Enzymatic-Assisted Microwave Extraction of Total Flavonoids from Bud of Chrysanthemum indicum L. and Evaluation of Biological Activities

2016 ◽  
Vol 12 (6) ◽  
pp. 607-613 ◽  
Author(s):  
Leqin Ke ◽  
Haiyan Chen
Keyword(s):  
Extraction Method ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Microwave Extraction ◽  
Water Heating ◽  
Factors Affecting ◽  
Chrysanthemum Indicum

Abstract An enzymatic-assisted microwave extraction method was developed to extract total flavonoids from bud of Chrysanthemum indicum L. The factors affecting total flavonoid yield were investigated and optimized by response surface design, and this extraction method was compared with water heating extraction. The antioxidant and antimicrobial activities of total flavonoids were preliminarily evaluated. The results showed that the optimized microwave extraction conditions were as follows: water–material ratio, 25:1 (mL:g); extraction time, 19 min; microwave power, 582 W. Under these conditions, the yield of total flavonoids was 11.21 ± 1.12 %, which was 53.21 % higher than water heating extraction method, and had shorter extraction time. The maximum scavenging capacity of total flavonoids (12 mg/mL) on DPPH, hydroxyl and superoxide anion free radical was 76.6 %, 78.8 % and 58.2 %, respectively. The total flavonoids showed inhibitory effect on Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae, with minimum inhibitory concentration of 8 mg/mL, 4 mg/mL, 4 mg/mL and 8 mg/mL, respectively. This study has provided the technical basis for further optimization of extraction of total flavonoids from bud of C. indicum L.

scholarly journals Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

2021 ◽  
Vol 12 ◽  
Author(s):  
Cíntia M. dos Santos ◽  
Leonardo M. de Souza Mesquita ◽  
Anna Rafaela C. Braga ◽  
Veridiana V. de Rosso
Keyword(s):  
High Performance ◽  
Salmonella Enteritidis ◽  
New Technologies ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Total Flavonoids ◽  
Future Studies ◽  
Operational Conditions ◽  
Total Antioxidant

Propolis is a resinous material rich in flavonoids and involved in several biological activities such as antimicrobial, fungicide, and antiparasitic functions. Conventionally, ethanolic solutions are used to obtain propolis phytochemicals, which restrict their use in some cultures. Given this, we developed an alcohol-free high-performance extractive approach to recover antibacterial and antioxidants phytochemicals from red propolis. Thus, aqueous-solutions of ionic liquids (IL) and eutectic solvents were used and then tested for their total flavonoids, antioxidant, and antimicrobial activities. The surface-responsive technique was applied regarding some variables, namely, the time of extraction, the number of extractions, and cavitation power (W), to optimize the process (in terms of higher yields of flavonoids and better antioxidant activity). After that, four extractions with the same biomass (repetitions) using 1-hexyl-3-methylimidazolium chloride [C6mim]Cl, under the operational conditions fixed at 3.3 min and 300 W, were able to recover 394.39 ± 36.30 mg RuE. g−1 of total flavonoids, with total antioxidant capacity evaluated up to 7595.77 ± 5.48 μmol TE. g−1dried biomass, besides inhibiting the growth of Staphylococcus aureus and Salmonella enteritidis bacteria (inhibition halo of 23.0 ± 1.0 and 15.7 ± 2.1, respectively). Aiming at the development of new technologies, the antimicrobial effect also presented by [C6mim]Cl may be appealing, and future studies are required to understand possible synergistic actions with propolis phytochemicals. Thereby, we successfully applied a completely alcohol-free method to obtain antimicrobials phytochemicals and highly antioxidants from red propolis, representing an optimized process to replace the conventional extracts produced until now.

scholarly journals Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

2020 ◽  
Vol 67 (4) ◽  
pp. 1250-1261
Author(s):  
Saniye Akyıl ◽  
Işıl İlter ◽  
Mehmet Koç ◽  
Zeliha Demirel ◽  
Ayşegül Erdoğan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Extraction Method ◽  
Phaeodactylum Tricornutum ◽  
Phenolic Content ◽  
Extraction Methods ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Extraction

