scholarly journals ANTIMICROBIAL ACTIVITY OF OIL-BEARING PLANTS LAMIACEAE LINDL. TOWARDS ESCHERICHIA COLI

2016 ◽  
Vol 6 (01) ◽  
pp. 216-236
Author(s):  
L.A. Kotyuk
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Biologically Active ◽  
E Coli ◽  
Twofold Increase ◽  
Ethanol Extracts ◽  
Main Components ◽  
Active Substances

<p> </p><p>The paper relates to study of biological activity of 40% ethanol extracts of <em>Dracocephalum moldavica,</em> <em>Hyssopus officinalis</em>, <em>Satureja hortensis</em>, <em>Lophanthus anisatus</em> and <em>Monarda diduma</em>, grown in Ukrainian Polissya, against a pathogenic agent <em>Escherichia coli</em> UCM – B (ATCC 25922).</p><p>The research proves that ethanol extracts of <em>H. officinalis</em>, <em>D. moldavica, S. hortensis, L. anisatus</em> exert antimicrobial activity as the extracted substances provided a twofold increase in minimum bactericidal concentration (MBC) values against <em>E. coli. </em>Likewise, a twofold increase was observed in minimum inhibitory concentration (MIC) of <em>L. anisatus</em> ethanol extracts. As to <em>M. diduma </em>ethanol extracts, their inhibitory and bactericidal influence on <em>E. coli</em> was not registered.</p><p>Oil-bearing plants (family <em>Lamiaceae), </em>grown in Zhytomyr Polissya, are characterized by antimicrobial properties, attributed to biologically active substances that are formed and accumulated in the plant material. The main components of hyssop essential oil are isopinocamphone (44.43%), pinocamphone (35.49%), myrtenol (5.26 %), germacrene D (3.15 %), pulegone (2.93 %), bicyclogermacrene (1.35 %). In mint anise essential oil prevailed pulegone (59.19%), izomenton (14.34%), bicyclogermacrene (3,21 %), <em>β</em>-kariofilen (2,99 %), menton (2.21 %), 1,6-germacradien-5-ol (1.5 %), isopulegone (1.4 %), in summer savory – carvacrol (89.07%), g-terpinene (3.53%), <em>α</em>-thujone (1.7 %), camphor (1.48 %). The dominant components of moldavian dragonhead essential oil were geranial (26.19%) and neral (22.36%), 2-(1-hydroxy-1-isopropyl)-cyklopentanon (8.29 % ), 2,3-dehydro-1,8-cineole (6.87 %), 3-(1-hydroxy-1-isopropyl) cyklopentanon (6,51 %), nerol (4.74 %), 3-methyl-2-cyclohexane 1-on (2.13 %).<em></em></p><p>The paper draws attention to further more detailed study of ethanol extracts of hyssop, moldavian dragonhead, summer savory, mint anise with the aim of producing antibacterial herbal preparations.</p>

scholarly journals Amtimicrobial activity of essential oil of Melissa officinalis L, Lamiaceae

2005 ◽  
Vol 59 (9-10) ◽  
pp. 243-247 ◽  
Author(s):  
Nada Anicic ◽  
Suzana Dimitrijevic ◽  
Mihailo Ristic ◽  
Slobodan Petrovic ◽  
Slobodan Petrovic
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Dilution Method ◽  
Melissa Officinalis ◽  
E Coli ◽  
Diffusion Technique ◽  
Main Components ◽  
Broth Dilution

The antimicrobial activity of the essential oil of Melissa officinalis was investigated in this paper. The essential oil was obtained by the principle of water and steam and analyzed by GC and GC-MS using FID and MSD. The main components of the oil of Melissa officinalis were geranial (17.30%), neral (14.70%) and citronellal (10.70%). The antimicrobial properties were tested against the following bacterial species: B subtilis, B.cereus Bifidobacterium sp., Corynobacterium sp., E. coli, Klebsiella sp., L. acidophilus, L. fermentum, L. plantarum, L. rhamnosus, Listeria monocytogenes, P. vulgaris, P. aerugenosa, S. enteritidis, Shigella sp., S. aureus, and fungi Candida albicans, Alternarija sp. and Aspergillus niger. The diffusion technique was used for testing: the antimicrobial activity, and the MIC was determined by the broth dilution method. The essential oil of M. officinalis showed high antimicrobial activity.

