Letter To The Editor

PEDIATRICS ◽  
1970 ◽  
Vol 45 (1) ◽  
pp. 158-158
Author(s):  
E. Richard Stiehm
Keyword(s):  
Cord Blood ◽  
Caproic Acid ◽  
Serum Samples ◽  
Blood Samples ◽  
Letter To The Editor

In our study, "Fibrin Split Products in the Serum of Newborns" (Pediatrics, 43: 770, 1969) , we did not use thrombin or a fibrinolytic inhibition in the collection of our serum samples for the fibrin split product (FSP) determinations; in retrospect, this is a deficiency in our study. Since receiving the foregoing letters, 12 additional cord blood samples were studied, collected with and without the addition of .5 cc of .1M epsilon amino caproic acid (EACA) and/or 100 units of thrombin per 10 ml.

Letter To The Editor

PEDIATRICS ◽  
1970 ◽  
Vol 45 (1) ◽  
pp. 155-155
Author(s):  
Judith M. Chessells ◽  
W. R. Pitney
Keyword(s):  
Cord Blood ◽  
Hemagglutination Inhibition ◽  
Sodium Citrate ◽  
Newborn Infants ◽  
Caproic Acid ◽  
Healthy Newborn ◽  
Letter To The Editor ◽  
High Incidence ◽  
Inhibition Technique

Drs. Stiehm and Clatanoff (Pediatrics, 43: 770, 1969) report a high incidence of split products of fibrin (SPF) in the cord blood of healthy newborn infants. This is contrary to our own experience which had indicated the presence of SPF in only 5% of healthy newborns. We use a hemagglutination inhibition technique which is at least as sensitive as the tube precipitin assay employed by Drs. Stiehm and Clatanoff. The two methods differ in one important aspect, however; we have tested cord blood drawn into a mixture of sodium citrate and epsilon amino-caproic acid to inhibit in vitro fibrinolysis.

scholarly journals Determinants and Measurement of Neonatal Vitamin D: Overestimation of 25(OH)D in Cord Blood Using CLIA Assay Technology

2019 ◽  
Vol 105 (4) ◽  
pp. e1085-e1092
Author(s):  
Mengdi Lu ◽  
Bruce W Hollis ◽  
Vincent J Carey ◽  
Nancy Laranjo ◽  
Ravinder J Singh ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cord Blood ◽  
Late Pregnancy ◽  
First Trimester ◽  
Controlled Study ◽  
Positive Bias ◽  
Third Trimester ◽  
Serum Samples ◽  
Human Cord Blood ◽  
Blood Samples

Abstract Context Vitamin D (VD) deficiency in pregnancy and the neonatal period has impacts on childhood outcomes. Maternal VD sufficiency is crucial for sufficiency in the neonate, though the effect of early versus late pregnancy 25-hydroxy-vitamin D (25(OH)D) levels on neonatal levels is unknown. Furthermore, chemiluminescence immunoassays (CLIAs) are widely used, though their validity in measuring 25(OH)D specifically in cord blood specimens has not been established. Objective To assess the validity of a CLIA in the measurement of cord blood 25(OH)D and to evaluate maternal determinants of neonatal 25(OH)D, including early versus late pregnancy 25(OH)D levels. Design This is an ancillary analysis from the Vitamin D Antenatal Asthma Reduction Trial (VDAART), a randomized, double-blinded, placebo-controlled study. Participants and Intervention A total of 881 pregnant women at high risk of having offspring asthma were randomized to receive VD supplementation or placebo. Serum samples were collected from mothers in early and late pregnancy and from offspring cord blood at birth. 25(OH)D levels were assayed by CLIA in all maternal and offspring samples and by LC-MS/MS in all offspring samples and a subset of 200 maternal third trimester samples. Results Cord blood 25(OH)D levels were higher as measured by CLIA (mean 37.13 ng/mL [SD 18.30]) than by LC-MS/MS (mean 23.54 ng/mL [SD 11.99]), with a mean positive bias of 13.54 ng/mL (SD 12.92) by Bland-Altman analysis. This positive bias in measurement by CLIA was not observed in maternal samples. Third trimester 25(OH)D was a positive determinant of neonatal 25(OH)D levels. Conclusion Chemiluminescence immunoassays overestimate 25(OH)D levels in human cord blood samples, an effect not observed in maternal blood samples. The quantification of 25(OH)D by CLIA should therefore not be considered valid when assayed in cord blood samples. Third trimester, but not first trimester, maternal 25(OH)D is one of several determinants of neonatal 25(OH)D status.

scholarly journals Antibodies to Pneumococcal Proteins PhtD, CbpA, and LytC in Filipino Pregnant Women and Their Infants in Relation to Pneumococcal Carriage

2009 ◽  
Vol 16 (6) ◽  
pp. 916-923 ◽  
Author(s):  
Emma Holmlund ◽  
Beatriz Quiambao ◽  
Jukka Ollgren ◽  
Teija Jaakkola ◽  
Cécile Neyt ◽  
...  
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Vaccine Candidate ◽  
Geometric Mean ◽  
Protein A ◽  
Nasopharyngeal Swab ◽  
Serum Samples ◽  
Blood Samples ◽  
Clear Cut ◽  
Pneumococcal Carriage

