scholarly journals Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

2021 ◽  
Vol 12 (1) ◽  
pp. 145-152
Author(s):  
H. V. Klymets ◽  
R. Y. Iskra ◽  
O. Z. Svarchevska
Keyword(s):  
Protein Metabolism ◽  
Aspartate Aminotransferase ◽  
Hematological Parameters ◽  
Aminotransferase Activity ◽  
Pregnant Rats ◽  
Pregnant Females ◽  
Group I ◽  
Γ Globulins ◽  
Group Ii ◽  
Platelet Content

Dose-dependent changes in protein metabolism in the blood and hematological parameters of pregnant rats under the effect of vanadium citrate are presented in the article. The animals were divided into five groups: group I – non-pregnant females, II – pregnant females consuming pure water without additives, III, IV, V – females which during the mating and pregnancy period received the solution of vanadium citrate at concentrations of 0.03, 0.125 and 0.50 μg V/mL water. The research findings show that in pregnant animals of group II, the level of urea and alkaline phosphatase activity increased, meanwhile aspartate aminotransferase activity decreased, as compared to the non-pregnant females of group І. The levels of total protein and albumin decreased; however, the content of β-globulins increased in the pregnant animals of group II, as compared with that in group I. Also, in the rats of group II, there was a decrease in hemolysis time, total content of erythrocytes and hemoglobin, the content of old and mature erythrocytes, while the content of young erythrocytes increased, as compared to group I. The platelet content and thrombocrit in rats of group II increased in comparison with group I. The content of leukocytes and lymphocytes in pregnant animals of group II decreased, while the content of granulocytes increased, in contrast to non-pregnant rats. Under the effect of vanadium citrate at concentrations of 0.03–0.50 μg V/mL, there was a significant increase in the maximum number of prohemolized erythrocytes, the time of maximum hemolysis was delayed by 0.4–0.6 min, as compared with the pregnant rats of group II. This did not affect the time of total hemolysis in rats of groups III and V, as compared with the pregnant animals in group II. Under the effect of vanadium citrate, an increase in the content of young erythrocytes was observed, as compared with group II. The hemoglobin content decreased at the concentration of 0.125 μg V/mL, while at the concentration of 0.50 μg V/mL it increased, as compared to the pregnant animals of group II. Also, under the effect of vanadium citrate there was a decrease in the mean hemoglobin concentration in the erythrocyte. In pregnant animals fed with vanadium citrate solutions, the platelet content and thrombocrit, the relative width of platelet distribution by volume decreased, as compared with the pregnant rats of group II. The content of leukocytes, lymphocytes and granulocytes under the effect of vanadium citrate increased, as compared with the pregnant animals in group II. Under the effect of vanadium citrate at the concentration of 0.03 μg V/mL, the level of albumin, creatinine and aspartate aminotransferase activity increased in blood plasma in comparison with group II. Meanwhile, at the concentration of 0.125 μg V/mL, the relative content of γ-globulins and aspartate aminotransferase activity increased, alkaline phosphatase activity and urea level decreased in comparison with group II. However at the concentration of 0.50 μg V/mL, the relative α- and γ-globulins content and aspartate aminotransferase activity increased, at the same time, the relative β-globulins content and urea level decreased in comparison with group II. Therefore, vanadium citrate normalizes the indicators of protein metabolism during pregnancy, thus it can be considered as a potential dietary drug for the pregnant.

scholarly journals Effects of acclimation on the haematological indices of different groups of fresh water teleosts

2015 ◽  
Vol 7 (1) ◽  
pp. 5-9 ◽  
Author(s):  
K. Roy George ◽  
N. A. Malini ◽  
D. Rajasree
Keyword(s):  
Fresh Water ◽  
Hematological Parameters ◽  
Clarias Gariepinus ◽  
Stress Factors ◽  
Basic Knowledge ◽  
Hematopoietic Tissue ◽  
Group Iii ◽  
Enhanced Production ◽  
Group I ◽  
Group Ii

