scholarly journals CC and CXC Chemokines Patterns in Psoriasis Determined by Protein Array Method Were Influenced by Goeckerman’s Therapy

2009 ◽  
Vol 52 (1) ◽  
pp. 9-13 ◽  
Author(s):  
David Pohl ◽  
Ctirad Andrýs ◽  
Lenka Borská ◽  
Zdeněk Fiala ◽  
Květa Hamáková ◽  
...  
Keyword(s):  
Blood Donors ◽  
Uv Light ◽  
Coal Tar ◽  
Serum Levels ◽  
Protein Array ◽  
Control Group ◽  
Healthy Children ◽  
Subsequent Exposure ◽  
Pasi Score ◽  
Daily Application

Goeckerman’s therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. The aim of this study was to evaluate the influence of Goeckerman’s therapy of psoriasis on the levels of proangiogenic chemokines ENA-78 (CXCL5, Epithelial Cell Derived Neutrophil Attractant- 78), GRO alpha (CXCL1, Growth-Related Oncogene), IL-8 (CXCL8, Interleukin-8), MCP-1 (CCL2, Monocyte Chemotactic (Chemoattractant) Protein 1) and RANTES (CCL5, Regulated on Activation of Normal T Cell Expressed and Secreted) in peripheral blood of 22 children’s patients with psoriasis. 22 otherwise healthy children serve as a control group. The serum levels of chemokines were determined by commercial membrane protein array technique (RayBiotech, USA). Efficacy of Goeckerman’s therapy was delineated by PASI score. Disease activity was significantly diminished by Goeckerman’s therapy (p<0.001). Serum levels of GRO alpha and MCP-1 in patients before GT were significantly higher than those measured in healthy blood donors (GRO alpha: p=0.0128 and MCP-1: p=0.0003). Serum levels of GRO alpha, MCP-1 and RANTES were significantly diminished by GT (GRO alpha: p=0.002, MCP-1: p=0.048 and RANTES: p=0.0131). Compared to the healthy controls, serum level of MCP-1 remained significantly increased in psoriasis patients after GT (p<0.0001). In conclusion, we found that the GT of psoriasis influenced the serum levels of proinflammatory and proangiogenic chemokines, especially GRO alpha, MCP-1 and RANTES. It could be the cause for decreased proangiogenic activity which is described after GT of psoriasis.

scholarly journals Serum Level of a Soluble Form of Endoglin (CD105) is Decreased after Goeckerman’s Therapy of Psoriasis

2011 ◽  
Vol 54 (2) ◽  
pp. 59-62 ◽  
Author(s):  
David Pohl ◽  
Ctirad Andrýs ◽  
Lenka Borská ◽  
Zdeněk Fiala ◽  
Květa Hamáková ◽  
...  
Keyword(s):  
Serum Level ◽  
Blood Donors ◽  
Uv Light ◽  
Coal Tar ◽  
Severity Index ◽  
Serum Levels ◽  
Soluble Form ◽  
Control Group ◽  
Angiogenic Activity ◽  
Pasi Score

Background. Goeckerman’s therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Disturbances in angiogenic activity are characteristic for the immunopathogenesis of psoriasis. The aim of study was to evaluate the influence of GT of psoriasis on proinflammatory and angiogenic activities expressed as changes in levels of endoglin (CD105). Methods. Serum levels of a soluble form of endoglin were measured in peripheral blood samples of 38 patients with psoriasis before and after therapy. Sixty three otherwise healthy blood donors serve as a control group. The efficacy of GT was expressed as changes in Psoriasis Area and Severity Index (PASI). Results. PASI score was significantly diminished by GT (p<0.001). Serum levels of soluble CD105 were significantly diminished after GT. The serum level of soluble CD105 dropped from 7.85 ± 2.26 ng/ml before therapy to 7.01 ± 1.71 ng/ml after therapy (p= 0.0002). Compared to serum levels of soluble CD105 in healthy blood donors, serum levels of soluble CD105 in patients before GT were significantly higher (p<0.001) and remained elevated after therapy (p<0.001). Angiogenic activity expressed as serum endoglin is diminished in patients with psoriasis treated by GT.

scholarly journals Features of immune response in children with varying severity of atopic dermatitis

