scholarly journals Breeding of sunflower lines resistant to race G of Broomrape

2021 ◽  
pp. 42-45
Author(s):  
Elena N. Ryzhenko ◽  
Nina M. Araslanova ◽  
Sergei V. Goncharov

Relevance. Over the past decades, new and extremely virulent races have appeared in the southern regions of the Russian Federation, which overcame the resistance of domestic and foreign sunflower range of varieties. The most effective, long-term, and biologically safe way to protect sunflower from broomrape is to develop varieties and hybrids resistant to new races.Methods. We identified broomrape seeds of race G using internationally accepted differentiator lines: Record 1-3 (C), S-1358 (D), P-1380 (E), LC1093 and P96 (F). We used sunflower lines and hybrids of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops as a parent material. We carried out the evaluation of broomrape resistance by the method of A.Ya. Panchenko. To develop an infectious background, we introduced seeds of broomrape race G into boxes with a soil and sand mixture at the rate of 200 mg per 1 kg of the mixture, distributing them evenly. We grew sunflower plants at a temperature of 25-27 °С and a 16-hour photoperiod. In 25-30 days after the emergence if seedlings, we dug up plants and counted the broomrape specimens on their roots by a scale.Results. We developed sunflower lines resistant to races of broomrape from A to G. All of them are of branched form, with different lengths of the period from germination to flowering, they differ in height and oil content. The line L 82 is distinguished by a high content of oleic acid in oil — 91%. The lines have a high combinability. We obtained experimental hybrid combinations using these lines.

Author(s):  
S.V. Zelentsov ◽  
◽  
E.V. Moshnenko ◽  
T.P. Shuvaeva ◽  
I.V. Gaytotina ◽  
...  

The main receptacle of essential oil in true lavender is the peltate glandular trichomes of the calyxes in the whorls of the inflorescences. Their average size is 175 ± 25 µm, in some cases – up to 250 µm. For the extraction of lavender oil, not only the calyxes are used, but the whole inflorescences including the flowering shoots. The surface of the peduncles of lavender inflorescences is also covered with peltate glandular trichomes. However, their contribution to the total volume of essential oil in the inflorescence has almost never been determined. The aim of this research was to study the distribution density of glandular trichomes within the inflorescence and to determine the proportion of the contribution of flowering trichomes to the formation of essential oil in the inflorescence. The research was carried out in 2021 on the basis of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in two ecological and geographical points of the Krasnodar region. The object of the study was the true lavender varieties Voznesenskaya 34, Rannyaya, Yuzhanka and Voznesenskaya Aroma. It was found that the size of glandular trichomes on peduncles of true lavender is 90 ± 15 µm. Their number on peduncles, depending on the variety, varies from 2141 to 3003 pcs. The density of distribution of glandular trichomes on the surface of peduncles is equal to 8.60–14.93 pcs/mm3 . The total volume of essential oil in all glandular trichomes of peduncles is 0.41–0.57 cmm . The total volume of essential oil in the inflorescences varied from 2.28 to 5.15 cmm . The share of essential oil in the glandular trichomes of the peduncles in relation to the entire inflorescence ranged from 9.33 to 19.56%. It is concluded that peltate glandular trichomes on flower-bearing axes make a significant contribution to the essential oil content of lavender inflorescences. For the selection of true lavender to increase the essential oil content and the yield of essential oil, an additional selection trait is proposed – the amount of glandular trichomes on the surface of peduncles, which can be regulated by changing their distribution density on the surface of peduncles, or increasing the length of inflorescences.


2021 ◽  
Vol 32 ◽  
pp. 02011
Author(s):  
Oksana Serdyuk ◽  
Victoria Trubina ◽  
Lyudmila Gorlova

The purpose of the research was to determine the effect of herbicides on winter false flax and to identify the preparation that does not effect negatively on crop plants. The research was carried out in 20182020 at V.S. Pustovoit All-Russian Research Institute of Oil Crops. The experiment scheme included herbicides with active ingredients (a.i.), which effectively decreased the number of weeds on the plots. The effectiveness of the preparations was more than 70% for different types of weeds. However, the variants with the application of preparations with a.i. clopyralid 300 g/l, S-Metolachlor 960 g/l, ethametsulfuronmethyl 750 g/kg significantly decreased the plant density (by 18-32 pcs/m2) and seed yield (by 0.33-0.52 t/ha) of false flax in comparison with the control. The oil content of seeds was significantly decreased in the variants with the herbicides with a.i. S-Metolachlor 960 g/l with the application rate of 1.6 l/ha and ethametsulfuron-methyl 750 g/kg (by 1.2-1.5 %). In other variants, the oil content of false flax seeds differed from the control insignificantly (by 0.3-0.4 %). It has been established that the preparation with a.i. quinmerac 83 g/l + metazachlor 333 g/l with the application rate of 2.0 or 2.5 l/ha should be applied to decrease the number of weeds in the sowings of winter false flax in the central zone of the Krasnodar region. This preparation, without having a toxic effect, increases the yield by 0.15-0.17 t/ha and does not decrease the plant density and oil content of false flax seeds.


