Evaluation of selected serum biochemical and haematological parameters in gilts exposed per os to 100 ppb of zearalenone

2015 ◽  
Vol 18 (4) ◽  
pp. 865-872 ◽  
Author(s):  
E. Jakimiuk ◽  
J. Radwińska ◽  
A. Pomianowski ◽  
M. Woźny ◽  
K. Obremski ◽  
...  

Abstract Zearalenone (ZEN) widely contaminates animal feed of plant origin. The recommended safe concentrations of ZEN in feeds for various animal species are set mainly based on the mycotoxin’s hormonal properties (NOEL). Our growing knowledge about biologically active concentrations of ZEN, molecular mechanisms and cells/tissues targeted by ZEN indicates that the harmful effects exerted by this mycotoxin on animals may be far greater than previously believed. This experiment was performed on pre-pubertal gilts divided into a control group (n=9) and an experimental group (ZEN, n=9). The control group received placebo, whereas the experimental group was administered ZEN at a dose of 0.1 mg/kg feed (equivalent to 5 μg/kg BW/day) for 42 days. On days 14, 28 and 42 blood samples were collected from the animals to determine the concentrations of selected zearalenols, serum biochemical and haematological parameters. Conjugated ZEN was found in the blood serum of the experimental gilts. Changes in the analysed biochemical parameters included a transient increase in albumin and cholesterol levels. A statistically significant increase in the concentrations of neutrophilic and acidophilic granulocytes was observed in the white blood cell system. The results indicate that long-term per os exposure of pre-pubertal gilts to low doses of ZEN (below NOEL) has a modulatory effect on liver function and white blood cells.

Author(s):  
N. A. Ruban ◽  
O. S. Orishchuk ◽  
S. V. Tsap ◽  
L. M. Darmohray

One of the important conditions for the successful conduct of geese farming industry is normalized physiologically reasonable feeding birds that carried out by the use of complete feed and feed additives, including biologically active substances. Since days old, I (control) group of geese received during the research period, complete feed is made in terms of private enterprise «Orbita», II, III, IV and V (experimental) group received a complete feed with the addition of its composition of different doses of lecithin sunflower instead of a similar amount of soybean meal. At the beginning of the experiment live weight of geese had no significant differences in terms of research groups, indicating that similar selected poultry and averaged 116 – 118 grams. Since the first week of growing live weight of geese in experimental groups fed sunflower lecithin slightly increased. If, at the beginning it was not quite expressed, starting from the second week of growing this advantage becomes more significant. So, the live weight of geese at the age of two weeks in the control group was 1287 g, while the live weight of geese II, III, IV and V of experimental groups was higher by 1.4%, 4.3% (P < 0.01), 6.2% (P < 0.001) and 4.9% (P < 0.001), respectively. These differences observed between the experimental groups during the growing period. Thus, at the end of the first period of growth, namely four weeks of age, live weight of geese fourth experimental group was 3350 g, 8.0% (P < 0.001) higher relative to the control group by 5.6% compared to II experimental group, 2.8% (P < 0.01) III and 2.2% (P < 0.01) V research group. It should be noted that at the end of the second period, growing at the age of nine weeks goslings in control group matched the experimental group II on 2,3%, III – 5.1% (P <0.01), IV – 10.3% (P < 0.001) and V – 7.5% (P < 0.05), respectively. To study the anatomical and morphological structure of the experimental birds at the end of the scientific and economic experiment in the 60 days age were held control of slaughter. Our results indicate that different amounts of sunflower lecithin in diets of young geese during their growing meat differently affect their slaughter capacity. Table 3 shows that young geese feeding in the composition of animal feed sunflower lecithin has contributed to their live weight in experimental groups respectively 1.8%, 5.3%, 9.4% and 7.5% compared to the control group counterparts. For goose gut carcass weight preference geese in experimental group was respectively – 3.2%, 6.9%, 12.3% and 8.6%. The biggest muscle mass was observed in the experimental group IV geese and amounted to 1 895 g, that on 14.2% more muscle mass geese control group. The mass of muscle II, III and V of the research groups on the same indicator was higher by 3.0%, 7.7% and 9.2% in comparison with I control group. The use of sunflower lecithin in diets of young geese caused the increase in weight of internal fat. Thus this indicator preference experimental groups was – 5.7%, 15.4%, 25.1% and 18.3% according to analogue control. These changes have led to improved of meat and bone index. Thus, birds III, IV and V research groups preference for this indicator was 5.1%, 11.9% and 6.8% according to analogue controls. The indicator in poultry II experimental group was on par with the control group. Thus, putting in the diets of young geese sunflower lecithin positively affected the rate of growth of young geese slaughter their performance and made it possible to reduce the cost of feed per unit of growth. However, the best results were obtained when control weighing and slaughter, which is part of the feed was injected 0.4% sunflower lecithin.


