scholarly journals Assessment of toxicity on the basis of total phenolic content in oleander leaves (Nerium oleander L.) against Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae)

2018 ◽  
Vol 111 (2) ◽  
pp. 265 ◽  
Author(s):  
Billal NIA ◽  
Naama FRAH ◽  
Adel LEKBIR ◽  
Khadidja BENHMED
Keyword(s):  
Myzus Persicae ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Insect Pests ◽  
Ethanolic Extract ◽  
Nerium Oleander ◽  
Total Phenolic ◽  
Safe Alternative ◽  
Insecticidal Effect ◽  
Gallic Acid Equivalent

<p><em></em><em>Nerium oleander</em> is an evergreen flowering shrub or small tree distributed widely in the Mediterranean region. It is also a source of polyphenols and cardenolides ?? with insecticidal effect which could be a safe alternative of chemical control of insect pests. In the present work, five concentrations (0 %, 1 %, 2.5 %, 5 %, and 10 %) of ethanolic extract from <em>Nerium oleander </em>leaves<em> </em>were evaluated for its insecticidal effect against 3 to 4 days old <em>Myzus persicae </em>individuals under laboratory conditions. Obtained results showed a significant insecticidal effect with 70 % of mortality at the highest concentration (10 %). Total phenolic content of leaf ethanolic extract of this plant was 1721.36 mg gallic acid equivalent 100 g<sup>-1</sup> dry matter. The results obtained suggest that we could make bioinsecticides based on leaves ethanolic extracts from <em>N. oleander</em> which rich in polyphenols for use eventually in integrated pest management.</p>

scholarly journals Penetapan Kadar Fenolik Total Ekstrak Kacang Panjang (Vigna unguiculata) dengan Metode Spektrofotometri UV-Visible

2021 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Lalu Aang Robby Dewantara ◽  
Agus Dwi Ananto ◽  
Yayuk Andayani
Keyword(s):  
Gallic Acid ◽  
Aqueous Extract ◽  
Vigna Unguiculata ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Uv Visible ◽  
Gallic Acid Equivalent

ABSTRAKKacang panjang (Vigna unguiculata) merupakan salah satu tanaman yang berpotensi sebagai antioksidan karena mengandung metabolit sekunder hampir di seluruh bagian tanamannya, seperti pada daging buahnya. Penelitian ini bertujuan untuk menentukan kadar fenolik total ekstrak etanol dan ekstrak air daging buah kacang panjang dengan metode spektrofotometri UV-Visible. Sampel daging buah kacang panjang dimaserasi menggunakan dua pelarut berbeda, yaitu pelarut etanol 96% dan pelarut air. Analisis kadar fenolik total dilakukan dengan metode spektrofotometri UV-Visible. Kadar fenolik total dinyatakan dalam gallic acid equivalent (GAE). Hasil analisis menunjukkan bahwa kadar fenolik total ekstrak etanol kacang panjang sebesar 40,4173 ± 4.2303 mg GAE/g dan kadar fenolik total ekstrak air kacang panjang sebesar 60.6062 ± 2,8533 mg GAE/g. Ekstrak etanol dan ekstrak air dari daging buah kacang panjang berpotensi sebagai antioksidan dilihat dari parameter kadar fenolik totalnya. Berdasarkan hasil penelitian, diperoleh bahwa kadar fenolik total ekstrak air lebih tinggi dibandingkan ekstrak etanol daging buah kacang panjang. Kata kunci : Kacang panjang (Vigna unguiculata); Spektrofotometri UV-Visible; Kadar fenolik total.ABSTRACTCowpea (Vigna unguiculata) is one of the vegetables that have antioxidant potential due to it contains some secondary metabolites that scattered on every part of the Cowpea, such as the pod coat. The aimed of this study was to determine total phenolic content of Cowpea pod coat ethanolic extract and aqueous extract using UV-Vis spectrophotometry method. Cowpea pod coats were macerated using two different solvents, ethanol 96%, and aqueous.  Total phenolic content was showed as gallic acid equivalent (GAE). The analysis results showed that total phenolic content of ethanol extract of cowpea was 40,41732 ± 4.2303 mg GAE/g and total phenolic content of aqueous extract of cowpea was 60.6062 ± 2,8533 mg GAE/g. The ethanolic extract and aqueous extract of cowpea pod coat have antioxidant potential in terms of total phenolic content parameter. Based on the research, the total phenolic content of cowpea pod aqueous extract higher than cowpea pod coat ethanolic extract.Keywords : Cowpea (Vigna unguiculata); Spectrophotometric UV-Visible; Total phenolic content.

