scholarly journals Ameliorative effect of Alpha-tocopherol on E-cigarette liquid induced histomorphological changes in adrenal cortex of male Albino rats.

2021 ◽  
Vol 28 (04) ◽  
pp. 520-526
Author(s):  
Ali Ahmed ◽  
Mehreen Lashari ◽  
Muhammad Imran Bajwa ◽  
Jahanzaib Lashari ◽  
Syeda Sara Bano ◽  
...  
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Adrenal Cortex ◽  
Alpha Tocopherol ◽  
Control Group ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Ameliorative Effect ◽  
Group Ii

Objective: To study the ameliorative effect of Alpha-Tocopherol (Vitamin E) against E-cigarette induced histomorphalogical changes in adrenal cortex of male Albino rats. Study Design: Randomized Animal Control Trial. Setting: Department of Anatomy, Islamic International Medical College Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad. Period: September 2018 to September 2019. Material & Methods: 30 adult male albino rats were divided in three groups having 10 rats each. Normal diet and distilled water was given to control group (Group I) including 10 rats, for 4 weeks. The Rats of Group II were injected E-cigarette liquid 0.5mg per kg body weight per day diluted in 500ul normal saline intraperitoneally for 4 weeks. The experimental rats in Group III, in addition to E-cigarette liquid also received Vitamin E, 100mg per kg body weight per day mixed in daily diet. Dissection of the animals was done after completion of 4 weeks and adrenal gland was removed for histological analysis. Data was recorded and analyzed in SPSS™ version 21. Results: Alpha-Tocopherol restored the quantitative changes induced by E-Cigarette liquid in adrenal cortex in Group III, which was increased in the rats of Group II who were injected E-Cigarette liquid. Among microscopic parameters Orientation of cells, sinusoidal dilatation was significantly reduced in all adrenal cortex zones by Alpha-Tocopherol in Group III animals. Conclusion: The current study demonstrated that Alpha-Tocopherol had an ameliorative effect on histological changes caused by E-cigarette liquid on adrenal cortex.

scholarly journals Differential Expression of CD3, TNF-α, and VEGF Induced by Olanzapine on the Spleen of Adult Male Albino Rats and the Possible Protective Role of Vitamin C

Biomedicines ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 39
Author(s):  
Sahar Youssef ◽  
Marwa Salah
Keyword(s):  
Body Weight ◽  
Vitamin C ◽  
Adult Male ◽  
Control Group ◽  
Albino Rats ◽  
White Pulp ◽  
Group Iii ◽  
Group I ◽  
Tnf Α ◽  
Group Ii

Olanzapine is an antipsychotic drug effective in the treatment of stress-associated psychiatric illnesses, but its effect on the spleen remains unclear. Vitamin C is essential for the optimum function of the immune system. We aim to investigate the effect of Olanzapine on spleen structures and to assess the protective effect of vitamin C. Forty adult male albino rats were divided into four groups: group (I), a control; group (II), rats were given vitamin C at 40 mg/kg body weight; group (III), rats were given Olanzapine at 2 mg/kg body weight; and group (IV), rats were given vitamin C and Olanzapine at the same dose of group (II) and group (III) for one month. The hematoxylin and eosin (H&E) of the olanzapine treated group showed focal areas of cellular depletion and a decrease in the size of the white pulp. The red pulp was expanded and showed marked congestion and dilatation of blood sinusoids. Cluster of differentiation 3 (CD3) was significantly reduced, however both tumor necrosis factor alpha (TNF-α), and vascular endothelial growth factor (VEGF) were significantly higher. The administration of vitamin C repaired structural and immunohistochemical changes via increased CD3 and decreased TNF-α and VEGF. Therefore, the oxidative and the inflammatory pathways may be the possible mechanisms underlying olanzapine immunotoxicity. Vitamin C exerted immune modulator and antioxidant effects against olanzapine.

