scholarly journals Meiotic epigenetic factor PRDM9 impacts sperm quality of hybrid mice

Reproduction ◽  
2020 ◽  
Vol 160 (1) ◽  
pp. 53-64
Author(s):  
Fitore Kusari ◽  
Ondrej Mihola ◽  
John C Schimenti ◽  
Zdenek Trachtulec
Keyword(s):  
Sperm Quality ◽  
Sperm Count ◽  
Hybrid Sterility ◽  
Genomic Analysis ◽  
Testicular Atrophy ◽  
Gene Encoding ◽  
Meiotic Progression ◽  
Hybrid Mice ◽  
Transgenic Male

Reduced fertility of male mouse hybrids relative to their parents, or hybrid sterility, is governed by the hybrid sterility 1 (Hst1) locus. Rescue experiments with transgenes carrying sequences within or near Hst1 manifested that Hst1 contains the gene encoding meiosis-specific histone methyltransferase PRDM9. The Prdm9 gene is responsible for partial meiotic arrest, testicular atrophy, and low sperm count in (C57BL/6J x PWD)F1 mouse hybrids. Here we report that these male hybrids suffer an additional reproductive disadvantage, decreased sperm quality, which is (i) further exacerbated by the introduction of long transgenes carrying sequences from Hst1 with incomplete Prdm9 into their genome and (ii) controlled by the Prdm9 dosage. These transgenic male hybrids displayed the features of severe oligoasthenoteratozoospermia (OAT), a human infertility syndrome characterized by a low number of spermatozoa with poor motility and morphological abnormalities. Analysis of spermiogenesis in these mice revealed acrosome detachment, aberrant elongation and condensation of the nucleus. As a result, the transgenic sperm had acrosome malformations, abnormal chromatin packaging, and fragmented DNA with elevated base oxidation, revealed by using multiple methods. Heterozygosity for one null Prdm9 allele improved meiotic progression and sperm quality of both non- and transgenic hybrids. Our results indicate that genomic analysis of OAT patients should include consideration of allelic variants in PRDM9, and our transgenic models can serve as tools to understand the diverse molecular processes that, when perturbed, can cause this disease.

Effect of new dietary supplement on sperm quality

2012 ◽  
Vol 153 (45) ◽  
pp. 1787-1792 ◽  
Author(s):  
Mária Horváth ◽  
Endre Czeizel
Keyword(s):  
Adverse Effect ◽  
Clinical Trial ◽  
Dietary Supplement ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Before And After ◽  
The Usa ◽  
New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

The Potential Effect of Mucuna pruriens Seed Extract on Sperm Quality experimental study on mice exposed to cigarette smoke

2021 ◽  
Vol 20 (4) ◽  
pp. 768-773
Author(s):  
Meidona Nurul Milla ◽  
Yani Istadi ◽  
Vania Shaula ◽  
Deastri Anjeas Wari ◽  
Chntyia Dwi Cahyani Puspitasari ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cigarette Smoke ◽  
Sperm Quality ◽  
Medical Science ◽  
Seed Extract ◽  
Reproductive Age ◽  
Testicular Atrophy ◽  
Mucuna Pruriens ◽  
Control Group

Background: Infertility has been more common problems among couple of reproductive age. One of the factors causing this disorder is unhealthy environmental factors including exposure to cigarette smoke. Polynuclear aromatic hydrocarbons (PAHs) in cigarette smoke can cause testicular atrophy, while the free radicals can inhibit the stages of spermatogenesis, and nicotine in cigarettes affects the brain dopamine levels affecting the levels of GnRH, and subsequently affect the levels of FSH and LH needed in spermatogenesis. The use of Mucuna pruriens seed extract containing antioxidants and L-dopa is expected to improve the quality of sperm after exposure to cigarette smoke. Objective: This study aimed to determine the effect of Mucuna pruriens seed extract on the sperm quality in mice exposed to cigarette smoke. Methods: This study was an experimental study with a post test only control group design. A total of 20 mice were divided into 4 groups of five mice each. All groups were exposed to cigarette smoke. Group 1 was the negative control exposed to cigarette smoke. Groups 2, 3, 4 were exposed to cigarettes smoke and given Mucuna pruriens seed extracts at the dose of 250; 300; and 350 mg/Kg BW/day. Parameters of sperm quality included concentration, morphology, motility and viability. Results: Post hoc tests showed there were significant differences among treatment groups. Conclusion: the administration of Mucuna pruriens seed extract affects the sperm quality of BALB/c mice exposed to cigarettes smoke. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.768-773

