Antioxidant and antiproliferative potentials of methanol extract of Xylopia aethiopica (Dunal) A. Rich in PC-3 and LNCaP cells

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Oluwatosin Adekunle Adaramoye ◽  
Bettina Erguen ◽  
Bianca Nitzsche ◽  
Michael Höpfner ◽  
Klaus Jung ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Liver Homogenate ◽  
Chorioallantoic Membrane ◽  
Oh Radicals ◽  
Dependent Manner ◽  
Lncap Cells ◽  
Concentration Dependent Manner ◽  
Benzene Sulfonic Acid ◽  
Acid Hydrate

AbstractBackground:Our previous studies showed that fruit methanol extract fromMethods:PC-3 and LNCaP cells were cultured and treated with MEXA (10, 50 and 100 μg/mL). The sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate (XTT) and lactate dehydrogenase (LDH) assays were used to evaluate cell viability and cytotoxicity, respectively. DNA fragmentation was determined by cell death detection ELISA plus, and angiogenesis was assessed by chicken chorioallantoic membrane (CAM) assay. The antioxidant activities of MEXA were determined by DPPH and hydroxyl (OH) radicals’ scavenging methods as well as through the inhibition of lipid peroxidation (LPO) in rats’ liver homogenate.Results:MEXA at 100, 250 and 500 μg/mL scavenged DPPH by 48%, 62%, 70% and OH radical by 39%, 58%, 67%, respectively. MEXA significantly (p<0.05) inhibited LPO in a concentration-dependent manner. In addition, MEXA had antiproliferative effects on PC-3 and LNCaP with ICConclusions:These findings suggest that MEXA induces antiproliferative activity in PCa cells through a mechanism that involves apoptosis. Therefore, MEXA may be a potential therapeutic agent for PCa.

scholarly journals In vitro antioxidant and anti-inflammatory activities of methanol extract of Vitellaria paradoxa seed (shea seed)

2021 ◽  
Vol 11 (3) ◽  
pp. 057-065
Author(s):  
Babafemi Tosin Ogunbiyi ◽  
Oluwaseyi Adegoke Adetunji ◽  
Olubunmi Esther Ogunbiyi ◽  
Gogonte Hezekiah Amah ◽  
Modupe Olusola Adetayo ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
Antioxidant Activities ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Standard Drug ◽  
Maximum Percentage ◽  
Total Antioxidant ◽  
Anti Inflammatory

Vitellaria paraodoxa (shea tree) is the source of shea seed from which the well-known shea butter is derived. The methanol extract of shea seed was evaluated for its anti-inflammatory and antioxidant activities using diclofenac sodium and ascorbic acid as standard respectively in in vitro methods. The anti-inflammatory activity was determined by inhibition of protein denaturation of bovine serum albumin (BSA) and erythrocyte membrane stabilization of human red blood cell. The antioxidant activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) assays. The results showed that methanol extract of V. paradoxa seed at different concentration protects the heat induced protein denaturation with the maximum percentage inhibition of 27% (IC50=303.0 µg/mL, p<0.05) at 500µg/mL compared to the standard drug which induced maximum inhibition of 45% (IC50=261.4 µg/mL, p<0.05) at 500 µg/mL and the control. The percentage inhibition of the methanol extract and standard drug in erythrocyte stabilization assay increased in a concentration dependent manner with maximum inhibitory activity of 96.9% (IC50=31.47 µg/mL, p<0.05) and 95% (IC50=33.89 µg/mL, p<0.05) at 2000 µg/ml respectively, which indicates that methanol extract stabilized erythrocyte membrane against hypotonic induced hemolysis in a blood sample better than the standard drug. The maximum percentage inhibition of methanol extract and standard drug in DPPH assay were found to be at 97% (IC50=8.95 µg/mL, p<0.05) and 98% (IC50=6.72 µg/mL, p<0.05) respectively at 100 µg/ml. The absorbance of the reductive capacities in FRAP assay indicates that the methanol extract has higher reducing potency in a concentration dependent manner. The methanol extract exhibited total antioxidant capacity of 0.25 ± 0.04 µg/(AAE/g) when compared to the standard drug 0.87 ± 0.03 µg/(AAE/g) at highest concentration of 100 µg/ml. For TBARS assay, low absorbance value indicate a high level of inhibition of lipid peroxidation. The maximum percentage inhibition of methanol extract was 97.5 % (IC50=51.79 µg/mL, p<0.05) and ascorbic acid was 99% (IC50=52.30 µg/mL, p<0.05) at concentration of 20 µg/ml. The assay indicates that the methanol extract has higher inhibiting potency in a reverse concentration dependent manner. In conclusion, V. paradoxa seed may possess strong anti-inflammatory and antioxidant activities which could constitute a potential source for development of new therapy.

