EXTRACTION OF FLAVONOIDS FROM THE LEAVES OF THE LITTLEWALE LITHOSPERMUM OFFICINALE L. (BORAGINACEAE) USING MICROWAVE ENERGY

One of the possible ways to intensify and increase the efficiency of extraction of biologically active substances from plant materials is the use of microwave radiation. This article presents the results of a study of the influence of parameters of the microwave extraction process on the output of flavonoids from the leaves of the littlewale (Lithospermum officinale L.), belonging to the Boraginaceae family. This plant contains isoquercitrin, which is one of the key flavonoids with the property of tissue regeneration. It was shown that the maximum yield of the target components is achieved with a microwave generator power of 100 W and exposure duration of 2.5 min at a field frequency of 2450 MHz (specific energy consumption is 8.4 kWh/kg). This output is comparable with the output when using conventional convection heating. Microwave radiation provides a 16-fold reduction in the duration of extraction of the sum of flavonoids and an increase in the degree of extraction of isoquercitrin by 23%. The action of microwave energy leads to the destruction of plant tissue, which contributes to a more efficient extraction of flavonoids from plant raw materials.

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PRODUCTION OF EXTRACTS FROM VEGETABLE RAW MATERIALS BY CARBON DIOXIDE

Food Science and Technology ◽

10.15673/fst.v14i1.1651 ◽

2020 ◽

Vol 14 (1) ◽

Author(s):

K. Gafurov ◽

B. Muhammadiev ◽

Sh. Mirzaeva ◽

F. Kuldosheva

Keyword(s):

Carbon Dioxide ◽

Supercritical Co2 ◽

Raw Materials ◽

Maximum Yield ◽

Volumetric Flow Rate ◽

Extraction Process ◽

Raw Material ◽

Critical Conditions ◽

Plant Materials ◽

Laboratory Setup

The unique properties of supercritical carbon dioxide as a solvent are widely used for extraction. In supercritical media, the dissolution of molecules of various chemical nature is possible. The purpose of this investigation was to study the extraction process and obtain extracts from valuable regional plant materials by applying CO2 extraction under pre- and supercritical conditions. The objects of research were: ground seeds of melon, pumpkin and licorice roots, as well as mint leaves, mulberry and jida flowers. For extraction, a laboratory setup was used that allows extraction when the CO2 is supplied by a high-pressure plunger pump in the sub- and supercritical state using a heat pump. The pressure range is 3-15 MPa, temperatures 295–330 K, and the volumetric flow rate above the critical CO2 is 800–900 g. Experiments with ground seeds of melon and pumpkin showed that as a result of 4 sequentially performed extraction cycles on a single load with supercritical CO2 parameters ( 315–330 K; 3–7.5 MPa) the decrease in the mass of melon seeds was 90 g (pumpkins 80 g). During the total extraction time (2.5 hours), 20 kg of CO2 were pumped through the reactor (25 l at 290 K and 6.8 MPa), while the average oil content in the extract was 4 g per 1 kg of CO2 (3.0 g per 1 l of SС-CO2) In experiments with jida flowers, the maximum amount of solid extractable substance (2% by weight of the raw material) was obtained at a temperature in the extractor of 308 K and a pressure of 7.5 MPa. Upon extraction under critical conditions in collection 2, the liquid phase was absent; only a yellow-green paste was released in it. According to the results of experiments with mint leaves, the maximum yield of a greenish liquid was observed at T = 315 K and P = 4 MPa., Mulberry - at T = 306 K and P = 6.0 MPa. The results of the extraction of oils and extracts from ground seeds of melon, pumpkin and licorice roots, as well as mint leaves, mulberries and jida flowers confirm that the maximum yield of the extracted substance is achieved with supercritical CO2 parameters in the extractor (310 K, 7.5 MPa). When liquid CO2 is extracted (300 K and 6-8 MPa), up to 2% of a yellow substance is extracted, which does not differ in appearance from a supercritical extract.

