scholarly journals Protective efficacy of folic acid and vitamin B12 against nicotine-induced toxicity in pancreatic islets of the rat

2015 ◽  
Vol 8 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Ankita Bhattacharjee ◽  
Shilpi Kumari Prasad ◽  
Swagata Pal ◽  
Bithin Maji ◽  
Alak Kumar Syamal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Folic Acid ◽  
Vitamin B12 ◽  
Pancreatic Islets ◽  
Protective Efficacy ◽  
Fasting Blood Glucose ◽  
Pancreatic Tissue ◽  
Tissue Sections ◽  
Increased Risk

Abstract Although cigarette smoking is associated with insulin resistance and an increased risk for type 2 diabetes, few studies have examined the effect of nicotine on the adult endocrine pancreas. In this study, male Wister rats were treated with nicotine (3 mg/kg body weight/day) with or without supplementation of folic acid (36 μg/kg body weight/day) or vitamin B12 (0.63 μg/kg body weight/day) alone or in combination. Fasting blood glucose, insulin and HBA1C level and different oxidative and anti-oxidative stress parameters were measured and pancreatic tissue sections were stained with eosin-haematoxylene. Data were analysed by nonparametric statistics. The results revealed that nicotine induced prediabetes condition with subsequent damage to pancreatic islets in rats. Nicotine also caused oxidative stress in pancreatic tissue as evidenced by increased nitric oxide and malondialdehyde level and decreased superoxide dismutase, catalase and reduced glutathione level. Compared to vitamin B12 supplementation, folic acid blunted the nicotine-induced toxicity in pancreatic islets with higher efficacy. Further, folic acid and vitamin B12 in combination were able to confer significant protection on pancreatic islets against nicotine induced toxicity. These results suggest that supplementation of folic acid and vitamin B12 in combination may be a possible strategy of detoxification against nicotine-induced toxicity in pancreatic islets of the rat.

scholarly journals CURCUMIN PROTECTION AGAINST CADMIUM-INDUCED OXIDATIVE STRESS IN PANCREAS OF SWISS ALBINO MICE

2018 ◽  
Vol 11 (11) ◽  
pp. 393
Author(s):  
Suman Sharma ◽  
Anu Anu
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Protective Efficacy ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Treated Group ◽  
Positive Control ◽  
Pancreatic Tissue ◽  
Group 3 ◽  
Albino Mice

Objective: This study was planned to explore the protective efficacy of Curcuma longa (curcumin) against cadmium chloride (CdCl2)-induced oxidative stress in pancreatic tissue of albino mice.Methods: A total of 40 albino mice were equally divided into eight groups (five mice for each). The mice were kept on standard feed and water ad libitum. Control Groups 1 and 5 mice were given equal amount of distilled water, Groups 2 and 6 mice received 1 mg/kg body weight of CdCl2 on alternate days, and Group 3 and 7 received 1 mg/kg body weight of CdCl2 on alternate days and 100 mg/kg body weight of curcumin every day. Groups 4 and 8 received 100 mg/kg body weight of curcumin daily and were kept as positive control. Autopsies were done on 15 and 45 days post-treatment. The pancreas was collected from each mouse and homogenized for biochemical study.Results: The results of the present study revealed that Cd-induced oxidative stress depleted the antioxidant scavenger system and curcumin showed free radical scavenging potential. The activities of antioxidant enzymes, i.e., superoxide dismutase, catalase, glutathione peroxidase were observed to be significantly decreased, and methylenedioxyamphetamine concentration was increased in CdCl2-treated group which was restored to normal level after curcumin treatment.Conclusion: It can be concluded that curcumin acts as a powerful and beneficial antioxidant against oxidative stress by scavenging the free radicals produced by CdCl2.

scholarly journals Folic acid and vitamin B12 ameliorate nicotine-induced testicular toxicity in rats

