scholarly journals Effect of Seed Maturity and Storage Duration on Germination of Sambiloto (Andrographis paniculata (Burm.f.) Nees)

Author(s):  
Solikin Solikin ◽  
Siti Nurfadilah

<p>Seed  maturity  and its storage are one of problems  on  propagation and developing of medicinal   plants such as sambiloto (<em>Andrographis paniculata </em>(Burm.f.) ex Nees). The research  aimed  to determine effects of seed maturity and storage duration on seed germination of  sambiloto was conducted in a green house of  Purwodadi Botanical Garden from  November 2014 to November 2015.  The experiment done by completely randomised design  with two  treatments  namely seed  maturity  and  storage duration. The seed was classified into immature seed (0.061 g/100 seeds), semimature seed (0.113 g/100 seeds) and mature seed (0.166 g/100 seeds). The seed’s  storage duration was classified into seed was not stored, seed was stored  for six months and seed was stored for  twelve months. Each treatment combination was replicated five times. The results showed that there were significant interactions between the treatments on percentage and rate of seed germination.  The highest percentage of seed germination was the treatment of mature seed and stored twelve months (98.50%). On the contrast, the lowest seed germination percentage was the treatment of immature seed and stored twelve months (4.25 %). The fastest seed  germination rate was the treatment of mature seed and stored  six months (3.88 days), whereas the slowest seed germination rate was the treatment of immature  seed and without  stored (28.58 days). This study is expected to be applied to improve genetic and cultivation of medicinal plant as well as increasing plant growth and yield.</p>

2020 ◽  
Vol 13 (1) ◽  
pp. 21
Author(s):  
DEVI RUSMIN ◽  
MELATI, S MELATI, S ◽  
WAHYUNI WAHYUN ◽  
SUKARMAN SUKARMAN

ABSTRAK<br />Salah satu faktor yang berpengaruh terhadap viabilitas benih<br />sambiloto (Andrographis paniculata Nees) adalah waktu panen.<br />Berdasarkan permasalahan tersebut kegiatan ini dilakukan dengan tujuan<br />untuk mengetahui pengaruh umur panen terhadap viabilitas benih serta<br />hubungannya dengan produksi terna sambiloto. Percobaan dilakukan di<br />KP. Cimanggu dan Laboratorium, Balai Penelitian Tanaman Obat dan<br />Aromatik, dari Maret 2005 – Maret 2006. Percobaan disusun dalam<br />rancangan acak kelompok (RAK) dengan 10 perlakuan stadia umur<br />panen dan 4 ulangan. Stadia umur panen yang diuji yaitu 18, 21, 22, 23,<br />24, 25, 26, 27, 28, dan 29 hari setelah antesis (HSA). Variabel yang<br />diamati yaitu mutu benih (daya berkecambah benih, kecepatan<br />berkecambah), pertumbuhan tanaman (tinggi tanaman, jumlah cabang),<br />serta produksi terna (bobot basah tanaman, bobot kering daun, dan bobot<br />kering batang). Hasil penelitian menunjukkan bahwa: (1) Umur panen<br />benih berpengaruh terhadap daya berkecambah benih dan kecepatan<br />berkecambah benih sambiloto; daya berkecambah dan kecepatan<br />berkecambah tertinggi didapatkan pada umur panen benih 22 dan 21 HSA<br />(67,00 dan 55,00)%; sedangkan daya berkecambah yang terendah<br />diperoleh pada umur panen 18 HSA (23,50)%, (2) Umur panen benih<br />berpengaruh terhadap tinggi tanaman dan jumlah cabang pada tanaman<br />umur 1 bulan. Tinggi tanaman dan jumlah cabang tertinggi berturut-turut<br />didapatkan pada perlakuan umur panen benih 27 dan 26 HSA (39, 63<br />dan 36,58 cm serta 16,71 dan 16,61 buah); dan (3) Umur panen benih<br />berpengaruh terhadap produksi terna (bobot basah tanaman, bobot kering<br />daun, bobot kering batang) pada umur 3 bulan. Bobot basah tanaman,<br />bobot kering daun, serta bobot kering batang tertinggi didapatkan pada<br />perlakuan umur panen benih 27 HSA (291,25, 28, 27 dan 28,86)g. Bobot<br />basah tanaman, bobot kering daun, serta bobot kering batang terendah<br />didapatkan pada perlakuan 18 HSA (217,09, 22,10 dan 20,24)g. Umur<br />panen benih tidak berpengaruh terhadap jumlah cabang pada umur 3<br />bulan.<br />Kata kunci : Sambiloto, Andrographis paniculata Nees, umur panen,<br />viabilitas, produksi, Jawa Barat<br />ABSTRACT<br />The influence of harvesting time on the seed viability and<br />the  relationship  with  herb  yield  of  king  bitter<br />(Andrographis paniculata Nees)<br />One of the main factors influencing the viability of king bitter<br />(Andrographis paniculata Nees) is appropriate harvesting time. Based on<br />this problem a research was conducted to study the relationship between<br />seed maturity and seed viability, and herb yield of king bitter. The research<br />was conducted at Cimanggu Experimental Station and in the laboratory of<br />Indonesian Medicinal and Aromatic Crops Research Institute (IMACRI)<br />from March 2005 to March 2006. The experiment was arranged in a<br />randomized block design with 10 seed maturity stages and 4 replications.<br />Maturity seed tested was 18, 21, 22, 23, 24, 25, 26, 27, 28 and 29 days<br />after anthesis. The observations were made on seed quality (percentage of<br />seed germination, and rate of seed germination), plant growth (plant<br />height, number of branches) and herb yield (fresh herb weight, leaf dry<br />weight, and stem dry weight). The results of experiment indicated that (1)<br />seed maturity affected seed germination, and rate of seed germination of<br />king bitter; the highest seed germination and germination rate were<br />achieved by seeds harvested at 21 and 22 days after anthesis, (2) stage of<br />seed harvesting affected on the plant height and number of branches at 1<br />month after planting. The highest plant height and number of branches<br />were found on the treatments of seeds harvested at 26 and 27 days after<br />anthesis, they were 39.63 and 36.58 cm and 16.71 and 16.61; (3) seed<br />maturity also affected herb production, such as wet weight of plants, dry<br />weight of leaf and dry weight of steam at 3 moths after planting. Wet<br />weight of plant, dry weight of leaf and dry weight of stem were achieved<br />at the treatments of seeds harvested at 27 days after anthesis, they were<br />291.25, 28.27 and 28.86 g. The lowest of wet of plant weight, dry weight<br />of leaf and dry weight of stem were found on the seeds harvested at 18<br />days after anthesis. They were 217.09, 22.10 and 20.24 g. Moreover, the<br />stage of harvesting did not influence the number of branches at 3 months<br />after planting.<br />Key words: King bitter, Andrographis paniculata Nees, seed maturity,<br />viability, production, West Java


