scholarly journals Efek pemberian ekstrak klorofil daun pepaya (carica papaya, linn.) terhadap kadar ast dan alt serum

2016 ◽  
Vol 4 (1) ◽  
pp. 63-66
Author(s):  
Kusmadewi Eka Damayanti ◽  
Noor Wijayahadi ◽  
Niken Puruhita
Keyword(s):  
Wistar Rats ◽  
Pharmacological Therapy ◽  
Group Iv ◽  
Control Group ◽  
High Dose ◽  
Group Iii ◽  
Group I ◽  
Post Hoc ◽  
Agent Group ◽  
Chlorophyll Extracts

Background: Pharmacological therapy is one of the therapy modalities which carries the hepatic injury as one of its side effects. Nowadays, curcuma tablets has become choice of hepatoprotector. Other substance which has the possibilities of hepatoprotection activities is chlorophyll. The study is aimed to investigate the effect of papaya leaves chlorophyll extracts towards the serum AST and serum ALT levels on high-dose-paracetamol-induced Wistar rats. Materials and methods: This was an experimental study applying pretest-posttest controlled group design. Twenty eight Wistar rats 8-12 weeks age and weighted 185-220 grams assigned into four groups, namely group I (control), group II (paracetamol 750 mg/kg bw + no hepatoprotector agent), group III (paracetamol 750 mg/kg bw + curcuma 100 mg/kg bw), and group IV (paracetamol 750 mg/kg bw + papaya leaves chlorophyll extracts 300 mg/kg bw). The hepatoprotector agents were administered for a week, while the high dose paracetamol was administered for three consecutive days (day 5, 6, and 7). The pretest samples were drawn on the fifth day before the administration of high dose paracetamol, and the posttest samples were drawn on eightth day. Results: There were no difference among four groups on serum AST and serum ALT levels before the administration of high dose paracetamol, p=0.522 dan p=0.682, respectively. After the administration of high dose paracetamol, there were differences among four groups on both variables, both p=0.000. The post-hoc test showed that differences happened on all four groups (p<0.05).Conclusion: Chlorophyll extract of papaya leaves can inhibit liver injury on high-dose-paracetamol-induced-Wistar rats. 

scholarly journals Diosmin Alleviates Doxorubicin-Induced Liver Injury via Modulation of Oxidative Stress-Mediated Hepatic Inflammation and Apoptosis via NfkB and MAPK Pathway: A Preclinical Study

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1998
Author(s):  
Abdullah F. AlAsmari ◽  
Metab Alharbi ◽  
Faleh Alqahtani ◽  
Fawaz Alasmari ◽  
Mohammed AlSwayyed ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Mapk Pathway ◽  
Preclinical Study ◽  
Group Iv ◽  
Control Group ◽  
High Dose ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Pre Treatment

Hepatotoxicity caused by chemotherapeutic drugs (e.g., doxorubicin) is of critical concern in cancer therapy. This study focused on investigating the modulatory effects of diosmin against doxorubicin-induced hepatotoxicity in Male Wistar rats. Male Wistar rats were randomly divided into four groups: Group I was served as control, Group II was treated with doxorubicin (20 mg/kg, intraperitoneal, i.p.), Group III was treated with a combination of doxorubicin and low-dose diosmin (100 mg/kg orally), and Group IV was treated with a combination of doxorubicin and high-dose diosmin (200 mg/kg orally) supplementation. A single dose of doxorubicin (i.p.) caused hepatic impairment, as shown by increases in the concentrations of serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase. Doxorubicin produced histological abnormalities in the liver. In addition, a single injection of doxorubicin increased lipid peroxidation and reduced glutathione, catalase, and superoxide dismutase (SOD) levels. Importantly, pre-treatment with diosmin restored hepatic antioxidant factors and serum enzymatic activities and reduced the inflammatory and apoptotic-mediated proteins and genes. These findings demonstrate that diosmin has a protective effect against doxorubicin-induced hepatotoxicity.