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 353-368
Author(s):  
Dibyendu Ray ◽  
Ankita Bhattacharjee ◽  
Oly Banerjee ◽  
Shilpi Kumari Prasad ◽  
Siddhartha Singh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Folic Acid ◽  
Cigarette Smoking ◽  
Vitamin B12 ◽  
Inflammatory Cytokines ◽  
Reproductive Toxicity ◽  
Testicular Tissue ◽  
Albino Rats ◽  
Pro Inflammatory Cytokines

Introduction and Aim: Cigarette smoking, one of the fundamental roots of preventable morbidity, has a myriad of notorious effects. Nicotine is the most bountiful and symbolic constituent of cigarette smoke. The liaison between smoking and infertility has been investigated for decades; but it’s still dubious whether the noxious effects of cigarette smoking on testis and sperm characteristics are by virtue of nicotine. Therefore, the current study interrogated the ameliorative effects of folic acid and vitamin B12 on nicotine induced catastrophe in testicular tissue and sperm characters in male albino rats. Materials and Methods: Rats were treated with nicotine (3 mg/kg body weight/day, intraperitoneal) with or without folic acid (36µg/kg body weight/day, orally) and vitamin B12 (0.63µg/kg body weight/ day, orally) for 21 days. Sperm qualities were analyzed for motility and morphology. Various oxidative and anti- oxidative stress parameters, pro inflammatory cytokines levels, hormonal assays were performed. Results: Findings marked that nicotine caused degenerative changes in the testicular tissue. Supplementation with folic acid and vitamin B12 reversed these results along with suppressing the nicotine induced changes in TNF- ?, IL-6, and markers of oxidative stress. Moreover, folic acid and vitamin B12 in combination also significantly blunted the altered activities of testicular key androgenic enzymes, plasma levels of testosterone, LH, and FSH following nicotine exposure. Conclusion: In closure, testimonies manifested that folic acid and vitamin B12 may act as plausible strategy against oxidative stress, which is a pivotal step in nicotine-induced reproductive toxicity, and bettering functional status of testicular tissue by scavenging free radicals and hindering the generation of pro- inflammatory cytokines.  

scholarly journals Effect of the Total Extract of Averrhoacarambola (Oxalidaceae) Root on the Expression Levels of TLR4 and NF-κB in Streptozotocin-Induced Diabetic Mice

2015 ◽  
Vol 36 (6) ◽  
pp. 2307-2316 ◽  
Author(s):  
Xiaohui Xu ◽  
Tao Liang ◽  
Xing Lin ◽  
Qingwei Wen ◽  
Xingmei Liang ◽  
...  
Keyword(s):  
Body Weight ◽  
Histopathological Examination ◽  
Fasting Blood Glucose ◽  
Folk Medicine ◽  
Immunohistochemical Analysis ◽  
Tissue Expression ◽  
Pancreatic Tissue ◽  
Diabetic Mice ◽  
Total Extract ◽  
Expression Levels

Background: Averrhoacarambola L., which is a folk medicine used in diabetes mellitus (DM) in ancient China, has been reported to have anti-diabetic efficacy. Aims: The aim of this study was to evaluate the hypoglycemic effect of the extract of Averrhoacarambola L. root (EACR) on the regulation of the Toll-like receptor 4 (TLR4)-Nuclear-factor kappa B (NF-κB) pathway in B) pathway in streptozotocin (STZ)-induced diabetic mice. Methods: the mice were injected with STZ (120 mg/kg body weight) via a tail vein. After 72 h, the mice with FBG = 11.1 mmol/L were confirmed as having diabetes. Subsequently, the mice were treated intragastrically with EACR (300, 600, 1200 mg/kg body weight/d) and metformin (320 mg/kg body weight/d) for 14 days. Results: As a result the serum fasting blood glucose (FBG), interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-a) levels were decreased following EACR administration. Immunohistochemical analysis revealed that the pancreatic tissue expression levels of TLR4 and NF-κB were downregulated after EACR administration. EACR suppressed pancreatic mRNA expression level of TLR4 and blocked the downstream NF-κB pathway in the pancreas. According to Western blot analysis EACR suppressed pancreatic TLR4 and NF-κB protein expression levels. Histopathological examination of the pancreas showed that STZ-induced pancreas lesions were alleviated by the EACR treatment. Conclusion: These findings suggest that the modulation of the IL-6 and TNF-a inflammatory cytokines and the suppression of the TLR4-NF-κB pathway are most likely involved in the anti-hyperglycemic effect of EACR in STZ-induced diabetic mice.

