Qualitative and quantitative differences in the isoelectrofocusing profile of biologically active lutropin in the blood of normally menstruating and post-menopausal women

1981 ◽  
Vol 97 (2) ◽  
pp. 166-175 ◽  
Author(s):  
F. Strollo ◽  
J. Harlin ◽  
H. Hernandez-Montes ◽  
D. M. Robertson ◽  
A. A. Zaidi ◽  
...  
Keyword(s):  
Molecular Species ◽  
Temporal Changes ◽  
Biologically Active ◽  
The Other ◽  
Plasma Samples ◽  
Menopausal Women ◽  
Menstruating Women ◽  
Post Menopausal ◽  
Ph Range ◽  
Alkaline Material

Abstract. A single bolus of 100 μg of gonadoliberin (LRH) was administered intravenously to 8 post-menopausal and 9 normally menstruating women and blood was withdrawn before and 30, 60, 120 and 240 min after LRH stimulation. The plasma samples obtained at different time intervals from women showing a sufficiently high response to LRH (menopausal: 8, menstruating: 3) were combined and 2 ml samples of each pool were fractionated in triplicate by electrofocusing on sucrose density gradient. In addition, two plasma pools, obtained 30 min following LRH stimulation, one from 4 normally menstruating women (exhibiting a relatively low LH-response) and the other from 2 normally menstruating women aged 40, were analyzed in the same way in duplicate electrofocusing experiments. The hLH activity was determined in each electrofocusing fraction by an in vitro bioassay method following elution and purification by gel filtration. The LH activity was distributed as four major peaks at pI values of 7.10 ± 0.05, 7.58 ± 0.06, 8.10 ± 0.04 and 8.54 ± 0.05 and a broad area of activity comprising a number of peaks in the pH range of 8.69–9.50. The analysis of the data revealed marked differences in the relative distribution of the various molecular species present in the blood of menopausal women and of normally menstruating women. A molecular species exhibiting a pI value of 7.10 was invariably present (10 – 15% of the total) in all samples of post-menopausal plasma (PMP) but was consistently absent from all samples of midcycle plasma (MCP). The amount of relatively 'less alkaline' material (eluted from pH range 7.37–8.32) was significantly higher (P < 0.001) in the PMP samples compared to MCP samples. On the other hand, in the MCP samples the amount of relatively 'more alkaline' material eluted from the pH range 8.33–9.50 was significantly (P < 0.001) higher (about 60% of the total recovered activity) compared to the PMP samples (about 30% of the total). Following LRH stimulation significant temporal changes were observed in the relative contribution of various molecular species to the hLH profile. A gradual increase, up to 60 min, in the material eluted in the pH range 6.87–7.36 in the post-menopausal plasma samples was accompanied by a gradual decrease in the material eluted in the pH range 7.84–8.32. Two hours after LRH stimulation a significant drop was found in the material collected from pH range 8.33–8.68, with a concomitant rise in the material eluted in the pH range 8.69–9.50. This last mentioned shift was also observed in the plasma of normally menstruating women. It is concluded that major differences exist in the composition of biologically active hLH species present in the peripheral blood of post-menopausal and normally menstruating women. Moreover, significant temporal changes occur in the composition of circulating hLH species following stimulation by LRH both in post-menopausal and in normally menstruating women.

SERUM THYROTROPHIN, PROLACTIN AND GROWTH HORMONE, RESPONSE TO TRH DURING OESTROGEN TREATMENT

1977 ◽  
Vol 84 (1) ◽  
pp. 23-35 ◽  
Author(s):  
E. Rutlin ◽  
E. Haug ◽  
P. A. Torjesen
Keyword(s):  
Growth Hormone ◽  
Human Subjects ◽  
Serum Levels ◽  
Normal Male ◽  
Oestrogen Treatment ◽  
Thyrotrophin Releasing Hormone ◽  
Basal Serum ◽  
Menopausal Women ◽  
Menstruating Women ◽  
Post Menopausal

ABSTRACT The serum levels of thyrotrophin (TSH), prolactin (PRL) and growth hormone (GH) and the response of these hormones to 500 μg thyrotrophin-releasing hormone (TRH) iv were studied in menstruating women. in post-menopausal women before and after 2 mg oestradiol valerate for 5 consecutive days, and in men on long term oestrogen treatment. Oestrogen treatment had no effect on basal serum TSH levels, which were within the normal range in all groups. The TSH response to TRH was not different in menstruating and post-menopausal women and was not changed in the latter group after oestrogen treatment. In men treated chronically with oestrogens, the TSH response to TRH was similar to that found in normal male subjects. There was no difference in basal levels of serum PRL between males and menstruating females. In the post-menopausal women, however, basal levels of serum PRL was significantly decreased, but rose during oestrogen treatment to serum levels normally found in menstruating women. In the oestrogen treated males basal serum PRL levels were significantly higher than in untreated men. The PRL response to TRH was significantly greater in females than in males, but in the oestrogen treated males the PRL response to TRH was greatly increased and almost of the same magnitude as the response in females. There was no difference in PRL response between menstruating and post-menopausal women, and oestrogen treatment of the latter group had no significant effect on the PRL response. Basal levels of serum GH did not differ between the groups. In the group of 9 post-menopausal women one subject showed a small GH response to TRH prior to oestrogen treatment, while 7 subjects showed GH responses to TRH after oestrogen treatment. In the group of 5 chronically oestrogen treated men, 2 subjects had increased serum levels of GH after TRH. Thus our data show that oestrogen administration may induce PRL release in human subjects, while oestrogens seem to play a far less important role in the regulation of GH and TSH secretion.

