Hypertrophy and hyperplasia during goitre growth and involution in rats separate bioeffects of TSH and iodine

1987 ◽  
Vol 116 (4) ◽  
pp. 537-548 ◽  
Author(s):  
D. Stübner ◽  
R. Gärtner ◽  
W. Greil ◽  
K. Gropper ◽  
G. Brabant ◽  
...  
Keyword(s):  
Serum Level ◽  
Iodine Deficiency ◽  
Tap Water ◽  
Iodine Content ◽  
Total Dna ◽  
Thyroid Hyperplasia ◽  
Tsh Levels

Abstract. Goitre growth was investigated in rats receiving a low iodine diet (< 0.1 μg iodine/g) and either 1 g/l KClO4 or 1 g/l propylthiouracil (PTU), or a combination of KClO4 or PTU with 50.82 nmol/l T3 in tap water for 3 weeks. To investigate goitre involution, rats with iodine-deficient goitres were treated for 3 weeks either with T3 (0.5 μg T3/day = 0.768 nmol/day), iodide (0.5 or 2.7 μg KI/day) or a combination of T3 with both iodide doses. Histology together with total DNA distinguished between hypertrophy and hyperplasia of the gland. During goitre growth there was a highly significant correlation between goitre weight and TSH serum level (r = 0.93, P < 0.001). Thyroid total DNA, however, was only weakly correlated to TSH but was inversely related to the degree of iodine deficiency. During goitre regression, TSH levels were normalized, histological signs of hypertrophy had disappeared, and thyroid weight was nearly normalized in all therapy groups. Total DNA, however, was normalized only with 2.7 μg KI/day (95 ± 18 μg DNA/gland), and still elevated in all other groups. The highest DNA levels were found under T3 therapy (143 ± 21 μg DNA/gland) and under 0.5 μg KI/day (161 ± 19 μg DNA/gland). Reduction of total DNA was independent of TSH, but followed replenishment of the iodine content of the glands. We conclude that TSH mainly induces hypertrophy, whereas thyroid hyperplasia is mainly regulated by the intracellular iodine content.

RELATIONSHIP BETWEEN PEROXIDASE ACTIVITY AND SERUM TSH, T4 AND T3 LEVELS IN RATS IN THE COURSE OF IODINE DEFICIENCY

1978 ◽  
Vol 88 (3) ◽  
pp. 499-505 ◽  
Author(s):  
B. M. Nataf ◽  
P. Fragu ◽  
S. Ben Othman
Keyword(s):  
Iodine Deficiency ◽  
Peroxidase Activity ◽  
Control Diet ◽  
Early Period ◽  
Iodine Content ◽  
Thyroid Peroxidase ◽  
Wet Weight ◽  
Iodine Treatment ◽  
Serum Tsh ◽  
Tsh Levels

ABSTRACT The relationship between peroxidase activity and serum TSH, T4 and T3 levels was investigated in the course of iodine deficiency in rats. Rats were maintained either on a control diet with a relatively high iodine content (600 μg/kg of 127I), or on a low iodine diet (30 μg/kg of 127I). Twenty days after the low iodine treatment, the thyroid iodine [127I] concentration was half that of control value (647 ± 52 and 1241 ± 72 μg/g of wet weight, respectively). However, no significant changes in serum T4, T3, TSH were found at 20 days even though an early increase in peroxidase activity was observed. It was only at 35 days of iodine deficiency, when the concentration of iodine in the gland averaged 260 μg/g of wet weight that serum T4 and TSH levels started to be significantly modified. From day 35 to day 70, a significant and progressive decrease of plasma T4 concentration was observed, and it levelled off thereafter. The changes of serum T3 were much smaller than those of T4. A significant increase in serum TSH level was noted at 35 days. Thereafter TSH levels increased rapidly and progressively (205 % increase over control at 70 days and 643 % at 80 days). From day 35 until day 80 of the low iodine treatment, the thyroid peroxidase activity and the serum TSH level varied concomitantly. Our results suggest that for an iodine content between 5 and 2 μg per thyroid gland, the high cellular peroxidase activity observed could be correlated with an increase in circulating TSH, due to a decrease of T4. In contrast, in the early period of iodine deficiency, no correlation was found between peroxidase activity and serum T4, T3 and TSH levels.