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 μg/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 μg/mL extract) at an amplitude of 55.72%, a higher biomass/solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.

scholarly journals Biological Activities of Bellis perennis Volatiles and Extracts

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Neslihan Kavalcioğrlu ◽  
Leyla Açık ◽  
Fatih Demirci ◽  
Betül Demirci ◽  
Hülya Demir ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Plant Materials ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging

Different parts of Bellis perennis were subjected to hydrodistillation and the products were subsequently analyzed by GC and GC/MS. Aqueous and methanol extracts were prepared from the aerial parts. Antioxidant [1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, reducing activity, total antioxidant] and antimicrobial activity of the plant materials were studied. The aqueous extracts showed higher DPPH scavenging activity (85.8% at 102.5 μg/mL) than the methanol extract. Reducing power was also observed for both tested extracts, where the formation of linoleic acid peroxides was more for the aqueous extract than the methanol extract. Antimicrobial activities of all plant materials were evaluated at varying concentrations against a panel of pathogens, but without pronounced inhibitory effect.

scholarly journals Three New Isoprenylated Flavones from Artocarpus chama Stem and Their Bioactivities

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 3
Author(s):  
Sukanya Dej-adisai ◽  
Kedsaraporn Parndaeng ◽  
Chatchai Wattanapiromsakul ◽  
Jae Sung Hwang
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Strong Activity ◽  
Phytochemical Investigation ◽  
Cutibacterium Acnes ◽  
New Compounds ◽  
Screening Result

Phytochemical investigation of Artocarpus chama stem was performed by chromatographic techniques, resulting from the isolation and structure elucidation of three new compounds, namely 3′-farnesyl-apigenin (1), 3-(hydroxyprenyl) isoetin (2), and 3-prenyl-5,7,2′,5′-tetrahydroxy-4′-methoxyflavone (3), and five known compounds, namely homoeriodictyol (4), isocycloartobilo-xanthone (5), artocarpanone (6), naringenin (7), and artocarpin (8). From the screening result, A. chama extract showed a potent tyrosinase inhibitory effect. Ihe isolated compounds 1, 4 and 6 also exhibited tyrosinase inhibition with IC50 of 135.70, 52.18, and 38.78 µg/mL, respectively. Moreover, compounds 3, 4, 5, 6, and 8 showed strong activity against Staphylococcus epidermidis, S. aureus, methicillin-resistant S. aureus, and Cutibacterium acnes. This study is the first report on phytochemical investigation with new compounds and biological activities of A. chama. Skin infection can cause dark spots or hyperpigmentation. The isolated compounds that showed both anityrosinase and antimicrobial activities will be further studied in in vivo and clinical trials in order to develop treatment for hyperpigmentation, which is caused by infectious diseases by microorganisms.

scholarly journals Phytotoxic and Antimicrobial Activities of Teucrium polium and Thymus decussatus Essential Oils Extracted Using Hydrodistillation and Microwave-Assisted Techniques

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 716 ◽  
Author(s):  
Ibrahim Saleh ◽  
Ahmed Abd-ElGawad ◽  
Abd El-Nasser El Gendy ◽  
Abeer Abd El Aty ◽  
Tarik Mohamed ◽  
...  
Keyword(s):  
Essential Oils ◽  
Biological Activities ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Secondary Products ◽  
Main Compound ◽  
Microwave Assisted ◽  
Teucrium Polium ◽  
Main Components

