scholarly journals Production of high-lysine-content biscuit and examination of the absorption of lysine in humans

2017 ◽  
Vol 10 (1) ◽  
pp. 21-35
Author(s):  
J. Prokisch ◽  
Z. Csiki ◽  
Cs. Albert ◽  
J. Csapó

AbstractIn the Medical and Health Centre of the University of Debrecen, we examined the changes in the free amino acid content of the blood serum of control and experimental individuals after consumption of 2,000 mg of lysine-laden biscuits. We baked the biscuits at 130 °C, during which the greater part (70–75%) of the lysine was not converted into Maillard reaction products. After 30–60 minutes of consumption of the biscuits, the free lysine content of the blood serum increased significantly in the experimental and control group with 41–46%, and even after three hours of consumption the level was 20% higher than in the initial concentration. The free arginine content of the blood serum did not change after the consumption of control and lysine biscuits neither in the control nor in the experimental group. Therefore, the free lysine/free arginine ratio of the individuals consuming lysine increased significantly compared to the initial and the control group’s value. The antioxidant level of the blood serum in the control group remained unchanged after the consumption of the control biscuit, while in the case of the experimental individuals who consumed lysine-fortified biscuits it increased by 40–45% compared to the initial level. Summing up: After consumption of the biscuits with 2,000 mg of free lysine, the concentration of free lysine in the blood serum, its free lysine/free arginine ratio and antioxidant level increased significantly. Our researches have clearly demonstrated that the active substances of the biscuit got into the blood serum, so the investigation of the active substance and the evaluation of the physiological effects are definitely recommended in the long run.

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 631-631
Author(s):  
Armando Gomez-Ojeda ◽  
Renata Elizarraraz-Morrill ◽  
Claudia Luevano-Contreras ◽  
Lorena del Rocío Ibarra-Reynoso ◽  
Jaime Uribarri ◽  
...  

Abstract Objectives Free fructose but not total fructose intake could contribute to the formation of advanced glycation end products (AGEs) and Maillard reaction products in the intestinal lumen. Therefore, the aim of this study was to compare serum levels of fructosamine, soluble receptor for AGEs (sRAGE), and urinary levels of glyoxal and methylglyoxal after free fructose and total fructose intake. Methods This is a randomized crossover study, approved by the Institutional Committee of Bioethics in Research of the University of Guanajuato (CIBIUG). Twelve clinically healthy subjects, 20–30 years old, were recruited. During the first visit, participants were randomly assigned to one of two treatments, an oral load of fructose (40 g) or total fructose (40 g fructose and 40 g glucose), in addition to their assigned treatment subjects received 25 g of hydrolyzed protein (Nature Best Isopure®) After a washing period (one week), participants were crossed over to receive the other treatment. Blood and urine samples were collected at baseline and after three hours of the oral load of each treatment. Glucose, creatinine, and lipids serum levels were measured with commercial kits. Insulin, fructosamine, and sRAGE serum levels were determined with ELISA commercial kits. Glyoxal and methylglyoxal urine levels were quantified by HPLC. Data analysis was completed using STATISTICA 10 and IBM SPSS Statistics 24.0 software. The results were considered significant with a value of P < 0.05. Results After free Fructose intake, participants had a significant decrease in sRAGE (1327 pg/L vs 1282 pg/L, P = 0.028), and in urine levels of glyoxal (0.63 ng/mg vs 0.24 ng/mg P = 0.028) and methylglyoxal (0.42 ng/mg vs 0.13 ng/mg P = 0.002). After Fructose and glucose intake, participants only had a significant decreased in serum sRAGE (1256 pg/L vs 1199 pg/L, P = 0.015). Fructosamine did not showed a difference after either intervention. Conclusions The consumption of free fructose decreased serum sRAGE levels, as well as urinary excretion of glyoxal and methylglyoxal. These results may suggest a possible in vivo formation of AGEs by consuming free fructose. Nevertheless, more studies are necessary to assess the formation of methylglyoxal derived AGEs. Funding Sources This project was supported by the University of Guanajuato through the Research and Postgraduate Support Department (DAIP).


