scholarly journals Dynamics of change in broomrape populations (Orobanche cumana Wallr.) in Romania and Russia (Black Sea area)

Helia ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Dragan Škorić ◽  
Maria Joiţa-Păcureanu ◽  
Fyodor Gorbachenko ◽  
Oleg Gorbachenko ◽  
Stevan Maširević
Keyword(s):  
Crop Rotations ◽  
The Past ◽  
Oil Crops ◽  
Russian Research Institute ◽  
H Gene ◽  
Self Fertilization ◽  
Sunflower Broomrape ◽  
One Year ◽  
Sea Area ◽  
Russian Research

Abstract The emergence of new broomrape populations (races) has been observed in the past 20 years in several countries (Romania, Moldova, Ukraine, Russia, Turkey, Bulgaria, Spain, Serbia, and China) where sunflower is frequently grown in the same sites without applying traditional crop rotations. Differential lines for sunflower broomrape races A to F have been secured. The new broomrape races have been identified by researchers as races G and H. The question of whether the same broomrape mutations can occur in one year and affect the same countries remains unsolved. Several results of new broоmrape population emergences in some of the affected will be presented in the paper. A total of 390 genotypes were studied at four Romanian localities (Cuza Voda, Crucea-Stupina, Braila-Valea Canepii, and Tulcea-Agighiol) in 2014. At all four localities, a certain degree of sunflower broomrape infestation was observed in control hybrids and lines (Performer, LC-1093, LG-5661, and PR64LE20), which indicated the emergence of new populations higher than race H. The 390 studied genotypes had different reactions in all four localities. In 2015, 10 hybrids and controls were studied at five Romanian localities (Ciresu-Braila, Iazu-Ialomita, Stupina-Constanta, Topolog-Tulcea, and Viziru-Braila) and, according to the results, only hybrid Hy-7 was resistant in all localities. The results obtained from the three studied localities showed the emergence of new sunflower broomrape populations not controlled by gene for race H. Self-fertilization of hybrid Hy-7 produced the F2 generation in 2016. In 2017, broomrape resistance was studied at the infested (contaminated) plot at the All-Russian Research Institute of Oil Crops by the name of Pustovoit V.S. – VNIIMK in Rostov on Don. The plot was found to be infested by new broomrape populations originating from Russia, Ukraine, Romania, Turkey, and Spain. The obtained results showed an infestation degree in 17.1% plants of hybrid Hy-7, 35% in the F2 generation of Hy-7, control hybrids PR64LE25, LG-5580 and Donskoy-22 showed 19.4, 23, and 100% broomrape infestation, respectively. In conclusion, the plot contained broomrape populations which cannot be controlled by race H gene. According to the obtained results, a permanent change in variability of broomrape populations can be confirmed practically year after year. At present, new broomrape populations found at several localities are locally dispersed. Geneticists and breeders have to make joint efforts in further detailed studies of broomrape variability.

scholarly journals Breeding of sunflower lines resistant to race G of Broomrape

2021 ◽  
pp. 42-45
Author(s):  
Elena N. Ryzhenko ◽  
Nina M. Araslanova ◽  
Sergei V. Goncharov
Keyword(s):  
Oil Content ◽  
Parent Material ◽  
Sand Mixture ◽  
The Past ◽  
Oil Crops ◽  
Russian Research Institute ◽  
The Russian Federation ◽  
New Races ◽  
Russian Research

Relevance. Over the past decades, new and extremely virulent races have appeared in the southern regions of the Russian Federation, which overcame the resistance of domestic and foreign sunflower range of varieties. The most effective, long-term, and biologically safe way to protect sunflower from broomrape is to develop varieties and hybrids resistant to new races.Methods. We identified broomrape seeds of race G using internationally accepted differentiator lines: Record 1-3 (C), S-1358 (D), P-1380 (E), LC1093 and P96 (F). We used sunflower lines and hybrids of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops as a parent material. We carried out the evaluation of broomrape resistance by the method of A.Ya. Panchenko. To develop an infectious background, we introduced seeds of broomrape race G into boxes with a soil and sand mixture at the rate of 200 mg per 1 kg of the mixture, distributing them evenly. We grew sunflower plants at a temperature of 25-27 °С and a 16-hour photoperiod. In 25-30 days after the emergence if seedlings, we dug up plants and counted the broomrape specimens on their roots by a scale.Results. We developed sunflower lines resistant to races of broomrape from A to G. All of them are of branched form, with different lengths of the period from germination to flowering, they differ in height and oil content. The line L 82 is distinguished by a high content of oleic acid in oil — 91%. The lines have a high combinability. We obtained experimental hybrid combinations using these lines.

