scholarly journals Chemical composition and antibacterial activities of Capparis spinosa essential oils from Algeria

Author(s):  
Halima Benachour ◽  
Messaoud Ramdani ◽  
Takia Lograda ◽  
Pierre Chalard ◽  
Gilles Figueredo

Abstract. Benachour H, Ramdani M, Lograda T, Chalard P, Figueredo J. 2020. Chemical composition and antibacterial activities of Capparis spinosa essential oils from Algeria. Biodiversitas 21: 161-169. The essential oils of Capparis spinosa L. collected from six locations in Algeria were obtained by hydro-distillation. The chemical composition of oils was performed by GC-MS. The disc diffusion method is applied for the antibacterial activity. The extraction produced low yield (0.03%). The result of chromatographic analysis (GC/MS) leads to the identification of 33 components; palmitic acid (38.19%), nonanal-n (12.61%), cymene-2,5-dimethoxy-para (8.94%) and octacosane (5.49%) were the major components of these oils. The result of cluster analysis based on essential oils constituents showed the presence of three chemotypes,i.e., the chemotype of Nonanal-n-Cymen 2,5 dimethoxy para-Dodecanal, the chemotype of Nonanal-n-Hexadecanoic acid-tetracosane and the chemotype Tetracosane-n-pentyl furane-2-octacosane. In-vitro antimicrobial activity of caper oils against nine bacterial species showed that the oils have no activity against E. coli and have modest activities against eight other bacterial species tested; however, the desirability test shows that the oils used were not effective on the bacteria tested.

2008 ◽  
Vol 73 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Nenad Vukovic ◽  
Tanja Milosevic ◽  
Slobodan Sukdolak ◽  
Slavica Solujic

This study was designed to examine the chemical composition of the essential oil and the in vitro antibacterial activities of the essential oil and methanol extract of Teucrium montanum. The inhibitory effects of the essential oil and the methanol extracts of Teucrium montanum were tested against thirteen bacterial species using the disc-diffusion method. GC/MS analyses revealed that the essential oil contained mainly sesquiterpenes, such as ?-cadinene (17.19 %), ?-selinene (8.16 %) and ?-calacorene (4.97 %). The highest activities were obtained with the essential oil of Teucrium montanum against K. pneumoniae, B. subtilis, B. mycoides, E. cloaceae and A. chlorococcum. In addition, comparison of the antibacterial activities of the essential oil and the methanol extract showed that the essential oil exhibited the stronger antibacterial activities.


2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Abdelkader Ounoughi ◽  
Messaoud Ramdani ◽  
Takia Lograda ◽  
Pierre Chalard ◽  
Gilles Figueredo

Abstract. Ounoughi A, Ramdani M, Lograda  T, Chalard P, Figueredo G. 2020. Chemotypes and antibacterial activities of Inula viscosa essential oils from Algeria. Biodiversitas 21: 1504-1517. The aim of this work is to investigate the chemical composition and the antibacterial activities of the essential oils from Inula viscosa (L.) Aiton. Samples were collected in the flowering stage, from different localities in Algeria. The aerial parts of I. viscosa were submitted to a hydro distillation. The chemical composition of the essential oil was analyzed by GC and GC/MS. The antibacterial activity of the essential oils was evaluated using the disc diffusion method against fifteen bacterial species. Fifty-eight compounds representing 98.93 ± 2.03% of the total oil was identified in I. viscosa. It was found that the chemical composition was dominated by the presence of the following major products: polygodial (19.8 ± 16.97%), phytol (12.3 ± 9.77%), fokienol (6.01 ± 3.43%), intermedeol neo (5.09 ± 2.38%), caryophyllene oxide (4.91 ± 3.03%), nerolidol-Z (4.46 ± 5.46%), nerolidol-E (4.24 ± 8.07%) and α-ionone iso methyl-E (3.72 ± 2.26%). The essential oil of I. viscosa has moderate activity against the bacteria tested. In contrast, the Escherichia coli ATCC 25922, Pseudomonon syringae ATCC 53543 and Enterococcus faecalis ATCC 49452 strains are resistant to I. viscosa essential oils. The phytochemical study of I. viscosa showed that it is rich in terpene compounds, with polygodial and phytol as major components. Three distinct chemotypes are highlighted. The (Polygodial-Intermedeol-neo-Phytol) chemotype of Salah Bey population and two chemotypes with Fokinol-polygodial and Fokinol-phytol. Moderate antibacterial activities of essential oils against the bacteria tested were found.


