scholarly journals Toksisitas Partisi N-Heksan dan Etil Asetat pada Ekstrak Sargassum sp. terhadap Larva Aedes aegypti Instar III

2020 ◽  
Vol 9 (2) ◽  
pp. 143-150
Author(s):  
Alfianisa Permata Sari ◽  
Ervia Yudiati ◽  
Sunaryo Sunaryo
Keyword(s):  
Biological Activity ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Negative Control ◽  
Good Source ◽  
Body Extract ◽  
Morphological Aberration ◽  
Tlc Analysis ◽  
Dengue Epidemic

ABSTRAK: Kejadian demam berdarah dengue pada awal 2019 tercatat lebih dari 17.000 kasus demam berdarah dengan angka kematian mencapai 169 jiwa di seluruh Indonesia. Sargassum sp. merupakan jenis rumput laut cokelat berlimpah namun belum dimanffatkan dengan baik. Sargassum sp  memiliki beragam aktivitas biologis. Penelitian ini bertujuan untuk mengetahui toksisitas ekstrak rumput laut cokelat Sargassum sp. larva Aedes aegypti instar III. Ekstraksi dilakukan dengan cara maserasi sampel Sargassum sp. dengan pelarut metanol, dilanjutkan partisi dengan pelarut n-heksana dan etil asetat serta analisis fitokimia menggunakan kromatografi lapis tipis. Sepuluh ekor larva nyamuk dipaparkan dalam 100 ml aquades dengan beberapa konsentrasi ekstrak (0, 50, 100, 250, 500 dan 1000 ppm), abate sebagai kontrol positif dan DMSO dengan tiga kali ulangan. Aktivitas larvasida ekstrak Sargassum sp. juga ditentukan dengan menghitung nilai LC50 pada jam ke-72. Perubahan morfologi diamati secara mikroskopis. Fraksi n-heksana dan etil asetat Sargassum sp. memiliki nilai LC50 berturut-turut sebesar 3129.15 ppm dan 996.28 ppm. Larva yang dipaparkan dengan ekstrak etil asetat Sargassum sp. memiliki kerusakan morfologi pada bagian kepala, siphon, saluran pencernaan, papila anal serta warna tubuh menjadi lebih gelap. Keseluruhan fraksi n-heksana dan etil asetat Sargassum sp. mengandung senyawa golongan fenolat dan terpenoid. Berdasarkan hasil tersebut, fraksi etil asetat Sargassum sp. berpotensi sebagai larvasida.  ABSTRACT: In the early 2019, there has been 17,000 Indonesian people suffered and 169 died from Dengue epidemic. Sargassum sp. in Indonesian waters were plentiful, rich in biological activity and still unexpolitated. This study aimed to determine the 72-h LC50 of Sargassum sp. extract to Aedes aegypti instar III larvae. Extraction was done by maseration with methanol, partitioned with n-hexane and ethyl acetate, folowed by TLC  analysis. Ten larvae were exposed with 100 mL aqudest in a serial concentration (0; 50;100; 25; 500 dan 1000 ppm), completed with Abate® powder as positive, aquadest as negative control. All treatments were replicated three times. Observation on morphological aberration was done microscopically. 72-h LC50 of n-hexane and ethyl acetate fraction were 3129.15 ppm dan 996.28 ppm, respectively. There were larval morphologically damage in head, siphon, digestive tract and papilla anal and dark coloured body. Extract were composed with phenolate and terpeniod coumpoud. It is concluded that Sargassum sp.  extract was a good source for larvacide. 