2019 ◽  
Vol 13 (04) ◽  
pp. 574-580
Author(s):  
Khaled Khalaf ◽  
Zahra Seraj ◽  
Hesham Hussein ◽  
Mahmoud Mando

Abstract Objectives The aim of this study was to compare root dimensions (length and mesiodistal widths) between subjects with mild hypodontia and an age- and sex-matched control group. Materials and Methods Root dimension measurements of all permanent teeth excluding third molars were made on standardly taken orthopantomograms of 50 individuals (25 hypodontia and 25 controls) attending the University of Sharjah Dental Hospital. The length and two mesiodistal widths were measured for each fully formed root. The length of the root was measured digitally by drawing a line from the midpoint and bisecting the mesiodistal cemento-enamel junction (CEJ) of the tooth and extended to its apex. The mesiodistal widths of each root were measured at the cervical region and at half way of and perpendicular to the length of the root. Statistical Analysis Two sample t-tests were used to compare root dimension measurements between the hypodontia and control groups. Results There were no significant differences between genders with regard to root length or widths measurements, and therefore genders were combined for further analysis. Patients with hypodontia have significantly shorter root lengths than controls for the upper central incisors, upper canines, first premolars, and lower first molars (p < 0.05). Similarly, root width at the midpoint of the root was found to be less in hypodontia group than that in controls for the upper central incisors, lower first premolars, upper first molars, and all second premolars (p < 0.05). Similar pattern of differences was found with regard to the root width at the cervical region (p < 0.05). Conclusions Patients with hypodontia have shorter and narrower roots of the whole permanent dentition except the upper lateral incisors, lower incisors, lower canines, and all second molars when compared with controls. In effect, this may affect the orthodontic treatment planning and implant placement.


2015 ◽  
Vol 49 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Anita Fekonja ◽  
Andrej Cretnik ◽  
Danijel Zerdoner ◽  
Iztok Takac

Abstract Background. Ovarian cancer is usually diagnosed in an advanced stage and the present clinical and diagnostic molecular markers for early OC screening are insufficient. The aim of this study was to identify potential relationship between the hypodontia and epithelial ovarian cancer (EOC). Patients and methods. A retrospective study was conducted on 120 patients with EOC treated at the Department of Gynaecologic and Breast Oncology at the University Clinical Centre and 120 gynaecological healthy women (control group) of the same mean age. Women in both groups were reviewed for the presence of hypodontia and the patients with EOC also for clinicopathological characteristics of EOC according to hypodontia phenotype. Results. Hypodontia was diagnosed in 23 (19.2%) of patients with EOC and 8 (6.7%) controls (p = 0.004; odds ratio [OR] = 3.32; confidence interval [CI], 1.42-7.76). There was no statistically significant difference in patients with EOC with or without hypodontia regarding histological subtype (p = 0.220); they differed in regard to FIGO stage (p = 0.014; OR =3.26; CI, 1.23-8.64) and tumour differentiation grade (p = 0.042; OR = 3.1; CI, 1.01-9.53). Also, bilateral occurrence of EOC was more common than unilateral occurrence in women with hypodontia (p = 0.021; OR = 2.9; CI, 1.15-7.36). We also found statistically significant difference between the ovarian cancer group and control group in presence of other malignant tumours in subjects (p < 0.001). Conclusions. The results of the study suggest a statistical association between EOC and hypodontia phenotype. Hypodontia might serve as a risk factor for EOC detection.


2019 ◽  
pp. 69-72
Author(s):  
Olga Nikolaevna Polozyuk ◽  
Tatyana Ivanovna Lapina

The authors found out that the application  of dietary supplements "Glimalask Lact" allowed preventing the disorder of the gastrointestinal tract and saving all piglets throughout the experiment.  After application of "Agrocid super oligo", the safety of purebred piglets was 100 %, and for two pedigree piglets it was 96.7%.   In piglets of the 1st and 2nd control groups, disorder of the gastrointestinal tract was noted during the completion of colostral immunity and at weaning from sows.  The safety of purebred piglets was 96.7%, and of the two pedigree piglets  it was 93.4%. The number of γg-globulins in the blood serum of two breed piglings treated with the Glimalask Lact supplement increased by 3.5 and 2.5% compared to the piglets of the experimental group fed with Agrocid super oligo, and of the control group, The number of g-globulins in the blood serum of purebred  piglets it increased by 2.4 and 3.1% compared with analogues of the experimental and control groups.


