Estimation of Na\K - ATPase Enzyme Activity and Endogenous Digitalis in Patients with Diabetic Neuropathy

2021 ◽  
Vol 19 (5) ◽  
pp. 95-103
Author(s):  
Eman Hameed Al-Rikabi ◽  
Mazin J. Mousa ◽  
Oda M. Yasser
Keyword(s):  
Enzyme Activity ◽  
Diabetic Neuropathy ◽  
Inhibitory Activity ◽  
Strong Association ◽  
Clinical Manifestations ◽  
Gradual Loss ◽  
Study Results ◽  
Healthy Control ◽  
The Mean ◽  
Age Range

Background: Among the most common complications of diabetes is diabetic neuropathy (DN). Diabetic neuropathy is a heterogeneous group of disorders, which involves a different part of somatic and autonomic nervous systems, with a gradual loss of neural conductivity. Some studies have shown that they reduce the activity of the Na/K ATPase, however, elevated levels of endogenous sodium pump inhibitor in diabetic individuals, including those with neuropathy. Changes in this transfer enzyme are believed to be due to several diabetes complications. Objective: The study had designed to evaluate the Na/K ATPase enzymatic activity in the erythrocyte-membrane among three groups. The first group had represented the patients with type 2 diabetes mellitus (DM2) and neuropathy. The second group is diabetics without neuropathy. The third group was a healthy subject. As well, the study had estimated the inhibitory activity of endogenous digitalis among patient groups. Furthermore, the aim of this research was to see whether there was a connection between red blood cell membrane Na-K ATPase activity and the medical facts of the analysis subjects. Design and Methods: One-hundred fifty subjects had enrolled in this case-control study; 80 patients complained of diabetic neuropathy of both sexes, the mean age 59.3 years with an age range of 40-81, 40 DM2 without neuropathy (53.9 years), (35 – 70), and 30 healthy controls (30 years, 25 to 45). Patients in the first group were selected carefully according to their clinical manifestations and the nerve conduction study results. The evaluations of both inhibitory activities of endogenous digitalis and Na/K ATPase had completed using a spectrophotometer. Enzyme activity had expressed in micrograms of phosphate concentration per grams of red cell ghost total protein concentration. Results: The mean enzyme activity of Na/K ATPase was significantly lower (p<0.001) in patients with diabetic neuropathy (381±17.9) compared with the diabetic group without neuropathy (498±22.9) and the normal controls (837±61.43). There was a significant inhibitory activity of endogenous digitals (17.87±2.15) in patients with DNP, compared with the diabetics without neuropathy (8.78±0.89) and healthy control (5.3±1.33). There was a significant association of enzyme activity with the following parameters: duration of diabetes, age, level of glycated hemoglobin and endogenous digitalis with the respective p-values (0.000, 0.000, 0.000 and 0.021). Gender showed no significant relationship with enzyme activity (p 0.43). Conclusions: In DM2 with neuropathy, hyperglycemia can much reduce the activity of erythrocyte Na/K ATPase. In addition, it may enhance the inhibitory activity of endogenous digitals. The timedependent increase in diabetic complications can be due to a strong association between diabetes duration and erythrocyte Na/K-ATPase activities.

scholarly journals Study of the Immunological Status of Iraqi Vitiligo Patients

2016 ◽  
Vol 13 (3) ◽  
pp. 454-462
Author(s):  
Baghdad Science Journal
Keyword(s):  
Potential Role ◽  
Immunoglobulin E ◽  
Ultraviolet B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Serum ◽  
Healthy Control ◽  
The Mean ◽  
Age Range ◽  
Apparently Healthy

