Kinetics Analysis and Susceptibility Coefficient of the Pathogenic Bacteria by Titanium Dioxide and Zinc Oxide Nanoparticles

Purpose: The increase of bacterial resistance to common antibacterial agents is one of the major problems of health care systems and hospital infection control programs. In this study, antimicrobial activity of titanium dioxide (TiO2 ) and zinc oxide (ZnO) nanoparticles (NPs) was investigated against E. coli, Salmonella enteritidis, Listeria monocytogenes, and Staphylococcus aureus pathogenic bacteria by determining sensitivity coefficient and kinetics of bacterial death. Methods: Antimicrobial tests were performed with ~106 CFU/mL of each bacterium at baseline. At first, minimum inhibitory concentration (MIC) was concluded by the dilution method and then, death kinetic and susceptibility coefficient of NPs suspensions was determined at 0 to 360 min. treatment time. Results: The results of this study revealed that, the highest susceptibility was observed for L. monocytogenes (Z=0.025 mL/μg) to TiO2 NPs, whereas the lowest susceptibility was obtained in the reaction of ZnO NPs with S. enteritidis (Z=0.0033 mL/μg). The process of bacterial death in NPs suspension was assumed to follow first-degree kinetic and the survival ratio of bacteria decreased by the increase in treatment time. An increase in the concentration of NPs was seen to enhance the bactericidal action. Conclusion: Results showed that L. monocytogenes had higher sensitivity compared to S. enteritidis. The results of this study also demonstrated that TiO2 NPs have a strong antimicrobial effect in comparison with ZnO NPs and it could be employed to aid the control of pathogenic bacteria.

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Synthesis of Zinc Oxide, Titanium Dioxide and Magnesium Dioxide Nanoparticles and Their Prospective in Pharmaceutical and Biotechnological Applications

Journal of Biomedical Research & Environmental Sciences ◽

10.37871/jbres1180 ◽

2021 ◽

Vol 2 (1) ◽

pp. 011-020

Author(s):

Abhinav Shrivastava ◽

Ravi Kant Singh ◽

Pankaj Kumar Tyagi ◽

Dilip Gore

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Titanium Dioxide ◽

Small Molecules ◽

Safety Assessment ◽

Biotechnological Applications ◽

Zno Nps ◽

Tio2 Nps ◽

Titanium Dioxide Nanoparticle ◽

Oxide Nanoparticle

The use of nanoparticles for the therapeutic purpose is gaining pronounced importance. In the last two decades, a number of nanomedicines received regulatory approval and several showed promises through clinical trials. In this content, it is important to synthesize nanoparticles from various sources and to check its efficiency, especially its antibacterial activity. In today’s scenario number nanomedicines are proving useful to control multidrug resistance and since the mechanism of action of nanoparticles is totally different from the small molecules like antibiotics it obviates the chances of drug resistance. In this review, we discussed three metal-based nanoparticles prepared from various reducing sources namely Zinc Oxide Nanoparticle (ZnO NPs), Titanium Dioxide Nanoparticle (TiO2 NPs) and Magnesium Dioxide Nanoparticle (MnO2 NPs). The focus also made towards the safety assessment of the several nanoparticles. In addition, the exact interaction of the nanoparticles with the bacterial cell surface and the resultant changes also been highlighted. The review put forward the sources, method, and antibacterial success of these nanoparticles so that future nanomedicines could be put forward.

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Insecticidal Effect of Zinc Oxide and Titanium Dioxide Nanoparticles against Bactericera cockerelli Sulc. (Hemiptera: Triozidae) on Tomato Solanum lycopersicum

Agronomy ◽

10.3390/agronomy11081460 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1460

Author(s):

José A. Gutiérrez-Ramírez ◽

Rebeca Betancourt-Galindo ◽

Luis A. Aguirre-Uribe ◽

Ernesto Cerna-Chávez ◽

Alberto Sandoval-Rangel ◽

...

Keyword(s):

