scholarly journals ID: 1088 Experimental culturing of chick embryo in shell-less culture system – the first research in Vietnam

2017 ◽  
Vol 4 (S) ◽  
pp. 176
Author(s):  
Quy Xuan Nguyen ◽  
Long Thanh Dang
Keyword(s):  
Chick Embryo ◽  
Traditional Method ◽  
Culture System ◽  
Lethal Effect ◽  
Calcium Lactate ◽  
High Rate ◽  
Chick Embryos ◽  
Ideal Model ◽  
Significant Difference ◽  
First Time

Chick embryo is an ideal model with numerous applications in biomedical research. Among a variety of methods have been carried out for culturing chick embryos, shell-less culture system has a large number of advantages on accessibility, observation and manipulation. In this study, chick embryos were transferred to the shell-less culture system and the development of the chick embryos were assessed. Correlation between the diameter of sinus terminalis on the surface of yolk sac and viability of the embryos would be evaluated. In addition, calcium lactate was added to the culture system in order to find out the optimal amount. After the experiments, results showed that there was no difference between embryos in shell-less culture system and traditional method during incubation period. Secondly, the proportion of live chick embryos until embryonic day 17 reached the highest rate at 87,5% when the diameter of sinus terminalis was between 16 and 21 mm. At last, there was no significant difference between the group with 250 mg calcium lactate supplemented as compared to no supplemented group. Calcium lactate had a lethal effect on chick embryos when the supplemented content was 550 mg. In conclusion, the shell-less culture system could be able to allow the survival of chick embryos until day 21, with high rate in day 17. Besides, this has been the very first time the shell-less culture system was performed in Vietnam.

scholarly journals Identification of increased amounts of UDP-glucuronyltransferase protein in phenobarbital-treated chick-embryo liver cells

1983 ◽  
Vol 214 (2) ◽  
pp. 517-523 ◽  
Author(s):  
B Burchell ◽  
G J Pratt ◽  
I Duffy ◽  
L West
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Microsomal Fraction ◽  
Microsomal Protein ◽  
Liver Cells ◽  
Transferase Activity ◽  
Chick Embryos ◽  
Embryo Liver ◽  
Liver Microsomal Fraction ◽  
First Time

UDP-glucuronyltransferase activity of neonatal-chick liver or phenobarbital-treated chick-embryo liver catalysed the glucuronidation of 1-naphthol, 4-nitrophenol and 2-aminophenol. Only low transferase activity towards testosterone was detected, and activity towards bilirubin was not detectable. Liver microsomal transferase activity towards the three phenols was increased approx. 20-50-fold by phenobarbital treatment of chick embryos or by transfer of liver cells into tissue culture. A single form of UDP-glucuronyltransferase, which appears to catalyse the glucuronidation of these three phenols, was purified to near homogeneity from phenobarbital-treated chick-embryo liver microsomal fraction for the first time. The use of this purified enzyme as a standard protein facilitated the identification of this protein in chick-embryo liver microsomal fraction. Further, the accumulation of this microsomal protein was observed following phenobarbital treatment of chick embryos and during tissue culture of chick-embryo liver cells. The value of this model system for the study of the induction of UDP-glucuronyltransferase by drugs and hormones is discussed.

scholarly journals CONVENTIONAL AND MOLECULAR STUDY OF Babesia spp. OF NATURAL INFECTION IN DRAGGING HORSES AT SOME AREAS OF BAGHDAD CITY, IRAQ

2019 ◽  
Vol 50 (3) ◽  
Author(s):  
Faraj & et al.
Keyword(s):  
Natural Infection ◽  
Age Groups ◽  
Molecular Study ◽  
High Rate ◽  
Theileria Equi ◽  
Phylogenic Tree ◽  
Significant Difference ◽  
Pcr Products ◽  
Pcr Method ◽  
First Time

