of the combination regimen of sofosbuvir and daclatasvir for HCV infection in B-thalassemia major patients. Methods This study was conducted on 200 subjects divided into two groups, first group contains150 HCVThalassemic patients while the second group contains 50 HCV only patients. Each group was classified into easy to treat or difficult to treat and received sofosbuvir 400mg + daclatasvir 60mg once/day for the duration of 12 or 24 weeks according to the NCCVH Hepatitis C treatment protocol 2015. Sustained virological response at post-treatment week-12 (SVR-12) was defined as negative HCV-RNA at week-12 post treatment. Results In group (I), successful SVR was achieved in all patients (100%) in subgroup (Ia) while in subgroup (Ib) 12 patients didn’t achieve SVR (15.38%), 4 patients stopped due to side effects(5.13%) and 62 patient achieved SVR (79.49) with overall successful SVR of 134 out of 150 HCV-Thalassemic patients in group (I) (89.33%). in group (II), 2 patients didn’t achieve SVR (5.71%)and 33 patients achieved SVR (94.29%) in subgroup (IIa) while in subgroup (IIb) 2 patients didn’t achieve SVR (13.33%) and 13 patients achieved SVR (86.67%) with overall successful SVR of 46out of 50 HCV only patients in group (II) (92%). few patients suffered from minor side effects that didn’t require cessation of treatment but 4 patients developed major side effects in group (Ib) that required cessation of treatment. There were marked improvement in liver enzymes, Fib4 score, hemoglobin level and transfusion requirements in HCVThalassemic group after treatment. Conclusion A combination of sofosbuvir and daclatasvir is an efficacious and tolerable treatment regimen with negligible side effects for patients with thalassemia major and HCV infection.

Evaluating the Effect of Probiotic Containing Milk on Salivary mutans streptococci Levels

2012 ◽  
Vol 37 (1) ◽  
pp. 9-14 ◽  
Author(s):  
A Juneja ◽  
A Kakade
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Post Treatment ◽  
Mutans Streptococci ◽  
Control Group ◽  
P Value ◽  
Group I ◽  
Probiotic Lactobacillus ◽  
Group Ii ◽  
The Difference

Purpose: To evaluate the changes in mutans streptococci counts in saliva after short term probiotic intervention and its delayed effects on salivary mutans streptococci count. Methods: 40 children in the age group of 12-15 years with medium to high caries activity were randomly divided into Group I Control (plain milk group) and Group II Experimental (probiotic supplemented milk group). Duration of the study was 9 weeks; which was evenly divided into three phases: baseline, intervention and post-treatment period; each phase consisting of three weeks. After baseline period of 3 weeks, children in group I were given plain milk and in group II milk containing probiotic Lactobacillus rhamnosus hct 70 for 3 weeks; followed by a 3 weeks follow up period. After every phase saliva samples were collected to estimate salivary mutans streptococci counts. Results: The difference in the post follow up mutans streptococci count of group I and group II, was highly significant with p value &lt; 0.001. In the control group, the difference in the mean salivary baseline, post treatment and post follow up mutans streptococci counts was not statistically significant (p = 0.001). In the experimental probiotic group, the difference in mean salivary baseline, post treatment and post follow up mutans streptococci counts was statistically highly significant ( p = 0.000, p ≤ 0.001). Conclusions: Statistically significant reduction in salivary mutans streptococci counts immediately after consumption of probiotic Lactobacillus rhamnosus hct 70 containing milk suggest a beneficial effect of probiotic Lactobacillus rhamnosus hct 70 in the prevention of dental caries.