2021 ◽  
Vol 16 (1) ◽  
pp. 20-26
Author(s):  
S.M. Nedelska ◽  
D.O. Vakula
Keyword(s):  
Immune Response ◽  
Atopic Dermatitis ◽  
Age Groups ◽  
Statistical Processing ◽  
Serum Levels ◽  
Young Age ◽  
Control Group ◽  
Healthy Children ◽  
Th2 Immune Response ◽  
Low Levels

Background. Atopic dermatitis (AD) is the most common allergic disease among children of young age. Severe forms of AD with skin bacterial and fungal overgrowth may be associated with features of the immune response in different age groups. Plenty of studies demonstrated not only polarization of the Th2 immune response in AD patients, but also the Th1 immune dysregulation. The purpose of this study was to investigate the features of the immune response in children with varying severity of atopic dermatitis. Materials and methods. The study included 85 children aged 3 months to 3 years with a verified diagnosis of AD, living in the Zaporozhzhia region. Twenty healthy children without atopy formed a control group. The patients were divided into groups depending on the severity of AD based on the SCORAD scale. The serum levels C3, C4–2, CD3+, CD19–, CD4+, CD8–, CD4–, CD8+, CD3–, CD56+, CD19+, CD14, CD45, IgA, IgM, IgG, IgE, С3, С4–2, phagocytic acti­vity of neutrophils, and proliferative activity of lymphocytes were measured by flow cyto­metry (Synevo). ELISA method was used to detect serum levels of IL-13 (ELISA Kit, Thermo Fisher Scientific, Austria). Statistical processing of the results was performed using the official software package Statistica 13.0. Results. The study revealed eosinophilia in 60 % of the children with a mild and moderate course of AD (Me 5.62 [3.64; 7.81]) and in 56 % of the children with a severe course (Me 6.18 [3.13; 9.42]). The children with a severe course of AD and low levels of IL-13, C3 had transient hypogammaglobulinemia and significantly lower le­vels of the C3 complement, increased levels of CD4+, CD8– with simultaneously decreased levels of CD4–, CD8+ compared with groups of the children with high levels of ­IL-13 (р < 0.05). Conclusion. The results suggest that severe forms of AD in children of the young age were associated with changes in the complement system and low levels of cytotoxic cells, transient hypogammaglobulinemia. It requires deeper research of the cascades of the immune response in children with AD.

scholarly journals Serum Levels of Selected Th17 and Th22 Cytokines in Psoriatic Patients

2013 ◽  
Vol 35 ◽  
pp. 625-631 ◽  
Author(s):  
Anna Michalak-Stoma ◽  
Joanna Bartosińska ◽  
Małgorzata Kowal ◽  
Maria Juszkiewicz-Borowiec ◽  
Agnieszka Gerkowicz ◽  
...  
Keyword(s):  
Inflammatory Disease ◽  
Serum Levels ◽  
Skin Lesions ◽  
Psoriatic Skin ◽  
Control Group ◽  
Healthy Controls ◽  
T Helper ◽  
Systemic Inflammatory Disease ◽  
Pasi Score ◽  
Positive Correlations

Introduction. Psoriasis is a T cell-mediated inflammatory disease in which pathogenesis T helper (Th) lymphocytes (Th1, Th17, and Th22) play an important role. The aim of the study was to assess the serum levels of some cytokines involved in the Th17 and Th22 responses in psoriatic patients.Material and Methods. The study comprised 60 psoriatic patients and 30 healthy controls. In the serum collected from psoriatic patients and healthy controls, the concentrations of IL-6, IL-12, IL-17, IL-20, IL-22, and IL-23 were examined with ELISA kits. Severity of psoriatic skin lesions was assessed by means of PASI, BSA, and PGA scores.Results. IL-6, IL-20, and IL-22 concentrations were significantly higher in psoriatic patients in comparison with the control group. The positive correlations between the concentrations of IL-22 and IL-20 and severity of psoriasis assessed with PASI and BSA scores as well as IL-17 and PASI score were found. There was also a positive correlation between IL-23 and IL-17 concentrations.Conclusions. Results of the conducted studies suggest that Th22 response may contribute to the skin and systemic inflammatory disease in psoriasis. It seems that early identification of soluble biomarkers and initiation of well-matched treatment may prevent exacerbation and progression of psoriasis.