INDUCTION OF GOITRE BY PTU OR KClO4 IN MALE AND FEMALE RATS. EFFECT OF GONADECTOMY

1973 ◽  
Vol 74 (1) ◽  
pp. 88-104 ◽  
Author(s):  
T. Jolín ◽  
M. J. Tarin ◽  
M. D. Garcia
Keyword(s):  
Iodine Content ◽  
High Plasma ◽  
Disc Electrophoresis ◽  
Female Rats ◽  
Male Rats ◽  
Adult Rats ◽  
Male And Female ◽  
Glucose Levels ◽  
Male And Female Rats ◽  
Tsh Levels

ABSTRACT Male and female rats of varying ages were placad on a low iodine diet (LID) plus KClO4 or 6-propyl-2-thiouracil (PTU) or on the same diet supplemented with I (control rats). Goitrogenesis was also induced with LID plus PTU in gonadectomized animals of both sexes. The weight of the control and goitrogen treated animals, and the weight and iodine content of their thyroids were determined, as well as the plasma PBI, TSH, insulin and glucose levels. The pituitary GH-like protein content was assessed by disc electrophoresis on polyacrylamide gels. If goitrogenesis was induced in young rats of both sexes starting with rats of the same age, body weight (B.W.) and pituitary growth hormone (GH) content, it was found that both the males and females developed goitres of the same size. On the contrary, when goitrogenesis was induced in adult animals, it was found that male rats, that had larger B.W. and pituitary GH content than age-paired females, developed larger goitres. However, both male and female rats were in a hypothyroid condition of comparable degree as judged by the thyroidal iodine content and the plasma PBI and TSH levels. When all the data on the PTU or KClO4-treated male and female rats of varying age and B.W. were considered together, it was observed that the weights of the thyroids increased proportionally to B.W. However, a difference in the slope of the regression of the thyroid weight over B.W. was found between male and female rats, due to the fact that adult male rats develop larger goitres than female animals. In addition, in the male rats treated with PTU, gonadectomy decreased the B.W., pituitary content of GH-like protein and, concomitantly, the size of the goitre decreased; an opposite effect was induced by ovariectomy on the female animals. However, when goitrogenesis was induced in weight-paired adult rats of both sexes, the male animals still developed larger goitres than the females. Among all the parameters studied here, the only ones which appeared to bear a consistent relationship with the size of the goitres in rats of different sexes, treated with a given goitrogen, were the rate of body growth and the amount of a pituitary GH-like protein found before the onset of the goitrogen treatment. Moreover, though the pituitary content of the GH-like protein decreased as a consequence of goitrogen treatment, it was still somewhat higher in male that in female animals. The present results suggest that GH may somehow be involved in the mechanism by which male and female rats on goitrogens develop goitres of different sizes, despite equally high plasma TSH levels.

scholarly journals People's Knowledge and Practice of Salt Iodization, as Well as the Assessment of Iodine Content in Salt in the Cumilla Region of Bangladesh

2021 ◽  
pp. 12-24
Author(s):  
Sadia Jahan ◽  
Md. Saddam Hossain ◽  
Md. Anisur Rahman Bhuiyan ◽  
Susmita Roy Lisa ◽  
Somaia Haque Chadni
Keyword(s):  
Iodine Deficiency ◽  
Rural Areas ◽  
Iodine Content ◽  
Iodine Concentration ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Salt Iodization ◽  
Knowledge And Practice ◽  
Urban And Rural Areas ◽  
And Behavior