Ameliorative effect of vitamin C on chronic chlorpyrifos-induced erythrocyte osmotic fragility in Wistar rats

2010 ◽  
Vol 30 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Suleiman F Ambali ◽  
Joseph O Ayo ◽  
Samuel A Ojo ◽  
King AN Esievo
Keyword(s):  
Vitamin C ◽  
Lethal Dose ◽  
Osmotic Fragility ◽  
Erythrocyte Membranes ◽  
Control Group ◽  
Erythrocyte Osmotic Fragility ◽  
Group I ◽  
Ameliorative Effect ◽  
Soya Oil ◽  
Erythrocyte Fragility

Chronic exposure to chlorpyrifos (CPF) has been shown to cause increased lipoperoxidative changes in the erythrocyte membranes. The relationship between chronic CPF-induced lipoperoxidative changes and erythrocyte fragility has not been elucidated. The aim of the present study is to evaluate the role of lipoperoxidation on CPF-induced erythrocyte fragility and the ameliorative effect of vitamin C. Twenty animals divided at random into four groups of five animals each served as subject for this study. Rats in group I served as the control group and were given only soya oil at a dose of 2 mL/kg body weight (b.w.). Rats in group II were dosed with vitamin C (100 mg/kg b.w.) and then supplemented with soya oil (2 mL/kg b.w.), while those in group III were administered with CPF only at a dose of 10.6 mg/kg b.w. (∼one-eighth of the previously determined median lethal dose [LD50]). Rats in group IV were pretreated with 100 mg/kg b.w. of vitamin C, and then dosed with CPF at a dose of 10.6 mg/kg b.w., 30 min later. The different treatment regimens were orally administered daily for a period of 17 weeks. Blood collected from the animals at the end of the test period were analyzed for erythrocyte osmotic fragility and malonaldehyde (MDA) concentration as an index of lipid peroxidation. The study showed that CPF caused significant increase in erythrocyte fragility and MDA concentration, which were ameliorated by pretreatment with vitamin C. In conclusion, the study showed that CPF-evoked erythrocyte fragility due to increased lipoperoxidative changes was ameliorated by pretreatment with vitamin C.

scholarly journals Ameliorative effect of Alpha-tocopherol on E-cigarette liquid induced histomorphological changes in adrenal cortex of male Albino rats.

2021 ◽  
Vol 28 (04) ◽  
pp. 520-526
Author(s):  
Ali Ahmed ◽  
Mehreen Lashari ◽  
Muhammad Imran Bajwa ◽  
Jahanzaib Lashari ◽  
Syeda Sara Bano ◽  
...  
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Adrenal Cortex ◽  
Alpha Tocopherol ◽  
Control Group ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Ameliorative Effect ◽  
Group Ii

Objective: To study the ameliorative effect of Alpha-Tocopherol (Vitamin E) against E-cigarette induced histomorphalogical changes in adrenal cortex of male Albino rats. Study Design: Randomized Animal Control Trial. Setting: Department of Anatomy, Islamic International Medical College Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad. Period: September 2018 to September 2019. Material & Methods: 30 adult male albino rats were divided in three groups having 10 rats each. Normal diet and distilled water was given to control group (Group I) including 10 rats, for 4 weeks. The Rats of Group II were injected E-cigarette liquid 0.5mg per kg body weight per day diluted in 500ul normal saline intraperitoneally for 4 weeks. The experimental rats in Group III, in addition to E-cigarette liquid also received Vitamin E, 100mg per kg body weight per day mixed in daily diet. Dissection of the animals was done after completion of 4 weeks and adrenal gland was removed for histological analysis. Data was recorded and analyzed in SPSS™ version 21. Results: Alpha-Tocopherol restored the quantitative changes induced by E-Cigarette liquid in adrenal cortex in Group III, which was increased in the rats of Group II who were injected E-Cigarette liquid. Among microscopic parameters Orientation of cells, sinusoidal dilatation was significantly reduced in all adrenal cortex zones by Alpha-Tocopherol in Group III animals. Conclusion: The current study demonstrated that Alpha-Tocopherol had an ameliorative effect on histological changes caused by E-cigarette liquid on adrenal cortex.