The effects of adenoidectomy of serum insulin-like growth factor-1 (IGF-1) and ghrelin in hypertrophied adenoids in children with otitis media with effusion

2020 ◽  
Vol 74 (4) ◽  
pp. 13-17
Author(s):  
Beata Żelazowska-Rutkowska ◽  
Klaudia Jacewicz ◽  
Edwina Kasprzycka ◽  
Bożena Skotnicka ◽  
Bogdan Cylwik
Keyword(s):  
Growth Factor ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Reference Group ◽  
Serum Insulin ◽  
Serum Levels ◽  
Control Group ◽  
Healthy Children ◽  
Insulin Like Growth Factor ◽  
Significant Difference

<b>Aim:</b> The aim of the current study was to assess the serum levels of insulin-like growth factor-1 (IGF-1) and ghrelin in hypertrophied adenoids in children suffering with or without otitis media with effusion before and after adenoidectomy. <br><b>Material and methods:</b> Serum IGF-1 and ghrelin concentrations were measured with specific enzyme-linked immunoassay (ELISA) methods. The study was carried out in 20 children with otitis media with effusion. The reference group comprised 24 children with hypertrophied adenoid, while control group included 19 children. <br><b>Results:</b> This mean values of IGF-1 in children with otitis media with effusion and children with hypertrophied adenoid before adenoidectomy were significantly lower than those found in healthy children. Serum levels of IGF-1 were higher after adenoidectomy. There was a significant difference of serum ghrelin levels between both examined groups and the control group. <br><b>Conclusion:</b> Our results suggest that adenoidectomy in children with hypertrophied adenoids and in children with otitis media with effusion significantly increases the level of IGF-1 in serum compared to before surgery through the effect of the GH-IGF-1 axis, which could contribute to children’s growth.

scholarly journals Possible Role of Heat Shock Protein 70 in Childhood Seizures

2018 ◽  
Vol 05 (02) ◽  
pp. 087-091
Author(s):  
Magdy Mostafa Kamel ◽  
Samir Mohammed Mounir ◽  
Nagwa Ismail Okaily ◽  
Mohammed Hosny Abdelzaher ◽  
Mohammed Hosny Hassan
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Febrile Seizures ◽  
Serum Levels ◽  
Cutoff Point ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Children ◽  
Hsp70 Level ◽  
Group I

Abstract Background There has been a long interest in investigating the relationship between heat shock protein (HSP) expression and the evidence of neuronal damage in the most susceptible brain areas after seizures. So, the present study aimed to assess heat shock protein (HSP70) in children with seizures (febrile seizures and epilepsy), and to find out the cutoff point of this marker that may help in confirming epilepsy diagnosis. The present study has been conducted to evaluate serum levels of HSP70 in children with epileptic and febrile seizures and to compare these results to that of healthy children. Materials and Methods A prospective study included 85 children (32 females and 53 males) in Children and Maternity Unit, Minia University Hospital, Minia, Egypt. Children were subdivided into three groups, group (I) included 30 children with epilepsy, group (II) included 30 children with febrile seizures, and group (III) included 25 healthy children that served as a control group. HSP70 assay was performed for all included children using the enzyme-linked immunosorbent assay technique. Results The overall results revealed significant high serum HSP70 levels in epilepsy and febrile seizures groups when compared with control group (p < 0.001). Also, HSP70 serum levels were significantly higher in epilepsy group than in febrile seizures group (p < 0.001). Serum HSP70 level at a cutoff point > 170 ng/L showed 60% sensitivity and specificity equal to 83.3% in prediction of epilepsy. Conclusion HSP70 level was significantly higher in epileptic and febrile seizures children than normal healthy children, and HSP70 may be beneficial in confirming the diagnosis of epilepsy.

Is It Necessary to Measure Thyroid Hormone Levels in Children Receiving Antiepileptic Drugs?