Vitiligo is an acquired idiopathic skin disorder characterized by depigmented macules due to loss of cutaneous melanocytes. A potential role of the immune dysfunction has been suggested in vitiligo, so to test this hypothesis, certain cytokines (IL-17A and TNF-?) and immunoglobulins (IgM, IgG, IgA and total IgE) were investigated in all participants. The study included: 60 patients with age range between (6-55) year; 30(11 males and 19 females) were untreated and 30(12 males and 18 females) were treated with Narrow Band Ultraviolet-B (NB-UVB) and 30 (14 males and 16 females) apparently healthy control. Serum was separated and cytokines (IL-17A and TNF-?) and total immunoglobulin E (IgE) were detected by using Enzyme Linked Immunosorbent Assay (ELISA); while immunoglobulins (IgM, IgG and IgA) were detected by using Single Radial Immunodiffusion (SRID) method. The results showed that the mean levels of serum IL-17A and TNF-? in both untreated and NB-UVB treated vitiligo patients were increased significantly (p ? 0.05) as compared with healthy control. The mean levels of serum IgG and IgA in untreated vitiligo patients showed non significant decreased (P

scholarly journals Investigation of JC Polyomavirus (JCV) Genome in Colorectal Cancer Patients from Iran

2020 ◽  
Author(s):  
Mohammad Hadi KARBALAIE NIYA ◽  
Mohsen KESHAVARZ ◽  
Fahimeh SAFARNEZHAD TAMESHKEL ◽  
Mahsa TAHERIZADEH ◽  
Maryam ESGHAEI ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Positive Result ◽  
Real Time ◽  
Real Time Pcr ◽  
Positive Control ◽  
Melting Curve Analysis ◽  
Jc Polyomavirus ◽  
Study Results ◽  
Healthy Control ◽  
The Mean

Background: JC polyomavirus (JCV) is an epitheliotropic and neurotropic virus that identified in relationship with some devastating complications such as progressive multifocal leukoencephalopathy (PML) and linked to colorectal cancer. The aim of current study was to identify the prevalence of JCV in colorectal cancer for the first time in Iran. Methods: This retrospective case-control study was conducted by the hospitals affiliated to Iran University of Medical Sciences, Tehran, Iran from 2011 to 2016. Formalin-fixed paraffin-embedded (FFPE) blocks were used for DNA extraction by QIAamp® DNA FFPE Tissue Kit. The SYBER Green Real-time PCR assay performed by specific primers for JCV T-Large Ag. Melting curve analysis used for evaluation of amplification specificity. Positive control cloned in pTZ57R/T plasmid by Generay Biotechnology system. Results: Of 157 specimens 66 were colorectal cancer by the mean age (y) ± std. deviation 59.35±14.48 and 91 healthy control by the mean age (y) ± std. deviation 57.21±14.66. All 157 specimens tested for JCV T-Large Ag gene by Real-time PCR method and we found that there was not any positive result although the melting analysis showed specificity of positive control amplification. Conclusion: Low prevalence of JCV infection in Iranian CRC population confirmed by the current study results; there was not any JCV positive result in CRC and healthy control groups. Further studies by broader and different populations are recommended.

scholarly journals Lower Limb Varicose Veins's Clinical Profile and Management

2020 ◽  
pp. 1-5
Author(s):  
C. Z. Perdeshi ◽  
Kustub A. Kulkani ◽  
Revendra N. Yadav ◽  
Mayur Nagwakar ◽  
Niten H. Patil
Keyword(s):  
Varicose Vein ◽  
Lower Limb ◽  
Varicose Veins ◽  
Clinical Manifestations ◽  
Clinical Profile ◽  
Lower Limbs ◽  
Study Results ◽  
Common Technique ◽  
Age Range ◽  
Made In

The lower limbs’ venous system has the pressure of posture, and blood has to be pushed against gravity into the heart cavity. This issue is generally approached either by a cautious approach or by surgical interference, all of which are constrained. Attempts to study different clinical manifestations of varicose veins are being made in the present study. The overall number of 50 varicose vein patients was analysed and the study results were reported. Varicosity veins of the lower limb is a fairly normal pathological entity. In the 20-50 age range, the condition is more common. The main modality of the procedure is surgery. The most common technique performed is Saphenofemoral flush ligation with stripping. 

scholarly journals Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease

2017 ◽  
Vol 14 (3) ◽  
pp. 557-563
Author(s):  
Baghdad Science Journal
Keyword(s):  
Enzyme Activity ◽  
Celiac Disease ◽  
Alanine Aminotransferase ◽  
Mean Value ◽  
Control Group ◽  
Serum Samples ◽  
Healthy Group ◽  
The Mean ◽  
Age Range ◽  
Alt Activity

Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p

scholarly journals Genetic Polymorphisms rs643627 in Serotonin Receptor Gene (5-HTR2A) with Schizophrenia

2019 ◽  
pp. 2642-2648
Author(s):  
Noor K. Khudhiar ◽  
Asmaa M. Saud
Keyword(s):  
Serotonin Receptor ◽  
Receptor Gene ◽  
Demographic Data ◽  
Genotype Frequencies ◽  
Gender And Age ◽  
Study Results ◽  
Healthy Control ◽  
Intron Region ◽  
And Gender ◽  
Age Range

Schizophrenia(SCZ) is oneof the most destructive and complicated chronic diseases of the human nervous system. Serotonin receptors have been involved in the pathophysiology of psychiatric disorders including schizophrenia. Fortyschizophrenia subjects (14females and 26 males) with an age range of 23– 57 years were enrolled, in addition to twenty healthy control subjects (10female and 10 male) with an age range of 19-44 years. This study aimed to evaluate the frequency of one single nucleotide polymorphism (SNP), namelyrs643627 in HTR2Agene,inIraqi patients with schizophrenia in comparison with controls, along with the association between this SNP and the incidence of schizophrenia. The genetic variantrs643627 within the intron region of 5-HTR2Agene was genotyped by Real Time –PCR. The results showed that differences in the demographic data of gender and age between schizophrenia subjects and controls were statically non-significant. Also, the genotype frequencies distribution of rs643627 polymorphism showed no deviation from Hardy–Weinbergequilibrium in both groups (patients and controls). In addition,differences in the genotypes(AA, AG, and GG) and allele frequencies of 5-HTR2A were statically non-significant between SCZ patients and controls. However,the present study results demonstrated an association between rs643627 polymorphism of 5-HT2Ageneand age and gender in schizophrenia patients group.

RESULTS OF TREATMENT PNEUMONIA FOR CHILDREN FROM 2 MONTHS TO 5 YEARS OLD AT YEN PHONG HEALTH CENTER, PROVINCE BAC NINH

2021 ◽  
Vol 62 (6) ◽  
Author(s):  
Nghiem Thi Dung ◽  
Nguyen Thanh Trung ◽  
Tran Thi Thuy ◽  
Tao Thi Hong Van
Keyword(s):  
Health Center ◽  
Clinical Manifestations ◽  
Antibiotic Use ◽  
Cure Rate ◽  
Duration Of Treatment ◽  
Results Of Treatment ◽  
Study Results ◽  
The Mean ◽  
Combination Antibiotics ◽  
Common Combination

Objectives: To evaluate results of treatment pneumonia for children aged 2 months to 5 years in pediatric deapartment at Yen Phong health center, Bac Ninh. Subjects: Patients with pneumonia aged 2 months to 5 years for treatment from September 2020 to June 2021. Methods: Descriptive study. Results: A total of 146 children were diagnosed with pneumonia, of which 82 were male (56.3%). The mean age was 20.6 ± 14.2 months. Prevalence of antibiotic use in the pre-hospital was 69.9%. The most common clinical manifestations were cough (100.0%), a subcrepitant rales (100.0%) and fever (29.5%). The most common X-ray was widespread patchy infiltrates (61.0%). 77.4% of children used combination antibiotics, in which the most common combination was Cephalosporin + Aminoglycoside (67.8%). Average duration of treatment was 6,7 ± 2,2 days. The cure rate was 100%. Conclusion: Pneumonia is more common in children less than 1 year old, with clinical manifestations of cough, shortness of breath and a subcrepitant rales. Combination antibiotics are effective in treating pneumonia in young children.