2016 ◽  
Vol 16 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Vincenzo Monteverde ◽  
Fulvio Congiu ◽  
Irena Vazzana ◽  
Salvatore Dara ◽  
Sergio Guttadauro ◽  
...  

Abstract The aim of this study was to investigate the effect of omega-3 supplementation on some haematological parameters like red blood cells (RBC), haemoglobin (Hgb), haematocrit (Hct), white blood cells (WBC), neutrophils (Neu) and lymphocytes (Lym) that may have a direct effect on the performance of athletic horses. Ten regularly trained Standardbred horses (6 geldings and 4 females, 4-5 years old, mean body weight 500 ± 25 kg) were used for the study. They were randomly divided into two groups. The experimental group received an omega-3 dietary supplementation every day for 30 days. The control group received no supplementation. Every 10 days, horses took part in a 1660 metre harness race and blood samples were collected from each horse: one in the morning before race (pre) and one after race (post). The application of one-way analysis of variance for repeated mesures (ANOVA) showed a statistically significant difference due to the race in the two different groups. The results obtained in the present study show a discordant effect of supplementation with omega-3 on RBC, HCT and Hgb, while omega-3 supplementation has been shown to have a better effect on WBC, Neu and Lym, but further studies should be performed to better evaluate the benefits of these on the performance of the athletic horse.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


2019 ◽  
Vol 88 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Olga Aniołek

This research aimed to evaluate the effect of thyroid hormone deficiency on the erythrocytic system in dogs. Dogs with clinical symptoms of hypothyreosis such as obesity, hyperpigmentation, and lethargy were selected. The dogs demonstrating breed predisposition to hypothyreosis were incorporated in the analysis: Dachshunds, Retrievers, and mixed-breed dogs. A detailed history was taken and clinical, hormonal, biochemical and haematological blood tests were performed. Peripheral blood samples were taken from 53 dogs. Finally, the dogs with the initial T4 (thyroxine) concentration < 1.3 µg/dl and animals demonstrating clinical improvement after a 2-month therapy with levothyroxine at a dose of 10 µg/kg administeredper ostwo times a day were qualified. The animals between 10 months to 13 years of age were divided into two groups: clinically healthy (control group, n = 35) and dogs presenting clinical symptoms of hypothyreosis (experimental group, n = 18). In this research, the broadly described normocytic normochromic non-regenerative anaemia was not diagnosed in dogs with hypothyreosis. However, a positive correlation between T4 and red blood cell indices such as the average mass of haemoglobin per red blood cell, concentration of haemoglobin in a given volume of packed red blood cells as well as a negative correlation with haematocrit value was discovered in the experimental group after the 2-month therapy with levothyroxine. These results point to the influence of thyroid hormones on erythropoiesis. This observation is partially consistent with other studies, which noted the casual link between the changes in red blood cell system and the function of thyroid in dogs and humans.


Author(s):  
K. I. Khidirov ◽  
◽  
G. J. Kutlieva ◽  
B. I. Turaeva ◽  
N. A. Elova ◽  
...  

Research has been carried out to study the influence of "ProBioKorm Uz", a biologically active feed additive, on the development and physiological characteristics of rabbits of ‘New Zealand’ breed. During the experiment, 2 experimental and a control groups were formed. Adding "ProBioKorm Uz" to the main feed at the rate of 1% and 2% had an increase in experimental group rabbits’ live weight. Average live weight of them was higher by 5,7 %-3.8% than in the control group. Feed units composed 1.59%, dry matter 0.56 kg (3.61%) and crude protein 0.11 kg (3.59%) compared with the control group. Rabbits of experimental groups had higher indicators of erythrocytes concentration by 0.34-1.00 1012/l (10.34-19.53%), leukocytes - by 0.370-0.46 109/l (5.66-8, 06%), as well as a high level of hemoglobin by 7.42-11.08 g/l (6.81-10.08%) and total protein - by 2.40-4.48 (3.64-6, 42%).


