scholarly journals Variability of the structure of winter microbial communities in Chelyabinsk lakes

2021 ◽  
Vol 29 (4) ◽  
pp. 311-318
Author(s):  
S. V. Andreeva ◽  
Y. Y. Filippova ◽  
E. V. Devyatova ◽  
D. Y. Nokhrin
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
Water Bodies ◽  
Coliform Bacteria ◽  
Form Complex ◽  
Freshwater Bodies ◽  
Dynamic Communities ◽  
Chelyabinsk Region ◽  
High Level ◽  
First Time

Microorganisms form complex and dynamic communities that play a key role in the biogeochemical cycles of lakes. A high level of urbanization is currently a serious threat to bacterial communities and the ecosystem of freshwater bodies. To assess the contribution of anthropogenic load to variations in the structure of winter microbial communities in lakes, microorganisms of four water bodies of Chelyabinsk region were studied for the first time. We used cultural, chromatography-mass spectrometric, and modern methods of statistical data processing (particularly, multivariate exploratory analysis and canonical analysis of correspondences). The research showed that the composition of winter microbial communities in lakes Chebarkul’, Smolino, Pervoye, and Shershenevskoye Reservoir did not differ significantly between the main phyla of microorganisms. The dominant microorganisms were found to be of the Firmicutes phylum and Actinobacteria phylum. The structure of bacterial communities had special features depending on the characteristics of the water body and the sampling depths. Thus, in the lakes Smolino, Pervoye, and Shershenevskoye Reservoir, an important role was played by associations between microorganisms – indicators of fecal contamination: coliform bacteria and Enterococcus. On the contrary, in Chebarkul’ Lake, members of the genus Bacillus, which are natural bioremediators, formed stable winter associations. However, the differences between water bodies and sampling depths reflected 28.1% and 9.8% of the variability of the winter microbial communities, respectively. The largest contribution (about 60%) to the variability of the structure was made by intra-water processes, which determined the high heterogeneity of samples from different water areas. We assume that an important role in this variability was played by the high anthropogenic impact in a large industrial metropolis. In our opinion, this line of research is very promising for addressing key environmental issues.

scholarly journals Yearly microbial cycle of human exposed surfaces in show caves

2019 ◽  
Vol 31 ◽  
pp. 1-14
Author(s):  
Silviu Bercea ◽  
Ruxandra Năstase-Bucur ◽  
Oana Teodora Moldovan ◽  
Marius Kenesz ◽  
Silviu Constantin
Keyword(s):  
Microbial Communities ◽  
Rapid Test ◽  
Coliform Bacteria ◽  
Culturable Bacteria ◽  
Indirect Contact ◽  
Cave Environment ◽  
Show Caves ◽  
High Concentrations ◽  
Bacteria And Fungi ◽  
High Level

The human impact upon the subterranean microbiomes is not only a peril to the cave environment but might also affect future visitors. We focused on the changes that humans induced on the surfaces they came in direct or indirect contact with inside two intensely visited Romanian show caves, by means of commercially available microbial rapid test kits and molecular identification. Overall culturable bacteria abundance in the caves maintained high levels year-round while Enterobacteriaceae, coliform bacteria and Escherichiacoli levels peaked during the touristic season, reaching levels that could pose a threat to the health of the visitors. Culturable fungi abundance usually peaked in the spring, remained at a high level in the summer and started to slowly decrease towards the winter months. Differences were observed between the direct and indirect exposed surfaces, as the later had lower overall levels of bacteria and fungi, with increased Enterobacteriaceae loads. Most of the taxa identified are known biodeteriorants of subterranean surfaces and were previously associated with human altered caves. A Dothideomycete sp. previously unknown to the cave environments was detected. This was the first study to analyse the dynamics of the microbial communities of delicate subterranean surfaces in show caves through the use of commercially available test kits. We revealed that exposed surfaces in show caves, in direct or indirect contact with tourists, are host to high concentrations of cultivable microbes. The touristic activity was shown to influence the abundance and dynamics of the microbial communities inhabiting surfaces of show caves.

