scholarly journals Physicochemical characterization of C-phycocyanin from Plectonema sp. and elucidation of its bioactive potential through in silico approach

2022 ◽  
Vol 67 (4) ◽  
pp. 68-82
Author(s):  
Arbab Husain ◽  
Alvina Farooqui ◽  
Afreen Khanam ◽  
Shubham Sharma ◽  
Sadaf Mahfooz ◽  
...  
Keyword(s):  
Free Radicals ◽  
In Silico ◽  
Active Sites ◽  
Glycogen Synthase ◽  
Physicochemical Characterization ◽  
Peak Time ◽  
Spectroscopic Techniques ◽  
Ft Ir ◽  
Bioactive Potential

C-phycocyanin (C-PC), the integral blue-green algae (BGA) constituent has been substantially delineated for its biological attributes. Numerous reports have illustrated differential extraction and purification techniques for C-PC, however, there exists paucity in a broadly accepted process of its isolation. In the present study, we reported a highly selective C-PC purification and characterization method from nontoxic, filamentous and non-heterocystous cyanobacterium Plectonema sp. C-PC was extracted by freeze-thawing, desalted and purified using ion-exchange chromatography. The purity of C-PC along with its concentration was found to be 4.12 and 245 µg/ml respectively.  Comparative characterization of standard and purified C-PC was performed using diverse spectroscopic techniques namely Ultra Violet-visible, fluorescence spectroscopy and Fourier transform infrared (FT-IR). Sharp peaks at 620 nm and 350 nm with UV-visible and FT-IR spectroscopy respectively, confirmed amide I bands at around 1638 cm-1 (C=O stretching) whereas circular dichroism (CD) spectra exhibited α-helix content of secondary structure of standard 80.59% and 84.59% of column purified C-PC. SDS-PAGE exhibited two bands of α and β subunits 17 and 19 kDa respectively. HPLC evaluation of purified C-PC also indicated a close resemblance of retention peak time (1.465 min, 1.234 min, 1.097 min and 0.905 min) with standard C-PC having retention peak timing of 1.448 min, 1.233 min and 0.925 min. As a cautious approach, the purified C-PC was further lyophilized to extend its shelf life as compared to its liquid isoform. To evaluate the bioactive potential of the purified C-PC in silico approach was attempted. The molecular docking technique was carried out of C-PC as a ligand-protein with free radicals and α-amylase, α-glucosidase, glycogen synthase kinase-3 and glycogen phosphorylase enzymes as receptors to predict the free radical scavenging (antioxidant) and to target antidiabetic property of C-PC. In both receptors free radicals and enzymes, ligand C-PC plays an important role in establishing interactions within the cavity of active sites. These results established the antioxidant potential of C-PC and also give a clue towards its antidiabetic potential warranting further research.

Diffuse Reflectance FT-IR Characterization of Active Sites under Reaction Conditions: The Production of Oxygenates in the CO/H2 Reaction

1994 ◽  
Vol 48 (10) ◽  
pp. 1208-1212 ◽  
Author(s):  
J. J. Benítez ◽  
I. Carrizosa ◽  
J. A. Odriozola
Keyword(s):  
Infrared Spectra ◽  
Active Sites ◽  
Diffuse Reflectance ◽  
Reaction Conditions ◽  
In Situ Drifts ◽  
Ft Ir

The reactivity of a Lu2O3-promoted Rh/Al2O3 catalyst in the CO/H2 reaction is reported. Methane, heavier hydrocarbons, methanol, and ethanol are obtained. In situ DRIFTS has been employed to record the infrared spectra under the actual reaction conditions. The structure of the observed COads DRIFTS bands has been resolved into its components. The production of oxygenates (methanol and ethanol) has been correlated with the results of the deconvolution calculation. Specific sites for the production of methanol and ethanol in the CO/H2 reaction over a Rh,Lu2O3/Al2O3 catalyst are proposed.

scholarly journals THE PHYSICOCHEMICAL CHARACTERISTICS OF RECYCLED-PLASTIC PELLETS OBTAINED FROM DISPOSABLE FACE MASK WASTES

2021 ◽  
Vol 23 (1) ◽  
pp. 16
Author(s):  
Vienna Saraswaty ◽  
Rossy Choerun Nissa ◽  
Bonita Firdiana ◽  
Akbar Hanif Dawam Abdullah
Keyword(s):  
Tensile Strength ◽  
Physicochemical Characterization ◽  
Face Mask ◽  
Physicochemical Characteristics ◽  
Scanning Calorimetry ◽  
Elongation At Break ◽  
Ft Ir ◽  
The Government ◽  
Plastic Pellets