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

2017 ◽  
Vol 13 (51) ◽  
pp. 462 ◽  
Author(s):  
Palanisamy Arulselvan ◽  
Sivapragasam Gothai ◽  
Katyakyini Muniandy ◽  
MazniAbu Zarin ◽  
TanWoan Sean ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Ethyl Acetate Fraction ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals THE BENEFITS OF ACTIVE COMPOUNDS IN KALANCHOE PINNATA (LMK) PERS ETHYL ACETATE FRACTION ON LUPUS ARTHRITIS MICE

2017 ◽  
Vol 10 (11) ◽  
pp. 199 ◽  
Author(s):  
Niken Indriyanti ◽  
Joewono Soeroso ◽  
Junaidi Khotib
Keyword(s):  
Flow Cytometry ◽  
Dendritic Cells ◽  
Ethyl Acetate ◽  
Joint Damage ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Tissue Structure ◽  
Active Compounds ◽  
Kalanchoe Pinnata

Ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers leaves (EF-KP) is a potential material to be developed for lupus treatment. The aim of this research was to measure the effects of the EF-KP on lupus arthritis mice.  The research was performed by testing of CD123+IFN-α+ dendritic cells and CD68+IL-6+ macrophages as the main biomarkers by using flow cytometry methods, and then the outcomes were directly observed in the joint’s tissue structure. The results of the research were analyzed by using statistics. The EF-KP reduces the relative percentages of CD123+IFN-α+ dendritic cells significantly (p<0.05) with the percentage of 32.95±8.25% (negative control); 23.28±9.31%* (EF-KP); 22.98±10.39%* (positive control). It also reduces the relative percentages of CD68+IL-6+ macrophages but not significantly (p>0.05). Finally, the outcome to the grade of joint damage was scored by using Pritzker method. The treated groups have one grade lower, and the joint spaces were narrower than the untreated group. The results show the ability of the active compounds in EF-KP, which are comparable to 0.042 mg/Kg quercetin, to inhibit the progress of lupus arthritis pathogenesis in mice. It reveals the effectiveness of the EF-KP in human with lupus arthritis. However, the further clinical research is necessary.

scholarly journals Efek Larvasida Fraksi Etil Asetat Daun Bangun-bangun (Plectranthus amboinicus) terhadap Mortalitas Larva Aedes aegypti

2020 ◽  
Vol 12 (1) ◽  
pp. 27-36
Author(s):  
Sogandi Sogandi ◽  
Fadhli Gunarto
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Acid Group ◽  
Chemical Substance ◽  
Probit Analysis ◽  
Dengue Haemorrhagic Fever ◽  
Haemorrhagic Fever ◽  
Extract Concentration ◽  
Plectranthus Amboinicus

Abstract. Mosquitoes are insects that can transmit diseases to humans through their bites. Dengue haemorrhagic fever is one of the diseases that could be transmitted by Aedes aegypti mosquito. The use of insecticides to control the occurrence of dengue haemorrhagic fever builds resistance of the mosquito towards the chemical substance. Therefore, natural larvicide was used as an alternative to chemical insecticides. This research was conducted to study the effectiveness of larvicides from the ethyl acetate fraction of Bangun-bangun leaves (Plectranthus amboinicus) in killing the Aedes aegypti larvae and to identify the active larvicide compounds. This laboratory experiment research tested six extract concentrations (0.0; 1.0; 1.8; 3.2; 5.6; 10.0%) in three replications. Observations were administered for 48 hours to count the number of the dead Aedes aegypti instar III larvae obtained from the Entomology Laboratory of the Faculty of Veterinary, IPB. The results showed that the larvae mortality increased as the extract concentration increased. The percentage of Aedes aegypti killed reached 96% at an extract concentration of 10%. Meanwhile, the probit analysis showed that LC50 was found at a concentration of 5.56%. The identification of active compounds using GCMS revealed that the larvicidal compounds in ethyl acetate fraction were the ones from an organic acid group which were, stearic acid, and palmitic acid.  Keywords: Aedes aegypti, identification, larvacidal, mortality   Abstrak. Nyamuk merupakan serangga yang dapat menularkan penyakit melalui gigitannya. Salah satu penyakit yang ditularkan adalah demam berdarah dengue yang dibawa oleh nyamuk Aedes aegypti. Pengendalian demam berdarah dengue menggunakan insektisida berbahan kimia dapat menyebabkan resisten nyamuk terhadap insektisida meningkat, oleh karena itu larvasida alami diharapkan dapat menjadi alternatif pengganti insektisida kimia. Tujuan dari penelitian ini adalah mengetahui afektifitas larvasida dari fraksi etil asetat daun Bangun-bangun (Plectranthus amboinicus) terhadap mortalitas larva Aedes aegypti dan mengidentifikasi jenis senyawa aktif yang berperan sebagai larvasida. Penelitian ini merupakan eksperimen laboratorium dengan enam konsentrasi ekstrak (0,0; 1,0; 1,8; 3,2; 5,6; 10,0%) dan tiga kali ulangan. Pengamatan dilakukan selama 48 jam terhadap jumlah kematian larva Aedes aegypti instar III yang diperoleh dari Laboratorium Entomologi Fakultas Kedokteran Hewan, IPB. Hasil penelitian menunjukkan bahwa kematian larva meningkat seiring dengan meningkatnya konsentrasi ekstrak. Kematian Aedes aegypti mencapai 96% pada konsentrasi ekstrak 10% dan hasil analisa probit menunjukkan LC50 terdapat pada konsentrasi 5,56%.  Identifikasi senyawa aktif menggunakan GCMS menunjukkan bahwa senyawa yang bersifat larvasida dalam fraksi etil asetat ini adalah senyawa dari golongan asam organik yaitu, asam stearate, dan asam palmitat.  Kata Kunci: Aedes aegypti, identifikasi, larvasida, mortalitas

