scholarly journals Synergistic Effect of Biofat and Biochar of Cashew Nutshell on Mitigate Methane in the Rumen

2020 ◽  
Vol 25 (3) ◽  
pp. 139
Author(s):  
Andi Saenab ◽  
Komang G Wiryawan ◽  
Y Retnani ◽  
Elizabeth Wina

The study aimed to evaluate the effectiveness of a combination of biofat with biochar or biosmoke (bioindustrial products of cashew nut shells) at the best level as feed additive in reducing methane production and improving in vitro rumen fermentation. This experiment had two series of combination and each used a randomized block design with 6 treatments and 4 replications. A series of biofat (BF) and biochar (BC) combination were added each to substrate as followed BFBC1 = 0: 100%; BFBC2 = 25:75%; BFBC3 = 50:50%; BFBC4 = 75:25%; BFBC5 = 100: 0%. While, a series of biofat (BF) and biosmoke (BS) combination as followed BFBS1 = 0: 100%; BFBS2 = 25:75%; BFBS3 = 50:50%; BFBS4 = 75:25%; BFBS5 = 100: 0%. Both series used a control treatment which contained only substrate. The in vitro experiment was repeated 4 times and each treatment was done in duplicates. The measured variables were: total gas and CH4 productions, dry matter, organic matter, NDF degradability, NH3 and partial VFA concentrations. The results showed that the combination of biofat and biochar levels resulted in a significant decrease (P<0.01) of CH4 production in the rumen. CH4 production was 88.50% (BFBC1), 63.15% (BFBC2), 61.50% (BFBC3), 58.16% (BFBC4) and 73.93% (BFBC5) compared to control treatment (100% CH4 production). The combination caused higher NH3 at BFBC4 and significantly higher propionate and total VFA in the rumen than control. Dry matter degradation values increased by a combination level biofat and biochar (BFBC4 and BFBC5), but these results were the same as control. Addition of combination of biofat and biosmoke caused a significant decrease (P<0.01) of CH4 production in the rumen. CH4 production was 71.98% (BFBS1), 65.57% (BFBS2), 64.81% (BFBS3),60.21% (BFBS4) dan 80.72 (BFBS5) compared to control treatment (100% CH4 production). At BFBS4 level, NH3 production, DMD and OMD values were lower than control. In conclusion, the best combination producing synergistic effect as feed additive to reduce methane and increase ammonia in the in vitro rumen was combination of biofat and biochar (BFBC4=75: 25%) or biofat with biosmoke (BFBS4= 75: 25%).

2014 ◽  
Vol 66 (3) ◽  
pp. 887-893 ◽  
Author(s):  
W.H.D. Buso ◽  
A.F.S. França ◽  
E.S. Miyagi

The bromatological composition and in vitro dry matter digestibility of millet cultivars were assessed for different nitrogen doses and two sowing seasons in the Ceres municipality of Goiás state, Brazil. The treatments consisted of three millet cultivars (ADR-7010, ADR-500 and BRS-1501), four nitrogen (N) doses (0, 50, 100 and 200kg ha-1 of N) and two sowing seasons. Three replicates and a randomised block design with a 3 x 4 x 2 factorial scheme were used. Two cuttings were performed in each season when plants reached an average height of 0.70 cm. No significant interactions were observed between or among cultivars for N doses and dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents. The DM, CP, NDF and ADF contents were significantly different between N doses. The DM and CP contents increased as the N dose increased to 100kg ha-1. The maximum DM and CP contents were 11.14 and 22.53%, respectively. The NDF and ADF contents were higher in the control treatment (60.11 and 30.01%, respectively). In addition, the lowest ADF and NDF concentrations occurred at an N dose of 50kg ha-1(56.33 and 30.23%, respectively). The DM contents were higher for the February sowing, with an average of 10.59%. The highest CP and ADF contents were found for the December sowing (22.46 and 31.58%, respectively). No significant differences were found for millet cultivars, N doses or sowing seasons. A significant interaction was found between sowing season and millet cultivar. The BRS-1501 cultivar had a higher in vitro dry matter digestibility in the December/2010 sowing (73.88%).