INFLUENCE OF REMOTE CHERRY HYBRIDS ON ITS GROWTH AND DEVELOPMENT IN THE GARDEN

1970 ◽  
pp. 31-33
Author(s):  
А. S. Lyakhova
Keyword(s):  
General Condition ◽  
Growth And Development ◽  
Shoot Length ◽  
Tree Planting ◽  
Fruit Crops ◽  
Russian Research Institute ◽  
One Year ◽  
The One ◽  
Experimental Garden ◽  
Russian Research

In the experimental garden of All-Russian Research Institute of Fruit Crops Breeding (VNIISPK) for 2017-2020 were studied scion-rootstock cherry combinations of the institute selection. Years of tree planting are 2013-2014, scheme is 5x2 m, density - 1000 trees / ha. The objective of the research is to assess the influence of distant cherry hybrids on its growth and development in the garden. The influence of rootstock forms Novella and Turgenevka plants varieties is presented. The general condition of cherry plants was good and varied from 4.2 to 5.0 points, the degree of flowering was at the level of the control variant. During the years of study, the indicator of the fruiting degree in the Novella variety was higher. The Novella variety trees on forms 74324, 74363, Ts-8-101 are medium-grown. Restrained growth of Turgenevka variety plants was observed in combinations with rootstock forms 74324 and 74340; medium-grown plants – 74322, 74332, 74336, 74363, Ts-8-1014; tall – growing – 82987. Plants of the Turgenevka variety in combinations 74324 and 74336 showed incompatibility, breaking off at the place of grafting concretion. The greatest value of the length of the one-year shoot was noted in Novella variety on the rootstock 74340, and the smaller one was in Ts-8-101. In cultivar Turgenevka a longer one-year shoot length was noted on forms 74332 and 74363, the smallest - 74336. The best rootstock forms among those studied in terms of growth and development for cultivar Novella are 74322, 74332.74340, 82987, Turgenevka – 74322, 82987.

scholarly journals The number of glandular trichomes on the peduncles of true lavender inflorescences as an additional breeding trait for essential oil

2021 ◽  
Vol 188 (4) ◽  
pp. 41-52
Author(s):  
S.V. Zelentsov ◽  
◽  
E.V. Moshnenko ◽  
T.P. Shuvaeva ◽  
I.V. Gaytotina ◽  
...  
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Significant Contribution ◽  
Distribution Density ◽  
Glandular Trichomes ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Average Size ◽  
Selection Of ◽  
Russian Research

The main receptacle of essential oil in true lavender is the peltate glandular trichomes of the calyxes in the whorls of the inflorescences. Their average size is 175 ± 25 µm, in some cases – up to 250 µm. For the extraction of lavender oil, not only the calyxes are used, but the whole inflorescences including the flowering shoots. The surface of the peduncles of lavender inflorescences is also covered with peltate glandular trichomes. However, their contribution to the total volume of essential oil in the inflorescence has almost never been determined. The aim of this research was to study the distribution density of glandular trichomes within the inflorescence and to determine the proportion of the contribution of flowering trichomes to the formation of essential oil in the inflorescence. The research was carried out in 2021 on the basis of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in two ecological and geographical points of the Krasnodar region. The object of the study was the true lavender varieties Voznesenskaya 34, Rannyaya, Yuzhanka and Voznesenskaya Aroma. It was found that the size of glandular trichomes on peduncles of true lavender is 90 ± 15 µm. Their number on peduncles, depending on the variety, varies from 2141 to 3003 pcs. The density of distribution of glandular trichomes on the surface of peduncles is equal to 8.60–14.93 pcs/mm3 . The total volume of essential oil in all glandular trichomes of peduncles is 0.41–0.57 cmm . The total volume of essential oil in the inflorescences varied from 2.28 to 5.15 cmm . The share of essential oil in the glandular trichomes of the peduncles in relation to the entire inflorescence ranged from 9.33 to 19.56%. It is concluded that peltate glandular trichomes on flower-bearing axes make a significant contribution to the essential oil content of lavender inflorescences. For the selection of true lavender to increase the essential oil content and the yield of essential oil, an additional selection trait is proposed – the amount of glandular trichomes on the surface of peduncles, which can be regulated by changing their distribution density on the surface of peduncles, or increasing the length of inflorescences.