Zinc Oxide ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Block Design ◽

Field Tests ◽

Laboratory Research ◽

Bactericera Cockerelli ◽

Zno Nps ◽

Tio2 Nps ◽

Insecticidal Effect

The use of nanoparticles (NPs) has generated an alternative pest control. The objective was to evaluate the insecticidal effect of zinc oxide nanoparticles (ZnO NPs), titanium dioxide nanoparticles (TiO2 NPs), and their combination on Bactericera cockerelli (Hemiptera: Triozidae) second-stage nymphs under laboratory and greenhouse conditions in tomato. The laboratory research was carried out with the leaf immersion bioassay method under a complete randomized design, and in the greenhouse by direct plant spraying under a randomized block design; in both designs, a control without NPs was added. Mortality was recorded every 24 h for 4 days. Both NPs in the laboratory and greenhouse showed toxicity to B. cockerelli nymphs. Results in the laboratory showed that NPs significantly caused increased mortality of 88, 99, and 100% 96 h after treatment of ZnO NPs, TiO2 NPs, and their combinations, at 1000, 100, and 250 ppm, respectively. Direct spray of plants in the greenhouse showed low mortality with 27, 32, and 23% after 96 h of ZnO NPs, TiO2 NPs, and their combinations, at 3000, 500, and 250 ppm, respectively. These results on B. cockerelli control seem promising. Nanoparticles as insecticides are a novel strategy, however, further investigation is required in field tests to obtain suitable efficacy for use in a pest management system.

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Biosynthesized Zinc Oxide Nanoparticles Disrupt Established Biofilms of Pathogenic Bacteria

Applied Sciences ◽

10.3390/app12020710 ◽

2022 ◽

Vol 12 (2) ◽

pp. 710

Author(s):

Fohad Mabood Husain ◽

Faizan Abul Qais ◽

Iqbal Ahmad ◽

Mohammed Jamal Hakeem ◽

Mohammad Hassan Baig ◽

...

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Pathogenic Bacteria ◽

Oxide Nanoparticles ◽

Antibiofilm Activity ◽

Gram Negative Bacteria ◽

Zno Nps ◽

Gram Positive ◽

Gram Negative ◽

E Coli

Global emergence and persistence of the multidrug-resistant microbes have created a new problem for management of diseases associated with infections. The development of antimicrobial resistance is mainly due to the sub-judicious and unprescribed used of antimicrobials both in healthcare and the environment. Biofilms are important due to their role in microbial infections and hence are considered a novel target in discovery of new antibacterial or antibiofilm agents. In this article, zinc oxide nanoparticles (ZnO-NPs) were prepared using extract of Plumbago zeylanica. ZnO-NPs were characterized and then their antibiofilm activity was tested against Gram-positive and Gram-negative bacteria. The ZnO-NPs were polydispersed, and the average size was obtained as 24.62 nm. The presence of many functional groups indicated that phytocompounds of P. zeylanica were responsible for the synthesis, capping, and stabilization of ZnO-NPs. Synthesized NPs inhibited the biofilm formation of E. coli, S. aureus, and P. aeruginosa by 62.80%, 71.57%, and 77.69%, respectively. Likewise, concentration-dependent inhibition of the EPS production was recorded in all test bacteria. Microscopic examination of the biofilms revealed that ZnO-NPs reduced the bacterial colonization on solid support and altered the architecture of the biofilms. ZnO-NPs also remarkably eradicated the preformed biofilms of the test bacteria up to 52.69%, 59.79%, and 67.22% recorded for E. coli, S. aureus, P. aeruginosa, respectively. The findings reveal the ability of green synthesized zinc oxide nanoparticles to inhibit, as well as eradicate, the biofilms of Gram-positive and Gram-negative bacteria.

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Potential Bacterial Symbion of Sea Urchin As a Multi-Drug Resistant (MDR) Antibacterial Agent Against Staphylococcus aureus and Escherichia Coli Bacteria

ILMU KELAUTAN Indonesian Journal of Marine Sciences ◽

10.14710/ik.ijms.23.3.131-136 ◽

2018 ◽

Vol 23 (3) ◽

pp. 131

Author(s):

Suzana Kristy Satriani Fofied ◽

Agus Sabdono ◽

Diah Permata Wijayanti

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Sea Urchin ◽

Pathogenic Bacteria ◽

Bacterial Resistance ◽

Symbiotic Bacteria ◽

Dilution Method ◽

Drug Resistant ◽

Escherichia Coli Bacteria

Staphylococcus aureus and Escherichia coli are pathogenic bacteria agent of many human diseases. Those bacteria infect in various levels and also been antibiotic resistants. Bacterial resistance has become a serious global problem. The purposes of this study were to isolate and identify the symbiotic bacteria of the Sea Urchin that have an antibacterial activity of the Strain Multi-Drug Resistant (MDR) against Staphylococcus aureus and Escherichia coli. Sea Urchin was taken from Panjang island, Jepara Indonesia at 2-3 m depth. The symbiotic bacteria were isolated from Sea Urchin by using dilution method and spread plate method. Phenotypic characteristics was observed on colony shape, color and texture of growing bacteria. While the streak method was used to purify bacterial symbion. The antibacterial activity test was performed using overlay method. The results showed that 3 out of 37 isolates have antibacterial activity against Staphylococcus aureus and Escherichia coli. The BB.03.35 isolate was selected for molecular identification due to the largest inhibitory zone diameter. The sequence of this bacterium showed 97% homology and closely related to Pseudoalteromonas flavipulchra.

