scholarly journals Serum exosomal miR-122-5p, GAS, and PGR in the non-invasive diagnosis of CAG

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 1350-1355
Author(s):  
Naihua Liu ◽  
Yuancheng Huang ◽  
Fengbin Liu ◽  
Hong Liu
Keyword(s):  
Atrophic Gastritis ◽  
Operating Characteristic ◽  
Roc Curves ◽  
Chronic Atrophic Gastritis ◽  
Non Invasive ◽  
Significant Difference ◽  
Clinicopathologic Factors ◽  
Polymerase Chain ◽  
Serological Biomarkers ◽  
Exosomal Mirna

Abstract Objective The aim of this study was to integrate the serum exosomal miRNA miR-122-5p with canonical serological biomarkers for the non-invasive screening of chronic atrophic gastritis (CAG) patients. Methods miR-122-5p and U6 were amplified by the quantitative reverse transcription polymerase chain reaction (RT-qPCR), gastrin (GAS), pepsinogen I (PG-I), and PG-II and were measured by ELISA. The area under the receiver operating characteristic (ROC) curves and their correlation were analyzed. Results In the present study, GAS level and PG-I/PG-II ratio (PGR) were increased in CAG group, but there was no significant difference in PG-I or PG-II levels between CAG group and chronic non-atrophic gastritis (CNAG) group. Only GAS level and PG-I/PG-II ratio were significantly correlated with atrophy, and not any other clinicopathologic factors. Expression of hsa-miR-122-5p positively correlated with GAS level, PG-I level, and PGR, while it negatively correlated with PG-II level; however, none of them had significant difference. The combination of GAS, PGR, and hsa-miR-122-5p presented as a better model for non-invasive screening of CAG compared to others. Conclusion These results suggested that serum exosomal hsa-miR-122-5p combined with GAS and PGR would elevate accuracy and specificity in non-invasive screening of CAG.

Three plasma-based microRNAs as potent diagnostic biomarkers for endometrial cancer

2021 ◽  
pp. 1-12
Author(s):  
Xingchen Fan ◽  
Minmin Cao ◽  
Cheng Liu ◽  
Cheng Zhang ◽  
Chunyu Li ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
External Validation ◽  
Diagnostic Value ◽  
Diagnostic Biomarkers ◽  
Non Invasive ◽  
Polymerase Chain ◽  
Public Datasets ◽  
Plasma Mirnas

BACKGROUND: MicroRNAs (miRNAs), with noticeable stability and unique expression pattern in plasma of patients with various diseases, are powerful non-invasive biomarkers for cancer detection including endometrial cancer (EC). OBJECTIVE: The objective of this study was to identify promising miRNA biomarkers in plasma to assist the clinical screening of EC. METHODS: A total of 93 EC and 79 normal control (NC) plasma samples were analyzed using Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) in this four-stage experiment. The receiver operating characteristic curve (ROC) analysis was conducted to evaluate the diagnostic value. Additionally, the expression features of the identified miRNAs were further explored in tissues and plasma exosomes samples. RESULTS: The expression of miR-142-3p, miR-146a-5p, and miR-151a-5p was significantly overexpressed in the plasma of EC patients compared with NCs. Areas under the ROC curve of the 3-miRNA signature were 0.729, 0.751, and 0.789 for the training, testing, and external validation phases, respectively. The diagnostic performance of the identified signature proved to be stable in the three public datasets and superior to the other miRNA biomarkers in EC diagnosis. Moreover, the expression of miR-151a-5p was significantly elevated in EC plasma exosomes. CONCLUSIONS: A signature consisting of 3 plasma miRNAs was identified and showed potential for the non-invasive diagnosis of EC.

scholarly journals Serum microRNAs are non-invasive biomarkers for the presence and progression of subarachnoid haemorrhage