Growth Inhibition of Escherichia coli O157:H7 and Listeria monocytogenes by Carvacrol and Eugenol Encapsulated in Surfactant Micelles

2005 ◽  
Vol 68 (12) ◽  
pp. 2559-2566 ◽  
Author(s):  
SYLVIA GAYSINSKY ◽  
P. MICHAEL DAVIDSON ◽  
BARRY D. BRUCE ◽  
JOCHEN WEISS
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Growth Inhibition ◽  
Surfactant Concentration ◽  
Escherichia Coli O157 ◽  
Surfactant Micelles ◽  
E Coli ◽  
Oil Components

Growth inhibition of four strains of Escherichia coli O157:H7 (H1730, F4546, 932, and E0019) and Listeria monocytogenes (Scott A, 101, 108, and 310) by essential oil components (carvacrol and eugenol) solubilized in nonionic surfactant micelles (Surfynol 465 and 485W) was investigated. Concentrations of encapsulated essential oil components ranged from 0.02 to 1.25% depending on compound, surfactant type, and surfactant concentration (0.5 to 5%). Eugenol encapsulated in Surfynol 485W micelles was most efficient in inhibiting growth of the pathogens; 1% Surfynol 485W and 0.15% eugenol was sufficient to inhibit growth of all strains of E. coli O157:H7 and three of four strains of L. monocytogenes (Scott A, 310, and 108). The fourth strain, L. monocytogenes 101, was inhibited by 2.5% Surfynol and 0.225% eugenol. One percent Surfynol 485W in combination with 0.025% carvacrol was effective in inhibiting three of four strains of E. coli O157:H7. Strain H1730 was the most resistant strain, requiring 0.3% carvacrol and 5% surfactant for complete inhibition. Growth inhibition of L. monocytogenes by combinations of carvacrol and Surfynol 465 ranged between 0.15 and 0.35% and 1 and 3.75%, respectively. Generally, the antimicrobial activity of Surfynol 465 in combination with eugenol was higher than that for the combination with carvacrol. The potent activity was attributed to increased solubility of essential oil components in the aqueous phase due to the presence of surfactants and improved interactions of antimicrobials with microorganisms.

scholarly journals Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

2022 ◽  
Vol 10 (1) ◽  
pp. 109
Author(s):  
Mohammadreza Pajohi Alamoti ◽  
Behnaz Bazargani-Gilani ◽  
Razzagh Mahmoudi ◽  
Anna Reale ◽  
Babak Pakbin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Multidrug Resistant ◽  
Zataria Multiflora ◽  
Cuminum Cyminum ◽  
Plant Essential Oils ◽  
E Coli ◽  
Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

scholarly journals Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Thymus Serpyllum ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

scholarly journals Antimicrobial Activity Exerted by Total Extracts of Germander

2019 ◽  
Vol 70 (9) ◽  
pp. 3242-3244
Author(s):  
Raul Chioibas ◽  
Razvan Susan ◽  
Monica Susan ◽  
Ovidiu Mederle ◽  
Delia Berceanu Vaduva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Fungal Species ◽  
Polyphenolic Compounds ◽  
Biologically Active ◽  
E Coli ◽  
Methanolic Extracts ◽  
Teucrium Polium ◽  
Active Substances

In the present research, ethanolic and methanolic extracts of Teucrium polium (germander) were studied regarding the content of biologically active substances - in particular polyphenols - antioxidant activity and antibacterial activity. The extracts have been found to be rich in polyphenolic compounds, kaempferol and epichatechin have been found to be present in the highest concentrations. The antioxidant activity evaluated by the method of capturing the free radicals with DPPH revealed values close to those produced by the ascorbic acid. Antimicrobial activity was evaluated by the diffusimetric method and S. aureus and E. coli microorganisms were found to be the most sensitive while P. aeruginosa and fungal species were not sensitive.