Essential oils (EOs) have been described as promising eco-friendly secondary products of aromatic plants with several biological activities. The present study aimed to characterize the chemical composition and explore phytotoxic and antimicrobial activities of Teucrium polium and Thymus decussatus EOs extracted using hydrodistillation (HD) and microwave-assisted extraction (MAE) methods. Twenty-seven and twenty-eight compounds were identified from HD and MAE extracted EOs of T. polium, respectively. The oxygenated sesquiterpenes (57.68%) were characterized as the main components of the hydrodistilled EO with a prominence of 6-epi-shyobunol (33.00%), while sesquiterpene hydrocarbons (54.48%) were the main components of the MAE method, with a prominence of delta-cadinene (25.13%). Eighteen and nineteen compounds, were characterized in T. decussatus EOs extracted using HD and MAE methods, respectively, and oxygenated monoterpenes represented the main components of both EOs with carvacrol (94.40% and 75.91%, respectively) as the main compound. The EOs extracted using the MAE method were slightly more phytotoxic than those extracted using the HD method. The T. decussatus EO extracted using the MAE method showed a higher inhibitory effect than T. polium by 16-, 32-, and 24-fold, regarding seed germination, shoot, and root growth of lettuce, respectively. Moreover, EOs extracted by HD method showed a similar pattern with 16-, 28-, and 14-fold effects. Both T. decussatus EOs exhibited potent inhibitory effect against all tested bacteria with an inhibition zone of 34–39 mm and the lowest minimum inhibitory concentration (MIC) of 0.49, 0.98, and 1.95 μg/mL against Aspergillus niger, Escherichia coli, and Staphylococcus aureus, respectively. However, the EOs of T. polium showed weak antibacterial activity and no antifungal effect. Further studies are needed for the characterization of bioactive major compounds, either singular or synergistic, at field scale and to determine their modes of action and safety.

scholarly journals Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Julio César López-Romero ◽  
Humberto González-Ríos ◽  
Aida Peña-Ramos ◽  
Carlos Velazquez ◽  
Moises Navarro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Seasonal Effect ◽  
Microdilution Method ◽  
High Antioxidant Activity

This study shows the seasonal effect on the antioxidant, antiproliferative, and antimicrobial activities of L. glaucescens Kunth (LG) leaves extracts. Their antioxidant activity was evaluated through the DPPH, FRAP, and ORAC assays. Their phenolic content (PC) was determined by means of the Folin-Ciocalteu method, and the main phenolic compounds were identified through a HPLC-DAD analysis. Antiproliferative activity was determined by MTT assay against HeLa, LS 180, M12.C3.F6, and ARPE cell lines. Antimicrobial potential was evaluated against Staphylococcus aureus and Escherichia coli using a microdilution method. All the LG extracts presented high antioxidant activity and PC, with quercitrin and epicatechin being the most abundant. Antioxidant activity and PC were affected by the season; particularly autumn (ALGE) and summer (SULGE) extracts exhibited the highest values (p<0.05). All extracts presented moderate antiproliferative activity against the cell lines evaluated, HeLa being the most susceptible of them. However, ALGE and SULGE were the most active too. About antimicrobial activity, SULGE (MIC90<800 μg/mL; MIC50 < 400 μg/mL), and SLGE (MIC50 < 1000 μg/mL) showed a moderate inhibitory effect against S. aureus. These findings provide new information about the seasonal effect on the PC and biological properties of LG extracts. Clearly, antioxidant activity was the most important with respect to the other two.

scholarly journals Bioassay-Guided Fractionation and Antimicrobial Activities of Padina australis Extracts

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Vajihe Akbari ◽  
Fereshteh Safaiee ◽  
Afsaneh Yegdaneh
Keyword(s):  
Biological Activities ◽  
Agar Plate ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Plate Count ◽  
Disc Diffusion ◽  
E Coli ◽  
Bioassay Guided Fractionation ◽  
The Persian Gulf ◽  
Mic Value