2021 ◽  
Vol 22 (2) ◽  
Author(s):  
YACINE MOHAMADI ◽  
TAKIA LOGRADA ◽  
Messaoud Ramdani ◽  
GILLES FIGUEREDO ◽  
PIERRE CHALARD

Abstract. Mohamadi Y, Lograda T, Ramdani M, Figueredo G, Chalard P. 2021. Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria. Biodiversitas 22: 933-946.  The objectives of this study were to determine the chemical composition and the antibacterial activities of Myrtus communis essential oils. The aerial parts of the myrtle were collected from nineteen localities of the Algerian coast, and subjected to hydrodistillation. The chemical composition of essential oils was analyzed using gas chromatography coupled with mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disk diffusion method against ten bacterial species. Essential oils of M. communis was pale yellow oils, very fragrant, with an average yield of 0.68 ± 0.6%. Forty-six compounds were identified, representing an average of 99.17 ± 0.6% of total oils. The α-pinene was the most abundant component in the essential oils of all populations studied, with a mean of 50.1 ± 10.3%, followed by 1,8-cineole (22.27 ± 4.32%), limonene (5.16 ± 2.67%), linalool (3.79 ± 2.97%), α-terpineol (2.75 ± 1.12%), geranyl acetate (1.95 ± 0.7%) and methyl eugenol (1.40 ± 0.98%). Myrtenyl acetate was only observed in four populations with significant levels (from 10.66 to 20.94%). Six chemotypes have been identified in these oils. The α-pinene – 1,8-cineole - limonene chemotype was found in the majority of populations. The chemotype of α-pinene - 1,8-cineole - α-terphenyl acetate characterizes the Oued El-Aneb population. The α-pinene - neryl formate - limonene chemotype is observed in the Honaïn population. The remaining populations are characterized by myrtenyl acetate. The essential oils of M. communis were effective against Staphylococcus aureus ATCC 25923, Salmonella enterica ATCC35659, Proteus mirabilis, and Escherichia coli ATCC 25922; however, no significant effect was observed on Klebsiella pneumonia ATCC 700603 and Serratia liquefaciens.


Author(s):  
Fatima El Kamari ◽  
Amal Taroq ◽  
Yassine El Atki ◽  
Imane Aouam ◽  
Badiaa Lyoussi ◽  
...  

Objective: The aim of the current study is to determine the chemical composition and evaluate antibacterial activity of Vitex agnus-castus L. (VAC) essential oils against some bacteria causing nosocomial infections in the neonatal and intensive care rooms at the university hospital center of Fez Morocco. Methods: The phytochemical screening of essential oils was determined using gas chromatography (GC) and GC-mass spectrometry analysis. The antibacterial test was evaluated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria species (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) using disc diffusion method. Results: Twenty-nine components were identified in the fruits’ oil representing 93.1% of total oil. The major components in the fruits oil are 1,8-cineole (11.6%), α-thujene (9.3%), phyllocladene (8.2%), α-pinene (7.9%), caryophyllene (5.9%), and cubenol (5%). Furthermore, 28 components were identified in the leaf essential oil. The main component was caryophyllene (9.5%), followed by 1,8-cineole (8.7%), manoyl oxide (7.3%), eugenyl acetate (7.1%), phyllocladene (6.8%), and α-pinene (5.2%). Antibacterial activity of both oils showed a strong activity against nosocomial bacteria tested. Conclusion: Essential oils of Moroccan VAC could be exploited as natural drugs for bacteria, especially those who have acquired resistance to conventional antibiotics.


2018 ◽  
Vol 34 (5) ◽  
pp. 2495-2501 ◽  
Author(s):  
Sarmd D. Noori ◽  
Mazin N. Mousa ◽  
Shaker A. N. Al-Jadaan

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.


2005 ◽  
Vol 60 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Ali Sonboli ◽  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Mohammad Reza Kanani

The chemical composition of the essential oils obtained from two samples (GP1 and GP2) of Grammosciadium platycarpum Boiss. was analyzed by GC and GC-MS. The analysis of the oils resulted in the identification of twenty-two constituents. Linalool (79.0% - GP1, 81.8% - GP2) and limonene (10.0%, 5.8%) were found to be the major components, respectively. The in vitro antibacterial activities of these oils and their main compounds against seven Gram-positive and Gram-negative bacteria were investigated. The results exhibited that the total oils and their major components possess strong to moderate activities against all the tested bacteria except for Pseudomonas aeruginosa.


2016 ◽  
Vol 8 (2) ◽  
pp. 209-216
Author(s):  
A. Rahim ◽  
R. Ali ◽  
A. Islam

 2',4',5'- and 2',3',4'-trimethoxy flavanones have been synthesized starting with 2-hydroxyacetophone and substituted aldehyde. Antibacterial activities of the flavanones have been tested along with their corresponding chalcones against two human pathogenic bacteria (Streptococcus-b-haemolyticus and  Klebsiella sp. (G-)). Antifungal activities of the flavanones have also been investigated against  two plants pathogenic mold fungi (Rhizactonia solani Sclerotium rolfsii). The structures of the synthesized compounds have been characterized with the help of  UV, IR and 1H NMR and 13C-NMR spectra. The antibacterial and antifungal screening were performed in vitro by the filter paper disc diffusion method and poisoned food technique. The flavanones showed antibacterial activity while no activity was observed to their corresponding chalcones against the tested bacteria. On the other hand, chalcones and their corresponding flavanones both showed fungicidal activities.


