scholarly journals Enzymatic Hydrolysis of Lignocellulose for Bioethanol Production

2017 ◽  
Vol 71 (4) ◽  
pp. 275-279
Author(s):  
Linda Rozenfelde ◽  
Māris Puķe ◽  
Irēna Krūma ◽  
Ieva Poppele ◽  
Nataļja Matjuškova ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Effect Of Temperature ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Temperature ◽  
Different Temperatures ◽  
C 50 ◽  
Hydrolysis Of ◽  
Temperature Time ◽  
Furfural Production

Abstract The effect of temperature, time and amount of enzyme on hydrolysis of wheat straw lignocellulose remaining after furfural production was studied. The residual substrate was subjected to enzymatic hydrolysis at different temperatures — 45 °C, 50 °C and 55 °C. Hydrolysis time was 72 hours, and samples were taken every 24 hours. The maximum glucose yield (76.5% of the theoretically possible) was reached when hydrolysis temperature 50 °C was used. The production rate of glucose increased with a hydrolysis period of time. The yield of glucose significantly depended on the ratio of enzyme to substrate.

Hydrolysis of S. platensis Using Sulfuric Acid for Ethanol Production

2022 ◽  
Vol 1048 ◽  
pp. 451-458
Author(s):  
Megawati ◽  
Astrilia Damayanti ◽  
Radenrara Dewi Artanti Putri ◽  
Zuhriyan Ash Shiddieqy Bahlawan ◽  
Astika Arum Dwi Mastuti ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Ethanol Production ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Second Step ◽  
Carbohydrate Composition ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Temperature ◽  
Hydrolysis Of

S. platensis is a microalga that contains carbohydrate composition of 30.21% which makes it potential to be used as raw material for ethanol production. Hydrolysis of S. platensis is the first step for converting its carbohydrates into monosaccharides. The second step is fermentation of monosaccharides into ethanol. This research aims to study the effect of temperature and microalgae concentration on the hydrolysis of S. platensis using sulfuric acid as catalyst. This research was conducted using 300 mL sulfuric acid of 2 mol/L, hydrolysis temperatures of 70, 80 and 90 °C, and microalgae concentrations of 20, 26.7, and 33.3 g/L. The effect of temperature is significant in the hydrolysis of S. platensis using sulfuric acid. At microalgae concentration of 20 g/L and hydrolysis time of 35 minutes, the higher the temperatures (70, 80, and 90 °C), the more the glucose yields would be (8.9, 13.5, and 22.9%). This temperature effect got stronger when the hydrolysis was running for 15 minutes. Every time the hydrolysis temperature increased by 10 °C, the glucose yield increased by 13.0% at microalgae concentration of 33.3 g/L. At temperature of 90 °C and time of 35 minutes, the higher the microalgae concentrations (20, 26.7, and 33.3 g/L), the higher the glucose yields would be (25.5, 27.7, and 28.2%). The highest glucose concentration obtained was 2.82 g/L at microalgae concentration of 33.3 g/L, temperature of 90 °C, and time of 35 minutes.

scholarly journals Assessment of Natural Deep Eutectic Solvent Pretreatment on Sugar Production from Lignocellulosic Biomass

2018 ◽  
Vol 152 ◽  
pp. 01014 ◽  
Author(s):  
Yoon Li Wan ◽  
Yuen Jun Mun
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Economic Feasibility ◽  
Effect Of Temperature ◽  
Sago Waste ◽  
Natural Deep Eutectic Solvent ◽  
Hydrolysis Of ◽  
Sugar Loss

Before the conversion of lignocellulosic biomass into fuel such as ethanol, the biomass needs to be pretreated and the yield of ethanol is highly dependent on the pretreatment efficiency. This study investigate the performance of deep eutectic solvent (DES) in pretreating sago waste which is a type of starchy biomass. The suitable type of DES in sago waste pretreatment was selected based on three criteria, which is the structural characteristic, the sugar yield during enzymatic hydrolysis and the amount of sugar loss during pretreatment. In this study, three types of DES namely Choline Chloride-Urea (ChCl-Urea), Choline Chloride-Citric acid (ChCl-CA) and Choline Chloride-Glycerol (ChCl-Glycerol) was investigated. The effect of temperature and duration on DES pretreatment was also investigated. All DES reagents were able to disrupt the structure and increase the porosity of sago waste during pretreatment. ChCl-Urea was selected in this study as it shows apparent structural disruption as examined under Scanning Electron Microscope (SEM). The highest glucose yield of 5.2 mg/mL was derived from enzymatic hydrolysis of ChCl-Urea pretreated sago waste. Moreover, reducing sugar loss during ChCl-Urea pretreatment was low, with only 0.8 mg/mL recorded. The most suitable temperature and duration for ChCl-Urea pretreatment is at 110°C and 3 hr. In a nutshell, the application of DES in pretreatment is feasible and other aspects such as the biodegradability and recyclability of DES is worth investigating to improve the economic feasibility of this pretreatment technique.