 The present study was planned to investigate equine babesiosis in dragging horses in Baghdad city, Iraq by using microscopical and molecular (PCR) techniques. 150 blood samples of horses examined for Theileria equi and Babesia caballi. 16.66% (25/150) were positive by microscopic examination. No significant difference was observed in infection rates between male and female horses and among different age groups. The result showed that PCR method has high rate of infection36% (9/25). Nine positive PCR products were sequenced and deposited in Genebank data base for first time in Iraq, phylogenic analysis demonstrated that 5 sequences belongs to T. equi (MK350319, MK346272, MK346273, MK346274 and MK36275), while 4 sequence (MK346276, MK346277, MK346278 and MK350318) belongs to B. caballi, and mounted a low genetic variation 0.035 and 0.05 respectively, among other comparison isolates. In conclusion PCR technique followed by phylogenic tree analysis a reliable methods for epidemiological, diagnosis and identification of genetic variants studies.

Effect of weak electromagnetic field on cardiac work, concentration of thyroid hormones and blood aminotransferase level in the chick embryo

2013 ◽  
Vol 61 (3) ◽  
pp. 383-392 ◽  
Author(s):  
Krzysztof Pawlak ◽  
Andrzej Sechman ◽  
Zenon Nieckarz ◽  
Dorota Wojtysiak
Keyword(s):  
Heart Rate ◽  
Electromagnetic Field ◽  
Chick Embryo ◽  
Thyroid Hormones ◽  
Final Stage ◽  
Physiological Function ◽  
Heart Weight ◽  
Chick Embryos ◽  
Cardiac Work ◽  
First Time

The aim of the study was to determine the effect of alternating electromagnetic field (EMF; 50 Hz frequency, 50 and 100 μT induction) on cardiac work of the chick embryo. Eggs from the experimental groups were exposed to EMF throughout incubation. During the experiment, heart rate (ballistocardiographic method), thyroxine (T4) and triiodothyronine (T3) concentrations, heart weight, ventricle wall thickness, and levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. The results show, for the first time, that the exposure of chick embryos to EMF augments the heart rate, especially from 17 days of incubation. The increased heart rate in the embryos exposed to EMF was associated with considerable increases in plasma T4and T3concentrations, which were recorded during the final stage of embryogenesis. The significant effect of the 100-μT field on heart weight and blood AST levels in the embryos suggests that EMF has a direct effect on the physiological function of cardiac muscle.

scholarly journals Dependency of the Shell-Less Culture on Temperature and Rotating Velocity

2018 ◽  
Author(s):  
Massimo Fioranelli ◽  
Alireza Sepehri ◽  
Maria Grazia Roccia
Keyword(s):  
Chick Embryo ◽  
Culture System ◽  
Chick Embryos ◽  
Rate Of Growth

Recently, some authors have developed a shell-less culture system for chick embryos by using aplastic lm as culture vessels. We will show that velocity of rotation of vessels in incubator andtemperature have direct relations with growth of chick embryos. We also obtain a mathematicalrelation between velocity, temperature and rate of growth of chick embryo.

Clinical evaluation of a new thyroglobulin immunoradiometric assay in the follow-up of differentiated thyroid carcinoma

2003 ◽  
Vol 42 (02) ◽  
pp. 71-77 ◽  
Author(s):  
I. Schreivogel ◽  
C. Angerstein ◽  
U. Siefker ◽  
K. Lehmann ◽  
G. Altenvoerde ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Residual Disease ◽  
False Negative ◽  
Differentiated Thyroid Carcinoma ◽  
Threshold Concentration ◽  
High Rate ◽  
Functional Assay ◽  
Hypothyroid Patient ◽  
Assay Sensitivity ◽  
Significant Difference