Evaluation of milt quality of Clarias anguillaris (Linnaeus, 1758) Broodstock fed varying inclusion levels of wild hibiscus Azanza garckeana pulp meal

2020 ◽  
Vol 34 (1) ◽  
pp. 23-31
Author(s):  
I.J. Ochokwu ◽  
M.O. Nwabunike ◽  
G.N. Udeh
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Control Diet ◽  
Feeding Trial ◽  
Inclusion Level ◽  
Fish Tank

Feeding trial was carried out to evaluate sperm quality of Clarias anguillaris broodstock fed varying inclusion levels of goron tula, Azanza garckeana pulp meal. The fish (mean weight 500g) were randomly distributed in concrete tanks (2 x 2 x 1.2m) at nine fish/tank in triplicates. Five isonitrogenous diets (40% CP) were formulated with the pulp meal incorporated at the rate of 0, 5, 10, 15, and 20% of the diet. Fish were fed at 3% of body weight for 56days. At the end of the feeding trial, results showed that dietary A. garckeana significantly (p<0.05) improved the milt quality of C. anguillaris. The broodfish fed 20% A. garckeana inclusion level had the highest (p<0.05) milt volume, sperm motility duration, testes lobe lengths, and whole sperm count while the least values of these variables were observed in group fed with the control diet (0% inclusion level). The findings conclusively revealed that increase in the level of A. garckeana pulp meal in the diet resulted into increase in sperm quality of C. anguillaris. Thus, the pulp meal can be freely utilized in catfish production in terms of sperm quality for mass fingerling production. Keywords: A. garckeana, Clarias anguillaris, sperm count, motility duration, milt volume.

scholarly journals HUMAN SPERM COUNT

2015 ◽  
Vol 22 (01) ◽  
pp. 013-017
Author(s):  
Jahangir Sultan ◽  
Kokab Kokab ◽  
Muneer Ahmed
Keyword(s):  
Environmental Factors ◽  
Human Activity ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Human Sperm ◽  
Factors Affecting ◽  
Parental Exposure ◽  
The World

Objectives: Reports from many parts of the world suggest that chemical andphysical agents in the environment, introduced and spread by human activity may affect fertilityin men. The objective of this article is to highlight the environmental factors and their associationto male sperm quality and count as well. Materials and Methods: This study focusing onexposure to environmental factors affecting the semen quality of the workers working in differentfactories for a period of 5 to 7 years in Lahore. Results: The results from this study suggest thatthere are many environmental factors which may affect semen quality and sperm count. It isnecessary to prevent parental exposure to the agents associated with those hazards.

scholarly journals Sperm Quality and Oxidative-Antioxidant Profile in Men Living in Different Regions of Siberia

2020 ◽  
Vol 10 (4) ◽  
pp. 369-372
Author(s):  
Nadezhda Kurashova ◽  
Bair Dashiev ◽  
Lyubov Kolesnikova
Keyword(s):  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Reproductive Age ◽  
Anthropogenic Pressure ◽  
Factors Affecting ◽  
Antioxidant Profile ◽  
Caucasian Race ◽  
Geographical Position

Background: The aim of this study was to assess the sperm quality and oxidative-antioxidant profile in men living in different regions of Siberia Materials and Methods: The study involved 125 men of reproductive age: 40 men (mean age of 24.8 years) living in Ulan-Ude, 35 men (mean age of 24.1 years) in Irkutsk, and 50 men (mean age of 24.6 years) in Novosibirsk. All men belonged to the Caucasian race and were physically healthy. Methods of standard clinical examination of fertile and infertile men included: an ultrasonic scan of scrotum and prostate, macroscopic and microscopic examination of ejaculate, and biochemical analysis. The semen analysis was performed in accordance with the WHO recommendations (2010). The study of sperm quality included measuring the volume and pH of the ejaculate, the concentration of spermatozoa, the proportion of motile sperm of categories A and B, and lipid peroxidation-antioxidant activity products. Of the participants in the study, the group of somatically healthy men living in Irkutsk had the highest sperm count (mln per ml): 1.5 times more than men in Novosibirsk and 1.3 times more than men in Ulan-Ude. At the same time, in the group of men in Irkutsk, a significant increase in the concentration of α-tocopherol was revealed: 1.6 times more than the men in Ulan-Ude and 1.8 times more than the men in Novosibirsk. Conclusion: The conducted studies of the quality of ejaculate and the characteristics of LPO processes in men from different cities of Siberia show that place of residence and ecological-geographical position are not the main reasons determining reproductive dysfunction, but can be important factors affecting the functioning of the reproductive system and determining the heterogeneity of male infertility in conditions of anthropogenic pressure.