scholarly journals PENDAYAGUNAAN DUA JENIS ZINGIBERACEAE [C. mangga (temu mangga) dan K. angustifolia (kunci menir)] SEBAGAI SUMBER BAHAN IMUNOMODULATOR SECARA IN VITRO

2016 ◽  
Vol 11 (3) ◽  
pp. 435
Author(s):  
Praptiwi . ◽  
Chairul .
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Phyllanthus Niruri ◽  
Vitro Assay ◽  
Macrophage Cells ◽  
Almost All

Observation of imunomodulation properties of methanol extract derive (hexane,ethyl acetate, methanol/water) of Curcuma mangga (temu mangga) and Kaempferia angustifolia (kunci menir) had been carried out by in vitro assay. It was done by determining the phagocytised activity and capacity of macrophage cells of mice (Mus musculus) peritonium inducted with Staphyllococcus epidermidis. The result showed that each fraction had different phagocytised activity and capacity. Almost all of the fractions tested improved the activity (50–96 %) and capacity macrophage cells phagocytised compared to negative control (52 dan 502) and positive control (97 dan 1076). The positive control was 1000 ug Stimuno (Phyllanthus niruri extract), while the negative control was aquadest. The ethyl acetate fraction of Z. cassumunar (bangle) had highestphagocytised activity and capacity followed by C. mangga (temu mangga) and K. Angustifolia (kunci menir). It can be concluded that there were significant differences of phagocytised activity and capacity among fraction tested.Keywords : Zingiberaceae, Curcuma mangga, Kaempferia rotunda, Zingibercassumunar, immunomodulator, phagocytised, macrophage cells.

scholarly journals IN VITRO STUDY OF THE BRONCHORELAXATION EFFECT OF COPTOSAPELTA FLAVESCENS KORTH. ROOT’S EXTRACT AND FRACTIONS

2019 ◽  
pp. 94-97
Author(s):  
KHEMASILI KOSALA ◽  
MOCH. ARIS WIDODO ◽  
SANARTO SANTOSO ◽  
SETYAWATI SOEHARTO
Keyword(s):  
Guinea Pig ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control ◽  
Hexane Fraction ◽  
Organ Bath ◽  
Response Curves ◽  
Methanol Fraction