Author(s):  
S.V. Sklyarov ◽  
◽  
L.G. Ryabenko ◽  
V.S. Zelentsov ◽  
L.R. Ovcharova ◽  
...  

The researches were conducted in the laboratory of oil flax breeding of the V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar 2019– 2020. We studied the samples from the highly oleic collection of flax of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in order to select the best ones for introduction into a breeding process to improve the existing varieties and develop new ones with a good combination of biological and economically valuable traits. The samples from our collection demonstrated variability by the general economically valuable and biological traits. Thus, difference in duration of a period emergence–maturing was from 10 to 12 days depending on year and weather conditions. Maximal plant height (63.3 сm) was noted in 2020, and minimal (38.0 cm) was similar in all years of the research. Seed yield per a plot varied from 32.5 to 112.5 g/m 2 in 2019 and from 30.0 to 175.0 g/m 2 in 2020. The highest yield (175.0 g/m2) was fixed in 2020, and minimal one (30.0 g/m2) was at the same level in all years of the research. The samples from the collection were differed by their oil content in seeds: from 44.6 to 48.7% in 2019 and from 44.8 to 49.3% in 2020. Variation of this trait by years was 4.1–4.5%, difference between years of the research was equal to 0.4%. The presence of variability in oil contents in seeds among samples from flax collection proves a possibility to conduct selections by this trait in all breeding nurseries of the laboratory. Thus, in 2020 we selected two samples with oil content in seeds more than 49.0%.


2021 ◽  
Author(s):  
N.V. Ryabovol ◽  
◽  
A.A. Pikhtyaryova ◽  

The article is dedicated to the study of sulfonylurea-resistant population families of Dzhinnsur for their further use as a new breeding material. We carried out the experiments in field and laboratory conditions at V.S. Pustotovoit All-Russian Research Institute of Oil Crops (Krasnodar) in 2020. In the field experiment we treated the plants with the herbicide Express and we evaluated the plants by a scale of phytotoxicity in all population families of Dzhinn-sur. We the studied population families by the following characteristics: thousand-seed weight, huskness, oil content and linear size. As a result of this work, we selected four population families of Dzhinn-sur, which are constant in their herbicide resistance; they will be used in further breeding work.


2021 ◽  
Author(s):  
D.V. Starikova ◽  

One of the methods to improve initial germplasm by genetic way for development of lines which are stable by their economically valuable traits us inbreeding. Inbreeding allows disclosing a huge potential of inheritance of traits in variety. Impact of inbreeding on formation of yield, structural elements, oil quality and content has been noted by a range of scientists for many years. Using the inbreeding, researchers of the V.S. Pustovoit All-Russian Research Institute of Oil Crops developed rapeseed cultivars characterized with high yield, oil content and other valuable biochemical traits, requiring to the demands of agrarian and possessing industries.


Helia ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Dragan Škorić ◽  
Maria Joiţa-Păcureanu ◽  
Fyodor Gorbachenko ◽  
Oleg Gorbachenko ◽  
Stevan Maširević

Abstract The emergence of new broomrape populations (races) has been observed in the past 20 years in several countries (Romania, Moldova, Ukraine, Russia, Turkey, Bulgaria, Spain, Serbia, and China) where sunflower is frequently grown in the same sites without applying traditional crop rotations. Differential lines for sunflower broomrape races A to F have been secured. The new broomrape races have been identified by researchers as races G and H. The question of whether the same broomrape mutations can occur in one year and affect the same countries remains unsolved. Several results of new broоmrape population emergences in some of the affected will be presented in the paper. A total of 390 genotypes were studied at four Romanian localities (Cuza Voda, Crucea-Stupina, Braila-Valea Canepii, and Tulcea-Agighiol) in 2014. At all four localities, a certain degree of sunflower broomrape infestation was observed in control hybrids and lines (Performer, LC-1093, LG-5661, and PR64LE20), which indicated the emergence of new populations higher than race H. The 390 studied genotypes had different reactions in all four localities. In 2015, 10 hybrids and controls were studied at five Romanian localities (Ciresu-Braila, Iazu-Ialomita, Stupina-Constanta, Topolog-Tulcea, and Viziru-Braila) and, according to the results, only hybrid Hy-7 was resistant in all localities. The results obtained from the three studied localities showed the emergence of new sunflower broomrape populations not controlled by gene for race H. Self-fertilization of hybrid Hy-7 produced the F2 generation in 2016. In 2017, broomrape resistance was studied at the infested (contaminated) plot at the All-Russian Research Institute of Oil Crops by the name of Pustovoit V.S. – VNIIMK in Rostov on Don. The plot was found to be infested by new broomrape populations originating from Russia, Ukraine, Romania, Turkey, and Spain. The obtained results showed an infestation degree in 17.1% plants of hybrid Hy-7, 35% in the F2 generation of Hy-7, control hybrids PR64LE25, LG-5580 and Donskoy-22 showed 19.4, 23, and 100% broomrape infestation, respectively. In conclusion, the plot contained broomrape populations which cannot be controlled by race H gene. According to the obtained results, a permanent change in variability of broomrape populations can be confirmed practically year after year. At present, new broomrape populations found at several localities are locally dispersed. Geneticists and breeders have to make joint efforts in further detailed studies of broomrape variability.