scholarly journals The effect of herbicides on winter false flax (Camelina sativa (L.) Crantz.)

2021 ◽  
Vol 32 ◽  
pp. 02011
Author(s):  
Oksana Serdyuk ◽  
Victoria Trubina ◽  
Lyudmila Gorlova
Keyword(s):  
Oil Content ◽  
Plant Density ◽  
Central Zone ◽  
Application Rate ◽  
Oil Crops ◽  
False Flax ◽  
Russian Research Institute ◽  
Different Types ◽  
Flax Seeds ◽  
Russian Research

The purpose of the research was to determine the effect of herbicides on winter false flax and to identify the preparation that does not effect negatively on crop plants. The research was carried out in 20182020 at V.S. Pustovoit All-Russian Research Institute of Oil Crops. The experiment scheme included herbicides with active ingredients (a.i.), which effectively decreased the number of weeds on the plots. The effectiveness of the preparations was more than 70% for different types of weeds. However, the variants with the application of preparations with a.i. clopyralid 300 g/l, S-Metolachlor 960 g/l, ethametsulfuronmethyl 750 g/kg significantly decreased the plant density (by 18-32 pcs/m2) and seed yield (by 0.33-0.52 t/ha) of false flax in comparison with the control. The oil content of seeds was significantly decreased in the variants with the herbicides with a.i. S-Metolachlor 960 g/l with the application rate of 1.6 l/ha and ethametsulfuron-methyl 750 g/kg (by 1.2-1.5 %). In other variants, the oil content of false flax seeds differed from the control insignificantly (by 0.3-0.4 %). It has been established that the preparation with a.i. quinmerac 83 g/l + metazachlor 333 g/l with the application rate of 2.0 or 2.5 l/ha should be applied to decrease the number of weeds in the sowings of winter false flax in the central zone of the Krasnodar region. This preparation, without having a toxic effect, increases the yield by 0.15-0.17 t/ha and does not decrease the plant density and oil content of false flax seeds.

scholarly journals Efficiency of selection of self-fertile sunflower biotypes in production of foundation and breeder seeds

2021 ◽  
Vol 185 (1) ◽  
pp. 10-17
Author(s):  
A.D. Bochkovoy ◽  
◽  
V.I. Khatnyansky ◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
...  
Keyword(s):  
Oil Crops ◽  
Rostov Region ◽  
Russian Research Institute ◽  
Economically Valuable Traits ◽  
Breeder Seed ◽  
High Level ◽  
Efficiency Of Selection ◽  
Seed Growing ◽  
Selection Of ◽  
Russian Research

The researches were conducted on fields of OOO NPO “Triumph”, the Matveevo-Kurgansky district of Rostov region and V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar in 2017–2020. The purpose of the research was to develop methodology of selection of self-fertile sunflower genotypes in foundation and breeder seed production. Our work resulted in development of improved population Dobrynya plus. Middle self-fertility of this population at self-pollination was 13.7% vs. 5.2% of the initial variety (increased by 2.6 times), a ratio of the most valuable genotypes with amount of fully formed seeds more than 150 seeds per a plant increased from 13.5 to 26.4% (by 1.9 times). At the open flowering and free insect pollination, population Dobrynya plus does not differ by its economically valuable traits from the initial variety. But at the close flowering, without insects pollination, yield of the population Dobrynya plus was 0.93 t per ha vs. 0.52 t per ha of the initial variety (increase by 1.8 times). At selection of elite seeds from sunflower varietal populations for seed growing, the most perspective are the biotypes with high level of self-fertility which traits are held stable in the different environments. Improvement of sunflower variety by 11 self-fertility does not lead to decreasing of its yield qualities by the main economically valuable traits. The obtained experimental data prove a prospectivity of researches directed on improvement of sunflower variety populations by self-fertility at production of foundation and breeder seeds and possibility to reach successful results.