2022 ◽  
Vol 335 ◽  
pp. 00048
Author(s):  
Mashudi Mashudi ◽  
Wahyuni Nurmawati

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.


2014 ◽  
Vol 2 (2) ◽  
pp. 70
Author(s):  
Aang Baitul Mizan ◽  
Andi Murlina Tasse ◽  
Deki Zulkarnain

This study has conducted four weeks in Laboratory of Animal Nutrition Department of Animal Science, Faculty of Animal Science, Haluoleo University, Kendari. The treatments R0 = rations based feed’s non fermented, R1 = rations based 40% rice bran fermented (RBF), R2 = rations based 45% RBF, R3 = rations based 50% RBF, R4 = rations based 55% RBF, R5 = rations based 60% RBF. The experimental design used a randomized block design (RBD) with 5 treatments and 4 groups. The results showed that dry matter digestibility (DMD) of R1, R2, R5 higher than R0 (94,17%, 90,98%, 91,04% vs 88.60%). In contrast, DMD of R3 and R4 lower than R0 (80,90% and 80,89% vs 88,60%). Organic matter digestibility (OMD) of R0 lower than R1, R2, R3, R4 and R5 ( 62,58% vs 90,32%, 90,55%, 90,78%, 91,15% and 90,41%). In contrast, OMD of R4 higher than R1, R2, R3, R5 and R0 (91,41% vs 90,32%, 90,55%, 90,78%, 90,41% and 62,58%). Ammonia (NH3) concentrations of R1, R2, R3, R4 and R5 higher than R0 (8,20 mM, 5,40 mM, 8,67 mM, 7,22 mM and 7,42 mM vs 3,20 mM). The conclusion of this study was FBR based 40%, 45%, 50%, 55% and 60% RBF feasible to ruminant’s feed.Keywords : Feed’s Fermented, Digestibility, Dry Matter, Organic Matter, Ammonia.ABSTRAKPenelitian ini bertujuan mengevaluasi kelayakan penggunaan ransum berbasis pakan fermentasi untuk ternak ruminansia berdasarkan kecernaan bahan kering dan bahan orgaik serta protein secara In Vitro. Penelitian ini dilaksanakan selama empat minggu di Laboratorium Ilmu Nutrisi dan Makanan Ternak Fakultas Peternakan Universitas Haluoleo, Kendari. Perlakuan yang dicobakan adalah R0= ransum berbasis pakan non fermentasi (kontrol), R1= ransum berbasis dedak padi fermentasi 40%, R2= ransum berbasis dedak padi fermentasi 45%, R3= ransum berbasis dedak padi fermentasi 50%, R4= ransum berbasis dedak padi fermentasi 55%, R5= ransum berbasis dedak padi fermentasi 60%. Rancangan percobaan yang digunakan rancangan acak kelompok (RAK) dengan 5 perlakuan dan 4 kelompok. Hasil analisis ragam menunjukkan kecernaan bahan kering ransum berbasi dedak padi fermentasi 40%, 45%, dan 60% lebih tinggi dibanding dengan ransum berbasis pakan non fermentasi (94,17%; 90,98% dan 91,04% dibanding dengan 88,60%). Sebaliknya, ransum berbasis dedak padi fermentasi 50% dan 55% lebih rendah dibanding dengan ransum berbasis pakan non fermentasi (80,90% dan 80,89% dibanding dengan 88,60%). Kecernaan bahan organik ransum berbasis pakan non fermentasi lebih rendah dibanding dengan ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 55% dan 60% (62,58 % dibanding dengan 90,32 %, 90,55 %, 90,78 %, 91,15 % dan 90,41 % ). Sebaliknya kecernaan bahan organik ransum berbasis dedak padi fermentasi 55% lebih tinggi dibanding dengan ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 60% dan kontrol (91,41% dibanding dengan 90,32%, 90,55%, 90,78%, 90,41% dan 62,58%). Konsentrasi amonia ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 55% dan 60% lebih tinggi dibanding dengan ransum berbasis pakan non fermentasi (8,20 mM, 5,40 mM, 8,67 mM, 7,22 mM dan 7,42 mM dibanding dengan 3,20 mM). Kesimpulan bahwa ransum berbasis dedak padi fermentasi 40% hingga 60% layak digunakan untuk pakan ternak ruminansia.Kata kunci: Pakan Fermentasi, Kecernaan Bahan Kering, Kecernaan Bahan Organik, Amonia.