2021 ◽  
pp. e304
Author(s):  
Candy GERGES ◽  
Mohamad HADLA ◽  
Michele ELIALI ◽  
Martine RICHA ◽  
Nour Mammari ◽  
...  

Background: Thousands of people are dying as a result of infections caused by bacteria. Among the main routes of germ transmission are the hands, making hand hygiene very important in preventing the spread of pathogens and bacterial infection. Hand washing with soap and water is considered to be a simple and effective measure. Old soap manufacturers have long had traditional uses in the Lebanese community. Methods: The purpose of this study is to compare the antibacterial activity of oil-based soaps with commercial soap sold in the Lebanese market. Different types of herbal soap and antiseptics have been used in this study. Four bacterial strains were used: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter Spp. The antibacterial activities of these soaps were determined by the diffusion method of disks in agar medium. Results and Discussion: Statistical analysis of zones of inhibition showed that S. epidermidis, P. aeruginosa and Enterobacter Spp. were sensitive only to traditional oil-based soaps, which are Sage, Rose Mary and Cedar. In addition, S. aureus showed sensitivity to soaps comprised of essential oils as well as antibacterial synthetic soap, Dettol and Lifebuoy. On the other hand, E. coli showed resistance to all soaps. Soaps comprised of natural essential oils have shown antibacterial activity superior to so-called “Antibacterial” soaps. Conclusion: Based on this study, we can say that the use of soaps with essential oils might be the best option due to their organic origin as well as their antibacterial proved activities.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mohammed Dalli ◽  
Salah-eddine Azizi ◽  
Hind Benouda ◽  
Ali Azghar ◽  
Maroua Tahri ◽  
...  

Nigella sativa L. (NS) and its volatile compounds are well known for their broad spectrum of effects. This study aimed to investigate the variability of the chemical composition and the in vitro antibacterial activity of five essential oils (Eos) originated from Morocco, Saudi Arabia, Syria, India, and France. These five samples were grown under different edaphic and climatic conditions. The agar diffusion method and microdilution method in 96-well plates were used to test the sensitivity of multidrug-resistant strains clinically isolated from patients (methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii), for the determination of the minimum inhibitory concentration and bactericidal concentration. Among all the investigated Eos, the monoterpenes were highly present in the chemical composition. Moroccan, Saudi Arabian, and Syrian seeds were characterized by the presence α-phellandrene (20.03–30.54%), β-cymene (12.31–23.82 %), and 4−caranol (9.77–14.27%). The Indian seeds were rich with 4-caranol (18.81%), β-cymene (14.22%), α-phellandrene (10.58%), and β-chamigrene (9.54%), while France NS was rich with estragole (20.22%) and D-limonene (14.63%). The minimum inhibitory (MIC) and bactericidal concentration (MBC) obtained for the four Eos (with the exception of France because of the low yield) tested were ranging from 3 to 40 μl/ml. Gram-positive (+) bacteria were slightly sensitive to the Eos tested than the Gram-negative (−) bacteria. The results of this study showed that the Eos of NS seeds show interesting antibacterial activity which could be associated to the existence of different bioactive compounds. Indeed, these compounds can be used for preventive or curative purposes in the face of the noncontrolled emergence of resistance to antibiotics.


2012 ◽  
Vol 64 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Irena Novakovic ◽  
Jelena Penjisevic ◽  
V. Sukalovic ◽  
Deana Andric ◽  
G. Roglic ◽  
...  

The derivatives of cinnamic acid and N-arylpiperazine show antibacterial activity. In this work the potential synergistic effect of cinnamyl derivatives of arylpiperazine in selected bacteria was investigated. The antibacterial activities of the derivatives were evaluated against Gram-positive bacteria: Staphylococcus aureus, Streptosporangium longisporum, Sarcina lutea, Micrococcus flavus, Clostridium sporogenes and Bacillus subtilis and Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis and Proteus vulgaris by the disc diffusion method. The minimum inhibitory concentration (MIC) against the selected bacteria was determined for all compounds that showed activity in the disc diffusion method. The majority of the investigated compounds displayed in vitro antibacterial activity. The effect of the type and structure of the substituent on the aromatic ring on the antibacterial activity is discussed. It was found that two derivatives expressed activity toward S. longisporum and P. aeruginosa that was almost as strong as that of amikacin.


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