2014 ◽  
Vol 92 (2) ◽  
pp. 281 ◽  
Author(s):  
Martha Cervantes ◽  
Eliane Ceccon ◽  
Consuelo Bonfil

<p><span style="font-size: medium;"><span style="font-family: Times New Roman;">Studies on propagation of trees of Tropical Dry Forests are scarce in Mexico, besides, the provenance of seeds used in reforestation programs is generally unknown or poorly addressed. Knowledge on seed germination patterns of different provenances, and how they change through time, is useful to identify adequate sources of seeds and to develop seed collection and storage programs under the low-tech conditions prevailing in most rural nurseries. We evaluated seed size variation and germination of stored seeds from three different provenances per species in <em>Acacia bilimekii</em>, <em>Haematoxylum brasiletto</em>, <em>Lysiloma acapulcense</em>, and<em> L. divaricatum</em>.<em> </em>Seeds were collected in four sites in the Tropical Dry Forest of Morelos, Mexico, and were stored at room temperature; seed size was estimated through the volume of 75 seeds per provenance/species. Seed germination tests were made periodically from six to 24 months after storage and the effects of provenance and storage time on germination were analyzed using Anovas. There were significant differences in seed size among provenances in all species, while the effect of provenance on germination rate was significant in three of them. Germination rate changed with storage time among species and provenances. After 24 months, germination capacity was still ≥ 50% in all provenances of the two <em>Lysiloma</em> species, but in <em>A.</em> <em>bilimekii</em> there were large differences among provenances. Mean germination capacity was low in <em>H. brasiletto</em> after six months. More research on seed germination and storage of a larger set of species and provenances is needed to restore the Mexican tropical dry forests. </span></span></p>


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 618d-618
Author(s):  
Chin-Chang Chu ◽  
Kenneth W. Mudge

Seeds from mature seed pods of Cypripedium calceolus var. parviflorum were germinated on 1/4 MSMO (Sigma) + 100ml/l coconut water + 1% sucrose +/- 8g/l agar (pH 6.0), and with or without prechilling at 5C for 8 weeks. Protocorm with apex (stage 3) was use as an index of germination. Seeds sown on agar medium withou chilling treatment resulted in a 40% germination rate in 120 days but the germination was very uneven. Seeds germinated on agar medium with prechilling developed more synchronously with 92% germination in 60 days (ie. about 120 days after sowing). Suspension culture of seeds without prechilling resulted in 85% germination after 90 days. The synchronization of seed germination in suspension culture was intermediate between that on agar with and without prechilling. Protocorms germinated in suspension culture appeared morphologically identical to those germinated on agar medium. All stage 3 protocorms developed further on the same agar medium in darkness. Both agar and suspension culture in media containing coconut water provided reliable seed germination methods for this orchid species.