scholarly journals Polyphenols in Coreopsis tinctoria Nutt. fruits and the plant extracts antioxidant capacity evaluation

2014 ◽  
Vol 12 (8) ◽  
pp. 858-867 ◽  
Author(s):  
Christina Zălaru ◽  
Claudia Crişan ◽  
Ioan Călinescu ◽  
Zenovia Moldovan ◽  
Isabela Ţârcomnicu ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Optimum Operating ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Coreopsis Tinctoria

AbstractThe aim of this research was to investigate the chemical composition of Coreopsis tinctoria Nutt. fruits extract, to highlight the potential of ultrasound assisted extraction in the fast preparation of extracts rich in polyphenols using different solvents (55%, 78% and 96% hydrous ethanol) and to evaluate the antioxidant potential of formulated extracts. LC-MS/MS was used to characterize the ethanolic extract from Coreopsis tinctoria Nutt. dried fruits. The extract contains different flavonoids (marein, flavanomarein, quercetagetin-7-O-glucoside, okanin aurone, leptosidin, luteolin, apigenin) and phenolic acids (chlorogenic acid, caffeic acid). Several parameters that could affect extraction efficiency were evaluated. Finally, this study focused on determination of plant extracts total phenolic content and their antioxidant capacity. The experimental results allowed the selection of the optimum operating parameters leading to the highest total polyphenolic content, expressed as gallic acid equivalents, and avoiding the degradation of phenolic compounds (ethanol 55%; extraction temperature 323.15 K, extraction time 30 min, liquid/solid ratio 20/1). A good relationship between total phenolic content and antioxidant capacity was obtained.

Phytochemical Evaluation and In-vitro Antioxidant Potential of whole plant of Caralluma adscendens

2021 ◽  
pp. 2774-2778
Author(s):  
Divya Yada ◽  
T. Sivakkumar ◽  
M. Sudhakar
Keyword(s):  
Nitric Oxide ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Whole Plant

The current line of investigation was focused at perusing the presence of phytochemical constituents, investigation of total phenol and flavonoid content, the antioxidant potential of various extracts of Caralluma adscendens whole plant using various in-vitro assays. The dried plant powder was extracted with various solvents based on polarity (Pet ether, Chloroform, Ethyl acetate, Ethanol and Aqueous) by hot continuous extraction in Soxhlet's apparatus and Extracts were dried. Phytoconstituents present in each extract was examined by performing preliminary phytochemical screening. Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Antioxidant potential for crude extracts were studied by DPPH, nitric oxide scavenging and FRAP methods. The total phenolic content and flavonoid content of Ethanolic extract of plant was found to be 80.08±0.629mg and 70.88±1.170mg of GAE and Quercetin equivalents respectively. The Ethanolic extract exhibited potent antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH), nitric oxide scavenging and ferric reducing antioxidant power assays(FRAP) than the other extracts. The IC50 values for the Ethanolic extract of Caralluma adscendens was found to be 214.765±0.224 µg/ml and 215.928±0.506µg/ml by DPPH and nitric oxide scavenging assays respectively.

scholarly journals Nutraceutical and Functional Properties of Peel, Pulp, and Seed Extracts of Six ‘Köhnü’ Grape Clones

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 346
Author(s):  
Muhammed Kupe ◽  
Neva Karatas ◽  
Mehmet Settar Unal ◽  
Sezai Ercisli ◽  
Mojmir Baron ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Grape Juice ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Gallic Acid Equivalent ◽  
Black Color ◽  
Seed Extracts

Grape production has a long history in the Elazig province and surrounding vicinity and produced grapes have been used for table consumption and also processed into traditional beverages, Şıra (special non-alcoholic grape juice) and wine. In the Elazig province, the main grape cultivars are ‘Ağın Beyazı’, ‘Öküzgözü’, ‘Boğazkere’, ‘Şilfoni’, ‘Tahannebi’, and ‘Köhnü’. Among them, ‘Köhnü’ cultivar is highly preferred by consumers due to its black color and perfect berry characteristics. The cultivar has grown for centuries in different parts of Elazig and shows a great variability for most of its morphological and biochemical characteristics. In the present study, we aimed to determine morphological and biochemical traits in six ‘Köhnü’ clones sampled from Elazığ. The cluster weight of six clones was found between 334–394 g. The highest total phenolic content was observed in seeds followed by peel and pulp samples. The seed extract of Clone 2 had the highest total phenolic content at 254 mg gallic acid equivalent/100 g fresh weight. The results also showed that peel, pulp, and seed samples of ‘Köhnü’ grape clones had considerable amounts of antioxidant components determined by DPPH (1,1-Diphenyl-2-picryl-hydrazyl), FRAP (ferric reducing antioxidant power), and TEAC assays and might be rich sources of natural antioxidants. Among the six ‘Köhnü’ clones, Clone 3, and Clone 6 differed from the others in respect to the highest cluster weight, the highest concentrations of total phenolic content, and antioxidant activity. The results also implied that all clones could be used potentially as a readily accessible source of natural antioxidants and as a possible pharmaceutical supplement.