scholarly journals ANTI-DIABETIC EFFECT OF ETHANOL EXTRACT OF Copaifera salikounda (HECKEL) AGAINST ALLOXAN-INDUCED DIABETES IN RATS

2021 ◽  
Vol 58 (2) ◽  
Author(s):  
Chinyere Aloke ◽  
Emmanuel Igwe ◽  
Nwogo Obasi ◽  
Pascal Amu ◽  
Egwu Ogbonnia
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Medicinal Plants ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
The Body ◽  
Albino Rats ◽  
Dependent Manner

Accumulating evidences have reinforced the use of medicinal plants in the treatment of various ailments as a result of negative side effects associated with conventional drugs. Plant components such as phenols and flavonoids with antioxidant potential have confirmed protective roles against oxidative stress-induced degenerative diseases like diabetes mellitus (DM). The current study was carried out to investigate the effect of seed pod ethanol extract from Copaifera salikounda (SPEECS) in alloxan-induced diabetic rats. SPEECS was obtained by maceration of seed pod powder in absolute ethanol for 72 h, filtered, concentrated and dried in-vacuo. Gas chromatography-mass spectrometry (GC–MS) technique was used to quantitatively elucidate the chemical constituents of SPEECS. Twenty-four male albino rats were randomly allocated into four groups (n=6): normal control, DM control, DM + 200 mg/kg SPEECS and DM + 400 mg/kg SPEECS groups. DM was induced in the Wistar albino rats through intraperitoneal injection of 200 mg/kg body weight of alloxan. After 14 days of treatment, the body weight changes and the fasting blood glucose level were determined in the different groups. Also, serum biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin (ALB), total protein (TP), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were estimated. The GC-MS results confirm nine bioactive compounds with 9-octadecenoic acid (55.75%) being most abundant. SPEECS (200 and 400 mg/kg) administration significantly (P 0.05) caused gain in weight, decreased fasting blood glucose and reversed the elevated liver function enzymes (ALT, AST, ALP) while total TP and ALB were markedly elevated relative to DM control group. Furthermore, SPEECS attenuated the activities of SOD and CAT while the level of MDA was significantly (P 0.05) decreased in dose dependent manner in comparison to the DM control. This study indicated that SPEECS can alleviate hyperglyceamia of DM. Key words: Copaifera salikounda; oxidative stress; medicinal plants; diabetes mellitus; phytochemicals; orthodox ANTIDIABETIČNI UČINEK EKSTRAKTA ETANOLA Copaifera salikounda (HECKEL) NA SLADKORNO BOLEZEN, SPROŽENO Z ALLOXAN-om, PRI PODGANAHIzvleček: Obstaja vedno več dokazov, ki poudarjajo uporabnost zdravilnih rastlin pri zdravljenju različnih bolezni, tudi zaradi različnih negativnih stranskih učinkov, povezanih s konvencionalnimi zdravili. Rastlinske sestavine kot so fenoli in flavonoidi z antioksidativnim potencialom, imajo po nekaterih raziskavah zaščitno vlogo pred degenerativnimi boleznimi, ki jih povzroča oksidativni stres, kot je sladkorna bolezen diabetes mellitus (DM). Študija je bila izvedena z namenom raziskovanja učinka etanolnega semenskega ekstrakta iz rastline Copaifera salikounda (SPEECS) pri podganah s sladkorno boleznijo, ki jo je povzročil alloxan. SPEECS je bil pridobljen z maceracijo praška semen v prahu v absolutnem etanolu 72 ur ter nadaljnjo filtracijo, koncentracijo in sušenjem v vakuumu. Za kvantitativno ugotavljanje kemijskih sestavin SPEECS je bila uporabljena tehnika plinske kromatografije in masne spektrometrije (GC-MS). Štiriindvajset samcev podgan Wistar je bilo naključno razporejenih v štiri skupine (n=6): normalna kontrola, kontrola DM, DM + 200 mg/kg SPEECS in DM + 400 mg/kg SPEECS. DM je bil pri podganah sprožen z intraperitonealno injekcijo 200 mg/kg telesne mase alloxana. Po 14 dneh zdravljenja so bile pri različnih skupinah določene spremembe telesne teže in nivo glukoze v krvi (na tešče). Poleg tega so avtorji raziskave izmerili še nekatere serumske biokemične parametre kot so ravni alaninske aminotransferaze (ALT), aspartatne aminotransferaze (AST), alkalne fosfataze (ALP), albumina (ALB), skupnih proteinov (TP), malondialdehida (MDA), superoksiddismutaze (SOD) in katalaze (CAT). Rezultati GC-MS so v izvlečku SPEECS pokazali devet bioaktivnih spojin, v katerih je največ 9-oktadecenojske kisline (55,75%). SPEECS (200 in 400 mg/kg) je povzročil znatno (P 0,05) povečanje telesne mase, znižanje glukoze v krvi na tešče in znižal raven encimov pokazateljev jetrne funkcije (ALT, AST, ALP), medtem ko je bila raven TP in ALB pri podganah, ki so prejemale SPEECS izrazito povišana v primerjavi z DM kontrolno skupino. Zdravljenje s  SPEECS je tudi oslabilo aktivnosti SOD in CAT, medtem ko se je raven MDA znatno zmanjšala (P 0,05) v primerjavi s kontrolno skupino DM. Ta študija je pokazala, da lahko SPEECS ublaži hiperglikemijo pri sladkorni bolezni pri podganah.Ključne besede: Copaifera salikounda; oksidativni stres; zdravilne rastline; sladkorna bolezen; fitokemikalije; ortodoksni