scholarly journals Beneficial effects of methanolic extract of Indigofera linnaei Ali. on the inflammatory and nociceptive responses in rodent models

2016 ◽  
Vol 52 (1) ◽  
pp. 113-123
Author(s):  
Raju Senthil Kumar ◽  
Balasubramanian Rajkapoor ◽  
Perumal Perumal ◽  
Sekar Vinoth Kumar ◽  
Arunachalam Suba Geetha
Keyword(s):  
Methanol Extract ◽  
Latency Period ◽  
Dependent Manner ◽  
Rodent Models ◽  
Hot Plate ◽  
Concentration Dependent Manner ◽  
Standard Drug ◽  
Beneficial Effects ◽  
Nociceptive Responses

ABSTRACT Indigofera linnaei Ali. (Tamil Name: Cheppu Nerinjil) belongs to the family Fabaceae, used for the treatment of various ailments in the traditional system of medicine. In the present study, the beneficial effects of methanol extract of whole plant of I. linnaei (MEIL) were evaluated on inflammation and nociception responses in rodent models. In vitro nitric oxide (NO), lipoxygenase (LOX) and cyclooxygense (COX) inhibitory activities were also performed to understand the mode of action. MEIL at the dose of 200 & 400 mg/kg, p.o. significantly inhibited carrageenan induced rat paw volume and reduced the weight of granuloma in cotton pellet granuloma model. The results obtained were comparable with the standard drug aceclofenac. The anti-nociceptive effect of MEIL in mice was evaluated in hot plate and acetic acid induced writhing model. The plant extract significantly reduced the number of writhes and the analgesic effect was higher than that of the standard drug aspirin. However, the extract fails to increase the latency period in hot plate method suggesting that the extract produce nociception by peripheral activity. The extract produced inhibitory effect on NO, LOX and COX in concentration dependent manner. The extract exhibited pronounced and selective COX-2 inhibition. Altogether, these results suggested that the methanol extract of Indigofera linnaei could be considered as a potential anti-inflammatory and analgesic agent.

scholarly journals Assessment of Antioxidant and Antimicrobial Activities of Silver Nanoparticles Biosynthesized by Haplophyllum Obtusifolium

2020 ◽  
Vol 8 (2) ◽  
pp. 94-98
Author(s):  
Mohammad Reza Rezaei ◽  
Ali Es-haghi ◽  
Parichehreh Yaghmaei ◽  
Maryam Ghobeh
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antimicrobial Agents ◽  
Antioxidant Activities ◽  
Antimicrobial Activities ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
The North ◽  
Chamber Temperature ◽  
Pharmaceutical Industries

Background: Plants comprise great antioxidant sources as a result of their redox and biochemical components, which are rich in secondary metabolites such as phenolic acids, flavonoids, and other constituents. Haplophyllum obtusifolium from polygonaceae is widely used for preventing and managing diabetes. This study investigated the antibacterial and antioxidant activities of silver nanoparticles (AgNPs) biosynthesized by H. obtusifolium. Methods: The aerial parts of H. obtusifolium were gathered from the north of Khorasan Razavi province, Iran and desiccated at the chamber temperature. The shoots were powdered by grinding, 5 g of the powder was mixed with 250 mL of deionized water, and the resultant blend was then filtered. Bactericidal properties and antioxidant activity of the nanoparticles were assessed using disk diffusion and DPPH (2, 2-diphenyl-1-picrylhydrazyl) tests, respectively. Results: The results of this study showed that the biosynthesized nanoparticles exhibited antibacterial activity against a gram-negative (Klebsiella pneumoniae) bacterium, but they had no effects on gram-positive Staphylococcus epidermidis. Antioxidant test results showed that these nanoparticles were capable of eliminating DPPH radicals in a concentration-dependent manner so that a more potent antioxidant activity was seen in higher concentrations of the nanoparticles. Conclusion: Our results suggested that H. obtusifolium can be used as a key source of antioxidants/ antimicrobial agents in food and pharmaceutical industries.