ABSTRACTThis study focuses on the immunogenicity of the following three pneumococcal vaccine candidate proteins in Filipino infants, all inducing protection in animal models: pneumococcal histidine triad protein D (PhtD), choline binding protein A (CbpA), and the lysozyme LytC. The immunoglobulin G antibody concentrations to PhtD, its putative, protective, and exposed C-terminal fragment (PhtD C), CbpA, and LytC were measured by enzyme immunoassay in 52 serum samples from pregnant women, 39 cord blood samples, and consecutive serum samples (n= 263) from 52 newborns between 6 weeks and 10 months of age scheduled to be taken at six time points. A nasopharyngeal swab to detect pneumococcal carriage was taken parallel to the serum samples. The antibody concentrations in the cord blood samples were similar to those in the samples from the mothers. In infant sera, the geometric mean antibody concentrations (GMCs) for all three proteins decreased until the age of 18 weeks and started to increase after that age, suggesting that the infants' own antibody production started close to the age of 4 to 5 months. The increase in GMCs by age, most clear-cut for CbpA, was associated with pneumococcal carriage. Anti-PhtD concentrations were higher than anti-PhtD C concentrations but correlated well (rof 0.89 at 10.5 months), suggesting that antibodies are directed to the supposedly exposed and protective C-terminal part of PhtD. Our results show that young children are able to develop an antibody response to PhtD, CbpA, and LytC and encourage the development of pneumococcal protein vaccines for this age group.

scholarly journals Mother-Infant Transfer of Anti-Human Papillomavirus (HPV) Antibodies following Vaccination with the Quadrivalent HPV (Type 6/11/16/18) Virus-Like Particle Vaccine

2012 ◽  
Vol 19 (6) ◽  
pp. 881-885 ◽  
Author(s):  
Katie Matys ◽  
Sara Mallary ◽  
Oliver Bautista ◽  
Scott Vuocolo ◽  
Ricardo Manalastas ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cord Blood ◽  
International Study ◽  
Maternal Blood ◽  
Hpv Infection ◽  
Maternal Serum ◽  
Serum Samples ◽  
Double Blind ◽  
Blood Samples ◽  
Vaccine Type

ABSTRACTThe exploratory immunogenicity objective of this analysis was to characterize the titer of vaccine human papillomavirus (HPV)-type immunoglobulins in both peripartum maternal blood and the cord blood of infants born to women who received blinded therapy. Data were derived from a randomized, placebo-controlled, double-blind safety, immunogenicity, and efficacy study (protocol 019; NCT00090220). This study enrolled 3,819 women between the ages of 24 and 45 years from 38 international study sites between 18 June 2004 and 30 April 2005. Data in the current analysis are from subjects enrolled in Philippines and Thailand. For each of HPV types 6, 11, 16, and 18, maternal anti-HPV was found in cord blood samples. Furthermore, HPV titers in cord blood samples were highly positively correlated with maternal HPV titers. Additionally, there were instances when anti-HPV antibodies were no longer detectable in maternal serum samples and yet were detected in matched cord blood samples. These results demonstrate that quadrivalent HPV (qHPV) vaccine-induced antibodies cross the placenta and could potentially provide some benefit against vaccine-type HPV infection and related diseases such as recurrent respiratory papillomatosis.

scholarly journals PRRSV detection by qPCR in processing fluids and serum samples collected in a positive stable breeding herd following mass vaccination of sows with a modified live vaccine

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
A. Lebret ◽  
P. Berton ◽  
V. Normand ◽  
I. Messager ◽  
N. Robert ◽  
...  
Keyword(s):  
The United States ◽  
Live Vaccine ◽  
Mass Vaccination ◽  
Respiratory Syndrome Virus ◽  
Serum Samples ◽  
Blood Samples ◽  
Stability Monitoring ◽  
Breeding Herd ◽  
Modified Live Vaccine ◽  
Processing Fluid

AbstractIn the last two decades, in France, Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) stabilization protocols have been implemented using mass vaccination with a modified live vaccine (MLV), herd closure and biosecurity measures. Efficient surveillance for PRRSV is essential for generating evidence of absence of viral replication and transmission in pigs. The use of processing fluid (PF) was first described in 2018 in the United States and was demonstrated to provide a higher herd-level sensitivity compared with blood samples (BS) for PRRSV monitoring. In the meantime, data on vertical transmission of MLV viruses are rare even as it is a major concern. Therefore, veterinarians usually wait for several weeks after a sow mass vaccination before starting a stability monitoring. This clinical study was conducted in a PRRSV-stable commercial 1000-sow breed-to-wean farm. This farm suffered from a PRRS outbreak in January 2018. After implementing a stabilisation protocol, this farm was controlled as stable for more than 9 months before the beginning of the study. PF and BS at weaning were collected in four consecutive batches born after a booster sow mass MLV vaccination. We failed to detect PRRSV by qPCR on PF and BS collected in a positive-stable breeding herd after vaccination with ReproCyc® PRRS EU (Boehringer Ingelheim, Ingelheim, Germany).

