scholarly journals Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

2021 ◽  
Vol 12 ◽  
Author(s):  
Cíntia M. dos Santos ◽  
Leonardo M. de Souza Mesquita ◽  
Anna Rafaela C. Braga ◽  
Veridiana V. de Rosso
Keyword(s):  
High Performance ◽  
Salmonella Enteritidis ◽  
New Technologies ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Total Flavonoids ◽  
Future Studies ◽  
Operational Conditions ◽  
Total Antioxidant

Propolis is a resinous material rich in flavonoids and involved in several biological activities such as antimicrobial, fungicide, and antiparasitic functions. Conventionally, ethanolic solutions are used to obtain propolis phytochemicals, which restrict their use in some cultures. Given this, we developed an alcohol-free high-performance extractive approach to recover antibacterial and antioxidants phytochemicals from red propolis. Thus, aqueous-solutions of ionic liquids (IL) and eutectic solvents were used and then tested for their total flavonoids, antioxidant, and antimicrobial activities. The surface-responsive technique was applied regarding some variables, namely, the time of extraction, the number of extractions, and cavitation power (W), to optimize the process (in terms of higher yields of flavonoids and better antioxidant activity). After that, four extractions with the same biomass (repetitions) using 1-hexyl-3-methylimidazolium chloride [C6mim]Cl, under the operational conditions fixed at 3.3 min and 300 W, were able to recover 394.39 ± 36.30 mg RuE. g−1 of total flavonoids, with total antioxidant capacity evaluated up to 7595.77 ± 5.48 μmol TE. g−1dried biomass, besides inhibiting the growth of Staphylococcus aureus and Salmonella enteritidis bacteria (inhibition halo of 23.0 ± 1.0 and 15.7 ± 2.1, respectively). Aiming at the development of new technologies, the antimicrobial effect also presented by [C6mim]Cl may be appealing, and future studies are required to understand possible synergistic actions with propolis phytochemicals. Thereby, we successfully applied a completely alcohol-free method to obtain antimicrobials phytochemicals and highly antioxidants from red propolis, representing an optimized process to replace the conventional extracts produced until now.

scholarly journals Metabolomic Profiling and Antioxidant, Anticancer and Antimicrobial Activities of Hyphaene thebaica

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 266
Author(s):  
Ghada A. Taha ◽  
Ibrahim B. Abdel-Farid ◽  
Hassan A. Elgebaly ◽  
Usama A. Mahalel ◽  
Mohamed G. Sheded ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Methanolic Extracts ◽  
Carcinoma Cell Lines ◽  
Metabolomic Profiling ◽  
Total Antioxidant ◽  
Positive Correlations

This study investigated the metabolic content and biological activities of Hyphaene thebaica leaves, male parts and fruits methanolic extracts. The phytochemical constituents were determined, and multivariate data analysis discriminated the evaluated three parts into three groups according to the content of phenolics, flavonoids, flavonols, saponins, anthocyanins and tannins. High-performance liquid chromatography (HPLC) profiling of polyphenols revealed the dominance of catechins, rutin and apigenin-7-glucosides in leaves, protocatechuin, p-hydroxybenzoic, syringic, vanillic, rosmarinic, p-coumaric acids and chrysin in male parts, and chlorogenic acid in fruits. Leaves and male parts showed stronger free radical scavenging activity than the fruits. Positive correlations between total antioxidant capacity and carbohydrates, phenolics and flavonols were observed. The three extracts exhibited potent anti-cancer activity against liver and lung carcinoma cell lines. All extracts exhibited antibacterial activity, while only fruits showed antifungal efficacy. In conclusion, H. thebaica leaves, male parts and fruits contain a variety of phytochemicals with antioxidant, anticancer and antimicrobial activities.

scholarly journals Phytochemistry and Biological Activities of Amburana cearensis (Allemão) ACSm

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 505
Author(s):  
Zildene de Sousa Silveira ◽  
Nair Silva Macêdo ◽  
Suieny Rodrigues Bezerra ◽  
Abolghasem Siyadatpanah ◽  
Henrique Douglas Melo Coutinho ◽  
...  
Keyword(s):  
Chemical Composition ◽  
High Performance ◽  
Biological Activities ◽  
Literature Analysis ◽  
Future Studies ◽  
Analytical Technique ◽  
Popular Medicine ◽  
Brazilian Semiarid ◽  
Wide Range ◽  
Internet Searches

