scholarly journals Effect of alcohol tincture of Aralia elata on the organism of rats and their gut microbiota against the background of excessive fat diet

2019 ◽  
Vol 10 (4) ◽  
pp. 497-506 ◽  
Author(s):  
V. V. Brygadyrenko ◽  
M. A. Lieshchova ◽  
M. V. Bilan ◽  
N. M. Tishkina ◽  
A. V. Horchanok
Keyword(s):  
Gut Microbiota ◽  
Biologically Active ◽  
Male Rats ◽  
Laboratory Animals ◽  
Relative Mass ◽  
Physical Work ◽  
Stress Effect ◽  
Physical Work Capacity ◽  
Total Calcium ◽  
Aralia Elata

Pharmacological effects of the medicinal plant Aralia elata (Miq.) Seem. 1868 (Araliaceae) are related to presence of over 150 secondary metabolites, including flavonoids, sterols, polysaccharides, terpenoid saponins and terpenoid acids, though the main biologically active substances of A. elata are saponins and flavonoids. Some clinical tests and experimental studies have proved the influence of A. elata on the organism through increase in physical work capacity, exerting an anti-stress effect against a broad range of harmful factors, including stress from cold, immobilization, ultraviolet radiation and low pressure. The literature also reports the anti-ulcer, anti-secretory, antifungal, anti-tumour, and antimicrobial activity of preparations from this plant. In our laboratory experiment, we determined that ethanolic tincture of A. elata has no effect on the intensity of growth of body weight of young rats against the background of excessive content of fat in their diet. Excessive fat-feeding of male rats leads mostly to disorders in the functioning of the liver and development of steatosis. This was accompanied by reduction in relative mass of the liver, increase in activity of hepatic enzymes, dysproteinemia, increase in the level of bilirubin and decrease in the level of urea. Additional use of 0.1% ethanol led to impaired functioning of the kidneys, reduction of their relative mass, signs of dehydration, increase in the level of creatinine and total calcium in the blood. Use of ethanolic tincture of A. elata mitigates negative excess of fat, is accompanied by normalization of indices of mass of the organs, less notable dysproteinemia, impairment in the level of creatinine, glucose, urea, cholesterol, bilirubin and total calcium. Ethanolic tincture of A. elata has a low immunosuppressive action, against the background of a high fat diet it leads to increase in the amount of typical Escherichia сoli, decrease in Еnterococcus spp. and Enterobacter spp., significant decrease and in high concentrations (0.1% ethanolic tincture of A. elata) elimination of bacteria of Clostridium and Klebsiella genera, and also various yeast fungi in the intestine. In the examined male rats, against the background of excess of fat in the diet, no serious changes in the composition of the normal gut microbiota (Bifidobacterium spp., Lactobacillus spp., Proteus spp., Staphylococcus spp., Candida spp.) was observed, nor were any lactose-negative enterobacteria (Citrobacter genus) found. Perspectives of further research include determining histological, histochemical and immune-histological changes in the organs of laboratory animals under the effect of ethanolic tincture of A. elata following excessive accumulation of fat.

scholarly journals Combined effect of glyphosphate, saccharin and sodium benzoate on rats

2018 ◽  
Vol 9 (4) ◽  
pp. 591-597 ◽  
Author(s):  
M. A. Lieshchova ◽  
N. M. Tishkina ◽  
A. A. Bohomaz ◽  
P. M. Gavrilin ◽  
V. V. Brygadyrenko
Keyword(s):  
Body Weight ◽  
Sodium Benzoate ◽  
Food Additives ◽  
Combined Effect ◽  
Male Rats ◽  
Laboratory Animals ◽  
Relative Mass ◽  
Control Group ◽  
Expiry Date ◽  
The Impact