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IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.28808 ◽

2019 ◽

Vol 12 (1) ◽

pp. 200

Author(s):

Vinoy Jacob ◽

Rajiv P

Keyword(s):

Antimicrobial Activity ◽

Zinc Oxide ◽

Pathogenic Bacteria ◽

Curcuma Longa ◽

Reducing Power ◽

Zno Nps ◽

Natural Medicine ◽

Vitro Analysis ◽

In Vitro Analysis

Objective: Curcuma longa is a known natural medicine for inflammation from ancient times. It has a low absorption rate and poor solubility. Hence, it is used for the green synthesis of nanoparticles. Zinc oxide nanoparticle (ZnO NPs) is famous nanoparticles which are economical, less toxic, and brilliantly biocompatible. They have potential biomedical properties, mainly anticancer, antidiabetic, and antimicrobial.Methods: The present study was designed to investigate in vitro analysis of the antimicrobial activity against pathogenic bacteria and fungi and its ability to scavenge reactive oxygen radicals of ZnO NPs.Results and Conclusion: The results indicated that ZnO NPs produced from C. longa had higher antimicrobial activity against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans. Therefore, we suggest that ZnO NPs can be used as the antimicrobial agent. It is a good scavenger of superoxide radical, nitric oxide, and hydrogen peroxide and has reducing power, which is greater than ascorbic acid at a higher concentration.

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Antibacterial Action and Target Mechanisms of Zinc Oxide Nanoparticles Against Bacterial Pathogens

10.21203/rs.3.rs-1054432/v1 ◽

2021 ◽

Author(s):

Carolina Rosai Mendes ◽

Guilherme Dilarri ◽

Carolina Froes Forsan ◽

Vinícius de Moraes Ruy Sapata ◽

Paulo Renato Matos Lopes ◽

...

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Bacterial Resistance ◽

Membrane Integrity ◽

Antimicrobial Properties ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

Zno Nps ◽

Z Ring ◽

Ft Ir

Abstract Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. The study characterized ZnO NPs using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of clinically bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa was evaluated by REMA after exposure to the ZnO NP at concentrations from 0.2 to 1.4 mM. Sensitivity was achieved at 0.6 mM for the Gram-negatives and 1.0 mM for Gram-positives cells. The effect of ZnO NPs on the membrane integrity and in the interference of cell division was investigated by its effect on the divisional ring, through fluorescence microscopy assays using B. subtilis (amy::pspac-ftsZ-gfpmut1) expressing FtsZ-GFP. Results showed that ZnO NPs did not interfere with the assembly of the divisional Z-ring. However, 70% of the cells showed damage in the cytoplasmic membrane after 15 min of exposure to the ZnO NPs. Electrostatic forces, production of Zn2+ ions, generation of reactive oxygen species were described as pathways of bactericidal action by ZnO. Thus, understanding bactericidal MOA can produce predictive models to prevent bacterial resistance and lead to further research.

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Foliar Application of Low Concentrations of Titanium Dioxide and Zinc Oxide Nanoparticles to the Common Sunflower under Field Conditions

Nanomaterials ◽

10.3390/nano10081619 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1619 ◽

Cited By ~ 1

Author(s):

Marek Kolenčík ◽

Dávid Ernst ◽

Martin Urík ◽

Ľuba Ďurišová ◽

Marek Bujdoš ◽

...

Keyword(s):

Zinc Oxide ◽

Titanium Dioxide ◽

Foliar Application ◽

Development Stage ◽

Field Conditions ◽

Plant Production ◽

Flower Bud ◽

Zno Nps ◽

Early Maturation ◽

Experimental Treatments

Nano-fertilisers have only recently been introduced to intensify plant production, and there still remains inadequate scientific knowledge on their plant-related effects. This paper therefore compares the effects of two nano-fertilisers on common sunflower production under field conditions. The benefits arising from the foliar application of micronutrient-based zinc oxide fertiliser were compared with those from the titanium dioxide plant-growth enhancer. Both the zinc oxide (ZnO) and titanium dioxide (TiO2) were delivered by foliar application in nano-size at a concentration of 2.6 mg·L−1. The foliar-applied nanoparticles (NPs) had good crystallinity and a mean size distribution under 30 nm. There were significant differences between these two experimental treatments in the leaf surfaces’ trichomes diversity, ratio, width, and length at the flower-bud development stage. Somewhat surprisingly, our results established that the ZnO-NPs treatment induced generally better sunflower physiological responses, while the TiO2-NPs primarily affected quantitative and nutritional parameters such as oil content and changed sunflower physiology to early maturation. There were no differences detected in titanium or zinc translocation or accumulation in the fully ripe sunflower seeds compared to the experimental controls, and our positive results therefore encourage further nano-fertiliser research.