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Modern research on the extraction of biologically active substances from plant raw materials using enzymes

New Technologies ◽

10.47370/2072-0920-2021-17-2-56-66 ◽

2021 ◽

Vol 17 (2) ◽

pp. 56-66

Author(s):

A. A. Fabritskaya ◽

S. O. Semenikhin ◽

V. O. Gorodetsky ◽

N. I. Kotlyarevskaya ◽

E. P. Victorova

Keyword(s):

Ultrasonic Treatment ◽

Raw Materials ◽

Microwave Treatment ◽

Extraction Process ◽

Biologically Active Substances ◽

Biologically Active ◽

Plant Materials ◽

Plant Raw Materials ◽

Treat Ment ◽

Active Substances

The article overviews modern studies of domestic and foreign scientists in the field of extraction of biologically active substances from plant materials using enzymes. Extraction of biologically active substances using enzymes allows for controlled biotransformation of plant materials due to the precise specificity and selectivity of enzymes, which creates mild conditions that ensure the preservation of biologically active substances. It has been noted that the quality of the extractant used has a significant effect on the efficiency of the extraction process. Thus, raw materials treated with a combination of an electrolyte and an enzyme are extracted more efficiently by reducing the mass transfer barrier. The main advantage of using supercritical fluids as an extractant is a decrease in temperature or pressure that leads to the precipitation of the extractable substance. One of the main disadvantages of the process of extracting biologically active substances from plant materials using enzymes is their high cost. To eliminate this disadvantage, the following intensification methods are used: ultrasonic treatment, high pressure treatment and microwave treat- ment. The most promising methods for intensifying the process of extracting biologically active substances from plant materials using enzymes are methods of ultrasonic and microwave treatment. Ultrasonic treatment under optimal conditions allows increasing the activity of enzymes, and microwave treatment provides more efficient penetration of the extractant into the tissues of plant material, due to the destruction of cell walls.

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FRUIT SEEDS, SEEDS OF GRAPE AND CUCURBITACEOUS – A PROSPECTIVE SOURCE OF OBTAINING BIOLOGICALLY ACTIVE SUBSTANCES. OVERVIEW

Food Science and Technology ◽

10.15673/fst.v15i1.1964 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

K. Gafurov ◽

B. Muslimov

Keyword(s):

Vegetable Oil ◽

Raw Materials ◽

Maximum Yield ◽

Engineering Calculation ◽

Biologically Active Substances ◽

Biologically Active ◽

High Quality ◽

Plant Materials ◽

Cold Pressing ◽

Active Substances

Republic of Uzbekistan is a major producer of agricultural products. The production of vegetables, grapes, fruits and berries, melons and gourds are increasing every year. The total volume of their production is approximately up to 16200 thousand tons per year. The seeds of these crops make up from 2 to 10% of the mass of processed raw materials, which are sources of valuable biologically active substances. The main method of obtaining oil (from apricot kernel, grape and melon seeds) is cold pressing, i.e. pressing carried out at a temperature below 60°C. Such oil immediately goes from the category of ordinary to the category of medicinal. At present, in Uzbekistan, the processing of apricot kernels, grape and melons seeds with the aim of to obtain high-quality vegetable oil is carried out on low-tonnage technological lines by the method of cold pressing. However, this method of processing raw materials does not provide the maximum yield of valuable vegetable oil and nutrients. Residual oilcake is not processed to obtain biologically active substances. The use of extraction as a method of obtaining these substances is not practiced. Unlike other solvents such as butane, hexane has the advantage of being virtually harmless to the user in ethanol extraction. Unlike the so-called "CO2-extraction", the extraction with ethanol can be carried out without the use of expensive equipment and subject to the correct technological regulations; the obtained extract can be of a very high-quality spectrum. A scientifically substantiated methodology for the engineering calculation of a plant for the extraction of ingredients from plant materials with a low capacity, operating on an ethanol solvent, has not been developed, which shows the relevance of the development and implementation of such equipment in production.