Herbicides and food additives are included in many food products for humans. Non-used products or products beyond their expiry date are deposited in places of utilizatioin of solid municipal wastes, where they can take effects on the organisms of mouse-like rodents. Among the herbicides, glyphosphate takes first place in the world for volume of production, and is the most intensely used in agricultural farming. The discussion about negative impact on the organisms of mammals, especially against the background of using various substances and environmental factors, continues. In this study, we determined the combined effect of glyphosphate and food additives on the organism of laboratory animals, which manifested in changes in body weight, condition and indices of mass of the internal organs and blood parameters. Four groups of laboratory male rats were formed, which over 42 days received: unlimited access to clean water; 1% aqueous solution of glyphosate; 1% solution of glyphosphate and 1% solution sodium benzoate; 1% solution of glyphosphate with 1% solution of saccharin. Glyphosphate and glyphosphate with sodium benzoate and saccharin significantly reduced the daily increases in body weight of animals compared to the control group. The studied substances have notable suppressive effect on the immune system and haematopoiesis in general, which is manifested in reduce of relative mass of the thymus and spleen against the background of increase in the amount of lymphocytes in the peripheral blood. The inhibition of haematopoiesis is indicated by decrease in the amount of erythrocytes, neurophils and hemoglobin of blood of animals from the experimental groups. The impact on the digestive system of glyphosphate and food additives is indicated by occurance of the effect of “irritation” of mucous membranes, and, as a result, disorders in absorption followed by the disorder in metabolic processes. A dysbalance occurs in enzymic systems of the organism, which is manifested in distrophic processes, especially in the liver parenchyma, indicated by the activity of blood enzymes (ALT, AST, alkaline phosphatase), total number and ratio of proteins of blood plasma. We determined the impact of glyphosphate and its mixes with benzoate and saccharin on the pancreas, which manifests in severe pancreatitis with steep increase in the level of glucose of blood. The results of the study allow us to state that mixture of glyphosphate and food additives can cause toxic effect in animals and humans, which often contact with herbicides.

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

scholarly journals Lower extremity muscle size and strength and aerobic capacity decrease with caloric restriction but not with exercise-induced weight loss

2007 ◽  
Vol 102 (2) ◽  
pp. 634-640 ◽  
Author(s):  
Edward P. Weiss ◽  
Susan B. Racette ◽  
Dennis T. Villareal ◽  
Luigi Fontana ◽  
Karen Steger-May ◽  
...  
Keyword(s):  
Weight Loss ◽  
Caloric Restriction ◽  
Aerobic Capacity ◽  
Lean Mass ◽  
Muscle Volume ◽  
Muscle Size ◽  
Thigh Muscle ◽  
Physical Work ◽  
Physical Work Capacity ◽  
Exercise Induced

Caloric restriction (CR) results in fat loss; however, it may also result in loss of muscle and thereby reduce strength and aerobic capacity (V̇o2 max). These effects may not occur with exercise-induced weight loss (EX) because of the anabolic effects of exercise on heart and skeletal muscle. We tested the hypothesis that CR reduces muscle size and strength and V̇o2 max, whereas EX preserves or improves these parameters. Healthy 50- to 60-yr-old men and women (body mass index of 23.5–29.9 kg/m2) were studied before and after 12 mo of weight loss by CR ( n = 18) or EX ( n = 16). Lean mass was assessed by dual-energy X-ray absorptiometry, thigh muscle volume by MRI, isometric and isokinetic knee flexor strength by dynamometry, and treadmill V̇o2 max by indirect calorimetry. Both interventions caused significant decreases in body weight (CR: −10.7 ± 1.4%, EX: −9.5 ± 1.5%) and lean mass (CR: −3.5 ± 0.7%, EX: −2.2 ± 0.8%), with no significant differences between groups. Significant decreases in thigh muscle volume (−6.9 ± 0.8%) and composite knee flexion strength (−7.2 ± 3%) occurred in the CR group only. Absolute V̇o2 max decreased significantly in the CR group (−6.8 ± 2.3%), whereas the EX group had significant increases in both absolute (+15.5 ± 2.4%) and relative (+28.3 ± 3.0%) V̇o2 max. These data provide evidence that muscle mass and absolute physical work capacity decrease in response to 12 mo of CR but not in response to a similar weight loss induced by exercise. These findings suggest that, during EX, the body adapts to maintain or even enhance physical performance capacity.

Physiological and psychological factors associated with onset of high-altitude headache in Chinese men upon acute high-altitude exposure at 3700 m

Cephalalgia ◽  
2016 ◽  
Vol 37 (4) ◽  
pp. 336-347 ◽  
Author(s):  
Wen-Yun Guo ◽  
Shi-Zhu Bian ◽  
Ji-Hang Zhang ◽  
Qian-ning Li ◽  
Jie Yu ◽  
...  
Keyword(s):  
Risk Factors ◽  
High Altitude ◽  
Mood State ◽  
Physical Work ◽  
Internal Diameter ◽  
Physical Work Capacity ◽  
P Values ◽  
Factors Associated ◽  
Diastolic Velocity ◽  
Independent Risk Factors

