scholarly journals Growth and antioxidant system responses of maize (Zea mays L.) seedling to different concentration of pyrene in a controlled environment

2019 ◽  
Vol 113 (1) ◽  
pp. 29 ◽  
Author(s):  
Mahdieh HOUSHANI ◽  
Seyed Yahya SALEHI-LISAR ◽  
Ali MOVAFEGHI ◽  
Ruhollah MOTAFAKKERAZAD

<p>Polycyclic aromatic hydrocarbons (PAHs) are a class of organic pollutants effecting different aspects of plants physiology. To assess the physiological responses of plants to PAHs, maize (<em>Zea mays</em>)<em> </em>was treated with 25, 50, 75, and 100 ppm of pyrene and after 21 days, the activity of some antioxidant enzymes, malondialdehyde (MDA), total flavonoid, total anthocyanin, and soluble sugar contents were measured in shoots and roots of plants. Pyrene led to increase MDA content as well as CAT, POD, and SOD activities. Increase in pyrene concentration reduced all studied growth variables and significantly increased photosynthetic pigments contents of plants. Soluble sugar content was significantly higher in the shoot, while that was reduced in the roots through increasing of pyrene concentration (<em>p &lt;</em> 0.05). Also, the increase of pyrene concentration decreased total flavonoid content compared to anthocyanin content. In conclusion, these findings supported the hypothesis that pyrene toxicity induces oxidative stress in the maize plant and it also increases the antioxidant systems in order to moderating stress condition. However, the antioxidant system of maize was not strong enough to eliminate all produced ROS at high concentrations, thus this caused oxidative damage to the plant and decreased its growth variables.</p>

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 71
Author(s):  
Mario Gabrielli ◽  
Vanessa Lançon-Verdier ◽  
Pierre Picouet ◽  
Chantal Maury

Table grape quality is of importance for consumers and thus for producers. Its objective quality is usually determined by destructive methods mainly based on sugar content. This study proposed to evaluate the possibility of hyperspectral imaging to characterize table grapes quality through its sugar (TSS), total flavonoid (TF), and total anthocyanin (TA) contents. Different data pre-treatments (WD, SNV, and 1st and 2nd derivative) and different methods were tested to get the best prediction models: PLS with full spectra and then Multiple Linear Regression (MLR) were realized after selecting the optimal wavelengths thanks to the regression coefficients (β-coefficients) and the Variable Importance in Projection (VIP) scores. All models were good at showing that hyperspectral imaging is a relevant method to predict sugar, total flavonoid, and total anthocyanin contents. The best predictions were obtained from optimal wavelength selection based on β-coefficients for TSS and from VIPs optimal wavelength windows using SNV pre-treatment for total flavonoid and total anthocyanin content. Thus, good prediction models were proposed in order to characterize grapes while reducing the data sets and limit the data storage to enable an industrial use.


2018 ◽  
Vol 3 (3) ◽  
pp. 99-103 ◽  
Author(s):  
Mohammad Ehsan Taghavizadeh Yazdi ◽  
Jalil Khara ◽  
Mohammad Reza Housaindokht ◽  
Hamid Reza Sadeghnia ◽  
Sedigheh Esmaeilzadeh Bahabadid ◽  
...  

Currently, there have been lots of interests in phytochemicals as bioactive components. The roles of fruit, vegetables, and red pigments in preventing diseases have been partly accredited to the antioxidant properties of their constituent polyphenols, flavonoid, anthocyanins, and etc. Biochemistry parameters including the relative levels of antioxidant activity, total phenolic content, total flavonoid, total anthocyanin, soluble and insoluble sugar content of Ribes khorasanicum, have been calculated in this project. The total anthocyanin content of the investigated Ribes khorasanicum measured to be 62.9 mg/g in dry weight, while displaying high levels when compared to other components, which indicates that the anthocyanin content was the predominant antioxidant components in the investigated plant. This particular plant has the potential to serve as the important source of antioxidant that can be utilized in different biological and medical applications.


2018 ◽  
Vol 46 (2) ◽  
pp. 639-652 ◽  
Author(s):  
Onur Tolga OKAN ◽  
İlhan DENİZ ◽  
Nurettin YAYLI ◽  
İhsan Güngör ŞAT ◽  
Mehmet ÖZ ◽  
...  

