Leukocyte Lactate Dehydrogenase in Bacterial Meningitis

PEDIATRICS ◽  
1974 ◽  
Vol 54 (1) ◽  
pp. 27-33
Author(s):  
David W. Powers ◽  
Elia M. Ayoub
Keyword(s):  
Lactate Dehydrogenase ◽  
Bacterial Meningitis ◽  
Newborn Infants ◽  
Neonatal Meningitis ◽  
Subdural Effusion ◽  
Upper Limit ◽  
Ldh Activity ◽  
Initiation Of Therapy ◽  
Peripheral Leukocytes ◽  
Normal Controls

Lactate dehydrogenase (LDH) was assayed in peripheral leukocytes of 32 children with bacterial meningitis, nine children with nonbacterial meningitis, and 24 age-matched, healthy controls. Leukocyte LDH activity in patients with bacterial meningitis was significantly higher than in patients with nonbacterial meningitis (p < 0.01) and normal controls (p < 0.001). Eighty-eight percent of the patients with bacterial meningitis had leukocyte LDH values exceeding, 150 units/106 WBC, the upper limit of normal value for controls. Nitroblue tetrazolium (NBT) dye tests performed on 23 patients with bacterial meningitis were elevated in ten patients (43%). The elevation of leukocyte LDH activity in patients with bacterial meningitis appears to be independent of the number of circulating granulocytes or the presence of bacteremia. A major factor which appeared to influence the leukocyte LDH activity was the duration of adequate antibiotic therapy. Sequential determinations showed a return of the LDH values to normal approximately ten days after the initiation of therapy. A rise in leukocyte LDH was encountered in two patients with subdural effusion. The finding of significantly elevated leukocyte LDH values in three newborn infants with neonatal meningitis and sepsis suggests that this test may be of value in the diagnosis of neonatal sepsis.

Use of Limulus Lysate for Detecting Gram-Negative Neonatal Meningitis

PEDIATRICS ◽  
1976 ◽  
Vol 58 (1) ◽  
pp. 105-109
Author(s):  
Donald Dyson ◽  
George Cassady
Keyword(s):  
Bacterial Meningitis ◽  
Rapid Detection ◽  
Newborn Infants ◽  
Blood Cultures ◽  
Neonatal Meningitis ◽  
Gram Positive ◽  
Specific Test ◽  
Gram Negative ◽  
Gram Negative Organisms ◽  
Limulus Test

The Limulus, lysate test was evaluated as a method for rapid detection of neonatal gram-negative bacterial meningitis. A total of 208 CSF samples were collected from 145 newborn infants suspected of having meningitis. Initial samples from all six babies with culture-proven gram-negative bacterial meningitis had positive Limulus tests within 30 minutes of incubation. Samples from 14 infants with gram-negative organisms isolated only in blood and/or urine, as well as from four neonates with gram-positive organisms in CSF cultures and from 13 with gram-positive organisms in blood cultures all yielded negative Limulus tests. Thus, of 37 newborn infants with bacteria demonstrated in systemic cultures, only those six with gram-negative organisms in CSF had positive CSF Limulus tests. The CSF Limulus test was shown to be a rapid, reliable, and specific test for the detection of neonatal gram-negative bacterial meningitis.

scholarly journals MODELING THE EFFECTS OF LEAD POLLUTION ON LACTATE DEHYDROGENASE ACTIVITY

10.23856/3305 ◽  
2019 ◽  
Vol 33 (2) ◽  
pp. 50-55
Author(s):  
Olena Konovalova
Keyword(s):  
Heavy Metals ◽  
Lactate Dehydrogenase ◽  
Energy Metabolism ◽  
Lead Acetate ◽  
Lead Pollution ◽  
Lactate Dehydrogenase Activity ◽  
Influence Mechanism ◽  
Ldh Activity ◽  
Small Doses ◽  
Experimental Substantiation

The changes of activity of LDH and its isoenzymatic fractions under load with lead acetate are described. The introduction of a subacute dose of Pb2 + led to a decrease in the LDH activity of the rat liver and an increase in the heart. Preliminary introduction of small doses of Pb2 + partially prevents increased LDH activity in the heart and completely inhibits its increase in the liver. The proportion of urea-stable fraction in the heart and blood serum is significantly increased, indicating an increase in the aerobic direction of energy metabolism. The obtained results are the experimental substantiation of further investigations of influence mechanism of heavy metals on the organism, as well as the motivation to prevent environmental pollution by man-made xenobiotics.

scholarly journals In Vivo Enzymes Activities of Some Ru(II) Compounds with N-Alkylphenothiazines

2016 ◽  
Vol 66 (4) ◽  
pp. 497-508
Author(s):  
P. Milena Krstić ◽  
Z. Sunčica Borozan ◽  
P. Sofija Sovilj ◽  
R. Sanja Grgurić-Šipka ◽  
M. Jelena Oljarević
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Normal Activity ◽  
Protective Effects ◽  
Toxic Compounds ◽  
Physiological Conditions ◽  
Positive Effects ◽  
Ldh Activity