Evaluation of Anti Hyperuricemic Activity of Vitamin E on Potassim Oxonate Induced Hyperuricemic Rat Model

2020 ◽  
Author(s):  
Hariprasad Sapkota ◽  
Hemraj Sharma ◽  
Shakti Shrestha ◽  
Sandhya Shrestha ◽  
sudipa Adhikari ◽  
...  
Keyword(s):  
Uric Acid ◽  
Vitamin E ◽  
Serum Albumin Level ◽  
Albumin Level ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Per Oral ◽  
Potassium Oxonate

Abstract Background Hyperuricemia is often associated with oxidative stress and vitamin E alters uric acid level in hypertensive rats, consequently, vitamin E may have a significant role in non-hypertensive hyperuricemia. Therefore, we aimed to evaluate anti-hyperuricemic activity of vitamin E on potassium oxonate-induced hyperuricemic rats. Methods Eighteen adult Wistar Albino rats of similar weights were equally divided into group I (normal control), group II (disease control) and group III (treatment control). Group I received 0.5ml normal saline per oral while group II and group III received potassium oxonate (250 mg/kg-intraperitoneal) on day 1, 3, 6, 10, 13 and 15. Group III also received vitamin E (200 mg/kg-per oral) for 15 days. Blood samples were collected through retro orbital plexus from all the animals on day 15 to evaluate uric acid, creatinine, total cholesterol, triglycerides, albumin and blood urea nitrogen, as well joint diameter, and kidneys’ weight was also evaluated. Result We observed a statistically significant rise (p<0.05 or p<0.001) in all the parameters and a significant decline in serum albumin level (p<0.001) in group II as compared to group I, but no significant difference (p>0.05) in all the parameters between group I and III. In contrast to this, a significant reduction in all the parameters (p<0.05 or p<0.001) and significant increase in serum albumin level (p<0.001) were observed in group III as compared to group II. Conclusion Our findings suggest that vitamin E has an anti-hyperuricemic activity against hyperuricemia induced rats.

Cytoprotective Effect of Tocotrienol-Rich Fraction and α-Tocopherol Vitamin E Isoforms Against Glutamate-Induced Cell Death in Neuronal Cells

2014 ◽  
Vol 84 (3-4) ◽  
pp. 0140-0151 ◽  
Author(s):  
Thilaga Rati Selvaraju ◽  
Huzwah Khaza’ai ◽  
Sharmili Vidyadaran ◽  
Mohd Sokhini Abd Mutalib ◽  
Vasudevan Ramachandran ◽  
...  
Keyword(s):  
Vitamin E ◽  
Cell Viability ◽  
Neuronal Cells ◽  
Positive Control ◽  
Post Treatment ◽  
Mitochondrial Activity ◽  
Before And After ◽  
Pre Treatment ◽  
Tocotrienol Rich Fraction ◽  
Major Mediator

Glutamate is the major mediator of excitatory signals in the mammalian central nervous system. Extreme amounts of glutamate in the extracellular spaces can lead to numerous neurodegenerative diseases. We aimed to clarify the potential of the following vitamin E isomers, tocotrienol-rich fraction (TRF) and α-tocopherol (α-TCP), as potent neuroprotective agents against glutamate-induced injury in neuronal SK-N-SH cells. Cells were treated before and after glutamate injury (pre- and post-treatment, respectively) with 100 - 300 ng/ml TRF/α-TCP. Exposure to 120 mM glutamate significantly reduced cell viability to 76 % and 79 % in the pre- and post-treatment studies, respectively; however, pre- and post-treatment with TRF/α-TCP attenuated the cytotoxic effect of glutamate. Compared to the positive control (glutamate-injured cells not treated with TRF/α-TCP), pre-treatment with 100, 200, and 300 ng/ml TRF significantly improved cell viability following glutamate injury to 95.2 %, 95.0 %, and 95.6 %, respectively (p < 0.05).The isomers not only conferred neuroprotection by enhancing mitochondrial activity and depleting free radical production, but also increased cell viability and recovery upon glutamate insult. Our results suggest that vitamin E has potent antioxidant potential for protecting against glutamate injury and recovering glutamate-injured neuronal cells. Our findings also indicate that both TRF and α-TCP could play key roles as anti-apoptotic agents with neuroprotective properties.