Hematological parameters have been recognized as valuable tools for monitoring fish health. The aim of the present study was to obtain a basic knowledge of the hematological responses of different groups of fresh water teleosts during acclimation period. Haematological analysis was carried out in three different groups of teleosts namely Group I: Heteropneustes fossilis and Clarias gariepinus (Siluriformes), Group II: Anabas testudineus and Channa striatus (Perciformes), Group III: Labeo rohita and Cyprinus carpio (Cypriniformes). In all groups of teleosts, RBC and Hb were significantly (p<0.05) decreased after 7 days of captivity in response to stress factors raised in capture, handling and sampling procedure. WBC was found to be significantly (p<0.05) increased in group I (Siluriformes), group II (Perciformes) and group III (Cypriniformes) of teleosts after 7 days of captivity due to enhanced production of leucocytes in the hematopoietic tissue of the kidney and the spleen. RBC count and WBC count were found to be higher in H. fossilis and C. gariepinus than other groups of teleosts due to their active predacious nature. Hb values were high in 2nd group (Perciformes) consisting of A. testudineus and C. striatus by virtue of their possessions of accessory respiratory organs. PCV, MCV and MCHC were significantly (p<0.05) increased in three groups of teleosts during acclimation and values were high in second group of consisting of A. testudineus and C. striatus. MCH was higher in 3rd group of fishes consisting of L. rohita and C. carpio.

scholarly journals Protective effect of blackthorn fruits (Prunus spinosa) against tartrazine toxicity development in albino Wistar rats

2019 ◽  
Author(s):  
Igori Balta ◽  
Bogdan Sevastre ◽  
Vioara Mireșan ◽  
Marian Taulescu ◽  
Camelia Raducu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Water Mixture ◽  
Water Group ◽  
Prunus Spinosa ◽  
Food Dye ◽  
Group I ◽  
Group Ii

Background: Tartrazine (Yellow 5 or E102) is a synthetic food dye able to modify perception and behavior, causingagitation, confusion, rhinitis and can produce hyperactivity syndrome in children when is combined with benzoates.Additionally, it can trigger oxidative stress which consequently generates metabolic disorders. Therefore, the studywas designed to evaluate the harmful effects of the food additive tartrazine and to observe beneficial properties ofblackthorn fruits (Prunus spinosa) on the blood and organs of albino Wistar rats.Materials and methods: This study was carried out on 20 mature Wistar rats, randomly divided into four groupsof five animals. Over the course of the experiment, the control group received only food and drinking water, group Ireceived 75 mg of tartrazine dissolved in (250 ml) water group II was given 75 mg of tartrazine and 200 mg of driedblackthorn fruit powder 200 mg dissolved simultaneously in (250 ml) of tartrazine-water mixture (aiming to reducethe tartrazine toxicity) and group III received a higher dose of tartrazine (100 mg) in (250 ml) of water.Results: At the end of the experiment, values regarding kidney and liver weight were significantly increased, whilethe weight of the spleen was slightly decreased compared with the weight of the control group. Biochemical andhematological assays, of the blood samples show that the addition of tartrazine in the diet of rats caused significantchanges in all biochemical and hematological parameters of the blood. In the group II, which received (P. spinosa)powder combined with tartrazine, the biochemical and hematological parameters had average values similar to thecontrol group.Conclusions: Histopathological assay showed that the application of tartrazine in the group I, II and III producedlesions of the kidneys, spleen and the liver for all rodents. Tartrazine was able to generate histopathological changes,which caused significantly tissue lesions of the liver and significant changes in blood parameters. Blackthorn powdershowed a promising protective role for the blood parameters but demonstrated no significant benefits for the organs.