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Fast screening of some flavonoids content in raw plant materials: opportunities of 1H NMR spectroscopy

E3S Web of Conferences ◽

10.1051/e3sconf/202016902006 ◽

2020 ◽

Vol 169 ◽

pp. 02006

Author(s):

Gennady Kalabin ◽

Vasilii Vasil’ev ◽

Vasilii Ivlev ◽

Vasilii Babkin

Keyword(s):

Nmr Spectroscopy ◽

Plant Biomass ◽

Raw Materials ◽

Biologically Active ◽

Raw Material ◽

Standard Samples ◽

Plant Materials ◽

H Nmr ◽

Fast Screening ◽

Raw Plant Materials

Environmental monitoring and assessment of the prospects for extracting biologically active substances (BAS) from various types of plant biomass requires the development of simple and fast methods for measuring their content in raw materials. A new approach for measuring the content of various flavonoids groups in plant raw material using 1H NMR spectroscopy has been developed, which allows to characterize its resource capabilities and study the effects on their composition different environmental factors without complex sample preparation and standard samples.

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Prospects for the use of protein-carbohydrate complex based on mung bean seeds in the functional meat products technology

E3S Web of Conferences ◽

10.1051/e3sconf/202017508004 ◽

2020 ◽

Vol 175 ◽

pp. 08004 ◽

Cited By ~ 1

Author(s):

Andrey Alexeev ◽

Tatyana Alexeeva ◽

Larisa Enaleva ◽

Tatyana Tupolskikh ◽

Nataliia Shumskaia

Keyword(s):

Mung Bean ◽

Raw Materials ◽

Meat Products ◽

First Grade ◽

Biologically Active ◽

Plant Materials ◽

Biological Value ◽

Functional Additives ◽

Carbohydrate Complex ◽

Germinated Seeds

The use of new non-traditional types of plant materials in the diet is one of the ways to improve the meat products’ quality. The combination of plant proteins with animals creates biologically active amino-acid complexes which increase its bioavailability for organism and biological value of the product. In this case, the use of non-traditional plant materials in the production technology of new types of sausage products to improve the quality of the end product is prospective and relevant. Research objective is development of formulations and technologies for new types of high biological value products, based on combination of meat raw materials with functional additives of plant origin. The work was performed at the Department of Food Technology FSBEI HE «Don State Agrarian University», Persianovsky, Rostov region. The objects of research were the new protein-carbohydrate complexes based on flour from germinated seeds of mung bean, control and model forcemeat based on trim beef of first grade, lean trim pork and mid fat trim pork, and end products. Introduction to the cooked sausage products formulation of mung bean germinated seeds flour has a beneficial effect on the organoleptic properties of the end product and enriches it with biologically valuable substances.

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Formula and Technology Development for Obtaining Biologically Active Natural Food Additives

Food Processing Techniques and Technology ◽

10.21603/2074-9414-2018-3-105-114 ◽

2019 ◽

Vol 48 (3) ◽

pp. 105-114 ◽

Cited By ~ 1

Author(s):

Станислав Смирнов ◽

Stanislav Smirnov ◽

Олия Фазуллина ◽

Oliya Fazullina

Keyword(s):

Dietary Supplements ◽

Raw Materials ◽

Import Substitution ◽

Food Additives ◽

Experimental Studies ◽

Scientific Data ◽

Biologically Active ◽

Natural Food ◽

Plant Materials ◽

Natural Components

The research featured the development of formulae and technologies for the production of biologically active additives (BAA) for functional foods with potential adaptogenic, antioxidant, and hepatoprotective properties. Shilajit, a natural mineral-organic substrate, and various plant materials were used as sources of natural biologically active substances (BAS). The relevance of the study comes from the need for more efficient use of natural biologically active compounds, as well as from the need to expand the range of domestic dietary supplements with natural ingredients, which resulted from the current import substitution. Scientific data and experimental studies made it possible to choose advantageous raw materials with BAS and to assess their safety and potential properties. An experiment determined the content of BAS in the raw materials and in the control samples. A compatibility test wasconducted by mixing. As a result, five formulae with various combinations of natural components in the composition were scientifically substantiated. The authors defined the physicochemical and technological properties of the mixtures of dry extracts that determined the choice of technology and the quality of the finished product. The present paper also describes technology of obtaining encapsulated dietary supplements based on dry extracts of medicinal plants and shilajit. The advantage of the technology lies in the use of natural components and special processing methods of BAS preservation. The research included the method of water-activated granulation while 20% ethyl was used as a wetting agent. To obtain the granulate, lactose (Russia) and AEROSIL® 200 Pharma (Evonik Industries, Germany) were used as additive agents. As a result, capsules with a dosage of 700 mg were developed. The BAA can be produced on food and pharmaceutical plants.