Objective: This study aimed to compare the bronchorelaxation effect of the methanol extract, hexane fraction, ethyl acetate fraction, and methanol fraction of Coptosapelta flavescens Korth. roots (CFRs) on the isolated bronchial rings of guinea pig. Methods: Research design was experimental in which a 4-mm long bronchus of a male guinea pig was kept in an isolated organ bath containing Krebs-Henseleit solution at 37°C, pH 7.4, and flooded with carbogen gas. The bronchial ring was precontracted with methacholine and then given six doses of C. flavescens Korth. root’s methanol extract (MECFR) solution cumulatively. The extract’s solvent 10% dimethyl sulfoxide ethanol was used as negative control. This procedure was then repeated using CFRs hexane fraction (HFCFR) solution, CFRs ethyl acetate fraction (EAFCFR), and CFRs methanol fraction (MFCFR). Results: The bronchorelaxation effect of MECFR, MFCFR, EAFCFR, and HFCFR is shown by their dose-response curves (DRCs) which are significantly different compared with the extracts solvent’s DRC. The maximal efficacy (Emax) of MFCFR was the same (p>0.05) as the MECFRs Emax, but the EAFCFRs and HFCFRs Emax were smaller (p<0.05) than the MECFRs Emax. Conclusion: The bronchorelaxation effect of the MECFR on the guinea pig’s bronchial ring is similar to the CFRs methanol fraction, and is stronger than the CFRs ethyl acetate fraction and CFRs hexane fraction.

scholarly journals Uji antioksidan dan bioaktivitas fraksi etil asetat batang jarak tintir (Jatropha multifia L.)

2020 ◽  
Vol 9 (1) ◽  
pp. 64
Author(s):  
Akhirul Kahfi Syam ◽  
Julia Ratnawati ◽  
Desti Hermawati ◽  
Puspita Satriyani Permatasari Surasa
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Brine Shrimp Lethality ◽  
Soxhlet Apparatus ◽  
Long Time ◽  
Dpph Scavenging ◽  
Tlc Analysis ◽  
Jatropha Multifida

Abstrak Latar Belakang: Jatropha multifida L. atau jarak tintir merupakan tanaman yang digunakan secara empiris getahnya sebagai obat luka oleh masyarakat Indonesia sejak lama. Penelitian ini merupakan lanjutan dari penelitian sebelumnya mengenai batang jarak tintir yang telah diuji toksisitas dengan metode BSLT dan peredaman antioksidan dengan metode DPPH yang secara berturut memberikan nilai LC50 terendah 3,69 µg/mL dan IC50 56,85 µg/mL pada ekstrak etil asetat. Metode: Penelitian meliputi tahap-tahap pengumpulan bahan uji batang jarak tintir, penyiapan simplisia, pembuatan ekstrak dengan 3 pelarut yang berbeda kepolarannya penapisan fitokimia dari simplisia, fraksinasi menggunakan metode Kromatografi Cair Vakum (KCV) dari ekstrak etil asetat menjadi 4 fraksi. Hasil: Hasil pengujian antioksidan metode peredaman DPPH memberikan IC50 terhadap f1 (n-heksan 75%:etil asetat 25%), f2 (n-heksan 50%:etil asetat 50%), f3 (n-heksan 25%:etil asetat 75%), dan f4 (etil asetat 100%) secara berturut-turut adalah 3759,79 µg/mL; 790,94 µg/mL; 167,41 µg/mL; dan 164,82 µg/mL. Hasil pengujian BSLT terhadap fraksi etil asetat memberikan hasil LC50 sebesar f1 64,43 µg/mL; f2 37,54 µg/mL; f3 41,94 µg/mL; dan f4 25,70 µg/mL. Kesimpulan: F4 merupakan fraksi dengan nilai IC50 dan LC50 yang paling rendah dari seluruh fraksi, yang menunjukkan f4 merupakan fraksi yang paling aktif sebagai antioksidan dan BSLT serta diduga golongan flavonoid yang diketahui dari profil kromatogram KLT dengan Rf 0,57 yang memberikan hasil positif terhadap penampak bercak FeCl3 dan AlCl3 merupakan senyawa aktif dari kedua aktifitas tersebut. Kata kunci: Antioksidan, Brine Shrimp Lethality Test, DPPH, Jatropha multifida L.   ANTIOXIDANT AND BIOACTIVITY ASSAYS OF ETHYL ACETATE FRACTION OF JARAK TINTIR STEM (Jatropha multifida L) Abstract Background: Jatropha multifida L. or jarak tintir was empirically used as wound treatment for the sap by Indonesian people for a long time ago. The toxicity and antioxidant capacity of the jarak tintir stem was done by brain shrimp lethality test (BSLT) with LC50 3,69 µg/mL and DPPH scavenging activity with IC50 56,85 µg/mL for ethyl acetate extract. Method: Extraction was  performed  by  continuous  extraction  with Soxhlet  apparatus  using  various polarity  solvents  (n-hexane, ethyl acetate, and methanol) and ethyl acetate extract was fractioned by vacuum liquid chromatography (VLC) into 4 fraction with gradien eluen of n-hexane and ethyl acetate. Results: Antioxidant assay gave IC50 for f1 (n-hexane 75%:ethyl acetate 25%), f2 (n-hexane 50%: ethyl acetate 50%), f3 (n-hexane 25%: ethyl acetate 75%), and f4 (ethyl acetate 100%) respectively as 3759,79 µg/mL; 790,94 µg/mL; 167,41 µg/mL; and 164,82 µg/mL. BSLT assays for each fractions gave LC50 as f1 64,43 µg/mL; f2 37,54 µg/mL; f3 41,94 µg/mL; and  f4 25,70 µg/mL. Conclusion: Fraction 4 was the best fraction with the lowest IC50 and LC50 with flavonoid was suspected as responsible compound for both assays after TLC analysis that gave spot with Rf 0.57 and gave positive result after sprayed with FeCl3 dan AlCl3 spray reagent. Keywords: Antioxidant, Brine Shrimp Lethality Test, DPPH, Jatropha multifida L