scholarly journals Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Akheruz Zaman Ahmed ◽  
Shakta Mani Satyam ◽  
Prakashchandra Shetty ◽  
Melanie Rose D’Souza
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Methyl Gallate ◽  
Group Iii ◽  
Group I ◽  
Female Wistar Rats ◽  
Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p  < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p  < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

scholarly journals PRENATAL AND POSTNATAL EFFECT OF LEAD ACETATE ON THE HISTOLOGY OF FRONTAL CORTEX AND MOTOR ACTIVITY IN WISTAR RATS

2021 ◽  
pp. 10-12
Author(s):  
SALEH NUHU ◽  
HAUWA IDRIS AHMAD ◽  
AISHA MUHAMMAD GARBA ◽  
TASIU ABDULLAHI SULAIMAN
Keyword(s):  
Drinking Water ◽  
Motor Activity ◽  
Frontal Cortex ◽  
Wistar Rats ◽  
Histological Study ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Objectives: The objective of this study was to find the histologic and motor activity effect of lead on prenatally and postnatally exposed Wistar rats. Methods: In this study, twelve Wistar Rats were used and grouped into four groups of two females and one male. Group I rats served as the control and allowed feed and water freely. The rats in Group II were administered 500ppm of Pb through drinking water from gestation day 8 (GD8) to parturition (GD21). While Group III rats were given 500ppm of Pb in drinking water from postnatal day 1 (PND1) to PND21. The rats in the fourth group (Group IV) were given 500ppm of Pb from GD8 to PND21. Palmer grasp reflex was conducted to assess the motor activity of the rat pups. The animals were then humanely sacrificed and the frontal cortices were isolated for routine histological processing. Results: The histological study has shown normal neurons in the control group while degenerating cells exhibiting karyolysis, pyknosis, karyorrhexis, vacuolation were seen in the lead-treated groups. Group II and Group IV showed considerate deficit in their motor activity while Group III showed mild effect. Conclusion: From this study, lead exposure of Wistar rats at both prenatal and postnatal period of development has effect on the histology of the frontal cortex as well as on their motor activity.

scholarly journals Comparison of health supplement on strength, stamina and growth of rats

2019 ◽  
Vol 8 (4) ◽  
pp. 806
Author(s):  
Swanand S. Pathak ◽  
Nikhil S. Yadav
Keyword(s):  
Wistar Rats ◽  
Tail Length ◽  
Maximum Growth ◽  
Group Iv ◽  
Control Group ◽  
Natural Health ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Health Supplements

Background: Children require more nutrition as organs grow fast in this age group. Due to abundance of various commercial health supplements in market, parents are often confused about selection of health supplements. Traditional foods are rich source of various nutrients required by growing children. We conducted a study to compare strength, stamina and growth enhancing ability of commercial health supplement and natural health supplement on wistar rats.Methods: Total of 24 wistar rats were included. Animals were divided in four groups. Each group of rats except control group were receiving health supplements. Group I, group II, group III and group IV were receiving health supplement I, health supplement II, natural health supplement and no health supplement (control group) for 60 days. Strength and stamina of wistar rats in each group were analysed using grip strength meter and swim stress test, growth of rats in each group were analysed by measuring body length, tail length and body weight. Group of rat showing maximum growth (body length, tail length and body weight), strength and stamina were analysed.Results: Group II rats showed greater strength and stamina followed by group I, group III and group IV respectively. Group I rats showed maximum growth followed by group II, group III and group IV respectively.Conclusions: In the current study we found that strength and stamina were greater in group II rats followed by group I, group III and group IV respectively also group I rats showed maximum growth  followed by group II, group III and group IV respectively.

scholarly journals Prophylactic and Curative Assessment of Essentiale Forte® On Carbon Tetrachloride-Induced Liver Damage in Wistar Rats

2014 ◽  
Vol 2 (3) ◽  
pp. 408-414 ◽  
Author(s):  
Olaoluwa S Olukiran ◽  
Rufus O Akomolafe ◽  
Kayode O Bamitale ◽  
Akande O Ajayi ◽  
Raphael E Okonji ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Wistar Rats ◽  
Recovery Period ◽  
Total Bilirubin Level ◽  
Group Iv ◽  
Control Group ◽  
Active Period ◽  
Group Iii ◽  
Group I