Regional Cerebral Blood Flow (rCBF) in Migraine During the Interictal Period: Different rCBF Patterns in Patients With and Without Aura

Cephalalgia ◽  
1996 ◽  
Vol 16 (3) ◽  
pp. 161-168 ◽  
Author(s):  
E Facco ◽  
M Munari ◽  
F Baratto ◽  
AU Behr ◽  
A Dal Palu ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Regional Cerebral Blood Flow ◽  
Migraine Without Aura ◽  
Tension Type Headache ◽  
Regional Cerebral Blood ◽  
Healthy Control ◽  
The Mean ◽  
Type Headache ◽  
Age Range

The aim of this study was to evaluate the rCBF (133Xe clearance method) in migrainous patients free from attack. Fifty patients suffering from migraine without aura (group M) and 20 suffering from migraine with aura (group MA) (age range 20-50 years) were submitted to 32 channel rCBF mapping during the interictal period. The rCBF data of patients were compared with those obtained from 60 healthy control subjects (group C) and 21 patients suffering from tension-type headache (group TH). The mean (average of all channels) rCBF values were: group M=70.5 ± 13.7ml/100g/min; group MA=56.6 ± 11.4ml/100g/min; group C=62.3 ± 8.3ml/100g/min; group TH=62.1 ± 8.4ml/100g/min (F=11.93; p<0.001). As expected, patients belonging to group TH had a normal rCBF. The mean rCBF of group M was significantly higher than that of groups C and TH, while in group MA it was significantly lower than in groups C and TH. Group M showed a diffuse hyperemia, while group MA showed rCBF values significantly lower than normal in posterior regions, according to aura. Our results suggest that: (a) the rCBF pattern in migrainous patients is different from that in both controls and TH patients, even during the interictal period; (b) patients suffering from migraine with and without aura are two distinct subpopulations with opposite rCBF deviations.

scholarly journals Early epidemiological and clinical manifestations of COVID-19 in Japan

2020 ◽  
Author(s):  
Muhammad Qasim ◽  
Muhammad Yasir ◽  
Waqas Ahmad ◽  
Minami Yoshida ◽  
Muhammad Azhar ◽  
...  
Keyword(s):  
Clustering Algorithm ◽  
Clinical Manifestations ◽  
Sudden Increase ◽  
Rt Pcr ◽  
Travel History ◽  
Main Cluster ◽  
Community Transmission ◽  
The Mean ◽  
Age Range ◽  
Epidemiological Parameters

AbstractBackgroundSevere acute respiratory syndrome coronaviruses -2 (SARS-COV2) named as COVID-19 had spread worldwide and leading to 1,210,956 confirmed cases and 67,594 deathsMethodsA data of 1192 confirmed cases and 43 deaths due to COVID-19 in Japan collected from the Ministry of Health, Labour and Welfare of Japan and analysed for different epidemiological parameters and their clinical manifestations. We used Clauset-Newman-Moore (CNM) clustering algorithm to develop web-network of confirmed cases to identified clusters of community transmission.ResultsOut of 1192 confirmed cases, 90.60% were symptomatic and 9.39% were asymptomatic. The prevalence of COVID19 in males was 56.29% and 43.20 % in females. The mean interval (±SD) from symptom onset to diagnosis was 6±22.6 days while mean interval (±SD) from contact to onset of symptoms was 5±19.5 days. People of age range 40-79 were more infected and deaths median age was 80. The main symptoms were fever, dry cough, fatigue and pneumonia. The main infected cities were Tokyo (195/1192, 16.35%), Hokkaido (160/1192 13.42%), Aichi (150/1192, 12.58%) and Osaka (145/1192, 12.16%). Only 2.34% cases had travel history from Wuhan China and Osaka music concert was identify as main cluster for community transmission. While 556 (46.64%) cases were clinically diagnosed and 557 (46.72%) were confirmed by using RT-PCR.ConclusionsOther than, declare emergency Japan need to change their approach of diagnosing COVID-19, as asymptomatic cases prevalence is high and maybe it is reason for current sudden increase of cases. Screening centre should be establish away from hospitals, which are treating positive cases.

scholarly journals Lower Urinary Tract Symptoms in Myasthenia Gravis

2021 ◽  
pp. 490-498
Author(s):  
Fuyuki Tateno ◽  
Ryuji Sakakibara ◽  
Yosuke Aiba
Keyword(s):  
Urinary Tract ◽  
Myasthenia Gravis ◽  
Lower Urinary Tract ◽  
Pelvic Organ ◽  
Control Group ◽  
Control Subjects ◽  
Study Results ◽  
Healthy Control ◽  
Age Range ◽  
Lower Urinary