Amburana cearensis (Allemão) ACSm. belongs to the Fabaceae family and occurs in the Brazilian semiarid, Argentina, Paraguay, Bolivia, and Peru. Numerous studies that portray its ethnobotany, use in popular medicine, chemical composition, and biological activities exist in the literature. This review aimed to provide an overview of the chemical composition, ethnopharmacology, and biological activities associated with A. cearensis and its isolated constituents. Information was collected from internet searches in the Scopus, Medline, PubMed, Google Scholar, and ScienceDirect databases were performed covering publications from 1997–2020. An ethnopharmacological literature analysis revealed that A. cearensis is used to treat a wide range of respiratory disorders in addition to intestinal, circulatory, and inflammatory problems. Coumarins, flavonoids, phenolic glycosides, phenolic acids, phenylpropanoid derivatives, and triterpenoids, among others, have been reported as active compounds, with High-Performance Liquid Chromatography (HPLC) being the main analytical technique used. The A. cearensis extracts and compounds presented several biological activities, including antimicrobial, antinociceptive, anti-inflammatory, antioxidant, neuroprotective, and myorelaxant activities, among others. This review provides a useful bibliography for future investigations and A. cearensis applications; however, future studies should focus on its toxic effects and the mechanisms of action of its extracts and isolated constituents to guide clinical applications.

scholarly journals Biological Activities of Bellis perennis Volatiles and Extracts

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Neslihan Kavalcioğrlu ◽  
Leyla Açık ◽  
Fatih Demirci ◽  
Betül Demirci ◽  
Hülya Demir ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Plant Materials ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging

Different parts of Bellis perennis were subjected to hydrodistillation and the products were subsequently analyzed by GC and GC/MS. Aqueous and methanol extracts were prepared from the aerial parts. Antioxidant [1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, reducing activity, total antioxidant] and antimicrobial activity of the plant materials were studied. The aqueous extracts showed higher DPPH scavenging activity (85.8% at 102.5 μg/mL) than the methanol extract. Reducing power was also observed for both tested extracts, where the formation of linoleic acid peroxides was more for the aqueous extract than the methanol extract. Antimicrobial activities of all plant materials were evaluated at varying concentrations against a panel of pathogens, but without pronounced inhibitory effect.

scholarly journals Antimicrobial effect of a cyclic peptide Nostophycin isolated from wastewater cyanobacteria, Nostoc calcicola

2021 ◽  
pp. 94-101
Author(s):  
Vaishali Gupta ◽  
Deepak Vyas
Keyword(s):  
Antimicrobial Activity ◽  
Cyclic Peptide ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Fragmentation Pattern ◽  
Pharmaceutical Application ◽  
Freeze Dried ◽  
Nostoc Calcicola

Different types of peptides are produced by cyanobacteria of the genus Nostoc, which are unique in structure and have a wide spectrum of biological activities. The objective of the study to explore different habitats of organism and study antimicrobial activities to improve their pharmaceutical application and drug like properties by structure modification. A cyclic peptide nostophycin was isolated from Nostoc calcicola (MK506349) through freeze dried lyophilization method. Its structure has been elucidated with FT-IR, 1HNMR, 13CNMR and LC-MS. Glycine, d-glutamine, l-phenylamine, d-isoleucine, l-proline and a novel amino acid Ahoa are constituents of nostophycin. 1HNMR, 13CNMR spectroscopy confirmed the number of protons and carbons, and characteristics peak determined the structure and fragmentation pattern through LCMS.  Nostophycin possess Ahoa instead of Adha which makes it different from microcystin. Nostophycin exhibits antimicrobial activity against E.coli, S. aureus, C. albicens and A. niger. A good antifungal activity (9-52 μg/mL) and moderate  antimicrobial activity (concentration 18-52 μg/mL) were found for nostophycin. In case of already known peptides, these molecules may be further exploited to improve pharmaceutical application and future drug development.

scholarly journals Biological Activities of Tetrodotoxin-ProducingEnterococcus faeciumAD1 Isolated from Puffer Fishes

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Tu Hoang Khue Nguyen ◽  
Huu Ngoc Nguyen ◽  
Dat Van Nghe ◽  
Kim Hoang Nguyen
Keyword(s):  
Enterococcus Faecium ◽  
High Performance ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
16S Rrna Analysis ◽  
Sulforhodamine B ◽  
Biological Compounds ◽  
Pangasius Bocourti ◽  
Promising Source ◽  
Mrs Broth