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Kinetics of Extraction of Dioscine from Vegetable Raw Materials in Vibrocavitation Homogenizer

Drug development & registration ◽

10.33380/2305-2066-2020-9-2-77-81 ◽

2020 ◽

Vol 9 (2) ◽

pp. 77-81 ◽

Cited By ~ 1

Author(s):

E. V. Flisyuk ◽

S. S. Belokurov ◽

I. A. Narkevich ◽

A. N. Shikov ◽

O. M. Flisyuk ◽

...

Keyword(s):

Mass Transfer ◽

Plant Material ◽

Raw Materials ◽

Extraction Process ◽

Rotor Speed ◽

Plant Materials ◽

Periodic Action ◽

Initial Stage ◽

Working Volume ◽

Kinetics Of

Introduction. In recent years, substances extracted from plant materials have been widely used in the pharmaceutical, cosmetic and food industries. Such substances are used as solutions, dry extracts for the manufacture of medicines, dietary supplements, cosmetic creams, food additives in various forms – tablets, capsules, solutions, granular powders. The extraction of valuable substances from plant materials is carried out using the extraction process, which is carried out by various methods and in apparatuses of various designs. Earlier, a comparative study of the extraction of dioscin from fenugreek by various methods was carried out: in devices with a stirrer, in an ultrasonic field, supercritical, fluid CO2 extraction, and in a vibro-cavitation homogenizer. It is shown that the most effective method is the extraction carried out in a vibrocavitation homogenizer.Aim.To analyze the kinetics of the extraction of dioscin from fenugreek seeds, to determine the optimal values of the required degree of grinding of the raw materials, working temperature, the concentration of ethyl alcohol in the solution and the rotational speed of the rotor of the vibrocavitation homogenizer. Determine the effective mass transfer coefficient responsible for the intensity of mass transfer inside the particles.Materials and methods. An experimental study of the extraction of valuable components from plant materials was carried out in a laboratory unit with a vibrocavitation homogenizer of periodic action. As raw materials were used seeds of hay fenugreek, ecotype of Morocco, acquired in the company Fitokasa, Casablanca (Morocco), which we used for research. Commodity analysis showed that raw materials comply with the requirements of the State Pharmacopoeia XIVth edition. As extractants, aqueous solutions of ethanol with an alcohol concentration of 40, 50, 60, 70, 80, and 90 %. The analysis of the kinetics of the process was based on the following ideas. Extraction begins with the surface of the particles of plant material. As the extracted component moves into the volume of the solution, the extractant penetrates into the internal pores of the particles, and the surface on which the extractant and the extracted component interact is gradually shifted into the individual particles. In this case, the resistance to mass transfer in the region between the specified surface and the outer surface of the particle increases over time.Results and discussion. An analysis of the results shows that the rotor speed significantly intensifies the process. In addition, the influence of the rotor speed is most pronounced at the initial stage of the process, when the surface layers of particles of plant material are extracted. It was also found that the resistance to mass transfer inside particles increases significantly as it approaches the final stage of the process, and with an increase in the rotor speed, it increases, especially at the initial stage of the process, which is associated with the intensity of cavitation and the weakening of its effect as the process deepens inward particles.Conclusions. The obtained dependences are necessary to determine the duration of the extraction process in a batch mode, or the average residence time of seeds in the working volume when organizing the process in a continuous mode.

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Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn

Drug development & registration ◽

10.33380/2305-2066-2020-9-1-40-45 ◽

2020 ◽

Vol 9 (1) ◽

pp. 40-45

Author(s):

O. V. Trineeva ◽

A. A. Gudkova ◽

M. A. Rudaya

Keyword(s):