scholarly journals The role of land use types and water chemical properties in structuring the microbiomes of a connected lakes system

2018 ◽  
Author(s):  
Sophi Marmen ◽  
Lior Blank ◽  
Ashraf Al-Ashhab ◽  
Assaf Malik ◽  
Lars Ganzert ◽  
...  
Keyword(s):  
Water Quality ◽  
Land Use ◽  
Microbial Communities ◽  
Drainage Basin ◽  
Chemical Properties ◽  
Early Summer ◽  
Water Bodies ◽  
Drainage Basins ◽  
Ecological Changes ◽  
Freshwater Bodies

Lakes and other freshwater bodies are intimately connected to the surrounding land, yet to what extent land-use affects the quality of freshwater and the microbial communities living in various freshwater environments is largely unknown. We address this question through an analysis of the land use surrounding 46 inter-connected lakes located within 7 different drainage basins in northern Germany, and the microbiomes of these lakes during early summer. Lake microbiome structure was not determined by the specific drainage basin or by basin size, and bacterial distribution did not seem to be limited by distance. Instead, land use within the drainage basin could predict, to some extent, NO2+NO3 concentrations in the water, which (together with temperature, chlorophyll a and total phosphorus) affected water microbiome structure. Land use directly surrounding the water bodies, however, had little observable effects on water quality or the microbiome. Several microbial lineages, including environmentally important Cyanobacteria and Verrucomicrobia, were differentially partitioned between the lakes. As the amount of available data on land use (e.g. from remote sensing) increases, identifying relationships between land use, aquatic microbial communities and their effect on water quality will be important to better manage freshwater resources worldwide, e.g. by systemically identifying water bodies prone to ecological changes or the presence of harmful organisms.

scholarly journals Rhizosphere Microbiome of Arid Land Medicinal Plants and Extra Cellular Enzymes Contribute to Their Abundance

2020 ◽  
Vol 8 (2) ◽  
pp. 213 ◽  
Author(s):  
Abdul Latif Khan ◽  
Sajjad Asaf ◽  
Raeid M. M. Abed ◽  
Yen Ning Chai ◽  
Ahmed N. Al-Rawahi ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
Extracellular Enzymes ◽  
Growth Conditions ◽  
Soil Conditions ◽  
Arid Environments ◽  
Operational Taxonomic Units ◽  
Rhizosphere Microbiome ◽  
First Time ◽  
Generation Sequencing

Revealing the unexplored rhizosphere microbiome of plants in arid environments can help in understanding their interactions between microbial communities and plants during harsh growth conditions. Here, we report the first investigation of rhizospheric fungal and bacterial communities of Adenium obesum, Aloe dhufarensis and Cleome austroarabica using next-generation sequencing approaches. A. obesum and A. dhufarensis grows in dry tropical and C. austroarabica in arid conditions of Arabian Peninsula. The results indicated the presence of 121 fungal and 3662 bacterial operational taxonomic units (OTUs) whilst microbial diversity was significantly high in the rhizosphere of A. obesum and A. dhufarensis and low in C. austroarabica. Among fungal phyla, Ascomycota and Basidiomycota were abundantly associated within rhizospheres of all three plants. However, Mucoromycota was only present in the rhizospheres of A. obesum and A. dhufarensis, suggesting a variation in fungal niche on the basis of host and soil types. In case of bacterial communities, Actinobacteria, Proteobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, and Verrucomicrobia were predominant microbial phyla. These results demonstrated varying abundances of microbial structure across different hosts and locations in arid environments. Rhizosphere’s extracellular enzymes analysis revealed varying quantities, where, glucosidase, cellulase, esterase, and 1-aminocyclopropane-1-carboxylate deaminase were significantly higher in the rhizosphere of A. dhufarensis, while phosphatase and indole-acetic acid were highest in the rhizosphere of A. obesum. In conclusion, current findings usher for the first time the core microbial communities in the rhizospheric regions of three arid plants that vary greatly with location, host and soil conditions, and suggest the presence of extracellular enzymes could help in maintaining plant growth during the harsh environmental conditions.