scholarly journals Phytochemical screening and in vitro anti-inflammatory activity of ethanolic extract of Epidendrum coryophorum leaves.

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1387-1393
Author(s):  
Irina Francesca González Mera ◽  
Orestes Darío López Hernández ◽  
Vivian Morera Córdova
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Inflammatory

Epidendrum coryophorum belongs to the Orchidaceae family. Traditional uses of some species for this genus include infusions of the leaves used for kidney problems, treat influenza, conjunctivitis, liver pain, relieve kidney symptoms, and hypoglycemic effect. This work's objective was to determine the phytochemical profile of the ethanolic extract of Epidendrum coryophorum leaves and to evaluate the potential anti-inflammatory activity in vitro of the extract employing the erythrocyte membrane stabilization method. The phytochemical screening carried out in this work suggested phenols, coumarins, flavonoids, tannins, steroids, and sterols in the ethanolic extract of Epidendrum coryophorum leaves. Cardiotonic glycosides and carbohydrates were also found. The ethanolic extract's UV-Vis spectrum showed absorption maxima at 268 nm and 332 nm, which could correspond to flavonoids of the flavonoid classes, 3-OH substituted flavonols, or isoflavones. The quantitative determination of total phenols of the ethanolic extract was carried out using the Folin-Ciocalteu method. The total phenolic content expressed as mg Gallic acid equivalent (G.A.E.) per gram of extract was found to be 19,96 mgGAE/g of Epidendrum coryophorum. The ethanolic extract of Epidendrum coryophorum leaves showed hemolysis inhibition values ​​of 18,19% at 1,0 mg/mL, 38,98% at 1,5 mg/mL and 40,94% at 2,5 mg/mL compared with aspirin (positive control) giving values ​​of 65,33% at 1,0 mg/mL, 72,26% at 1,5 mg/mL and 73,75% at 2,5 mg/mL. The values ​​obtained for inhibition of hemolysis with ethanolic extract, compared with the values ​​obtained with a pure anti-inflammatory, are significant and demonstrate anti-inflammatory activity in Epidendrum coryophorum. Keywords: Epidendrum coryophorum, total phenolic content, microencapsulation, anti-inflammatory activity

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals Neuropharmacological studies of ethanolic extract of Vaccinium corymbosum on Alzheimer’s type dementia and catatonia in Swiss albino mice

2021 ◽  
Vol 10 (2) ◽  
pp. 241-248
Author(s):  
Hanish Singh Jayasingh Chellammal ◽  
Bama VV Menon ◽  
Mizaton Hazizul Hasan ◽  
Afiq Azil ◽  
Muhammad Taufiq Bin Suhaimi ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Vaccinium Corymbosum ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Neuroprotective Effects ◽  
Behavioural Changes

Introduction: Neuroactive herbal drugs enriched with antioxidants are valuable in treating neurocognitive dysfunction and Vaccinium corymbosum, enriched with antioxidant phytochemicals, is used for treating memory disorders. Hence, the present study evaluated the neuroprotective effects of ethanolic extract of Vaccinium corymbosum (EEVC) on aluminium chloride(AlCl3)-induced Alzheimer’s type of dementia and haloperidol-induced catalepsy-associated behavioural changes. Methods:In vitro antioxidant potential was evaluated using 1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The total phenolic content (TPC) was quantified. For in vivo studies, AlCl3 (100 mg/kg) was orally administered for 42 days, whereas the EEVC was administered on the 21st day until the 42nd day in two doses (200 mg/kg and 400 mg/kg). In the haloperidol-induced group, EEVC was treated for 21 days, and haloperidol (1 mg/kg) was administered to induce behavioural changes. Open-field, Y-Maze and traction tests were performed, and the mice brain acetylcholinesterase (AChE) enzyme was determined. Results: IC50 values in DPPH and ABTS assays were 85.5 μg/mL and 80 μg/mL, respectively and the total phenolic content of EEVC was found to be 0.166 mg. In a behavioral study, animals treated with 200 mg/kg and 400 mg/kg of EEVC exhibited a neuroprotective impact on AlCl3-induced neurodegeneration and haloperidol-induced behavioral changes with significant inhibition (P < 0.05 and P < 0.01, respectively) in acetylcholinesterase enzyme. Conclusion: The neuroprotection by EEVC postulated that it is a promising therapeutic agent for treating behavioral and cognitive dysfunctions. Further investigations on pro-inflammatory cytokine and neuroendocrine regulation in transgenic Alzheimer’s disease (AD)models complement the therapeutic value of V. corymbosum.