Puffer fishes were collected from the central sea in Vietnam from spring to summer season. The eggs were incubated in MRS broth that was used to test the toxicity in mice and isolate the lactic acid bacteria community that could produce tetrodotoxin (TTX). Thin layer chromatography (TLC) and high performance lipid chromatography (HPLC) were used to detect and quantify TTX. As a result,Enterococcus faeciumAD1 which was identified by biochemical test and 16S rRNA analysis could produce TTX 0.3 mg/mL when cultured in MRS broth. The bacterium was optimized for TTX production and gave 0.18 mg/mL, 0.07 mg/mL, and 0.15 mg/mL in media prepared from the meat-washing water of freshwater fishes (Pangasius bocourti,Oreochromissp.) and sea fish (Auxis thazard), respectively, that are also hopeful to answer some poisoning cases related to eating fishes.Enterococcus faeciumalso showed the wide antimicrobial activities on yeast, Gram-negative and -positive bacteria. Extracted exopolysaccharide (EPS) that reacted with 2,2-diphenyl-1-picrylhydrazyl to give IC50at 5 mg/mL equaled 11 mg/mL ascorbic acid which could show effects on Hela-6 and Hep G2 using sulforhodamine B test.Enterococcus faeciumcan be claimed as a promising source in tetrodotoxin and biological compounds.

scholarly journals High-performance Liquid Chromatography Analysis and Antimicrobial Activities of Libyan Cistus salviifolius Extract

2021 ◽  
pp. 32-40
Author(s):  
Abdullah Alamami ◽  
Fatma Elshibani ◽  
Salmin Alshalmani ◽  
Mohamed Ali Sharkasi ◽  
Naema Elremali ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Cistus Salviifolius

Aims: This research is focused on the in vitro evaluation of Cistus salviifolius L. antimicrobial properties and the determination of the contents of phenols and flavonoids. Study Design: This research is analytical study aimed to illustrate the antimicrobial properties and to analyze the methanolic extract contents of aerial parts of Cistus salviifolius L. by high-performance liquid chromatography techniques. Duration: The study was performed within six months in the Faculty of Pharmacy, Benghazy University. Methodology: Antimicrobial properties was tested against twelve organisms using Kirby-Bauer disk diffusion sensitivity test and the determination of the contents of phenols and flavonoids was evaluated by running high-performance liquid chromatography techniques. Results: The findings indicated that catechin is the most abundant flavonoid in C. salviifolius, while gallic acid was the major phenol in the methanolic extract of the plant. The results also revealed that the methanol extracts had a significant antimicrobial potential particularly against Bacillus subtilis and Escherichia coli with MIC (0.98 and 0.49) µg/ml respectively, furthermore the extract was effective against Aspergillus fumigatus with MIC 0.98 µg/ml.       Conclusion: C. salviifolius was highly rich with flavonoids and phenols and has a significant antimicrobial effect.

scholarly journals Screening of Ten Tomato Varieties Processing Waste for Bioactive Components and Their Related Antioxidant and Antimicrobial Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 292 ◽  
Author(s):  
Katalin Szabo ◽  
Zorița Diaconeasa ◽  
Adriana-Florinela Cătoi ◽  
Dan Cristian Vodnar
Keyword(s):  
High Performance ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Total Carotenoids ◽  
Tomato Production ◽  
The Mean ◽  
Antioxidant And Antimicrobial Activities

Global tomato production is currently around 180 million tons, of which more than a quarter undergoes processing. The removed peels, seeds, and vascular tissues usually end up in landfills, creating environmental pollution. In order to highlight the alternative use of these vegetal wastes, our study investigated 10 tomato varieties in terms of carotenoids content, phenolic composition, and their related antioxidant and antimicrobial activities. Tomato peels extracts were screened by high performance liquid chromatography with diode-array detection (HPLC/DAD) for qualitative and quantitative analyses. The extracts were tested against six bacterial strains to determine their antimicrobial effect; the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was applied to estimate their antioxidant capacity. Total carotenoids content was significantly higher in Ţărănești roz, a local variety (5.31 ± 0.12 mg/100 g DW), while Mirsini, a commercial hybrid, presented significantly higher total phenolic content (155 ± 2 mg/100 g DW) compared to the mean value of all analyzed samples. The methanolic extracts of tomato peels presented acceptable antimicrobial activity against Staphilococcus aureus and Bacillus subtilis, and the mean antioxidant activity was 201 ± 44 µmol Trolox/100 g DW tomato peels. Considering that tomato peels have lycopene, β-carotene, lutein, and different phenolic compounds in their composition, tomato industrial by-products could represent a source of natural bioactive molecules with applicability in nutraceuticals and food industry.