A Confrontation between Two Doctrines: The Birth of Struggle for Hegemony in Hebrew Children's Literature during the 1930s and 1940s

2008 ◽  
Vol 1 (2) ◽  
pp. 139-155 ◽  
Author(s):  
YAEL DARR
Keyword(s):  
Children's Literature ◽  
Educational System ◽  
Single Channel ◽  
Children’S Literature ◽  
The Political ◽  
Literary Creation ◽  
History Of ◽  
High Level ◽  
First Time ◽  
Literary Quality

This article describes a crucial and fundamental stage in the transformation of Hebrew children's literature, during the late 1930s and 1940s, from a single channel of expression to a multi-layered polyphony of models and voices. It claims that for the first time in the history of Hebrew children's literature there took place a doctrinal confrontation between two groups of taste-makers. The article outlines the pedagogical and ideological designs of traditionalist Zionist educators, and suggests how these were challenged by a group of prominent writers of adult poetry, members of the Modernist movement. These writers, it is argued, advocated autonomous literary creation, and insisted on a high level of literary quality. Their intervention not only dramatically changed the repertoire of Hebrew children's literature, but also the rules of literary discourse. The article suggests that, through the Modernists’ polemical efforts, Hebrew children's literature was able to free itself from its position as an apparatus controlled by the political-educational system and to become a dynamic and multi-layered field.

To the flora of сonjugates (Streptophyta, Conjugatophyceae) of the Valdai District area of the National Park «Valdaiskiy» (Novgorod Region, Russia)

2014 ◽  
Vol 48 ◽  
pp. 81-88
Author(s):  
A. F. Luknitskaya
Keyword(s):  
Species Distribution ◽  
National Park ◽  
Species Abundance ◽  
Water Bodies ◽  
Number Of Species ◽  
First Time

76 species, 3 varieties and 1 form from 21 genera of Streptophyta, Conjugatophyceae (Actinotaenium, Bambusina, Closterium, Cosmarium, Cylindrocystis, Euastrum, Gonatozygon, Haplotaenium, Micrasterias, Mougeotia, Netrium, Penium, Planotaenium, Pleurotaenium, Raphidiastrum, Spirogyra, Spirotaenia, Staurastrum, Staurodesmus, Tetmemorus, Xanthidium) were found in the basins of the Valdai District area of the National Park «Valdaiskiy» (Novgorod Region, Russia). The list of species is annotated with data on the species distribution in 55 collecting sites of 29 water bodies of the national park, and species abundance in collected samples according to Luknitskaya (2009). Among above mentioned genera, the genus Cosmarium is represented by the greatest number of species (20). Staurastrum chaetoceros has been found for the first time for the Novgorod Region.

scholarly journals Aberrant Expression of TLR2, TLR7, TLR9, Splicing Variants of TLR4 and MYD88 in Chronic Lymphocytic Leukemia Patients

2021 ◽  
Vol 10 (4) ◽  
pp. 867
Author(s):  
Katarzyna Skorka ◽  
Paulina Wlasiuk ◽  
Agnieszka Karczmarczyk ◽  
Krzysztof Giannopoulos
Keyword(s):  
Bone Marrow ◽  
Chronic Lymphocytic Leukemia ◽  
Cytotoxic T Cells ◽  
Lymphocytic Leukemia ◽  
Aberrant Expression ◽  
Downstream Signaling ◽  
Splicing Variants ◽  
High Level ◽  
Time To First Treatment ◽  
First Time