Methanol Extract of Mongolian Iris bungei Maxim. Stimulates 3T3-L1 Adipocyte Differentiation

2021 ◽  
Vol 21 (7) ◽  
pp. 3943-3949
Author(s):  
Jaegoo Yeon ◽  
Sung-Suk Suh ◽  
Ui-Joung Youn ◽  
Badamtsetseg Bazarragchaa ◽  
Ganbold Enebish ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Fatty Acid Synthase ◽  
Molecular Mechanisms ◽  
Adipocyte Differentiation ◽  
Methanol Extract ◽  
Regulatory Element ◽  
Peroxisome Proliferator ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Peroxisome Proliferator Activated Receptor

Iris bungei Maxim. (IB), which is native to China and Mongolia, is used as a traditional medicine for conditions such as inflammation, cancer, and bacterial infections. However, the effects of Iris bungei Maxim. on adipocyte differentiation have not been studied. In the present study, we first demonstrated the molecular mechanisms underlying the adipogenic activity of the methanol extract of Mongolian I. bungei Maxim. (IB). IB significantly enhanced intracellular lipid accumulation and adipocyte differentiation in 3T3-L1 preadipocytes in a concentration-dependent manner. Moreover, IB markedly stimulated the expression of genes related to adipogenesis such as peroxisome proliferator-activated receptor γ, adiponectin, and aP2. In addition, we also observed that IB induces lipogenic genes such as fatty acid synthase, sterol regulatory element binding protein 1c, stearoyl-CoA desaturase, and acetyl-CoA carboxylase. Interestingly IB regulated adipocyte differentiation in both the early and middle stages. Taken together, these adipogenic and lipogenic effects of IB suggest its efficacy for the prevention and/or treatment of type 2 diabetes.

scholarly journals In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz

2011 ◽  
Vol 8 (3) ◽  
pp. 1451-1455
Author(s):  
Ramalingam Mahesh ◽  
Hyo Won Jung ◽  
Jun Hong Park ◽  
Yong-Ki Park
Keyword(s):  
Cell Viability ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Neuronal Cell ◽  
Ethyl Acetate Fraction ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Ostericum Koreanum

Ostericum koreanummaximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction ofO. koreanumroot was subjected toin vitroantioxidant activity with different methods for free radical scavenging activities. In addition, the cell viability and nitric oxide release assays were performed here for the first time in neuroblastoma (Neuro-2a) cell cultures. Among all the tested methods, the ethyl acetate fraction was expressed very active, exhibiting a good Trolox equivalent values and IC50, comparable to that of the commercial antioxidants, Trolox and ascorbic acid, respectively. The results showed that there was a reduction of cell viability by the fraction in a concentration dependent manner. These results suggest thatO. koreanumshows good antioxidant activitiesin vitroby inhibiting free radicals. These findings provide a rationale for thein vivotesting. Also, the major constituents behind the antioxidant mechanisms of this fraction warrant further study.

Compositions and Biological Activities of Essential Oils of Kadsura longepedunculata and Schisandra sphenanthera

2007 ◽  
Vol 35 (02) ◽  
pp. 353-364 ◽  
Author(s):  
Lei Song ◽  
Jie-Ying Ding ◽  
Cui Tang ◽  
Chun-Hua Yin
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Chemical Compositions ◽  
Dependent Manner ◽  
Schisandra Sphenanthera

The chemical compositions, antimicrobial activities, antioxidant activities and cytotoxicities of the essential oils isolated from the root of Kadsura longepedunculata Finet et Gagnep (KLREO) and the fruit of Schisandra sphenanthera Rehd. et Wills. (SSFEO) were investigated.The analyses of gas chromatography-mass spectrometry (GC-MS) showed that cadinane type compounds and their derivatives were rich in both oils (54.2% and 39.7%, respectively) and δ-cadinene was the major component of both oils (13.8% and 25.6%, respectively). The antimicrobial activities of both oils were evaluated against five microorganisms with the disc diffusion and the broth micro-dilution method. Results showed that Gram-positive bacteria were more sensitive to both oils than Gram-negative bacteria and the yeast. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oil of KLREO were lower than those of SSFEO, indicating that the former possessed slightly stronger antibacterial capability than the latter. The reducing power and lipid peroxidation assays were employed to study the potential antioxidant activities of both oils. Both oils remarkably decreased the content of malondialdehyde (MDA) in rat liver homogenate in a dose dependent manner. The antioxidant activities of KLREO appeared to be more potent than that of SSFEO. The oils of KLREO and SSFEO exhibited concentration-dependent cytotoxicities and were proved to be toxic to HepG2 cells with IC50 of 147 and 189 μg/ml, respectively.