SummaryAim: Formal and clinical comparison of a new 3rd-gene-ration-Tg-IRMA (3-G-IRMA; Dynotest®Tg-plus) with a conventional Tg-IRMA (3-G-IRMA; SELco®Tg-assay) for patients with differentiated thyroid carcinoma. In addition we evaluated, if thyroglobulin (Tg) levels above a specific threshold concentration indicate the need for further investigations for residual disease. Patients, methods: Tg concentration of 105 sera of 93 consecutive patients with a differentiated thyroid cancer was determined with both assays and compared at different cut-off values (Dynotest®Tg-plus: 0.2, 1, 2 ng/ml; SELco®Tg-assay: 0.5, 1, 2 ng/ml) with the clinical results in respect to the corresponding TSH concentration. Results: Tg concentration did not show any significant difference (SELco®Tg-assay 0.5 ng/ml, Dynotest® Tg-plus 0.2 ng/ml). The Tg-values of both assays correlated with 97%. However, correlation of recovery in both assays was small (40%). The sensitivities and specificities of both assays at different cut-offs and TSH values did not reveal significant differences. In patients with TSH concentration >30 µU/ml the functional assay sensitivity was superior to arbitrary cut-offs in the decision to start further evaluations. Conclusions: In our study neither formal nor clinical significant differences between two Tg-assays were found. In a hypothyroid patient (TSH >30 µU/ml, Tg concentration exceeding the functional assay sensitivity) further investigations for residual disease are warranted. Higher thresholds are of limited value, due to a inacceptable high rate of false negative results.

Competition of Verb Aspects and Its Resolution as a Creative Act (Based on the Material of Aspects Variants in the Texts by F. M. Dostoevsky)

2020 ◽  
Vol 81 (2) ◽  
pp. 56-63
Author(s):  
S. A. Karpukhin
Keyword(s):  
Traditional Method ◽  
Contextual Analysis ◽  
Verb Aspect ◽  
Component Theory ◽  
Two Component ◽  
Artistic Interpretation ◽  
New Perspective ◽  
First Time ◽  
Selection Of ◽  
Aspectual Meaning

The article considers the competition of verbal aspects from a new perspective. Instead of employing the traditional method of demonstrating this phenomenon — an empirical replacement of the aspect of a verb in a phrase with the opposite — the author examines Dostoevsky’s choice between the variants found in different manuscripts of the same text. For the first time, based on a two-component theory of the semantic invariant of a verb type, the aspectual meaning of the selection of a verb aspect is revealed and, as a result of contextual analysis, an artistic interpretation of the selected type is proposed.

EFFECT OF WEB BASED INSTRUCTIONS ON ACHIEVEMENT IN SOCIAL STUDIES

2017 ◽  
Vol 4 (24) ◽  
Author(s):  
Rajwinder Kaur
Keyword(s):  
Social Studies ◽  
Conventional Method ◽  
Traditional Method ◽  
Statistical Techniques ◽  
Control Group ◽  
Web Based ◽  
Significant Difference ◽  
Post Test ◽  
Based Instruction ◽  
Experimental Group

The purpose of the study was to study the Effect of Web based instructions on achievement in Social Studies. The sample consisted of 100 students from Grade 8th of schools affiliated to C.B.S.E of Ferozepur and were randomly split into two groups-control (taught by traditional method) and experimental (taught by Web based instruction) groups. Firstly pre-test was administered on both the groups; then the students in the control group were taught by conventional method while experimental group was taught by Web based instructions. Then post-test was administrated on both the groups. The Statistical techniques were then employed to data collected and analysis and interpretation of the data was done. The result of the study implied that there exists significant difference in achievement in Social Studies based on Web based instructions and conventional method. The study also revealed that there exist significant gender differences in achievement in Social Studies with Web based instructions.

scholarly journals Molecular Detection and Phylogeny of Anaplasmaphagocytophilum and Related Variants in Small Ruminants from Turkey

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 814
Author(s):  
Münir Aktaş ◽  
Sezayi Özübek ◽  
Mehmet Can Uluçeşme
Keyword(s):  
16S Rrna ◽  
Small Ruminants ◽  
Rrna Gene ◽  
Infection Rates ◽  
Sheep And Goats ◽  
Significant Difference ◽  
Pcr Rflp ◽  
First Time ◽  
Japan And China ◽  
Apparently Healthy

