scholarly journals Association of BoLA-DRB3.2 alleles with fusobacteriosis in cows

2020 ◽  
Vol 11 (2) ◽  
pp. 249-254
Author(s):  
T. M. Suprovych ◽  
M. P. Suprovych ◽  
R. V. Kolinchuk ◽  
T. M. Karchevska ◽  
I. O. Chornyi ◽  
...  
Keyword(s):  
Immune Response ◽  
Molecular Genetic ◽  
Special Role ◽  
Exon 2 ◽  
Black And White ◽  
Pcr Rflp ◽  
Close Relationship ◽  
Class Ii Antigens ◽  
Dairy Breed ◽  
Selection Of

The Major Histocompatability Complex (MHC) determines the immune response to pathogens, and its genes are promising candidates for the search of associations with diseases. A special role is played by BoLA-DRB3 gene, the product of which directly participates in the binding of alien antigens and conditions the specificity of the immune response. The second exon of this gene codes β1-domain of class II antigens, which is necessary for binding a broad spectrum of alien antigens. Exon 2 of BoLA-DRB3 gene is extremely polymorphic, giving the possibility to search the associations of its alleles with various diseases. The article provides the results of the study on polymorphism of alleles of BoLA-DRB3.2 gene for detection of its associations with sensitivity to fusobacteriosis (necrobacteriosis) of cows. The survey was performed using PCR-RFLP method with DNA of blood from 176 cows of two herds of Ukrainian black-and-white dairy breed. As a result of the studies, in the first herd, 25 BoLA-DRB3.2 alleles were found. In the selections of nectobacteriosis susceptible and resistant cows, we found 22 and 21 variants respectively. In the second herd, in the general selection and group of healthy animals, 27 alleles were typed, and 22 in the group of susceptible cows. BoLA-DRB3.2*22 allele was the commonest in both herds in both general selections and groups of nectobacteriosis-resistant cows. In the selection of susceptible animals, the commonest was the variant BoLA-DRB3.2*16. We determined statistically significant associations of BoLA-DRB3.2 alleles with sensitivity to nectobacteriosis of cattle. BoLA-DRB3.2*03 and *22 alleles associate with nectobacteriosis-resistant, while *16 and *23 – with nectobacteriosis-susceptible cows of the both studied groups. Also, in the first herd, another allele was found – *24, indicating close relationship with the disease. The studies of polymorphism of BoLA-DRB3 gene expand the knowledge about genetic peculiarities of the Ukrainian black-and-white dairy breed. The identified molecular-genetic markers could be useful for breeders whose work is oriented towards the formation of herds which are resistant to diseases of the limbs in cattle.

scholarly journals FEATURES OF DIAGNOSIS OF NECROBACTERIOSIS OF COWS BY PCR-RFLP

2020 ◽  
pp. 26-37
Author(s):  
O.D. Biriukova ◽  
T.M. Suprovych ◽  
M.P. Suprovych ◽  
S.V. Laiter-Moskaliuk ◽  
I.O. Chornyi
Keyword(s):  
Molecular Genetic ◽  
Experimental Sample ◽  
Exon 2 ◽  
Blood Samples ◽  
Restriction Fragments ◽  
Black And White ◽  
Pcr Rflp ◽  
Dairy Breed ◽  
Established Technique ◽  
Gene Exon

Molecular genetic markers can detect polymorphism at the DNA level. This feature determines the possibility of their widespread use in genetics and breeding. Alleles of the BoLA-DRB3 gene (exon 2) can act as such markers if a statically significant association between the disease and the allele is established. The presence of such DNA markers in the genotype of animals makes it possible to judge the likelihood of disease in postnatal ontogenesis immediately after the birth of a heifer, based on which we can conclude about the conditions of further use of the animal in the main herd. According to the results of studying the polymorphism of the BoLA-DRB3 gene in cows of the Ukrainian black and white dairy breed resistant and susceptible to necrobacteriosis, four "informative" alleles were revealed. Two of them *03 and *22 are associated with resistance, and the other two - *16 and *23 with susceptibility to necrobacteriosis. The presence of these alleles in the genotype of the animal is determined by testing performed by PCR-RFLP. The method is time consuming, labor intensive and costly. To simplify it, the following technique is proposed. Restriction fragments of alleles *03, *16, *22 and *23 for endocluases RsaI, XhoII and HaeIII have the following DNA patterns: bbb, jbd, mba and nba. Due to the peculiarity of the restriction fragments, which is that endonuclease XhoII reveals in these alleles only one pattern b with length of 284 bp, the process of determining informative alleles can be simplified. Isolation of DNA from blood samples and amplification of a fragment of the BoLA-DRB3.2 gene with a size of 284 bp is carried out according to the established technique. Next, the restriction of the fragment by endonuclease XhoII and sampling having a pattern b. Selected samples are treated with RsaI endonuclease and only those with patterns b, j, m and n remain. The next step is to restrict the selected samples with HaeIII endonuclease and select heifers with bbb (*03) and nba (*23) genotypes. After the first restriction, blood samples without pattern b are eliminated from the experimental sample; after the second – two alleles with patterns RsaI + XhoII jb (*16) and mb (*22) are unambiguously determined, after the third – genotypes bbb and nba, which correspond to alleles *03 and *23. In total, only 75% of blood samples are typed, which reduces the material consumption, time and cost of work to identify heifers genetically susceptible (resistant) to necrobacteriosis.