scholarly journals Антимікробні властивості Tanacetum balsamita L. (Asteraceae) за інтродукції в Поліссі України

2017 ◽  
Vol 7 (1) ◽  
pp. 52-57
Author(s):  
I. V. Ivashchenko
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Minimal Bactericidal Concentration ◽  
Chromatography Method ◽  
Test Cultures

<p>The paper studies antimicrobial activity of ethanol extract of <em>Tanacetum balsamita</em> L. from the family Asteraceae, tribus Anthemideae, the plant being medicinally and pharmaceutically promising. Antimicrobial properties of <em>Tanacetum balsamita</em> were studied on test-cultures, collected from the Ukrainian Collection of Microorganisms (UCM, Institute of microbiology and virology SAS Ukraine), the test-cultures being of <em>Escherichia coli </em>(coliform bacillius) UCM B-906 (ATCC 25922); <em>Staphilococcus aureus</em> (golden staphylococcus) UCM B-904 (ATCC 25923); <em>Pseudomonas aeruginosa</em> (blue pus bacillus) UCM B-900 (ATCC 9027); <em>Candida albicans</em> UCM Y-1918 (ATCC 885-653). The above microorganisms are test strains used to determine antimicrobial properties of medicines. The antimicrobial effect of the investigated substances was studied by the method of successive dilutions which determines minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The extract has demonstrated selective antimicrobial activity against gram-positive bacteria strains of <em>Staphylococcus aureus</em>. In comparison with the solvent, MIC and MBC values showed an 8-times increase. <em>T.</em><em> </em><em>balsamita</em> extract revealed no bacteriostatic, bacteriocidal/fugicidal effect against gram-negative bacteria stains of <em>Escherichia coli, P. aeruginosa</em> and fungus <em>C. albicans</em>. The antimicrobial activity of the extract against <em>S. aureus</em> may be attributed to the presence of essential oil and phenolic compounds. The major compounds in the essential oil are epoxylynaloolacetate (60.13 %) and b-thujone (29.7 %). The following phenolic compounds have been determined with the high-performance liquid chromatography method: flavonoids – luteolin, apigenin, diosmetin, acacetin, apigenin-7-bioside, acacetin-7-bioside, apigenin-7-glycoside, luteolin-7-glycoside, diosmetin-7-bioside, diosmetin-7-glycoside, diosmetin-7-rhamnoside, and hydroxycinnamic acids – n-coumaric, caffeic, chlorogenic, isochlorogenic. The dominant compound is isochlorogenic acid (35 % of the total phenolic compounds detected). The experimental data obtained gives evidence that further research and application of <em>T. balsamita</em> is promising for creation of new anti-staphylococcus drugs.</p>

Antibacterial activity of cuminaldehyde on food-borne pathogens, the bioactive component of essential oil from Cuminum cyminum L. collected in Thailand

2019 ◽  
Vol 16 (4) ◽  
Author(s):  
Nalin Wongkattiya ◽  
Phanchana Sanguansermsri ◽  
Ian Hamilton Fraser ◽  
Donruedee Sanguansermsri
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Biologically Active ◽  
Cuminum Cyminum ◽  
Major Food ◽  
Food Borne ◽  
Bioactive Component ◽  
Main Components

Abstract Background Cuminum cyminum L., commonly known as cumin, has been traditionally used in Thai traditional medicine and traditional food flavoring. The present study investigated the chemical composition, antimicrobial activity against all tested major food-borne pathogenic bacteria, and bioactive components of essential oil extracted from C. cyminum L. collected in Thailand. Methods The main components of the essential oil were investigated by gas chromatography–mass spectrometry (GC-MS) technique. Antibacterial activities against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Salmonella Typhi were investigated by disk diffusion and microdilution method. The presence of the biologically active antibacterial components was also confirmed by the thin-layer chromatography (TLC)-bioautography. Results The main components of the essential oil investigated by GC-MS were cuminaldehyde (27.10%), beta-pinene (25.04%) and gamma-terpinene (15.68%). The essential oil exhibited antibacterial activity against B. cereus, S. aureus, E. coli and S. Typhi. The essential oil showed the strongest antimicrobial activity against B. cereus with a comparable inhibition zone to tetracycline. TLC confirmed the presence of biologically active antibacterial component in the essential oil against all tested food-borne bacteria. It is further demonstrated that cuminaldehyde was the most active compound in TLC-bioautography which inhibited all of tested bacteria. Conclusions Essential oil extracted from C. cyminum L. exhibited antibacterial activity against all tested major food-borne pathogenic bacteria. Cuminaldehyde is a major bioactive component. Our results suggest that the essential oil extracted from C. cyminum L. could be applied as an alternative natural preservative to control food-borne disease and have the potential for further development of new antibacterial agents.