277. Equine chorionic gonadotrophin isoform composition in commercial products compared with isoform composition in pregnant mare plasma

2008 ◽  
Vol 20 (9) ◽  
pp. 77
Author(s):  
U. A. Ciller ◽  
I. M. Ciller ◽  
J. R. McFarlane
Keyword(s):  
Liquid Phase ◽  
Chorionic Gonadotrophin ◽  
The Other ◽  
Acidic Ph ◽  
Plasma Samples ◽  
Glycoprotein Hormone ◽  
Livestock Industry ◽  
Horse Plasma ◽  
Ph Range ◽  
High Degree

It is well documented that there is considerable batch to batch variation in the activity of commercial preparations of gonadotrophins. These products are used in a variety of assisted reproductive procedures in the livestock industry, consequently this high degree of variation between products and batches adds to the already considerable between animal variations in response to the treatment. Equine chorionic gonadotrophin (eCG) is a heterodimeric glycoprotein hormone secreted by the placental endometrial cups during the first third of gestation in the horse. Plasma is harvested from pregnant mares between 40–90 days of gestation and the eCG isolated and used to formulate commercial preparations. Previous research has shown that eCG like the other gonadotrophins is a highly heterogeneous molecule with significant differences in bioactivity between isoforms. The aim of this study was to determine whether significant differences in isoform composition exist between various commercial preparations of eCG (n = 15), and how this compares with the isoform composition found in plasma (n = 23). Concentrations of eCG were determined using a competitive eCG ELISA. Liquid phase iso-electric focusing was used to fractionate plasma and the commercial preparations into 10 pH ranges from pH 3.0 to pH 10.0. Data from the 10 fractions were grouped into acidic (pH 3.0–5.1), intermediate (pH 5.2–7.9), or basic (pH 8.0–10.0) isoform categories for analysis. Immunoactivity between commercial eCG products ranged from 44% to 362% of stated bioactivity. Iso-electric focusing showed that the majority of the immunoactivity (92%) of the commercial preparations was found in the acidic fractions (pH 3.0–5.1), and in particular in the pH range 3.0–3.8. This contrasted starkly with isoform profiles found in pregnant mare plasma samples which showed a much greater spread across all 3 pH ranges. In summary, the isolation processes of commercial eCG preparations appears to selectively favour the acidic isoforms of eCG.

STUDIES ON SALIVA IN MENSTRUATING, PREGNANT AND POST-MENOPAUSAL WOMEN

1965 ◽  
Vol 48 (2) ◽  
pp. 329-336 ◽  
Author(s):  
S. Kullander ◽  
B. Sonesson
Keyword(s):  
Pregnant Women ◽  
Water Content ◽  
Excretory Duct ◽  
Menopausal Women ◽  
Significant Difference ◽  
Menstruating Women ◽  
Two Phases ◽  
Post Menopausal ◽  
Spontaneous Secretion ◽  
Fertile Women

ABSTRACT Saliva from normally menstruating, pregnant and post-menopausal women was collected by catheterizing the excretory duct of the submandibular gland. The saliva was studied for rate of secretion, water content and the crystallization pattern. In addition the spontaneous secretion of saliva as well as the secretion stimulated by histamine and by pilocarpine were studied. The observations can be summarized as follows: Histamine had no effect on the rate of secretion, while pilocarpine increased the secretion considerably. In fertile women, the rate of spontaneous and histamine-stimulated secretion was somewhat higher during the secretion phase of the menstrual cycle than during the proliferation phase. No significant difference was found between the water content of the saliva in the two phases. In pregnant women there was a decrease in the rate of secretion as compared with normally menstruating women. In the post-menopausal women the decrease in secretion of saliva was still more marked. In pregnant women the crystallization pattern of the saliva was coarser than in menstruating women. After the menopause the crystallization pattern was still coarser and in one case no crystallization occurred at all. No constant differences were observed between the patterns during the proliferation and secretion phases, respectively.