It remains uncertain to what extent lower urinary tract (LUT) symptom (LUTS) is a comorbidity of myasthenia gravis (MG). We prospectively administered a LUTS questionnaire devised for detecting neurogenic pelvic organ dysfunction (not validated) in an MG group and a healthy control group and compared the results. The MG group comprised 21 patients: 15 women and 6 men, with age range 22–73 (mean 47) years, illness duration range 0.2–8 (mean 3.5) years, median Myasthenia Gravis Foundation of America (MGFA) grade 2, all walking independently. Therapies included thymectomy in 17, predonisolone 5–20 mg/day in 10, and pyridostigmine bromide 60–180 mg/day in 9 patients. The control group, who were undergoing an annual health survey, comprised 235 consecutive subjects: 120 women and 115 men, with age range 30–69 (mean 48) years. The questionnaire had 9 questions. Each question was scored from 0 (none) to 3 (severe) with an additional quality of life (QOL) index scored from 0 (satisfied) to 3 (extremely dissatisfied). Statistical analysis was made using Student’s <i>t</i> test. Compared with the control subjects, the frequency of LUTSs in the MG patients was significantly higher for daytime frequency (43%; <i>p</i> &#x3c; 0.01), nocturia (24%; <i>p</i> &#x3c; 0.01), and urinary incontinence (43%; <i>p</i> &#x3c; 0.05). The LUTS-related QOL index for the MG patients was significantly higher for MG patients as a whole than that for all control patients (29%) (<i>p</i> &#x3c; 0.05). In conclusion, our study results showed that MG patients had significantly more LUTSs (overactive bladder) than healthy control subjects and had worse LUTS-related QOL; therefore, amelioration of LUTS in MG is important.

Vitamin D Treatment in Patients with Hashimoto’s Thyroiditis may Decrease the Development of Hypothyroidism

2016 ◽  
Vol 86 (1-2) ◽  
pp. 9-17 ◽  
Author(s):  
Bekir Ucan ◽  
Mustafa Sahin ◽  
Muyesser Sayki Arslan ◽  
Nujen Colak Bozkurt ◽  
Muhammed Kizilgul ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Hashimoto’S Thyroiditis ◽  
Healthy Control Group ◽  
Hashimoto's Thyroiditis ◽  
Control Group ◽  
Endocrine Society ◽  
Thyroid Autoantibody ◽  
Healthy Control ◽  
The Mean

Abstract.The relationship between Hashimoto’s thyroiditis and vitamin D has been demonstrated in several studies. The aim of the present study was to evaluate vitamin D concentrations in patients with Hashimoto’s thyroiditis, the effect of vitamin D therapy on the course of disease, and to determine changes in thyroid autoantibody status and cardiovascular risk after vitamin D therapy. We included 75 patients with Hashimoto’s thyroiditis and 43 healthy individuals. Vitamin D deficiency is defined as a 25-hydroxy vitamin D (25(OH)D3) concentration less than 20ng/mL. Vitamin D deficient patients were given 50.000 units of 25(OH)D3 weekly for eight weeks in accordance with the Endocrine Society guidelines. All evaluations were repeated after 2 months of treatment. Patients with Hashimoto’s thyroiditis had significantly lower vitamin D concentrations compared with the controls (9.37±0.69 ng/mL vs 11.95±1.01 ng/mL, p < 0.05, respectively). Thyroid autoantibodies were significantly decreased by vitamin D replacement treatment in patients with euthyroid Hashimoto’s thyroiditis. Also, HDL cholesterol concentrations improved in the euthyroid Hashimoto group after treatment. The mean free thyroxine (fT4) concentrations were 0.89±0.02 ng/dL in patients with Hashimoto’s thyroiditis and 1.07±0.03 ng/dL in the healthy control group (p < 0.001). The mean thyroid volumes were 7.71±0.44 mL in patients with Hashimoto’s thyroiditis and 5.46±0.63 mL in the healthy control group (p < 0.01). Vitamin D deficiency is frequent in Hashimoto’s thyroiditis and treatment of patients with this condition with Vitamin D may slow down the course of development of hypothyroidism and also decrease cardiovascular risks in these patients. Vitamin D measurement and replacement may be critical in these patients.

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