Effect of vitamin E on reproductive function in the mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin*

2008 ◽  
Vol 24 (9) ◽  
pp. 595-601 ◽  
Author(s):  
JP Xu ◽  
YP Yin ◽  
XQ Zhou
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Survival Rate ◽  
Sex Ratio ◽  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Experimental Group

The study was conducted to determine the effects of vitamin E on reproductive performance in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The 75 female and 40 male mice were randomly assigned into five groups. The levels of both TCDD and vitamin E given by gavage were 0 and 0 (Control group), 100 and 0 (experimental group I), 100 and 20 (experimental group II), 100 and 100 (experimental group III), and 100 ng/kg/day and 500 mg/kg/day (experimental group IV), respectively. Males and females were mixed to mate at the ratio of 1:2 after 4-week experiment. The gavage treatments were continued until the end of gestation in female mice after mating. The results showed that the litter number, survival rate, and body weight at birth of offspring in experimental group I had significantly decreased, and the females’ pregnancy rate and pup sex ratio in experimental group I had the decreasing tendencies when compared with the control group. The litter number in experimental group III, survival rate in experimental group II and III, body weight at birth in experimental group III and IV exhibited significant increase compared with experimental group I. The female pregnancy rate in both experimental group III and IV recovered to 100%, but there were no significant differences when compared with experimental group I. The pups’ sex ratio had a gradually increasing tendency with increase of vitamin E level, but there was no significant difference among experimental group I–IV. The results suggest that TCDD could induce reproductive toxicity in mice, whereas vitamin E alleviated adverse effects on reproductive performance in mice caused by TCDD.

scholarly journals Effect of Sofosbuvir Administration and Its Withdrawal on the Submandibular Salivary Gland of Adult Male Albino Rats: A Histological and Ultra-Structural Study

2019 ◽  
Vol 7 (23) ◽  
pp. 4101-4109
Author(s):  
Asmaa Mahmoud Abdeen ◽  
Tarik Essawy ◽  
Saher Sayed Mohammed
Keyword(s):  
Salivary Gland ◽  
Inflammatory Cells ◽  
Control Group ◽  
Albino Rats ◽  
Submandibular Salivary Gland ◽  
Distilled Water ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Histological Results

BACKGROUND: Sofosbuvir (SOF) was published in 2013 as a part of first-line treatments for hepatitis C virus (HCV); it has activity against all genotypes with extrahepatic adverse effects have recently arisen. AIM: Investigating sofosbuvir-induced alterations in the rat submandibular salivary gland (SMSG). METHODS: A group of 80 adult albino rats weighing about ± 150 gm were used in the experiment. The rats were divided into 3 groups: Group I (control group) received distilled water, Group II (experimental group) divided into 2 subgroups and received SOF 40 mg/kg/day dissolved in distilled water for 1 and 3 months and Group III (recovery group) allowed for 1 month of recovery after SOF withdrawal. All animals were sacrificed; the SMSG was dissected, and specimens were examined histologically and ultra-structurally. RESULTS: Compared to Group I, Group II subgroup (1) showed acinar and ductal vacuolisation, discontinuity of the epithelial lining associated with retained secretion and congested blood vessels. These changes were found to be exaggerated in the subgroup (2) accompanied by acinar and ductal shrinkage, interstitial oedema, haemorrhage, chronic inflammatory cells infiltration and loss of gland compactness. Amelioration of the histological changes was detected in Group III after SOF withdrawal. The ultrastructural examination confirmed the histological results. CONCLUSION: SOF had induced apparent alterations in the structure and ultrastructure of SMSG. The SOF-induced alterations were time-dependent, attributed mainly to mitochondrial toxicity and partially ameliorated by its withdrawal.  

scholarly journals The effect of magnetic field on hatchability of Japanese quail eggs

2011 ◽  
Vol 51 (No. 8) ◽  
pp. 355-360 ◽  
Author(s):  
Z. Tarasewicz ◽  
D. Szczerbinska ◽  
D. Majewska ◽  
A. Danczak ◽  
M. Ligocki ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Body Weight ◽  
Variable Magnetic Field ◽  
Control Group ◽  
Average Weight ◽  
Group Iii ◽  
Egg Hatchability ◽  
Group I ◽  
Group Ii ◽  
Similar Body