Assessment of Nutritional Status of Menopausal Women

2016 ◽  
Vol 53 (2) ◽  
pp. 184
Author(s):  
Raut Sonali ◽  
Nalwade Vijaya
Keyword(s):  
Body Weight ◽  
Nutritional Status ◽  
Haemoglobin Level ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Mean Value ◽  
The Other ◽  
Menopausal Women ◽  
Post Menopausal

The present study was undertaken to assess the nutritional status of menopausal women. Anthropometric measurements and biochemical parameters such as serum calcium and haemoglobin level of blood was estimated. Results indicated that mean values of body weight, BMI and hip circumference were significantly more among post menopausal women than that of pre menopausal and peri menopausal women whereas height of peri menopausal women was significantly more than that of pre menopausal and post menopausal women. On the other hand, waist hip ratio was significantly more among pre menopausal women than the other two groups of menopausal women. Mean value of serum calcium level was more in pre menopausal than that of peri menopausal and post menopausal women but significant difference was noticed among only in serum calcium level of peri menopausal and post menopausal women. More per cent of the pre menopausal women were having normal serum calcium (mg/dl) level than that of peri menopausal women and post menopausal women. Mean haemoglobin level in the blood of the menopausal women in the three stages did not differ significantly (p&gt;0.05) and the mean value of haemoglobin of all the three groups of selected menopausal women was less than normal values. Whereas more per cent of pre menopausal women were found to be under normal haemoglobin category than that of peri menopausal and post menopausal women. On the whole the results indicated that women tend to gain body weight as they tend to reach towards the menopausal stage.

The Effects of Therapy with Oestriol Succinate and Ethinyl Oestradiol on the Haemostatic Mechanism in Post-Menopausal Women

1976 ◽  
Vol 35 (02) ◽  
pp. 403-414 ◽  
Author(s):  
Terence Davies ◽  
Gillian Fieldhouse ◽  
George P. McNicol
Keyword(s):  
Coagulation Factors ◽  
Factor Vii ◽  
Oestrogen Therapy ◽  
Qualitative And Quantitative ◽  
Lysis Activity ◽  
Menopausal Women ◽  
Increased Sensitivity ◽  
Post Menopausal ◽  
Incremental Increase ◽  
Ethinyl Oestradiol

SummaryThe effects on the haemostatic mechanism of oestrogen therapy, given to prevent bone loss in post-menopausal women, have been investigated. Oestriol succinate was given orally to 10 women at a level of 2 mg/day for 1 month and for a further 3 months with incremental increase of 2 mg each month. 6 of the 10 women were subsequently treated with 25 μg/day orally of ethinyl oestradiol. Oestriol succinate therapy resulted in a small increase in the level of factor VII, a decrease in factor VIII concentration and increased sensitivity of platelets to aggregating agents. Ethinyl oestradiol treatment resulted in much more widespread changes with marked increases in coagulation factors VII, VIII, IX and X, decreased levels of antithrombin and dramatic increases in circulating plasminogen levels and euglobulin lysis activity. The data suggested that the nature of oestrogens employed therapeutically is important in determining the qualitative and quantitative effect of oestrogen therapy on components of the haemostatic mechanism.

PLASMA CORTISOL, CORTICOSTERONE AND NON-PROTEIN-BOUND CORTISOL IN EXTRACORPOREAL CIRCULATION

1972 ◽  
Vol 69 (3) ◽  
pp. 517-525 ◽  
Author(s):  
T. Uozumi ◽  
H. Manabe ◽  
Y. Kawashima ◽  
Y. Hamanaka ◽  
Y. Monden ◽  
...  
Keyword(s):  
Cortisol Level ◽  
Extracorporeal Circulation ◽  
Plasma Cortisol ◽  
Elevated Level ◽  
Marked Decrease ◽  
Binding Capacity ◽  
Biologically Active ◽  
Major Surgery ◽  
Plasma Samples ◽  
Effective Factor

ABSTRACT The response of plasma cortisol, corticosterone and non-protein-bound cortisol in the extracorporeal circulation was investigated in 14 patients. The pre-perfusion levels of plasma cortisol, corticosterone and non-protein-bound cortisol were significantly elevated. During and immediately after perfusion, the levels of cortisol and corticosterone were found to decrease significantly from the pre-perfusion levels, while the percentage of non-protein-bound cortisol was shown to increase significantly. This indicates a marked decrease in cortisol binding capacity of plasma during extracorporeal circulation. Moreover in 200 plasma samples, it was demonstrated that the cortisol level increased markedly and the cortisol binding capacity decreased slightly during and shortly after major surgery without perfusion. It is concluded that stressful situations in major surgery with or without perfusion are associated with markedly increased levels of biologically active non-protein-bound cortisol. The elevated level of non-protein-bound cortisol in surgery seems to be dependent on the increase in the level of plasma cortisol as well as on the decrease in the cortisol binding capacity of plasma. Although the increased plasma cortisol plays the most important role in surgery with no perfusion, the decreased cortisol binding capacity may be the more effective factor involved during perfusion.

Effects of calcifediol versus cholecalciferol on 25(OH)D3 serum levels, appendicular muscle strength, and physical performance in post-menopausal women

2016 ◽  
Author(s):  
Antimo Moretti ◽  
Sire Alessandro de ◽  
Dario Calafiore ◽  
Raffaele Gimigliano ◽  
Francesca Gimigliano ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Physical Performance ◽  
Serum Levels ◽  
Menopausal Women ◽  
Post Menopausal
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