2018 ◽  
Vol 07 (04) ◽  
pp. 136-141
Author(s):  
Reem A. Abdel Aziz ◽  
Mostafa A. Abu ELela
Keyword(s):  
Antiepileptic Drugs ◽  
Thyroid Function ◽  
Thyroid Function Test ◽  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Control Group ◽  
Thyroid Function Tests ◽  
Healthy Children ◽  
Function Tests ◽  
Serum T3

Purpose This article evaluates the effect of antiepileptic drugs (AEDs) on thyroid function tests in children with epilepsy. Methodology One hundred twenty children were studied, with 40 patients taking older generation AEDs Na valproate (N = 20) and carbamazepine (N = 20), 40 patients taking newer generation AEDs levetiracetam (N = 20) and oxcarbazepine (N = 20), and 40 healthy children as controls. Serum T3, T4, FT4, and thyroid-stimulating hormone (TSH) were measured. Results Patients taking Na valproate had lower T3, T4, and a higher TSH level than controls. Patients taking carbamazepine had the lowest T3, T4, and FT4 serum levels among patients receiving AEDs. Conclusion The thyroid function tests were affected by the use of Na valproate and carbamazepine compared to both the control group and the group taking newer AEDs oxcarbazepine and levetiracetam. In addition, there was no effect of levetiracetam on thyroid function test results.

scholarly journals IL-17A, IL-22, IL-6, and IL-21 Serum Levels in Plaque-Type Psoriasis in Brazilian Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Priscilla Stela Santana de Oliveira ◽  
Pablo Ramon Gualberto Cardoso ◽  
Emerson Vasconcelos de Andrade Lima ◽  
Michelly Cristiny Pereira ◽  
Angela Luzia Branco Pinto Duarte ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Severity Index ◽  
Serum Levels ◽  
Statistical Correlation ◽  
Control Group ◽  
Inflammatory Skin Disease ◽  
Serum Samples ◽  
Cytokine Detection ◽  
Plaque Type ◽  
Pasi Score

Psoriasis is a chronic inflammatory skin disease characterized by alterations in cytokines produced by both Th1 and Th17 pathways. The aim of this study was to evaluate serum levels of pivotal cytokines and correlate them with clinical parameters. Serum samples from 53 psoriasis patients and 35 healthy volunteers, matched by the proportion of sex and age ratios, were collected for ELISA cytokine detection. Psoriasis Area and Severity Index (PASI) was assessed at the time of sampling in psoriasis patients. Our findings demonstrate that IL-17A, IL-22, and IL-6 serum concentrations were significantly higher in psoriasis patients than in the control group. No statistical correlation could be found between cytokines concentrations, PASI score, and age in this study. Although our results do not show any correlation between serum levels of IL-17A, IL-22, and IL-6 and disease activity, the present study confirms that they were increased in Brazilian psoriasis patients in comparison to healthy volunteers.

scholarly journals Are vitamin D and vitamin D receptor levels different in children with developmental dysplasia of the hip?

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Duran Topak ◽  
Muhammet Seyithanoğlu ◽  
Fatih Doğar ◽  
Ali Aydın Karadeniz ◽  
Burak Tanrıverdi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Serum Levels ◽  
Developmental Dysplasia ◽  
Laboratory Method ◽  
Control Group ◽  
Healthy Children ◽  
Genetic Transmission ◽  
Healthy Control ◽  
Dysplasia Of The Hip

Abstract Introduction Developmental dysplasia of the hip (DDH) is a common disorder and associated with significant morbidity of the hip joint. Several risk factors have been identified for DDH. The aim of this study is to investigate whether vitamin D and vitamin D receptor (VDR) levels differ in children with DDH and whether they have an effect on DDH development. Materials and methods A total of 40 (17 males, 23 females; 9 right hips, 16 left hips, 15 bilateral hips) children who were treated for developmental dysplasia and 40 (23 males, 17 females) healthy children without any musculoskeletal system and metabolic disorders were included in this study between January and June 2019. Blood samples from the DDH and control groups of children were collected to measure the serum levels of vitamin D, VDR, calcium (Ca), phosphorus (P), and alkaline phosphatase (ALP). The levels of Ca, P, and ALP were analyzed using the automated standard spectrophotometric laboratory method. The levels of vitamin D and VDR in the samples were analyzed using enzyme-linked immunoassay. Results There were no significant differences in the serum levels of Ca, P, ALP, and vitamin D between the DDH and healthy groups (Ca 9.96 ± 0.47 vs. 9.92 ± 0.48 mg/dL, respectively, p = 0.721; P 5.3 ± 0.94 vs. 4.82 ± 0.88 mg/dL, respectively, p = 0.23; ALP 252.22 ± 170.15 vs. 245.3 ± 130.93 U/L, respectively, p = 0.839). However, serum VDR levels were significantly lower in children in the DDH group (5.77 ± 3.51 ng/mL) than in the healthy control group (9.25 ± 6.43 ng/mL) (p = 0.004). Conclusions In conclusion, we believe that low VDR levels can affect DDH regardless of the serum levels of Ca, P, ALP, and vitamin D. More comprehensive studies involving parents are needed to understand whether VDR levels mediate genetic transmission in DDH or not.