Objectives: Marine seaweeds synthesize different metabolites with various biological activities, including antimicrobial effects. The main object of this study was to fractionate the seaweed Padina australis and analyze the antimicrobial activities of these fractions to isolate the bioactive compounds. Methods: Padina australis was collected from the Persian Gulf and extracted by maceration with methanol-ethyl acetate solvent. The extract was evaporated and partitioned by the Kupchan method to yield hexane, dichloromethane, water, and butanol partitions. Dichloromethane partition was further fractionated by MPLC, and antimicrobial activity of the crude extracts and all fractions were investigated using disc diffusion and agar plate count methods. The MIC values of hexane, dichloromethane, chloroform, and butanol partitions and all fractions were calculated. Results: Fraction numbers 11, 4, and 7 showed the highest inhibitory effect with MIC value of 87 µg/mL, 156 µg/mL, and 156 µg/mL against E. coli, P. aeruginosa, and S. aureus, respectively. Conclusions: This study reveals that different fractions of P. australis have antimicrobial activities against various microorganisms.

Synthesis, Antimicrobial Evaluation and Docking Study of Novel Thiosemicarbazone clubbed with 1,2,3-Triazoles

2020 ◽  
Vol 16 ◽  
Author(s):  
Adinath D. Badar ◽  
Shubham M. Sulakhe ◽  
Mahesh B. Muluk ◽  
Naziya N. M. A. Rehman ◽  
Prashant P. Dixit ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Biological Activities ◽  
Dna Gyrase ◽  
Docking Study ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Molecular Docking Study ◽  
Triazole Derivatives ◽  
Antibacterial And Antifungal

Background: Thiosemicarbazone, 1,2,3-triazole and their derivatives received great pharmaceutical importance due to their prominent biological activities. In the present study, the molecular hybrid thiosemicarbazone-1,2,3-triazoles derivatives were synthesized and screened for their antimicrobial activities. Methods: A series of thiosemicarbazone clubbed with 1,2,3-triazole derivatives were synthesized via click chemistry approach in good yields. The structures of synthesized compounds were assigned by their spectral data. The in vitro antimicrobial activity was performed by the agar well diffusion method. A molecular docking study was performed to identify the possible mode of action of synthesized derivatives. Results: The compounds 5d, 5h, 5i and 5k have exhibited excellent antimicrobial activities against both antibacterial and antifungal pathogens. The active thiosemicarbazone-1,2,3-triazole derivatives have shown excellent binding affinity towards DNA gyrase. Conclusion: The molecular hybrid thiosemicarbazone-1,2,3-triazole derivatives were synthesized. The newly synthesized compounds were evaluated for their antimicrobial activities. Few of the thiosemicarbazone-1,2,3-triazoles derivatives have exhibited good antimicrobial activities. They have been shown excellent binding affinity towards DNA gyrase.

scholarly journals Changes in Amino Acid Profiles and Bioactive Compounds of Thai Silk Cocoons as Affected by Water Extraction

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2033
Author(s):  
Chuleeporn Bungthong ◽  
Sirithon Siriamornpun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Silk Protein ◽  
Extraction Time ◽  
Antioxidant Power ◽  
Silk Cocoon ◽  
Amino Acid Profiles

Silk proteins have many advantageous components including proteins and pigments. The proteins—sericin and fibroin—have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Therefore, the objectives of this study were to find a suitable silk protein extraction method to obtain products with chemical and biological properties suitable as functional foods in two strains of Bombyx mori silk cocoon (Nangsew strains; yellow cocoon) and Samia ricini silk cocoon (Eri strains; white cocoon) extracted by water at 100 °C for 2, 4, 6 and 8 h. The results showed that Nangsew strains extracted for 6 h contained the highest amounts of protein, amino acids, total phenolics (TPC) and total flavonoids (TFC), plus DPPH radical-scavenging activity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP), anti-glycation, α-amylase and α-glucosidase inhibition. The longer extraction time produced higher concentrations of amino acids, contributing to sweet and umami tastes in both silk strains. It seemed that the bitterness decreased as the extraction time increased, resulting in improvements in the sweetness and umami of silk-protein extracts.

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