Medicinal Plant ◽

Raw Materials ◽

Herbal Medicines ◽

Sea Buckthorn ◽

Biologically Active ◽

Plant Materials ◽

Fatty Oil ◽

Luminescent Analysis ◽

Diagnostic Signs ◽

Promising Source

Introduction. Sea buckthorn (Hippophae rhamnoides), the family Eleagnaceae – a promising source of biologically active substances, the traditional raw materials of which are fruits. The existing regulatory documentation (ND) lacks such an indicator of the authenticity and good quality of the fruit as «microscopy». Despite the available data on the anatomy and histology of the fruits of this type of medicinal plant material (MPM), the scientific literature does not describe the luminescence features of the tissues of sea buckthorn fruit. It is known that the luminescent features of tissues make it possible to identify the localization of biologically active structures, and also, in some cases, to conduct selective diagnosis of MPM.Aim. The aim of this work was to study the characteristics of the luminescence of the tissues of dried crushed fruits of sea buckthorn, and also to optimize the conditions for analysis.Materials and methods. The object of the study was the dried, crushed fruits of sea buckthorn of various species. The study of microdiagnostic signs was carried out according to the State Pharmacopoeia of the XIV RF GPA.1.5.3.0003.15 «Technique of microscopic and microchemical studies of medicinal plant materials and herbal medicines». A stereomicroscopic study was carried out on a Biomed-6 microscope. The luminescence of sea buckthorn fruit tissues was examined using a luminescent microscope of the Micromed-3 Lum brand.Results and discussion. The most pronounced yellow glow is observed for the tissue of the pulp and epidermis of the fetus, which is associated with the highest content of fatty oil in these structures. Groups of phenolic compounds contained along with oil give a greenish tint to objects, while condensed tannins give a brownish tint. The plates that make up the corymbose hairs do not exhibit bright luminescence; a weak glow is characteristic of the joints of the hair platelets. The walls of stellate hairs have a faint greenish glow. Fragments of the actual fruit («sac») are brownish in color, without their own luminescence, as well as the seed peel. The embryo has its own greenish luminescence due to the presence of both a fatty oil and a complex of storage substances.Conclusion. The luminescent analysis of sea buckthorn fruits was carried out for the first time. The choice of a method for preparing the studied MPM for microscopic examination is experimentally substantiated. The luminescent analysis revealed the peculiarities of the glow of the tissues of the fruits of sea buckthorn. The main microdiagnostic signs of crushed dried sea buckthorn fruits and their biometric characteristics are specified. The analysis of the obtained data will allow us to further develop the section «Microscopic signs» for inclusion in the PA project on MPM, widely cultivated and used by the domestic pharmaceutical industry for the production of herbal medicines.

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Members of the family Lamiaceae Lindl. as sources of medicinal plant raw materials to obtain neurotropic drugs

Pharmacy & Pharmacology ◽

10.19163/2307-9266-2020-8-1-4-28 ◽

2020 ◽

Vol 8 (1) ◽

pp. 4-28

Author(s):

Е. V. Zvezdina ◽

J. V. Dayronas ◽

I. I. Bochkareva ◽

I. N. Zilfikarov ◽

E. Yu. Babaeva ◽

...

Keyword(s):

Medicinal Plant ◽

Scientific Literature ◽

Raw Materials ◽

Neurotropic Activity ◽

Biologically Active ◽

Plant Materials ◽

Plant Raw Materials ◽

Pharmaceutical Substances ◽

The Family ◽

Active Substances

The aim of this work is to review and analyze the data published in the modern scientific literature obtained in pharmacological, pharmacognostic and pharmacotechnological studies of various types of raw materials obtained from members of the family Lamiaceae L., which were sources of biologically active substances, pharmaceutical substances, total extracts and the drugs – with a neurotropic activity.Materials and methods. For the review, we used the information of scientific literature from open and accessible sources of the last twenty years, located in the scientific and technical libraries of institutions, as well as in electronic databases: Elibrary, PubMed, Scopus, Cyberleninka, GoogleAcademy, J-Stage. The search inquiries were: the species of the family Lamiaceae (Russian and Latin), the samples of medicinal plant materials based on them as well as the names of the drugs and biologically active substances obtained from these raw materials.Results. When working with the sources of scientific information, the main attention was paid to pharmacologic tests performed during the studies on laboratory animals and proving the presence of neurotropic activity in the studied objects – essential oils and extracts from plant raw materials: aqueous, aqueous alcoholic, and methanol ones. It has been established that the potential of the therapeutic and preventive application of pharmaceutical substances and drugs based on the medicinal plant materials obtained from 30 genera members of the Lamiaceae family, remains unrealized despite the close attention of various researchers.Conclusion. This review comprised 71 species from 30 genera. Despite the significant level of the previous study presented in the analysis of this publication, an enormous potential of this family’s species remains unexplored. In the future, they can be of both – pharmacognostic and practical interest, in particular, in creation of new medicinal preparations of the neurotropic action based on them.