2016 ◽  
Vol 47 (6) ◽  
Author(s):  
D. S. Dheyab

This study was conducted to investigate the effect of zinc in dose 15mg/kg.bw daily  taken by the mouth and dexamethasone 4mgIkg.Bw by injection for 30days on some hematological biochemical tests and some histological changes of liver spleen in male rabbits. Thirty rabbits were used that divided into 3 randomized groups (each group contain 10 male rabbits ). Control group was taken normal food and water, Zinc group that gave zinc at dose of 15mg/kg.BW/daily/oral on 1, 2, 3, 4 weeks. Dexamethasone with zinc group : Employ dexamethasone 4mg/Kg.Bw . I.M dialy for 1 and 2 weeks for experiment and at  3, 4th weeks they gave zn 15mg/lKg.Bw day/orally. Blood samples were taken from the heart directly in 2 and 4weeks to examine packed cell volume (pcv), white blood cells (WBCs), Red blood cells (RBCs) with differential Leuckcyte count.separation blood collection to plasma and examine glucose mg/dl , cholesterol mg/dl. In histological tests, rabbits were killed and separate their organs tissue from the body to examine liver and spleen. The results revealed  a decrease in level of RBCs, pcv after treatment with zinc 15, mg/Kg.Bw orally (zinc group) and increase in WBCs with differential leuckocyte count specially neutrophil cell, while biochemical tests show increase in glucose and cholesterol levels after treatment with dexamethasone 4mglkgBw. I/M seen increase in counts of RBCs , PCV, WBCs and differential lenkocyte count and decrease in glucose with cholesterol parameters, histological changes show change in liver after treatment by dexamethasone 4mglKg.Bw ,spleen tissue seen necrosis and pigmentation with hemorrhage after take dexamethasone 4mglkg in (dexamethasone + zinc group). Results also showed that zinc enhanced the immune system in at normal dose for limited time  because of its effect on other mineral such as copper and causes anemia , while the dexamethasone is a drug used for antianflammatory but for a short time.                                                                                                                           


Author(s):  
S. A. Shpуnova ◽  
O. A. Yadrishchenskaya ◽  
T. V. Selina ◽  
E. A. Basova

In order to increase the meat productivity of broiler chickens, increase production volumes and reduce the prime-cost of poultry products, it is necessary not only to ensure balanced feeding and compliance with production technology but also to include in the poultry diet non-traditional additives, biologically active substances that contribute to the normalization of metabolic processes and improve the digestibility of feed nutrients. One of these dietary additives is Betulin extracted from birch bark, which has antioxidant and immunomodulatory properties. In this regard, the purpose of the research was to study the influence of compound feed with the introduction of 2 % feed additive based on a synthetic microspherical nanoporous carbon carrier containing biologically active Betulin in its composition on the productivity of broilers. The results of studies on broiler chickens using compound feed with 2 % feed additive have been presented in the article. It has been found that the feed consumption per 1 kg of live weight gain in poultry of the experimental group have been lower by 9,5 % due to better absorption of nutrients, the live weight of broiler chickens of the experimental group at the age of 42 days was 2625,6 g and it was higher than the control group by 3,68 %. The digestibility of crude protein and fat reached 92,73 and 88,03 %, which exceeded the control group by 1,03 and 3,03 abs.%, respectively. It has been found that the use of compound feed with 2 % of the feed additive with the biologically active substance is of practical importance in connection with the increase in the productivity of broilers and the level of profitability of meat production by 8,2 abs.%.


2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Anna Piotrowska ◽  
Magdalena Zając ◽  
Łukasz Tota ◽  
Olga Czerwińska ◽  
Natalia Totko-Borkusiewicz ◽  
...  