Aim We aimed to identify clinical characteristics and risk factors associated with onset of high-altitude headache (HAH) after acute exposure at 3700 m. Method In two hours, 163 individuals ascended by plane to 3700 m. Demographic information, physiological and psychological measurements, cognitive function, physical work capacity tests and profile of mood states within one week prior to the departure and within 24 hours after arrival were examined. Results HAH patients featured significantly higher vertebral artery diastolic velocity (Vd), heart rate (HR) and pulmonary artery diameter. HAH was also associated with a more negative mood state, including scores for tension anxiety, depression, hostility, fatigue and confusion, as well as lower vigor (all p values <0.05). Furthermore, negative emotions were positively related to HAH severity. HAH slightly decreased cognitive functioning. HR, Vd, lack of vigor, confusion and self-reported anxiety (all p values <0.05) were independent risk factors for HAH. We have identified three independent baseline predictors for HAH including internal diameter of the left ventricle (LVD), Athens Insomnia Scale (AIS) and confusion score. Conclusions Higher HR, Vd, confusion and self-reported anxiety and insufficient vigor were independent risk factors for HAH. Furthermore, higher baseline LVD, AIS and confusion score are independent predictors of HAH.

Physical work capacity (VO2 max) and work ability (WAI) of active employees (men and women) in Poland

2005 ◽  
Vol 1280 ◽  
pp. 156-160 ◽  
Author(s):  
J. Bugajska ◽  
T. Makowiec-Dąbrowska ◽  
A. Jegier ◽  
A. Marszałek
Keyword(s):  
Work Ability ◽  
Work Capacity ◽  
Physical Work ◽  
Physical Work Capacity ◽  
Vo2 Max ◽  
Men And Women

Physiological responses to maximal effort wheelchair and arm crank ergometry

1980 ◽  
Vol 48 (6) ◽  
pp. 1060-1064 ◽  
Author(s):  
R. M. Glaser ◽  
M. N. Sawka ◽  
M. F. Brune ◽  
S. W. Wilde
Keyword(s):  
Pulmonary Ventilation ◽  
Lactate Concentration ◽  
Blood Lactate Concentration ◽  
Correlation Coefficients ◽  
Work Capacity ◽  
Peak Oxygen Uptake ◽  
Physical Work ◽  
Physical Work Capacity ◽  
Maximal Heart Rate ◽  
Peak Vo2

The purpose of this investigation was to compare physical work capacity (PWC), peak oxygen uptake (peak VO2), maximal pulmonary ventilation (VEmax), maximal heart rate (HRmax), and maximal blood lactate concentration (LAmax) for wheelchair ergometer (WERG) and arm crank ergometer (ACE) exercise. For this, wheelchair-dependent (n = 6) and able-bodied (n = 10) subjects completed a progressive intensity, discontinuous test for each mode of exercise. Each test was terminated by physical exhaustion and/or an inability to maintain a flywheel velocity of 180 m.min-1. Relatively high correlation coefficients were found between values obtained during the two modes of ergometry for PWC, peak VO2, VEmax, and HRmax. WERG exercise was found to elicit a significantly (P less than 0.05) lower PWC (by 36%), HRmax (by 7%), and LAmax (by 26%) than ACE exercise. Peak VO2 and VEmax, however, were similar for both exercise modes. These data suggest that either exercise mode may be used for fitness testing and training of people who cannot use their legs and that arm cranking may be a superior method to propel wheelchairs.

Lifting Physical Work Capacity as a Function of Frequency

1987 ◽  
Vol 31 (12) ◽  
pp. 1331-1335
Author(s):  
Jeffrey E. Fernandez ◽  
Robert J. Marley ◽  
Nancy B. Stubbs
Keyword(s):  
Laboratory Experiment ◽  
Work Capacity ◽  
Task Design ◽  
Physical Work ◽  
Physical Work Capacity ◽  
Frequency Range ◽  
Lifting Technique ◽  
Male Subjects

A laboratory experiment using 15 male subjects was conducted to document lifting physical work capacity over the frequency range of 2 to 12 lifts per minute and to compare these values to bicycling physical work capacity. Results indicate that bicycling PWC is significantly higher than lifting PWC at the 0.05 level. A variation in lifting PWC as a function of frequency was observed. This could be attributed to lifting technique. Task design should, therefore, not only consider lifting PWC but also the frequency of lift.

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