scholarly journals Efek Larvasida Fraksi Etil Asetat Daun Bangun-bangun (Plectranthus amboinicus) terhadap Mortalitas Larva Aedes aegypti

2020 ◽  
Vol 12 (1) ◽  
pp. 27-36
Author(s):  
Sogandi Sogandi ◽  
Fadhli Gunarto
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Acid Group ◽  
Chemical Substance ◽  
Probit Analysis ◽  
Dengue Haemorrhagic Fever ◽  
Haemorrhagic Fever ◽  
Extract Concentration ◽  
Plectranthus Amboinicus

Abstract. Mosquitoes are insects that can transmit diseases to humans through their bites. Dengue haemorrhagic fever is one of the diseases that could be transmitted by Aedes aegypti mosquito. The use of insecticides to control the occurrence of dengue haemorrhagic fever builds resistance of the mosquito towards the chemical substance. Therefore, natural larvicide was used as an alternative to chemical insecticides. This research was conducted to study the effectiveness of larvicides from the ethyl acetate fraction of Bangun-bangun leaves (Plectranthus amboinicus) in killing the Aedes aegypti larvae and to identify the active larvicide compounds. This laboratory experiment research tested six extract concentrations (0.0; 1.0; 1.8; 3.2; 5.6; 10.0%) in three replications. Observations were administered for 48 hours to count the number of the dead Aedes aegypti instar III larvae obtained from the Entomology Laboratory of the Faculty of Veterinary, IPB. The results showed that the larvae mortality increased as the extract concentration increased. The percentage of Aedes aegypti killed reached 96% at an extract concentration of 10%. Meanwhile, the probit analysis showed that LC50 was found at a concentration of 5.56%. The identification of active compounds using GCMS revealed that the larvicidal compounds in ethyl acetate fraction were the ones from an organic acid group which were, stearic acid, and palmitic acid.  Keywords: Aedes aegypti, identification, larvacidal, mortality   Abstrak. Nyamuk merupakan serangga yang dapat menularkan penyakit melalui gigitannya. Salah satu penyakit yang ditularkan adalah demam berdarah dengue yang dibawa oleh nyamuk Aedes aegypti. Pengendalian demam berdarah dengue menggunakan insektisida berbahan kimia dapat menyebabkan resisten nyamuk terhadap insektisida meningkat, oleh karena itu larvasida alami diharapkan dapat menjadi alternatif pengganti insektisida kimia. Tujuan dari penelitian ini adalah mengetahui afektifitas larvasida dari fraksi etil asetat daun Bangun-bangun (Plectranthus amboinicus) terhadap mortalitas larva Aedes aegypti dan mengidentifikasi jenis senyawa aktif yang berperan sebagai larvasida. Penelitian ini merupakan eksperimen laboratorium dengan enam konsentrasi ekstrak (0,0; 1,0; 1,8; 3,2; 5,6; 10,0%) dan tiga kali ulangan. Pengamatan dilakukan selama 48 jam terhadap jumlah kematian larva Aedes aegypti instar III yang diperoleh dari Laboratorium Entomologi Fakultas Kedokteran Hewan, IPB. Hasil penelitian menunjukkan bahwa kematian larva meningkat seiring dengan meningkatnya konsentrasi ekstrak. Kematian Aedes aegypti mencapai 96% pada konsentrasi ekstrak 10% dan hasil analisa probit menunjukkan LC50 terdapat pada konsentrasi 5,56%.  Identifikasi senyawa aktif menggunakan GCMS menunjukkan bahwa senyawa yang bersifat larvasida dalam fraksi etil asetat ini adalah senyawa dari golongan asam organik yaitu, asam stearate, dan asam palmitat.  Kata Kunci: Aedes aegypti, identifikasi, larvasida, mortalitas

Potency of Ethanol Extract and Fractions of the Leaf of Annona muricata against Aedes aegypti Larvae

2020 ◽  
Vol 41 (2) ◽  
Author(s):  
I.A Umohata ◽  
P.M.E Ubulom ◽  
P.S Thomas ◽  
U.S Nwangwu
Keyword(s):  
Aedes Aegypti ◽  
Ethanol Extract ◽  
Exposure Period ◽  
Ethyl Acetate Fraction ◽  
Probit Analysis ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Plant Metabolites ◽  
Larval Habitats ◽  
Extract Concentration