THE PHYSICOCHEMICAL CHARACTERISTICS OF RECYCLED-PLASTIC PELLETS OBTAINED FROM DISPOSABLE FACE MASK WASTES. The government policy to wear a face mask during the COVID-19 pandemic has increased disposable face mask wastes. Thus, to reduce such wastes, it is necessary to evaluate the physicochemical characteristics of disposable face masks wastes before the recycling process and the recycled products. In this study, physicochemical characterization of the 3-ply disposable face masks and the recycled plastic pellets after disinfection using 0.5% v/v sodium hypochlorite were evaluated. A set of parameters including the characterization of surface morphology by a scanning electron microscope (SEM), functional groups properties by a fourier transform infra-red spectroscopy (FT-IR), thermal behavior by a differential scanning calorimetry (DSC), tensile strength and elongation at break were evaluated. The surface morphological of each layer 3-ply disposable face mask showed that the layers were composed of non-woven fibers. The FT-IR evaluation revealed that 3-ply disposable face mask was made from a polypropylene. At the same time, the DSC analysis found that the polypropylene was in the form of homopolymer. The SEM analysis showed that the recycled plastic pellets showed a rough and uneven surface. The FT-IR, tensile strength and elongation at break of the recycled plastic pellets showed similarity with a virgin PP type CP442XP and a recycled PP from secondary recycling PP (COPLAST COMPANY). In summary, recycling 3-ply disposable face mask wastes to become plastic pellets is recommended for handling disposable face mask wastes problem.

scholarly journals GREEN SYNTHESIS AND SPECTROSCOPIC CHARACTERIZATION OF IRON (III) COMPLEX OF UREA FROM HUMAN URINE AND IRON RUST

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
V.O. Uduah ◽  
J.J. Gongden ◽  
M.L. Kagoro ◽  
K.K. Gurumyen ◽  
Y.N. Lohdip ◽  
...  
Keyword(s):  
Human Urine ◽  
Synthesis Method ◽  
Reaction Medium ◽  
Spectroscopic Characterization ◽  
Waste Materials ◽  
Spectroscopic Techniques ◽  
Distilled Water ◽  
Oxide Composition ◽  
Ft Ir

This work presents a dry synthesis of Iron (III) complex with urea isolated from human urine and Fe (III) obtained from iron rust particles. Iron (III), PI (Purified iron rust), was isolated from iron rust in 10% hydrochloric acid, HCl and distilled water respectively. The complex was synthesized via dry-synthesis method using the melted urea as reaction medium. The isolated Fe (III) was characterized by elemental analysis which was done using XRF Cu-Zn method. The complex was prepared in a 1:4 metal to ligand (M-L) ratio. The stoichiometry of reaction indicate a 1:3 ratio of M-L (Fe-U). The complex was characterized by FT-IR, UV-vis, XRF and XRD spectroscopic techniques. The Fe (III) isolate and Fe-U complex shows percentage yields of 35.7% and ~92% respectively. The elemental and oxide composition of Fe and Fe2O3 (i.e., PI) were 40.387% and 57.753% respectively. The results obtained from the characterization of the iron-urea complex, IUC, indicate FT-IR result as symmetric and asymmetric frequencies with peaks of a combination band of Vs (NH) and Vas (NH), C=O and V (C-N) all stretched, XRD showed the crystal to be amorphous. The elemental and oxide composition of the Fe and Fe2O3 in IUC were 40.007 and 44.201 respectively. The results obtained revealed that useful complexes can be synthesized easily from waste materials, such as urine and iron rust particles, which complement Green chemistry.

Steroid Compounds Isolation from Carthamus tinctorius Linn as Antimalarial

2021 ◽  
pp. 5297-5304
Author(s):  
Rini Hamsidi ◽  
Wahyuni Wahyuni ◽  
Adryan Fristiohady ◽  
Muhammad Hajrul Malaka ◽  
Idin Sahidin ◽  
...  
Keyword(s):  
Antimalarial Activity ◽  
Carthamus Tinctorius ◽  
Spectroscopic Techniques ◽  
1H And 13C Nmr ◽  
Marker Compound ◽  
Ic50 Value ◽  
Ft Ir ◽  
Ir Spectroscopic