2017 ◽  
Vol 3 (4) ◽  
pp. 233
Author(s):  
Sri Ulie Rahmawati ◽  
Arief Noor Rachmadiyanto

<p class="Els-body-text"><em>Markhamia stipulata </em>(Wall.) Seem. (Bignoniaceae) is one of collections in Bogor Botanical Garden that potential as a medicine. Information about germination of these species were not known yet. This research was aimed to examine the effect of light color on germination process of <em>Markhamia stipulata</em> (Wall.) Seem. The research method used a Randomized Complete Block Design (RCBD) that consist of three treatments (red light, far red light, dark) and one control (open air) with four replications for each group. Each group consist of 10 seeds. Precentage of seed germination; seedling height; cotyledon width; length, width and number of leaves were measured. The result showed that seed germination start at third day after sowing.  Germination rate and germination rate coefficcient showed there is no significant difference between all treatments even though far red light showed highest number (100 %; 0.755).  However, light color influence on seedling growth. Far red light showed affect on internodes distance. Dark light showed affect on leaves and cotyledon size. Red light and control showed similarity.</p><p> </p><div><p class="Els-keywords"><strong>Keywords:</strong> dark; far red light<em>, </em><em>Markhamia stipulata</em> (Wall.) Seem; red light; seed germination</p></div>


2021 ◽  
Vol 264 ◽  
pp. 04033
Author(s):  
Bakhadir Mirzaev ◽  
Farmon Mamatov ◽  
Bakhtiyor Tulaganov ◽  
Amir Sadirov ◽  
Rustam Khudayqulov ◽  
...  

One of the problems in the seed production of pasture forage plants is low seed germination. Existing pasture improvement technologies and technical means for seed production, harvesting, post-harvest handling, and storage of pasture forage plants are analysed. Suggestions for improving seed germination have been developed: it is advisable to place the seed crops between the forest belts; to improve seed germination, it is necessary to make a preliminary harvest of seed crops with spreading the mass on the stubble; the mechanised collection of seed heaps using a rotary mower with an active heap separator increases the germination rate by reducing mechanical damage to the seeds and enriching the soil with organic matter; the seed sowing process has a positive effect on the germination rate of the seed produced, in addition to reducing cleaning costs.


Author(s):  
T. Chitdeshwari ◽  
P.M. Brindhavani

Background: Soil calcareousness is the major problem in arid and semi arid regions globally since lime induced chlorosis lead to the deficiency of many essential plant nutrients which in turn resulted in reduced growth and yield loss of many crops. To explore the effects of various concentrations of calcium carbonate (CaCO3) on seed germination and seedling growth of groundnut genotypes and to identify CaCO3 stress tolerant and sensitive groundnut genotypes. Methods: The seed germination and seedling growth of ten groundnut genotypes (CO 6, CO 7, TMV 2, TMV 10, TMV 13, TMV 14, VRI 7, VRI 8, ALR 2 and BSR 2) was investigated with CaCO3 concentrations ranging from 1 to 100 mM L-1 for a period of 14 days. Result: The genotypes VRI 8 and TMV 10 showed higher seed germination and seedling growth even at higher CaCO3 concentration (upto 20 mM L-1) and considered as tolerant genotypes. Whereas TMV 2 and VRI 7 exhibited drastic reduction even at 1 mM L-1 and confirms their susceptibility to CaCO3 stress. Higher concentrations of CaCO3 in the growing medium greatly decreased the germination rate, germination index and vitality index but the impact differs with potentials of genotypes studied.


2018 ◽  
Vol 1 (1) ◽  
pp. 09-18
Author(s):  
Indra Dwipa ◽  
Muhsanati Muhsanati

Most of rice seeds experience after ripening problem which then result in seed dormancy. This kind of dormancy could be broken through various types of treatments, including storaging in dry place for certain period. This study was aimed to evaluate the combination between seed water content and storage duration to induce the seed germination of local genotype brown rice, Daro Merah. Various levels of seed water content (6-10, 11-15, 16-20 and 21-25 %) and storage duration (2, 4, 6 and 8 weeks) were evaluated to identify its effect on Daro Merah seed germination. Data were analyzed using two-way analysis of variance where significance were further proceeded using Duncan’s New Multiple Range Test with a p>0.05. The results showed that seed germination of Daro Merah reached 95 % at five days after seeding when the seed water content was maintained at 6-10% and stored for 8 weeks. After 14 days, the germination reached 99 % and most of the seeds showed some shoots emerged from the soil. Keywords: Brown rice, germination, seed water content, storage duration, Daro Merah


2017 ◽  
Vol 20 (1) ◽  
pp. 45-56
Author(s):  
P Saha ◽  
MSU Bhuiya ◽  
B Karmakar ◽  
M Salim ◽  
B Ahmed ◽  
...  