Abstract The purpose of the present study was to investigate and compare the effects of two ruthenium complexes with trifluoperazine on acethylcholinesterase enzyme activity and lactate dehydrogenase levels in vivo under physiological conditions in rats blood. Complexes 1 and 2 showed positive effects on acethylcholinesterase at all doses and did not disturb its normal activity. Total LDH activity was inhibited in the presence of both complexes, but Ru(II) complexes showed different effects on the activity of LDH isoenzymes. The activities of LDH1 and LDH2 isoenzymes were decreased in all applied doses of the complex 2, while the activity of LDH2 reduced using complex 1 in the same doses. Results of the present study suggest the neuro- and cardio protective potential of oral administration of complexes 1 and 2, as non-toxic compounds under physiological conditions. These protective effects are the result of their potent antioxidant activity.

Early Development of Children With Abnormalities of the Sex Chromosomes: A Prospective Study

PEDIATRICS ◽  
1974 ◽  
Vol 54 (2) ◽  
pp. 208-212
Author(s):  
Martha F. Leonard ◽  
Gail Landy ◽  
Frank H. Ruddle ◽  
Herbert A. Lubs
Keyword(s):  
Mental Retardation ◽  
Sex Chromosomes ◽  
Language Skills ◽  
Newborn Infants ◽  
Genetic Constitution ◽  
Developmental Potential ◽  
Cytogenetic Studies ◽  
Development Of Language ◽  
A Prospective Study ◽  
Normal Controls

Out of cytogenetic studies of 4,400 consecutive newborn infants, 11 were found to have abnormalities of the sex chromosomes. None had distinguishing clinical abnormalities. These children, with ten normal controls, were evaluated at 1 and at 2½ years by child development specialists who were not informed as to which children had abnormal karyotypes. None of the children was mentally defective, but the control children showed a trend toward slightly higher developmental quotients than the study children. Of all sectors of development, language was the most affected, with language skills considerably delayed in the study children compared with the controls by the age of 2½. Further studies on these children are planned. Up to the age of at least 2½ years, abnormality of sex chromosomes was not found to cause mental retardation or specific personality deviations. It may be associated with increased vulnerability, with its greatest impact on the development of language. However, a range of developmental levels and personality has been demonstrated in these eleven children, and one cannot predict developmental potential from a knowledge of the genetic constitution.

SUBDURAL EFFUSIONS COMPLICATING BACTERIAL MENINGITIS

PEDIATRICS ◽  
1951 ◽  
Vol 7 (1) ◽  
pp. 34-43 ◽  
Author(s):  
MARGARET H. D. SMITH ◽  
RICHARD E. DORMONT ◽  
GEORGE W. PRATHER
Keyword(s):  
Bacterial Meningitis ◽  
Early Recognition ◽  
Acute Bacterial Meningitis ◽  
High Survival ◽  
Subdural Effusion ◽  
Older Patient ◽  
Subdural Hygroma ◽  
Treatment Regimens ◽  
Prolonged Fever ◽  
Subdural Effusions

Twenty cases of subdural effusion are presented occurring in infants under the age of 14 months during their convalescence from acute bacterial meningitis due to H. influenzae type b, D. pneumoniae, paracolon bacillus and Ps. aeruginosa. The incidence of these effusions must be quite high, since at least 20 out of 43 cases of meningitis under the age of 2 years had fluid in the subdural space at some time during their hospital stay. The authors believe that subdural taps should be performed on admission for diagnostic purposes whenever the patient has been treated with antibiotics before coming to the hospital, and has no organisms in the spinal fluid at the time he is first seen, as well as in all patients who give evidence of prolonged fever, focal neurologic signs, convulsions, etc., following meningitis. The trauma incident to subdural taps is probably not itself responsible for causing subdural effusions, since in most cases fluid was present at the first tap. The matter of age incidence needs investigation. There is no reason to believe that such effusions are limited to infants. The possible presence of a subdural effusion should be considered in an older patient whose convalescence from meningitis is slow, accompanied by prolonged fever, headaches and any suggestive focal neurologic signs. The authors' experience, as well as a review of the older literature, indicates that from the confusing clinical entities known as subdural hygroma, pachymeningitis hemorrhagica interna, etc., one group can be singled out as occurring on the basis of an underlying infection. Its frequent occurrence in recent years is owing to the high survival rate in bacterial meningitis. The actual pathogenesis of this lesion; the incidence of loculated fluid; the incidence of "membranes"; the possible influence of different treatment regimens on the occurrence of this syndrome; the possibility suggested by analogy with "pleuropneumonia" that subdural effusions, when infected, may serve as foci for later recurrences of meningitis; the possible influence of early recognition and treatment of subdural effusions on the ultimate prognosis in meningitis are matters for the future to determine.

scholarly journals Complete Genome Sequence of the Neonatal Meningitis-Causing Escherichia coli Strain NMEC O18