2004 ◽  
pp. 43-47
Author(s):  
Róbert Szendi ◽  
Imre Bodó ◽  
Géza Nagy

In our experiment sows were grased during four grazing seasons, from April 28, 2000 to 23, August, 2001. The same number of indoor sows served as control animals.The results of the blood test show that, as a result of grazing, the beta carotene level of the blood serum has increased threefold. This difference disappeard after the farrow 30 days.On spring pasture, the grasingsows gained 50 kg in weight as opposed to 30 kg in the control animals. On the poor autumn pasture, the weight gained was only 30 kg, which was only 2 kg more in comparison with the performance of the control group. In the third grasing season the experiment sows weight gained was 13.7 kg and control group 37 kg. In the fourth season the control group weight gained was 4.4 kg more in comparison with the performance of the experiment sows.Grazing not very influence weight of gthe sows during the preast – feeding.


Konselor ◽  
2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Merikan Aren ◽  
Aruna Rauna Duamit

This study aims to determined the effects of Cognitive Behavioral Play Therapy (CBPT) techniques on low self-esteem among UNIMAS Students. More specifically, this study identified the effects of Cognitive Behavioral Play Therapy (CBPT) techniques in experimental group at pretest and posttest on low self-esteem among UNIMAS students and to identified the effects of Cognitive Behavioral Play Therapy (CBPT) techniques in control group at pretest and posttest on low self-esteem among UNIMAS students. A case study design is distributed questionnaire called as Rosenberg Self-Esteem Scale (RSES) and counselling sessions. Purposive sampling is used to get the most suitable respondents for this study. The findings of this study have revealed there were significant difference at pretest and posttest among respondents in experimental group and control group which were respondents in experimental group increased their level of self-esteem, while there were respondents decreased their level of self-esteem in control group. Counselling sessions utilizing Cognitive Behavioral Play Therapy (CBPT) techniques have increased the level of self-esteem among UNIMAS students in experimental group who attended the group counselling sessions because they have low level of self-esteem. All the dialogues that been stated by the client in the counselling sessions about their self-esteem can be related to respondents’ personality, cognitive, behavior, emotions and actions. The findings of this study have implications towards University students and counsellor. As for the university students, they gained insight, understanding and knowledge the importance of having good level of self-esteem. For the counsellor, counsellor being helpful, trusted and skillful which after utilized the CBPT techniques, they gained new skills and knowledge on that particular therapy.


Author(s):  
Leah Conejos Auxtero ◽  
Roar Abalos Callaman

Rubric has been associated with the term assessment used for grading and/or scoring. However, it might observe less, but it is also designed as students ‘learning tool. This study was conducted to provide empirical facts on its effectiveness as a learning tool in teaching Applications of Derivatives in Basic Calculus. It used the quasi-experimental design called the pretest posttest design. The participants were the 96 students from two classes of Grade 11 STEM students at the University of Mindanao. The instruments used were the adapted and improved rubric designed from two different research, a 25-item teacher-made problem-solving test questionnaire that was used in both pretest and posttest to measure the performance of the experimental and control group. The test questionnaire and rubric were both validated by 3 experts in the field with a result of very good, and it has a good internal consistency. The data gathered were summarized, translated, and analyzed using the mean scores of pretest and posttest. Findings showed that both the experimental and control group showed improvement, however, the experimental group who used rubric as a learning tool showed more significant improvement than control group. Thus, using a rubric as a learning tool in teaching Applications of derivatives is effective in improving students’ academic achievement as it teaches students to develop their understanding of procedural knowledge.


