scholarly journals Pengaruh Konsentrasi dan Lama Perendaman GA3 terhadap Perkecambahan Benih dan Pertumbuhan Bibit Johar (Cassia seamea)

2021 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Ahmad Yunus ◽  
Arifiya Qifni ◽  
Puji Harsono ◽  
Bambang Pujiasmanto
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Gibberellic Acid ◽  
Active Compound ◽  
Root Length ◽  
Research Method ◽  
Percentage Germination ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Germination And Growth

Johar (<em>Cassia seamea</em>) is a medicinal plant with a cassiarin A compound to combat malaria and has a low Cassiarin A is an active compound in Johar (<em>Cassia seamea</em>) medicinal plant capable of combating malaria, despite showing a low seed production rate due to dormancy. This inactivity is severed using gibberellic acid (GA<sub>3</sub>) treatments. This study aims to determine the effects of concentration and soaking period of GA<sub>3</sub> on seed germination and growth of Johar seedlings. A completely randomized design (CRD), consisting of 2 treatments was applied as the research method. The first treatment employed GA<sub>3</sub> concentrations of 0, 45, 90 and 135 ppm, while the second was based on the soaking interval at 6, 12, 18 and 24 hours. The results showed the capacity of GA<sub>3</sub> able to shatter the seed dormancy with percentage germination of 43.03% and a concentration of 50.28 ppm. Therefore, the interaction of the two treatments significantly influenced the growth of Johar seedlings. Consequently, the concentration of GA<sub>3</sub> at 79.89 ppm increased the root length to 52.19 cm, while 84.68 ppm produced a total of 20.91 roots, after a 6 hour soaking. In addition, the 96.67 ppm expanded the stem diameter by 0.93 cm.

THE INFLUENCE OF THE EXTRACT OF SOME CROPS AND SOIL RESIDUES ON GERMINATION AND GROWTH

1960 ◽  
Vol 40 (1) ◽  
pp. 188-197 ◽  
Author(s):  
K. F. Nielsen ◽  
W. B. Woods ◽  
T. F. Cuddy
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Deionized Water ◽  
The Other ◽  
Percentage Germination ◽  
Soil Extracts ◽  
Field Crops ◽  
Time Required ◽  
Germination And Growth ◽  
Germinated Seeds

Six plant species were germinated in sand wetted with water extracts of tissues of five field crops and three soils. Deionized water was used as a check f he study was conducted using standard seed germination techniques.Alter 7 to 10 days all of the germinated seeds were harvested and measurements made of the shoot and root lengths.Alfalfa extract caused the greatest reduction in shoot and root length as well as m percentage germination. It caused the greatest increase in the time required for germination. Timothy extract was not quite as harmful as the alfalfa. Extracts of oats, corn and potatoes were still less harmful with potato extract causing the least effect.The soil extracts generally had very little effect when compared with deionized water.Plant species showed marked differences in tolerance to the extracts alfalfa being the most resistant and timothy the least.Where water alone was used rate of germination and per cent germination were as high or higher than with the other extracts, but the root and shoot lengths were not always the greatest.

scholarly journals Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus

2015 ◽  
Vol 61 (3) ◽  
pp. 13-22 ◽  
Author(s):  
Shamila Yadolahi Zadeh ◽  
Ali Akbar Ramin ◽  
Bahram Baninasab
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Salinity Stress ◽  
Echinacea Purpurea ◽  
Purple Coneflower ◽  
Randomized Design ◽  
Salinity Levels ◽  
The Mean ◽  
Completely Randomized Design ◽  
Mean Time

SummaryThis study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpureacv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1GA3with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1GA3for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3showed higher viability and better performance under salinity stress condition.

scholarly journals Bioregulators on seed germination and seedling growth of sweet basil

2020 ◽  
Vol 11 ◽  
pp. e3324
Author(s):  
Larissa Santos Castro ◽  
Danúbia Aparecida Costa Nobre ◽  
Daniel Andrés Villegas Hurtado ◽  
Willian Rodrigues Macedo
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Germination Rate ◽  
Ocimum Basilicum ◽  
Shoot Length ◽  
Naphthalene Acetic Acid ◽  
Initial Growth ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Completely Randomized Design

Basil (Ocimum basilicum L.) plants have multiple uses, ranging from spice to cosmetic purpose, besides being a source of essential oil and aromatic. These plants were commonly propagated by seed, however, few agrotechnologies are used to enhance the seed germination and initial growth in this crop. For this reason, our research aimed to evaluate aspects of seed germination of Ocimum basilicum L. cv. Limoncino, subjected to different bioregulators applied via substrate imbibition. The experiment was carried out in completely randomized design, with four treatments, as follows: T1: control (distilled water), T2: T13 a solution of a product formulated based on indolbutyric acid + naphthalene acetic acid + gibberellic acid (0.2 mL L-1, Liko Química), T3: gibberellic acid (0.5 mM L-1, progibb-400®) and T4: brassinosteroids (0.1 mM L-1). We evaluated: germination rate, germination speed index (GSI), first count, root and shoot length and chlorophyll a, b, total and carotenoids. The bioregulators did not influence germination, first count, GSI and photosynthetic leaf pigment, but application of gibberellic acid and brassinosteroids improve the shoot and root length, respectively. The gibberellic acid and brassinosteroids are promising bioregulators for stimulating initial basil growth.

scholarly journals Substrate and temperature on seed germination of Aspidosperma vargasii A. DC. (Apocynaceae)