The experiment was conducted at the Agronomy research field of Bangladesh Agricultural University, Mymensingh from July to December 2013 to find out the effect of seedling age and storage duration on growth and yield of wet season rice. The experiment comprised of four ages of seedling (25, 30, 35 and 40-d-old) and four storage durations (0, 1, 2 and 3-days) of uprooted seedlings. Rice var. BRRI dhan52 was used as a test variety. The experiment was laid out in a split-plot design with 3 replications placing seedling age in the main plot and storage duration in the sub- plot. The effect of age and storage -duration of seedling, and their interaction were significant on growth parameters (shoot height, root length, number of tillers hill-1, leaf area index and crop growth rate), grain yield and straw yield. The growth parameters and yield exhibited a trend of decrease with the increase in seedling age and storage duration. All the growth parameters and yield showed highest value in the younger seedlings of 25-d-old with 0-day storage duration of uprooted seedlings while the lowest in the older seedlings of 40-d-old with 3-days of storage duration. The var.BRRI dhan52 produced the highest LAI (8.23), CGR (7.33 mg day-1 hill-1) and biomass (26.87 g) at 60, 45 and 60 days after transplanting. Grain yield reduced by 13, 19 and 37% of 30, 35 and 40-d-old seedlings, respectively compared to 25-d-old seedlings. In case of seedling storage, grain yield reduced by 5, 14 and 31% of 1, 2 and 3-d storage, respectively compared to 0-d storage. For optimum growth and yield of wet season rice, 25-d-old seedlings may be stored up to 1-day after uprooting taking into account the possible delay in transporting seedlings to the flood affected area from the nearby districts.Bangladesh Agron. J. 2017, 20(1): 45-56


2019 ◽  
Vol 46 (2) ◽  
pp. 148-161
Author(s):  
E.T. Carter ◽  
D.L. Rowland ◽  
B.L. Tillman ◽  
J.E. Erickson ◽  
T.L. Grey ◽  
...  

ABSTRACT Peanut is an important oilseed crop and legume species, with more than 1.9 M tons produced annually in the U.S. Being indeterminate, peanut continually flowers and sets pods throughout the growing season, leading to the potential harvest of both mature and immature pods. To quantify the physiological impacts of peanut seed maturity, a two-year field study was conducted to elucidate the difference in canopy structure and reproductive characteristics, including flower production, yield, and grade between seed obtained from immature and mature seed of two commercial peanut cultivars: TUFRunner™ ‘727’ and FloRun™ ‘107’. Data indicated that seed from the yellow class of pods have lower vigor and overall plant development and performance; further, plants developed from immature seed never achieved a level of performance comparable to that of the mature brown/black pod classes. There were differences between cultivars in the severity of the impact of immaturity, with larger detrimental effects on immature TUFRunner™ ‘727’, which exhibited reduced emergence. Despite these cultivar differences, this study illustrated that mature seed performs better in a field setting than immature seed. These results are critically important to disproving the ‘catch-up' assumption: seed maturity not only has an impact on emergence, but on subsequent life history and performance traits through the remainder of the season.


2011 ◽  
Vol 35 (6) ◽  
pp. 1141-1148 ◽  
Author(s):  
Rade Stanisavljevic ◽  
Dragoslav Ðjokic ◽  
Jasmina Milenkovic ◽  
Lana Ðukanovic ◽  
Vladeta Stevovic ◽  
...  

During post-harvest maturation, different species vary in the length of dormancy breaking or germination increases. Seed dormancy and slow seedling development often limit establishment of forage grass stands. Seed germination and seedling vigour of Italian ryegrass (Lolium italicum A. Braun, Synonym Lolium multiflorum L.), cocksfoot (Dactylis glomerata L.) and timothy (Phleum pretense L.) were observed after harvest and storage. After harvest in June, seeds were stored under standard storage conditions and sampled every 30 days after harvest (DAH), up to 270 DAH, and then every 60 days up to 990 DAH. At each date, seeds were tested for final germination percentage and for seedling vigour traits. Timothy seeds had a maximum germination (88%) and the best seedlings vigour at 90 DAH, which implies that early autumn (September-October) is the best sowing period for freshly harvested seeds of timothy. Timothy seed germination was poor from 270 DAH (73%). The best germination and vigour of Italian ryegrass and cocksfoot seedlings were between 270 and 330 DAH, which equates to spring sowing time (March-April) in the succeeding year. Cocksfoot and Italian ryegrass seeds maintained satisfactory germination levels up to 630 DAH (81%) and 810 DAH (81%), respectively. The data can serve for the determination of a proper storage duration management between harvest and sowing of the tested species under ambient conditions of south-eastern Europe.


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