Aim: To evaluate the household iodine content and knowledge, attitude, and behavior regarding salt iodization among the residents of Cumilla, Bangladesh. Subjects and Methods: We conducted a cross-sectional survey of 700 inhabitants in Cumilla's urban and rural areas to determine the iodine concentration of salt they consume. The participants were asked about what they know about iodine deficiency and salt iodization as well as how their salt was packaged and stored. Among them, 338 people provided a sample of salt to be tested of its iodine content by the titrimetric method. Results: 46.57% of people knew that iodization was the best way to prevent iodine deficiency while 35.14% considered salt iodization during purchasing. Most people stored salt in plastic boxes (89.7%) and closed containers (84.14%).Among all participants, only 37.14% of people were aware of the iodine requirement during pregnancy. In urban regions, the median iodine content was 36.76 ppm (OR=0.658, 95% CI, 0.469-0.925), while in rural areas, the median iodine content was 40.92 ppm (OR=1.188, 95% CI, 1.022-1.380). Iodine levels were less than 15 ppm (minimum limit) in 6.8% of samples and greater than 40 ppm (maximum limit) in 56.5%. Only 36.7% of the salt samples contained adequate iodine. Conclusion: An effective and consistent approach for controlling iodine concentration in distributed salt is required at Cumilla, Bangladesh.

scholarly journals Iodine Content of Salt Use after Years of Universal Iodization Policy and Knowledge on Iodized Salt among Households in the Sissala East Municipality in Upper West Region of Ghana

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Prince Kubi Appiah ◽  
Clement Tiimim Yanbom ◽  
Martin Amogre Ayanore ◽  
Alex Bapula
Keyword(s):  
Public Health ◽  
Iodine Deficiency ◽  
Educational Level ◽  
Great Influence ◽  
Iodine Content ◽  
The Body ◽  
Iodized Salt ◽  
Health Concern ◽  
Good Knowledge ◽  
Salt Iodization

Background. Iodine deficiency is a global public health concern as it leads to inadequate production of thyroid hormone in the body, causing too many destructive consequences on the roles and functions of different human organs and muscles including brain growth and can manifest into many damaging effects such as intestinal cerebral impedance, cancer of intestine, breast disorders, and physical deformities like goitre and cretinism to one’s body. Despite all these negative effects, there are several important public health programs including universal salt iodization (USI) to improve on households’ iodine intake, notwithstanding this, countless families are still eating foods containing less iodine or no iodine at all. Hence, this study examined the intake of iodized salt after years of universal salt iodization and the knowledge on iodized salt among households in the Sissala East Municipality. Method. A descriptive cross-sectional study was adopted to collect data for the study. Data were collected from women in charge of household meal preparation using a semistructured questionnaire and rapid field iodine test kits. The Statistical Package for Social Sciences (SPSS) version 20 was used for the data analysis and presented in tables and graphs. p value <0.05 was considered as statistically significant. Findings. Only 41.4% of the households have good knowledge on benefits of iodized salt and dangers associated with iodine deficiency. It was realized that the health workers (46.6%) and television were the main sources of information on iodized salt. Household salt usage with adequate (>15 ppm) levels of iodine was 44.0%; however 85.9% of the salts were stored in covered containers. The study showed significant associations between knowledge on iodized salt and educational level (p≤0.001), occupation (p=0.043), religion (p=0.027), and ethnic lineage (p=0.046). Also, the use of iodized salt showed associations with the educational level (p≤0.001), occupation (p=0.003), religion (p=0.042), and knowledge on iodized salt (p≤0.001). Conclusions. Only about 4 in 10 households were consuming salt with adequate iodine, and this coverage is very low compared with the 90% or more coverage recommended by WHO/UNICEF/ICCIDD. Having secondary and tertiary education and having good knowledge of iodized salt has a great influence on the use of iodized salt; however, with this low level of knowledge of importance of iodized salt among women responsible for house food preparations, there is the need for health professionals to intensify education and promotion on iodized salt in the area and to monitor and verify iodine content of salts produced and sold in the market all times, as the source of the salt might have contributed to the low levels of iodine in the household salt.

scholarly journals Moderate Iodine Deficiency Is Common in Pregnancy but Does Not Alter Maternal and Neonatal Thyroid Function Tests

