Screening of Antibacterial Activities of Twenty-One Oxygenated Monoterpenes

2007 ◽  
Vol 62 (7-8) ◽  
pp. 507-513 ◽  
Author(s):  
Recep Kotan ◽  
Saban Kordali ◽  
Ahmet Cakir
Keyword(s):  
Essential Oils ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Variable Degree ◽  
Bacterial Strains ◽  
Weak Zone ◽  
Plant Essential Oils ◽  
Oxygenated Monoterpenes ◽  
Limonene Oxide

Plant essential oils are widely used as fragrances and flavours in the cosmetic, perfume, drug and food industries. Oxygenated monoterpenes are widespread components of the essential oils, usually occurring in high amount. In this paper, the antibacterial activities of twenty-one oxygenated monoterpenes (borneol, borneol acetate, camphor, carvone, 1,8-cineole, citronellal, β-citronellol, dihydrocarvone, fenchol, fenchone, geraniol acetate, isomenthol, limonene oxide, linalool, linalool acetate, nerol, nerol acetate, terpinen-4-ol, α-terpineol, menthol and menthone) and penicillin (standard antibiotic) were determined using a disc diffusion method (in vitro) against 63 bacterial strains, belonging to 37 different genera and 54 species (plant, food and clinic origins). The results showed that the oxygenated monoterpenes exhibited a variable degree of antibacterial activities. These compounds also inhibited the growth of bacterial strains by producing a weak zone of inhibition from 7 to 11 mm in diameter, depending on the susceptibility of the tested bacteria. Among the tested compounds, nerol, linalool α-terpineol, fenchol and terpinen-4-ol showed antibacterial activity at a broad spectrum. However, their antibacterial activities were lower than those of penicillin. In contrast to these compounds, camphor and 1,8-cineole exhibited no inhibition effects on the growth of all tested bacteria

scholarly journals The influence of plant essential oils on in vitro growth of Pectobacterium and Dickeya spp. bacteria

2021 ◽  
Vol 20 (6) ◽  
pp. 19-29
Author(s):  
Małgorzata Schollenberger ◽  
Agnieszka Gadomska-Gajadhur ◽  
Ewa Mirzwa-Mróz ◽  
Damian Kret ◽  
Ewa Skutnik ◽  
...  
Keyword(s):  
Essential Oils ◽  
Soft Rot ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Juniperus Virginiana ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
First Report ◽  
Plant Essential Oils

 The activity of essential oils from Eucalyptus globulus, Pinus silvestris, Lavandula angustifolia, Juniperus virginiana, Rosmarinus officinalis and Citrus paradise against the soft-rot pathogens Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum, Pectobacterium parmentieri and Dickeya solani was determined in vitro. The antibacterial activity of the essential oils will be evaluated using the disk-diffusion method by Kirby-Bauer [Bauer et al. 1966]. It was found that all the presented essential oils varied in antimicrobial activity against the four bacterial strains. No differences in the influence of streptomycin on inhibition of growth of the four bacterial strains were observed. Among six tested plants, essential oils from P. sylvestris had the strongest inhibitory effect on the growth of soft rot bacteria from Pectobacterium genus. This paper constitute the first report on the activity of the essential oils obtained from J. virginiana against soft rot bacteria. They are also the first report on the activity of the essential oils obtained from E. globulus, P. silvestris, L. angustifolia and C. paradisi against P. atrosepticum, P. parmentieri and D. solani as well as on the activity of the R. officinalis essential oils against P. atrosepticum and P. parmentieri.

scholarly journals Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

2010 ◽  
Vol 75 (6) ◽  
pp. 739-747 ◽  
Author(s):  
Dinesh Bisht ◽  
Rajendra Padalia ◽  
Lalit Singh ◽  
Veena Pande ◽  
Priyanka Lal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antagonistic Activity ◽  
Significant Activity ◽  
Variable Degree ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

scholarly journals ANTIBACTERIAL ACTIVITY OF SOAP MADE FROM ESSENTIAL OILS AND COMMERCIAL SOAP SOLD IN THE LEBANESE MARKET