2021 ◽  
Vol 17 (5) ◽  
pp. 66-72
Author(s):  
Izabele Domingues Soares Miranda ◽  
Manoela Mendes Duarte ◽  
Rodrigo Otávio Veiga de Miranda
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Relative Frequency ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Forest Species ◽  
Randomized Design ◽  
Mean Germination Time ◽  
Completely Randomized Design ◽  
Number Of Seeds

The Amazon has a wide diversity, including forest species with timber and non-timber potential, which often have their use restricted due to a lack of silvicultural information. Thus, the present study aimed to evaluate the effect of substrates and temperatures on seed germination of Aspidosperma vagarsii. Therefore, the premise of a completely randomized design was followed, in a 3 x 3 factorial scheme, with three substrates (paper, sand and vermiculite) and three temperatures (20, 25 and 30 °C). The germination percentage, germination speed index and mean germination time were determined, as well as germination relative frequency graphs. In addition, the weight of a thousand seeds, the number of seeds per kilo, and the moisture content was determined. The weight of thousand seeds was equal to 276.4 g, with 3,618 seeds per kilo, and a moisture content of 9.5%. There was no interaction between the studied factors. The sand substrate and temperatures of 20 and 25 °C presented the best results for seed germination of A. vargasii.

scholarly journals Vermicompost humates as a salinity mitigator in the germination of basil

2022 ◽  
Vol 52 (7) ◽  
Author(s):  
Juan José Reyes-Perez ◽  
Bernardo Murillo Amador ◽  
Luis Guillermo Hernández-Montiel ◽  
Pablo Preciado Rangel ◽  
Edgar Omar Rueda-Puente ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
Root Length ◽  
Dry Weight ◽  
Differential Response ◽  
Morphometric Characteristics ◽  
Shoot Height ◽  
Randomized Design ◽  
Tolerant Variety ◽  
Completely Randomized Design ◽  
Germination And Growth

ABSTRACT: This study determined the effect of vermicompost humates as salinity mitigator in germination and morphometric characteristics of basil varieties seedlings, tolerant and sensitive to salinity, subjected to vermicompost humates diluted 1/60 (v/v) and 0, 50 and 100 mM NaCl in a completely randomized design with four replications. The rate and percentage of germination, root length, shoot height, root and shoot fresh and dry-weight were measured. The varieties showed differential response, highlighting Napoletano as the most tolerant. Vermicompost humates showed biostimulant effects on variables evaluated, allowing that tolerant variety to improve germination and growth, and increasing the salinity tolerance of the sensitive variety.

scholarly journals Bioherbicidal Actions of Common Purslane on Seed Germination and Growth of Some Crop and Weed Species

2021 ◽  
Vol 910 (1) ◽  
pp. 012107
Author(s):  
Saber W. Hamad
Keyword(s):  
Seed Germination ◽  
Negative Impact ◽  
Growth Parameters ◽  
Dry Weight ◽  
Weed Species ◽  
Root Extracts ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Common Purslane ◽  
Germination And Growth

Abstract This study was conducted to examine allelopathic actions of Common Purslane Portulaca oleracea L. aqueous shoot and root extracts on germination of seeds and some growth parameters of wheat (Triticum aestivum) and rapeseed (Brassica napus). The experiment was performed in sterilized Petri dishes for one week at 22 °C. The experiment was arranged for completely randomized design. The concentrations were chosen for this study as (0%, 3%, 6% and 9%). The results indicated that the higher concentrations (6% and 9%) of both shoot and root extracts of common purslane caused significant reduction in seed germination whereas the lowest concentration caused the least negative impact on seed germination. Other growth polarimeters such as shoot and root length and shoot and root oven dry weight were significantly inhibited by the application of both Common Purslane aqueous shoot and root extracts at concentrations (6% and 9%) in wheat T. aestivum and rapeseed B. napus. However, dicot B. napus turned out to be more effected than monocot T. aestivum. The findings of this study suggest that common purslane aqueous shoot and root extracts can be recommended to utilize as bio-herbicide to supress seed germination and growth of weeds.

scholarly journals Aktivitas Ekstrak Metanol Daun Akasia (Acacia mangium Wild) Terhadap Perkecambahan Dan Pertumbuhan Maman Ungu (Cleome rutidospermaeD.C) Dan Rumput Grinting (Cynodon dactylon L. Pers)

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Sumi Riza linda Diah Wulandari Rousdy
Keyword(s):  
Plant Height ◽  
Root Length ◽  
Methanol Extract ◽  
Cynodon Dactylon ◽  
Acacia Mangium ◽  
Dry Weight ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Germination And Growth

Acacia (Acacia mangium Wild.) have alelokimia compound that can suppress the growth of the plants that it can be used as bioherbicide. This aimto study determined the concentration of the methanol extract of leaf of acacia capable of inhibit the germination and growth of weed, purple maman (Cleome rutidospermae DC.) and grass grinting(Cynodon dactylon L.). This study use a completely randomized design (CRD) with five replication and five treatment consist of control, concentrationmethanolextract0.2; 0.4; 0.6 and 0.8 g mlˉ1.Resultshowed that the methanol extract of leafacacia with a concentration of 0.4 g mlˉ1able to inhibit germinationpurple maman and grass grinting. Concentration of 0.2 g mlˉ1extract acacia can inhibit the growth of plant height, root length, and a decrease in wet weight and dry weight in weed purple maman andgrass grinting

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

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