scholarly journals OPTIMIZATION FOR BATCH PROTEOLYTIC HYDROLYSIS OF SPENT BREWER’S YEAST BY USING PROTEASES

2018 ◽  
Vol 54 (4A) ◽  
pp. 181
Author(s):  
Nguyen Thi Thanh Ngoc
Keyword(s):  
Ph Value ◽  
Degree Of Hydrolysis ◽  
Optimal Conditions ◽  
Brewer’S Yeast ◽  
Hydrolysis Time ◽  
Brewer's Yeast ◽  
Factors Influencing ◽  
Hydrolysis Temperature ◽  
Hydrolysis Of ◽  
Proteolytic Hydrolysis

The yield of proteolylic hydrolysis for spent brewer’s yeast by protease and aminoacid contents of hydrolysates (the main  factors influencing in taste of hydrolysed product) depends on factors influencing in catalytic activities of enzymes as temperature, pH value, type of used  enzymes and ratio enzyme/substrate. With the purpose to hydrolyse the spent brewer’s yeast for food application, bitterness of hydrolysate takes the firth consideration, and than the yield of hydrolysing process plays economic role. In this paper, it is dealt with determination of optimal conditions to obtain the highest yield of hydrolysis process and the lowest bitterness of hydrolysate (the bitterness is determined by sensory evaluation, expressed equivalently with concentration of quinine). Response surface methodology (RSM) was used to determine optimum condition for batch proteolytic hydrolysis of spent brewer’s yeast. The influencing factors were investigated as temperature (X1): 40 oC–60 oC; pH (X2): 6.0–9.0, ratio E (flavourzyme)/S (X3): 5–10 U/g and hydrolysis time (X4): 6–9 hours. The experimental responses including degree of hydrolysis (Y1) (%) and bitterness of hydrolysate (Y2) (μmol quinine/ml) are performed in second-degree model. The optimal conditions for obtaining high degree of hydrolysis and low bitterness are determined: Ratio of enzyme mixture (alcalase 7.5 U/g and flavourzyme 8.5 U/g), pH 7.5, hydrolysis temperature at 52oC and hydrolysis time 9 hours. Under the optimal conditions, the actual values obtained for the yield of hydrolysis was 40.81  ± 0.044 % and the bitterness equivalently with concentration of quinine was 16.37 ± 0.03 μmol quinine/ml.

scholarly journals Study of the effect of process parameters on the yield of fermentable sugar from red cocoyam (Xanthosoma sagittifolium) peels via acid and enzyme hydrolysis

2021 ◽  
Vol 1 (1) ◽  
pp. 061-069
Author(s):  
Onoh Ikechukwu Maxwell ◽  
Anho Lawrence Oghenerivwe ◽  
Egwuagu Onyekachi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Functional Groups ◽  
Process Parameters ◽  
Maximum Yield ◽  
Proximate Analysis ◽  
Enzyme Hydrolysis ◽  
Glucose Yield ◽  
Xanthosoma Sagittifolium ◽  
Hydrolysis Of ◽  
Fermentative Process

The aim of this work is to study the acid and enzymatic hydrolysis of cocoyam peels using HCl, H2S04 acids and cellulase enzyme. The cellulase was secreted from Aspergillus Niger (A. niger) fungi. The proximate analysis of the substrate showed that cocoyam peel is a lignocellulosic biomass with a cellulose composition of 48%. The effect of the process parameters (time, temperature, acid concentration and pH) on the yield of glucose in acid and enzymatic hydrolysis of the cocoyam peel was respectively investigated. Maximum glucose yield of 44.5% was obtained after 3 days of enzymatic hydrolysis at 30°C and pH 5. The HCl acid hydrolysis showed a maximum glucose yield of 27.3% at 70°C, 5% HCl after 180 minutes. The glucose yield in H2S04 hydrolysis was relatively lower than that of the HCl with a maximum yield of 26.5% at 70°C, 5% H2SO4 after 180 minutes. In addition to, the functional groups present in the glucose synthesized from cocoyam ground peels and the standard glucose were evaluated using Fourier Transformed Infrared (FTIR). The FTIR results showed similarities in the functional groups present in both sugars. Cocoyam peel can be used for the production of glucose and further fermentative process to produce ethanol.

scholarly journals Hydrolysate from Mussel Mytilus galloprovincialis Meat: Enzymatic Hydrolysis, Optimization and Bioactive Properties

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5228
Author(s):  
Sara A. Cunha ◽  
Rita de Castro ◽  
Ezequiel R. Coscueta ◽  
Manuela Pintado
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Mytilus Galloprovincialis ◽  
Target Market ◽  
Hydrolysis Time ◽  
Bioactive Properties ◽  
Hydrolysis Temperature ◽  
Market Criteria ◽  
Enzyme Ratio ◽  
Production Conditions