scholarly journals Influence of environmental factors on the qualitative and quantitative parameters of male ejaculate (literature review)

2019 ◽  
Vol 23 (3) ◽  
pp. 530-534
Author(s):  
S.V. Khmil ◽  
O.Yu. Mayorova ◽  
I.V. Dudchuk
Keyword(s):  
Environmental Factors ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Toxic Substances ◽  
Normal Morphology ◽  
Qualitative And Quantitative ◽  
Literary Sources ◽  
The Impact

Today it is important to study the problem of male infertility, as well as to identify the factors of its occurrence. The purpose of the work is to evaluate the overall picture of the quality of ejaculate during the last 50 years and to determine the reasons for such changes. More than 40 literary sources were published for thorough analysis in the period 1984–2018 (the share of new references is 20%). There was a decrease in sperm concentration by 1.5% / year, total sperm count — 1.6% / year, total sperm motility — 0.4% / year, decrease in the percentage of progressive motility sperm by 5.5% / year and with normal morphology 2.2% / year. One of the main reasons for the deterioration of sperm quality is the impact of negative environmental factors — chemical and radioactive contamination, as well as electromagnetic radiation. Among the most toxic substances are the heavy metals, pesticides, bisphenol A, phthalates, polychlorinated biphenyls, dioxin and its derivatives. Thus, over the last 50 years there has been a decline in the qualitative and quantitative indices of ejaculate men worldwide. One of the reasons is the worsening environmental situation.

Semen Analysis Profile on Varicocele Patients at Dr. Ramelan Navy Hospital Surabaya during Period 2015

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
TIFFANY WONGSODIHARJO
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Secondary Data ◽  
Married Couples ◽  
Sperm Cells ◽  
Descriptive Research ◽  
Normal Limits

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>: Infertility has affected about 10–15 percent married couples. Almost half of infertility cases have been attributed to men with low sperm motility (asthenozoospermia), low sperm count (oligozoospermia) and abnormal sperm morphology (teratozoospermia). Sperm motility is one of the major determinants of male fertility and is required for successful fertilization. Semen analysis is a method to know the quality of semen. Semen analysis consists of macroscopic and microscopic examination of sperm cells, which are concentration, motility and morphology of sperm cells. </p><p><strong>Objective</strong>:<strong> </strong>This study aimed to obtain the semen analysis profile on varicocele patients at DR. Ramelan Navy Hospital Surabaya during period 2015.</p><strong>Method</strong>: This study used descriptive research design and quantitative method, by using the secondary data were collected from medical records during January – December 2015. <strong>Result</strong>: In this study the semen analysis of 36 patients with varicocele, 3 patients (8,33%) with oligozoospermia, 10 patients (27,78%) with asthenozoospermia, 20 patients (55,55%) with oligoasthenozoospermia, 3 patients (8,33%) with extreme oligoasthenozoospermia. <strong>Conclusion</strong>: This study has concluded that all varicocele patients have abnormalities in semen analysis (none of the sperm quality results within normal limits).