Annona muricata L. is a plant commonly used in Southern Nigeria, for nutritional and medicinal purposes. This study wascarried out to determine the larvicidal potentials of ethanol extract and fractions of the leaf of A. muricata. The ethanol leafextract was subjected to phytochemical screening using standard protocol. The leaf crude extract and fractions (i.e., ethylacetate and n-hexane) were used for larvicidal assay. The stock solution (5g each in 100ml of water) was prepared. From thestock solution 0.15, 0.30, 0.45, 0.60 and 0.75_ %w/v concentrations of extract and fractions were prepared and eachconcentration of the extract and fractions had 5 replicates. The control (100ml water and larval nutrient only) was alsoreplicated . Twenty (20), third instar, larvae of Aedes aegypti were exposed to each extract concentration and fractions andtheir controls. Larval nutrient was added to each experimental set up. Observations were made after 24 and 48 hours exposureperiod. Phytochemical screening revealed the presence of some plant metabolites (alkaloids, saponins, tannins etc).Mortality of larvae exposed to the extract and fractions increased with increased in extract concentration and exposure time.This study revealed a differential potency of the extract and fractions used and a difference in susceptibility of larvae to theextract and fractions as evident by the 48hLC values obtained. Exposure of larvae to 0.75_ %w/v of ethanol extract and ethyl 50acetate fraction resulted in 100 _% mortality after 48 hours exposure period. Probit analysis gave 48hr_LC50 values of 0.33and 0.24_ %w/v for ethanol extract and ethyl acetate fraction, respectively. The n-hexane fraction was the least potent with48h_LC50 value of 0.5461%w/v. Results obtained from this study suggest that the leaf extract and fractions of A. muricatahold potential as larvicides against Ae. aegypti. Its active ingredient should be isolated, characterized and formulated for usein larval habitats. Keywords: Potency, Extract, Fractions, Annona muricata, Aedes aegypti

scholarly journals Aktivitas larvasida fraksi metanol dan etil asetat buah lakum (Cayratia trifolia (L.) Domin.) terhadap larva nyamuk Aedes aegypti

2021 ◽  
Vol 10 (1) ◽  
pp. 1-13
Author(s):  
Diah Wulandari Rousdy ◽  
Elvi Rusmiyanto Pancaning Wardoyo ◽  
Siti Ifadatin
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Hemorrhagic Fever ◽  
Ethyl Acetate Fraction ◽  
Probit Analysis ◽  
Mortality Data ◽  
Phytochemical Analysis ◽  
Methanol Fraction ◽  
Lc50 Value ◽  
Cayratia Trifolia

ABSTRAKAedes aegypti merupakan serangga vektor dari virus Dengue penyebab Demam Berdarah Dengue (DBD). Kandungan senyawa dalam tumbuhan lakum berpotensi sebagai larvasida. Buah lakum dimaserasi dengan pelarut methanol kemudian difraksinasi menggunakan etil asetat. Fraksi diteliti kandungan senyawanya menggunakan analisis fitokimia. Uji larvasida diamati selama 48 jam terdiri dari tujuh konsentrasi berseri yaitu kontrol; 0,04; 0,12; 0,2; 0,6; 1; 3% (b/v). Setiap konsentrasi diulang 3 kali dan setiap ulangan terdiri dari 10 ekor larva nyamuk Ae. aegypti. Data mortalitas dianalisis menggunakan analisis probit untuk menentukan nilai LC50. Fraksi metanol buah lakum  berpotensi sebagai larvasida dengan nilai LC50 6 jam sebesar 1,125%. Fraksi etil asetat  buah lakum  berpotensi sebagai larvasida dengan nilai LC50 6 jam sebesar 1,102%. Buah lakum mengandung senyawa: polifenol, kuinon, flavonoid, alkaloid dan terpenoid. Kata kunci: Aedes aegypti; Cayratia trifolia (L.) Domin; larvasida; LC50  ABSTRACTLarvacidal activity of methanol and ethyl acetate fraction of lakum fruits (Cayratia trifolia (L.) Domin.) against Aedes aegypti larvae. Aedes aegypti is a vector from the Dengue virus which causes Dengue Hemorrhagic Fever (DHF). The compounds of lakum plants has the potential larvicide. The fruit of C. trifolia was macerated with methanol then fractionated using ethyl acetate as a solvent. The fraction was examined for its compound using phytochemical analysis. Larvacide test was observed for 48 hours consisting of seven concentration of 0 (control); 0.04; 0.12; 0.2; 0.6; 1; 3% (w/v). Each concentration was repeated 3 times and each repetition consisted of 10 larvae of Ae. aegypti. Mortality data were analyzed using probit analysis to determine the LC50 of the fraction of methanol and ethyl acetate from lakum fruit. The methanol fraction has the potential larvicide with a six hour LC50 value 1.125%. The ethyl acetate fraction has the potential larvicide with a six hour LC50 value 1.102%. The C. trifolia fruits contains polyphenols, quinones, flavonoids, alkaloids and terpenoids. Keywords: Aedes aegypti; Cayratia trifolia (L.) Domin.; larvacide, LC50