Author(s):  
Daniele Cristina PEREIRA ◽  
Rafael Henrique de Tonissi e Buschinelli de GOES ◽  
Antônio Campanha MARTINEZ ◽  
Jefferson Rodrigues GANDRA ◽  
Eduardo PRESENDO ◽  
...  

ABSTRACT This study evaluated the in vitro digestibility of nutrients from different diets added with chitosan (Q), technic cashew nut shell liquid (LCC) and the association of Q and LCC. The treatments used consisted of 4 diets (forage: concentrate ratio of 100: 0, 50:50, 40:60 and 20:80) associated with 4 additives (control, chitosan, LCC and the association of Q + LCC), totaling 16 treatments, in a 4x4 factorial randomized block design. The dosages used were: Control (without additives), LCC (600mg/kg DM), Chitosan (900mg/kg DM), and LCCQ (600mg/kg LCC DM + 900mg/kg Chitosan DM). In the laboratory, samples were analyzed for IVDMD, IVNDFD, IVCPD, pH and RAN (ruminal ammonia nitrogen). For pH and RAN analyses, samples were taken at 0, 2, 4, 6 and 8 hours after incubation. The results showed higher digestibility of DM, NDF and CP for diets with chitosan and technic cashew nut shell liquid alone and higher pH and RAN values in the diets containing the two additives. The association of additives brings better results for animal nutrition and increases ruminant productivity.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 404-404
Author(s):  
Leeroy A Lente ◽  
Roderick A Gonzalez Murray ◽  
Shawn L Archibeque

Abstract The aim of this study was to assess the effects of Zeolite (ZE, clinoptilolite) inclusion on in vitro rumen fermentation. A modified procedure from Tilley and Terry (1963) was used to determine alfalfa in vitro dry matter digestibility (IVDMD) in the presence or absence of ZE. Test tubes (n = 96) were placed in a 39 °C bath and were blocked based on hours 0, 1, 2, 4, 6, 8, 12, 24, and 48. Substrate consisted of dried alfalfa grinded through a 1mm screen using a Wily Mill. Treatments consisted of, Control (no ZE inclusion), and 0.05g ZE, for each 1 gram of alfalfa. After incubation, IVDMD was calculated 24 hours after drying period. Data was analyzed using a randomized block design. The IVDMD was found to be similar across all treatments (P &gt; 0.8464). The IVDMD was significantly different when incubated for the varying lengths of time (P &lt; 0.001). There was an IVDMD of 20.18 ± 2.89 % for the control and 18.07 ± 5.49 % for the ZE at the zero hour. The IVDMD for Control and ZE for 48 hours were 54.30 ± 1.58 % and 53. 48 ± 1.04 % respectively. To predict the IVDMD of the effects of ZE treatment on digestibility over time, the corresponding regression f(x) = 20.60 + 0.421X1 + 0.730X2 with R2 = 0.8464. In conclusion these data demonstrate that inclusion of ZE, does not influence the in vitro digestibility of alfalfa. These findings are important as previous data would indicate that inclusion of ZE may reduce cost of gain and/or influence liver abscess rates, it was uncertain if that would come at the cost of reduced digestibility of feed. These data indicate that there is likely very little to no impact on feed digestibility when ZE is included in the ration.