Dietary Supplementation of Antioxidants Improves Semen Quality of IVF Patients in Terms of Motility, Sperm Count, and Nuclear Vacuolization

2012 ◽  
Vol 82 (6) ◽  
pp. 391-398 ◽  
Author(s):  
Barbara Wirleitner ◽  
Pierre Vanderzwalmen ◽  
Astrid Stecher ◽  
Dietmar Spitzer ◽  
Maximilian Schuff ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Parameters ◽  
Class I ◽  
Antioxidative Therapy

Background: This study aimed to investigate the influence of an oral antioxidative supplementation on sperm quality of in vitro fertilization (IVF) patients, as analyzed by sperm motility according to the WHO criteria and motile sperm organelle morphology examination (MSOME). Methods: Semen samples were collected from 147 patients before undergoing an IVF/intracytoplasmic morphologically-selected sperm injection (IMSI) cycle and 2 - 12 months after an antioxidative supplementation. Semen analysis was evaluated according to WHO and MSOME criteria. Spermatozoa were grouped according to the size of nuclear vacuoles within the sperm’s heads. Patients were divided into oligoasthenoteratozoospermic (OAT) and non-OAT men. Between first and second semen analysis, patients were supplemented orally with an antioxidative preparation. Results: After the antioxidative therapy we observed a significant reduction in the percentage of immotile sperm cells in the patients. Additionally, the percentage of class I spermatozoa according to MSOME criteria was significantly higher after antioxidative supplementation. In OAT patients the percentage of class I sperm was found to be increased, although not significantly. However, we observed a drastic improvement in sperm motility as well as in total sperm count in this group. Conclusion: The results demonstrated a considerable improvement in semen quality, notably in OAT patients. Considering the putative relationship between semen quality on the one hand and reactive oxygen species on the other, the observed changes in the sperm parameters indicate that a decline in semen quality, and even subtle morphological changes, might be associated with oxidative stress. Our findings suggest that an antioxidative and micronutrient supplementation has a remarkable benefit for IVF patients having restricted sperm parameters, in particular.

scholarly journals Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin

2015 ◽  
Vol 14 (2) ◽  
pp. 98
Author(s):  
Fahmi Akbar ◽  
Agus Oman Sudrajat ◽  
Siti Subaidah
Keyword(s):  
Litopenaeus Vannamei ◽  
Sperm Quality ◽  
Sperm Count ◽  
White Shrimp ◽  
Hatching Rate ◽  
Eyestalk Ablation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Abnormal Sperm ◽  
Serum Gonadotropin

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could  influence  the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp <em>Litopenaeus vannamei</em> sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose  of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of <em>L. vannamei</em> shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keyword: PMSG, AD, sperm quality, <em>Litopenaeus vannamei</em></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan <em>pregnant mare serum gonadotropin</em> (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname <em>Litopenaeus vannamei</em>. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang <em>L. vannamei</em>. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: PMSG, AD, kualitas sperma, <em>Litopenaeus vannamei</em></p><p> </p>

EFFICIENCY OF USING GENODIAGNOSTIC TEST IN ASSESSMENT OF PERINATAL INFECTION OF YOUNG ANIMALS IN PREVENTION OF LEUKEMIA OF CATTLE

2020 ◽  
Vol 1 (4) ◽  
pp. 450-455
Author(s):  
N.G. Kozyreva ◽  
◽  
I.Yu. Abashin ◽  
L.A. Ivanova ◽  
◽  
...  
Keyword(s):  
Dairy Products ◽  
Preventive Measures ◽  
Leukemia Virus ◽  
Genomic Analysis ◽  
Positive Trend ◽  
Infection Frequency ◽  
Perinatal Infection ◽  
Serological Method ◽  
Drinking Milk

This article presents the results of determining the level of perinatal infection of calves with the bovine leukemia virus at the age from birth to colostrum intake and 1-1.5 months after birth when drinking milk from healthy individuals, excluding the alimentary transmission route, with an infection frequency of 5.4% in the surveyed farm. Improvement of molecular diagnostics based on genomic analysis in the scheme of complex antiepizootic/recreational measures for bovine leukemia has been carried out in order to improve the quality of dairy products. As part of the implementation and improvement of the scheme of preventive measures, a positive trend was found, expressed in a tendency to a 3.6-fold decrease in the frequency of detection of BLV infection of young animals. In the course of work, the efficiency of the use of a gene diagnostic test - multiplex PCR-RT – as a tool for the specific prevention of bovine leukemia of calves in the early stages of the disease was shown: the relative diagnostic sensitivity of the PCR-RT technique is at a level not lower than that of the serological method and differs in the side of overestimation by 3.8 times.

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