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Hypericum Perforatum Grass: Chemical Composition, Properties, Use

Vestnik APK Verhnevolzh`ia ◽

10.35694/yarcx.2021.53.1.010 ◽

2021 ◽

pp. 57-63

Author(s):

И.Ю. Постраш

Keyword(s):

Chemical Composition ◽

Hypericum Perforatum ◽

Raw Materials ◽

Extraction Process ◽

Pharmaceutical Products ◽

The Body ◽

Biologically Active ◽

Quantitative Relation ◽

Resource Saving ◽

Two Phase

Трава зверобоя является ценным лекарственным растением, так как содержит большое число разнообразных биологически активных соединений, важнейшими из которых являются флавоноиды (1,5–5%), нафтодиантроны, дубильные вещества (10–13%), эфирное масло (0,1–0,33%) и др. Препараты, содержащие зверобой, комплексно воздействуют на организм, они оказывают противовоспалительное, антимикробное, антидепрессивное, противовирусное, кровоостанавливающее действие. Трава зверобоя применяется в виде чаёв, отваров, водных настоев, спиртовых и масляных настоек, мазей, сухих экстрактов. В ветеринарной медицине широко применяют настои и отвары травы зверобоя при заболеваниях ЖКТ: диспепсиях, гастроэнтеритах, острых и хронических колитах (КРС, овцы, козы, свиньи, собаки и др.), а также при лечении смешанных инвазий. Настои и настойку применяют также наружно при стоматитах, гингивитах, фарингитах. Несмотря на перечисленный выше ассортимент лекарственных форм зверобоя, активно ведутся научные исследования по оптимизации способов извлечения биологически активных веществ (БАВ) из травы зверобоя продырявленного. В ходе экспериментов исследователи варьируют различные параметры процесса экстракции: химический состав экстрагентов (монофазная и двухфазная экстракция), время и температуру экстракционного процесса, количественное соотношение сырья и экстрагентов, степень измельчения сырья, последовательность технологических операций, возможность применения излучения и др. Разработки новых ресурсосберегающих технологий переработки зверобоя продырявленного будут способствовать снижению себестоимости фармацевтической продукции и улучшению её фармакологических свойств. Hypericum perforatum grass is a valuable medicinal plant, since it contains a large number of various biologically active compounds, the most important of which are flavonoids (1.5–5%), naphthodianthrones, tannins (10–13%), aethereal oil (0.1–0.33%), etc. Preparations containing Hypericum perforatum have a complex effect on the body, they have an anti-inflammatory, antimicrobial, antidepressant, antiviral, hemostatic effect. Hypericum perforatum grass is used in the form of teas, apozems, water infusions, alcohol and oil tinctures, ointments, dry extracts. In veterinary medicine, infusions and decoctions of Hypericum perforatum grass are widely used in diseases of the gastrointestinal tract: dyspeptic disorder, gastroenteritis, acute and chronic colitis (cattle, sheep, goats, pigs, dogs, etc.), as well as in the treatment of mixed invasions. Infusions and tincture are also used externally for stomatitis, gingivitis, blennisthmia. Despite the above range of dosage forms of Hypericum perforatum, researches are actively conducted to optimize methods of extracting biologically active substances (BAS) from Hypericum perforatum grass. During the experiments, researchers vary various parameters of the extraction process: the chemical composition of extragents (monophasic and two-phase extraction), the time and temperature of the extractive process, the quantitative relation of raw materials to extragents, the dispersity of raw materials, the sequence of technological operations, the possibility of using radiation, etc. The development of new resource-saving technologies for processing Hypericum perforatum will help reduce the cost of pharmaceutical products and improve its pharmacological properties.

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