scholarly journals ANREDERA CORDIFOLIA LEAVES FRACTION AS AN ANTI-HYPERLIPIDEMIA

2016 ◽  
Vol 9 (6) ◽  
pp. 82 ◽  
Author(s):  
Dwintha Lestari
Keyword(s):  
Ethyl Acetate ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Water Fraction

Objective: The objective of this research was to evaluate reduction of blood cholesterol level by fraction extracts of binahong (Anredera cordifolia) on male Wistar rats. Methods: The rats were given high fat food every day for 3 months and pure cholesterol on week 0, 3, 7 and 11.  After that each binahong fraction (n-hexane fraction, ethyl acetate fraction and water fraction) with doses of 50 mg/kg bw, 100 mg/kg bw, 200 mg/kg bw were given orally for 3 weeks and observed for lipid profile on day 21.   Results: The result showed that n-hexane, ethyl acetate and water fractions of binahong leaves decreased total cholesterol, triglyceride and LDL level, and did not influence the HDL level. Meanwhile statin decreased total cholesterol, triglyceride and LDL level, and increased HDL level. Conclusion: Ethyl acetate fraction of binahong leaves significantly reduced total cholesterol level, triglycerides, LDL level compared to negative control group (p< 0.05) and had no influence in HDL level. Keywords: Atherosclerosis, Anredera cordifolia, cholesterol

scholarly journals Pengembangan Biolarvasida Jentik Nyamuk Aedes aegypti Berbahan Aktif Ekstrak Beluntas (Pluchea indica Less.)

REAKTOR ◽  
2017 ◽  
Vol 16 (3) ◽  
pp. 103
Author(s):  
Agus Rochmat ◽  
Mitha Fuji Adiati ◽  
Zahrotul Bahiyah
Keyword(s):  
Acetic Acid ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Quinic Acid ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Mosquito Larvae ◽  
Natural Phenomena ◽  
Leaf Extracts