scholarly journals Evaluation of the response of Turkey strains to Salmonella and Newcastle vaccines

2021 ◽  
pp. 1-9
Author(s):  
Ekei Victor Ikpeme
Keyword(s):  
Immune Response ◽  
Antibody Titre ◽  
Research Work ◽  
Haemagglutination Inhibition ◽  
Resistance Breeding ◽  
Laboratory Assessment ◽  
Blood Samples ◽  
Black And White ◽  
Disease Resistance Breeding ◽  
Selection Of

This research work was carried out to evaluate the immune response of 3 turkey strains to salmonella and Newcastle vaccines measured via antibody titre. The study deployed a total of 120 poults (40 black, 40 white and 40 lavender) strains. To ascertain the initial antibody titre of all the birds, blood samples were obtained from their wing veins immediately after acclimatization for salmonella and Newcastle antibody titre.  The test for salmonella and Newcastle antibody titre responses of the birds were performed using widal and haemagglutination inhibition (HI), respectively. The birds were divided into two broad groups tagged as A and B, which represented Salmonella and Newcastle vaccines, respectively. Each group had 3 sub-groups denoting the 3 strains of turkey used for the study. After vaccination, blood samples were collected from all the birds at 3 and 5 days for laboratory assessment of antibody titre response. The results showed that there was significant increase (p<0.05) in the antibody titre response of all the turkey strains after vaccination. Results obtained on the time of exposure of the birds to vaccine revealed that higher antibody titre values were obtained from the black and white turkey strains at 3 days than at 5 days after administration of Newcastle vaccine, except in the lavender strain, which had higher titre value at 5 days after Newcastle vaccination. Following administration of salmonella vaccine, black and lavender strains had statistically higher antibody titre response (2.03 and 2.10 mean loge widal, respectively) than white strain (1.86 mean loge widal). Similarly, the black strain also had higher antibody titre value when Newcastle vaccine was administered (2.35 mean loge HI) followed by lavender (1.99 mean loge HI) and white strain (1.71 mean loge HI). Black strains showed more antibody titre response and by implication, stronger immunity to Salmonella and Newcastle vaccines. More importantly, the differential response of the 3 turkey strains to vaccine could give turkey breeders the choice of selection of turkey breeds for disease resistance breeding.

scholarly journals THE PREDICTED GENETIC PROGRESS IN DAIRY CATTLE POPULATIONS USING A VARIETY OF METHODS FOR EVALUATION AND SELECTION OF ANIMALS

2018 ◽  
Vol 51 ◽  
pp. 27-34 ◽  
Author(s):  
O. I. Babenko ◽  
V. P. Оleshko ◽  
V. Y. Afanasenko
Keyword(s):  
Dairy Cattle ◽  
Milk Yield ◽  
Milk Fat ◽  
Genetic Improvement ◽  
Holstein Cattle ◽  
Genetic Progress ◽  
Conventional Breeding ◽  
Black And White ◽  
Dairy Breed ◽  
Selection Of