The changes of activity of separate carbohydrate metabolism enzymes in blood erythrocytes of pregnant rats under vanadium citrate

2019 ◽  
Vol 11 (1) ◽  
pp. 34-39
Author(s):  
Halyna Klymets
Keyword(s):  
Body Weight ◽  
Lactate Dehydrogenase ◽  
Pure Water ◽  
Female Rats ◽  
Content Increase ◽  
Pregnant Females ◽  
Group I ◽  
Metabolism Enzymes ◽  
Group Ii ◽  
Glucose 6 Phosphate Dehydrogenase

The aim of the work was to study the effect of vanadium citrate on the activity of glucose-6-phosphate dehydrogenase, lactate dehydrogenase, estradiol content and to establish correlations between the activity of the studied enzymes and the estradiol content in pregnant female rats under the effect of vanadium compound. The study was carried out on 25 female Wistar rats, divided into five groups: group I - non-pregnant, group II - pregnant consuming pure water without additives, groups III, IV, V - females which during the mating and pregnancy periods received the solution of vanadium citrate at the concentrations of 3.75, 15.63 and 62.5 μgV/kg of body weight, respectively. Glucose-6-phosphate dehydrogenase, lactate dehydrogenase and estradiol content increase in pregnant females of group II compared to group I of non-pregnant animals. In pregnant females, the effect of vanadium citrate resulted in the decrease in the activity of glucose-6-phosphate dehydrogenase and lactate dehydrogenase in the blood of animals in groups IV and V, and the content of estradiol in the animals of all three experimental groups (III, IV, V), compared to group II of pregnant females. The values of glucose-6-phosphate dehydrogenase activity in the animals of group IV, which received the solution of vanadium citrate at the concentration of 15.63 μgV/kg of body weight, was similar to that of the animals in group I. This may indicate the normalizing effect of vanadium citrate on the activity of glucose-6-phosphate dehydrogenase at the indicated concentration, which is probably due to the ability of the vanadium microelement to inhibit the activity of this enzyme. Vanadium citrate at the concentration of 15.63 μgV/kg of body weight caused the recovery of the enzyme activity to the values of the activity in group І.

Prophylactic effects of pomegranate (Punica granatum) juice on sodium fluoride induced oxidative damage in liver and erythrocytes of rats

2016 ◽  
Vol 94 (7) ◽  
pp. 709-718 ◽  
Author(s):  
Asma Bouasla ◽  
Ihcène Bouasla ◽  
Amel Boumendjel ◽  
Cherif Abdennour ◽  
Abdelfattah El Feki ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Sodium Fluoride ◽  
Hematological Parameters ◽  
Punica Granatum ◽  
Pomegranate Juice ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Standard Diet ◽  
Group I ◽  
Group Ii

The objective of this study was to investigate the protective effects of pomegranate (Punica granatum) juice (PGJ) on oxidative damages in liver tissue and erythrocytes of rats intoxicated by sodium fluoride (NaF). Rats were randomly divided into two groups: group I received standard diet and group II received orally 1 mL of PGJ. After 5 weeks of pretreatment, each group was divided again into two subgroups and treated for another 3 weeks as follows: group I was subdivided into a control group and a group that was treated with 100 ppm of NaF (in drinking water); group II was subdivided into one group that was treated daily with both 100 ppm NaF and PGJ (1 mL orally) and one that received daily 1 mL of pomegranate juice. Exposure to NaF decreased hematological parameters, changed the total protein, albumin, bilirubin levels, and increased the activities of hepatic marker enzymes. We also noted an increase in lipid peroxidation contents, accompanied by a decrease of reduced glutathione levels. Antioxidant enzyme activities in both tissues were modified in the NaF group compared with the control group. However, the administration of PGJ juice caused an amelioration of the previous parameters. Our results indicated the potential effects of NaF to induce oxidative damage in tissues and the ability of PGJ to attenuate NaF-induced oxidative injury.

scholarly journals β-Intermediate Thalasemia: triplication of genes α / β thalassemia heterozygous in Spain