scholarly journals Antioxidant and Antimicrobial Properties of Moringa oleifera Leaves and Pods Extracts in Pork Meatballs During Cold Storage

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Patcharee Prasajak ◽  
Phanida Renumarn ◽  
Wichien Sriwichai ◽  
Pakkawat Detchewa
Keyword(s):  
Aqueous Extract ◽  
Cold Storage ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Concentration Dependent Manner

Effects of M. oleifera leaves and pods extracts on physicochemical properties, free radical scavenging properties, antimicrobial activities and sensory attributes of pork meatballs were evaluated during cold storage at 4°C for 15 days. The preparation of pork meatballs was divided into eight treatments as control, 0.02% butylated hydroxytoluene (BHT), 0.2% leaves and pods aqueous extract, 0.4% leaves and pods aqueous extract, 0.8% leaves and pods aqueous extract. Aqueous leaves extract showed highest level of total phenolic (67.18 mg GAE/g extract) and flavonoid contents (5.60 mg CE/g extract) compared to those observed in aqueous pods extract as 55.17 mg GAE/g extract and 3.54 mg CE/g extract, respectively. The leaves extract had strongest antioxidant activity against DPPH radicals with IC50 49.85 μg/ml while the pods extract exhibited IC50 99.31 μg/ml. According to pork meatballs analysis, meatballs samples with addition of aqueous leaves extract exerted higher antioxidant activities in a concentration-dependent manner that were performed by higher DPPH scavenging activity and lower TBARs values in comparison with aqueous pods extract. Conversely, M.oleifera pods extract showed highest antibacterial activity against all testedfoodborne bacterial strains including Staphylococcus aureus (TISTR 1466),Bacillus cereus (TISTR 678), Escherichia coli (TISTR 780), Salmonellatyphimurium (ATCC 13311) with lowest MIC (1.56 mg/ml) and MBC (3.13 mg/ml)in agreement with the decrease of total microbial counts as compared to controland BHT samples. The meatballs with pods extract possessed higher sensoryattributes scores than those added with the leaves extract. In conclusion, 0.8%pods extracts effectively retarded lipid oxidation as well as decreased microbialgrowth in pork meatballs during cold storage. However, it was point out thatinferior sensory scores were affected by increasing additional the extract in themeatballs. Therefore, the use of Moringa extracts should be carefully applied inthe meatballs for avoidance of lowering consumer acceptance.

scholarly journals Physicochemical, Structural, and Biological Properties of Polysaccharides from Dandelion

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1485 ◽  
Author(s):  
Huijing Guo ◽  
Weida Zhang ◽  
Ying Jiang ◽  
Hai Wang ◽  
Guogang Chen ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Effects ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Perennial Herb ◽  
Dependent Manner ◽  
Glycosidic Linkage ◽  
Food Ingredient ◽  
Concentration Dependent Manner ◽  
Ic50 Values

The edible and medicinal perennial herb dandelion is known to have antitumor, antioxidant, and anticomplement properties. However, the structural characterization and biological effects of its polysaccharides are not well understood. Here, we aimed to extract and investigate a novel polysaccharide from dandelion. A water-soluble polysaccharide, PD1-1, was successfully obtained from dandelion through ultrasonic-assisted extraction and purification using diethylaminoethyl (DEAE)–Sepharose fast flow and Sephadex G-75 columns. The results showed that PD1-1 is an inulin-type polysaccharide with a molecular weight of 2.6 kDa and is composed of glucose (52.39%), and mannose (45.41%). Glycosidic linkage analysis demonstrated that PD1-1 contains terminal α-d-Man/Glcp-(1→ and →1)-β-d-Man/Glcf-(2→ glycosidic linkage conformations. A physicochemical analysis indicated that PD1-1 has a triple helix structure and exhibits important properties, including good swelling, water-holding, and oil-holding capacities. Furthermore, PD1-1 showed good antioxidant activities in DPPH and hydroxyl free radical scavenging abilities, with IC50 values of 0.23 mg/mL and 0.25 mg/mL, respectively, and good hypoglycemic activities in α-amylase and α-glucosidase inhibition, with IC50 values of 0.53 mg/mL and 0.40 mg/mL, respectively, in a concentration-dependent manner. Results suggest that PD1-1 possesses efficacious antioxidant and hypoglycemic properties and has potential applications as a functional food ingredient.