Enzymatic-Assisted Microwave Extraction of Total Flavonoids from Bud of Chrysanthemum indicum L. and Evaluation of Biological Activities

2016 ◽  
Vol 12 (6) ◽  
pp. 607-613 ◽  
Author(s):  
Leqin Ke ◽  
Haiyan Chen
Keyword(s):  
Extraction Method ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Microwave Extraction ◽  
Water Heating ◽  
Factors Affecting ◽  
Chrysanthemum Indicum

Abstract An enzymatic-assisted microwave extraction method was developed to extract total flavonoids from bud of Chrysanthemum indicum L. The factors affecting total flavonoid yield were investigated and optimized by response surface design, and this extraction method was compared with water heating extraction. The antioxidant and antimicrobial activities of total flavonoids were preliminarily evaluated. The results showed that the optimized microwave extraction conditions were as follows: water–material ratio, 25:1 (mL:g); extraction time, 19 min; microwave power, 582 W. Under these conditions, the yield of total flavonoids was 11.21 ± 1.12 %, which was 53.21 % higher than water heating extraction method, and had shorter extraction time. The maximum scavenging capacity of total flavonoids (12 mg/mL) on DPPH, hydroxyl and superoxide anion free radical was 76.6 %, 78.8 % and 58.2 %, respectively. The total flavonoids showed inhibitory effect on Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae, with minimum inhibitory concentration of 8 mg/mL, 4 mg/mL, 4 mg/mL and 8 mg/mL, respectively. This study has provided the technical basis for further optimization of extraction of total flavonoids from bud of C. indicum L.

scholarly journals Characteristics, Phytochemical Analysis and Biological Activities of Extracts from Tunisian ChetouiOlea europaeaVariety

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ines Khlif ◽  
Karim Jellali ◽  
Thomas Michel ◽  
Maria Halabalaki ◽  
Alexios Leandros Skaltsounis ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Biological Activities ◽  
Antioxidant Property ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Polar Extracts ◽  
Layer Chromatography

This study selected 10 extracts from Tunisian chetouiO. europaeavariety for their total phenolics, flavonoids, and phytochemical analyses as well as for their antioxidant and antimicrobial activities determination. Thein vitroantioxidant property was investigated using DPPH, ferric reducing antioxidant capacity (FRAP), oxygen reducing antioxidant capacity (ORAC), andβ-carotene-linoleic acid bleaching assays while antimicrobial activity was evaluated using macrodilutions method. For all organs of chetouiO. europaeavariety, the investigated activities were found to be higher in the polar extracts (ethyl acetate, methanol, and methanol/water). These activities were correlated with the presence of phenolic compounds. Phytochemical analyses revealed that the crude extracts contain triterpenoids, quinones, and flavonoids. High performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) confirmed the presence of phenolic compounds in the studied extracts.

scholarly journals Influence of Extraction Solvent on the Phenolic Profile and Bioactivity of Two Achillea Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1601
Author(s):  
Dominika Kaczorová ◽  
Erna Karalija ◽  
Sabina Dahija ◽  
Renata Bešta-Gajević ◽  
Adisa Parić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phenolic Composition ◽  
Extraction Solvent ◽  
Ethanolic Extracts ◽  
Liquid Chromatography Tandem Mass

The phenolic composition, as well as the antioxidant and antimicrobial activities of two poorly investigated Achillea species, Achillea lingulata Waldst. and the endemic Achillea abrotanoides Vis., were studied. To obtain a more detailed phytochemical profile, four solvents with different polarities were used for the preparation of the plant extracts whose phenolic composition was analyzed using UHPLC-MS/MS (ultra-high performance liquid chromatography-tandem mass spectrometry). The results indicate that both of the investigated Achillea species are very rich in both phenolic acids and flavonoids, but that their profiles differ significantly. Chloroform extracts from both species had the highest yields and were the most chemically versatile. The majority of the examined extracts showed antimicrobial activity, while ethanolic extracts from both species were potent against all tested microorganisms. Furthermore, the antioxidant activity of the extracts was evaluated. It was found that the ethanolic extracts possessed the strongest antioxidant activities, although these extracts did not contain the highest amounts of detected phenolic compounds. In addition, several representatives of phenolic compounds were also assayed for these biological activities. Results suggest that ethanol is a sufficient solvent for the isolation of biologically active compounds from both Achillea species. Moreover, it was shown that the flavonoids naringenin and morin are mainly responsible for these antimicrobial activities, while caffeic, salicylic, chlorogenic, p-coumaric, p-hydroxybenzoic, and rosmarinic acid are responsible for the antioxidant activities of the Achillea extracts.

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