ANALES RANM ◽  
2021 ◽  
Vol 138 (138(01)) ◽  
pp. 60-71
Author(s):  
Paloma Ropero ◽  
Fernando Ataulfo González Fernández ◽  
Jorge Martínez Nieto ◽  
Williana Melissa Torres Jiménez ◽  
Celina Benavente Cuesta
Keyword(s):  
Hematological Parameters ◽  
Globin Gene ◽  
Fetal Hemoglobin ◽  
Globin Genes ◽  
Thalassemia Intermedia ◽  
Transfusion Therapy ◽  
Group Iii ◽  
Group I ◽  
Caucasian Origin ◽  
Group Ii

Objectives. Check with hematological data that the diagnosis and clinical grade of β-thalassemia intermedia can be established when a triplication of genes alpha (αααanti 3.7) and heterozygous β-thalassemia are coherent. Methods. Retrospective study in which 73 patients of Caucasian origin participated, who simultaneously showed a tripling or quadrupling of the genes α and heterozygous β-thalassemia. Screening for the most frequent α-thalassemia mutations, as well as gene triplication (αααanti 3.7) was carried out by multiplex PCR followed by reverse hybridization and confirmed by MLPA. The molecular diagnosis of β-thalassemia was carried out by automatic sequencing according to the Sanger’s method. Results. Genotypes have been classified into three groups according to the number of α-globin genes and the severity of the alteration in the β-globin gene. All had a mutation in the β-globin gene (β0-thalassemia, severe β+-thalassemia, and mild β+-thalassemia). Group I patients who have inherited 6 α globin genes. Group II and group III have inherited 5 α globin genes. In group III, the patients were carriers of mutations affecting the β and δ globin genes. The most significant hematological parameters were hemoglobin levels, mean corpuscular volume, red deep width, and percentage of fetal hemoglobin. Conclusions. In group I, patients who have inherited of 6 α globin genes, either by homozygous triplication (ααα/ααα) or heterozygous quadruplication (αααα/αα), with heterozygous β-thalassemia results in severe to moderate anemia that may require transfusion therapy, being the severity of the β-globin gene mutation that would determine the clinical variation. Group II patients behaved phenotypically like mild thalassemia intermedia. Finally, group III patients behaved like a thalassemic trait since all were carriers of mutations that increase the overexpression of g genes.

scholarly journals Nephroprotective Effects of Carvedilol and Curcuma longa against Cisplatin-Induced Nephrotoxicity in Rats

2013 ◽  
Vol 5 (2) ◽  
pp. 91-98 ◽  
Author(s):  
NN Pathak ◽  
SR Rajurkar ◽  
S Tarekh ◽  
VV Badgire
Keyword(s):  
Wistar Rats ◽  
Renal Damage ◽  
Curcuma Longa ◽  
Hematological Parameters ◽  
Medical Science ◽  
Group Iii ◽  
Methanolic Extracts ◽  
Group I ◽  
Daily Dose ◽  
Group Ii

Objective: Goal of this study is to investigate the effects of carvedilol (5 mg/kg, p.o), aqueous and methanolic extract of Curcuma longa (500mg/kg, p.o) against cisplatin induced renal damage in Wistar rats after a single intraperitoneal injection of 7.5mg- 1. Methods: Wistar rats were randomly divided into 7 groups (n=10). Rats in group I were normal control received normal saline (5 ml/kg; i. p.) on day zero, while group VIa and VIb were vehicle control, received carboxymethyl cellulose (2.5 ml/kg; p.o.) and propylene glycole (2.5 ml/kg, p.o.) respectively from day 3 to 17. Renal toxicity was induced in rats of group II, III, IV and V by a single administration of cisplatin (7.5 mg/kg; i. p.) on day zero. Rats in group III, IV and V received a daily dose of carvedilol (5 mg/kg; p.o.), aqueous and methanolic extracts of Curcuma longa (500 mg/kg; p.o.) respectively from day 3 to 17, while group II served as cisplatin control. Results: Post treated rats with carvedilol and aqueous and methanolic extracts of Curcuma longa for 15 days significantly increased body weight, decreased cisplatin induced abnormalities and mortality and decreased all the kidney marker such as serum urea nitrogen (SUN), serum creatinine (SCr), total proteins (TP), and uric acid (UA) increased by cisplatin, however, no appreciable improvement in hematological parameters were observed when compared with cisplatin control. Conclusion: The results are indicative of nephroprotective effects of carvedilol as well as aqueous and methanolic extracts of Curcuma longa. DOI: http://dx.doi.org/10.3126/ajms.v5i2.5483   Asian Journal of Medical Science, Volume-5(2) 2014: 91-98