Commercial blueberry production has been a viable industry throughout the world for 95 years; because of blueberry is a good source of antioxidant. Blueberries are especially rich in anthocyanin, a flavonoid with potent antioxidant capacity. The aim of this study was to compare the phenolic quantities, antioxidant activities, anthocyanin, sugar and phenolic compounds of blueberries produced in Turkey with those of similar blueberry varieties produced around the world. As a result of the conducted analysis, the total phenolic content (TPC) amount found in the berries was 77.26-215.12 mg GAE/100 g, the total flavonoid content (TFC) was 30.44-91.69 mg QE/100 g and the total anthocyanin content (TAC) was 43.03-295.06 mg c3-GE/100 g. Examining the antioxidant activities of the berries, DPPH between 1.10-5.65 mg/ml, FRAP between 454.93-36832.96 µmol troloks/100 g, β-Carotene between 40.66-86.48%. It was determined that the natural berries contained much more phenolic compounds and higher antioxidant activity than that of the cultivars The result of HPLC analysis, chlorogenic acid is determined to be the dominant compound in all berries. Furthermore, fructose and glucose are found in all fruits in different quantities while sucrose is found in certain varieties of berries as well. At the end of the performed study the data indicate that wild and cultivars of blueberries are rich sources of antioxidants for local as well international industries importing this fruit for food processing and enormous products.


2021 ◽  
Vol 1033 ◽  
pp. 87-92
Author(s):  
Lilis Kistriyani ◽  
Lutfi Kurniawan ◽  
Fadhli Syaputra ◽  
Achmad Chafidz

The purpose of this study was to determine and compare the effect of oven drying and spray drying treatments on the encapsulation of anthocyanin, flavonoid and phenolic extracted from watermelon rind. The analysis done in this work were as follow: total anthocyanin (TA), total flavonoid (TF) and total phenolic (TPC) content in the supernatant, yield of nanocapsule, Particle Size Analysis, and In Vitro test (test for flavonoid and phenolic release) in the supernatant food. The analysis results showed that the total anthocyanin content in watermelon rind with a spray drying treatment was 0.1113 mg/L, total flavonoid was 0.6159 g/mL, and total phenolic was 0.3410 g/mL. While using the oven, the anthocyanin content was 0.0891 mg/L, total flavonoid was 1.4590 g/mL, and total phenolic was 1.1349 g/mL. As noticed, the total content of anthocyanin resulted by using a spray dryer was greater than by using an oven. However, the total flavonoid and total phenolic resulted by using a spray dryer was smaller than by using an oven. During In Vitro analysis, the results of the release of flavonoid and phenolic resulted by using an oven into the distilled water were much larger and unstable than by using a spray dryer. This is likely because chitosan-Na (TPP) bonds are weak. Additionally, the yield of capsule on the use of spray dryer was 0.6, higher than the use of an oven, which was 0.48. While, the average particle size in the use of a spray dryer was 2.296 μm, smaller than the use of an oven, which was 61.998 μm.


2019 ◽  
Vol 131 ◽  
pp. 01108
Author(s):  
Chun-hua Xia ◽  
Yi-wei Chen ◽  
Jian-hua Chen

Anthurium andraeanum Lind. is an important tropical flower. However, the spathe of A. andraeanum develops a ”greenback” under low-light conditions. This study aimed to evaluate the use of oyster shell supplementation as a cultivation technique for inhibiting the spathe “greenback” of A. andraeanum under low-light conditions. Appropriate calcium from micro-dissolution of oyster shell can improve the activity of phenylalanine ammonia lyase (PAL) which is positively correlated with the anthocyanin content. Using regression models and response surface methodology (RSM), the relationships between oyster shell fragments and the anthocyanin content were determined. The results showed that the solubility of oyster shells increased with the increase in fragment weight, C, and time, T at pH 5.9. In oyster shell substrate, cultivation of A. andraeanum under low-light conditions (less than 220 μmol·m−2·s−1) at pH 5.9, regression analysis showed that the PAL activity in pedicels of A. andraeanum first increased and then decreased with the increase in oyster shell fragment weight C, and the 286 mg oyster shell fragments greatly increased the PAL activity of A. andraeanum pedicels within 8 weeks (w). The 286 mg oyster shell fragments significantly increased the anthocyanin content in A. andraeanum spathes under weak-light conditions within 8 h, and the soluble sugar content reached the maximum value at 15 weeks as well, together with the pedicel diameter, soluble sugar and water content, thus inhibiting the spathe “greenback” of A. andraeanum.