AIM: This study was to assess the prophylactic and curative effects of Essentiale forte (ESF) on carbon tetrachloride (CCl4) induced liver damage in Wistar rats.MATERIALS AND METHODS: Twenty-four adult Wistar rats were randomly divided into four groups of six rats each. Group I (control group) received 0.3 ml/kg/day of propylene glycol for one month; group II (toxic control) was given 0.7 ml/kg/day of CCl4 dissolved in olive oil (1:1,v/v) orally for 7 days; group III (prophylactic group) received 4.3 mg/kg/day of ESF for one month followed by CCl4 for one week; group IV (curative group) was treated with CCl4 for one week and subsequently received ESF (4.3 mg/kg/day) for one month. Half of the rats were sacrificed at active period, the other half after a 2-week recovery period.RESULTS: The activities of serum AST, ALT, ALP, total bilirubin level were significantly higher, total protein and GSH levels were significantly reduced in the toxic control group compared to the control group. Group III had significantly higher AST and ALT activities compared to the control rats at active period, whereas after the recovery period no significant differences were observed in almost all the parameters. Moreover, no significant differences in the parameters mentioned above were observed in group IV compared to the control rats at active and recovery period.CONCLUSION: The results of this study showed that Essential forte was better as a curative agent rather than a prophylactic agent in rats.

scholarly journals Effects of Aluminium Chloride Exposure on the Histology of the Cerebral Cortex of Adult Wistar Rats

2012 ◽  
Vol 3 (1) ◽  
Author(s):  
Adebayo Adekunle Buraimoh ◽  
Samuel Adeniyi Ojo ◽  
Joseph Olajide Hambolu ◽  
Sunday Samuel Adebisi
Keyword(s):  
Cerebral Cortex ◽  
Wistar Rats ◽  
Group Iv ◽  
Aluminium Chloride ◽  
Mammalian Brain ◽  
Control Group ◽  
Perceptual Awareness ◽  
Group V ◽  
Group Iii ◽  
Group I

Aluminium (Al) is presents in many manufactured foods, medicines and is also added to drinking water for purification purposes. Human exposure to Al has been increasing over the last decades. Al exposure and neurological impairments demonstrate mixed findings. The cerebral cortex is a sheet of neural tissue that is outer-most to the cerebrum of the mammalian brain and it plays a key role in memory, attention, perceptual awareness, thought, language, and consciousness.The objectives of this study was to investigate the possible effects that aluminium Chloride could have on the histology of cerebral cortex. Total of twenty adult wistar rats were used for this experiment. The wistar rats were divided into five groups; group I was the control, group II received 475mg/Kg, group III received 950mg/kg, group IV received 1,425mg/kg and group V received 1,900mg/kg via oral intubation for a duration of Eight weeks. The wistar rats were humanly sacrificed and the brain was removed and immediately fixed in bouin fluid. The histological observations of the aluminium treated groups revealed extensive neuronal vacuolation and necrosis (neuro-degeneration) of the cerebral cortex of wistar rats.Based on our observations, we therefore conclude that Aluminium chloride exposure has neurodegenerative effects on the histology of cerebral cortex of adult wistar rats especially at higher dose. Therefore, caution should be taken in its usage.

scholarly journals Evaluation of concurrent exposure of lower concentrations of lead and endosulfan on apoptosis by scatter pattern of flow cytometry

2021 ◽  
Vol 10 (5) ◽  
pp. 294-297
Author(s):  
V Ranganathan ◽  
◽  
Jitendra Kumar Malik ◽  
GS Rao ◽  
◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Untreated Control Group ◽  
Technical Grade ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats

The effect of repeated exposure of lower doses of lead and endosulfan were evaluated on apoptosis in male wistar rats. Rats of group I served as untreated control. Group II received drinking water with lead as lead acetate @100 ppm (Pb100). Group III was given feed containing technical grade endosulfan @ 10 ppm (E10). Group IV was exposed to Pb (100) +E (10). Splenocytes were analysed for estimating apoptotic percentage in rats. The results suggest that apoptotic percentage was not changed in the lower doses of endosulfan and lead when administered alone and also in combination in the present study

scholarly journals Current role of low molecular weight heparin in the treatment of acute ischemic stroke