Anaplasma phagocytophilum causes tick-borne fever in small ruminants. Recently, novel Anaplasma variants related to A. phagocytophilum have been reported in ruminants from Tunisia, Italy, South Korea, Japan, and China. Based on 16S rRNA and groEL genes and sequencing, we screened the frequency of A. phagocytophilum and related variants in 433 apparently healthy small ruminants in Turkey. Anaplasma spp. overall infection rates were 27.9% (121/433 analyzed samples). The frequency of A. phagocytophilum and A. phagocytophilum-like 1 infections was 1.4% and 26.5%, respectively. No A. phagocytophilum-like 2 was detected in the tested animals. The prevalence of Anaplasma spp. was comparable in species, and no significant difference was detected between sheep and goats, whereas the prevalence significantly increased with tick infestation. Sequencing confirmed PCR-RFLP data and showed the presence of A. phagocytophilum and A. phagocytophilum-like-1 variant in the sampled animals. Phylogeny-based on 16S rRNA gene revealed the A. phagocytophilum-like 1 in a separate clade together with the previous isolates detected in small ruminants and ticks. In this work, A. phagocytophilum-like 1 has been detected for the first time in sheep and goats from Turkey. This finding revealed that the variant should be considered in the diagnosis of caprine and ovine anaplasmosis.

Ultrastructural studies of Chlamydia psittaci 6BC variant strains. I. Ultrastructure of the surface layers of egg-passaged 6BC strain

1973 ◽  
Vol 19 (8) ◽  
pp. 887-894
Author(s):  
Linda Poffenroth ◽  
J. W. Costerton ◽  
Nonna Kordová ◽  
John C. Wilt
Keyword(s):  
Chick Embryo ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Chlamydia Psittaci ◽  
Gram Negative Bacteria ◽  
Surface Layers ◽  
Electron Microscopic ◽  
Gram Negative ◽  
Ultrastructural Studies ◽  
First Time

Electron microscopic examination of a semipurified Chlamydia psittaci 6BC strain attenuated in chick embryo yolk sac revealed for the first time two morphologically distinct small elementary bodies which differ both in the ultrastructure of their surface layers and in their buoyant densities in sucrose gradients. Also, the morphology of the surface layers of the larger reticulate forms in cell-free systems is described for the first time. Many points of difference between the surface envelopes and internal structure of chlamydial particles and those of Gram-negative bacteria are discussed.

scholarly journals Effect of Mosses on physical and chemical properties of soil in temperate forests of Garhwal Himalayas

2021 ◽  
pp. 1-10
Author(s):  
Anshu Siwach ◽  
Siddhartha Kaushal ◽  
Ratul Baishya
Keyword(s):  
Physical Properties ◽  
Chemical Properties ◽  
Nutrient Status ◽  
High Rate ◽  
Physical And Chemical Properties ◽  
Garhwal Himalayas ◽  
Forest Sites ◽  
Significant Difference ◽  
Physical And Chemical ◽  
And Chemical Properties

Abstract Mosses are one of the most important and dominant plant communities, especially in the temperate biome, and play a significant role in ecosystem function and dynamics. They influence the water, energy and element cycle due to their unique ecology and physiology. The present study was undertaken in three different temperate forest sites in the Garhwal Himalayas, viz., Triyuginarayan (Kedarnath Wildlife Sanctuary (KWLS)), Chakrata, and Kanasar forest range. The study was focused on understanding the influence of mosses on soil physical properties and nutrient availability. Different physico-chemical properties were analysed under two different substrata, that is, with and without moss cover in two different seasons, viz., monsoon and winter. We observed mosses to influence and alter the physical properties and nutrient status of soil in both seasons. All soil physical and chemical properties, except magnesium, showed significant difference within the substrates, among all the sites and across the two seasons. Besides the soil characteristics underneath the moss vegetation, the study also highlights the diversity of mosses found in the area. Mosses appear to create high nutrient microsites via a high rate of organic matter accumulation and retain nutrients for longer periods thus, maintaining ecosystem stability.

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