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The Effectiveness of Nanocomposite Films Against Gram-Positive and Gram-Negative Foodborne Pathogenic Bacteria

Basic and Applied Sciences - Scientific Journal of King Faisal University ◽

10.37575/b/sci/210049 ◽

2021 ◽

pp. 1-6

Author(s):

Khaled Saif-Aldin ◽

Sahar Al-Hariri ◽

Adnan Nizam ◽

Obaida Alhajali

Keyword(s):

Zinc Oxide ◽

Food Packaging ◽

Pathogenic Bacteria ◽

Nanocomposite Films ◽

Zno Nps ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Nano Zinc Oxide ◽

Nano Zinc

In this research, nanocomposites consisting of a mixture of linear low-density polyethylene polymer (LLDPE) and zinc oxide nanoparticles (ZnO-NPs) were prepared. The films of the composite material were formed with five weight ratios (0.25, 0.5, 1, 2.5, and 5wt%) in addition to pure LLDPE, intended to investigate the role of nanomaterials in improving the performance of some properties of LLDPE polymer such as increasing the shelf life of food products and protecting the consumer from pathogenic germs in food packaging applications. The efficacy was evaluated against pathogenic bacteria, Escherichia coli and Staphylococcus aureus, through the standard "ISO 22196". The test results confirm that the nanocomposite films containing 0.5wt% or more of nano-zinc oxide have bacteriostatic activity. This activity increases with the increase of ZnO-NPs in the LLDPE polymeric phase, and the highest antibacterial effect was in the nanocomposite films of 5wt%. It was found that gram-positive bacteria were more sensitive to ZnO-NPs than gram-negative bacteria and that these nanocomposite films can provide a safe way to preserve food without the need for food processing.

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IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.28808 ◽

2019 ◽

Vol 12 (1) ◽

pp. 200 ◽

Cited By ~ 3

Author(s):

Vinoy Jacob ◽

Rajiv P

Keyword(s):

Antimicrobial Activity ◽

Zinc Oxide ◽

Pathogenic Bacteria ◽

Curcuma Longa ◽

Reducing Power ◽

Zno Nps ◽

Natural Medicine ◽

Vitro Analysis ◽

In Vitro Analysis

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Investigations on synergistic and antioxidant actions of medicinal plant based biosynthesis of Zinc Oxide Nanoparticles against E.coli and K. pneumonia bacteria

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207324666210302102111 ◽

2021 ◽

Vol 24 ◽

Author(s):

Farzana Rashid ◽

Iqra Pervaiz ◽

Husna Malik ◽

Zakia Kanwal ◽

Muhammad Rafique ◽

...

Keyword(s):

Antioxidant Activity ◽

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Bacterial Resistance ◽

Synergistic Effects ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Dpph Method ◽

Multiple Drugs

Introduction: Bacterial resistance to multiple drugs is increasing at an alarming rate in current era and nanotechnology is one of the effective and novel approaches to overcome drug resistance. Methods: Zinc Oxide Nanoparticles (ZnO NPs) has stronger antibacterial activity and is regarded as bio-safe nanomaterial. The aim of present study is to synthesize the ZnO NPs using Aloe vera leaves extract and to investigate the synergistic effects and antioxidant actions of bio-synthesized ZnO NPs against gram negative bacteria E.coli and K. pneumoniae. The synergistic effect of β-lactam antibiotics (meropenem and ciprofloxacin) was tested along with ZnO NPs by using Kirby’s disc diffusion assay. The antioxidant activity was investigated by α, α-diphenyl-β-picrylhydrazyl (DPPH) method. Results: Results revealed that the antibacterial activity of the selected antibiotics was much enhanced by ZnO NPs than the antibiotics alone. The resistant antibiotic (ciprofloxacin) became sensitive when combined with ZnO NPs. The antioxidant activity reveals that biosynthesized ZnO NPs possess significantly higher (p<0.05) antioxidant activity (77%). Conclusion: The findings reveal that biosynthesized ZnO NPs have much more eco-friendly approach. It can act as a strong potentiator of β-lactam antibiotics and put forward the possibility to use them effectively in targeted drug delivery, pharmaceuticals and biomedical fields.

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