2017 ◽  
Vol 4 (6) ◽  
pp. 1599
Author(s):  
Karan Singh
Keyword(s):  
Ischemic Stroke ◽  
Placebo Group ◽  
Acute Ischemic Stroke ◽  
Group Iv ◽  
Control Group ◽  
High Dose ◽  
Mortality And Morbidity ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Background: Nevertheless, several studies of LMWH in acute ischemic stroke have been neutral with regard to their primary outcomes, and it remains unclear whether these drugs should be used routinely or not. Our present trial endeavors to study the efficacy of LMWH (low and high dose dalteparin) in patients with progressive ischemic stroke in terms of morbidity and mortality as compared to control group and to compare it with AS+CLOP, with respect to these event rates, at the end of treatment.Methods: Our study was performed on 38 patients of acute ischemic stroke admitted to LLR and associated Hospitals, G. S. V. M. Medical College, Kanpur and who were assigned randomly to any of the four treatment groups (0.4ml dalteparin, 0.8ml of dalteparin, O ml of placebo and aspirin +clopidogrel 150+75 mg). The standard error of proportion method and Chi square test was applied.Results: 70% of patients in group I, 62.5% in Group II and 60% Group III presented with stroke in evolution at presentation as compared to only 30% in the placebo group. 75% of patients in group I, 66.66% in group II, 50% in Group III and 33.34% in group IV had a complete recovery. 25% of patients in Group-I, 33.34% in Group-II, 50% in Group III and 66.66% in Group-IV had an incomplete recovery.Conclusions: There is no significant reduction in the mortality and morbidity amongst LMWH groups at the end of treatment and end of trial as compared to the AS+CLOP, or placebo group or even amongst the low and high dose LMWH groups.

Analysis of surface conditioning methods on core-veneer bond strength of CAD/CAM zirconia restorations

2020 ◽  
pp. 1-11
Author(s):  
Anip K. Roy ◽  
Govind N. Prasad ◽  
Tushar V. Bhagat ◽  
Saurabh Chaturvedi ◽  
Vishwanath Gurumurthy ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Surface Conditioning ◽  
Group I ◽  
Alumina Particles ◽  
Scanning Electron

BACKGROUND: The increased strength of zirconia has resulted in its widespread application in clinical dentistry. Nevertheless, the fracture of veneering porcelains remains one of the key reasons of failure. OBJECTIVE: The objective of this study was to compare and analyze the influence of surface conditioning methods on the core-veneer bond strength of zirconia restorations. METHODS: Thirty specimens of zirconia core with sizes 10 × 5 × 5 mm were layered with porcelain of sizes 5 × 3 × 3 mm. On the basis of different surface conditioning methods, four groups were made: Group I: abrasion with airborne alumina particles of 110 μm size, Group II: sandblasting with silica coated alumina particles of 50 μm in size, Group III (modified group): alteration with a coating of zirconia powder prior to sintering, and Group IV (control group): metal core specimens. The shear force of all specimens was tested using a universal testing machine with a 0.5 mm/min crosshead speed. One-way analysis of variance (ANOVA) and Tukey’s post hoc pair wise comparison (p= 0.05) were performed to analyze the shear bond strength. A scanning electron microscope was used to assess the fractured specimens. RESULTS: A statistically significant difference was noted between the groups. The mean value of shear bond strength was 40.25 MPa for Group I, 41.93 MPa for Group II, 48.08 MPa for Group III and 47.01 MPa for Group IV. CONCLUSIONS: The modified zirconia group and control group demonstrated a significantly higher mean bond strength than that of Group I, where airborne particle abrasion was used. The scanning electron microscope showed that cohesive fracture in the porcelain veneers was the main problem of failure in altered zirconia. The modified zirconia specimens in Group III demonstrated significantly improved values of shear bond strength.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

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