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Bryon A. Nicholson ◽  
Yvonne M. Wannemuehler ◽  
Catherine M. Logue ◽  
Ganwu Li ◽  
Lisa K. Nolan
Keyword(s):  
Escherichia Coli ◽  
Bacterial Meningitis ◽  
Neonatal Mortality ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Coli Strain ◽  
Neonatal Meningitis ◽  
Neurologic Sequelae ◽  
Highly Virulent

Neonatal meningitis Escherichia coli (NMEC) is a common agent of neonatal bacterial meningitis, causing high neonatal mortality and neurologic sequelae in its victims. Here, we present the complete genome sequence of NMEC O18 (also known as NMEC 58), a highly virulent (O18ac:K1, ST416) strain.

scholarly journals Clinical Profile and Outcome of Acute Encephalitis Syndrome in Dhulikhel Hospital of Nepal

2013 ◽  
Vol 32 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Srijana Dongol ◽  
Shreema Shrestha ◽  
Narayan Shrestha ◽  
J Adhikari
Keyword(s):  
Hearing Loss ◽  
Bacterial Meningitis ◽  
Sensorineural Hearing Loss ◽  
Clinical Signs ◽  
Viral Meningitis ◽  
Sensorineural Hearing ◽  
Subdural Effusion ◽  
Eosinophilic Meningitis ◽  
Acute Encephalitis Syndrome ◽  
Acute Encephalitis

Introduction: Acute encephalitis syndrome (AES) is a constellation of clinical signs and or symptoms i.e. acute fever with acute change in mental status. AES may be present as encephalitis, meningoencephalitis or meningitis. It can be associated with severe complication, including impaired consciousness, seizure, limb paresis or death. Materials and Methods: Study consisted of retrospective analysis of hospital records of children up to 16 years of age admitted with diagnosis of AES in the department of Paediatrics Dhulikhel Hospital, Kathmandu University Teaching Hospital, Dhulikhel Kavre from January 2010 to December 2011. Results: During the two years (January 2010 to December 2011), 47 patients of AES were admitted. Among the admitted cases there were 34 male and 13 female patients. Meningitis cases were 29, encephalitis cases were 14 and 4 meningoencephalitis cases. Among the meningitis cases, viral meningitis accounted for 12, bacterial meningitis accounted for 15 and 1 tubercular meningitis.One was eosinopilic meningitis in which the causative organism was found to be fasciolosis by ELISA. Viral encephalitis was found to be the most common cause of encephalitis. Sensorineural hearing loss was seen in 3 cases, subdural effusion in 1 and hydrocephalus in 1. One patient had intracranial hemorrhage with hemiparesis as a complication of eosinophilic meningitis. Conclusion: Acute encephalitis syndrome is one of the most common causes of PICU admission in Dhulikhel hospital. Bacterial meningitis was common among the acute encephalitis syndrome followed by viral meningitis. One case of eosinophilic meningitis with intracranical hemorrhage and hemiparesis was found. Sensorineural hearing loss was found to be commonest complication. DOI: http://dx.doi.org/10.3126/jnps.v32i3.6683 J. Nepal Paediatr. SocVol.32(3) 2012 201-205

scholarly journals Urinary Lactate Dehydrogenase Activity and Its Isozyme Patterns in Kawasaki Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Yoichi Kawamura ◽  
Seiichiro Takeshita ◽  
Takashi Kanai ◽  
Mari Takizawa ◽  
Yusuke Yoshida ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Kawasaki Disease ◽  
Pattern Analysis ◽  
Upper Urinary Tract ◽  
Potential Significance ◽  
Lactate Dehydrogenase Activity ◽  
Isozyme Patterns ◽  
Ldh Activity ◽  
Sterile Pyuria ◽  
Ldh Isozymes

Abnormal urinary findings, such as sterile pyuria, proteinuria, and microscopic hematuria, are often seen in the acute phase of Kawasaki disease (KD). We investigated the potential significance of urinary lactate dehydrogenase (U-LDH) activity and its isozyme patterns in KD. Total U-LDH activity and its isozymes (U-LDH1-5) levels were compared among 120 patients with KD, 18 patients with viral infection (VI), and 43 patients with upper urinary tract infection (UTI) and additionally compared between intravenous immunoglobulin (IVIG) responders (n=89) and nonresponders (n=31) with KD. Total U-LDH activity was higher in KD (35.4±4.8 IU/L, P<0.05) and UTI patients (66.0±8.0 IU/L, P<0.01) than in VI patients (17.0±6.2 IU/L). In the isozyme pattern analysis, KD patients had high levels of U-LDH1 and U-LDH2, while UTI patients had high levels of U-LDH3, U-LDH4, and U-LDH5. Furthermore, IVIG nonresponders of KD had significantly higher levels of total U-LDH activity (45.1±4.7 IU/L, P<0.05), especially U-LDH1 and U-LDH2 (P<0.05), than IVIG responders (32.0±2.8 IU/L). KD patients have increased levels of total U-LDH activity, especially U-LDH-1 and U-LDH2, indicating a unique pattern of U-LDH isozymes different from that in UTI patients.

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