Introduction. Pollen is a plant-based product and is a rich source of biologically active compounds such as: proteins, amino acids, carbohydrates, fats and fatty acids, phenolic compounds and enzymes, considered as an promising ingredient in cosmetic formulations. Aim. The aim of this study was to analyze the effect of a cream enriched with micronized bee pollen on selected skin characteristics evaluated in subjective and objective manner. Material and methods. The participants were 13 female volunteers (students) who met the following criteria: no dermatological and endocrine diseases, non-smoking and no special or elimination diets up to 3 months before the study, and also a negative allergy test result. Students were randomly assigned to the following groups: the experimental group – subjects using cream with 3% micronized bee pollen and the control one using cream base. Before and after the 5-day treatment, the participants undewent the measurements of skin hydration. The subjective evaluation of selected skin properties was examined by using a 3-grade scale. Results. All skin characteristics evaluated subjectively improved as a result of pollen-enriched formula treatment. The participants of the experimental group have noticed a significant change in the following properties: moisturizing, smoothness and skin tightening. The final measurement of skin hydration has showed an increase of 12.14 units in the experimental group and 4.52 units in the control group, indicating difference between groups (p = 0.0389). Also a positive correlation between application time and moisturizing (R = 0.929) has been found. Conclusions. The micronized bee pollen, as an active ingredient in protective and moisturizing preparations, seems to be an effective ingredient.


Author(s):  
S. S. Mirgalimova ◽  
A. O. Trufanova ◽  
R. R. Fatkullin

The physiological state of animals is to a certain extent characterized by hematological indicators, since blood occupies a special place in the body. By transferring nutrients and biologically active substances, the blood performs the general regulation of vital functions of the body. The use of Vitartil as a feed additive in the diets of animals leads to an increase in glycolytic processes and a decrease in the body’s need for oxygen delivery, which contributes to the growth of the animal body. The purpose of the research was to study the changes in blood parameters taking into account the age of Hereford steers against the background of the use of Vitartil feed additive to the main diet and its effect on the productive traits of animals. In order to carry out the experiment two groups of steers-analogs of Hereford breed have been formed at the age of 6 months per 10 heads in each. The difference between the groups was that the steers of the experimental group have been received the feed additive Vitartil in addition to the main diet. The greater yield of the meat part was in the steers of the experimental group in comparison with the herdmates of the control group. This indicator in the experimental group was 78,9 %, while in the control group it was lower by 1,6 %. The animals of the experimental group were superior to their herdmates in terms of meat weight by 1,4 kg or by 8,8 %. The relative number of bones was lower in the experimental group and amounted to 18,4 %, and in the control group 19,2 %. The meat content index was high in the steer have been received the biologically active additive Vitartil and was 4,3, and in the control group 4,0. It has been found that the use of Vitartil feed additive to the main diet of Hereford steers increases the blood content of hemoglobin and glucose, which has a certain effect on the morphological and chemical composition of meat, as well as increases its energy value.


Author(s):  
B. S. Kaloev ◽  
M. O. Ibragimov ◽  
M. M. Shagaipov

The reliability of the results obtained in scientific and economic experiments on the study of the effectiveness of the use of various feed components, including biologically active substances in the rearing of broilers needs to be confi rmed in production conditions. The purpose of the research was to study the joint use of enzyme drugs and lecithin in the rearing of broiler chickens. The results of production experiment on the study of the effectiveness of the joint inclusion of enzyme drugs Sunzyme and Sunfyse 5000 as well as lecithin in the diet of broiler chickens of the cross Ross-308 have been presented in the article. Production tests have been carried out under the conditions of the breeding farm “Achkhoy– Martanovsky” in the Chechen Republic. The duration of the experiment was 45 days. For research on the principle of analog groups two groups have been formed: a control group and an experimental group of 1000 heads in each. The broilers of the control group have been fed compound feed prepared in accordance with the growing period and consisting mainly of grain components of local production. The broilers of the experimental group have been fed the same compound feed, but with the addition of two enzyme drugs Sanzyme and Sanfyse 5000 (100 g/t of feed) as well as lecithin (10 g/kg of feed). The results of the production experiment fully confi rmed the data obtained in the scientific and economic experiment. The positive effect of the joint use of the studied enzyme drugs and lecithin has been manifested in all the studied productive traits. In particular, the increase in live weight of broilers increased by 12,1 %, slaughter yield by 3,5 %, the yield of carcasses of the 1st category by 3,0 %. The main economic indicators also found their confirmation: feed consumption per unit of growth decreased by 11,7 %, additional profit was received in the amount of 27308,2 rubles, the level of profitability of broiler rearing increased by 18,4 %.


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