Features of the cervix uteri in women of reproductive age with endometrial polyps and micropolyps

2016 ◽  
pp. 108-111
Author(s):  
T.F. Tatarchuk ◽  
◽  
D.G. German ◽  
Keyword(s):  
Reproductive Age ◽  
Cervix Uteri ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Endometrial Polyps ◽  
Group Iii ◽  
Aggressive Form ◽  
Group I ◽  
Group Ii ◽  
Control Group Group

The article presents the comparative analysis of the state of the cervix in women with endometrial polyps and micropolyps. Patients and methods. The study involved 130 patients aged 18-35 years: 70 patients with endometrial polyps (group I), 30 patients with micropolyps (group II) and 30 patients of the control group (group III). Results. According to the anamnesis of women in the I group were significantly more frequent diseases of the cervix, which corrected physical surgery methods, in particular cryodestruction. In group II, the representatives of these indicators were similar to healthy. Normal colposcopic picture met significantly less frequently in patients and I, and II group. The differences in the incidence of HPV high oncogenic risk in all groups were not statistically significant. Conclusion. Destructive methods used in the detection of any changes in the cervix are often overly aggressive, form scars and contributing to inflamaciones process. In the chain of events leading to the formation of PE, cervical pathology and its correction can take the basic place. Key words: endometrial polyp, micropolyps, chronic endometritis, uterine cervix, colposcopy.

scholarly journals Clinical outcomes of submassive pulmonary embolism thrombolysis—an Indian experience

2020 ◽  
Vol 72 (1) ◽  
Author(s):  
Nadeem U. Rehman ◽  
Mohd Iqbal Dar ◽  
Manish Bansal ◽  
R. R. Kasliwal
Keyword(s):  
Pulmonary Embolism ◽  
Pulmonary Artery ◽  
Systolic Dysfunction ◽  
Massive Pulmonary Embolism ◽  
Post Treatment ◽  
Control Group ◽  
Submassive Pulmonary Embolism ◽  
Venous Thromboembolic ◽  
Pre Treatment ◽  
And Control

Abstract Background Acute pulmonary thromboembolism is the most dangerous presentation of venous thromboembolic disease. The role of thrombolysis in massive pulmonary embolism has been studied extensively, but the same is not there for submassive pulmonary embolism. This study is aimed at evaluating the effects of thrombolysis in acute submassive pulmonary embolism. This was a prospective, case-control, observational study. Patients presenting with acute submassive pulmonary embolism were divided into thrombolysis group and control group depending on whether they received thrombolysis plus anticoagulation or anticoagulation only, respectively. Results A total of 86 patients were included in the study. Forty-two patients were in the thrombolysis group, and 44 patients were in the control group. The mean ± SD age in the control and thrombolysis groups was 63.3 ± 14.7 and 56.4 ± 13.8 years, respectively. The two groups were well matched in sex distribution and associated comorbidities like COPD, active surgery, major trauma, and immobilization. On echocardiography, dilated RA/RV in pre-treatment vs. post-treatment was seen in 20 (45.5%) vs. 20 (45.5%) in the control group and 26 (61.9%) vs. 11 (26.2%) in the thrombolysis group. Similarly, RV systolic dysfunction in pre-treatment vs. post-treatment was seen in 24 (54.5%) vs. 21 (47.7%) in the control group and 22 (52.4%) vs. 8 (19.0%) in the thrombolysis group. Pulmonary artery pressure in pre-treatment vs. post-treatment was 64.4 ± 15.0 vs. 45.9 ± 9.9 mmHg in the control group and 68.3 ± 17.4 vs. 31.4 ± 6.9 mmHg in the thrombolysis group. In control vs. thrombolysis group, there were 5 vs. 1 death, 6 vs. 1 hemodynamic decompensation, and 6 vs. 1 patient needing mechanical ventilation. Conclusion Thrombolysis in submassive pulmonary embolism is associated with better right ventricular functions, lower pulmonary artery pressures, and comparable mortality rates.

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