scholarly journals Immune disorders after combined treatment of obese patients with endometrial cancer

2020 ◽  
Vol 28 (4) ◽  
pp. 374-386
Author(s):  
I. S. Hromakova ◽  
P. P. Sorochan ◽  
N. E. Prokhach ◽  
M. V. Polozova ◽  
I. А. Hromakova
Keyword(s):  
Radiation Therapy ◽  
Inflammatory Process ◽  
Hematological Parameters ◽  
Relative Number ◽  
Absolute Number ◽  
Obese Patients ◽  
Immune Disorders ◽  
Group I ◽  
Normal Body Weight ◽  
Group Ii

Background. Immune disorders in antitumor treatment of obese patients may depend on the severity of the inflammatory process, which justifies the need to take into account the intensity of the latter in the analysis of complications of antitumor treatment in obese individuals. Purpose – evaluation of immune disorders after radiation therapy in obese patients with different intensity of the inflammatory process.  Materials and methods. 65 patients with stage I–II endometrial cancer underwent pangysterectomy and a postoperative course of remote gamma therapy. Examinations were performed before treatment and after radiation therapy. Subpopulation composition of lymphocytes, phagocytic activity of neutrophils, levels of circulating immune complexes and immunoglobulins G, A, M were determined by standard methods. The SF-3000 «SYSMEX» analyzer was used to evaluate hematological parameters. The level of C-reactive protein (CRP) was determined using an analyzer «RESPONS 910». Insulin and leptin levels were assessed by enzyme-linked immunosorbent assay. Results and discussion. Immune and hematological parameters were evaluated in 3 groups of patients. Group I included patients with normal body weight (BMI < 25), II and III groups consisted of obese patients (BMI > 30). Group II included patients with a CRP level below the median, which was 18,0 mg/l, group III – patients with a CRP level above 18.0 mg/l. In patients of group II there was a higher absolute and relative number of lymphocytes, the absolute number of CD3+-, CD4+- and CD8+-lymphocytes compared with those registered in groups I and III. After radiation therapy, the absolute number of lymphocytes and lymphocyte subpopulations decreased in all groups. As before treatment, higher levels of these indicators were registered in group II. In obese patients, the relative number of NK cells was lower than that determined in patients of group I before and after treatment. Conclusions. Patients with obesity and low intensity of the inflammatory process have less pronounced immune disorders after radiation therapy compared with patients who had a normal body weight and individuals with obesity and high levels of CRP.

scholarly journals HEPATOPROTECTIVE POTENTIAL OF PHYSALIS PERUVIANA L. FRUIT EXTRACTS ON LEAD ACETATE INTOXICATED RATS

2021 ◽  
Vol 9 (3) ◽  
pp. 353-361
Author(s):  
Revathi C ◽  
◽  
Vadivelu J ◽  
Keyword(s):  
Lead Acetate ◽  
Biochemical Parameters ◽  
Liver Toxicity ◽  
Protective Efficacy ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Physalis Peruviana ◽  
Group I ◽  
Group Ii