2020 ◽  
Vol 11 ◽  
Author(s):  
Tal Schiller ◽  
Arnon Agmon ◽  
Viviana Ostrovsky ◽  
Gabi Shefer ◽  
Hilla Knobler ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Thyroid Function ◽  
Iodine Deficiency ◽  
Urinary Iodine ◽  
Iodine Content ◽  
First Trimester ◽  
Thyroid Function Tests ◽  
Neonatal Thyroid Function ◽  
Iodine Deficiency In Pregnancy ◽  
In Pregnancy

IntroductionAn Israeli national survey found that 85% of pregnant women had urinary iodine content (UIC) levels below the adequacy range (&lt;150 µg/L). Widespread desalinated water usage and no national fortification plan are possible causes. Studies assessing relationships between iodine status and maternal and neonatal thyroid function provided varying results. Our aims were to determine whether iodine deficiency was associated with altered maternal or neonatal thyroid function and the factors leading to iodine deficiency.MethodsA cross-sectional study including 100 healthy women without prior thyroid disease, in their first trimester of a singleton pregnancy were recruited from an HMO clinic in central Israel. The women were followed from their first trimester. All women completed a 24-h dietary recall and life habits questionnaires. We tested for UIC, maternal and neonatal thyroid function, maternal autoantibodies, and neonatal outcomes.ResultsMedian UIC in our cohort was 49 µg/L [25%–75% interquartile range (IQR) 16-91.5 µg/L], with 84% below adequacy range. No correlation was found between iodine deficiency and maternal or neonatal thyroid function which remained within normal ranges. Antibody status did not differ, but thyroglobulin levels were significantly higher in iodine insufficient subjects. UIC was higher in women consuming an iodine containing supplement. There was no association between UIC and dietary iodine content or water source.ConclusionsModerate iodine deficiency is common in our healthy pregnant women population. Our data imply that moderate iodine deficiency in pregnancy seem sufficient to maintain normal maternal and neonatal thyroid function.

scholarly journals Dramatic synergism between excess soybean intake and iodine deficiency on the development of rat thyroid hyperplasia

2000 ◽  
Vol 21 (4) ◽  
pp. 707-713 ◽  
Author(s):  
T. Ikeda ◽  
A. Nishikawa ◽  
T. Imazawa ◽  
S. Kimura ◽  
M. Hirose
Keyword(s):  
Iodine Deficiency ◽  
Rat Thyroid ◽  
Thyroid Hyperplasia

Effects of iodide and thyroxine on iodine-deficient mouse thyroid: a morphological and functional study

1986 ◽  
Vol 110 (2) ◽  
pp. 203-NP ◽  
Author(s):  
M.-C. Many ◽  
J.-F. Denef ◽  
S. Hamudi ◽  
C. Cornette ◽  
S. Haumont ◽  
...  
Keyword(s):  
Iodine Content ◽  
Serum Levels ◽  
Functional Study ◽  
Deficient Mouse ◽  
Cell Necrosis ◽  
Follicular Cells ◽  
Normal Appearance ◽  
Control Value ◽  
Thyroid Hyperplasia ◽  
Mouse Thyroid

ABSTRACT The effects of iodide and thyroxine (T4) on female mice fed a low iodine diet (LID) for 8 weeks were analysed by morphological, stereological and biochemical methods. Iodide was given at a dose of 10 μg/day (HID) or 1 μg/day (MID), either alone or together with daily injections of 1 μg T4 for 8 or 40 days. With HID, the thyroid weight and the numbers of follicles and cells remained higher than in controls, although cell necrosis occurred. Colloid volume increased and iodine was stored within the gland: a colloid goitre with non-functioning follicles was produced. With MID, the glands resumed an almost normal appearance. With T4 and LID, progressive normalization occurred, but after 40 days thyroid weight and numbers of follicles and cells remained higher than in controls. Glandular iodine content slowly increased and reached control value. The proportions of 125I-labelled tri-iodothyronine (T3) and T4 in thyroglobulin were reduced. With T4 and HID, the glands resumed a normal appearance. Neither necrosis nor folliculoneogenesis was noted. The proportions of 125I-labelled T3 and T4 in thyroglobulin were reduced, but T3 and T4 serum levels were higher than with HID. With T4 and MID, a normal state was obtained as early as day 8. After 40 days the gland was morphologically and functionally inactive. In conclusion, the association of T4 and iodide seems to be the best way to obtain a rapid and complete involution of thyroid hyperplasia. The administration of T4 prevents the deleterious effects of an excess of iodine on follicular cells, and causes the gland to enter a slow-functioning state. J. Endocr. (1986) 110, 203–210

scholarly journals Assessment of iodine deficiency disorders among population of district Chamba, Himachal Pradesh