Genetic progress in a herd of animals is due to the selection of four categories of pedigree animals: fathers of sires, mothers of sires, fathers of cows and mothers of cows. Extremely important role in genetic improvement of a herd plays selection of the sires for insemination of breeding stock which become the potential parents of cows. The selection of sires’ mothers, sires’ and cows’ parents provides 90-95% of the effect of selection in animal population, and massive selection of cows’ mothers only 5-10%. The main selection trait in а herd is milk production, therefore with the purpose of analyzing the effectiveness of selection by this trait defined pedigree value of 4 categories of pedigree animals, which influenced on the pace of improvement of the populations. Therefore, to accelerate genetic improvement of dairy cattle populations is necessary to increase the magnitude of the genetic benefits of parental animals and reduced generation intervals, which is the aim of our research. The average annual genetic progress through selection of four categories of pedigree animals in the herd of Holstein breed is 61.2 kg of milk per cow per year and the pace of genetic improvement of the herd – 0.81 %. In the population of Ukrainian Black-and-White Dairy breed, these figures are respectively 43.2 kg of milk and the pace of genetic improvement of the herd – 0.62 % Much more opportunities in raising the rate of genetic improvement can be achieved through the introduction of genomic (GS) or marker-assisted selection (MAS). Under the terms of the genomic breeding, final estimation of pedigree value of sires is carried out during 2.5 years in the contrast with traditional breeding, which involves the time for 5 years. Experts estimate the cost from the use of GS or MAS compared with conventional breeding are up to 92%, and the efficiency of selection is improved two times. Taking into account that data of productivity of animals are associated with genes of economically useful traits, the topical issue is the selection of the optimal polymorphic marker systems for their introduction into practice of breeding and assessment of genetic structure of cattle breeds. On the basis of our results of studies of the effect of QTL – gene, the complex model genotype for the selection of animals for the purpose of increasing milk yield in animals of Ukrainian Black-and-White Dairy breed is like this: CSNSАВ, GHLL, βLGАВ, Pit-1АВ; Holstein – CSNS AB, GHLV, βLGАA, Pit-1АВ. Comparison of model genotypes of Ukrainian Black-and-White Dairy and Holstein shows that there are differences in the combination of genotypes contributing to the best development of traits. Thus, the mass fraction of the protein, the model genotypes are identical at 75% in both breeds: by the yield, quantity of milk fat and protein and total milk fat and protein model genotypes match by 50%. Due to implementation of genomic evaluation and breeding of dairy cattle, the valuation period of pedigree animals was reduced significantly. Unlike conventional breeding, the introduction of genomic selection will reduce the genetic interval in the population of Ukrainian Black-and-White Dairy breed of sires’ fathers by 4.5 years, sires’ mothers by 4.6 years, cows’ fathers by 4.6 years and cows’ mothers by 2.9 years. In the population of Holstein cattle generation interval of sires’ fathers is reduced by 4.3 years, sires’ mothers by 4.5 years, cows’ fathers by 4.5 years, and cows’ mothers by 2.7 years. The results of our studies show that the rate of genetic improvement of dairy cattle population in the main breeding traits can be improved through the selection of animals by QTL-complexes. Thus, the selection of breeding stock based on a comprehensive model of genotype on yield gave an opportunity to improve pedigree value of the maternal cows at 155 kg compared to traditional breeding, and in the population of Holstein cattle at 246 kg. Only these factors allow genetic progress in milk yield in the population of Ukrainian Black-and-White Dairy cattle by 128.5 kg, which is 2.9 times more than the value of the actual genetic progress and in the population of Holstein cattle – 183.2 kg, which is three times more compared to the actual value of genetic progress for milk yield in this population. The contribution of the cows’ mothers to genetic progress increases. So, if at conventional breeding, the contribution of this category of pedigree animals was negative (-4.9%), selection on QTL complex is 3.2% and in the population of Holstein cattle 12%. If you apply the selection of other categories of pedigree animals (sires’ fathers, mothers and parents of sires) on QTL-complexes, which contribution in the genetic improvement of populations is 88.0–96.8%, the rate of genetic improvement of dairy cattle populations will greatly increase. Conclusion. To accelerate genetic improvement of dairy cattle populations it is necessary to use molecular genetic markers that control metabolic processes in the body.

Use of the Molecular-Genetic Markers in the Selection Process of the Ukrainian Animal Husbandry

2014 ◽  
Vol 1 (2) ◽  
pp. 24-32 ◽  
Author(s):  
K. Kopylov ◽  
K. Kopylova ◽  
A. Shelov ◽  
O. Berezovsky
Keyword(s):  
Selection Process ◽  
Molecular Genetic ◽  
Rflp Analysis ◽  
Animal Husbandry ◽  
Complex Model ◽  
Black And White ◽  
Long Term Storage ◽  
National Bank ◽  
Genetic Examination ◽  
Dairy Breed