scholarly journals AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
I Nyoman Pugeg Aryantha ◽  
Wahyu Setyaji Dwiantara
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Beauveria Bassiana ◽  
Larvicidal Activity ◽  
Control Agent ◽  
Probit Analysis ◽  
Hexane Extract ◽  
Potato Dextrose Broth ◽  
Synthetic Pesticides ◽  
Biological Insect Control

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.   

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals Faktor-Faktor yang Berhubungan dengan Upaya Pencegahan Demam Berdarah Dengue (DBD) Menggunakan Prinsip Menguras, Menutup, dan Memanfaatkan Kembali (3M)

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Dian Emilia Sari
Keyword(s):  
Aedes Aegypti ◽  
Dengue Haemorrhagic Fever ◽  
P Value ◽  
Haemorrhagic Fever ◽  
Chi Square ◽  
Cross Sectional

Dengue Haemorrhagic Fever (DHF) atau Demam Berdarah Dengue adalah penyakit menular yang disebabkan oleh virus dengue yang ditularkan melalui gigitan nyamuk Aedes Aegypti. Keluarga dapat berperan secara aktif dalam melakukan gerakan PSN dengan cara 3M Plus, yaitu menguras, menutup dan memanfaatkan kembali. Penilitian ini bertujuan untuk diketahuinya faktor-faktor yang berhubungan dengan upaya pencegahan DBD di Puskesmas 4 Ulu Palembang Tahun 2019. Penelitian ini menggunakan desain kuantitatif dengan pendekatan cross sectional. Sampel dalam penelitian ini adalah  pengunjung Puskesmas berjumlah 56 orang yang dipilih  menggunakan tekhnik accidental sampling. Pengumpulan data menggunakan  kuesioner. Analisis hubungan dengan menggunakan uji Chi Square (α = 0.05). Penelitian ini dilaksanakan pada tanggal 1-5 Juli 2019. Hasil analisis didapatkan nilai  pengetahuan (p value = 0.006); sikap (p value = 0.000); tingkat pendidikan (p value = 0.000). Disimpulkan adanya hubungan antara pengetahuan,sikap, dan tingkat pendidikan dengan upaya pencegahan DBD. Diharapkan terhadap masyarakat agar lebih aktif lagi bertindak dalam pencegahan DBD, seperti melakukan gerakan pemberantasan sarang nyamuk 3M Plus.