Production of dendritic cells and cytokine-induced killer cells from banked umbilical cord blood samples

2015 ◽  
Vol 2 (11) ◽  
Author(s):  
Phuc Van Pham ◽  
Binh Thanh Vu ◽  
Viet Quoc Pham ◽  
Phong Minh Le ◽  
Hanh Thi Le ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Killer Cells ◽  
Blood Samples

Search for Melanoma Markers in Plasma and Serum Samples

2005 ◽  
Vol 11 (3) ◽  
pp. 353-360 ◽  
Author(s):  
Roberta Seraglia ◽  
Susanna Vogliardi ◽  
Graziella Allegri ◽  
Stefano Comai ◽  
Mario Lise ◽  
...  
Keyword(s):  
High Frequency ◽  
Healthy Subjects ◽  
Ionic Species ◽  
Low Molecular Weight ◽  
Ionization Mass ◽  
Plasma Samples ◽  
Serum Samples ◽  
Blood Samples ◽  
Melanoma Patients ◽  
Diagnostic Ions

Fourteen blood samples from patients with melanomas and 11 blood samples from healthy subjects were analyzed by matrix-assisted laser desorption/ionization mass spectrometry. The study focussed on species of low molecular weight, in the 800–5000 Da range, present in plasma and sera. While for healthy subjects plasma samples lead to the production of a higher number of ionic species, for melanoma patients a high number of diagnostic ions, present with high frequency and with quite high relative abundance, are present, in particular, in serum samples and, to a lesser extent, also in plasma. Since plasma samples are obtained more easily in comparison to sera, it is possible to suggest that plasma can also be used for these studies.

Comparison of Maternal Sera, Cord Blood, and Neonatal Sera for Detecting Presumptive Congenital Syphilis: Relationship With Maternal Treatment

PEDIATRICS ◽  
1993 ◽  
Vol 91 (1) ◽  
pp. 88-91
Author(s):  
Rakesh S. Chhabra ◽  
Luc P. Brion ◽  
Martha Castro ◽  
Lawrence Freundlich ◽  
Joy H. Glaser
Keyword(s):  
Cord Blood ◽  
Prenatal Exposure ◽  
Congenital Syphilis ◽  
Multiple Comparisons ◽  
Serologic Test ◽  
Blood Samples ◽  
The Past ◽  
Maternal Treatment ◽  
Treatment Administration

The incidence of congenital syphilis has increased rapidly over the past few years. Most infected mothers and their newborns are asymptomatic at birth and diagnosis depends on serologic testing during pregnancy and at delivery. This study was initiated to compare maternal sera, cord blood, and neonatal sera for detecting presumptive congenital syphilis and to assess the role of maternal treatment (administration of penicillin to the mother at least 1 month before delivery) on the serologic results at the time of delivery. The serologic results from all live deliveries complicated by a positive maternal and/or neonatal test for syphilis during a 12-month period were compared using χ2 analysis and multiple comparisons for proportions. Of 3306 livebirths, 73 (2.2%) were complicated by a positive maternal or neonatal serology. At delivery, the serologic test was positive in 68 (94%) of 72 maternal sera, 30 (50%) of 60 cord sera, and 43 (63%) of 68 neonatal sera. In the absence of maternal treatment, 95% of the maternal sera, 66% of the cord blood samples, and 86% of the neonatal sera were positive. If the mother had been treated, 94% of maternal sera, 36% of cord sera, and 39% of neonatal sera were positive. Cord blood and neonatal sera appear to be inferior to maternal sera for detecting prenatal exposure to syphilis. Cord serology is also inferior to neonatal serology at 2 to 3 days of age. The most effective way to identify newborns at risk for congenital syphilis is to obtain a maternal serologic diagnosis during pregnancy and to test maternal and neonatal sera at delivery.

CAPACITY OF THYROXINE-BINDING GLOBULIN TO BIND TRIIODOTHYRONINE AND THYROXINE IN MATERNAL AND CORD BLOOD

PEDIATRICS ◽  
1962 ◽  
Vol 29 (3) ◽  
pp. 369-375
Author(s):  
William M. Michener ◽  
W. Newlon Tauxe ◽  
Alvin B. Hayles
Keyword(s):  
Cord Blood ◽  
Normal Values ◽  
Binding Capacity ◽  
Quantitative Difference ◽  
Maternal Blood ◽  
Blood Samples ◽  
Thyroxine Binding Globulin

Normal values for the measurement of thyroidal function using the erythrocytic uptake of I131-labeled triiodothyronine and the thyroxine-binding capacity of the inter-alpha globulin were established. Paired maternal and cord blood samples collected at the time of delivery were studied with these methods. The erythrocytic uptake of labeled hormone was increased in cord blood as compared to maternal blood. Cord blood apparently binds exogenous triiodothyronine in a different manner than it does exogenous thyroxine. Whether this is a qualitative or quantitative difference was not shown in this study.

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