Aim. To analyze the genetic structure of the Holstein and Simmental breeding bulls from various breeding stock-rearing farms of Ukraine by polymorphisms of kappa-casein, β-lactoglobulin, growth hormone, leptin, pituitary-specifi c transcription factor, myostatin loci genes. Methods. Individual genotypes of the animals were determined using the polymerase chain reaction (PCR) followed by determination of restriction fragment length polymorphism (RFLP analysis). Results. A complex model genotype for increasing milk yield of the animals of the Ukrainian Black and White Dairy breed – κ-Cn АВ , βLG АВ , GH LV , Pit-1 АА , LEP АА , butterfat content – κ-Cn АВ , βLG АВ , GH VV , Pit-1 АА , LEP BB/AB ; Ukrainian Red and White Dairy breed – Cn АА , βLG АА , GH L , PIt-1 AB and κ-Cn AA , βLG AB , GH LV , Pit-1 AB ; Simmental breed – κ-Cn ВВ , βLG ВВ , GH LL , LEP AB and κ-Cn ВВ , βLG ВВ , GH LV , LEP AA was determined respectively. Genetic certifi cation of the breeding bulls of 25 various breeds on standard microsatellite panels (ВМ1824, ВМ2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, ETH225, ETH3) was held for their biological material long-term storage rationale in the National Bank of Genetic Resources. Conclusions. Comprehensive monitoring of cattle breeding resources predetermines the implementation of genetic examination of the breeding animals’ origin according to ISAG international guidelines in Ukraine. This will determine the specifi c type or breed gene pool as a whole, also characterize the vector of micro-evolutionary processes in the populations of animals and allow to solve other breeding and genetic problems.

scholarly journals FERTILITY AND PRODUCTIVITY OF FIRST-CALF COWS OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED

2019 ◽  
Vol 57 ◽  
pp. 79-86 ◽  
Author(s):  
D. М. Kucher ◽  
A. М. Didkovsky
Keyword(s):  
Milk Production ◽  
Protein Production ◽  
Reproductive Capacity ◽  
Lactation Period ◽  
Milk Productivity ◽  
Black And White ◽  
Fertility Index ◽  
Service Period ◽  
Dairy Breed ◽  
Selection Of

The selection of animals at the lowest number of indicators is more effective compared to selection on many grounds. The main focus during the selection of Holstein cattle was to increase milk productivity, which meant the neglect of other important features, such as fertility (fecundity) of animals. The purpose of the work was to determine the level of milk productivity of cows of Ukrainian Black-and-White dairy breed in conditions of a particular farm, in which there would be a successful combination of reproductive capacity support for adjusting the selection of animals. The research was carried out in the breeding plant of the Ukrainian Black-and-White dairy breed of farm "Khliborob" of Kozyatinsky district of Vinnytsia region. The material of the research was the information of 212 first-calf cows of Ukrainian Black-and-White dairy breed of this farm. Indicators of dairy productivity were studied in 305 days of lactation according to zootechnical records. Reproductive ability of cows evaluated index insemination, age 1 st calving (months), duration (days) of service period, the period of pregnancy, the period between calving, the dry period, a rate of reproductive capacity (CRF), the calves are emitted per 100 cows (%) and the fertility index. The first-breed differentiation was carried out at a deviation of 0.7 σ from the mean of index (M) of the fertility index. Such a division makes it possible to divide the herd into 3 groups in a ratio of 1:2:1. The highest level of dairy productivity have characterized by animals of the I group with low fertility (the fertility index was on average 40.8), which significantly surpassed their peers of the III group with good fertility for the livelihood for 305 days of lactation for 434 kg, milk protein production – by 15.2 kg, total milk fat and protein production – by 26.0 kg (P ≤ 0.05). The increase in the duration of lactation and deterioration of fertility has associated with an increase in the duration of the service period, which is quite natural, since animals need more time to invade the genital organs and normalize their functioning and restore normal sexual cycle. The difference in the duration of the lactation period when comparing animals with different fertility in all cases was high-reliable at P ≤ 0.001 (td = 3.38–6.00). The effect of cow fertility on the duration of their lactation was ηx = 0.160 (at P ≤ 0.001). The main indicator that characterizes the economic efficiency of the industry is the profit received from the cow due to the receipt of the calfs and milk. Taking into account the duration of the service period, which exceeded the optimum terms, in the first days of the experimental groups, indicators of non-compliance of products in the form of calves per 100 cows were determined, which made 18.5%, 14.1% and 11.4% respectively. The force of influence on the fertility of cows at the age of their first calving was ηx = 0.507 (at P ≤ 0.001). According to the received data, the first-calf cows with a tiredness of 6274 kg of milk showed the highest calving output in the herd is for 100 cows (89.6%) and reproductive capacity (0.91). The lowest calf output (82.5%) and reproductive capacity (0.84) was found in cows with a milk yield of 6708 kg for 305 days of lactation. As the milk productivity increases, the duration of the service period extends from 123.1 to 158.3 days (r = 0.16 ± 0.07, at P ≤ 0.05), and the index of insemination – from 2.35 to 3,02 (r = 0.17 ± 0.08, at P ≤ 0.05). The relationship was straightforward and reliable. It is necessary to focus on improving cow fertility, which is the basis of effective milk production. For the successful management of dairy cattle breeding in the conditions of the "Hliborob" farm, we recommend to select the herds of heifers to be retrieved from animals with satisfactory fertility, since this will be economically beneficial for the farm and physiologically optimal for animals. A negative correlation was detected between milk productivity and reproductive capacity - with an increase in the fertility index per unit of hopes for cows, it would increase by 148.5 kg of milk. Under existing conditions of feeding and keeping on the farm it is expedient to select for further breeding of animals with a productivity level of 6.5 thousand kg of milk for 305 days of lactation. For a more complete realization of the genetic potential of milk production of cows of Ukrainian Black-and-White milk, it is necessary to improve the conditions of their feeding, maintenance.