The effect of environmental conditions on hatching indices of poultry eggs is sufficiently important that new methods for their improvement are sought, among others through exposing the eggs during hatching to an artificially generated magnetic field of variable frequency. Hatching eggs in this study came from Pharaoh quail in the 4th month of laying. The flock was kept under optimum microclimate conditions and fed a complete feed mix containing 21% total protein and 11.7 MJ ME. The eggs (n = 150), after weighing, were divided into 3 groups equal in respect of numbers: control (I) and two experimental (II and III). The eggs of group II and III were exposed to the action of variable magnetic field of the same intensity but different times of application. The highest percentage of dead embryos during incubation in relation to fertilised eggs was found in control group (11.36%), while the smallest was from group II (4.17%). The highest value of hatching indices calculated in relation to fertilised eggs was found in group II (91.6%), while the smallest was in group III (83.7%) with 86.3% in control group. The results point to the possibility of increasing egg hatchability indices through the use of additional variable magnetic field. The chicks hatched from eggs exposed to the action of this experimental agent had similar body weight. The average weight of one-day-old chicks ranged from 7.82 g (group II) to 8.05 g (group III). In the last week of rearing, mean body weight in both sexes was similar and ranged from 168 (group I) to 172 g (group III) in males, and from 186 g (group I) to 199 g (group III) in females; these differences were not statistically significant. The females of group III reached sexual maturity at 41 days, this being one and three days (non-significantly) earlier than birds in group I and II.  

scholarly journals Effect of Amomum subulatum seeds against cypermethrin induced haematological changes in wistar albino rats

2019 ◽  
Vol 8 (3) ◽  
pp. 604
Author(s):  
Goutham Sagarkatte Puttanna ◽  
Purushotham K. ◽  
Swarnalatha Nayak ◽  
Eesha B. Rao ◽  
Ravi Mundugaru
Keyword(s):  
Body Weight ◽  
Normal Control ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Test Drug ◽  
Group Iii ◽  
Group I ◽  
Amomum Subulatum ◽  
Wistar Albino Rats

Background: Cypermethrin is a well know agricultural pesticide used in the developing countries. It is associated with significant toxic potential on human health. Hence the present study was aimed to evaluate the protective role of Amomum subulatum against cypermethrin induced haematalogical changes in Wistar albino rats.Methods: The albino rats were divided into five different groups of six rats each. Group I considered as normal control, group II cypermethrin control (25mg/kg body weight p.o.), group III only test drug and group IV and V administered with cypermethrin 25mg/kg body weight along test drug 1.08 and 2.16mg/kg body weight for 28 consecutive days. At the end of 28th day blood was withdrawn and total haematalogical parameters were estimated.Results: In the cypermethrin control there was significant reduction in the WBC, Platelet, MCHC and considerable reduction in the haemoglobulin concentration in comparison to normal control. The test drug administered at both dose levels was significantly reversed the cypermethrin induced changes in haematalogical parameters.Conclusions: Authors can conclude that the Amomum subulatum has potency to reverse the cypermethrin induced haematalogical changes.

Ameliorative effect of curcumin against tamoxifen in-duced hepatotoxicity in female albino rats

2017 ◽  
Vol 2 (2) ◽  
pp. 223-239
Author(s):  
Saber A. Sakr ◽  
Hoda A. Mahran ◽  
Hagar A. Abu Quora
Keyword(s):  
Body Weight ◽  
Dose Level ◽  
Biochemical Markers ◽  
Antioxidant Activities ◽  
Control Group ◽  
Albino Rats ◽  
Bile Duct Proliferation ◽  
Leukocytic Infiltration ◽  
Group I ◽  
Ameliorative Effect