2017 ◽  
Vol 37 (1) ◽  
Author(s):  
Nian-sheng Lai ◽  
Jia-qi Zhang ◽  
Fei-yun Qin ◽  
Bin Sheng ◽  
Xing-gen Fang ◽  
...  
Keyword(s):  
Subarachnoid Haemorrhage ◽  
Quantitative Pcr ◽  
Operating Characteristic ◽  
Roc Curves ◽  
Pcr Analysis ◽  
Healthy Controls ◽  
Modified Rankin Scale ◽  
Serum Samples ◽  
Non Invasive ◽  
Serum Micrornas

miRNAs are important regulators of translation and have been associated with the pathogenesis of a number of cardiovascular diseases including stroke and may be possible prognostic biomarkers. The purpose of the present study was to determine the expression levels of miRNAs in the sera of subarachnoid haemorrhage (SAH) patients and to evaluate their relationships with the severity and clinical outcome of SAH. Serum samples on day 3 after the onset of SAH were subjected to microarray analysis with Exqion miRCURYTM LNA array and quantitative PCR analysis. Serum samples from SAH patients (n=60) and healthy controls (n=10) were subjected to quantitative PCR analysis. The severities and clinical outcomes of the SAH patients were evaluated with the WFNS grade and the Modified Rankin Scale (mRS). Three miRNAs, miR-502-5p, miR-1297 and miR-4320 were significantly up-regulated in the sera of SAH patients when compared with the healthy controls. The serum miR-502-5p and miR-1297 levels were significantly higher in the patients with severe SAH and a poor outcome than in those with mild SAH and a good outcome (P<0.05). The areas under the receiver operating characteristic (ROC) curves (AUCs) of miR-502-5p, miR-1297 and miR-4320 to distinguish the SAH patients from the healthy controls were 0.958 (P<0.001), 0.950 (P<0.001) and 0.843 (P<0.001) respectively. Taken together, these results indicate that miR-502-5p and miR-1297 are potentially valuable indicators of the diagnosis, severity and prognosis of SAH, and miR-4320 was a potentially valuable indicator of the diagnosis of SAH.

Digital Luminescence Radiography Using a Chest Phantom

1993 ◽  
Vol 34 (5) ◽  
pp. 440-444 ◽  
Author(s):  
K. Lyttkens ◽  
M. Kehler ◽  
B. Andersson ◽  
S. Carlsen ◽  
A. Ebbesen ◽  
...  
Keyword(s):  
Image Quality ◽  
Operating Characteristic ◽  
Roc Curves ◽  
Diagnostic Image Quality ◽  
Diagnostic Image ◽  
Digital Radiographs ◽  
Significant Difference ◽  
Digital Luminescence Radiography ◽  
Optical Discs

With the introduction of picture and archiving communicating systems an alternative image display for the wards might be a personal computer (PC). The intention with this study was to evaluate the diagnostic image quality of the monitor of a PC compared to that of a workstation. Eighty-five digital radiographs of a chest phantom with simulated tumors in the mediastinum and right lung were saved on optical discs. The examinations were reviewed by 4 radiologists on a monitor at a workstation and at a PC, and receiver operating characteristic (ROC) curves were constructed. No significant difference was found between performance of the PC and the workstation.

scholarly journals miR-124 Functions as A Marker in Diagnosing Colorectal Cancer

2020 ◽  
Author(s):  
Yang Yan ◽  
Xiaohui Du ◽  
Shaoyou Xia ◽  
Songyan Li ◽  
Da Teng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Roc Curve ◽  
Operating Characteristic ◽  
Chain Reaction ◽  
Serum Samples ◽  
Optimal Cutoff ◽  
Clinicopathologic Factors ◽  
Healthy Control ◽  
Optimal Cutoff Point ◽  
Polymerase Chain