Carthamus tinctorius Linn, also known as safflower, is a plant with the potential of being used in the production of antimalarial drugs. The purpose of this study was to isolate and identify the steroid compounds in the safflower and determine its antimalarial activity in vitro. The isolation process was conducted through extraction and chromatography methods. Then, the characterization of the isolated compounds was conducted through spectroscopic techniques which include Fourier Transform Infrared Spectroscopy (FT-IR), NMR 1-D (1H and 13C-NMR), and NMR 2-D (HMQC, HMBC, and H-H COZY) as well as comparing data with the existing literatures. In addition, the tests conducted were with variations of isolate concentrations (10, 1, 0.1, 0.01, and 0.001 μg/mL) against 3D7 strain of Plasmodium falciparum. Based on the FT-IR spectroscopic data, the steroid compounds isolated from safflowers might be stigmasterols. In addition, the isolates had -OH functional group in the region of 3431 cm-1, C-O in the region of 1053 cm-1, and Csp3-H in regions of 2960, 2934, and 2865 cm-1. The NMR 1-D data showed presence of 29 carbon atoms, while the protons were 48 in number. Furthermore, the IC50 value of the compound was 34.03 μg/mL with a percentage inhibition of 43.92% against the growth of P. falciparum. Therefore, it was classified as inactive agent in inhibiting the growth of malaria parasites, however, it could be used as a marker compound in C. tinctorius Linn extract.

scholarly journals Synthesis, Crystal Structures, and Spectroscopic Characterization of Bis-aldehyde Monomers and Their Electrically Conductive Pristine Polyazomethines

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1498 ◽  
Author(s):  
Abdul Hafeez ◽  
Zareen Akhter ◽  
John F. Gallagher ◽  
Nawazish Ali Khan ◽  
Asghari Gul ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Spectroscopic Characterization ◽  
Spectroscopic Techniques ◽  
Conductivity Measurements ◽  
X Ray Diffraction ◽  
Electrically Conductive ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Ft Ir

Bis-aldehyde monomers 4-(4′-formyl-phenoxy)benzaldehyde (3a), 3-methoxy-4-(4′-formyl-phenoxy)benzaldehyde (3b), and 3-ethoxy-4-(4′-formyl-phenoxy)benzaldehyde (3c) were synthesized by etherification of 4-fluorobenzaldehyde (1) with 4-hydroxybenzaldehyde (2a), 3-methoxy-4-hydroxybenzaldehyde (2b), and 3-ethoxy-4-hydroxybenzaldehyde (2c), respectively. Each monomer was polymerized with p-phenylenediamine and 4,4′-diaminodiphenyl ether to yield six poly(azomethine)s. Single crystal X-ray diffraction structures of 3b and 3c were determined. The structural characterization of the monomers and poly(azomethine)s was performed by FT-IR and NMR spectroscopic techniques and elemental analysis. Physicochemical properties of polymers were investigated by powder X-ray diffraction, thermogravimetric analysis (TGA), viscometry, UV–vis, spectroscopy and photoluminescence. These polymers were subjected to electrical conductivity measurements by the four-probe method, and their conductivities were found to be in the range 4.0 × 10−5 to 6.4 × 10−5 Scm−1, which was significantly higher than the values reported so far.

scholarly journals Efficient Synthesis and Characterization of Some Novel Nitro-Schiff Bases and Their Complexes of Nickel(II) and Copper(II)

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Hossein Naeimi ◽  
Mohsen Moradian
Keyword(s):  
Schiff Base ◽  
Schiff Base Complexes ◽  
Synthesis And Characterization ◽  
Planar Geometry ◽  
Hydroxyl Groups ◽  
Spectroscopic Techniques ◽  
Spectral Techniques ◽  
Chemical Structures ◽  
Ft Ir

Synthesis and characterization of some new Schiff base ligands derived from various diamines and nitrosalicylaldehyde and their complexes of Ni(II) and Cu(II) are reported. Several spectral techniques such as UV-Vis, FT-IR, and NMR spectra were used to identify the chemical structures of the reported ligands and their complexes. The ligands are found to be bound to the metal atom through the oxygen atoms of the hydroxyl groups and nitrogen atoms of imine groups, which is also supported by spectroscopic techniques. The results obtained by FT-IR and NMR showed that the Schiff base complexes of transition metal (II) have square-planar geometry.

scholarly journals Physicochemical Characterization of Alginate Beads Containing Sugars and Biopolymers

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Tatiana Aguirre Calvo ◽  
Patricio Santagapita
Keyword(s):  
Water Content ◽  
Digital Image Analysis ◽  
Individual Component ◽  
Physicochemical Characterization ◽  
Alginate Beads ◽  
Complete Characterization ◽  
Ft Ir ◽  
Different Characteristics ◽  
Lower Water Content

Alginate hydrogels are suitable for the encapsulation of a great variety of biomolecules. Several alternatives to the conventional alginate formulation are being studied for a broad range of biotechnological applications; among them the addition of sugars and biopolymers arises as a good and economic strategy. Sugars (trehalose and β-cyclodextrin), a cationic biopolymer (chitosan), an anionic biopolymer (pectin), and neutral gums (Arabic, guar, espina corona, and vinal gums) provided different characteristics to the beads. Here we discuss the influence of beads composition on several physicochemical properties, such as size and shape, analyzed through digital image analysis besides both water content and activity. The results showed that the addition of a second biopolymer, β-CD, or trehalose provoked more compact beads, but the fact that they were compact not necessarily implies a concomitant increase in their circularity. Espina corona beads showed the highest circularity value, being useful for applications which require a controlled and high circularity, assuring quality control. Beads with trehalose showed lower water content than the rest of the system, followed by those containing galactomannans (espina corona, vinal, and guar gums), revealing polymer structure effects. A complete characterization of the beads was performed by FT-IR, assigning the characteristics bands to each individual component.