Physalis peruviana has been widely used as a medicinal herb for treating various diseases since ancient times. This study aimed to examine the hepatoprotective potential of the ethanolic extract of P. peruviana fruit (EPPF) against lead acetate (LA) intoxicated male albino rats. The experimental rats were divided into ten groups of 4 animals. Animal of Group I served as normal, Group II animals were administered orally 15 mg/Kg body weight of LA, Group III to V animals received EPPF 150mg/kg, 300 mg/kg and 600 mg/kg respectively, Group VI animals received standard silymarin 50 mg/kg, and Group VII to X were treated with LA (15 mg/kg) with EPPF 150, 300, 600 and std 50 mg/kg, orally for 32 days respectively. The degree of protection was measured by estimating hematological parameters such as Hb, RBC, WBC, PCV, platelets, MCV, MCHC, and ESR and biochemical parameters such as urea, creatinine, Cholesterol, HDL, LDL, VLDL, bilirubin, SGOT, SGBT, ALP, GGT, protein, albumin and globulin. Lead acetate induced alterations of hematological and biochemical parameters were observed in group II animals and these levels brought back to normal in the animals treated with EPPF at the concentrations of 150, 300 and 600 mg/kg which was significantly similar to silymarin treated animals. The data of the results obtained depicted that the fruit extract of P. peruviana was found to have promising protective efficacy against lead acetate induced liver toxicity.

Meloxicam induced toxicopathology studies in Wistar rats

2019 ◽  
Author(s):  
S. Pramod Bharani ◽  
A. K. Naik ◽  
S. C. Parija ◽  
S. K. Panda
Keyword(s):  
Wistar Rats ◽  
Hematological Parameters ◽  
Clinical Signs ◽  
High Dose ◽  
Dependent Manner ◽  
Group Iii ◽  
Group I ◽  
Anti Inflammatory ◽  
Group Ii ◽  
Dose Dependent

Nonsteroidal anti-inflammatory drugs (NSAIDs) are the most commonly used class of drugs for treating inflammation and pain. Meloxicam has analgesic, anti-inflammatory and antipyretic properties and is a commonly used NSAID in veterinary practice. The present study was done to evaluate effect of meloxicam on toxico-pathological and hematological parameters in Wistar rats. Eighteen Wistar rats were equally divided into three groups i.e. Group I, Group II and Group III. Group I (negative control) rats received only Normal saline (0.9%) @ 1ml/kg. Group II (Low dose) received meloxicam@ 4 mg/kg B.W. and Group III (High dose) rats received meloxicam@8 mg/kg B.W. orally by gavage for 28 days. Dose-dependent clinical signs and lesions were observed after meloxicam treatment. Kidneys and liver were severely hemorrhagic at the high dose, while intestine and stomach had ulcers and erosions. Hematological values were altered after 28 days of administration. Total Erythrocyte Count (TEC), Packed Cell Volume (PCV), Haemoglobin values were decreased and TLC count was significantly increased in both doses of meloxicam treated groups in a dose-dependent manner. It was concluded that meloxicam caused GIT lesions, nephrotoxicity, hepatotoxicity and variation in the hematological parameters at selected dose and duration.

scholarly journals LOCALIZATION OF ANTIBODIES IN GROUP I AND GROUP II γ-GLOBULINS

1963 ◽  
Vol 118 (5) ◽  
pp. 817-826 ◽  
Author(s):  
Mart Mannik ◽  
Henry G. Kunkel
Keyword(s):  
Teichoic Acid ◽  
Thyroglobulin Antibodies ◽  
Qualitative And Quantitative ◽  
Group I ◽  
Γ Globulins ◽  
Group Ii ◽  
The Individual ◽  
Wide Assortment

A wide assortment of antibodies has been analysed by qualitative and quantitative techniques for their content of group I- and group II-type γ-globulin molecules. These included Rh antibodies, isoagglutinins, thyroglobulin antibodies, dextran antibodies, and teichoic acid antibodies. All the antibodies studied showed the presence of both groups. However, the ratio of the two varied widely in different individuals and diverged markedly from the ratio in the total γ-globulin of the individual. Two antibodies isolated from the serum of the same individual showed different ratios for the two groups.

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