Abstract Background: MicroRNA-124 (miR-124) regulates cell growth, angiogenesis, invasion, and apoptosis of many human tumors. Our objective was to evaluate the levels of miR-124 in serum samples of colorectal cancer (CRC) patients to explore whether miR-124 in serum can be used as a biomarker for the detection of CRC.Methods: Serum miR-124 level was measured in 85 patients with CRC and 60 healthy control subjects using reverse transcription quantitative real-time polymerase chain reaction (qRT-PCR). The correlations between miR-124 levels and clinicopathologic factors were analyzed. Diagnostic performance of serum miR-124 level was calculated by using the receiver operating characteristic (ROC) curve.Results: The expression level of miR-124 in serum samples was significantly lower in CRC patients than in healthy controls (P<0.001). A positive correlation between miR-124 level and tumor size (P=0.010), invasion depth (P=0.021), lymph node metastasis (P=0.015), and TNM stage (P=0.004) was observed. The serum miR-124 yielded an AUC (areas under the ROC curve) of 0.832 with a sensitivity of 80.0% and a specificity of 81.7%, and the optimal cutoff point of miR-124 was 1.61.Conclusions: Serum miR-124 may have a potential as a novel biomarker for the detection of CRC.

scholarly journals Chronic atrophic gastritis: an update on diagnosis

2020 ◽  
Author(s):  
Adriana Botezatu ◽  
Nicolae Bodrug
Keyword(s):  
Atrophic Gastritis ◽  
Intestinal Metaplasia ◽  
Chronic Atrophic Gastritis ◽  
Diagnostic Methods ◽  
Digestive Endoscopy ◽  
Gastric Lesions ◽  
Inclusion Criteria ◽  
Non Invasive ◽  
Precancerous Gastric Lesions ◽  
Upper Digestive Endoscopy

Background and aim. Atrophic gastritis is a precancerous gastric lesion, therefore its early detection is a priority in preventing gastric cancer. The aim of the present paper is to develop a narrative synthesis of the present knowledge on diagnostic methods of chronic atrophic gastritis. Methods. A literature search was carried out on main databases: PubMed, Hinari, SpringerLink and Scopus (Elsevier) for the period 2000-2020. The searched keywords were: chronic atrophic gastritis, intestinal metaplasia and dysplasia + diagnosis. Inclusion criteria were focused on the articles about the invasive and non-invasive diagnosis of chronic atrophic gastritis and of precancerous gastric lesions, intestinal metaplasia and dysplasia; exclusion criteria were articles published before 2000 and those that did not include the proposed theme. Results. The search returned 575 papers addressing the topic of precancerous lesions. From these, 60 articles were qualified representative for the materials published on the topic of this synthesis article, being those that met the inclusion criteria. The data emphasize the need to use upper digestive endoscopy with biopsies for the diagnosis of chronic atrophic gastritis. However serological diagnosis is available as alternative mainly recommended in follow up. Conclusions. There are two main methodological approaches for the evaluation of chronic atrophic gastritis as a precancerous gastric lesions: invasive examination, which requires histological analysis of biopsy samples taken during upper digestive endoscopy, being the "gold standard" for diagnosis, and non-invasive serological examination using markers of gastric function.

scholarly journals Tumor-Derived Exosomal Long Noncoding RNAs as Promising Diagnostic Biomarkers for Prostate Cancer

2018 ◽  
Vol 46 (2) ◽  
pp. 532-545 ◽  
Author(s):  
Yu-Hui Wang ◽  
Jia Ji ◽  
Bi-Cheng Wang ◽  
Hao Chen ◽  
Zhong-Hua Yang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Tumor Invasion ◽  
Operating Characteristic ◽  
Specific Antigen ◽  
Roc Curves ◽  
Diagnostic Value ◽  
Healthy Individuals ◽  
Diagnostic Biomarkers ◽  
Non Invasive ◽  
Non Coding Rnas