Functional toll-like receptors (TLRs) could modulate anti-tumor effects by activating inflammatory cytokines and the cytotoxic T-cells response. However, excessive TLR expression could promote tumor progression, since TLR-induced inflammation might stimulate cancer cells expansion into the microenvironment. Myd88 is involved in activation NF-κB through TLRs downstream signaling, hence in the current study we provided, for the first time, a complex characterization of expression of TLR2, TLR4, TLR7, TLR9, and MYD88 as well as their splicing forms in two distinct compartments of the microenvironment of chronic lymphocytic leukemia (CLL): peripheral blood and bone marrow. We found correlations between MYD88 and TLRs expressions in both compartments, indicating their relevant cooperation in CLL. The MYD88 expression was higher in CLL patients compared to healthy volunteers (HVs) (0.1780 vs. 0.128, p < 0.0001). The TLRs expression was aberrant in CLL compared to HVs. Analysis of survival curves revealed a shorter time to first treatment in the group of patients with low level of TLR4(3) expression compared to high level of TLR4(3) expression in bone marrow (13 months vs. 48 months, p = 0.0207). We suggest that TLRs expression is differentially regulated in CLL but is similarly shared between two distinct compartments of the microenvironment.

scholarly journals Diversity and Dynamics of Seaweed Associated Microbial Communities Inhabiting the Lagoon of Venice

2020 ◽  
Vol 8 (11) ◽  
pp. 1657
Author(s):  
Abdul-Salam Juhmani ◽  
Alessandro Vezzi ◽  
Mohammad Wahsha ◽  
Alessandro Buosi ◽  
Fabio De Pascale ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
Host Response ◽  
Environmental Parameters ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Nutrient Concentrations ◽  
Lagoon Of Venice ◽  
Anthropogenic Stressors ◽  
Rich Diversity

Seaweeds are a group of essential photosynthetic organisms that harbor a rich diversity of associated microbial communities with substantial functions related to host health and defense. Environmental and anthropogenic stressors may disrupt the microbial communities and their metabolic activity, leading to host physiological alterations that negatively affect seaweeds’ performance and survival. Here, the bacterial communities associated with one of the most common seaweed, Ulva laetevirens Areshough, were sampled over a year at three sites of the lagoon of Venice affected by different environmental and anthropogenic stressors. Bacterial communities were characterized through Illumina sequencing of the V4 hypervariable region of 16S rRNA genes. The study demonstrated that the seaweed associated bacterial communities at sites impacted by environmental stressors were host-specific and differed significantly from the less affected site. Furthermore, these communities were significantly distinct from those of the surrounding seawater. The bacterial communities’ composition was significantly correlated with environmental parameters (nutrient concentrations, dissolved oxygen saturation, and pH) across sites. This study showed that several more abundant bacteria on U. laetevirens at stressed sites belonged to taxa related to the host response to the stressors. Overall, environmental parameters and anthropogenic stressors were shown to substantially affect seaweed associated bacterial communities, which reflect the host response to environmental variations.

scholarly journals Revealing the constituents of Egypt’s oldest beer using infrared and mass spectrometry

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mohamed A. Farag ◽  
Moamen M. Elmassry ◽  
Masahiro Baba ◽  
Renée Friedman
Keyword(s):  
Large Scale ◽  
Beer Brewing ◽  
Chemical Signatures ◽  
Chemical Determination ◽  
Materials Used ◽  
High Level ◽  
Beer Production ◽  
First Time ◽  
Detailed Chemical

Abstract Previous studies have shown that the Ancient Egyptians used malted wheat and barley as the main ingredients in beer brewing, but the chemical determination of the exact recipe is still lacking. To investigate the constituents of ancient beer, we conducted a detailed IR and GC-MS based metabolite analyses targeting volatile and non-volatile metabolites on the residues recovered from the interior of vats in what is currently the world’s oldest (c. 3600 BCE) installation for large-scale beer production located at the major pre-pharaonic political center at Hierakonpolis, Egypt. In addition to distinguishing the chemical signatures of various flavoring agents, such as dates, a significant result of our analysis is the finding, for the first time, of phosphoric acid in high level probably used as a preservative much like in modern beverages. This suggests that the early brewers had acquired the knowledge needed to efficiently produce and preserve large quantities of beer. This study provides the most detailed chemical profile of an ancient beer using modern spectrometric techniques and providing evidence for the likely starting materials used in beer brewing.