scholarly journals DETERMINATION OF ALLELES OF BOLA-DRB3.2 GENE ASSOCIATED WITH NECROBACTERIOSIS OF THE COWS OF UKRAINIAN BLACK-AND-WHITE DAIRY CATTLE

2018 ◽  
Vol 51 ◽  
pp. 205-213 ◽  
Author(s):  
T. Suprovich ◽  
Т. Karchevska ◽  
R. Kolinchuk ◽  
V. Mizyk
Keyword(s):  
Relative Risk ◽  
Dairy Cows ◽  
Dna Markers ◽  
Total Sample ◽  
Exon 2 ◽  
Black And White ◽  
Pearson Criterion ◽  
Wide Range ◽  
Close Relationship ◽  
High Level

The main objective of research "BoLA and disease" is the need to develop approaches and obtain reliable criteria which would allow to judge about animal genetic predisposition to the disease and about change of its immunological status in the development of pathological process. Genes of class II of main histocompatibility complex have the greatest association to diseases. Now 54 alleles of BoLA-DRB3.2 have been described by PCR-RFLP. The high level of allelic diversity of the gene is caused by necessity of tying a wide range of foreign antigens, which leads to the possibility of its use as a marker for various diseases of cattle. This article presents the results of detecting alleles of BoLA-DRB3.2 gene, which have the expressed relationship with the disease of Ukrainian Black-and-White Dairy cows on necrobacteriosis and can be used as DNA markers of this disease. Diagnosis of necrobacteriosis was set at the basis of clinical, pathological and epizootic data and laboratory results. The blood samples were taken from 114 cows, 43 of which had the disease. Spectrum of alleles of exon 2 of BoLA-DRB3 gene was studied by PCR. 54 alleles were determined in total. Alleles, which have a close relationship with susceptibility or resistance to necrobacteriosis and can be used as DNA markers, were established on indicators of frequency and relative risk (RR) with test on Pearson criterion (χ2). 32 alleles were determined in the experimental group of animals. There were seven alleles with a frequency greater than 5%. The most often determined allele of BoLA-DRB3.2 was *24. It is present in 18% of the animals. And often determined alleles were *22 (7,9%) and *28 (7,5%). Limit higher than 5% was for alleles *08 and * 09 (6,1%), *03 and *16 (5,3%). The lowest frequency of detection was for alleles *06, *25, *31 and *41 (0,4%). Alleles of BoLA-DRB3.2*24 (16,9%), *22 (10,6%), *28 (8,5%), *03 (7,7%), *08 and *10 (6,3%) were often determined in the group of healthy cows. Alleles *06, *14, *19, *25 and *51 weren’t determined in this group. The animals with necrobacteriosis had often alleles *24 (19,8%), *16 (12,8%), *23 (8,1%), *8, *10 and *28 (5,8%). Alleles *01, *11, *21, *31 and *41 weren’t in general. In the three experimental groups 8 alleles were determined with a frequency of over 5% (all herd, healthy and diseased animals respectively). There are four alleles among them presented in all three samples: *08, *10, *24 and *28. Two "informative" alleles (*03 and *22) were found in every the 20th animal simultaneously in two groups of cows – healthy animals and in the total sample. Also two "informative" alleles *16 and *23 were simultaneously in the diseased cows and in the total sample. 11 alleles have significant association with susceptibility or resistance to necrobacteriosis on criterion of relative risk. There are 4 alleles *16 (24,1%), * 18 (5,25%), *25 (5,04%) and *23 (4,41%), indicating the relationship with disease (RR ≥ 2). Four alleles of BoLA-DRB3.2 are significant on criterion χ2 and have a sufficient test of validity for the studied biological objects. Allele *16 shows a very high level test of validity P = 0,999 (χ2 = 16,6). Three alleles *03 (4,93), *23 (4,86) and *22 (4,03) have a minimum acceptable test of validity for χ2 for P = 0,95. 8 alleles: *3 (-7,7), *21 (- ,44), *36 (-3,87), *22 (-3,57), *12 (-3,18), *1 and *11 (-3,13) and *26 (- 2,51) indicate neсrobaсteriosis resistance (RR ≤ -2). Allele would be associated with the disease if the condition performed RR ≥ 2 і χ2> 3,8. There are two such alleles: *16 (RR = 24,1; χ2 = 16,6), *23 (RR = 4,41; χ2 = 4,86). Also "negative" alleles on risk of disease manifest are *18 (5,25) and *25 (5,08), but with insufficient validity of Pearson criterion (respectively 2,45 and 1,66). Allele would be associated with the resistance to disease if the condition performed RR ≤ -2 і χ2> 3,8. There are 2 alleles associated with resistance to necrobacteriosis: *03 (RR = -7,7; χ2 = 4,93) and *22 (RR = -3,57; χ2 = 4,03). Also six alleles (* 01, * 11, * 12, * 21, * 26 and * 36) detected resistance to neсrobacteriosis on high level of relative risk, but with insufficient validity. It should be noted, that allele BoLA-DRB3.2*22, which proved to be a "positive" marker of resistance to necrobacteriosis, has a strong correlation with resistance to mastitis in cows of Ukrainian Black-and-White Dairy (RR = -2,52; χ2 = 5,02) and Ukrainian Red-and-White Dairy breeds (RR = -4,66; χ2 = 11,11) in previous studies. The study of the distribution of alleles of exon 2 of BoLA-DRB3 gene at the Ukrainian Black-and-White Dairy cows, which were healthy and diseased by necrobacteriosis, revealed the alleles which had a close relationship with penchant to this disease (* 16 and * 23) and two alleles associated with resistance (* 03 and * 22). Given the fact that the research was conducted directly on animal blood DNA the detected alleles BoLA-DRB3 should be used as DNA markers in the analysis of susceptibility or resistance to necrobacteriosis of cows.