2021 ◽  
pp. e304
Author(s):  
Candy GERGES ◽  
Mohamad HADLA ◽  
Michele ELIALI ◽  
Martine RICHA ◽  
Nour Mammari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Effective Measure ◽  
E Coli ◽  
Different Types ◽  
Commercial Soap ◽  
Zones Of Inhibition

Background: Thousands of people are dying as a result of infections caused by bacteria. Among the main routes of germ transmission are the hands, making hand hygiene very important in preventing the spread of pathogens and bacterial infection. Hand washing with soap and water is considered to be a simple and effective measure. Old soap manufacturers have long had traditional uses in the Lebanese community. Methods: The purpose of this study is to compare the antibacterial activity of oil-based soaps with commercial soap sold in the Lebanese market. Different types of herbal soap and antiseptics have been used in this study. Four bacterial strains were used: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter Spp. The antibacterial activities of these soaps were determined by the diffusion method of disks in agar medium. Results and Discussion: Statistical analysis of zones of inhibition showed that S. epidermidis, P. aeruginosa and Enterobacter Spp. were sensitive only to traditional oil-based soaps, which are Sage, Rose Mary and Cedar. In addition, S. aureus showed sensitivity to soaps comprised of essential oils as well as antibacterial synthetic soap, Dettol and Lifebuoy. On the other hand, E. coli showed resistance to all soaps. Soaps comprised of natural essential oils have shown antibacterial activity superior to so-called “Antibacterial” soaps. Conclusion: Based on this study, we can say that the use of soaps with essential oils might be the best option due to their organic origin as well as their antibacterial proved activities.

scholarly journals Plant Essential Oils Based Nanoemulsion Formulations and Its Antibacterial Effect on Some Pathogens

2019 ◽  
Vol 9 (1) ◽  
pp. 4800-4808
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Droplet Size ◽  
Bacterial Growth ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Natural Antimicrobials ◽  
Cinnamon Oil ◽  
Plant Essential Oils ◽  
Agar Well Diffusion Method

Antibiotic resistance is the most challenging problem of concern globally and this is invigorating the need of newer antimicrobial products with potential antimicrobial properties. Plant products, especially plant essential oils produce a large array of secondary metabolites as natural antimicrobials. Use of nanotechnology can add advantages to enhance the antibacterial properties of these essential oils. Present study is focused on development of nanoemulsions from plant essential oils and to study their antibacterial activities. Tea Tree Oil, Thyme Oil, Clove leaf and Cinnamon Essential Oils nanoemulsion was formulated using Tween 20 and Tween 80 respectively using probe ultrasonicator. All the formulated Nanoemulsions were then subjected to physicochemical characterization, stability studies and tested for antibacterial activities using Agar-well diffusion method. Stable nanoemulsion formulation with maximum antibacterial activity then subjected to droplet size measurements and polydispersibility index study. Increase in surfactant concentration resulted in reduction in droplet size when ultrasonication time was constant. Cinnamon oil nanoemulsion 20C4 & 80C4 with pdi index 0.573 and 0.382 and droplet size 272.3nm and 133.6 nm respectively demonstrated maximum antibacterial activity in Agar-well diffusion method against S.aureus, E.coli, and S.typhi. When both nanoemulsions were exposed to bacterial growth curve inhibition study. No potential rise in optical density of test pathogens were observed. The inhibition of bacterial growth may be due to killing action of cinnamon oil nanoemulsion formulations to initial bacterial inoculum added to nutrient broth. The study suggests that nanoemulsion formulations from plant essential oils can be used as natural antimicrobials in variety of products.

scholarly journals Chemical composition and antibacterial activities of Capparis spinosa essential oils from Algeria

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Halima Benachour ◽  
Messaoud Ramdani ◽  
Takia Lograda ◽  
Pierre Chalard ◽  
Gilles Figueredo
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Hexadecanoic Acid ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Capparis Spinosa