scholarly journals Korean Propolis Suppresses Angiogenesis through Inhibition of Tube Formation and Endothelial Cell Proliferation

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Seon-Il Park ◽  
Toshiro Ohta ◽  
Shigenori Kumazawa ◽  
Mira Jun ◽  
Mok-Ryeon Ahn
Keyword(s):  
Umbilical Vein ◽  
Chorioallantoic Membrane ◽  
Tube Formation ◽  
In Vitro Models ◽  
Endothelial Cell Proliferation ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Korean Propolis

Propolis, a sticky material that honeybees collect from living plants, has been used for its pharmaceutical properties since ancient times. In this study, we examined the effects of ethanol extracts of Korean propolis (EEKP) from various geographic regions on the inhibition of angiogenesis, both in vitro and in vivo. The effects of EEKP were tested on in vitro models of angiogenesis, that is, tube formation and proliferation of human umbilical vein endothelial cells (HUVECs). All EEKP samples exhibited significant inhibitory effects on tube formation of HUVECs in a concentration-dependent manner (6.25-25 μg/mL). In addition, two EEKP samples, prepared from Uijeongbu and Pyoseon propolis, significantly suppressed the proliferation of HUVECs in a concentration-dependent manner (3.13-25 μg/mL). Furthermore, in an in vivo angiogenesis assay using the chick embryo chorioallantoic membrane (CAM) system, we found that the two EEKP samples significantly reduced the number of newly formed vessels. These results indicate that Korean propolis may have potential applications in the prevention and treatment of angiogenesis-related diseases such as cancer.

scholarly journals Phytochemical Screening and Antioxidant Potentials of Methanol Extract of Duranta erecta Leaves

2020 ◽  
pp. 16-26
Author(s):  
Christopher Chidiebere Ugwu ◽  
Chioma Assumpta Anosike
Keyword(s):  
Nitric Oxide ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Antioxidant Vitamins ◽  
Composition Analysis ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Antioxidant Power

Plant extracts that have antioxidant activities show promising effect in the management of many diseases initiated by oxidative stress. The present study evaluates the phytochemical composition and in vitro antioxidant potentials of methanol extract of Duranta erecta leaves. The extract was subjected to phytochemical screening, DPPH inhibition, ferric reducing antioxidant power (FRAP), nitric oxide determination, antioxidant vitamins and minerals composition analysis. All analyses were carried out using standard biochemical methods. The phytochemical evaluation of the plant extract showed the presence of flavonoids (24.20 ± 0.14 mg QE/g), Alkaloids (15.87 ± 1.71 mg/g), total phenol (12.73 ± 0.61 mg GAE/g), tannins (9.24 ± 0.03 mg TAE/g), terpenoids (8.90 ± 0.96 mg/g), steroids (2.65 ± 0.55 mg/g) and saponins (5.55 ± 0.76 mg/g). The in vitro antioxidant determination showed that the extract had antioxidant properties in a concentration dependent manner. The antioxidant property of the sample was compared to that of ascorbic acid (for DPPH and nitric oxide determination) and gallic acid (for FRAP) as standards. The antioxidant mineral composition of the extract revealed the presence of zinc (1.82 ± 0.03 mg/100g) and selenium (0.59 ± 0.04 mg/100g). The antioxidant vitamins composition of the extract showed moderate concentrations of vitamin C (0.35 ± 0.01 mg/100 g) and vitamin E (0.68 ± 0.07 mg/100g). The result of this study revealed that methanol extract of Duranta erecta leaves could be a good source of antioxidants.

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