2015 ◽  
Vol 45 (12) ◽  
pp. 2229-2232
Author(s):  
Antonio Carlos Homem Junior ◽  
Jane Maria Bertocco Ezequiel ◽  
Henrique Leal Perez ◽  
Marco Tulio Costa Almeida ◽  
Josimari Regina Paschoaloto ◽  
...  

Methodological variations in the amount of sample incubated and the type of rumen fluid used are commonly observed. This study evaluated the effect of three sample amounts (0.6, 1.3 or 2.6g DM 100mL-1 of rumen fluid) incubated in rumen fluid buffered (BRF-buffered rumen fluid) or not (PRF-pure rumen fluid) on total gas volume (TV), methane (CH4) production, dry matter degradation (DM Deg) and final pH of corn silage incubated in vitro. The highest DM Deg was reached with the lowest amounts of sample (0.6 and 1.3g of DM 100mL-1 of rumen fluid). The sample amount of 2.6g associated with PRF reduced CH4 production (P<0.05), compared with the amounts of 0.6 and 1.3g DM 100mL-1, which had similar CH4 production (P>0.05). The use of BRF caused no effect on CH4 production (P>0.05), independent of the sample amount. Increasing the amount of substrate resulted in lower final pH of incubation in both fluids (P<0.05). Our results indicate that incubations should be performed with the smallest amount of sample (0.6g of DM 100mL-1 of rumen fluid), using fluid without buffer. Incubation without buffer solution overestimates the CH4 production of corn silage. Further studies should be conducted to verify the possibility of in vitro ruminal incubation of other ingredients using pure rumen fluid.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 432-432
Author(s):  
Adolfo Raramuri Méndez-Sánchez ◽  
Karen A Beauchemin ◽  
Luis Corona ◽  
Claudia Cecilia Márquez-Mota ◽  
Atmir Romero-Pérez

Abstract Tannins are natural compounds with potential to reduce enteric methane (CH4) emissions in ruminants. High-tannin sorghum (HTS) varieties are cultivated in different regions of the world, including Mexico. The objective of this study was to evaluate the effects of HTS on rumen fermentation and CH4 production using a batch culture system. The experiment was set up as a completely randomized block design using a 3 × 5 factorial arrangement with 3 grains [HTS, low-tannin sorghum (LTS), and corn, with 19.6, 0.07 and 0 g of condensed tannins (CT)/kg dry matter (DM) respectively] and 5 grain to forage (alfalfa hay) proportions (20:80, 40:60, 60:40, 80:20 and 100:0). Data were analyzed using the MIXED procedure of SAS. Contrasts were used to determine linear and quadratic effects of grain:forage proportions. A decrease of 2.96% in CH4 concentration was observed for sorghum treatments compared to corn (P &lt; 0.01). However, HTS and LTS were not different from each other (P = 0.76). Furthermore, CH4 production (mL/d) decreased (P &lt; 0.01) by 19.13 and 8.53% with HTS compared with corn and LTS respectively. This reduction was associated with a decrease (P &lt; 0.01) of 14.52 and 10.04% in total gas production with HTS as compared to corn and LTS. Both, CH4 and gas production decrease, were associated with reduced DM digestibility for HTS, which can be a negative effect of CT in HTS. Sorghum treatments produced 14.56% less CH4 (mL/g DM; P &lt; 0.01) than corn. This was partially explained by a 7.98% decrease in DMD with sorghum treatments. As expected, gas production linearly increased and rumen pH linearly decreased with increasing levels of grain (P &lt; 0.01). In conclusion, HTS had no advantage over LTS in terms of CH4 production. Nonetheless, sorghum grain, substituted for corn, can be an option to decrease CH4 production in ruminants, but digestibility might be compromised.