Abstract. Benachour H, Ramdani M, Lograda T, Chalard P, Figueredo J. 2020. Chemical composition and antibacterial activities of Capparis spinosa essential oils from Algeria. Biodiversitas 21: 161-169. The essential oils of Capparis spinosa L. collected from six locations in Algeria were obtained by hydro-distillation. The chemical composition of oils was performed by GC-MS. The disc diffusion method is applied for the antibacterial activity. The extraction produced low yield (0.03%). The result of chromatographic analysis (GC/MS) leads to the identification of 33 components; palmitic acid (38.19%), nonanal-n (12.61%), cymene-2,5-dimethoxy-para (8.94%) and octacosane (5.49%) were the major components of these oils. The result of cluster analysis based on essential oils constituents showed the presence of three chemotypes,i.e., the chemotype of Nonanal-n-Cymen 2,5 dimethoxy para-Dodecanal, the chemotype of Nonanal-n-Hexadecanoic acid-tetracosane and the chemotype Tetracosane-n-pentyl furane-2-octacosane. In-vitro antimicrobial activity of caper oils against nine bacterial species showed that the oils have no activity against E. coli and have modest activities against eight other bacterial species tested; however, the desirability test shows that the oils used were not effective on the bacteria tested.

Comparative Study on the Antibacterial Properties of Nanoemulsion of Zataria multiflora Essential Oil Fortified with Cinnamaldehyde Against Common Foodborne Pathogens

2020 ◽  
Vol 10 (5) ◽  
pp. 631-641
Author(s):  
Shahnaz Soufi ◽  
Majid Aminzare ◽  
Hassan Hassanzad Azar ◽  
Koorosh Kamali
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Suitable Alternative ◽  
Zataria Multiflora

Background: Nowadays, the application of several and natural preservatives in small quantity is a more preferred approach. In this regard, one of the effective methods is the formation of nanoemulsion of essential oils. Objective: The objective of this study was to compare the in vitro antibacterial activities of cinnamaldehyde (CIN) and Zataria multiflora essential oil in conventional (ZEO), nanoemulsion (NZEO) and fortified nanoemulsion (NZEOC) forms against common foodborne pathogens. Methods: Firstly, the Zataria multiflora essential oil was analyzed by GC-MS. The nanoemulsion of Z. multiflora essential oil was then prepared alone and fortified with cinnamaldehyde. Finally, their antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli was evaluated. Results: Based on the results, carvacrol (36.62%) was found to be the most important compound of essential oil. In disc diffusion and micro-dilution methods, the addition of CIN to ZEO during nanoemulsion formation (NZEOC) showed more antibacterial activity when compared to the individual addition of NZEO and CIN (NZEO+CIN). However, according to the vapor phase diffusion method, nano-treatments exhibited less inhibitory effects than the other treatments. Conclusion: It can be concluded that the fortification of essential oils with their derived pure compounds during nanoemulsion formation, can be used as a suitable alternative to chemical antibacterial compounds in the food industry.

scholarly journals Chemotherapeutic Potential of Carthamus Oxycantha Root Extract as Antidiarrheal and In Vitro Antibacterial Activities

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 226 ◽  
Author(s):  
Muhammad Ikram ◽  
Amany Magdy Beshbishy ◽  
Muhammad Kifayatullah ◽  
Adedayo Olukanni ◽  
Muhammad Zahoor ◽  
...  
Keyword(s):  
Magnesium Sulfate ◽  
Castor Oil ◽  
Research Work ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Root Extract ◽  
Bacterial Strains

Our research work was designed to investigate the curative and preventive effects of Carthamus oxycantha root extract against diarrhea and microorganisms. For the antibacterial experiment, the agar well diffusion method was used against standard bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, and Salmonella typhi, while for the assessment of antidiarrheal activity, castor oil and the magnesium sulfate-induced diarrhea method was used on albino, laboratory-bred (BALB/c) mice at a dose rate of 200 and 400 mg/kg (body weight, b.w) orally. The methanol extract of C. oxycantha significantly (p < 0.001) decreased the frequency of defecation, and wet stools in a dose depended on the manner of after receiving magnesium sulfate (2 g/kg (b.w)) and castor oil (1.0 mL/mice). Furthermore, the extract of C. oxycantha showed concentration-dependent antimicrobial properties against S. aureus followed by S. typhi, E. coli, and P. aeroginosa bacterial strains, with inhibitions ranging from 10.5–15 mm. These findings show significant results that C. oxycantha is effective as an antidiarrheal and antibacterial agent. However, further works are needed to establish its mode of action.