scholarly journals Breeding of winter rapeseed in VNIIMK: history and new results (review)

2021 ◽  
Vol 188 (4) ◽  
pp. 87-95
Author(s):  
E.B. Bochkaryova ◽  
◽  
L.A. Gorlova ◽  
E.A. Strelnikov ◽  
V.V. Serdyuk ◽  
...  
Keyword(s):  
Cold Resistance ◽  
The State ◽  
Turnip Rape ◽  
High Oleic ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Winter Rapeseed ◽  
Intraspecific Hybrids ◽  
Russian Research ◽  
Research Institute

Breeding of winter rapeseed in the V.S. Pustovoit All-Russian Research Institute of Oil Crops was started at the end of 60th of previous century with a selection of perspective winter forms from hybrids of spring brown mustard and winter rapeseed. Heterosis in intraspecific hybrids was studied. The best intraspecific hybrids exceeded the parental forms by 20–36% by seed yield, by 20–27% by green mass yield. A possibility to create intervarietal hybrids based on heterostyly was studied. Conditions for plants acclimation to increase cold resistance of winter rapeseed were studied; methods of selection for cold resistance were developed. In 1982, the development of rapeseed and turnip rape cultivars of ‘00’ type began. To create diversity of breeding germplasm, a great amount of samples from European counties that actively deals with rapeseed breeding were included into the work. In the V.S. Pustovoit All-Russian Research Institute of Oil Crops, the methods of estimation of oil and fodder protein quality were perfected, cultivars of rapeseed and turnip rape of ‘00’ type (e.g. erucic acid free and with low glucosinolate content) were developed. As a result of breeding for oil quality, high oleic cultivars were developed, that not only improve upon the oil nutritive efficiency but open new possibilities of its technical uses due to increasing oxi- and thermostability. The first Russian high oleic winter rapeseed cultivar Olivin was developed in the V.S. Pustovoit AllRussian Research Institute of Oil Crops and introduced in the State Variety Commission in 2019. The linear cultivars of winter rapeseed were developed by inbreeding, the first interlinear hybrids – using a CMS Ogura system. One of them named Debyut was introduced in the State variety trials 2020.

scholarly journals The new spring rapeseed variety Sibiryak 60

2021 ◽  
Vol 186 (2) ◽  
pp. 101-104
Author(s):  
G.N. Kuznetsova ◽  
◽  
R.S. Polyakova ◽  
Keyword(s):  
Seed Yield ◽  
Standard Variety ◽  
Economic Traits ◽  
Family Selection ◽  
Early Maturity ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Rapeseed Variety ◽  
Russian Research ◽  
Research Institute

In 2020, we submitted the spring rapeseed variety Sibiryak 60 bred at the Siberian Experimental Station (branch of V.S. Pustovoit All-Russian Research Institute of Oil Crops) to the State Commission of the Russian Federation on testing and protection of the selection achievements. We developed the variety by the method of multiple individual-family selection from the variety Avangard (All-Russian Research Institute of Rapeseed, Lipetsk). In 2012, there was identified an elite plant and further breeding work was carried out to improve the main economic traits: early maturity, productivity, oil content, resistance to lodging, seed falling and to the main pathogens. The general characteristics of the variety are high seed productivity and low content of glucosinolates in seeds. According to the results of the competitive variety trial (2018–2020), the spring rapeseed variety Sibiryak 60 exceeded the standard variety Granit in seed yield by 0.29 t/ha and in oil yield by 0.12 t/ha. In the conditions of the Krasnodar region, the variety Sibiryak 60 exceeded the standard variety Granit in seed yield by 0.31 t/ha, in the conditions of the Lipetsk region – by 0.30 t/ha. The new variety is meant to for cultivation for seeds in the Volgo-Vyatka (4), Middle Volga (7), Ural (9), West Siberian (10), and East Siberian (11) regions. The originator of the variety Sibiryak 60 is V.S. Pustovoit All-Russian Research Institute of Oil Crops.