2015 ◽  
Vol 87 (4) ◽  
pp. 2301-2307 ◽  
Author(s):  
MATHEUS G. SILVA ◽  
ORIVALDO ARF ◽  
PAULO E. TEODORO

ABSTRACT The objective of this study was to investigate the effect of interaction between nitrogen topdressing and different application ways (active ingredients) a.i. fluazifop-p-butyl + fomesafen in weed control and agronomic performance of common bean. The experiment was conducted during winter 2003 in Selvíria/MS. The experimental design used was a randomized block design with four replications in a factorial scheme 2x7. The first factor was composed by the absence or presence of nitrogen topdressing, while the second factor consisted of different application ways of fluazifop-p-butyl + fomesafen. The following variables were measured: leaf N content, dry matter of plants, yield components (number of pods plant-1, number of grains plant-1, the average number of grains pod-1 and mass of 100 grains), grain yield, phytotoxicity and weed control percentage. The nitrogen topdressing with 75 kg ha-1provided higher dry matter of plants, higher weed control and higher common bean yield of irrigated winter. In the absence of nitrogen topdressing in the application of urea before or together to fluazifop-p-butyl + fomesafen increased their effectiveness in controlling weeds without interference in the agronomic performance of common bean.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 469-470
Author(s):  
Sandra Suescun-Ospina ◽  
Nelson Vera ◽  
Rita Astudillo ◽  
Jorge Avila-Stagno

Abstract País Grape (Vitis vinifera L.) is an ancestral variety used in Chilean wine industry. It has a higher content of proanthocyanidins than commercial varieties such as Carmenère or Pinot Noir, resulting in País grape marc (PGM) with high contents of condensed tannins. As such, PGM inclusion in ruminant diets would have the potential to reduce enteric methane (CH4) emissions and decrease urinary N excretion. The objective of this study was to evaluate the effects of substitution of mixed hay (MH) with PGM in a high concentrate diet [65% dry matter (DM)] on in vitro dry matter disappearance (IVDMD), ruminal fermentation parameters, gas and CH4 production. Treatments were: T1 (Control) = 20% MH, 15% corn silage, 65% concentrate; T2 = 10% MH, 10% PGM, 15% corn silage, 65% concentrate; T3 = 20% PGM, 15% corn silage, 65% concentrate. The study was a randomized complete design with 3 treatment and 3 replicates, incubated for 24 h at 39º C. Data were compared by Tukey test and polynomial contrasts. There was a linear reduction in NH3-N (P = 0.001) as dietary PGM increased. Inclusion of PGM reduced NH3-N by 50% when added at 10% DM, and 71.7% at 20% DM. However, there also was 4% reduction in IVDMD (P ≤ 0.001) and gas production (P = 0.012) in the 20% GM diet. There were no treatment effects (P ≥ 0.05) on CH4 production or yield. Fermentation efficiency determined by the partition factor increased linearly (P = 0.013) as PGM inclusion increased, suggesting that it increases organic matter to be degraded. Based on this study it could be concluded that PGM is an alternative source of fiber for ruminants on concentrate diets, as it can result in improved rumen fermentation efficiency and a substantial reduction in ruminal ammonia nitrogen concentration (NH3-N).


2010 ◽  
Vol 39 (8) ◽  
pp. 1666-1675 ◽  
Author(s):  
Marcio Mahmoud Megda ◽  
Francisco Antonio Monteiro

The objective of this work was to study morphogenic characteristics, and dry matter production of roots and shoots of marandu palisadegrass (Brachiaria brizantha cv. Marandu) submitted to combinations of nitrogen and potassium, in a nutritive solution, employing silica as substrate. The experiment was carried out in a greenhouse during the summer. It was used a 5² fractionated factorial scheme with 13 combinations of nitrogen and potassium, which were distributed in a randomized block design, with four replications. The nitrogen × potassium interaction was significant for the number of tillers and leaves, for leaf area, for shoots and root section dry mass, for total length and surface and specific length and surface in the roots. Production of aerial part dry mass positively correlated with the number of tillers and leaves and grass leaf area. Nitrogen rates modulated the root system development, and the root specific length and surface decreased when high rates of nitrogen and potassium were supllied. Nitrogen and potassium influence Marandu palisadegrass morphogenic characteristics, which are determinant for grass dry matter production.


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