scholarly journals The usage of THz radiation of nitrogen oxide occurrence in blood antioxidant characteristics correction and lipid peroxidation process under stress conditions

2013 ◽  
Vol 2 (3) ◽  
pp. 0303
Author(s):  
Vyacheslav F. Kirichuk ◽  
Alexander A. Tsymbal
Keyword(s):  
Lipid Peroxidation ◽  
Nitrogen Oxide ◽  
Stress Conditions ◽  
Thz Radiation ◽  
Lipid Peroxidation Process ◽  
Antioxidant Characteristics

scholarly journals Withania somnifera promotes stress resistant activity in Drosophila melanogaster

2018 ◽  
Vol 8 (6-s) ◽  
pp. 83-88
Author(s):  
B.P. Renuka Prasad ◽  
J.S. Ashadevi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Drosophila Melanogaster ◽  
Stress Tolerance ◽  
Withania Somnifera ◽  
Stress Conditions ◽  
High Dose ◽  
Oxidative Stresses ◽  
Paraquat Toxicity ◽  
Emotional Strain

Stress is a state of mental or emotional strain of an individual.  In recent years nutritional antioxidants study have gain more attention in minimizing the stress like oxidative stress. The stress resistant ability in an organism can be increased by the supplementation of herbal resources. However, few plant extracts are known to have stress resistant ability and increases the tolerance capacity. Plants containing high antioxidant and other bioactive compounds promote tolerance capacity. An antioxidant rich plant has been proved to decreases the   lipid peroxidation. Here, we investigated the potential protective effect of ethanolic extract of Withania somnifera (WS), against Paraquat toxicity on stress tolerance capacity using Drosophila melanogaster. Wild-type fruit flies of Oregon-K strain were fed with standard food media with 1mg/ml and 10 mg/ml of WS.  The oxidative stress was induced by exposing the extract supplemented flies to Paraquat (20 mM).  The stress tolerance capacity of flies was measured by subjecting to desiccation and oxidative stresses. Further, locomotor activity, lipid peroxidation were also studied along with the quantification of triglycerides, glycogen in WS fed flies under stress conditions. Our result reveals that PQ induced WS fed flies showed greater survivability, better locomotor ability when compared to PQ induced flies.WS fed flies increases about 73.55% of resistance ability under oxidative conditions and increased by 59.15% under desiccation than PQ induced flies. WS was more effective in protecting against Paraquat toxicity. The flies fed with high dose of WS (10mg/ml) showed greater improvement of the tolerance ability when subjected to desiccation and oxidative stresses. Further, the data on biochemical analysis reveals that lipid peroxidation activities were found to be significantly low and the triglyceride as well as glycogen quantities were found to be significantly high in WS fed flies compare to –ve control under both desiccation and oxidative stress conditions.  Together, these findings suggest that WS promotes stress resistant ability by modulating metabolism and reducing oxidative damage. Keywords: Drosophila melanogaster, Withania somnifera, Oxidative stress assay, Desiccation Assay, Negative Geotaxis, 

scholarly journals Neuroprotective action of aspirin on Paraquat intoxication in on Drosophila melanogaster

2021 ◽  
Vol 10 (4) ◽  
pp. e30710414179
Author(s):  
Mayslla Keylla Brito do Carmo ◽  
Mayara Oliveira Vidotto Figueiredo ◽  
Joyce Marinho de Souza ◽  
Anderson Oliveira Souza ◽  
Carlos Antonio Couto Lima
Keyword(s):  
Lipid Peroxidation ◽  
Drosophila Melanogaster ◽  
Motor Performance ◽  
Ache Activity ◽  
Marked Decrease ◽  
Wild Type ◽  
Neuroprotective Effects ◽  
The Central Nervous System ◽  
Antioxidant Characteristics ◽  
Neuroprotective Action

Acetylsalicylic acid or aspirin is the most widely used drug globally for its anti-inflammatory characteristics, although little is known about its actions on the central nervous system (CNS). We investigated aspirin's potential neuroprotective effects against paraquat-induced neurotoxicity (PQ) in the present study. Adult male wild-type flies were exposed to a diet containing PQ (3 mM) and/or aspirin (1 μM; 5 μM; 10 μM). Flies fed with PQ reduced locomotion and increased mortality. PQ-induced neurotoxicity has also been associated with a marked decrease in acetylcholinesterase (AChE) activity and lipid peroxidation. Co-exposure to aspirin (5 μM) increased survival, improved motor performance, increased AChE activity, and decreased lipid peroxidation. Our results suggest aspirin's neuroprotective effects, probably due to its lysosomal action and antioxidant characteristics. Thus, we demonstrate that the Drosophila melanogaster model can elucidate basic aspirin mechanisms to assist the evaluations carried out in higher animals to minimize the neurodegenerative effects caused by diseases such as Parkinson's and Alzheimer's.