Background/Aims: Exosomal circulating long non-coding RNAs (lncRNAs) in blood are emerging as clinically useful and non-invasive biomarkers for tumor diagnosis. However, normal cells can also secrete exosomes, so it is a prerequisite to obtain tumor-derived exosomes for better understanding of their diagnostic impacts in cancer. In this study, a dual-antibody-functionalized immunoaffinity system was established to isolate exosomes and investigate their lncRNAs expression pattern and clinical significance in prostate cancer (PCa). Methods: A commercially available kit was used to isolate total exosomes, which were then purified by a dual-antibody-functionalized immunoaffinity system. RT-qPCR was performed to detect the expression of exosomal lncRNAs. Receiver operating characteristic (ROC) curves were plotted to assess the diagnostic value. Results: Expression levels of two lncRNAs in tumor-derived exosomes were significantly higher than those in total exosomes. The levels of SAP30L-AS1 were upregulated in benign prostatic hyperplasia (BPH), and SChLAP1 levels were significantly higher in PCa than in BPH and healthy individuals. The area under the ROC curve indicated that SAP30L-AS1 and SChLAP1 had adequate diagnostic value to distinguish PCa from controls. Two lncRNAs separately combined with prostate specific antigen (PSA) possessed a moderate ability for discrimination. SAP30L-AS1 expression level was related to PSA values and tumor invasion. SChLAP1 expression was significantly higher in patients with higher Gleason scores, and was also effective in differentiating between BPH and PCa when the concentration of PSA was in the gray zone. Conclusion: The isolation of tumor-derived exosomes by dual-antibody-functionalized immunoaffinity systems and detection of their lncRNAs in plasma may lead to the identification of suitable biomarkers, with potential diagnostic utility.

The utility of six serum tumor markers in early detection of lung cancer

2019 ◽  
Author(s):  
Qingwen Jiang ◽  
Xu Zheng ◽  
Yiting Li ◽  
Weina Huang ◽  
Xinjian Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Sensitivity And Specificity ◽  
Operating Characteristic ◽  
Early Stage ◽  
High Sensitivity ◽  
Roc Curves ◽  
Serum Levels ◽  
Benign Diseases ◽  
Significant Difference ◽  
Diagnosis Of Lung Cancer

Abstract Background: Lung cancer has become the leading cause of cancer-related death in China. However, most of patients were diagnosed at advanced stage. Thus, novel lung cancer diagnostic tests, which can be used to screen individuals in early stage, are required.Methods: A total of 208 patients involving 161 cases of lung cancer and 47 cases of benign diseases were enrolled. Serum concentration of GTM, CETM, PTM, CTM, ETM and HTM were analyzed by kits according to the manufacturer’s guidelines.Results: The results showed significant difference in serum concentrations of GTM, CETM, PTM, CTM, ETM, and HTM between patients with lung cancer and benign diseases. In addition, when compared with benign diseases, higher levels of those six markers were also observed in patient with SCC and SCLC, but not for ADC. Receiver operating characteristic (ROC) curves were further suggested a high sensitivity and specificity of six markers to identify lung cancer.Conclusion: The serum levels of GTM, CETM, PTM, CTM, ETM and HTM in lung cancer were significantly higher than those of benign diseases. Moreover, these six biomarkers showed a high sensitivity and specificity to identify a patient with malignant. These findings suggested that detection of those six biomarkers in serum might be helpful for differential diagnosis of lung cancer.

Frequency of gene hMLH1 promoter methylation in the stomach antral and body area tissue of chronic atrophic pangastritis patients

2013 ◽  
Vol 20 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Rita Kupčinskaitė-Noreikienė ◽  
Dainius Jančiauskas ◽  
Elona Juozaitytė
Keyword(s):  
Atrophic Gastritis ◽  
Cpg Island ◽  
Methylation Status ◽  
Chronic Atrophic Gastritis ◽  
The Body ◽  
Body Area ◽  
Exact Test ◽  
Fisher's Exact Test ◽  
Significant Difference ◽  
Fisher’S Exact Test