2019 ◽  
Vol 20 (3) ◽  
pp. 363-379
Author(s):  
Mina El Baji ◽  
◽  
Ossama Kodad ◽  
Hafida Hanine ◽  
Said En-nahli ◽  
...  

Theobjective of thisstudyis to investigate the phenotypic and biochemicalparameters of selectedsweet cherry (Prunus avium) from middle region of Morocco. Themain biochemical composition, contentsof total polyphenols, total flavonoids, total anthocyanins and antioxidant activity, were measured in the fruits of four sweet cherry cultivars (ʻBurlat’, ʻVan’, ʻNapoleon’andʻCerisette’) grown in two locations (“Laanoceur”and “Toufselt”) in the MiddleAtlas. The free radical scavenging activity was evaluated spectrophotometrically using 1,1‒diphenyl‒2‒picrylhydrazine (DPPH)and ABTS assay.The fruit weight, and pulppercentagewerestudied and found to range between 237–329 g, 38.14–42.22%, 39.21–44.36g, and 52.27–57.48%, respectively.The total phenolic and total anthocyanin content rangedfrom305.99 and 306.67 mg EqGal/100g D.W, total flavonoid contents were within the range of 481.73-517.67 mgeqRE/100g D.W, and total anthocyanin contents were between 1.09 and 2.89 mg Eqcyanidin 3-glucoside/100g D.W. Antioxidant activity ranged from 17.18 to 18.11 mg EqTrolox/100g D.W for DPPH assayand from 27.97 to 29.60 mg EqTrolox/100f D.W for ABTS method.The highest values of total anthocyanin content and antioxidant activity (DPPH) were recorded in ʻBurlat’. The highest value of total flavonoid content was found in ʻCerisette’. Cherries from “Laanoceur”and “Toufselt” locations are characterized by similar biochemical composition and antioxidant activity, except for total anthocyanin content that shows slightly elevated values in “Laanoceur”. The close correlation between total phenolic contents and antioxidant activities (r2=0.73) show that antioxidant activity of cherry fruit depends on total polyphenols.


2020 ◽  
Author(s):  
Hao Zhou ◽  
Ke-Hai Zhou ◽  
Gang Zhao ◽  
Pei-Pei Wang ◽  
Dai-Gang Yang ◽  
...  

Abstract Backgroundcopper (Cu) is an essential micronutrient, required for plant growth and development. However, high concentrations of Cu can be extremely toxic to plant. This study investigate the tolerance mechanism of cotton under copper stress and its potential for soil pollution improvement.ResultsThe hybrid cotton variety (Zhongmian 63) and its two parent lines were selected as materials. Cotton seedling were treated with different Cu concentrations (0, 0.2, 50, 100, 200 μM) for 10 days in hydroponic condition. The results showed that the stem height, root length, and leaf area of cotton seedlings appear to have a down trend with the increase of Cu concentration. Increasing Cu concentration promoted Cu accumulation in roots, stems, and leaves of all the three cotton genotypes, however, the roots region was the main Cu storage organ, followed by leaves and stems regions. Compared with the parent lines, the roots of Zhongmian 63 are more capable of enriching Cu and have the least amount of Cu transported to the shoots. Therefore, the toxicity of Cu to cotton seedling is effectively alleviated. Cu-caused oxidative stress to cotton leaves was evident by over accumulation of H2O2 and MDA. POD activity and soluble sugar content increased firstly and then decreased compared with the control group. GSH content increased and photosynthetic pigment content decreased with increasing copper concentration in nutrient solution. ConclusionOur results suggest that the hybrid cotton variety Zhongmian 63 performed well under Cu stress. This lays the theoretical foundation for further analysis on molecular mechanism of cotton resistance to copper and promoting the large-scale planting Zhongmian 63 in the copper-contaminated area.


2019 ◽  
Vol 31 (11) ◽  
pp. 2517-2521
Author(s):  
Nguyen Quoc Duy ◽  
Huynh Anh Thoai ◽  
Tri Duc Lam ◽  
Xuan Tien Le