Catalase prevents prostanoid release and lung lipid peroxidation after endotoxemia in sheep

1988 ◽  
Vol 65 (3) ◽  
pp. 1210-1216 ◽  
Author(s):  
A. Seekamp ◽  
C. Lalonde ◽  
D. G. Zhu ◽  
R. Demling
Keyword(s):  
Lipid Peroxidation ◽  
Water Content ◽  
Fold Increase ◽  
Conjugated Dienes ◽  
Base Line ◽  
Lipid Peroxidation Process ◽  
Adult Sheep ◽  
Lung Lipid ◽  
Arterial Plasma ◽  
Lung Lymph

We determined the effect of H2O2 on both the physiological and biochemical lung changes seen in the adult sheep after endotoxin. Fourteen unanesthetized adult sheep with chronic lung lymph fistula were given Escherichia coli endotoxin (1 microgram/kg) over 30 min. Seven sheep were given catalase (32,500 U/kg body wt) as an intravenous bolus 30 min before endotoxin. Four sheep were given catalase alone. Oxidant lung changes were measured using arterial plasma conjugated dienes and lung tissue malondialdehyde (MDA) content, both reflecting the lipid peroxidation process. Animals were killed 5 h after endotoxin. We found that endotoxin alone caused an early increase in pulmonary arterial pressure lung lymph flow (QL), plasma thromboxane B2, 6-keto-prostaglandin F1 alpha, and plasma conjugated dienes. A decrease in cardiac output and arterial PO2 was also seen. A three- to four-fold increase in protein-rich QL was noted at 3-4 h as well as a continued increase in arterial conjugated dienes. Lung MDA and water content were also significantly increased from base line. Catalase pretreatment significantly attenuated both the physiological changes and the prostanoid and conjugated diene release. Lung MDA and water content also remained at base line. We conclude that H2O2 plays a major role in endotoxin-induced lung injury as well as the resulting lipid peroxidation process.

scholarly journals Effect of exogenous alpha-tocopherol on physio-biochemical attributes and agronomic performance of lentil (Lens culinaris Medik.) under drought stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0248200
Author(s):  
Wadood Shah ◽  
Sami Ullah ◽  
Sajjad Ali ◽  
Muhammad Idrees ◽  
Muhammad Nauman Khan ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Growth Rate ◽  
Drought Stress ◽  
Leaf Area ◽  
Glycine Betaine ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Stress Conditions ◽  
Area Index ◽  
Induced Stress

Water being a vital part of cell protoplasm plays a significant role in sustaining life on earth; however, drastic changes in climatic conditions lead to limiting the availability of water and causing other environmental adversities. α-tocopherol being a powerful antioxidant, protects lipid membranes from the drastic effects of oxidative stress by deactivating singlet oxygen, reducing superoxide radicals, and terminating lipid peroxidation by reducing fatty acyl peroxy radicals under drought stress conditions. A pot experiment was conducted and two groups of lentil cultivar (Punjab-2009) were exposed to 20 and 25 days of drought induced stress by restricting the availability of water after 60th day of germination. Both of the groups were sprinkled with α-tocopherol 100, 200 and 300 mg/L. Induced water deficit stress conditions caused a pronounced decline in growth parameters including absolute growth rate (AGR), leaf area index (LAI), leaf area ratio (LAR), root shoot ratio (RSR), relative growth rate (RGR), chlorophyll a, b, total chlorophyll content, carotenoids, and soluble protein content (SPC) which were significantly enhanced by exogenously applied α-tocopherol. Moreover, a significant increase was reported in total proline content (TPC), soluble sugar content (SSC), glycine betaine (GB) content, endogenous tocopherol levels, ascorbate peroxidase (APX), catalase (CAT) peroxidase (POD) and superoxide dismutase (SOD) activities. On the contrary, exogenously applied α-tocopherol significantly reduced the concentrations of malondialdehyde (MDA) and hydrogen peroxide (H2O2). In conclusion, it was confirmed that exogenous application of α-tocopherol under drought induced stress regimes resulted in membrane protection by inhibiting lipid peroxidation, enhancing the activities of antioxidative enzymes (APX, CAT, POD, and SOD) and accumulation of osmolytes such as glycine betaine, proline and sugar. Consequently, modulating different growth, physiological and biochemical attributes.

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