PREVENTION ISSUES OF OCCUPATIONAL AND PRODUCTION-RELATED DISEASES IN MINING INDUSTRY WORKERS

2021 ◽  
Author(s):  
G.S. Agzamova ◽  
◽  
N.U. Ibragimova ◽  
Yu.A. Abdieva ◽  
Keyword(s):  
Health Care ◽  
Metallurgical Plant ◽  
Occupational Diseases ◽  
Mining Industry ◽  
Production Environment ◽  
Cardiovascular Pathology ◽  
Dynamic Observation ◽  
High Level ◽  
Medical Examinations ◽  
First Time

Abstract: Protecting and promoting the health of workers in the mining industry is one of the most important problems of occupational pathology and health care. The structure and levels of prevention of occupational diseases are directly dependent on harmful and adverse factors of the production environment and labor process, adequately reflecting the state of production. Purpose: to study the issues of prevention of occupational and production-related diseases of mining and metallurgical plant workers. Research materials and methods: a dynamic observation of the health status of workers in the main industries of the mining and metallurgical plant (800 workers) was carried out. 92 patients with silicosis were examined. Results: Up to 92.8% of first-time occupational diseases are detected during periodic medical examinations. The prevailing sociomatic pathology is cardiovascular pathology, namely, arterial hypertension and diseases of the musculoskeletal system, mainly osteochondrosis of the spine. Prevalence of silicosis was observed in individuals with little professional experience (from 5 years old), young age and primary detection of patients in stage II silicosis, which was accompanied by respiratory failure. Conclusions: The prevention programme developed will ensure a high level of health care in terms of early diagnosis, rehabilitation and secondary prevention of both occupational and occupational diseases.

Innovation in Slovenian tourism organisations

2015 ◽  
Vol 21 (1) ◽  
pp. 51-62
Author(s):  
Maja Uran Maravić ◽  
Dejan Križaj ◽  
Miha Lesjak
Keyword(s):  
Design Methodology ◽  
Mean Value ◽  
Sample Survey ◽  
Research Activity ◽  
Main Hypothesis ◽  
Innovation Activities ◽  
Innovation Climate ◽  
High Level ◽  
Innovation Practices ◽  
First Time

The purpose – Slovenian tourism organisations must constantly focus on developing variety innovations for organisations. In this paper, we present a study conducted on innovation practices in Slovenian tourism organisations. Design/methodology – In a survey conducted on Slovenia tourism organisations, we obtained data and identified their innovation performance and the innovation climate in their area of business. There are three main hypothesis tested. Findings – The research sample of 41 organisations found that most innovation in tourism organisations came through the introduction of new services (90%), followed by innovation through new organisational methods (73%), and found a high-level climate for innovation. Worse was its assessment of research activity within organisations and cooperation with external institutions (eg. universities and research institutes) and investment in innovation activities within their research and development. Results obtained from the research showed a mean value for the innovation climate-instrument of 3.83 indicating a high innovation climate for the Slovenian tourism companies included in the sample survey. Mostly, (publicly known as) more innovative active organisations responded to our survey. From such results, we find that tourism organisations included in the survey are aware of the importance of innovation, teaching organisations to communicate well and network with other organisations, are adaptable to change and engaged with their own ideas in support of the organisation's management. Originality of the research – The contribution of the research is that it has applied the generic instrument for measuring innovation climate on tourism and the first time climate is measured in Slovenia.

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