This study aims to investigate the variations in total phenolic content, total anthocyanin content, total flavonoid content and the antioxidant capacity of Roselle extracts in various extraction solvents. Extracts produced using three solvent systems (methanol, ethanol and acetone) at three different concentrations (50, 70 and 90 % (v/v)) were compared roselle calyx extract produced using distilled water. The antioxidant capacities of roselle calyx extracts were evaluated using DPPH free radical-scavenging capacity, ferric reducing antioxidant power (FRAP) and reducing power. The extraction efficiencies of phenolics, anthocyanins and flavonoids from roselle calyx varied considerably. The results showed that at 50 %, ethanol was the appropriate solvent for extraction of flavonoids, which achieved 508.64 mg RE/L and phenolics, which achieved 762.11 mg GAE/L, while at 70 %, methanol was the effective solvent for extracting anthocyanins, which achieved 8.404 mg/L. For antioxidant activity, at 50 % for ethanol, 70 % for methanol, 50 and 70 % for acetone were solvents used to obtain the highest DPPH free radical scavenging activities, ranging from 869.47-927.60 μmol TE/L. Thus, at 50 and 70 % for acetone were determined as solvents which gave extracts with the highest ferric reducing antioxidant power FRAP, ranging from 3493.52–3459.22 μmol TE/L.


Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 276 ◽  
Author(s):  
Stephen M. Boue ◽  
Kim Daigle ◽  
John C. Beaulieu ◽  
Mark Heiman

A low-cost method utilizing rice co-products to concentrate and stabilize blueberry polyphenols was developed that decreased the arsenic (As) content in rice bran. After concentration at 10 g/L, brown rice flour displayed a higher total anthocyanin content in both blueberry juice (2.7 mg/g) and pomace extract (2.6 mg/g) when compared to white rice flour. Defatted rice bran enriched with blueberry juice (10 g/L) had the highest concentration of polyphenols (16.0 mg/g), and defatted bran enriched with pomace extract had the highest concentration of anthocyanins (5.32 mg/g). Enriched rice flour and bran contained higher levels of anthocyanins when using pomace extracts. Polyphenols and anthocyanins were found to be highly stable at 37 °C in rice flour and bran samples combined with pomace extract. Polyphenol enrichment also produced lower total and inorganic arsenic (i-As) levels in defatted rice bran. Inorganic arsenic (i-As) concentrations in defatted rice bran enriched with blueberry juice and pomace extracts were reduced by 20.5% and 51.6%, respectively. Overall, rice flour and bran that are enriched with polyphenols and anthocyanins from blueberry pomace extracts are shelf and color stable, had low sugar content, and represent unique health-promoting food ingredients.


Author(s):  
Vishwanath Bidaramali ◽  
Shirin Akhtar ◽  
Anupam Das

Eggplant (Solanum melongena L.) is an important solanaceous crop of tropics and sub-tropics, having high production potential, and also a rich source of nutrients, particularly, carbohydrates, proteins, dietary fibre and vitamins like thiamin, niacin, pantothenic acid and folic acid, minerals like calcium, iron, potash, zinc, copper and manganese as well as bioactive compounds. However, eggplant is available in diverse shape and colour and consumer preference varies and the nutrient composition is different. In this investigation, we worked out the proximate nutrient compounds (on dry weight basis) and the bioactive compounds (on a fresh weight basis) in twenty diverse eggplant genotypes varying in colour and shape.  Moisture content in the fruits ranged from 71.54 to 91.36%, while carbohydrate content from 2.80 to 6.82%, crude protein 16.98 to 31.85%, nitrogen 2.49 to 4.35%, phosphorous 0.29 to 0.51%, potassium 1.65 to 4.54%, calcium 0.83 to 0.35%, iron 106.21 to 235.34 mg/kg, manganese 89.01 to 245.54 mg/kg, copper 18.73 to 98.56 mg/kg and zinc 60.73 to 75.77 mg/kg, each on dry weight basis. Biochemical parameters like total soluble solids (TSS) ranged from 1.27 to 3.94 °Brix, total sugar content 2.26 to 4.65%, ascorbic acid 0.66 to 3.53 mg/100 g, total anthocyanin content 0.35 to 18.85 mg/100 g FW, total chlorophyll 0.11 to 2.70 mg/100 g FW, total phenol 1.03 to 15.65 mg catechol equivalent/100 g FW, total antioxidant 1.16 to 2.26 µmol Trolox equivalent/g FW and radical scavenging activity 50.52 to 96.48%. Proximate nutrients were highest in Pusa Uttam, Pant Rituraj and BRBL-01, quality parameters in BRBL-07 and 71-19, while bioactive compounds were highest in Pant Rituraj, Pusa Purple Long, Pusa Purple Cluster and BRBL-01. These genotypes may be utilized in future breeding programmes for developing effective and nutritive cultivars.


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