Mussel production generates losses and waste since their commercialisation must be aligned with target market criteria. Since mussels are rich in proteins, their meat can be explored as a source of bioactive hydrolysates. Thus, the main objective of this study was to establish the optimal production conditions through two Box–Behnken designs to produce, by enzymatic hydrolysis (using subtilisin and corolase), hydrolysates rich in proteins and with bioactive properties. The factorial design allowed for the evaluation of the effects of three factors (hydrolysis temperature, enzyme ratio, and hydrolysis time) on protein/peptides release as well as antioxidant and anti-hypertensive properties of the hydrolysates. The hydrolysates produced using the optimised conditions using the subtilisin protease showed 45.0 ± 0.38% of protein, antioxidant activity via ORAC method of 485.63 ± 60.65 µmol TE/g of hydrolysate, and an IC50 for the inhibition of ACE of 1.0 ± 0.56 mg of protein/mL. The hydrolysates produced using corolase showed 46.35 ± 1.12% of protein, antioxidant activity of 389.48 ± 0.21 µmol TE/g of hydrolysate, and an IC50 for the inhibition of ACE of 3.7 ± 0.33 mg of protein/mL. Mussel meat losses and waste can be used as a source of hydrolysates rich in peptides with relevant bioactive properties, and showing potential for use as ingredients in different industries, such as food and cosmetics, contributing to a circular economy and reducing world waste.

Effect of Temperature and Water Immersion on the Mechanical Properties of Coated Fabrics

2010 ◽  
Vol 129-131 ◽  
pp. 230-234
Author(s):  
Ying Ying Zhang ◽  
Qi Lin Zhang ◽  
Chuan Zhi Zhou ◽  
Ying Zhou
Keyword(s):  
Mechanical Properties ◽  
Water Immersion ◽  
Effect Of Temperature ◽  
C 23 ◽  
Different Temperatures ◽  
Temperature Induction ◽  
Effects Of Temperature ◽  
C 50 ◽  
Coated Fabrics ◽  
Increasing Temperature

As composite, the mechanical properties of coated fabrics are sensitive to environment. This paper presented mechanical properties under different environments. A list of uniaxial tests are carried out under different temperatures including -20°C, 0°C, 23°C, 50°C, and 70°C. First, the tensile behaviors at room temperature and the failure behaviors are studied. Then, the effects of temperature on mechanical properties are determined. Finally, the effects of water immersion on mechanical properties are discussed. Results show PTFE coated fabrics remained unchanged in varying temperature and humidity. The temperature has effects on the mechanical properties of PVC coated fabrics. With increasing temperature, the strength decrease and the strain at break increase. The temperature induction factors are proposed for the design and analysis. The water immersion has little effect on the mechanical properties because of the impervious coating.

scholarly journals Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4552 ◽  
Author(s):  
Merve Nazli Borand ◽  
Asli Isler Kaya ◽  
Filiz Karaosmanoglu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Liquid Fraction ◽  
Sugar Content ◽  
Release Time ◽  
Variable Parameters ◽  
Glucose Yield ◽  
Steam Explosion Pretreatment ◽  
Saccharification Yield ◽  
Hydrolysis Of

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Optimization of enzymatic protein hydrolysis conditions of Asiatic hard clam (Meretrix meretrix)

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 153-162
Author(s):  
M.K. Zainol ◽  
F.W. Abdul Sukor ◽  
A. Fisal ◽  
T.C. Tuan Zainazor ◽  
M.R. Abdul Wahab ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Protein Hydrolysate ◽  
Hard Clam ◽  
Degree Of Hydrolysis ◽  
Protein Hydrolysis ◽  
Meretrix Meretrix ◽  
Optimum Conditions ◽  
Hydrolysis Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

This study was aimed to optimise the Alcalase® enzymatic hydrolysis extraction of Asiatic hard clam (AHC) (Meretrix meretrix) protein hydrolysate in terms of hydrolysis time, hydrolysis temperature, hydrolysis pH, and concentration of enzyme. Protein hydrolysate produced from AHC (M. meretrix) meat was used to determine the optimum hydrolysis conditions. Hydrolysis of AHC meat was optimised using the Central Composite Design Response Surface Methodology (RSM) (CCD). The relationship between four parameters such as temperature (45 – 65°C), enzyme to substrate concentration (1 – 2%), hydrolysis time (60 – 180 mins), and pH (7.5 – 9.5) to the degree of hydrolysis was investigated. The optimum conditions for enzymatic hydrolysis of AHC meat to achieve the maximum degree of hydrolysis (DH) were observed at 65°C, enzyme to substrate concentration of 1%, hydrolysis time of 60 mins, and pH 7.5. The enzymatic protein hydrolysis of AHC meat was predicted using a two factors interaction (2FI) model. Under these optimum conditions, DH's predicted value was 97.41%, which was close to the experimental value (97.89%). The freeze-dried protein hydrolysate powder was characterized concerning the proximate composition. Proximate analysis revealed that the AHC meat contains 7.92±1.76% of moisture, 2.23±0.89% of crude fat, 1.98±0.82 of ash, and 10.53±0.04% of crude protein. While the Asiatic hard clam protein hydrolysate (AHCPH) composed 9.12±0.02% of moisture, 0.80±0.29% of crude fat, and 27.76±0.10% of ash. The protein hydrolysate produced also contained high protein content (50.09±0.88%) and may serve as a good protein source.

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