Abstract POTENSIAL DEVELOPMENT OF EXTRACT BELUNTAS (Plucea indica Less.) as BIOLARVACIDE TO MOSQUITO Aedes aegypti LARVAE. The eradication of Aedes aegyptY mosquito is difficult because they have the ability to adapt the environment which makes it very tough. Although, there are not disturbances due to natural phenomena or human intervention. Termination of the mosquito life cycle is an alternative to reduce the mosquito population. The antimicrobial of beluntas extract is expected to have the ability biolarvacide on mosquito larvae. The biolarvasicide of beluntas leaf extracts was determined LC50 values and strengthened by identification of the active compound. The biolarvacide tested was conducted on the larvae of Aedes aegypti with variations extract concentrations of 50, 100, 250, 500 and 1000 ppm for 24 hours observation. The experimental results found that yield of ethanol extract, extract n-hexane and ethyl acetate extract: 3.8742%, 1.2054% and 1.8627%. While the value of LC50 to extract n-hexane and ethyl acetate respectively amount to 46.09 ppm and 108.79 ppm. LC50 value obtained belong biolarvacide active and positive control using abate value LC100 Abate at a concentration of 100 ppm. The ability biolarvacide ethyl acetate fraction only make the mosquito larvae die, anwhile the fraction of n-hexane can degrade the cells larvae destroyed. The ability biolarvacide beluntas extract was corroborated by the results of GC-MS analysis which showed contains active compounds beluntas such as quinic acid, hydrazinecarboxamide, benzene acetic acid, and 1,2-benzendicarboxylic acid which is a compound of larvicides. Keywords: biolarvacide; beluntas; LC50, GC-MS  Abstrak Pemberantasan nyamuk Aedes aegypti sulit dilakukan karena mereka memiliki kemampuan adaptasi lingkungan yang membuat sangat tangguh, meski ada gangguan  akibat  fenomena  alam ataupun  intervensi manusia. Pemutusan siklus hidup nyamuk merupakan alternative dalam mengurangi populasi nyamuk. Sifat antimikroba ekstrak nyamuk diharapkan dapat memiliki kemampuan biolarvasida pada jentik nyamuk. Kemampuan biolarvasida ekstrak daun beluntas ditentukan melalui nilai LC50 dan diperkuat dengan identifikasi kandungan senyawa aktif. Uji biolarvasida ini dilakukan terhadap larva nyamuk Aedes aegypti dengan variasi konsentrasi ekstrak 50, 100, 250, 500 dan 1000 ppm selama 24 jam pengamatan. Hasil percobaan diketahui bahwa: rendemen untuk ekstrak etanol, ekstrak n-heksana dan ekstrak etil asetat masing-masing sebesar 3,8742 %, 1,2054 % dan 1,8627 % sementara nilai LC50 untuk ekstrak n-heksan dan etil asetat masing-masing sebesar 46,09 ppm dan 108,79 ppm. Nilai LC50 yang diperoleh termasuk golongan biolarvasida aktif dan kontrol positif menggunakan abate memiliki nilai LC100 Abate pada konsentrasi 100 ppm. Kemampuan biolarvasida fraksi etil asetat hanya membuat larva nyamuk mati sementara fraksi n-heksana dapat mendegradasi sel larva hingga hancur. Kemampuan biolarvasida aktif ekstrak beluntas ini dikuatkan dengan hasil analisa GC-MS yang menunjukkan bahwa kandungan senyawa aktif ekstrak daun beluntas seperti quinic acid, hydrazinecarboxamide, benzene acetic acid, dan 1,2-benzendicarboxylic acid yang merupakan senyawa larvasida.

scholarly journals BIOLOGICAL ACTIVITY OF EXTRACTS OF PLANT HAPLOPHYLLUM PERFORATUM

2020 ◽  
Vol 2 (10(79)) ◽  
pp. 76-79
Author(s):  
U. Mamarozikov ◽  
S. Turaeva ◽  
R. Zakirova ◽  
N. Khidirova
Keyword(s):  
Biological Activity ◽  
Ethyl Acetate ◽  
High Activity ◽  
Fungicidal Activity ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Chloroform Fraction ◽  
Microscopic Fungus ◽  
Haplophyllum Perforatum ◽  
Water Alcohol

The article presents the results of studies of the fungicidal and growth-stimulating activity of various parts of the ethanol extract of the plant H. perforatum. As a result of fractionation of the extract, a hexane fraction, a chloroform fraction, an ethyl acetate fraction, and a water-alcohol residue were obtained. The ethanol extract showed a high fungicidal activity against the microscopic fungus Fusarium oxisporum. The study of the growthstimulating effect on the culture of cucumbers showed a high activity of the hexane part of the extract.

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