scholarly journals The Benefits of Fasting to Improve Health Conditions and to Prevent Cardiovascular Disease

Jurnal NERS ◽  
2020 ◽  
Vol 14 (3) ◽  
pp. 383
Author(s):  
Wahyu Sukma Samudera ◽  
Gracia Victoria Fernandez ◽  
Rahmatul Fitriyah ◽  
Hidayat Arifin ◽  
Shenda Maulina Wulandari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Cardiovascular Disease ◽  
At Risk ◽  
Body Weight ◽  
Endothelial Dysfunction ◽  
Fasting Blood Glucose ◽  
Abdominal Circumference ◽  
Oxidative Stress Marker ◽  
Amyloid A

Introduction: Fasting is defined as the voluntary abstinence from eating for variable time intervals and it has been associated with potential beneficial impacts on human health. The study was to review the benefits of fasting on cardiovascular health in humans with or without cardiovascular disease.Methods: The databases search was done using the keywords ‘fasting’ and ‘cardiovascular system’ using Scopus, Science Direct and ProQuest, limited to between 2013 and 2019 for publication year. A total of 3.619 articles were obtained and 15 articles involving experimental and non-experimental studies were used as the reference material.Results: The findings showed that in people who are healthy, fasting can reduce the inflammatory markers (IL-1 & IL-6, TNF-α), the oxidative stress marker (Malondialdehyde), body weight, abdominal circumference, fasting blood glucose, LDL, triglyceride and blood pressure. In people at risk or with cardiovascular disease, fasting can reduce body weight, body mass index, abdominal circumference, fat percentage, blood pressure, triglyceride, the biomarker of inflammation (serum amyloid A), the biomarker of oxidative stress (protein carbonyl), the biomarker of endothelial dysfunction (asymmetric dimethylarginine) and increase the vascular endothelial growth factor.Conclusion: Based on these findings, fasting can improve the health condition of people at risk or with cardiovascular disease by improving the risk factors such as blood pressure, overweight and endothelial dysfunction. In people who are healthy, fasting can be used for the prevention of cardiovascular disease by helping to maintain their weight, blood pressure, LDL and triglyceride within the normal limits.