scholarly journals Elevated Serum C1q Levels in Children With Sepsis

2021 ◽  
Vol 9 ◽  
Author(s):  
Huan Li ◽  
Juanjuan Chen ◽  
Yuanhui Hu ◽  
Xin Cai ◽  
Pingan Zhang
Keyword(s):  
Bacterial Infections ◽  
Viral Infections ◽  
Laboratory Data ◽  
Elevated Serum ◽  
Reference Value ◽  
Serum Levels ◽  
Critical Group ◽  
Control Group ◽  
Healthy Children ◽  
Complement C1q

Objective: To analyze the serum complement C1q levels in children with sepsis, and explore the suggestive effect of serum C1q levels on the condition of children with sepsis.Methods: The clinical and laboratory data of children with sepsis (n = 95) and healthy children (n = 71) in Renmin Hospital of Wuhan University from January 2019 to October 2019 were collected, and each index of the two groups was compared. Then we divided children with sepsis into three subgroups based on the Pediatric Critical Illness Score (PCIS): non-critical group, critical group, and extremely critical group. The serum C1q and PCT levels of the three subgroups were analyzed, and the correlation analysis was carried out between the levels of serum C1q and PCT levels as well as PCIS among children with sepsis. Finally, we analyzed the serum C1q levels of septic children infected by different pathogens.Results: The serum C1q levels of children with sepsis were significantly higher than those of healthy children (median 198.4 vs. 186.2 mg/L, P &lt; 0.001). In the analysis of subgroups, the serum C1q levels of non-critical group, critical group, and extremely critical group septic children were 182.80 (166.75, 195.85) mg/L, 219.90 (209.10, 246.40) mg/L and 249.95 (239.10, 272.25) mg/L, respectively, which were correlated with the severity of the disease. At the same time, we also found that serum C1q in children with sepsis was positively correlated with PCT levels (r = 0.5982, P &lt; 0.001), and negatively correlated with PCIS score (r = −0.6607, P &lt; 0.001). The serum C1q levels of septic children with bacterial infections, mycoplasma infections, viral infections, and co-infection were higher than those of the control group (P &lt; 0.05).Conclusion: The serum levels of C1q in children with sepsis were increased and related to the severity of sepsis, suggesting that C1q may be involved in the occurrence and development of sepsis, which had reference value for the preliminary diagnosis and severity classification of sepsis.

scholarly journals Antiviral and anti-inflammatory effects of medicated thread moxibustion combined with Shujin Jiannao granule in the treatment of HFMD

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Yue Yan Huang
Keyword(s):  
Western Medicine ◽  
Combined Treatment ◽  
Clinical Effectiveness ◽  
Serum Levels ◽  
Normal Control Group ◽  
Control Group ◽  
Healthy Children ◽  
Effectiveness Rate ◽  
Anti Inflammatory ◽  
Experimental Group

Objective To investigate antiviral and anti-inflammatory effects of medicated thread moxibustion combined with Shujin Jiannao granule in the treatment of HFMD. Methods A total of 124 children with HFMD were collected to divided into western medicine group (62 cases) and experimental group(62 cases) while 62 healthy children were enrolled to become normal control group. Western medicine group was treated according to conventional therapy while experimental group was treated with combined treatment of traditional Chinese medicine(TCM) and western medicine. To determine serum levels of S100B, NSE, CRP and WBC and observe the clinical effectiveness. Results In the western medicine group the total effectiveness rate is 88.7% while it is 96.8% in the experimental group（P<0.05）; the average disappearance time of symptoms are shorter in the experimental group than that in the western medicine group（P<0.05）. Compared with the control group，the serum levels of S100B, NSE, CRP and WBC all rise in HFMD children（P<0.05 or P<0.01）. After treatment , the serum levels of S100B, NSE, CRP and WBC reduce in both the experimental group and the western medicine group, when compared with that before treatment.The difference is statistically significant（P<0.05 or P<0.01）. 

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