scholarly journals Physicochemical Characterization Of The Multiuse Medical Textiles In Surgery And As Packaging Material In Medical Sterilization

2017 ◽  
Vol 17 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Beti Rogina-Car ◽  
Sandra Flincec Grgac ◽  
Drago Katovic
Keyword(s):  
Fourier Transform ◽  
Structural Characteristics ◽  
Physicochemical Characterization ◽  
Morphological Characterization ◽  
Packaging Material ◽  
Medical Textiles ◽  
Ft Ir ◽  
Continuous Use ◽  
Electronic Microscope

AbstractThis work investigates changes in the physicochemical properties of dry multiuse medical textiles used in surgery and as packaging material in sterilization after 0, 1, 10, 20, 30, and 50 washing and sterilization cycles in real hospital conditions of the Clinical-Hospital Centre in Zagreb. Scanning electronic microscope (SEM) was used to perform morphological characterization. Physicochemical characterization and the resulting changes in the medical textiles were monitored using Fourier transform infrared (FT-IR) spectrometer. The change in the mass of the medical textiles as a results of temperature was determined by thermogravimetric (TG) analysis. Furthermore, structural characteristics based on the changes that resulted during the washing and sterilization processes are provided. The conclusion of the conducted research on the changes in the properties of multiuse medical textiles (Cotton/PES, Tencel®, and three-layer PES/PU/PES textile laminate) in real hospital conditions is that the medical textiles do manage to preserve properties after continuous use and it is safe to use them up to 50 washing and sterilization cycles.

Physicochemical Characterization of Oxidatively Degraded Calcium Lignosulfonate via Alkaline Hydrogen Peroxide

2014 ◽  
Vol 887-888 ◽  
pp. 575-580 ◽  
Author(s):  
Li Hong Hu ◽  
Jing Zhou ◽  
Cai Ying Bo ◽  
Bing Chuan Liang ◽  
Yong Hong Zhou
Keyword(s):  
Hydrogen Peroxide ◽  
Phenolic Resin ◽  
Physicochemical Characterization ◽  
Oxidative Degradation ◽  
Alkaline Condition ◽  
Alkaline Hydrogen Peroxide ◽  
Phenolic Contents ◽  
H Nmr ◽  
Ft Ir

In order to increase the application prospect of calcium lignosulfonate in phenolic resin, Calcium lignosulfonate was oxidatively degraded by Hydrogen peroxide under alkaline condition. Both lignins were characterized by FT-IR, 1H NMR, UV, GC-MS and GPC. The optimal degradation conditions are: lignosulfonate:water=1:1, wt/wt, pH=10, temperature 60 °C, reaction time 2 h, and H2O2 dosage 6 wt% (based on weight of lignosulfonate). The results show that the degraded resultant is higher in phenolic contents, and lower in methoxyl content. Number molecular weight (Mn) of calcium lignosulfonate sharply decreases to 2294, versus 17774 before oxidative degradation.Guaiacly monoer content increased and kinds of phenolic compounds occurre in oxidative degradation fractions, mainly due to β-O-4 and β-5 cleavage. And reactive activity of the oxidatively degraded compounds is sharply increased.

scholarly journals Synthesis and characterization of novel mono, bis and tris heteroaryl chalcone derivatives of 1,3,5-trimethoxybenzene

2021 ◽  
Vol 14 (1) ◽  
pp. 39-47
Author(s):  
M. Fatih Polat ◽  
Derya Aktaş Anı
Keyword(s):  
Elemental Analysis ◽  
High Purity ◽  
Synthesis And Characterization ◽  
Spectroscopic Techniques ◽  
Reaction Conditions ◽  
Chalcone Derivatives ◽  
Ft Ir ◽  
Derivatives Of

In this study, a new series consisting of 12 heteroaryl chalcone derivatives of 1,3,5-trimethoxybenzene were synthesised. Chalcones were synthesised in high purity and efficiency, via condensation of mono, bis and tris 2,4,6-trimethoxy acetophenones with hetero-2-carbaldehyde derivatives based on Claisen Schdmit condensation. The reactions feature a good scope for the all products, mild reaction conditions and good yields. The synthesized compounds were characterized by using FT-IR, NMR and elemental analysis spectroscopic techniques.

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