Cadmium acts as a silent killer of liver by inducing oxidative stress and hepatocellular injury and a possible amelioration by vitamin B12 and folic acid in rat model

2019 ◽  
Vol 1 ◽  
pp. 105-117
Author(s):  
A. Banerjee ◽  
P. Nandi ◽  
C. Bhattacharya ◽  
Z. Kabir ◽  
S. Mukherjee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Folic Acid ◽  
Vitamin B12 ◽  
Rat Model ◽  
Male Rats ◽  
Albino Rats ◽  
Therapeutic Effects ◽  
Hepatocellular Injury ◽  
Male Adult ◽  
Wistar Albino Rats

<br/><b>Purpose:</b> To investigate the involvement of oxidative stress in Cadmium (Cd) induced alteration in the functional status of the liver. And to assess the efficacy of folic acid and vitamin B12 in preventing Cd-induced damage in the same. <br/><b>Materials and methods:</b> The experiment was carried out for four weeks. For the experiment, 25 healthy male adult Wistar albino rats were randomly selected and were divided into five equal groups and treated as control, treated with Cd, supplemented with vitamin B12 and folic acid and in the combination of these two. After 28 days the liver function enzymes and oxidative stress parameters were measured. <br/><b>Results:</b> Cd is the silent killer of the hepatic system through the induction of oxidative stress in male rats. From this investigation, it is evident that the folic acid+vitamin B12 possess significant hepatoprotective and antioxidant activity against Cd-induced hepatotoxicity in the rat model. In addition, results revealed that the folic acid alone and or in combination with vitamin B12 blunted the hepatotoxic effect significantly. <br/><b>Conclusions:</b> Based on results obtained, it can be concluded that folic acid and vitamin B12 offer a protective effect in Cd-induced oxidative stress associated with hepatocellular injury. Folic acid and vitamin B12 can be considered as a potent natural antioxidant which has the capacity to provide protection against Cd-induced oxidative stress in the liver in rats. However, to elucidate the exact mechanism of this modulatory effect and to examine its potential therapeutic effects further studies are essential.

scholarly journals ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF LYGODIUM MICROPHYLLUM IN ALLOXAN INDUCED DIABETIC RATS

2018 ◽  
Vol 10 (12) ◽  
pp. 75
Author(s):  
D. G. Syahidah Nadiah Binti Abdull Majid ◽  
Mohammad Iqbal
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Antioxidative Effects ◽  
And Oxidative Stress

Objective: The antihyperglycemic and antioxidative effects of L. microphyllum were evaluated by using in vivo methods in normal and alloxan induced diabetic rats.Methods: Diabetes was induced in Sprague Dawley rats by injecting alloxan through intravenous (i. v) at a dose of 100 mg/kg of body weight. Aqueous extract of L. microphyllum at different doses (400, 200 and 100 mg/kg of body weight) was administered orally (orogastric intubation) for 14 d. Blood glucose and oxidative stress markers were measured. Hematoxylin and eosin staining method were used to examine the pancreatic tissues.Results: At the 14 d interval, fasting blood glucose showed a reduction in serum glucose levels in animals pretreated with L. microphyllum compared with alloxan alone treated group. Oxidative stress was noticed in rat’s pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-S-transferase, and catalase activities. Malondialdehyde showed a significant increase compared to the normal saline-treated control group. Serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 d significantly prevented these alterations and attenuated alloxan-induced oxidative stress (P<0.05).Conclusion: The results of the present study indicated that the antihyperglycemic potential of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it is concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress.

Effect of maternal micronutrients (folic acid and vitamin B12) and omega 3 fatty acids on indices of brain oxidative stress in the offspring

2014 ◽  
Vol 36 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Suchitra Roy ◽  
Pratiksha Sable ◽  
Amrita Khaire ◽  
Karuna Randhir ◽  
Anvita Kale ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Folic Acid ◽  
Vitamin B12 ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Brain Oxidative Stress
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