The objective of the present work was to investigate the ameliorative effect of curcumin (Cur) against tamoxifen (TAM), a nonsteriodal antiestrogenic drug, induced hepatotoxicity in adult female albino rats. Forty eight female albino rats were divided into four groups of equal size. groups: group (I) animals of this group were served as control, group (II) rats were orally given Cur at a dose level of 150 mg/kg body weight daily for nine weeks, group (III) rats were orally given TAM at a dose level of 20 mg/kg body weight daily for nine weeks and group (IV) rats were given TAM followed by Cur at the same previous doses daily for nine weeks. For histological and immunohistochemical studies, livers were immediately removed after sacrification and sera were collected to detect the biochemical markers. Liver sections of rats treated with TAM showed loss of the normal architecture, cytoplasmic vacuolation, leukocytic infiltration and bile duct proliferation as well as increase in the expression of both alpha smooth muscle actin (α-SMA) and Ki-67. The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the level of malondialdehyde (MDA) were elevated, while the activities of catalase (CAT), superoxide dismutase (SOD) and the level of glutathione (GSH) were decreased in sera of rats of TAM group. On the other hand, liver sections of rats treated with TAM followed by Cur showed improvement in the histological, immunohistochemical and the biochemical markers. In conclusion, Cur revealed ameliorative effect against TAM toxicity in rat liver due to its antioxidant activities.

scholarly journals Supplementation effect of medicinal plant leaves on growth in crossbred calves

2020 ◽  
Vol 34 (03) ◽  
Author(s):  
Ritu Raj ◽  
Anshu Rahal ◽  
Anil Kumar ◽  
S. K. Singh
Keyword(s):  
Body Weight ◽  
Dry Matter ◽  
Group Iv ◽  
Dry Matter Intake ◽  
Tinospora Cordifolia ◽  
Control Group ◽  
Mentha Arvensis ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Effect of supplementing giloy (Tinospora cordifolia) and mint (Mentha arvensis) on feed intake and body weight was studied in twenty crossbred calves. While Group I was taken as control, Group II was supplemented with Tinospora cordifolia @ 4% of concentrate, group III with Mentha arvensis @ 4% of concentrate and group IV with both Tinospora cordifolia and Mentha arvensis @ 2% of concentrate each. Significantly higher(P 0.05) dry matter intake(Kg) and dry matter intake(g)/W 0.75Kg in group III was recorded compared to control while group II did not differ from group IV. Significantly higher body weight gain was noticed in group IV compared to other groups. Giloy and/or mint can be fed to improve growth in crossbred calves.

scholarly journals Ameliorating Effect of Combined Cinnamon and Ginger Oils against the Neurotoxicity of Nicotine Administration on the Prefrontal Cortex of Adult Albino Rats: Immunohistochemical and Ultrastructural Study

2021 ◽  
Vol 89 (3) ◽  
pp. 41
Author(s):  
Medhat Taha ◽  
Mohie Mahmoud Ibrahim ◽  
Mamdouh Eldesoqui ◽  
Mohamed A. M. Iesa ◽  
Tourki A. S. Baokbah ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Prefrontal Cortex ◽  
Group Iv ◽  
Tissue Homogenate ◽  
Control Group ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Tnf Α ◽  
Group Ii

Background: Nicotine is the active alkaloid in cigarettes. It was reported that tobacco smoking has many hazards; one of these hazards is the effect on the cognitive function of the prefrontal cortex. The aim of our study is to investigate the antioxidant effects of ginger, cinnamon oils, and their combination on morphological changes in the prefrontal cortex that were induced by nicotine. Materials and methods: Fifty adult male albino rats were divided into five groups: group I (control group), group II (nicotine), group III (nicotine + cinnamon), group IV (nicotine + ginger), and group V (nicotine + cinnamon + ginger). The coronal sections from the anterior part of the rat brain at the site of prefrontal cortex were examined by light microscope for (H&E and immunohistochemical staining with TNF-α and GFAP), while the ultrastructure morphology was examined by transmission electron microscopy. Levels of the oxidative stress markers (MDA, GSH) in the rats’ brain tissue homogenate were biochemically assessed. Results: Compared to the control group, the rats that were treated with nicotine (group II) showed a significant oxidative stress in the form of marked elevation of MDA and decrease in GSH, apoptotic changes especially in the pyramidal cells in the form of neuronal cell degeneration and pyknosis, and an elevation in the inflammatory marker TNF-α and GFAP expressions. These changes were observed to a lesser degree in rat group (III) and group (IV), while there was a marked improvement achieved by the combined usage of cinnamon and ginger oils, together compared to the nicotine group. Conclusions: Ginger and cinnamon are powerful antioxidants which ameliorate the degenerative and oxidative effects produced by nicotine on a rat’s prefrontal cortex.