Author(s):  
Coco Marinella ◽  
Ramaci Tiziana ◽  
Viola Giuseppe ◽  
Guglielmino Antonino ◽  
Giulia Di Gregorio ◽  
...  

AbstractBackgroundMilton Erickson was the first to introduce hypnosis as a form of therapy during the post-war period. Numerous studies have evaluated the effects of hypnosis on memory, focusing principally on post-hypnotic amnesia, post-hypnotic hypermnesia, faux memories and learning growth.The aim of the present study was to determine if hypnosis can influence visual-spatial memory by increasing its performance and learning; to do this, we chose to utilise the Corsi Test (backward and forward).MethodsThree hundred second-year students enrolled in the psychology faculty at the University of Catania were informed of the research and its modalities, and an e-mail was sent to inquire if they wanted to participate in the experiment.Seventy female students took part in the research; 10 were excluded because they presented a high risk of being influenced under hypnosis.The 60 subjects in the research sample were randomly divided into two groups: the Experimental Group and Control Group.The protocol prescribed administration of the Corsi Test at Time 0 (start) followed by a resting phase of 30 min. The hypnotic state was subsequently introduced, and the Corsi Test was administered again.ResultsThe results of the Corsi Test for the Experimental Group showed statistically significant results (p<0.0004 and p<0.0001), while the results obtained in the Control Group did not show any significance.ConclusionsThese results led us to believe that hypnosis has the capacity to induce a nervous plasticity that supports learning of visual-spatial memory.


Author(s):  
Awatiful Azza ◽  
Cipto Susilo

Background and objective : Breastfeeding is one of the development tasks for women after childbirth. Many factors can affect mother’s success in breastfeeding exclusively, such as family support as well as local cultural influences. This research aims to apply the transcultural nursing model in Madura cultural perspective in breastfeeding mothers.Method: This research was conducted in Public Health Centre of Pakusari Suboh Jember. The data were collected using questionnaires, and focus group discuss. The research design was question-experimental research of post test design with control by using Paired Samples Test analysis. In addition, the researchers also performed a qualitative analysis to explore the Maduranese culture. The samples were breastfeeding mothers who had infants aged 1-6 months and 50 samples were then divided into two, i.e., treatment and control groups. Also, another data source were health professionals.Result: The result showed that the maternal age range between 15-34 years with an average age of 27 years. Besides, the average of the respondents’ education background was that 60% of them did not pass elementary school, both in treatment group and control group. The results of the analysis on cultural modifications was p-value of 0.001, which means that there is the effects of applying transcultural nursing model in improving milk production.Conclusion and recomendation : An application of cultural modifications that is able to increase milk production. Therefore, it needs a good cooperation for all the components of society in supporting breastfeeding mothers by modifying less the local culture that becomes  favorable for health.  


Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 241 ◽  
Author(s):  
Nesreen Aljahdali ◽  
Pascale Gadonna-Widehem ◽  
Pauline M. Anton ◽  
Franck Carbonero

Melanoidins are the final Maillard reaction products (protein–carbohydrate complexes) produced in food by prolonged and intense heating. We assessed the impact of the consumption of melanoidins from barley malts on gut microbiota. Seventy-five mice were assigned into five groups, where the control group consumed a non-melanoidin malt diet, and other groups received melanoidin-rich malts in increments of 25% up to 100% melanoidin malts. Feces were sampled at days 0, 1, 2, 3, 7, 14, and 21 and the microbiota was determined using V4 bacterial 16S rRNA amplicon sequencing and short-chain fatty acids (SCFA) by gas chromatography. Increased melanoidins was found to result in significantly divergent gut microbiota profiles and supported sustained SCFA production. The relative abundance of Dorea, Oscillibacter, and Alisitpes were decreased, while Lactobacillus, Parasutterella, Akkermansia, Bifidobacterium, and Barnesiella increased. Bifidobacterium spp. and Akkermansia spp. were significantly increased in mice consuming the highest melanoidin amounts, suggesting remarkable prebiotic potential.


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