The Dynamics of an Adult Population of Aedes aegypti in Relation to Dengue Haemorrhagic Fever in Bangkok

10.2307/3042 ◽  
1969 ◽  
Vol 38 (3) ◽  
pp. 661 ◽  
Author(s):  
P. M. Sheppard ◽  
W. W. Macdonald ◽  
R. J. Tonn ◽  
B. Grab
Keyword(s):  
Aedes Aegypti ◽  
Adult Population ◽  
Dengue Haemorrhagic Fever ◽  
Haemorrhagic Fever

scholarly journals DAYA BUNUH EKSTRAK DAUN TOMAT (Solanum lycopersicum L.) TERHADAP LARVA NYAMUK Aedes aegypti

2018 ◽  
Vol 2 (1) ◽  
pp. 36
Author(s):  
Setyo Dwi Santoso ◽  
Abdul Chamid ◽  
Dwi Viddi Kusuma Pratiwi
Keyword(s):  
Aedes Aegypti ◽  
Solanum Lycopersicum ◽  
Leaf Extract ◽  
Chemical Compounds ◽  
Hemorrhagic Fever ◽  
Probit Analysis ◽  
Tomato Leaf ◽  
Mosquito Larvae ◽  
Extract Concentration ◽  
Solanum Lycopersicum L

Dengue hemorrhagic fever (DHF) is a very dangerous disease, caused by Aedes aegypti mosquitoes. One alternative to controlling Aedes aegypti mosquitoes is to use insecticides. But it would be much better if the insecticides used are natural and environmentally friendly, one of them by using Tomato Leaf Extract (Solanum lycopersicum l.). Tomato leaves contain chemical compounds class Alkaloids and Saponins and can serve as anti larvacide. This research is a laboratory experiment which aims to find out the effect of tomato leaf extract (Solanum lycopersicum L.) activity on the death of III instar larvae of Aedes aegypti mosquito. the effectiveness of samples on Aedes aegypti mosquito larvae by treatment of variation of extract concentration 0.2%, 0.4%, 0.6%, 0.8%, 1% with repetition 2 times. In the results of research showed that the smallest concentration that can kill Aedes aegypti mosquito larvae is 0.6% at 16 hours and probit analysis showed that significance is 0,608 greater than intercept 0.003, it shows that the extract of tomato leaves affects the death of III instar larvae of Aedes aegypti mosquito.

scholarly journals Screening of Different Extracts of Marine Macro Green Algae for Larvicidal Activity against Dengue Fever Mosquito, Aedes aegypti (Diptera: Culiadae)

2017 ◽  
Vol 62 ◽  
pp. 44-51
Author(s):  
G. Adaikala Raj ◽  
M. Jayaraman ◽  
S. Krishnamoorthy ◽  
M. Chandrasekaran ◽  
V. Venkatesalu
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Green Algae ◽  
Larvicidal Activity ◽  
Ethyl Acetate Extract ◽  
Larval Mortality ◽  
Control Agent ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Ethyl Acetate Extracts

The present study larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol extracts of Halimeda macroloba, Decsne, Caulerpa racemosa (Frosk) Weber-Van-Bosses and Ulva lactuca Lin, (Chlorophyceae) against Aedes aegypti. The marine macro green algae extracts were tested against early 4th instar larvae of A. aegypti using WHO protocol and concentrations of 200, 400, 600, 800 and 1000 ppm. The observed mortality was made 24 and 48 h after treatment, data was subjected to probit analysis to determine the lethal concentration (LC50 and LC90) of the treated larvae of the tested species.Among the tested extracts the maximum efficicacy was observed in the ethyl acetate extracts. The ethyl acetate extracts of the seaweeds showed the presence of terpenoids, tannins and phenolic compounds stronger than the other extracts. The results revealed that all the extracts showed varied levels of larvicidal activity against A. aegypti tested. However, the ethyl acetate extract of C. racemosa showed remarkable larvicidal activity against A. aegypti (LC50=579.9 and LC90=1255.4 ppm values at 24 h and LC50 =495.4 and LC90 =1073.9 ppm at 48 h) followed by U. lactuca (LC50=588.1 and LC90=1290.7 ppm values at 24 h, and LC50= 530.8 and LC90= 1160.0 ppm at 48 h), respectively. The lowest larval mortality was observed with hexane extract of H. macroloba against A. aegypti with values of LC50=1116.8 and LC90= 1824.5 ppm (after 24 h) and LC50=1059.9 and LC90=1768.3 ppm (after 24 h). The present studies indicate that the larvicidal activity and phytochemicals derived from the ethyl acetate extract of C. racemosa have the potential to be used as an ideal eco-friendly approach and effective mosquito vector control agent.

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