Aim. To compare hMLH1 methylation frequency in stomach antral and body area tissue in chronic atrophic pangastritis patients, and to evaluate possible correlation severity of chronic atrophic gastritis markers. Methods. The study population consisted of 24 participants (with histologically confirmed chronic atrophic pangastritis), who underwent upper endoscopy. Biopsy specimens were taken from the gastric antral and body area. The methylation status of the gene hMlH1 was investigated. Results. Methylation of the CpG island of gene hMLH1 was found in the antral stomach area group 9/24, compared with the body area 2/24. There was a significant difference in gene methylation frequencies in the observed stomach parts (Fisher’s exact test, p = 0.04). There was a significant association between gene hMLH1 methylation and the occurrence of severe atrophic gastritis, intestinal metaplasia and the presence of hyperplastic mucosal changes (Fisher’s exact test, p 

Phantom Gastric Mucosa for Evaluating Computed Radiography in Double-Contrast Upper Gastrointestinal Examinations

1996 ◽  
Vol 37 (1P1) ◽  
pp. 204-207
Author(s):  
M. Murakami ◽  
H. Watanabe ◽  
H. Nakata
Keyword(s):  
Gastric Mucosa ◽  
Operating Characteristic ◽  
Computed Radiography ◽  
Roc Curves ◽  
Double Contrast ◽  
Significant Difference ◽  
Phosphor Plate ◽  
Visual Ranking ◽  
Upper Gastrointestinal ◽  
Better Than

Purpose: To test the clinical usefulness of computed radiography (CR) with a storage phosphor plate in upper gastrointestinal radiographic examinations, a newly devised phantom gastric mucosa was used. Material and Methods: Simulated small elevated and depressed lesions were created on a phantom gastric mucosa made from a styrofoam “plate”. Twenty-four sets of each CR and screen-film radiographs (SR) were obtained using phototimed exposures. Receiver operating characteristic (ROC) study and visual ranking using these images were performed. Results: There was no significant difference between the ROC curves of CR and SR. By visual ranking, CR was equal to or better than SR in most cases. In no case was SR definitely superior to CR. Conclusion: CR can be safely applied in upper gastrointestinal roentgenologic examinations.

scholarly journals A Survey on Main Semen Parameters in Natural Pregnancy and Intrauterine Insemination: Are There Any Significant Differences?

2016 ◽  
Vol 12 (3) ◽  
pp. 617-623 ◽  
Author(s):  
Roshanak Aboutorabi ◽  
Soudabeh Zamani ◽  
Yasaman Zarrin ◽  
Fatemeh Sadat Mostafavi
Keyword(s):  
Operating Characteristic ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Intrauterine Insemination ◽  
Roc Curves ◽  
Semen Parameters ◽  
Male Partners ◽  
Normal Morphology ◽  
Significant Difference ◽  
Reproductive Techniques

Intrauterine insemination (IUI) is a treatment of choice compared with other invasive and expensive techniques of assisted reproduction. Sperm quality is used to predict its outcome and success. Establishing threshold levels for sperm parameters is useful to avoid spending time and money to do other assisted reproductive techniques. This study was carried out to compare various semen parameters in a group of men eligible to participate in an IUI program with those of fertile men whose wives were pregnant at the time of the study. Two hundred and thirty-four semen samples were evaluated from subfertile men whose partners were candidates for IUI and 234 semen samples were evaluated from fertile men whose partners were pregnant less than 12 weeks. To assess the sensitivity and specificity of the main semen parameters, receiver operating characteristic (ROC) curves were used. Normal sperm morphology is more sensitive and specific compared with its progressive motility and concentration. No significant differences in various semen parameters of fertile men and those of the male partners of IUI candidates were observed. ROC analysis identified that sperm normal morphology using strict criteria may be a good indicator of fertility status in men. No significant difference in various semen parameters between the male partners of IUI candidates and the fertile men was seen. However, utilizing ROC curves, sperm morphology using strict criteria could be a good predictor of fertility.

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