scholarly journals PHARMACOGNOSTIC AND PHYTOCHEMICAL INVESTIGATION OF AILANTHUS EXCELSA ROXB. BARK

2020 ◽  
pp. 74-78
Author(s):  
RASIKA D BHALKE ◽  
MAHENDRA A GIRI
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Morphological Studies ◽  
Ailanthus Excelsa ◽  
Phytochemical Investigation ◽  
The Family ◽  
Physicochemical Evaluation ◽  
Gallic Acid Equivalent ◽  
Evaluation Parameters

Objective: The present investigation aims at detail pharmacognostic study of the bark of Ailanthus excelsa Roxb. (BAE). A. excelsa is an important plant in Indian system of medicine belonging to the family Simaroubaceae. Methods: BAE was studied for various pharmacognostic evaluation parameters such as examination of morphological and microscopic characters and physicochemical evaluation. The total phenolic content was determined using Folin–Ciocalteu reagent spectrophotometrically and calculated as gallic acid equivalent (GAE/g). Results: The morphological studies revealed that BAE is yellowish-brown in color with a smooth texture and characteristic odor. Microscopical studies indicated the presence of cork, cortex with a group of stone cells in BAE with secondary phloem region. Prismatic and acicular raphides calcium oxalate crystals were observed. The total ash value of BAE was found to be 8.95%. Bark powder was successively extracted in Soxhlet extractor using solvents from low polarity to high polarity such as petroleum ether, chloroform, ethyl acetate, and methanol. Alkaloids, glycosides, tannins, triterpenoids, carbohydrates, and flavonoids are present when the preliminary phytochemical investigation was done. The total phenolic content in BAE was found to be 9.95 mg GAE/g. Conclusion: The results obtained from the study provide information for proper identification and standardization of plant.

scholarly journals The antimicrobial activity of Prunella vulgaris extracts

2015 ◽  
Vol 61 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Atefeh Mahboubi ◽  
Nastaran Kazempour
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Prunella Vulgaris ◽  
Ethanol Extracts

Summary Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent.

scholarly journals Comparison of Phenolic Compounds and Antioxidant Activities of Raw and Cooked Turkish Artichoke Cultivars

2021 ◽  
Vol 5 ◽  
Author(s):  
Seda Kayahan ◽  
Didem Saloglu
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Acid Content ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Microwave Cooking ◽  
Gallic Acid Equivalent

The objective of this work was to determine the total phenolic compounds and antioxidants in raw and cooked Sakiz and Bayrampasa variety artichokes in parts such as inner bracts, stems, receptacles, and outer bracts. The artichokes were cooked by boiling, microwaving, and baking methods, and total phenolic compounds and antioxidants of cooked artichokes were evaluated. While TPC (total phenolic content), DPPH (2,2-diphenyl-1-picryl-hydrazine), and CUPRAC values for the leaves of raw Bayrampasa artichoke were found to be 686 mg gallic acid equivalent (GAE)/100 g, 478 mg TE/100 g, and 4,875 mg TE/100 g, respectively, TPC, DPPH, and CUPRAC values for stems of Sakiz artichoke were determined to be 1,579 mg GAE/100 g, 1,259 mg TE/100 g, and 3,575 mg TE/100 g. A significant increase in the content of TPC, DPPH, and CUPRAC values was observed for all cooking applications of both artichokes. DPPH and CUPRAC values increased by 11 and 43 times and 17 and 6.7 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively. Chlorogenic acid, cynarine, and cynaroside content of both artichokes had an increment after all cooking applications. Chlorogenic acid content was improved 29 and 58 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively.

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