scholarly journals Sargassum crasifolium Extract Could Prevent the Decrese in the Thyroxine Hormone and the Body Weight Loss of Javanese Randu Goats during Transportation

2020 ◽  
pp. 335-343
Author(s):  
Claude Mona Airin ◽  
Amelia Hana ◽  
Sarmin ◽  
Pudji Astuti ◽  
Amir Husni ◽  
...  
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Vitamin B1 ◽  
Body Weight Loss ◽  
The Body ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Before And After ◽  
Group Ii

Thyroxine hormone (T4) was one of the hormones related to metabolism rate. Animal transportation could be stressor interfering metabolism. Sargassum crasifoium was one of brown algae species that contained some important nutrients and also flavonoids. The study aimed at finding out the effect of the Sargassum on the decrase in thyroxine hormone and body weight loss. It used 9 Javanese Randu goats that were classified into 3 treatment groups. Group I served as control, Group II was treated with 450 mg/kg BW of Sargassum estract and Group III was treated with vitamin B1. All of the goats were transported for 12 hours using pickup truck at the speed of 60 km/hour. Blood samples were drawn before the transportation, during loading, 6 hours after the transportation and at the end of the transportation, while the body weight of the goats was weighed before and after the transportation. The results of the study showed that the T4 hormone decreased in the Group I and increased in the Group III during the transportation, while it was stable in the Group II. The biggest percentage of the body weight loss took place to the Group III (0.13%), while the smallest percentage took place to the Group II (0.063%). Statistical analysis showed that the Sargassum extract did not have any significant effect (p>0.05) on both the thyroxine hormone and the body weight loss during the transportation. Based on the results of the study it could be concluded that the Sargassum extract could stabilize the thyroxine hormone during the transportation so that it could prevent the body weight loss

Light Microscopic Study on Possible Protective Effect of Vitamin E on Imatinib IHCV Dilatation in Albino Rats

2021 ◽  
Vol 15 (12) ◽  
pp. 3185-3187
Author(s):  
Nighat Ara ◽  
Zahid Sarfaraz ◽  
Rifat Shamim ◽  
Zafar Iqbal ◽  
Farooq Khan ◽  
...  
Keyword(s):  
Vitamin E ◽  
Protective Effect ◽  
Hepatic Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Sprague Dawley ◽  
Group Iii ◽  
Medical College ◽  
Group Ii

Aim: To observe the changes in the caliber of hepatic central vein of Albino rats exposed to the oral administration of toxic doses of Imatinib and to assess the protective effect of vitamin E. Study design: Experimental study Place and duration of study: Department of Pharmacy, Peshawar Medical College Peshawar from 1st October 2015 to 31st March 2016. Methodology: Twenty four male Sprague-Dawley rats (150- 200g) were selected for this study and these rats were divided into three groups. Group 1 was control group, Group II received oral Imatinib solution (50 mg/kg/day) and Group III received Imatinib solution (50mg/kg/day) orally along with vitamin- E (500MG/kg/day) for 14 days. At the end of experiment central vein dilatation in liver tissue of rats were observed. Results: The increase in mean central vein dilatation in liver of group II were statistically significant (p=0.001) in comparison with the control. The mean central vein caliber of liver was decreased significantly in group III. Conclusion: The simultaneous use of vitamin E as an antioxidant with Imatinib can protect the toxic effects of the Imatinib on hepatic toxicity which includes central vein dilatation. Keywords: Imatinib mesylate, Hepatic tissue, Central vein dilatation, Vitamin E

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