scholarly journals VARIABILITY OF LONGEVITY TRAITS OF COWS OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED IN VARIOUS VARIANTS OF SELECTION

2019 ◽  
Vol 57 ◽  
pp. 143-151 ◽  
Author(s):  
L. M. Khmelnychyi ◽  
A. V. Loboda
Keyword(s):  
High Reliability ◽  
Statistical Significance ◽  
Black And White ◽  
Genetic Potential ◽  
Breeding Selection ◽  
The Difference ◽  
High Level ◽  
Dairy Breed ◽  
Selection Of

In modern conditions of intensive dairy farming, the first and most important task was to ensure a high level of productivity of cows with simultaneous long-term use. Taking into account the important breeding aspect of the measure for intra-and interlinear breeding, it was considered appropriate to study the efficiency of combining the lines at the breeding of animals of the Sumy intra-breed type of Ukrainian Black-and-White diary breed by traits of longevity. The research on determining the effectiveness of combining the lines at breeding of cows on grounds of duration of use and lifetime productivity was carried out in the herd of pedigree plant PE "Burynske" of Pidlisnivskyi department in Sumy district. According to the results of evaluation of cows obtained by intrabreeding selection of eight genealogical formations of the experimental herd, was found significant effect of heredity lines on indicators of longevity. According to the estimation of longevity, the offspring of four lines were the best: Valiant, Sjuprim, Starbuck and O. Aivengo. Advantage of daughter progeny of sires Valiants line with the highest longevity over the animals of lines of Montfrech, Matt, M. Chiftein and S. T. Rokita on this basis amounted to 252–526 days (P < 0.01–0.001). The next by lifetime rating was the offspring of O. Aivengo's line sires had an advantage over cows of the above lines with variability of 220–393 days (P < 0.05–0.001). The same lines (Valianta, Sjuprim, Starbuck and Aivengo) almost did not differ among themselves by the number of used lactations (4.6–4.8) and exceeded cows of remaining lines by 0.6–1.4 lactation. The difference between the maximum and minimum values was reliable at P < 0.001. The highest yield for a productive life was obtained from cows belonging to the Aivengo line 1189870, which with a high reliability of difference predominated the offspring of all estimated lines with variability from 3532 (P < 0.001; Syuprim line) to 13617 kg of milk (P < 0.001; line Montfrech). High lifetime milk yields differed also descendants of sires lines Sjuprim (29684 kg), Starbuck (26811 kg) and M. Chiftein (26516 kg). Among the crossed variants with the minimum longevity indicators, offspring derived from bulls-sires of interlinear selection ♂Montfrech × ♀Mett and the reverse cross – (♂Mett × ♀Montfrech) are distinguished. This was explained to a certain extent by the fact that continuators of these lines were only crossbred sires of Ukrainian Black-and-White diary breed. In variants using the parent lines of Monfrech and Matt with maternal lines, the successors of which are Holstein sires, longevity indicators in the offspring obtained from these crosses are improving. According to the lifetime yield of dairy fat, the highest rates were obtained from offspring with interlinear selection of those sires who have the highest lifetime yield of milk. These daughters are from the crosses ♂Starbuck × ♀Sjuprim (1197.4 kg) and ♂S. T. Rokita × ♀Aivengo (1189.6 kg). An indicator of the effectiveness of longevity was also milk yield per day of life of dairy cows. Variability in interline selection varied from 8.7 (♂Metta × ♀Monfrech) to 12.0 kg (♂S. T. Rokita × ♀Aivengo). The difference between these variants was 3.3 kg with high