scholarly journals Essential oils of Thymus pulegioides and Thymus glabrescens from Romania: Chemical composition and antimicrobial activity

2010 ◽  
Vol 75 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Mariana Pavel ◽  
Mihailo Ristic ◽  
Tatjana Stevic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Antimicrobial Properties ◽  
Herbal Drug ◽  
Qualitative And Quantitative Analysis ◽  
Gram Positive Bacteria ◽  
Main Components

The aim of this work was to analyse the chemical composition and antimicrobial properties of essential oils isolated from two wild-growing species of thyme (Thymus pulegioides L. and T. glabrescens Willd.) originating from different locations in Romania. The yield of essential oil was determined according to European Pharmacopoeia standards. Qualitative and quantitative analysis of the oils was performed using GC and GC/MS. The antimicrobial activity was tested by the microdilution technique against Gram-negative and Gram-positive bacteria (Escherichia coli, Salmonella typhimurium, S. enteritisdis, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Bacillus subtilis, Micrococcus luteus, M. flavus and Listeria monocytogenes) and human pathogen yeast Candida albicans. The essential oil of Thymus pulegioides was obtained in a yield of 0.7-1 % (v/d.w. herbal drug) and the main components were carvacrol (50.5-62.6 %), ?-terpinene (9.8-9.9 %) and p-cymene (5.8-7.1 %). The essential oil of T. glabrescens was obtained in a yield of 0.7 (v/d.w. herbal drug) and the main components were geraniol (55.5 %), neryl acetate (11.1 %) and ?-bisabolene (6.7 %). The essential oils inhibited microbial growth at concentrations of 10.8-27 ?l/ml.

scholarly journals GC/MS Analysis and Antibacterial Activity of the Essential Oil of Moroccan Tetraclinis articulata (Vahl) Masters

2019 ◽  
Vol 8 (4) ◽  
pp. 302-307 ◽  
Author(s):  
Halima Rabib ◽  
Sophia Zougagh ◽  
Mohamed Hsain ◽  
Wadi Badri ◽  
Tayeb Koussa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Bornyl Acetate ◽  
Average Yield ◽  
Mountain Area ◽  
Plain Area ◽  
Tetraclinis Articulata

The quality and the variability of the chemical composition and antimicrobial properties of essentials oils isolated from Tetraclinis articulata (Vahl) leaves, which were studied from two different regions that are ecologically various. The average yield of essential oil is 0,56 % for the Ras Elma Tazekka region (mountain) and 0,36% for the Debdou region (plain). An analysis made by GC and GC/ MS identified 22 components in Tetraclinis articulata leaves essential oil in the mountain area and 29 components in the plain area. These major components represent the variability of quantity. The percentages following respectively by the two areas are: Bornyl acetate (34,84%; 32,55%), α-Pinene (11,41%; 18,83%), Camphor (11,24 %; 11,31%), Limonene (11,94 %; 8%) and Borneol (8,35%; 7,79%). The antimicrobial activity of T. articulata essential oil was tested against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli which has been inhibited from the 50μg / mL concentration for the Debdou region (Plain) and 25μg / mL for that of the Ras Elma Tazekka region (Mountain).

scholarly journals Antimicrobial Activity of Selected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 546
Author(s):  
Nikola Puvača ◽  
Jovana Milenković ◽  
Tamara Galonja Galonja Coghill ◽  
Vojislava Bursić ◽  
Aleksandra Petrović ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Tea Tree ◽  
E Coli

The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. Coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

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