2020 ◽  
Vol 7 (2) ◽  
pp. 542
Author(s):  
Sumita Kumari Sandhu ◽  
Arvind Kumar Dhiman
Keyword(s):  
Iodine Deficiency ◽  
Iodine Content ◽  
Himachal Pradesh ◽  
Iodine Deficiency Disorders ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Iodised Salt ◽  
Cooking Practices ◽  
Significant Public Health ◽  
In The Beginning

Background: Iodine insufficiency is prevalent throughout the world, and is rated as one of the most significant public health concerns in more than 125 countries. Secondary to the insufficient dietary consumption of iodine, almost two billion individuals are prone to suffer from iodine deficiency disorders worldwide. The present survey was conducted in district Chamba of Himachal Pradesh in order to assess the extent of iodine deficiency disorders, 55 years after the initiation of salt iodisation programme.Methods: A cross sectional survey was conducted in district Chamba of Himachal Pradesh. A close ended pretested questionnaire was used to collect the socio-demographic information of the participants. Iodine content of salt and water was assessed with the use of I-Check and AQUA test kits in the households.Results: A total of 230 households were surveyed for the consumption of iodised salt which showed that 100% of the study population was using packet iodised salt for consumption. Majority of the population (92.6%) were placing their salt container near chulah in their kitchen and 73.5% were adding the salt in the beginning of the cooking followed by 16.1% in the middle of the cooking. Iodine content of salt sample collected revealed that 95.7% contain only 7 ppm iodine and 99.6% of the water sample contains less than 0.1 level iodine in water testing.Conclusions: The results of study indicated that population of district Chamba is using iodised salt but there is still need of strengthening of monitoring system as well as health education regarding the cooking practices in the households.

scholarly journals Iodine Content of Packaged Salt and Related Knowledge and Storage Practices at Household Level in Dhaka City, Bangladesh

2020 ◽  
pp. 52-58
Author(s):  
Md Sujan Hossen ◽  
Md Nazrul Islam Khan
Keyword(s):  
Iodine Deficiency ◽  
Iodine Content ◽  
Cross Sectional Study ◽  
Iodized Salt ◽  
Dhaka City ◽  
Iodine Deficiency Disorders ◽  
Sample Collection ◽  
Cross Sectional ◽  
Household Level ◽  
And Storage

Aims: Iodine deficiency disorders are one of the major public health concerns in Bangladesh. Regular consumption of iodized salt can help combat these disorders. The aims of this study were to determine the content of iodine in edible packaged salt and to assess iodized salt related knowledge and storage practices in Dhaka City, Bangladesh. Study Design: The study was an experimental cross-sectional study. Place and Duration of Study: The present study was conducted from June 2019 to July 2019 in Dhaka City, Bangladesh. A total of 120 households were selected for interview and packaged salt sample collection. The chemical analysis was done in the Food Analysis Laboratory of Institute of Nutrition and Food Science, University of Dhaka. Methodology: A closed-ended questionnaire was used for collection of information. Iodometric titration method was used to determine the content of iodine in packaged salt samples. Results: The mean (±SD) iodine content in the salt samples was 31.469 (±10.196) ppm. More than 90% salt samples were adequately iodized. Twenty five percent of the respondents know that consumption of iodized salt helps prevent goiter. Only 10.8% of the respondents know that iodine content decreases if iodized salt is stored close to fire. About 87% of them store salt away from fire. Conclusion: Along with consuming packaged iodized salt, householders should be educated about iodized salt related knowledge and storage practices to control iodine deficiency disorders.

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