reliability (P < 0.001; td = 5.95). Conclusions. Identified and confirmed statistical significance regularities concerning to influence the level of traits duration of use and lifetime productivity of an alternative selection system of linear breeding convinced about feasibility of regular monitoring to assess the combination of lines in the further breeding selection cows of Sumy intrabreed type of Ukrainian Black-and-White dairy cattle. Re-selection of the best options and rejection of ineffective ones will help to increase the genetic potential of longevity traits.

scholarly journals Frequencies of genotypes and alleles of rs41256848 in the LHCGR gene in the population of black-and-white holsteinized cattle

2019 ◽  
pp. 73-76
Author(s):  
Аrina Аndranikovna Tagmazian ◽  
Anna Leonidovna Arkhipova ◽  
Artyom Vladimirovich Brigida ◽  
Eugene Aleksandrovich Klimov ◽  
Svetlana Nikolaevna Kovalchuk
Keyword(s):  
Luteinizing Hormone ◽  
Gene Encoding ◽  
Hormonal Stimulation ◽  
Black And White ◽  
Pcr Rflp ◽  
Embryo Transfer Technique ◽  
Selection Of ◽  
Stimulation Of ◽  
Donor Cows

Embryo transfer technique is one of the key in accelerated reproduction of cattle. One of the most important stages is the selection of donor cows that are most sensitive to the procedure of hormonal stimulation of ovulation. One of the promising genetic markers of the reproductive status of cattle is currently the gene encoding the luteinizing hormone / choriogonadotropin receptor (LHCGR). One of the SNP in the LHCGR gene of cattle has already been described in the literature as associated with the number of oocytes and the quality of embryos - rs41256848 (c.1401G> T, p.Trp467Cys). The purpose of this work was to estimate the frequencies of genotypes and alleles of this substitution in the population of Black-and-White holsteinized cattle (190 cows). Genotyping was performed by PCR-RFLP method. In the studied population of cattle, the frequency of the G allele associated with higher rates in the total number of oocytes and the number of embryos survived after transplantation, as well as with the least number of unfertilized oocytes, is 63.2%.  

Small Computer System for Micrograph Analysis

1981 ◽  
Vol 39 ◽  
pp. 270-271
Author(s):  
J. F. Hainfeld ◽  
J. S. Wall
Keyword(s):  
Large Range ◽  
Small Computer ◽  
Black And White ◽  
Contour Maps ◽  
Points Of Interest ◽  
Computer Aided Analysis ◽  
Specialized Hardware ◽  
Additional Memory ◽  
Selection Of ◽  
Publication Quality

Cost reduction and availability of specialized hardware for image processing have made it reasonable to purchase a stand-alone interactive work station for computer aided analysis of micrographs. Some features of such a system are: 1) Ease of selection of points of interest on the micrograph. A cursor can be quickly positioned and coordinates entered with a switch. 2) The image can be nondestructively zoomed to a higher magnification for closer examination and roaming (panning) can be done around the picture. 3) Contrast and brightness of the picture can be varied over a very large range by changing the display look-up tables. 4) Marking items of interest can be done by drawing circles, vectors or alphanumerics on an additional memory plane so that the picture data remains intact. 5) Color pictures can easily be produced. Since the human eye can detect many more colors than gray levels, often a color encoded micrograph reveals many features not readily apparent with a black and white display. Colors can be used to construct contour maps of objects of interest. 6) Publication quality prints can easily be produced by taking pictures with a standard camera of the T.V. monitor screen.