scholarly journals Search and analysis of nuclear sequences in sunflower genome associated with genes of resistance to bromrape (Orobanche cumana Wallr.) races

2020 ◽  
Vol 183 (3) ◽  
pp. 11-19
Author(s):  
D.L. Savichenko ◽  
◽  
S.Z. Guchetl ◽  
A.V. Golovatskaya ◽  
◽  
...  
Keyword(s):  
Physical Map ◽  
Pcr Analysis ◽  
Sequence Characterized Amplified Region ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Parental Lines ◽  
Sunflower Genome ◽  
Simple Sequence ◽  
Selection Of ◽  
Russian Research

Studying of sunflower lines resistant to broomrape race G, bred in V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), Krasnodar showed the genetic control of resistance is monogenic with incomplete dominance. The purpose of the research was to seek and analyze molecular markers for the genes of resistance to broomrape race G using PCRmethods. In our research we used six developed in VNIIMK lines-donors resistant to broomrape race G: RGP1, RGP2, RGB, RGL1, RGL2, RGA, and susceptible lines VK 101, VK 678, VK 680. We extracted DNA from the leaves of young sunflower plants using STAB-buffer. For PCR-analysis we used 17 pairs of primers of two types: SCAR (sequence characterized amplified region) and SSR (simple sequence repeat). Conditions of amplification are as recommended by authors of markers with experimental selection of a temperature for primers hybridization. Within the research we balanced involved into work SSR- and 12 SCAR-loci using BLAST and a set of HanXRQr1.0. Due to these data we composed a physical map of loci location. After assessment of six resistant and three susceptible parental lines, seven markers demonstrated polymorphism by a length of locus DNA and two ones – by presence/absence of amplified fragment. For markers ORS 683 and ORS 1112 we observed a relation between loci polymorphism and susceptibility of parental lines to broomrape. This was certified with a presence of an allele of 364 n.p. length in ORS 683 and an absence of an allele of 375 n.p. length in ORS 1112. These markers were noted as primary ones for hybridological analysis in F1 и F2 with selected pairs for crossing. Additional markers will be selected individually using obtained data on loci polymorphism. Thus, as a result of the research we created a physical map for the further markers selection, found hybrid combinations and markers from the studied ones for hybridological analysis.

scholarly journals Plutella xylostella (L.) population control in sowings of spring rapeseed using pheromones

2022 ◽  
Vol 42 ◽  
pp. 02004
Author(s):  
Sergey Semerenko ◽  
Nadezhda Bushneva
Keyword(s):  
Plutella Xylostella ◽  
Mating Disruption ◽  
Population Control ◽  
Diamondback Moth ◽  
Instrumental Method ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Synthetic Sex Pheromone ◽  
Disruption Method ◽  
Russian Research

The diamondback moth Plutella xylostella (Linnaeus, 1758) is a common pest of rapeseed and other crops of Brassicaceae family. Annual yield losses and costs of pest control worldwide are estimated at $ 4-5 billion. The pest has an increased tendency to develop resistance to insecticides. The use of traps with synthetic sex pheromone is a modern instrumental method of monitoring P. xylostella. The use of the mating disruption method will effectively decrease pest numbers and reduce the application of insecticides. In 2017-2020, we researched the pheromone activity and evaluated the mating disruption method in the sowings of spring rapeseed at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) (Krasnodar). We established that P. xylostella males were caught in traps with all tested dispenser types. The pheromone showed the greatest activity on the foil-film dispenser (F). The mating disruption method effectively decreased P. xylostella population in rapeseed sowing; the disruption effect by the end of crop vegetation was high and reached 82.5 %.

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