Author(s):  
М. А. Dolzhikova ◽  
А. А. Pavlenko ◽  
А. V. Pikunova ◽  
O. D. Golyaeva

In the presented studies the genotypes of 46 varieties of red currant (Ribes rubrum) from the All-Russian Research Institute of Fruit Crops Breeding (VNIISPK) the collection were studied for 14 microsatellite locus. The data were obtained by the detection method of – fragment analysis using capillary electrophoresis. Varieties with rare, unique alleles and combinations of alleles have been identified. In most cases, no more than two fragments were identified for each genotype for a specific locus, but three fragments at some loci were amplified in the samples (Orlovchanka, Konstantinovskaya, Nadezhda – e3-B02; Marmeladnitsa – g2-G12; Nadezhda – g2-J08; Svetlitsa, Tatianina – g1-L12). The profiles of the cultivars Cascad and Red cross are identical to each other, although they differ from the others. The Red cross [(Cherry × White Grape] and Cascad [(free pollination of the variety Diploma (Cherry × White grape)]] varieties were developed in the USA and have common ancestors. It can be assumed the collection under different names contains the same genotype or that these pairs of varieties are so similar that the polymorphism of the analyzed loci is not enough to distinguish them. To prevent a shift in the size of alleles control varieties were selected and proposed, zoned in the territory of the Russian Federation – Gazelle (originator of VNIISPK: Chulkovskaya × Maarses Prominent) and Valentinovka (originator of VNIISPK: Rote Shpetlese × Jonker van Tets). The data obtained on the polymorphism microsatellite loci Red currant can be used for further identification.


Author(s):  
A.D. Bochkovoy ◽  
◽  
V.I. Khatnyansky ◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
...  

The researches were conducted on fields of OOO NPO “Triumph”, the Matveevo-Kurgansky district of Rostov region and V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar in 2017–2020. The purpose of the research was to develop methodology of selection of self-fertile sunflower genotypes in foundation and breeder seed production. Our work resulted in development of improved population Dobrynya plus. Middle self-fertility of this population at self-pollination was 13.7% vs. 5.2% of the initial variety (increased by 2.6 times), a ratio of the most valuable genotypes with amount of fully formed seeds more than 150 seeds per a plant increased from 13.5 to 26.4% (by 1.9 times). At the open flowering and free insect pollination, population Dobrynya plus does not differ by its economically valuable traits from the initial variety. But at the close flowering, without insects pollination, yield of the population Dobrynya plus was 0.93 t per ha vs. 0.52 t per ha of the initial variety (increase by 1.8 times). At selection of elite seeds from sunflower varietal populations for seed growing, the most perspective are the biotypes with high level of self-fertility which traits are held stable in the different environments. Improvement of sunflower variety by 11 self-fertility does not lead to decreasing of its yield qualities by the main economically valuable traits. The obtained experimental data prove a prospectivity of researches directed on improvement of sunflower variety populations by self-fertility at production of foundation and breeder seeds and possibility to reach successful results.


2021 ◽  
Vol 214 (11) ◽  
pp. 36-44
Author(s):  
Lyudmila Kudryavceva

Abstract. The purpose of the research is to characterize the varieties of long-legged flax included in the State Register of Breeding Achievements in terms of resistance to fusarium wilt, rust, anthracnose and pasmo. The research was conducted in 2018–2020 in vegetative, laboratory and field conditions at the All-Russian Research Institute of Flax (currently: A separate division Research Institute of Flax). As an object of research, we used varieties of flax-long-legged flax included in the State Register of Breeding Achievements of the Russian Federation for 2018–2020. Methods. The studies were carried out using modern mycological and phytopathological methods. Laboratory, vegetation and field experiments were conducted according to the All-Russian Research Institute of Flax methods. Results and practical significance. Among the studied 66 varieties, 56.1 % were highly resistant and resistant genotypes to fusarium wilt and 58.3 % – to rust. The specific weight of the Flax Institute varieties for this period was 34.8 %. High resistance to rust and fusarium wilt was characterized by the VNIIL selection varieties: Universal, Diplomat, Alexim, Dewdrop, Zaryanka, Aleksandrit. For the first time, scientists of the Flax Institute have created varieties of flax-long – legged (Diplomat and Tonus) resistant to three diseases: rust, fusarium wilt and anthracnose, and the Caesar variety-to four diseases. All varieties, with the exception of Diplomat and Tonus, a selection of the Flax Institute, were susceptible to anthracnose. The Grant variety of the selection of the Republic of Belarus and the Caesar variety were resistant to pasmo by 59.7 %, the rest were characterized by resistance to damage by 27.8–42.0 %. Scientific novelty. The characteristics of the resistance of the varieties included in the State Register of Breeding Achievements of the Russian Federation are comprehensively described on infectious and provocative backgrounds using natural and synthetic populations of pathogens diseases of flax. Varieties with group resistance to 2, 3, and 4 diseases are identified. The use of flax varieties resistant to the most economically dangerous diseases in flax crops will reduce the infectious potential and its accumulation in nature.


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