Linear type trait evaluation of firstborns of Ukrainian Black-and-White Dairy breed depending on the body build

2019 ◽  
pp. 24-34
Author(s):  
R. Stavetska ◽  
Y. Dynko ◽  
M. Bushtruk ◽  
I. Starostenko ◽  
O. Babenko ◽  
...  
Keyword(s):  
Body Condition Score ◽  
High Capacity ◽  
The Body ◽  
Body Depth ◽  
Linear Type ◽  
Body Build ◽  
Black And White ◽  
Condition Score ◽  
Type Traits ◽  
Dairy Breed

It has been experimentally proven that the strength and direction of the connection between the linear type traits evaluation of the Ukrainian black-and-white dairy breed cows and their body build depends on the type of the build and the sign of the exterior. The study was conducted in 2018 on a breeding plant of the Ukrainian Black-and-White Dairy breed SPC named Shchorsa in Kiev region (n=89). Differentiation of cows on types of their body build was conducted by the method proposed by Chernenko (low-capacity, mid-capacity and high-capacity types) and Kolesnik (by the massiveness index – on the friable and dense, the bone index – delicate and rough, the wide-breast and wide-rump indices – on narrow-body and wide-body types). It was found that the cow-heiferswere well-developed, had intermediate stature and optimum body condition score, deep body, and wide, slight slope rump. They alsohad desirable rear legs, set and foot angle but some toe-out, the udder was well-balanced. Under the good total score of the exterior of studied cows there were some animals with short stature (134– 136 cm), very wide rump, straight rear legs side view and toe-out, extremely steep foot angle, loose fore udder attachment, deep udder below hocks and front teat placement inside of quarter. The degree of development of the studied linear traits of cow-heifers confirms their in-herd variability. The variability of the exterior traits varies from 10,7 % (body depth) to 38,6 % (stature). In addition to the stature of a significant variability was characterized of angularity (22,4 %), rear legs set (22,5 %), body condition score (21,8 %), as well as traits that describe udder: rear udder attachment (31,4 %), udder depth (32,6 %) and front and rear teat position (31,3 % and 25,1 %, respectively). The high variability of linear traits indicates the possibility of effective their selection. As a result of the research, it has been established that the strength and direction of the correlations between the linear type traits evaluation of Ukrainian Black-and-White Dairy breed cows and the type of their body build depends on the type of the body build and the linear trait. The low-, mid- and high-capacity bodies build types (according to Chernenko) reliably correlate with chest width (r=0,58) and body depth (r=0,48), rear legs rear view (r=0,30), the central ligament (r=0,28), udder depth (r =-0,22) and teat length (r=0,25), P˂0,01–0,001. Friable and dense bodies build (according to Kolesnik) were most strongly associated with stature (r=-0,28), chest width (r =-0,70), and body depth (r=-0,38), as well as with rear legs rear view (r=-0,23), P˂0,05...0,001; delicate and rough bodies build – with stature (r=-0,51), angularity (r=-0,22),rump width (r=-0,35), udder depth (r=-0,27) and rear udder attachment (r =0,20), P ˂ 0,05–0,001; narrow-body and wide-body types – with chest width (r=0,30) and rump width (r=0,23), P˂0,05–0,01. Linear type trait evaluation of cows depends on the body build. The strength and direction of the correlations between types of body build and linear traits showed that the most informative is the classification of the body build by low-, mid- and high-capacity according to Chernenko – on the average η2x=17,5 %. The less informative – by narrow-body and wide-body types according to Kolesnik – η2x=7,6 %. The classification of cows on delicate-rough, narrow-body and wide-body types (according to Kolesnik) according to informativeness and reliability can have the intermediate position – η2x=10,0–11,5 %. The strongest influence on the body build has the cows’ height (η2x=27,9 % on average for all studied types of body build), chest width (η2x=33,5 %), body depth (η2x=21,8 %), rump width (η2x=24,1 %) and rear legs (η2x=18,7 %). Key words: cows, linear type trait evaluation, body build, correlation, power of influence.

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