scholarly journals Chemical Characterisation and Biological Effects of Essential Oils of Four Gabonese Medicinal Plants

2021 ◽  
pp. 52-60
Author(s):  
Elvis Jolinom Mbot ◽  
Cédric Sima Obiang ◽  
Maximilienne Ascenssion Nyegue ◽  
Bill Raphaël Bikanga ◽  
Huguette Agnaniet ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oils ◽  
Biological Effects ◽  
Antibacterial Activities ◽  
Chemical Compositions ◽  
Aromatic Plants ◽  
Bacterial Strains

Aims: The objective of this work is to determine the chemical composition and in vitro evaluation of the antibacterial activities of essential oils of four species of aromatic plants of Gabonese origin obtained by hydrodistillation. Methods: All the samples were examined by chromatographic analyzes and by GC / MS coupling. Antimicrobial activity was assessed by diffusion and microdilution methods. Results: The most common compounds found in essential oils were terpene hydrocarbons and oxygenates. The major constituents are β-phellandrene (56.3%), β-pinene (11%) and myrcene (10.4%). The three bacterial strains used are sensitive to essential oils. However, some essential oils stood out with greater spectra of action compared to others, such as Maranthes gabunensis and Mammea africana which showed activity against all strains. The inhibition parameters (MIC and CMB) are between 0.78 and 25 mg / mL. Some oils have shown bacteriostatic and / or bactericidal activity on the targeted strains. Conclusion: The essential oils studied present a diversity of chemical compositions and good antibacterial activities.

scholarly journals Bactericidal And Antioxidant Effects of Domestic Essential Oils On Certain Pathogenic Bacteria.

2021 ◽  
Author(s):  
Faisal MB. Al-Sarraj ◽  
Ahmad M. Qumri
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Rapd Pcr

Abstract Background: Essential oils (EOs) are volatile, liquid substances that come from natural sources such as plants. As secondary metabolites of most plant species, that are typically found in leaf chloroplasts or from the hydrolysis of certain glycosides, their primary function is to combat attacks from predators as well as attract pollinators. Due to their biological activity, EOs have gained popularity in industries such as food processing, cosmetics, and personal care products as well as the pharmaceutical industry. In this study, the antimicrobial nature and antioxidant activity of Saudi Arabian EOs were compared with the antimicrobial activity of known antibiotics.Methods: The In vitro evaluation of antibacterial activity and antioxidant capacity of EOs collected from Jeddah domestic market for gram-negative and gram-positive pathogenic bacteria was carried out using the disc diffusion method. Disc diffusion method was used for the assessment of bactericidal activity of local EOs. The antioxidant activity was determined using phosphomolybdenum method. Furthermore, RAPD-PCR was used to compare the effects of these EOs on DNA in both untreated and treated pathogenic bacterial strains.Results and Conclusion: Bacterial strains with varying antibiotic resistance potential have been successfully isolated. The inhibition zone measurement revealed that the EOs used had some antibacterial effects on the bacterial strains as well as some selectivity on the bacterial strains that they acted on.

scholarly journals In VitroAntibacterial Activity of Essential Oils againstStreptococcus pyogenes

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Julien Sfeir ◽  
Corinne Lefrançois ◽  
Dominique Baudoux ◽  
Séverine Derbré ◽  
Patricia Licznar
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Origanum Compactum ◽  
Broth Dilution

Streptococcus pyogenesplays an important role in the pathogenesis of tonsillitis. The present study was conducted to evaluate thein vitroantibacterial activities of 18 essential oils chemotypes from aromatic medicinal plants againstS. pyogenes. Antibacterial activity of essential oils was investigated using disc diffusion method. Minimum Inhibitory Concentration of essential oils showing an important antibacterial activity was measured using broth dilution method. Out of 18 essential oils tested, 14 showed antibacterial activity againstS. pyogenes. Among themCinnamomum verum, Cymbopogon citratus, Thymus vulgaris CT thymol, Origanum compactum, andSatureja montanaessential oils exhibited significant antibacterial activity. Thein vitroresults reported here suggest that, for patients suffering from bacterial throat infections, if aromatherapy is used, these essential oils, considered as potential antimicrobial agents, should be preferred.

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